Carg16014 (gene) Silver-seed gourd (SMH-JMG-627) v2

Overview


Name	Carg16014
Type	gene
Organism	Cucurbita argyrosperma subsp. argyrosperma cv. SMH-JMG-627 (Silver-seed gourd (SMH-JMG-627) v2)
Description	heavy metal-associated isoprenylated plant protein 23-like
Location	Carg_Chr07: 2345160 .. 2346983 (+)
RNA-Seq Expression	Carg16014
Synteny	Carg16014

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRCDSpolypeptidethree_prime_UTR

Hold the cursor over a type above to highlight its positions in the sequence below.

AATTTTTGAAAAATTGAAAATTTAGATCAGTTCATGGGTTGATTTTTTGTTTTTTTCGAGTAGATCCTACCAACCTGAAAATAGGGTTACAATCTCACTCAACCCTACTAAATTTTTAAGATTACGAAGATTAATAATATGGTAGATAAATAAGATTTATTAAATAAATGAAAAATAACTCAACAACCCAATTCAAGTTTGGTTGAGGTGTGAACTCTATTCGAGTTGCTCGACCACCTACCAACCAACTCGAAATTTTGGATTGTTTTCATATATACCCCTAATTCAAATTCTTCTGTGGCAAACATTTATACATAATCCATCATTGACAGCTCAAAGAGTTTCTACTTAAAAAAGGGCAATCATCTTCCCAAGAATGGAGCCAAAAGTGCCTCTTCATCGAGAAAAATGTATAAAAATCTTCATCATCTTAAAACAACAAAAACCAAGCTTCCTAAGCCAAATTATTTTTGGTGATTGGGGGGCCACAACTACAGCACAACCCCTTTTCCTCTGTTTTCAATCTCACACAAACGCCTATATAATAAGCAACCCCTGTTTCATGAAACCATCTCAAAACCAGAGCAAGAAACACACAAAACAGCCTTTCAAAATGCTCTTTACTTTGTTTGTTTGAAGGGAAGCTAATAAGATCCATCATTTCCACCCAAACTTACCTTCCCATTTTCTCTACATCTCAATTCTCAACTTTCAGATCATCATTTTTCTTCCCCCCTGACCAAACATTGAAGGTTTCTCTGGGTTTTCCTCCTGTGAGCTTCTGAAACTTTCAGTTTTCAACTTCATCCATTAAAGCGTGAAATGGGTGTTGGTGGGACTCTTGAGTACTTATCAGATTTGGTGGGAAGTAGCCATAAGCACAAGAAAAAGAAACAGCTTCAAACTGTGGAGCTGAAGGTAAGAATGGACTGTGATGGCTGTGAGCTCAAGGTCAAGAATGCCCTCTCTTCACTCAGTGGTATTTCTCGAGAAATTCCTTTTTTATCTCACTTTTATTGTTTTCTCTTTGTAAACTTGTCCATTTCAAGAGAGATTACTAACAAAAAATGTGGCGTGTTTGAAGGGGTGAAATCTGTGGAGATAAACAGAAAGCAGCAGAAGGTGACAGTTACAGGGTATGTTGAAGCAAGCAAGGTGCTTAAGAAGGCTAAGTCCACAGGGAAGAAGGCAGAGATATGGCCGTATGTTCCCTACAGTTTAGTGGCGCAGCCTTACATTGCTCAGGCTTATGACAAGAAAGCACCACCAGGCTACGTTAGAAATGTGGAGCAGACTGCCATTACTGCTTCTGTTACCAAATATGAGGACCCTTACATGAACATGTTCAGCGATGACAACCCAAATGCCTGCTCTATCATGTAATTCTAGTACAAGAAGTTCGTGTTTTGTATCACTCTCTGTCTTACTTCAGCTTATTTTTTGTCATCAGCTCAATAATTTGTAGTGGAATCCTTTTATGAAGAGAAAGGGTCAACTTGGGATGACTGATTAGAACATTTGGGGTTGTTAGTTGTTAGTAGTGATGTAAGAGATTACTTTATATCTCTTGGTTTGAAGGATGCATGATGTTGTAATTTCTTGTTTGTGAGATGACTTTGGTTTTCTCCACAGCTCTTCTTTCACCTAAAAAAGGATTCCAACCTGGTTATTAACTGTTTAGAAGACTGAAAATGTAGAGTTGGAGAGGAGAACGAAGCATTCCTTATATGGGTGTGGAAACCTCTCCCTAGCATACACGTTTTAAAATCGTGAGACTGACGACGATACGTAACAGGCCAAAGCGGACAATATCTACTAGCGATA

