Carg16059 (gene) Silver-seed gourd (SMH-JMG-627) v2

Overview


Name	Carg16059
Type	gene
Organism	Cucurbita argyrosperma subsp. argyrosperma cv. SMH-JMG-627 (Silver-seed gourd (SMH-JMG-627) v2)
Description	heavy metal-associated isoprenylated plant protein 43-like
Location	Carg_Chr07: 2120027 .. 2120495 (-)
RNA-Seq Expression	Carg16059
Synteny	Carg16059

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideexonCDS

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGGCGCTGATCAAAACCGTCCTCAAACTCGACATTTCTTGTCAAAAATGCAAGACCAAAGTCCTCAAAGCTGTTACTAGTCTCGAAGGTAGCTCTTTCTCTTTAGTATTAATGGATATATTTTCGTGAATAATTGAGTTTTATTCTCAGTGAATTATTTTTCTGTGTAGACGTGGATAAGGTCGAGACGGATGAAGCGAAAGGGACGATCGGCGTGATTGGGAAGGCGGATCCGTACAAGATAGTTAAAATTACGAGGAAGGCGGTTTCCTCCGCCGGCAAGGTTGTCGAGGTGGTGAGCATTGGACCGCCGCCCAAACCCGACGAGAAGAAGCCGGAGGAGAAGAAACCCGATGGTAAGAAGCCCGACCCGGTACCATGCATATGCGCATGTCCTCCCTACCCGCCTTATGGATCGTCGTACATAGTCGTGCCTCATGAAACCCAACCTTCTTGTTCTATCCTTTAA

mRNA sequence

ATGGCGCTGATCAAAACCGTCCTCAAACTCGACATTTCTTGTCAAAAATGCAAGACCAAAGTCCTCAAAGCTGTTACTAGTCTCGAAGACGTGGATAAGGTCGAGACGGATGAAGCGAAAGGGACGATCGGCGTGATTGGGAAGGCGGATCCGTACAAGATAGTTAAAATTACGAGGAAGGCGGTTTCCTCCGCCGGCAAGGTTGTCGAGGTGGTGAGCATTGGACCGCCGCCCAAACCCGACGAGAAGAAGCCGGAGGAGAAGAAACCCGATGGTAAGAAGCCCGACCCGGTACCATGCATATGCGCATGTCCTCCCTACCCGCCTTATGGATCGTCGTACATAGTCGTGCCTCATGAAACCCAACCTTCTTGTTCTATCCTTTAA

Coding sequence (CDS)

ATGGCGCTGATCAAAACCGTCCTCAAACTCGACATTTCTTGTCAAAAATGCAAGACCAAAGTCCTCAAAGCTGTTACTAGTCTCGAAGACGTGGATAAGGTCGAGACGGATGAAGCGAAAGGGACGATCGGCGTGATTGGGAAGGCGGATCCGTACAAGATAGTTAAAATTACGAGGAAGGCGGTTTCCTCCGCCGGCAAGGTTGTCGAGGTGGTGAGCATTGGACCGCCGCCCAAACCCGACGAGAAGAAGCCGGAGGAGAAGAAACCCGATGGTAAGAAGCCCGACCCGGTACCATGCATATGCGCATGTCCTCCCTACCCGCCTTATGGATCGTCGTACATAGTCGTGCCTCATGAAACCCAACCTTCTTGTTCTATCCTTTAA

Protein sequence

MALIKTVLKLDISCQKCKTKVLKAVTSLEDVDKVETDEAKGTIGVIGKADPYKIVKITRKAVSSAGKVVEVVSIGPPPKPDEKKPEEKKPDGKKPDPVPCICACPPYPPYGSSYIVVPHETQPSCSIL

Homology

[Close]

BLAST of Carg16059 vs. NCBI nr
Match: KAG7026779.1 (hypothetical protein SDJN02_10786, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 250.4 bits (638), Expect = 8.5e-63
Identity = 128/128 (100.00%), Postives = 128/128 (100.00%), Query Frame = 0


Query: 1   MALIKTVLKLDISCQKCKTKVLKAVTSLEDVDKVETDEAKGTIGVIGKADPYKIVKITRK 60
           MALIKTVLKLDISCQKCKTKVLKAVTSLEDVDKVETDEAKGTIGVIGKADPYKIVKITRK
Sbjct: 1   MALIKTVLKLDISCQKCKTKVLKAVTSLEDVDKVETDEAKGTIGVIGKADPYKIVKITRK 60

