Carg16016 (gene) Silver-seed gourd (SMH-JMG-627) v2

Overview


Name	Carg16016
Type	gene
Organism	Cucurbita argyrosperma subsp. argyrosperma cv. SMH-JMG-627 (Silver-seed gourd (SMH-JMG-627) v2)
Description	aspartic proteinase-like protein 1 isoform X1
Location	Carg_Chr07: 2339063 .. 2340075 (+)
RNA-Seq Expression	Carg16016
Synteny	Carg16016

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRCDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

TCTGTCTGCTTCTTTACACTTCACAACTCTTCTTCTTCCTAACTTCCGGCTGAACTCGTCGGTTTCTGTGATCTCTCCTCCATAATTTCGGCGGTCCGTAAACTCGAAAGCTTGCTGTTTCTGTTTCTCATTTTGGTCCCGGGCGGAAATTGAGGGTCTGAATTGTTGGCAGTTTCTTCTGAAGCTCCAGATCTCGACTTCGAGCTGCTTCGTTCGTCCTTTGAGGATTGGTTGCAATGTCGCTTCGGAATCTGGTTTTGGTCCTGCTGATGATGATTTCCGCTCACCAGGCTATGTCGACTGCGTTTACATCGAGAATACTTCACCGGTTCTCTGAGGAGTTGAAGGCGCTTAGGGTTTCAAGGAGTACGAATACGAGTGTACGAGTCTCGTGGCCTGAGAAAGGGAGCATGGAGTATTATCAGGAGCTTGTGAGCGGTGACTTCCAGAGGCAGAAGATGAAGCTTGGCTCTCGGTTTCAGTTGCTTTTCCCGTCTGAAGGCAGCAAAACCATTGCACTGGGGAATGACTTTGGCTGGTCACCACTAATCTACCTTCTCCTTTTTTCTTTTTTGCCTACCCTGTTCTTGCAATACTTTGTTCTTCTGTTTTGAATATCCTTTTCTGTCTGTTTTTCCTCTTACACCATGATTTATCCGCAAACCTTTTTCCTTTGAGTAATCCCCATCCCCCCTAAAATAAAATAAGAAATCCCCACCTCACTAATAGCCTTTTGCATAGTGGCACCTTTATCTGAAAATTTTGTCTTGTGGTATGATTTATGCACCTGATGAATCAATTTGTTATTTACCTCTTATAGGTTGCATTACGCCTGGATCGACATCGGGACGCCAAGTGTTTCATTTCTGGTTGCATTGGATGCCGGGAGTGATCTGCTTTGGGTTCCTTGTGATTGCATACAATGTGCTCCTTTGTCTGCAAGTTACTATGGCAGTCTGGTAGGCCTGCTTTTCGGAATCTGTTCTTTCTACATAGCCATTAATTACTTCTAA

mRNA sequence

TCTGTCTGCTTCTTTACACTTCACAACTCTTCTTCTTCCTAACTTCCGGCTGAACTCGTCGGTTTCTGTGATCTCTCCTCCATAATTTCGGCGGTCCGTAAACTCGAAAGCTTGCTGTTTCTGTTTCTCATTTTGGTCCCGGGCGGAAATTGAGGGTCTGAATTGTTGGCAGTTTCTTCTGAAGCTCCAGATCTCGACTTCGAGCTGCTTCGTTCGTCCTTTGAGGATTGGTTGCAATGTCGCTTCGGAATCTGGTTTTGGTCCTGCTGATGATGATTTCCGCTCACCAGGCTATGTCGACTGCGTTTACATCGAGAATACTTCACCGGTTCTCTGAGGAGTTGAAGGCGCTTAGGGTTTCAAGGAGTACGAATACGAGTGTACGAGTCTCGTGGCCTGAGAAAGGGAGCATGGAGTATTATCAGGAGCTTGTGAGCGGTGACTTCCAGAGGCAGAAGATGAAGCTTGGCTCTCGGTTTCAGTTGCTTTTCCCGTCTGAAGGCAGCAAAACCATTGCACTGGGGAATGACTTTGGCTGGTTGCATTACGCCTGGATCGACATCGGGACGCCAAGTGTTTCATTTCTGGTTGCATTGGATGCCGGGAGTGATCTGCTTTGGGTTCCTTGTGATTGCATACAATGTGCTCCTTTGTCTGCAAGTTACTATGGCAGTCTGGTAGGCCTGCTTTTCGGAATCTGTTCTTTCTACATAGCCATTAATTACTTCTAA

Coding sequence (CDS)