mRNA sequence

AATTTTTGAAAAATTGAAAATTTAGATCAGTTCATGGGTTGATTTTTTGTTTTTTTCGAGTAGATCCTACCAACCTGAAAATAGGGTTACAATCTCACTCAACCCTACTAAATTTTTAAGATTACGAAGATTAATAATATGGTAGATAAATAAGATTTATTAAATAAATGAAAAATAACTCAACAACCCAATTCAAGTTTGGTTGAGGTGTGAACTCTATTCGAGTTGCTCGACCACCTACCAACCAACTCGAAATTTTGGATTGTTTTCATATATACCCCTAATTCAAATTCTTCTGTGGCAAACATTTATACATAATCCATCATTGACAGCTCAAAGAGTTTCTACTTAAAAAAGGGCAATCATCTTCCCAAGAATGGAGCCAAAAGTGCCTCTTCATCGAGAAAAATGTATAAAAATCTTCATCATCTTAAAACAACAAAAACCAAGCTTCCTAAGCCAAATTATTTTTGGTGATTGGGGGGCCACAACTACAGCACAACCCCTTTTCCTCTGTTTTCAATCTCACACAAACGCCTATATAATAAGCAACCCCTGTTTCATGAAACCATCTCAAAACCAGAGCAAGAAACACACAAAACAGCCTTTCAAAATGCTCTTTACTTTGTTTGTTTGAAGGGAAGCTAATAAGATCCATCATTTCCACCCAAACTTACCTTCCCATTTTCTCTACATCTCAATTCTCAACTTTCAGATCATCATTTTTCTTCCCCCCTGACCAAACATTGAAGGTTTCTCTGGGTTTTCCTCCTGTGAGCTTCTGAAACTTTCAGTTTTCAACTTCATCCATTAAAGCGTGAAATGGGTGTTGGTGGGACTCTTGAGTACTTATCAGATTTGGTGGGAAGTAGCCATAAGCACAAGAAAAAGAAACAGCTTCAAACTGTGGAGCTGAAGGTAAGAATGGACTGTGATGGCTGTGAGCTCAAGGTCAAGAATGCCCTCTCTTCACTCAGTGGGGTGAAATCTGTGGAGATAAACAGAAAGCAGCAGAAGGTGACAGTTACAGGGTATGTTGAAGCAAGCAAGGTGCTTAAGAAGGCTAAGTCCACAGGGAAGAAGGCAGAGATATGGCCGTATGTTCCCTACAGTTTAGTGGCGCAGCCTTACATTGCTCAGGCTTATGACAAGAAAGCACCACCAGGCTACGTTAGAAATGTGGAGCAGACTGCCATTACTGCTTCTGTTACCAAATATGAGGACCCTTACATGAACATGTTCAGCGATGACAACCCAAATGCCTGCTCTATCATGTAATTCTAGTACAAGAAGTTCGTGTTTTGTATCACTCTCTGTCTTACTTCAGCTTATTTTTTGTCATCAGCTCAATAATTTGTAGTGGAATCCTTTTATGAAGAGAAAGGGTCAACTTGGGATGACTGATTAGAACATTTGGGGTTGTTAGTTGTTAGTAGTGATGTAAGAGATTACTTTATATCTCTTGGTTTGAAGGATGCATGATGTTGTAATTTCTTGTTTGTGAGATGACTTTGGTTTTCTCCACAGCTCTTCTTTCACCTAAAAAAGGATTCCAACCTGGTTATTAACTGTTTAGAAGACTGAAAATGTAGAGTTGGAGAGGAGAACGAAGCATTCCTTATATGGGTGTGGAAACCTCTCCCTAGCATACACGTTTTAAAATCGTGAGACTGACGACGATACGTAACAGGCCAAAGCGGACAATATCTACTAGCGATA

Coding sequence (CDS)

ATGGGTGTTGGTGGGACTCTTGAGTACTTATCAGATTTGGTGGGAAGTAGCCATAAGCACAAGAAAAAGAAACAGCTTCAAACTGTGGAGCTGAAGGTAAGAATGGACTGTGATGGCTGTGAGCTCAAGGTCAAGAATGCCCTCTCTTCACTCAGTGGGGTGAAATCTGTGGAGATAAACAGAAAGCAGCAGAAGGTGACAGTTACAGGGTATGTTGAAGCAAGCAAGGTGCTTAAGAAGGCTAAGTCCACAGGGAAGAAGGCAGAGATATGGCCGTATGTTCCCTACAGTTTAGTGGCGCAGCCTTACATTGCTCAGGCTTATGACAAGAAAGCACCACCAGGCTACGTTAGAAATGTGGAGCAGACTGCCATTACTGCTTCTGTTACCAAATATGAGGACCCTTACATGAACATGTTCAGCGATGACAACCCAAATGCCTGCTCTATCATGTAA

Protein sequence

MGVGGTLEYLSDLVGSSHKHKKKKQLQTVELKVRMDCDGCELKVKNALSSLSGVKSVEINRKQQKVTVTGYVEASKVLKKAKSTGKKAEIWPYVPYSLVAQPYIAQAYDKKAPPGYVRNVEQTAITASVTKYEDPYMNMFSDDNPNACSIM

Homology

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BLAST of Carg16014 vs. NCBI nr
Match: XP_022962722.1 (heavy metal-associated isoprenylated plant protein 23-like [Cucurbita moschata] >XP_023003202.1 heavy metal-associated isoprenylated plant protein 23-like [Cucurbita maxima] >XP_023517668.1 heavy metal-associated isoprenylated plant protein 23-like [Cucurbita pepo subsp. pepo] >KAG7026824.1 Heavy metal-associated isoprenylated plant protein 23 [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 301.2 bits (770), Expect = 5.0e-78
Identity = 151/151 (100.00%), Postives = 151/151 (100.00%), Query Frame = 0