Query: 61  AVSSAGKVVEVVSIGPPPKPDEKKPEEKKPDGKKPDPVPCICACPPYPPYGSSYIVVPHE 120
           AVSSAGKVVEVVSIGPPPKPDEKKPEEKKPDGKKPDPVPCICACPPYPPYGSSYIVVPHE
Sbjct: 61  AVSSAGKVVEVVSIGPPPKPDEKKPEEKKPDGKKPDPVPCICACPPYPPYGSSYIVVPHE 120

Query: 121 TQPSCSIL 129
           TQPSCSIL
Sbjct: 121 TQPSCSIL 128

[Close]

BLAST of Carg16059 vs. NCBI nr
Match: XP_023518146.1 (heavy metal-associated isoprenylated plant protein 43-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 246.1 bits (627), Expect = 1.6e-61
Identity = 127/128 (99.22%), Postives = 127/128 (99.22%), Query Frame = 0


Query: 1   MALIKTVLKLDISCQKCKTKVLKAVTSLEDVDKVETDEAKGTIGVIGKADPYKIVKITRK 60
           MALIKTVLKLDISCQKCKTKVLKAVTSLEDVDKVETDEAKGTIGVIGKADPYKIVKITRK
Sbjct: 1   MALIKTVLKLDISCQKCKTKVLKAVTSLEDVDKVETDEAKGTIGVIGKADPYKIVKITRK 60

Query: 61  AVSSAGKVVEVVSIGPPPKPDEKKPEEKKPDGKKPDPVPCICACPPYPPYGSSYIVVPHE 120
           AVSSAGKVVEVVSIGPPPKPDEKKPEEKKPDGKKPDPVPCICA PPYPPYGSSYIVVPHE
Sbjct: 61  AVSSAGKVVEVVSIGPPPKPDEKKPEEKKPDGKKPDPVPCICAYPPYPPYGSSYIVVPHE 120

Query: 121 TQPSCSIL 129
           TQPSCSIL
Sbjct: 121 TQPSCSIL 128

[Close]

BLAST of Carg16059 vs. NCBI nr
Match: XP_022962785.1 (heavy metal-associated isoprenylated plant protein 43-like [Cucurbita moschata])

HSP 1 Score: 245.0 bits (624), Expect = 3.6e-61
Identity = 125/128 (97.66%), Postives = 126/128 (98.44%), Query Frame = 0


Query: 1   MALIKTVLKLDISCQKCKTKVLKAVTSLEDVDKVETDEAKGTIGVIGKADPYKIVKITRK 60
           MA+IKTVLKLDISCQKCKTKVLKAVTSLEDVDKVETDEAKGTIGVIGKADPYKIVKITRK
Sbjct: 1   MAMIKTVLKLDISCQKCKTKVLKAVTSLEDVDKVETDEAKGTIGVIGKADPYKIVKITRK 60

Query: 61  AVSSAGKVVEVVSIGPPPKPDEKKPEEKKPDGKKPDPVPCICACPPYPPYGSSYIVVPHE 120
           AVSSAGKVVEVVSIGPPPKPDEKKPEEKKPDGKKPD VPCIC CPPYPPYGSSYIVVPHE
Sbjct: 61  AVSSAGKVVEVVSIGPPPKPDEKKPEEKKPDGKKPDSVPCICTCPPYPPYGSSYIVVPHE 120

Query: 121 TQPSCSIL 129
           TQPSCSIL
Sbjct: 121 TQPSCSIL 128

[Close]

BLAST of Carg16059 vs. NCBI nr
Match: XP_023003318.1 (heavy metal-associated isoprenylated plant protein 43-like [Cucurbita maxima])

HSP 1 Score: 229.2 bits (583), Expect = 2.0e-56
Identity = 120/128 (93.75%), Postives = 122/128 (95.31%), Query Frame = 0


Query: 1   MALIKTVLKLDISCQKCKTKVLKAVTSLEDVDKVETDEAKGTIGVIGKADPYKIVKITRK 60
           MALIKTVLKLDISCQKCKTKVLKAVTSLEDVDKVETDEAKGTIGVIGKADPYKIVKITRK
Sbjct: 1   MALIKTVLKLDISCQKCKTKVLKAVTSLEDVDKVETDEAKGTIGVIGKADPYKIVKITRK 60