ATGTCGCTTCGGAATCTGGTTTTGGTCCTGCTGATGATGATTTCCGCTCACCAGGCTATGTCGACTGCGTTTACATCGAGAATACTTCACCGGTTCTCTGAGGAGTTGAAGGCGCTTAGGGTTTCAAGGAGTACGAATACGAGTGTACGAGTCTCGTGGCCTGAGAAAGGGAGCATGGAGTATTATCAGGAGCTTGTGAGCGGTGACTTCCAGAGGCAGAAGATGAAGCTTGGCTCTCGGTTTCAGTTGCTTTTCCCGTCTGAAGGCAGCAAAACCATTGCACTGGGGAATGACTTTGGCTGGTTGCATTACGCCTGGATCGACATCGGGACGCCAAGTGTTTCATTTCTGGTTGCATTGGATGCCGGGAGTGATCTGCTTTGGGTTCCTTGTGATTGCATACAATGTGCTCCTTTGTCTGCAAGTTACTATGGCAGTCTGGTAGGCCTGCTTTTCGGAATCTGTTCTTTCTACATAGCCATTAATTACTTCTAA

Protein sequence

MSLRNLVLVLLMMISAHQAMSTAFTSRILHRFSEELKALRVSRSTNTSVRVSWPEKGSMEYYQELVSGDFQRQKMKLGSRFQLLFPSEGSKTIALGNDFGWLHYAWIDIGTPSVSFLVALDAGSDLLWVPCDCIQCAPLSASYYGSLVGLLFGICSFYIAINYF

Homology

[Close]

BLAST of Carg16016 vs. NCBI nr
Match: KAG7026822.1 (Aspartic proteinase-like protein 1 [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 324.7 bits (831), Expect = 4.6e-85
Identity = 164/164 (100.00%), Postives = 164/164 (100.00%), Query Frame = 0


Query: 1   MSLRNLVLVLLMMISAHQAMSTAFTSRILHRFSEELKALRVSRSTNTSVRVSWPEKGSME 60
           MSLRNLVLVLLMMISAHQAMSTAFTSRILHRFSEELKALRVSRSTNTSVRVSWPEKGSME
Sbjct: 1   MSLRNLVLVLLMMISAHQAMSTAFTSRILHRFSEELKALRVSRSTNTSVRVSWPEKGSME 60

Query: 61  YYQELVSGDFQRQKMKLGSRFQLLFPSEGSKTIALGNDFGWLHYAWIDIGTPSVSFLVAL 120
           YYQELVSGDFQRQKMKLGSRFQLLFPSEGSKTIALGNDFGWLHYAWIDIGTPSVSFLVAL
Sbjct: 61  YYQELVSGDFQRQKMKLGSRFQLLFPSEGSKTIALGNDFGWLHYAWIDIGTPSVSFLVAL 120

Query: 121 DAGSDLLWVPCDCIQCAPLSASYYGSLVGLLFGICSFYIAINYF 165
           DAGSDLLWVPCDCIQCAPLSASYYGSLVGLLFGICSFYIAINYF
Sbjct: 121 DAGSDLLWVPCDCIQCAPLSASYYGSLVGLLFGICSFYIAINYF 164

[Close]

BLAST of Carg16016 vs. NCBI nr
Match: KAG6594861.1 (Aspartic proteinase-like protein 1, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 289.7 bits (740), Expect = 1.6e-74
Identity = 145/147 (98.64%), Postives = 146/147 (99.32%), Query Frame = 0


Query: 1   MSLRNLVLVLLMMISAHQAMSTAFTSRILHRFSEELKALRVSRSTNTSVRVSWPEKGSME 60
           MSLRNLVLVLLMMISAHQAMS AFTSRILHRFSEE+KALRVSRSTNTSVRVSWPEKGSME
Sbjct: 1   MSLRNLVLVLLMMISAHQAMSIAFTSRILHRFSEEMKALRVSRSTNTSVRVSWPEKGSME 60

Query: 61  YYQELVSGDFQRQKMKLGSRFQLLFPSEGSKTIALGNDFGWLHYAWIDIGTPSVSFLVAL 120
           YYQELVSGDFQRQKMKLGSRFQLLFPSEGSKTIALGNDFGWLHYAWIDIGTPSVSFLVAL
Sbjct: 61  YYQELVSGDFQRQKMKLGSRFQLLFPSEGSKTIALGNDFGWLHYAWIDIGTPSVSFLVAL 120

Query: 121 DAGSDLLWVPCDCIQCAPLSASYYGSL 148
           DAGSDLLWVPCDCIQCAPLSASYYGSL
Sbjct: 121 DAGSDLLWVPCDCIQCAPLSASYYGSL 147