Query: 1   MGVGGTLEYLSDLVGSSHKHKKKKQLQTVELKVRMDCDGCELKVKNALSSLSGVKSVEIN 60
           MGVGGTLEYLSDLVGSSHKHKKKKQLQTVELKVRMDCDGCELKVKNALSSLSGVKSVEIN
Sbjct: 1   MGVGGTLEYLSDLVGSSHKHKKKKQLQTVELKVRMDCDGCELKVKNALSSLSGVKSVEIN 60

Query: 61  RKQQKVTVTGYVEASKVLKKAKSTGKKAEIWPYVPYSLVAQPYIAQAYDKKAPPGYVRNV 120
           RKQQKVTVTGYVEASKVLKKAKSTGKKAEIWPYVPYSLVAQPYIAQAYDKKAPPGYVRNV
Sbjct: 61  RKQQKVTVTGYVEASKVLKKAKSTGKKAEIWPYVPYSLVAQPYIAQAYDKKAPPGYVRNV 120

Query: 121 EQTAITASVTKYEDPYMNMFSDDNPNACSIM 152
           EQTAITASVTKYEDPYMNMFSDDNPNACSIM
Sbjct: 121 EQTAITASVTKYEDPYMNMFSDDNPNACSIM 151

[Close]

BLAST of Carg16014 vs. NCBI nr
Match: KAG6594861.1 (Aspartic proteinase-like protein 1, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 301.2 bits (770), Expect = 5.0e-78
Identity = 151/151 (100.00%), Postives = 151/151 (100.00%), Query Frame = 0


Query: 1   MGVGGTLEYLSDLVGSSHKHKKKKQLQTVELKVRMDCDGCELKVKNALSSLSGVKSVEIN 60
           MGVGGTLEYLSDLVGSSHKHKKKKQLQTVELKVRMDCDGCELKVKNALSSLSGVKSVEIN
Sbjct: 527 MGVGGTLEYLSDLVGSSHKHKKKKQLQTVELKVRMDCDGCELKVKNALSSLSGVKSVEIN 586

Query: 61  RKQQKVTVTGYVEASKVLKKAKSTGKKAEIWPYVPYSLVAQPYIAQAYDKKAPPGYVRNV 120
           RKQQKVTVTGYVEASKVLKKAKSTGKKAEIWPYVPYSLVAQPYIAQAYDKKAPPGYVRNV
Sbjct: 587 RKQQKVTVTGYVEASKVLKKAKSTGKKAEIWPYVPYSLVAQPYIAQAYDKKAPPGYVRNV 646

Query: 121 EQTAITASVTKYEDPYMNMFSDDNPNACSIM 152
           EQTAITASVTKYEDPYMNMFSDDNPNACSIM
Sbjct: 647 EQTAITASVTKYEDPYMNMFSDDNPNACSIM 677

[Close]

BLAST of Carg16014 vs. NCBI nr
Match: XP_038882614.1 (heavy metal-associated isoprenylated plant protein 23 [Benincasa hispida])

HSP 1 Score: 294.7 bits (753), Expect = 4.7e-76
Identity = 147/151 (97.35%), Postives = 149/151 (98.68%), Query Frame = 0


Query: 1   MGVGGTLEYLSDLVGSSHKHKKKKQLQTVELKVRMDCDGCELKVKNALSSLSGVKSVEIN 60
           MGVGGTLEYLSDLVGSSHKHKKKKQLQTVELKVRMDCDGCELKVKNALSSLSGVKSVEIN
Sbjct: 1   MGVGGTLEYLSDLVGSSHKHKKKKQLQTVELKVRMDCDGCELKVKNALSSLSGVKSVEIN 60

Query: 61  RKQQKVTVTGYVEASKVLKKAKSTGKKAEIWPYVPYSLVAQPYIAQAYDKKAPPGYVRNV 120
           RKQQKVTVTGYVEASKVLKKAKSTGKKAEIWPYVPYSLV+QPYIAQAYDKKAPPGYVRNV
Sbjct: 61  RKQQKVTVTGYVEASKVLKKAKSTGKKAEIWPYVPYSLVSQPYIAQAYDKKAPPGYVRNV 120

Query: 121 EQTAITASVTKYEDPYMNMFSDDNPNACSIM 152
           EQ A TASVTKYEDPY+NMFSDDNPNACSIM
Sbjct: 121 EQNATTASVTKYEDPYINMFSDDNPNACSIM 151

[Close]

BLAST of Carg16014 vs. NCBI nr
Match: XP_022133131.1 (heavy metal-associated isoprenylated plant protein 23 [Momordica charantia])

HSP 1 Score: 294.3 bits (752), Expect = 6.1e-76
Identity = 147/151 (97.35%), Postives = 149/151 (98.68%), Query Frame = 0


Query: 1   MGVGGTLEYLSDLVGSSHKHKKKKQLQTVELKVRMDCDGCELKVKNALSSLSGVKSVEIN 60
           MGVGGTLEYLSDLVGSSHKHKKKKQLQTVELKVRMDCDGCELKVKNALSSLSGVKSVEIN
Sbjct: 1   MGVGGTLEYLSDLVGSSHKHKKKKQLQTVELKVRMDCDGCELKVKNALSSLSGVKSVEIN 60