Query: 61  AVSSAGKVVEVVSIGPPPKPDEKKPEEKKPDGKKPDPVPCICACPPYPPYGSSYIVVPHE 120
           AVSSAGKVVEVVSIGPPPKPDEKKPE+     KKPDPVPCIC CPPYPPYGSSYIVVP+E
Sbjct: 61  AVSSAGKVVEVVSIGPPPKPDEKKPED-----KKPDPVPCICTCPPYPPYGSSYIVVPYE 120

Query: 121 TQPSCSIL 129
           TQPSCSIL
Sbjct: 121 TQPSCSIL 123

[Close]

BLAST of Carg16059 vs. NCBI nr
Match: XP_038877711.1 (heavy metal-associated isoprenylated plant protein 43-like [Benincasa hispida])

HSP 1 Score: 195.3 bits (495), Expect = 3.3e-46
Identity = 99/128 (77.34%), Postives = 109/128 (85.16%), Query Frame = 0


Query: 1   MALIKTVLKLDISCQKCKTKVLKAVTSLEDVDKVETDEAKGTIGVIGKADPYKIVKITRK 60
           MA  KTVLKLDI+CQKCKTKVLKAVTS+E VDKVETDEAKGT+ V G ADP  IVK TRK
Sbjct: 1   MAQTKTVLKLDIACQKCKTKVLKAVTSIEGVDKVETDEAKGTLSVTGTADPVDIVKRTRK 60

Query: 61  AVSSAGKVVEVVSIGPPPKPDEKKPEEKKPDGKKPDPVPCICACPPYPPYGSSYIVVPHE 120
           A+S AGK+V+VVSIGPPPKP+EKKP++KKPD KKP P PC C CPPYPPY  SY+VVP E
Sbjct: 61  AISCAGKIVDVVSIGPPPKPEEKKPDDKKPDDKKPAPPPCPCPCPPYPPYSGSYVVVPCE 120

Query: 121 TQPSCSIL 129
           T PSCSIL
Sbjct: 121 TNPSCSIL 128

[Close]

BLAST of Carg16059 vs. ExPASy Swiss-Prot
Match: Q9SFF7 (Heavy metal-associated isoprenylated plant protein 43 OS=Arabidopsis thaliana OX=3702 GN=HIPP43 PE=2 SV=1)

HSP 1 Score: 63.9 bits (154), Expect = 1.5e-09
Identity = 47/135 (34.81%), Postives = 72/135 (53.33%), Query Frame = 0


Query: 1   MALIKTVLKLDISCQKCKTKVLKAVTSLEDVDKVETDEAKGTIGVIGKADPY----KIVK 60
           M + K  +K+DI+C KC + +++AVT +E V+ +  DE    + V+G  DP     ++ K
Sbjct: 1   MTVKKVEIKVDINCGKCNSAIMEAVTEIEGVNHISLDEGNSILTVVGTMDPVCVATRLKK 60

Query: 61  ITRKAVSSAGKVVEVVSIGPPPKPDE--KKPEEKKP-DGKKPDPVPCICACPPYPPYGSS 120
           I +K V        ++S+GPPPKP E  K PE +KP     P+P   +C  PPY      
Sbjct: 61  IKQKPV--------IISVGPPPKPPEPPKPPEPEKPKPPPAPEPPKHVCK-PPYCNSCDV 120

Query: 121 YIVVPHETQPSCSIL 129
             V  +E+   C+IL
Sbjct: 121 VSVTTYESGSGCTIL 126

[Close]

BLAST of Carg16059 vs. ExPASy Swiss-Prot
Match: Q9LTE3 (Heavy metal-associated isoprenylated plant protein 12 OS=Arabidopsis thaliana OX=3702 GN=HIPP12 PE=3 SV=1)

HSP 1 Score: 59.7 bits (143), Expect = 2.8e-08
Identity = 45/120 (37.50%), Postives = 66/120 (55.00%), Query Frame = 0


Query: 7   VLKLDISCQKCKTKVLKAVTSLEDVDKVETDEAKGTIGVIGKADPYKIVKITRKAVSSAG 66
           VLKLD+ C+K K K +  V  L  V+ VE  + K T  V G+ D Y IVK  +K   +  
Sbjct: 5   VLKLDVHCEKTKQKAMSTVCCLSGVNSVEVKDGKLT--VTGEIDAYMIVKKLKKICHT-- 64