[Close]

BLAST of Carg16016 vs. NCBI nr
Match: XP_022963039.1 (aspartic proteinase-like protein 1 isoform X3 [Cucurbita moschata])

HSP 1 Score: 289.3 bits (739), Expect = 2.1e-74
Identity = 144/147 (97.96%), Postives = 146/147 (99.32%), Query Frame = 0


Query: 1   MSLRNLVLVLLMMISAHQAMSTAFTSRILHRFSEELKALRVSRSTNTSVRVSWPEKGSME 60
           MSLRNLVLVLLMMISAHQAMS AFTSRILHRFSEE+KALRVSRSTNTSVRVSWPEKGSME
Sbjct: 1   MSLRNLVLVLLMMISAHQAMSIAFTSRILHRFSEEMKALRVSRSTNTSVRVSWPEKGSME 60

Query: 61  YYQELVSGDFQRQKMKLGSRFQLLFPSEGSKTIALGNDFGWLHYAWIDIGTPSVSFLVAL 120
           YYQELVSGDFQRQKMKLGSRFQLLFPSEGSKTIALGNDFGWLHYAWIDIGTPSVSFLVAL
Sbjct: 61  YYQELVSGDFQRQKMKLGSRFQLLFPSEGSKTIALGNDFGWLHYAWIDIGTPSVSFLVAL 120

Query: 121 DAGSDLLWVPCDCIQCAPLSASYYGSL 148
           DAGSDLLW+PCDCIQCAPLSASYYGSL
Sbjct: 121 DAGSDLLWIPCDCIQCAPLSASYYGSL 147

[Close]

BLAST of Carg16016 vs. NCBI nr
Match: XP_022963037.1 (aspartic proteinase-like protein 1 isoform X1 [Cucurbita moschata])

HSP 1 Score: 289.3 bits (739), Expect = 2.1e-74
Identity = 144/147 (97.96%), Postives = 146/147 (99.32%), Query Frame = 0


Query: 1   MSLRNLVLVLLMMISAHQAMSTAFTSRILHRFSEELKALRVSRSTNTSVRVSWPEKGSME 60
           MSLRNLVLVLLMMISAHQAMS AFTSRILHRFSEE+KALRVSRSTNTSVRVSWPEKGSME
Sbjct: 1   MSLRNLVLVLLMMISAHQAMSIAFTSRILHRFSEEMKALRVSRSTNTSVRVSWPEKGSME 60

Query: 61  YYQELVSGDFQRQKMKLGSRFQLLFPSEGSKTIALGNDFGWLHYAWIDIGTPSVSFLVAL 120
           YYQELVSGDFQRQKMKLGSRFQLLFPSEGSKTIALGNDFGWLHYAWIDIGTPSVSFLVAL
Sbjct: 61  YYQELVSGDFQRQKMKLGSRFQLLFPSEGSKTIALGNDFGWLHYAWIDIGTPSVSFLVAL 120

Query: 121 DAGSDLLWVPCDCIQCAPLSASYYGSL 148
           DAGSDLLW+PCDCIQCAPLSASYYGSL
Sbjct: 121 DAGSDLLWIPCDCIQCAPLSASYYGSL 147

[Close]

BLAST of Carg16016 vs. NCBI nr
Match: XP_023003199.1 (aspartic proteinase-like protein 1 isoform X1 [Cucurbita maxima])

HSP 1 Score: 288.5 bits (737), Expect = 3.6e-74
Identity = 144/147 (97.96%), Postives = 146/147 (99.32%), Query Frame = 0


Query: 1   MSLRNLVLVLLMMISAHQAMSTAFTSRILHRFSEELKALRVSRSTNTSVRVSWPEKGSME 60
           MSLRNLVLVLLMMISAHQA+S AFTSRILHRFSEE+KALRVSRSTNTSVRVSWPEKGSME
Sbjct: 1   MSLRNLVLVLLMMISAHQALSIAFTSRILHRFSEEMKALRVSRSTNTSVRVSWPEKGSME 60

Query: 61  YYQELVSGDFQRQKMKLGSRFQLLFPSEGSKTIALGNDFGWLHYAWIDIGTPSVSFLVAL 120
           YYQELVSGDFQRQKMKLGSRFQLLFPSEGSKTIALGNDFGWLHYAWIDIGTPSVSFLVAL
Sbjct: 61  YYQELVSGDFQRQKMKLGSRFQLLFPSEGSKTIALGNDFGWLHYAWIDIGTPSVSFLVAL 120