Query: 61  RKQQKVTVTGYVEASKVLKKAKSTGKKAEIWPYVPYSLVAQPYIAQAYDKKAPPGYVRNV 120
           RKQQKVTVTGYVEASKVLKKAKSTGKKAEIWPYVPYSLV+QPYIAQAYDKKAPPGYVRNV
Sbjct: 61  RKQQKVTVTGYVEASKVLKKAKSTGKKAEIWPYVPYSLVSQPYIAQAYDKKAPPGYVRNV 120

Query: 121 EQTAITASVTKYEDPYMNMFSDDNPNACSIM 152
           E TA TASVTKYEDPY+NMFSDDNPNACSIM
Sbjct: 121 EHTASTASVTKYEDPYINMFSDDNPNACSIM 151

[Close]

BLAST of Carg16014 vs. NCBI nr
Match: XP_008440632.1 (PREDICTED: heavy metal-associated isoprenylated plant protein 20 [Cucumis melo] >KAA0036278.1 heavy metal-associated isoprenylated plant protein 20 [Cucumis melo var. makuwa] >TYK12672.1 heavy metal-associated isoprenylated plant protein 20 [Cucumis melo var. makuwa])

HSP 1 Score: 294.3 bits (752), Expect = 6.1e-76
Identity = 146/151 (96.69%), Postives = 150/151 (99.34%), Query Frame = 0


Query: 1   MGVGGTLEYLSDLVGSSHKHKKKKQLQTVELKVRMDCDGCELKVKNALSSLSGVKSVEIN 60
           MGVGGTLEYLSDLVG++HKHKKKKQLQTVELKVRMDCDGCELKVKNALSSLSGVKSVEIN
Sbjct: 1   MGVGGTLEYLSDLVGNTHKHKKKKQLQTVELKVRMDCDGCELKVKNALSSLSGVKSVEIN 60

Query: 61  RKQQKVTVTGYVEASKVLKKAKSTGKKAEIWPYVPYSLVAQPYIAQAYDKKAPPGYVRNV 120
           RKQQKVTVTGYVEASKVLKKAKSTGKKAEIWPYVPYSLV+QPYIAQAYDKKAPPGYVRNV
Sbjct: 61  RKQQKVTVTGYVEASKVLKKAKSTGKKAEIWPYVPYSLVSQPYIAQAYDKKAPPGYVRNV 120

Query: 121 EQTAITASVTKYEDPYMNMFSDDNPNACSIM 152
           EQTA TASVTKYEDPY+NMFSDDNPNACSIM
Sbjct: 121 EQTATTASVTKYEDPYINMFSDDNPNACSIM 151

[Close]

BLAST of Carg16014 vs. ExPASy Swiss-Prot
Match: O65657 (Heavy metal-associated isoprenylated plant protein 23 OS=Arabidopsis thaliana OX=3702 GN=HIPP23 PE=1 SV=1)

HSP 1 Score: 232.3 bits (591), Expect = 3.7e-60
Identity = 119/158 (75.32%), Postives = 137/158 (86.71%), Query Frame = 0


Query: 1   MGVGGTLEYLSDLVGS--SH---KHKKKKQLQTVELKVRMDCDGCELKVKNALSSLSGVK 60
           MGVGGTLEY+S+L+G+  SH   K KKKKQ QTVELKVRMDCDGC LK+KN+LSSL GVK
Sbjct: 1   MGVGGTLEYISELIGNGGSHSYGKRKKKKQFQTVELKVRMDCDGCVLKIKNSLSSLKGVK 60

Query: 61  SVEINRKQQKVTVTGYVEASKVLKKAKSTGKKAEIWPYVPYSLVAQPYIAQAYDKKAPPG 120
           +VEIN+KQQKVTV+GY +ASKVLKKAK+TGKKAEIWPYVPY+LVAQPYIAQAYDKKAPPG
Sbjct: 61  TVEINKKQQKVTVSGYADASKVLKKAKATGKKAEIWPYVPYNLVAQPYIAQAYDKKAPPG 120

Query: 121 YVRNVEQTAITASV-TKYEDP-YMNMFSDDNPNACSIM 152
           YVR V+    T ++   Y+DP Y ++FSDDNPNACSIM
Sbjct: 121 YVRKVDPNVTTGTMAVYYDDPSYTSLFSDDNPNACSIM 158

[Close]

BLAST of Carg16014 vs. ExPASy Swiss-Prot
Match: O81464 (Heavy metal-associated isoprenylated plant protein 24 OS=Arabidopsis thaliana OX=3702 GN=HIPP24 PE=1 SV=1)

HSP 1 Score: 184.5 bits (467), Expect = 8.9e-46
Identity = 93/152 (61.18%), Postives = 119/152 (78.29%), Query Frame = 0