Query: 67  KVVEVVSIGPPPKPDEKKPEE-KKPDGKKPDPVPCICACPPYPPYGSSYIVVPHETQPSC 126
              E +S+GP  +P++KKP++ KKP+ K PD +      PP PPY  ++    +E   +C
Sbjct: 65  ---EFISVGPVKEPEKKKPDDPKKPETKPPDVI--YHYVPPCPPYYHNFNGCYNEDPNAC 115

[Close]

BLAST of Carg16059 vs. ExPASy Swiss-Prot
Match: Q8GWS3 (Heavy metal-associated isoprenylated plant protein 2 OS=Arabidopsis thaliana OX=3702 GN=HIPP02 PE=3 SV=1)

HSP 1 Score: 56.6 bits (135), Expect = 2.4e-07
Identity = 45/128 (35.16%), Postives = 64/128 (50.00%), Query Frame = 0


Query: 1   MALIKTVLKLDISCQKCKTKVLKAVTSLEDVDKVETDEAKGTIGVIGKADPYKIVKITRK 60
           M   K  +K+DI C+KCK  +++AVT LE V+ V  D+ K  + V+G  DP  + +  +K
Sbjct: 1   MTTKKIEIKVDIDCEKCKHAIMEAVTELEGVNIVSLDQEKSILTVVGTMDPVCVAEQLKK 60

Query: 61  AVSSAGKVVEVVSIGPPPKPDEKKPEEKKPDGKKPDPVPCICACPPYPPYGSSYIVVPHE 120
                 K   V+S+GPP KPD       KPD KK    PC      Y P      V  +E
Sbjct: 61  ----INKKPVVISVGPPKKPD------PKPDPKKD---PCFIPYCYYTP-RDMVSVNTYE 114

Query: 121 TQPSCSIL 129
           +   C+I+
Sbjct: 121 SGSGCTIV 114

[Close]

BLAST of Carg16059 vs. ExPASy Swiss-Prot
Match: O03982 (Heavy metal-associated isoprenylated plant protein 39 OS=Arabidopsis thaliana OX=3702 GN=HIPP39 PE=2 SV=1)

HSP 1 Score: 47.4 bits (111), Expect = 1.4e-04
Identity = 32/93 (34.41%), Postives = 50/93 (53.76%), Query Frame = 0


Query: 5  KTVLKLDISCQKCKTKVLKAVTSLEDVDKVETDEAKGTIGVIGKADPYKIVKITRKAVSS 64
          K VLKLD+   + K K LK V++L  +D +  D  +  + VIG  DP  +V   RK    
Sbjct: 3  KIVLKLDLHDDRAKQKALKTVSTLPGIDSIAMDMKEKKLTVIGTVDPVNVVSKLRK---- 62

Query: 65 AGKVVEVVSIGPPPKPDEKKPEEKKPDGKKPDP 98
             + ++V +GP  +P+++K EE K +G    P
Sbjct: 63 YWPMTDIVLVGPAKEPEKEKKEEPKKEGGGEPP 91

[Close]

BLAST of Carg16059 vs. ExPASy Swiss-Prot
Match: Q9FJH5 (Heavy metal-associated isoprenylated plant protein 3 OS=Arabidopsis thaliana OX=3702 GN=HIPP03 PE=1 SV=1)

HSP 1 Score: 47.4 bits (111), Expect = 1.4e-04
Identity = 42/115 (36.52%), Postives = 59/115 (51.30%), Query Frame = 0


Query: 4   IKTVLKLDISCQKCKTKVLKAVTSLEDVDKVETDEAKGTIGVIGKADPYKIVKITRKAVS 63
           I  VLK+D+ C+ C ++++K V S + V+ V+++ A G + V G  DP   VK+  K   
Sbjct: 26  ITVVLKVDMHCEGCASRIVKCVRSFQGVETVKSESATGKLTVTGALDP---VKLREKLEE 85

Query: 64  SAGKVVEVVSIGPPPK------------PDEKKPEE-KKPDG--KKPDPVPCICA 104
              K V++VS  P PK             D+KK EE KKPD   KKP   P   A
Sbjct: 86  KTKKKVDLVS--PQPKKEKEKENKNKNDEDKKKSEEKKKPDNNDKKPKETPVTTA 135

[Close]