Query: 121 DAGSDLLWVPCDCIQCAPLSASYYGSL 148
           DAGSDLLWVPCDCIQCAPLSASYYGSL
Sbjct: 121 DAGSDLLWVPCDCIQCAPLSASYYGSL 147

[Close]

BLAST of Carg16016 vs. ExPASy Swiss-Prot
Match: Q9LX20 (Aspartic proteinase-like protein 1 OS=Arabidopsis thaliana OX=3702 GN=At5g10080 PE=2 SV=1)

HSP 1 Score: 169.1 bits (427), Expect = 4.2e-41
Identity = 78/141 (55.32%), Postives = 110/141 (78.01%), Query Frame = 0


Query: 7   VLVLLMMISAHQAMSTAFTSRILHRFSEELKALRVSRSTNTSVRVSWPEKGSMEYYQELV 66
           +L  ++ ++  + +++ F+SR++HRFS+E +A   + S++ S+    P K S+EYY+ L 
Sbjct: 8   LLFCVLFLATEETLASLFSSRLIHRFSDEGRASIKTPSSSDSL----PNKQSLEYYRLLA 67

Query: 67  SGDFQRQKMKLGSRFQLLFPSEGSKTIALGNDFGWLHYAWIDIGTPSVSFLVALDAGSDL 126
             DF+RQ+M LG++ Q L PSEGSKTI+ GNDFGWLHY WIDIGTPSVSFLVALD GS+L
Sbjct: 68  ESDFRRQRMNLGAKVQSLVPSEGSKTISSGNDFGWLHYTWIDIGTPSVSFLVALDTGSNL 127

Query: 127 LWVPCDCIQCAPLSASYYGSL 148
           LW+PC+C+QCAPL+++YY SL
Sbjct: 128 LWIPCNCVQCAPLTSTYYSSL 144

[Close]

BLAST of Carg16016 vs. ExPASy Swiss-Prot
Match: Q8VYV9 (Aspartyl protease family protein 1 OS=Arabidopsis thaliana OX=3702 GN=APF1 PE=2 SV=1)

HSP 1 Score: 68.2 bits (165), Expect = 1.0e-10
Identity = 46/141 (32.62%), Postives = 74/141 (52.48%), Query Frame = 0


Query: 2   SLRNLVLVLLMMISAHQAMSTA-----FTSRILHRFSEELKALRVSRSTNTSVRVSWPEK 61
           S R L L LL+++++   +        F     HRFS+++  +              P +
Sbjct: 6   SCRILFLGLLILLASSWVLDRCEGFGEFGFEFHHRFSDQVVGVLPGDGL--------PNR 65

Query: 62  GSMEYYQELVSGDFQRQKMKLGSRFQLLFP-SEGSKTIALGNDFGWLHYAWIDIGTPSVS 121
            S +YY+ +   D   +  +L +  Q L   S+G++T+ + +  G+LHYA + +GTPS  
Sbjct: 66  DSSKYYRVMAHRDRLIRGRRLANEDQSLVTFSDGNETVRV-DALGFLHYANVTVGTPSDW 125

Query: 122 FLVALDAGSDLLWVPCDCIQC 137
           F+VALD GSDL W+PCDC  C
Sbjct: 126 FMVALDTGSDLFWLPCDCTNC 137

[Close]

BLAST of Carg16016 vs. ExPASy Swiss-Prot
Match: Q766C2 (Aspartic proteinase nepenthesin-2 OS=Nepenthes gracilis OX=150966 GN=nep2 PE=1 SV=1)

HSP 1 Score: 45.1 bits (105), Expect = 9.2e-04
Identity = 26/74 (35.14%), Postives = 38/74 (51.35%), Query Frame = 0


Query: 64  ELVSGDFQRQKMKLGSRFQLLFPSEGSKTIALGNDFGWLHYAWIDIGTPSVSFLVALDAG 123
           EL+    +R + ++ S   +L  S G +T     D  +L    + IGTP  SF   +D G
Sbjct: 59  ELIKRAIKRGERRMRSINAMLQSSSGIETPVYAGDGEYLMN--VAIGTPDSSFSAIMDTG 118

Query: 124 SDLLWVPCD-CIQC 137
           SDL+W  C+ C QC
Sbjct: 119 SDLIWTQCEPCTQC 130

[Close]

BLAST of Carg16016 vs. ExPASy TrEMBL
Match: A0A6J1HIV4 (aspartic proteinase-like protein 1 isoform X3 OS=Cucurbita moschata OX=3662 GN=LOC111463362 PE=3 SV=1)