Query: 1   MGVGGTLEYLSDLVGSSHKHKKKKQLQTVELKV-RMDCDGCELKVKNALSSLSGVKSVEI 60
           MGV GT+EY+SDL+    K KKKKQ+QTV L+V R+DC+GCE K+K+ LS + GVKSV++
Sbjct: 1   MGVEGTMEYISDLL-KKRKRKKKKQMQTVALRVARIDCEGCERKIKHVLSGVKGVKSVDV 60

Query: 61  NRKQQKVTVTGYVEASKVLKKAKSTGKKAEIWPYVPYSLVAQPYIAQAYDKKAPPGYVRN 120
           + K QKVTVTGY++  KVL+ AKST KK E+WPYVPY++VA PYI+QAYDKKAPP  VR 
Sbjct: 61  DVKLQKVTVTGYIDPKKVLEAAKSTKKKVELWPYVPYTMVANPYISQAYDKKAPPNMVRK 120

Query: 121 VEQTAITASVTKYEDPYMNMFSDDNPNACSIM 152
           V  TA + + T  +D Y  MFSD+NPN+C+IM
Sbjct: 121 VPDTA-SVNETTVDDSYTIMFSDENPNSCAIM 150

[Close]

BLAST of Carg16014 vs. ExPASy Swiss-Prot
Match: Q9C9A3 (Heavy metal-associated isoprenylated plant protein 20 OS=Arabidopsis thaliana OX=3702 GN=HIPP20 PE=1 SV=1)

HSP 1 Score: 181.8 bits (460), Expect = 5.8e-45
Identity = 86/144 (59.72%), Postives = 111/144 (77.08%), Query Frame = 0


Query: 8   EYLSDLVGSSHKHKKKKQLQTVELKVRMDCDGCELKVKNALSSLSGVKSVEINRKQQKVT 67
           EY+SD    + K +K+K +QTV +KV+MDCDGCE +VKNA+SS+ GVKSVE+NRK  KVT
Sbjct: 9   EYISDYFRVTRKRRKRKVMQTVNIKVKMDCDGCERRVKNAVSSMKGVKSVEVNRKIHKVT 68

Query: 68  VTGYVEASKVLKKAKSTGKKAEIWPYVPYSLVAQPYIAQAYDKKAPPGYVRNVEQTAITA 127
           V+GYVE  KVLK+ + TGKKAEIWPYVPY++VA PY    YDKKAP GYVR  EQ+ +  
Sbjct: 69  VSGYVEPKKVLKRIERTGKKAEIWPYVPYNMVAYPYAVGTYDKKAPAGYVRKSEQSQLQL 128

Query: 128 SVTKYEDPYMNMFSDDNPNACSIM 152
                E+ Y+++FSD+NPNAC++M
Sbjct: 129 LPGAPENHYISLFSDENPNACTVM 152

[Close]

BLAST of Carg16014 vs. ExPASy Swiss-Prot
Match: Q93VP2 (Heavy metal-associated isoprenylated plant protein 22 OS=Arabidopsis thaliana OX=3702 GN=HIPP22 PE=1 SV=1)

HSP 1 Score: 174.9 bits (442), Expect = 7.1e-43
Identity = 88/153 (57.52%), Postives = 112/153 (73.20%), Query Frame = 0


Query: 5   GTLEYLSDLVG-----SSHKHKKKKQLQTVELKVRMDCDGCELKVKNALSSLSGVKSVEI 64
           G L YLS+        S  K KK+K +QTV +KV++DCDGCE K+KNA+SS+ G KSVE+
Sbjct: 2   GALNYLSEYFSNHFYVSIRKRKKRKVMQTVNIKVKIDCDGCERKIKNAVSSIKGAKSVEV 61

Query: 65  NRKQQKVTVTGYVEASKVLKKAKSTG-KKAEIWPYVPYSLVAQPYIAQAYDKKAPPGYVR 124
           NRK  KVTV+GYV+  KVLK  +STG KKAE+WPYVPY++VA PY A AYDK+APPG+VR
Sbjct: 62  NRKMHKVTVSGYVDPKKVLKTVQSTGKKKAELWPYVPYTMVAYPYAAGAYDKRAPPGFVR 121

Query: 125 NVEQTAITASVTKYEDPYMNMFSDDNPNACSIM 152
             EQ       T  +D  M++FSD+NPNAC++M
Sbjct: 122 KSEQAQAQPGST--DDKLMSLFSDENPNACTVM 152

[Close]

BLAST of Carg16014 vs. ExPASy Swiss-Prot
Match: Q9SZN7 (Heavy metal-associated isoprenylated plant protein 26 OS=Arabidopsis thaliana OX=3702 GN=HIPP26 PE=1 SV=1)

HSP 1 Score: 153.7 bits (387), Expect = 1.7e-36
Identity = 77/152 (50.66%), Postives = 104/152 (68.42%), Query Frame = 0


Query: 5   GTLEYLSDLVGSSHKH--KKKKQLQTVELKVRMDCDGCELKVKNALSSLSGVKSVEINRK 64
           G L+++S++   SH H  KK+KQLQTVE+KV+MDC+GCE KV+ ++  + GV SV +  K
Sbjct: 2   GVLDHVSEMFDCSHGHKIKKRKQLQTVEIKVKMDCEGCERKVRRSVEGMKGVSSVTLEPK 61