BLAST of Carg16059 vs. ExPASy TrEMBL
Match: A0A6J1HG22 (heavy metal-associated isoprenylated plant protein 43-like OS=Cucurbita moschata OX=3662 GN=LOC111463168 PE=4 SV=1)

HSP 1 Score: 245.0 bits (624), Expect = 1.7e-61
Identity = 125/128 (97.66%), Postives = 126/128 (98.44%), Query Frame = 0


Query: 1   MALIKTVLKLDISCQKCKTKVLKAVTSLEDVDKVETDEAKGTIGVIGKADPYKIVKITRK 60
           MA+IKTVLKLDISCQKCKTKVLKAVTSLEDVDKVETDEAKGTIGVIGKADPYKIVKITRK
Sbjct: 1   MAMIKTVLKLDISCQKCKTKVLKAVTSLEDVDKVETDEAKGTIGVIGKADPYKIVKITRK 60

Query: 61  AVSSAGKVVEVVSIGPPPKPDEKKPEEKKPDGKKPDPVPCICACPPYPPYGSSYIVVPHE 120
           AVSSAGKVVEVVSIGPPPKPDEKKPEEKKPDGKKPD VPCIC CPPYPPYGSSYIVVPHE
Sbjct: 61  AVSSAGKVVEVVSIGPPPKPDEKKPEEKKPDGKKPDSVPCICTCPPYPPYGSSYIVVPHE 120

Query: 121 TQPSCSIL 129
           TQPSCSIL
Sbjct: 121 TQPSCSIL 128

[Close]

BLAST of Carg16059 vs. ExPASy TrEMBL
Match: A0A6J1KW59 (heavy metal-associated isoprenylated plant protein 43-like OS=Cucurbita maxima OX=3661 GN=LOC111496961 PE=4 SV=1)

HSP 1 Score: 229.2 bits (583), Expect = 9.9e-57
Identity = 120/128 (93.75%), Postives = 122/128 (95.31%), Query Frame = 0


Query: 1   MALIKTVLKLDISCQKCKTKVLKAVTSLEDVDKVETDEAKGTIGVIGKADPYKIVKITRK 60
           MALIKTVLKLDISCQKCKTKVLKAVTSLEDVDKVETDEAKGTIGVIGKADPYKIVKITRK
Sbjct: 1   MALIKTVLKLDISCQKCKTKVLKAVTSLEDVDKVETDEAKGTIGVIGKADPYKIVKITRK 60

Query: 61  AVSSAGKVVEVVSIGPPPKPDEKKPEEKKPDGKKPDPVPCICACPPYPPYGSSYIVVPHE 120
           AVSSAGKVVEVVSIGPPPKPDEKKPE+     KKPDPVPCIC CPPYPPYGSSYIVVP+E
Sbjct: 61  AVSSAGKVVEVVSIGPPPKPDEKKPED-----KKPDPVPCICTCPPYPPYGSSYIVVPYE 120

Query: 121 TQPSCSIL 129
           TQPSCSIL
Sbjct: 121 TQPSCSIL 123

[Close]

BLAST of Carg16059 vs. ExPASy TrEMBL
Match: A0A0A0KJ75 (HMA domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_6G504410 PE=4 SV=1)

HSP 1 Score: 186.4 bits (472), Expect = 7.3e-44
Identity = 100/136 (73.53%), Postives = 113/136 (83.09%), Query Frame = 0


Query: 1   MALIKTVLKLDISCQKCKTKVLKAVTSLEDVDKVETDEAKGTIGVIGKADPYKIVKITRK 60
           MA+ KTV+KLDI+CQKCKTKVLKAVT++E VDKVETDEAKGT+ VIG ADP++IVK TRK
Sbjct: 1   MAVTKTVMKLDIACQKCKTKVLKAVTAIEGVDKVETDEAKGTLAVIGTADPFEIVKRTRK 60

Query: 61  AVSSAGKVVEVVSIGPPPKPDEKKP-----EEKKPDGKKPDPVPCIC---ACPPYPPYGS 120
           A++ AGKV +VVSIGPPPKPDEKKP     EEKKP  KKPDP PC C    CPPY  YGS
Sbjct: 61  AIACAGKVADVVSIGPPPKPDEKKPEEKKQEEKKPVDKKPDPPPCPCPPYPCPPY--YGS 120