HSP 1 Score: 289.3 bits (739), Expect = 1.0e-74
Identity = 144/147 (97.96%), Postives = 146/147 (99.32%), Query Frame = 0


Query: 1   MSLRNLVLVLLMMISAHQAMSTAFTSRILHRFSEELKALRVSRSTNTSVRVSWPEKGSME 60
           MSLRNLVLVLLMMISAHQAMS AFTSRILHRFSEE+KALRVSRSTNTSVRVSWPEKGSME
Sbjct: 1   MSLRNLVLVLLMMISAHQAMSIAFTSRILHRFSEEMKALRVSRSTNTSVRVSWPEKGSME 60

Query: 61  YYQELVSGDFQRQKMKLGSRFQLLFPSEGSKTIALGNDFGWLHYAWIDIGTPSVSFLVAL 120
           YYQELVSGDFQRQKMKLGSRFQLLFPSEGSKTIALGNDFGWLHYAWIDIGTPSVSFLVAL
Sbjct: 61  YYQELVSGDFQRQKMKLGSRFQLLFPSEGSKTIALGNDFGWLHYAWIDIGTPSVSFLVAL 120

Query: 121 DAGSDLLWVPCDCIQCAPLSASYYGSL 148
           DAGSDLLW+PCDCIQCAPLSASYYGSL
Sbjct: 121 DAGSDLLWIPCDCIQCAPLSASYYGSL 147

[Close]

BLAST of Carg16016 vs. ExPASy TrEMBL
Match: A0A6J1HE55 (aspartic proteinase-like protein 1 isoform X1 OS=Cucurbita moschata OX=3662 GN=LOC111463362 PE=3 SV=1)

HSP 1 Score: 289.3 bits (739), Expect = 1.0e-74
Identity = 144/147 (97.96%), Postives = 146/147 (99.32%), Query Frame = 0


Query: 1   MSLRNLVLVLLMMISAHQAMSTAFTSRILHRFSEELKALRVSRSTNTSVRVSWPEKGSME 60
           MSLRNLVLVLLMMISAHQAMS AFTSRILHRFSEE+KALRVSRSTNTSVRVSWPEKGSME
Sbjct: 1   MSLRNLVLVLLMMISAHQAMSIAFTSRILHRFSEEMKALRVSRSTNTSVRVSWPEKGSME 60

Query: 61  YYQELVSGDFQRQKMKLGSRFQLLFPSEGSKTIALGNDFGWLHYAWIDIGTPSVSFLVAL 120
           YYQELVSGDFQRQKMKLGSRFQLLFPSEGSKTIALGNDFGWLHYAWIDIGTPSVSFLVAL
Sbjct: 61  YYQELVSGDFQRQKMKLGSRFQLLFPSEGSKTIALGNDFGWLHYAWIDIGTPSVSFLVAL 120

Query: 121 DAGSDLLWVPCDCIQCAPLSASYYGSL 148
           DAGSDLLW+PCDCIQCAPLSASYYGSL
Sbjct: 121 DAGSDLLWIPCDCIQCAPLSASYYGSL 147

[Close]

BLAST of Carg16016 vs. ExPASy TrEMBL
Match: A0A6J1KSL9 (aspartic proteinase-like protein 1 isoform X1 OS=Cucurbita maxima OX=3661 GN=LOC111496886 PE=3 SV=1)

HSP 1 Score: 288.5 bits (737), Expect = 1.8e-74
Identity = 144/147 (97.96%), Postives = 146/147 (99.32%), Query Frame = 0


Query: 1   MSLRNLVLVLLMMISAHQAMSTAFTSRILHRFSEELKALRVSRSTNTSVRVSWPEKGSME 60
           MSLRNLVLVLLMMISAHQA+S AFTSRILHRFSEE+KALRVSRSTNTSVRVSWPEKGSME
Sbjct: 1   MSLRNLVLVLLMMISAHQALSIAFTSRILHRFSEEMKALRVSRSTNTSVRVSWPEKGSME 60

Query: 61  YYQELVSGDFQRQKMKLGSRFQLLFPSEGSKTIALGNDFGWLHYAWIDIGTPSVSFLVAL 120
           YYQELVSGDFQRQKMKLGSRFQLLFPSEGSKTIALGNDFGWLHYAWIDIGTPSVSFLVAL
Sbjct: 61  YYQELVSGDFQRQKMKLGSRFQLLFPSEGSKTIALGNDFGWLHYAWIDIGTPSVSFLVAL 120

Query: 121 DAGSDLLWVPCDCIQCAPLSASYYGSL 148
           DAGSDLLWVPCDCIQCAPLSASYYGSL
Sbjct: 121 DAGSDLLWVPCDCIQCAPLSASYYGSL 147