Query: 65  QQKVTVTGYVEASKVL-KKAKSTGKKAEIWPYVPYSLVAQPYIAQAYDKKAPPGYVRNVE 124
             KVTV GYV+ +KV+ + +  TGKK E+WPYVPY +VA PY A  YDKKAP GYVR V+
Sbjct: 62  AHKVTVVGYVDPNKVVARMSHRTGKKVELWPYVPYDVVAHPYAAGVYDKKAPSGYVRRVD 121

Query: 125 QTAIT--ASVTKYEDPYMNMFSDDNPNACSIM 152
              ++  A  +  E  Y   FSD+NP AC +M
Sbjct: 122 DPGVSQLARASSTEVRYTTAFSDENPAACVVM 153

[Close]

BLAST of Carg16014 vs. ExPASy TrEMBL
Match: A0A6J1KLT3 (heavy metal-associated isoprenylated plant protein 23-like OS=Cucurbita maxima OX=3661 GN=LOC111496887 PE=4 SV=1)

HSP 1 Score: 301.2 bits (770), Expect = 2.4e-78
Identity = 151/151 (100.00%), Postives = 151/151 (100.00%), Query Frame = 0


Query: 1   MGVGGTLEYLSDLVGSSHKHKKKKQLQTVELKVRMDCDGCELKVKNALSSLSGVKSVEIN 60
           MGVGGTLEYLSDLVGSSHKHKKKKQLQTVELKVRMDCDGCELKVKNALSSLSGVKSVEIN
Sbjct: 1   MGVGGTLEYLSDLVGSSHKHKKKKQLQTVELKVRMDCDGCELKVKNALSSLSGVKSVEIN 60

Query: 61  RKQQKVTVTGYVEASKVLKKAKSTGKKAEIWPYVPYSLVAQPYIAQAYDKKAPPGYVRNV 120
           RKQQKVTVTGYVEASKVLKKAKSTGKKAEIWPYVPYSLVAQPYIAQAYDKKAPPGYVRNV
Sbjct: 61  RKQQKVTVTGYVEASKVLKKAKSTGKKAEIWPYVPYSLVAQPYIAQAYDKKAPPGYVRNV 120

Query: 121 EQTAITASVTKYEDPYMNMFSDDNPNACSIM 152
           EQTAITASVTKYEDPYMNMFSDDNPNACSIM
Sbjct: 121 EQTAITASVTKYEDPYMNMFSDDNPNACSIM 151

[Close]

BLAST of Carg16014 vs. ExPASy TrEMBL
Match: A0A6J1HDC9 (heavy metal-associated isoprenylated plant protein 23-like OS=Cucurbita moschata OX=3662 GN=LOC111463128 PE=4 SV=1)

HSP 1 Score: 301.2 bits (770), Expect = 2.4e-78
Identity = 151/151 (100.00%), Postives = 151/151 (100.00%), Query Frame = 0


Query: 1   MGVGGTLEYLSDLVGSSHKHKKKKQLQTVELKVRMDCDGCELKVKNALSSLSGVKSVEIN 60
           MGVGGTLEYLSDLVGSSHKHKKKKQLQTVELKVRMDCDGCELKVKNALSSLSGVKSVEIN
Sbjct: 1   MGVGGTLEYLSDLVGSSHKHKKKKQLQTVELKVRMDCDGCELKVKNALSSLSGVKSVEIN 60

Query: 61  RKQQKVTVTGYVEASKVLKKAKSTGKKAEIWPYVPYSLVAQPYIAQAYDKKAPPGYVRNV 120
           RKQQKVTVTGYVEASKVLKKAKSTGKKAEIWPYVPYSLVAQPYIAQAYDKKAPPGYVRNV
Sbjct: 61  RKQQKVTVTGYVEASKVLKKAKSTGKKAEIWPYVPYSLVAQPYIAQAYDKKAPPGYVRNV 120

Query: 121 EQTAITASVTKYEDPYMNMFSDDNPNACSIM 152
           EQTAITASVTKYEDPYMNMFSDDNPNACSIM
Sbjct: 121 EQTAITASVTKYEDPYMNMFSDDNPNACSIM 151

[Close]

BLAST of Carg16014 vs. ExPASy TrEMBL
Match: A0A5A7T0C0 (Heavy metal-associated isoprenylated plant protein 20 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold255G002600 PE=4 SV=1)

HSP 1 Score: 294.3 bits (752), Expect = 2.9e-76
Identity = 146/151 (96.69%), Postives = 150/151 (99.34%), Query Frame = 0


Query: 1   MGVGGTLEYLSDLVGSSHKHKKKKQLQTVELKVRMDCDGCELKVKNALSSLSGVKSVEIN 60
           MGVGGTLEYLSDLVG++HKHKKKKQLQTVELKVRMDCDGCELKVKNALSSLSGVKSVEIN
Sbjct: 1   MGVGGTLEYLSDLVGNTHKHKKKKQLQTVELKVRMDCDGCELKVKNALSSLSGVKSVEIN 60