Query: 121 SYIVVPHETQPSCSIL 129
           SY++VPHET PSCSIL
Sbjct: 121 SYVIVPHETYPSCSIL 134

[Close]

BLAST of Carg16059 vs. ExPASy TrEMBL
Match: A0A1S3B1X1 (heavy metal-associated isoprenylated plant protein 3-like OS=Cucumis melo OX=3656 GN=LOC103485075 PE=4 SV=1)

HSP 1 Score: 172.9 bits (437), Expect = 8.4e-40
Identity = 94/137 (68.61%), Postives = 108/137 (78.83%), Query Frame = 0


Query: 1   MALIKTVLKLDISCQKCKTKVLKAVTSLEDVDKVETDEAKGTIGVIGKADPYKIVKITRK 60
           MA  KTVLKLDI+CQKCKTKVLKAVT++E VDKVETDEAKGT+ V G ADP++IVK TRK
Sbjct: 1   MAQTKTVLKLDIACQKCKTKVLKAVTAIEGVDKVETDEAKGTLAVTGTADPFEIVKRTRK 60

Query: 61  AVSSAGKVVEVVSIGPPPKPDEKKPEEKK------PDGKKPDPVPCICA---CPPYPPYG 120
           A++ AGK+ +VVSIGPPPKP EKKP+EKK       D KKP+P PC C    CPPY  YG
Sbjct: 61  AIACAGKIADVVSIGPPPKPGEKKPDEKKEGDKKPADPKKPEPPPCPCPPYHCPPY--YG 120

Query: 121 SSYIVVPHETQPSCSIL 129
           SSY++VP ET P CSIL
Sbjct: 121 SSYVIVPRETYPPCSIL 135

[Close]

BLAST of Carg16059 vs. ExPASy TrEMBL
Match: A0A6J1GFG8 (heavy metal-associated isoprenylated plant protein 43-like OS=Cucurbita moschata OX=3662 GN=LOC111453697 PE=4 SV=1)

HSP 1 Score: 154.1 bits (388), Expect = 4.0e-34
Identity = 82/129 (63.57%), Postives = 96/129 (74.42%), Query Frame = 0


Query: 1   MALIKTVLKLDISCQKCKTKVLKAVTSLEDVDKVETDEAKGTIGVIGKADPYKIVKITRK 60
           MA+ KT+LKLDI+C KCKTKVLKAVT+LE VDK+E DEAKGT+ V G ADPY IV  TRK
Sbjct: 1   MAMTKTILKLDITCHKCKTKVLKAVTALEGVDKIEMDEAKGTLAVTGTADPYDIVTRTRK 60

Query: 61  AVSSAGKVVEVVSIGPPPKPDEKKPEEKKPDGKKPDPVPCICACPPYPP-YGSSYIVVPH 120
           A+S AGK+ ++ +IGPPPKP+       +P    P   P  C CP YPP YGSSY+VVPH
Sbjct: 61  AISCAGKIADIDTIGPPPKPNPPPKPTPQPSCSCPPFPPPYCPCPSYPPYYGSSYVVVPH 120

Query: 121 ETQPSCSIL 129
           ET PSCSIL
Sbjct: 121 ETYPSCSIL 129

[Close]

BLAST of Carg16059 vs. TAIR 10
Match: AT3G05920.1 (Heavy metal transport/detoxification superfamily protein )

HSP 1 Score: 63.9 bits (154), Expect = 1.1e-10
Identity = 47/135 (34.81%), Postives = 72/135 (53.33%), Query Frame = 0


Query: 1   MALIKTVLKLDISCQKCKTKVLKAVTSLEDVDKVETDEAKGTIGVIGKADPY----KIVK 60
           M + K  +K+DI+C KC + +++AVT +E V+ +  DE    + V+G  DP     ++ K
Sbjct: 1   MTVKKVEIKVDINCGKCNSAIMEAVTEIEGVNHISLDEGNSILTVVGTMDPVCVATRLKK 60

Query: 61  ITRKAVSSAGKVVEVVSIGPPPKPDE--KKPEEKKP-DGKKPDPVPCICACPPYPPYGSS 120
           I +K V        ++S+GPPPKP E  K PE +KP     P+P   +C  PPY      
Sbjct: 61  IKQKPV--------IISVGPPPKPPEPPKPPEPEKPKPPPAPEPPKHVCK-PPYCNSCDV 120