[Close]

BLAST of Carg16016 vs. ExPASy TrEMBL
Match: A0A6J1KNG7 (aspartic proteinase-like protein 1 isoform X3 OS=Cucurbita maxima OX=3661 GN=LOC111496886 PE=3 SV=1)

HSP 1 Score: 288.5 bits (737), Expect = 1.8e-74
Identity = 144/147 (97.96%), Postives = 146/147 (99.32%), Query Frame = 0


Query: 1   MSLRNLVLVLLMMISAHQAMSTAFTSRILHRFSEELKALRVSRSTNTSVRVSWPEKGSME 60
           MSLRNLVLVLLMMISAHQA+S AFTSRILHRFSEE+KALRVSRSTNTSVRVSWPEKGSME
Sbjct: 1   MSLRNLVLVLLMMISAHQALSIAFTSRILHRFSEEMKALRVSRSTNTSVRVSWPEKGSME 60

Query: 61  YYQELVSGDFQRQKMKLGSRFQLLFPSEGSKTIALGNDFGWLHYAWIDIGTPSVSFLVAL 120
           YYQELVSGDFQRQKMKLGSRFQLLFPSEGSKTIALGNDFGWLHYAWIDIGTPSVSFLVAL
Sbjct: 61  YYQELVSGDFQRQKMKLGSRFQLLFPSEGSKTIALGNDFGWLHYAWIDIGTPSVSFLVAL 120

Query: 121 DAGSDLLWVPCDCIQCAPLSASYYGSL 148
           DAGSDLLWVPCDCIQCAPLSASYYGSL
Sbjct: 121 DAGSDLLWVPCDCIQCAPLSASYYGSL 147

[Close]

BLAST of Carg16016 vs. ExPASy TrEMBL
Match: A0A5D3CLH5 (Aspartic proteinase-like protein 1 isoform X1 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold255G002590 PE=3 SV=1)

HSP 1 Score: 270.8 bits (691), Expect = 3.8e-69
Identity = 133/147 (90.48%), Postives = 141/147 (95.92%), Query Frame = 0


Query: 1   MSLRNLVLVLLMMISAHQAMSTAFTSRILHRFSEELKALRVSRSTNTSVRVSWPEKGSME 60
           MSLRNLV++LLM+I  HQA+S  FTSRILHRFSEE+KALRVS STNTSVRVSWPEKGSME
Sbjct: 1   MSLRNLVMLLLMVIFVHQAVSITFTSRILHRFSEEMKALRVSVSTNTSVRVSWPEKGSME 60

Query: 61  YYQELVSGDFQRQKMKLGSRFQLLFPSEGSKTIALGNDFGWLHYAWIDIGTPSVSFLVAL 120
           YYQELVSGDF+RQKMKLGSRFQLLFPSEGSKTIALGNDFGWLHY WIDIGTPSVSFLVAL
Sbjct: 61  YYQELVSGDFRRQKMKLGSRFQLLFPSEGSKTIALGNDFGWLHYTWIDIGTPSVSFLVAL 120

Query: 121 DAGSDLLWVPCDCIQCAPLSASYYGSL 148
           DAGSDLLW+PC+CIQCAPLSASYYGSL
Sbjct: 121 DAGSDLLWIPCNCIQCAPLSASYYGSL 147

[Close]

BLAST of Carg16016 vs. TAIR 10
Match: AT5G10080.1 (Eukaryotic aspartyl protease family protein )

HSP 1 Score: 169.1 bits (427), Expect = 3.0e-42
Identity = 78/141 (55.32%), Postives = 110/141 (78.01%), Query Frame = 0


Query: 7   VLVLLMMISAHQAMSTAFTSRILHRFSEELKALRVSRSTNTSVRVSWPEKGSMEYYQELV 66
           +L  ++ ++  + +++ F+SR++HRFS+E +A   + S++ S+    P K S+EYY+ L 
Sbjct: 8   LLFCVLFLATEETLASLFSSRLIHRFSDEGRASIKTPSSSDSL----PNKQSLEYYRLLA 67

Query: 67  SGDFQRQKMKLGSRFQLLFPSEGSKTIALGNDFGWLHYAWIDIGTPSVSFLVALDAGSDL 126
             DF+RQ+M LG++ Q L PSEGSKTI+ GNDFGWLHY WIDIGTPSVSFLVALD GS+L
Sbjct: 68  ESDFRRQRMNLGAKVQSLVPSEGSKTISSGNDFGWLHYTWIDIGTPSVSFLVALDTGSNL 127