Query: 61  RKQQKVTVTGYVEASKVLKKAKSTGKKAEIWPYVPYSLVAQPYIAQAYDKKAPPGYVRNV 120
           RKQQKVTVTGYVEASKVLKKAKSTGKKAEIWPYVPYSLV+QPYIAQAYDKKAPPGYVRNV
Sbjct: 61  RKQQKVTVTGYVEASKVLKKAKSTGKKAEIWPYVPYSLVSQPYIAQAYDKKAPPGYVRNV 120

Query: 121 EQTAITASVTKYEDPYMNMFSDDNPNACSIM 152
           EQTA TASVTKYEDPY+NMFSDDNPNACSIM
Sbjct: 121 EQTATTASVTKYEDPYINMFSDDNPNACSIM 151

[Close]

BLAST of Carg16014 vs. ExPASy TrEMBL
Match: A0A6J1BU54 (heavy metal-associated isoprenylated plant protein 23 OS=Momordica charantia OX=3673 GN=LOC111005804 PE=4 SV=1)

HSP 1 Score: 294.3 bits (752), Expect = 2.9e-76
Identity = 147/151 (97.35%), Postives = 149/151 (98.68%), Query Frame = 0


Query: 1   MGVGGTLEYLSDLVGSSHKHKKKKQLQTVELKVRMDCDGCELKVKNALSSLSGVKSVEIN 60
           MGVGGTLEYLSDLVGSSHKHKKKKQLQTVELKVRMDCDGCELKVKNALSSLSGVKSVEIN
Sbjct: 1   MGVGGTLEYLSDLVGSSHKHKKKKQLQTVELKVRMDCDGCELKVKNALSSLSGVKSVEIN 60

Query: 61  RKQQKVTVTGYVEASKVLKKAKSTGKKAEIWPYVPYSLVAQPYIAQAYDKKAPPGYVRNV 120
           RKQQKVTVTGYVEASKVLKKAKSTGKKAEIWPYVPYSLV+QPYIAQAYDKKAPPGYVRNV
Sbjct: 61  RKQQKVTVTGYVEASKVLKKAKSTGKKAEIWPYVPYSLVSQPYIAQAYDKKAPPGYVRNV 120

Query: 121 EQTAITASVTKYEDPYMNMFSDDNPNACSIM 152
           E TA TASVTKYEDPY+NMFSDDNPNACSIM
Sbjct: 121 EHTASTASVTKYEDPYINMFSDDNPNACSIM 151

[Close]

BLAST of Carg16014 vs. ExPASy TrEMBL
Match: A0A1S3B1L2 (heavy metal-associated isoprenylated plant protein 20 OS=Cucumis melo OX=3656 GN=LOC103484992 PE=4 SV=1)

HSP 1 Score: 294.3 bits (752), Expect = 2.9e-76
Identity = 146/151 (96.69%), Postives = 150/151 (99.34%), Query Frame = 0


Query: 1   MGVGGTLEYLSDLVGSSHKHKKKKQLQTVELKVRMDCDGCELKVKNALSSLSGVKSVEIN 60
           MGVGGTLEYLSDLVG++HKHKKKKQLQTVELKVRMDCDGCELKVKNALSSLSGVKSVEIN
Sbjct: 1   MGVGGTLEYLSDLVGNTHKHKKKKQLQTVELKVRMDCDGCELKVKNALSSLSGVKSVEIN 60

Query: 61  RKQQKVTVTGYVEASKVLKKAKSTGKKAEIWPYVPYSLVAQPYIAQAYDKKAPPGYVRNV 120
           RKQQKVTVTGYVEASKVLKKAKSTGKKAEIWPYVPYSLV+QPYIAQAYDKKAPPGYVRNV
Sbjct: 61  RKQQKVTVTGYVEASKVLKKAKSTGKKAEIWPYVPYSLVSQPYIAQAYDKKAPPGYVRNV 120

Query: 121 EQTAITASVTKYEDPYMNMFSDDNPNACSIM 152
           EQTA TASVTKYEDPY+NMFSDDNPNACSIM
Sbjct: 121 EQTATTASVTKYEDPYINMFSDDNPNACSIM 151

[Close]

BLAST of Carg16014 vs. TAIR 10
Match: AT4G39700.1 (Heavy metal transport/detoxification superfamily protein )

HSP 1 Score: 232.3 bits (591), Expect = 2.6e-61
Identity = 119/158 (75.32%), Postives = 137/158 (86.71%), Query Frame = 0


Query: 1   MGVGGTLEYLSDLVGS--SH---KHKKKKQLQTVELKVRMDCDGCELKVKNALSSLSGVK 60
           MGVGGTLEY+S+L+G+  SH   K KKKKQ QTVELKVRMDCDGC LK+KN+LSSL GVK
Sbjct: 1   MGVGGTLEYISELIGNGGSHSYGKRKKKKQFQTVELKVRMDCDGCVLKIKNSLSSLKGVK 60

Query: 61  SVEINRKQQKVTVTGYVEASKVLKKAKSTGKKAEIWPYVPYSLVAQPYIAQAYDKKAPPG 120
           +VEIN+KQQKVTV+GY +ASKVLKKAK+TGKKAEIWPYVPY+LVAQPYIAQAYDKKAPPG
Sbjct: 61  TVEINKKQQKVTVSGYADASKVLKKAKATGKKAEIWPYVPYNLVAQPYIAQAYDKKAPPG 120