Query: 121 YIVVPHETQPSCSIL 129
             V  +E+   C+IL
Sbjct: 121 VSVTTYESGSGCTIL 126

[Close]

BLAST of Carg16059 vs. TAIR 10
Match: AT5G52740.1 (Copper transport protein family )

HSP 1 Score: 59.7 bits (143), Expect = 2.0e-09
Identity = 45/120 (37.50%), Postives = 66/120 (55.00%), Query Frame = 0


Query: 7   VLKLDISCQKCKTKVLKAVTSLEDVDKVETDEAKGTIGVIGKADPYKIVKITRKAVSSAG 66
           VLKLD+ C+K K K +  V  L  V+ VE  + K T  V G+ D Y IVK  +K   +  
Sbjct: 5   VLKLDVHCEKTKQKAMSTVCCLSGVNSVEVKDGKLT--VTGEIDAYMIVKKLKKICHT-- 64

Query: 67  KVVEVVSIGPPPKPDEKKPEE-KKPDGKKPDPVPCICACPPYPPYGSSYIVVPHETQPSC 126
              E +S+GP  +P++KKP++ KKP+ K PD +      PP PPY  ++    +E   +C
Sbjct: 65  ---EFISVGPVKEPEKKKPDDPKKPETKPPDVI--YHYVPPCPPYYHNFNGCYNEDPNAC 115

[Close]

BLAST of Carg16059 vs. TAIR 10
Match: AT5G26690.1 (Heavy metal transport/detoxification superfamily protein )

HSP 1 Score: 56.6 bits (135), Expect = 1.7e-08
Identity = 45/128 (35.16%), Postives = 64/128 (50.00%), Query Frame = 0


Query: 1   MALIKTVLKLDISCQKCKTKVLKAVTSLEDVDKVETDEAKGTIGVIGKADPYKIVKITRK 60
           M   K  +K+DI C+KCK  +++AVT LE V+ V  D+ K  + V+G  DP  + +  +K
Sbjct: 1   MTTKKIEIKVDIDCEKCKHAIMEAVTELEGVNIVSLDQEKSILTVVGTMDPVCVAEQLKK 60

Query: 61  AVSSAGKVVEVVSIGPPPKPDEKKPEEKKPDGKKPDPVPCICACPPYPPYGSSYIVVPHE 120
                 K   V+S+GPP KPD       KPD KK    PC      Y P      V  +E
Sbjct: 61  ----INKKPVVISVGPPKKPD------PKPDPKKD---PCFIPYCYYTP-RDMVSVNTYE 114

Query: 121 TQPSCSIL 129
           +   C+I+
Sbjct: 121 SGSGCTIV 114

[Close]

BLAST of Carg16059 vs. TAIR 10
Match: AT1G01490.1 (Heavy metal transport/detoxification superfamily protein )

HSP 1 Score: 47.4 bits (111), Expect = 1.0e-05
Identity = 32/93 (34.41%), Postives = 50/93 (53.76%), Query Frame = 0


Query: 5  KTVLKLDISCQKCKTKVLKAVTSLEDVDKVETDEAKGTIGVIGKADPYKIVKITRKAVSS 64
          K VLKLD+   + K K LK V++L  +D +  D  +  + VIG  DP  +V   RK    
Sbjct: 3  KIVLKLDLHDDRAKQKALKTVSTLPGIDSIAMDMKEKKLTVIGTVDPVNVVSKLRK---- 62

Query: 65 AGKVVEVVSIGPPPKPDEKKPEEKKPDGKKPDP 98
             + ++V +GP  +P+++K EE K +G    P
Sbjct: 63 YWPMTDIVLVGPAKEPEKEKKEEPKKEGGGEPP 91

[Close]

BLAST of Carg16059 vs. TAIR 10
Match: AT1G01490.2 (Heavy metal transport/detoxification superfamily protein )

HSP 1 Score: 47.4 bits (111), Expect = 1.0e-05
Identity = 32/93 (34.41%), Postives = 50/93 (53.76%), Query Frame = 0


Query: 5  KTVLKLDISCQKCKTKVLKAVTSLEDVDKVETDEAKGTIGVIGKADPYKIVKITRKAVSS 64
          K VLKLD+   + K K LK V++L  +D +  D  +  + VIG  DP  +V   RK    
Sbjct: 3  KIVLKLDLHDDRAKQKALKTVSTLPGIDSIAMDMKEKKLTVIGTVDPVNVVSKLRK---- 62