Query: 127 LWVPCDCIQCAPLSASYYGSL 148
           LW+PC+C+QCAPL+++YY SL
Sbjct: 128 LWIPCNCVQCAPLTSTYYSSL 144

[Close]

BLAST of Carg16016 vs. TAIR 10
Match: AT4G35880.1 (Eukaryotic aspartyl protease family protein )

HSP 1 Score: 93.2 bits (230), Expect = 2.1e-19
Identity = 56/145 (38.62%), Postives = 81/145 (55.86%), Query Frame = 0


Query: 7   VLVLLMMISAHQAMSTAFTSRILHRFSEELKALRVSRSTNTSVRVSWPEKGSMEYYQELV 66
           ++ +LM++S        FT  + HRFS+E+K      S +T     +P KGS EY+  LV
Sbjct: 12  LIPILMLLSFGSCNGRIFTFEMHHRFSDEVK----QWSDSTGRFAKFPPKGSFEYFNALV 71

Query: 67  SGDFQRQKMKLG-----SRFQLLFPSEGSKTIALGNDFGWLHYAWIDIGTPSVSFLVALD 126
             D+  +  +L      S   L F S+G+ T  + +  G+LHY  + +GTP + F+VALD
Sbjct: 72  LRDWLIRGRRLSESESESESSLTF-SDGNSTSRI-SSLGFLHYTTVKLGTPGMRFMVALD 131

Query: 127 AGSDLLWVPCDCIQCAPLSASYYGS 147
            GSDL WVPCDC +CAP   + Y S
Sbjct: 132 TGSDLFWVPCDCGKCAPTEGATYAS 150

[Close]

BLAST of Carg16016 vs. TAIR 10
Match: AT3G51360.1 (Eukaryotic aspartyl protease family protein )

HSP 1 Score: 75.9 bits (185), Expect = 3.4e-14
Identity = 44/122 (36.07%), Postives = 66/122 (54.10%), Query Frame = 0


Query: 12  MMISAHQAMSTAFTSRILHRFSEELKALRVSRSTNTSVRVSWPEKGSMEYYQELVSGDFQ 71
           M +    ++S + +  I HRFSE++K +              PE GS++YY+ LV  D  
Sbjct: 10  MSLGLASSVSGSLSFEIHHRFSEQVKTVLGGHGL--------PEMGSLDYYKALVHRDRG 69

Query: 72  RQKMKLGSRFQLLFPSEGSKTIALGNDFGWLHYAWIDIGTPSVSFLVALDAGSDLLWVPC 131
           RQ     +    +  ++G+ T     +  +LHYA + IGTP+  FLVALD GSDL W+PC
Sbjct: 70  RQLTSNNNNQTTISFAQGNST----EEISFLHYANVTIGTPAQWFLVALDTGSDLFWLPC 119

Query: 132 DC 134
           +C
Sbjct: 130 NC 119

[Close]

BLAST of Carg16016 vs. TAIR 10
Match: AT3G51330.1 (Eukaryotic aspartyl protease family protein )

HSP 1 Score: 71.2 bits (173), Expect = 8.5e-13
Identity = 46/116 (39.66%), Postives = 67/116 (57.76%), Query Frame = 0


Query: 21  STAFTSRILHRFSEELKALRVSRSTNTSVRVSWPEKGSMEYYQELVSGDFQRQKMKLGSR 80
           S  F+  + H FS+     RV +S      V  PEKGS+EY++ L   D   +   L S 
Sbjct: 26  SGKFSFEVHHMFSD-----RVKQSLGLDDLV--PEKGSLEYFKVLAQRDRLIRGRGLASN 85

Query: 81  FQ---LLFPSEGSKTIALGNDFGWLHYAWIDIGTPSVSFLVALDAGSDLLWVPCDC 134
            +   + F   G++TI++ +  G+LHYA + +GTP+  FLVALD GSDL W+PC+C
Sbjct: 86  NEETPITF-MRGNRTISI-DLLGFLHYANVSVGTPATWFLVALDTGSDLFWLPCNC 132

[Close]

BLAST of Carg16016 vs. TAIR 10
Match: AT3G51340.1 (Eukaryotic aspartyl protease family protein )

HSP 1 Score: 70.5 bits (171), Expect = 1.4e-12
Identity = 36/81 (44.44%), Postives = 48/81 (59.26%), Query Frame = 0


Query: 54  PEKGSMEYYQELVSGD-FQRQKMKLGSRFQLLFPSEGSKTIALGNDFGWLHYAWIDIGTP 113
           PE GS+EY++ L   D F R +    +  +    S GS      N  G+LHYA + +GTP
Sbjct: 53  PENGSLEYFKVLAHRDRFIRGRGLASNNEETPLTSIGSNLTLALNFLGFLHYANVSLGTP 112

Query: 114 SVSFLVALDAGSDLLWVPCDC 134
           +  FLVALD GSDL W+PC+C
Sbjct: 113 ATWFLVALDTGSDLFWLPCNC 133

The following BLAST results are available for this feature:

BLAST of Carg16016 vs. NCBI nr


Match Name	E-value	Identity	Description
KAG7026822.1	4.6e-85	100.00	Aspartic proteinase-like protein 1 [Cucurbita argyrosperma subsp. argyrosperma]	[more]
KAG6594861.1	1.6e-74	98.64	Aspartic proteinase-like protein 1, partial [Cucurbita argyrosperma subsp. soror...	[more]
XP_022963039.1	2.1e-74	97.96	aspartic proteinase-like protein 1 isoform X3 [Cucurbita moschata]	[more]
XP_022963037.1	2.1e-74	97.96	aspartic proteinase-like protein 1 isoform X1 [Cucurbita moschata]	[more]
XP_023003199.1	3.6e-74	97.96	aspartic proteinase-like protein 1 isoform X1 [Cucurbita maxima]	[more]

BLAST of Carg16016 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q9LX20	4.2e-41	55.32	Aspartic proteinase-like protein 1 OS=Arabidopsis thaliana OX=3702 GN=At5g10080 ...	[more]
Q8VYV9	1.0e-10	32.62	Aspartyl protease family protein 1 OS=Arabidopsis thaliana OX=3702 GN=APF1 PE=2 ...	[more]
Q766C2	9.2e-04	35.14	Aspartic proteinase nepenthesin-2 OS=Nepenthes gracilis OX=150966 GN=nep2 PE=1 S...	[more]

BLAST of Carg16016 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1HIV4	1.0e-74	97.96	aspartic proteinase-like protein 1 isoform X3 OS=Cucurbita moschata OX=3662 GN=L...	[more]
A0A6J1HE55	1.0e-74	97.96	aspartic proteinase-like protein 1 isoform X1 OS=Cucurbita moschata OX=3662 GN=L...	[more]
A0A6J1KSL9	1.8e-74	97.96	aspartic proteinase-like protein 1 isoform X1 OS=Cucurbita maxima OX=3661 GN=LOC...	[more]
A0A6J1KNG7	1.8e-74	97.96	aspartic proteinase-like protein 1 isoform X3 OS=Cucurbita maxima OX=3661 GN=LOC...	[more]
A0A5D3CLH5	3.8e-69	90.48	Aspartic proteinase-like protein 1 isoform X1 OS=Cucumis melo var. makuwa OX=119...	[more]

BLAST of Carg16016 vs. TAIR 10


Match Name	E-value	Identity	Description
AT5G10080.1	3.0e-42	55.32	Eukaryotic aspartyl protease family protein	[more]
AT4G35880.1	2.1e-19	38.62	Eukaryotic aspartyl protease family protein	[more]
AT3G51360.1	3.4e-14	36.07	Eukaryotic aspartyl protease family protein	[more]
AT3G51330.1	8.5e-13	39.66	Eukaryotic aspartyl protease family protein	[more]
AT3G51340.1	1.4e-12	44.44	Eukaryotic aspartyl protease family protein	[more]

InterPro

Analysis Name: InterPro Annotations of Silver-seed gourd (SMH-JMG-627) v2
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR033121	Peptidase family A1 domain	PFAM	PF00026	Asp	coord: 104..133 e-value: 3.9E-4 score: 19.9
IPR033121	Peptidase family A1 domain	PROSITE	PS51767	PEPTIDASE_A1	coord: 103..164 score: 13.113728
IPR021109	Aspartic peptidase domain superfamily	GENE3D	2.40.70.10	Acid Proteases	coord: 56..147 e-value: 1.2E-10 score: 43.5
IPR021109	Aspartic peptidase domain superfamily	SUPERFAMILY	50630	Acid proteases	coord: 87..145
IPR001461	Aspartic peptidase A1 family	PANTHER	PTHR13683	ASPARTYL PROTEASES	coord: 4..145
None	No IPR available	PANTHER	PTHR13683:SF339	EUKARYOTIC ASPARTYL PROTEASE FAMILY PROTEIN	coord: 4..145

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Carg16016-RA	Carg16016-RA	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0006508	proteolysis
molecular_function	GO:0004190	aspartic-type endopeptidase activity