Query: 65 AGKVVEVVSIGPPPKPDEKKPEEKKPDGKKPDP 98
             + ++V +GP  +P+++K EE K +G    P
Sbjct: 63 YWPMTDIVLVGPAKEPEKEKKEEPKKEGGGEPP 91

The following BLAST results are available for this feature:

BLAST of Carg16059 vs. NCBI nr


Match Name	E-value	Identity	Description
KAG7026779.1	8.5e-63	100.00	hypothetical protein SDJN02_10786, partial [Cucurbita argyrosperma subsp. argyro...	[more]
XP_023518146.1	1.6e-61	99.22	heavy metal-associated isoprenylated plant protein 43-like [Cucurbita pepo subsp...	[more]
XP_022962785.1	3.6e-61	97.66	heavy metal-associated isoprenylated plant protein 43-like [Cucurbita moschata]	[more]
XP_023003318.1	2.0e-56	93.75	heavy metal-associated isoprenylated plant protein 43-like [Cucurbita maxima]	[more]
XP_038877711.1	3.3e-46	77.34	heavy metal-associated isoprenylated plant protein 43-like [Benincasa hispida]	[more]

BLAST of Carg16059 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q9SFF7	1.5e-09	34.81	Heavy metal-associated isoprenylated plant protein 43 OS=Arabidopsis thaliana OX...	[more]
Q9LTE3	2.8e-08	37.50	Heavy metal-associated isoprenylated plant protein 12 OS=Arabidopsis thaliana OX...	[more]
Q8GWS3	2.4e-07	35.16	Heavy metal-associated isoprenylated plant protein 2 OS=Arabidopsis thaliana OX=...	[more]
O03982	1.4e-04	34.41	Heavy metal-associated isoprenylated plant protein 39 OS=Arabidopsis thaliana OX...	[more]
Q9FJH5	1.4e-04	36.52	Heavy metal-associated isoprenylated plant protein 3 OS=Arabidopsis thaliana OX=...	[more]

BLAST of Carg16059 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1HG22	1.7e-61	97.66	heavy metal-associated isoprenylated plant protein 43-like OS=Cucurbita moschata...	[more]
A0A6J1KW59	9.9e-57	93.75	heavy metal-associated isoprenylated plant protein 43-like OS=Cucurbita maxima O...	[more]
A0A0A0KJ75	7.3e-44	73.53	HMA domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_6G504410 PE=4 SV...	[more]
A0A1S3B1X1	8.4e-40	68.61	heavy metal-associated isoprenylated plant protein 3-like OS=Cucumis melo OX=365...	[more]
A0A6J1GFG8	4.0e-34	63.57	heavy metal-associated isoprenylated plant protein 43-like OS=Cucurbita moschata...	[more]

BLAST of Carg16059 vs. TAIR 10


Match Name	E-value	Identity	Description
AT3G05920.1	1.1e-10	34.81	Heavy metal transport/detoxification superfamily protein	[more]
AT5G52740.1	2.0e-09	37.50	Copper transport protein family	[more]
AT5G26690.1	1.7e-08	35.16	Heavy metal transport/detoxification superfamily protein	[more]
AT1G01490.1	1.0e-05	34.41	Heavy metal transport/detoxification superfamily protein	[more]
AT1G01490.2	1.0e-05	34.41	Heavy metal transport/detoxification superfamily protein	[more]

InterPro

Analysis Name: InterPro Annotations of Silver-seed gourd (SMH-JMG-627) v2
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR006121	Heavy metal-associated domain, HMA	PFAM	PF00403	HMA	coord: 11..67 e-value: 3.0E-8 score: 33.9
None	No IPR available	GENE3D	3.30.70.100		coord: 3..77 e-value: 5.9E-13 score: 50.8
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 72..98
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 77..95
None	No IPR available	PANTHER	PTHR45811	COPPER TRANSPORT PROTEIN FAMILY-RELATED	coord: 4..127
None	No IPR available	PANTHER	PTHR45811:SF13	OS04G0661100 PROTEIN	coord: 4..127
IPR036163	Heavy metal-associated domain superfamily	SUPERFAMILY	55008	HMA, heavy metal-associated domain	coord: 5..61

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Carg16059-RA	Carg16059-RA	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
molecular_function	GO:0046872	metal ion binding