Query: 121 YVRNVEQTAITASV-TKYEDP-YMNMFSDDNPNACSIM 152
           YVR V+    T ++   Y+DP Y ++FSDDNPNACSIM
Sbjct: 121 YVRKVDPNVTTGTMAVYYDDPSYTSLFSDDNPNACSIM 158

[Close]

BLAST of Carg16014 vs. TAIR 10
Match: AT4G08570.1 (Heavy metal transport/detoxification superfamily protein )

HSP 1 Score: 184.5 bits (467), Expect = 6.3e-47
Identity = 93/152 (61.18%), Postives = 119/152 (78.29%), Query Frame = 0


Query: 1   MGVGGTLEYLSDLVGSSHKHKKKKQLQTVELKV-RMDCDGCELKVKNALSSLSGVKSVEI 60
           MGV GT+EY+SDL+    K KKKKQ+QTV L+V R+DC+GCE K+K+ LS + GVKSV++
Sbjct: 1   MGVEGTMEYISDLL-KKRKRKKKKQMQTVALRVARIDCEGCERKIKHVLSGVKGVKSVDV 60

Query: 61  NRKQQKVTVTGYVEASKVLKKAKSTGKKAEIWPYVPYSLVAQPYIAQAYDKKAPPGYVRN 120
           + K QKVTVTGY++  KVL+ AKST KK E+WPYVPY++VA PYI+QAYDKKAPP  VR 
Sbjct: 61  DVKLQKVTVTGYIDPKKVLEAAKSTKKKVELWPYVPYTMVANPYISQAYDKKAPPNMVRK 120

Query: 121 VEQTAITASVTKYEDPYMNMFSDDNPNACSIM 152
           V  TA + + T  +D Y  MFSD+NPN+C+IM
Sbjct: 121 VPDTA-SVNETTVDDSYTIMFSDENPNSCAIM 150

[Close]

BLAST of Carg16014 vs. TAIR 10
Match: AT1G71050.1 (Heavy metal transport/detoxification superfamily protein )

HSP 1 Score: 181.8 bits (460), Expect = 4.1e-46
Identity = 86/144 (59.72%), Postives = 111/144 (77.08%), Query Frame = 0


Query: 8   EYLSDLVGSSHKHKKKKQLQTVELKVRMDCDGCELKVKNALSSLSGVKSVEINRKQQKVT 67
           EY+SD    + K +K+K +QTV +KV+MDCDGCE +VKNA+SS+ GVKSVE+NRK  KVT
Sbjct: 9   EYISDYFRVTRKRRKRKVMQTVNIKVKMDCDGCERRVKNAVSSMKGVKSVEVNRKIHKVT 68

Query: 68  VTGYVEASKVLKKAKSTGKKAEIWPYVPYSLVAQPYIAQAYDKKAPPGYVRNVEQTAITA 127
           V+GYVE  KVLK+ + TGKKAEIWPYVPY++VA PY    YDKKAP GYVR  EQ+ +  
Sbjct: 69  VSGYVEPKKVLKRIERTGKKAEIWPYVPYNMVAYPYAVGTYDKKAPAGYVRKSEQSQLQL 128

Query: 128 SVTKYEDPYMNMFSDDNPNACSIM 152
                E+ Y+++FSD+NPNAC++M
Sbjct: 129 LPGAPENHYISLFSDENPNACTVM 152

[Close]

BLAST of Carg16014 vs. TAIR 10
Match: AT1G22990.1 (Heavy metal transport/detoxification superfamily protein )

HSP 1 Score: 174.9 bits (442), Expect = 5.0e-44
Identity = 88/153 (57.52%), Postives = 112/153 (73.20%), Query Frame = 0


Query: 5   GTLEYLSDLVG-----SSHKHKKKKQLQTVELKVRMDCDGCELKVKNALSSLSGVKSVEI 64
           G L YLS+        S  K KK+K +QTV +KV++DCDGCE K+KNA+SS+ G KSVE+
Sbjct: 2   GALNYLSEYFSNHFYVSIRKRKKRKVMQTVNIKVKIDCDGCERKIKNAVSSIKGAKSVEV 61

Query: 65  NRKQQKVTVTGYVEASKVLKKAKSTG-KKAEIWPYVPYSLVAQPYIAQAYDKKAPPGYVR 124
           NRK  KVTV+GYV+  KVLK  +STG KKAE+WPYVPY++VA PY A AYDK+APPG+VR
Sbjct: 62  NRKMHKVTVSGYVDPKKVLKTVQSTGKKKAELWPYVPYTMVAYPYAAGAYDKRAPPGFVR 121

Query: 125 NVEQTAITASVTKYEDPYMNMFSDDNPNACSIM 152
             EQ       T  +D  M++FSD+NPNAC++M
Sbjct: 122 KSEQAQAQPGST--DDKLMSLFSDENPNACTVM 152

[Close]

BLAST of Carg16014 vs. TAIR 10
Match: AT4G38580.1 (farnesylated protein 6 )

HSP 1 Score: 153.7 bits (387), Expect = 1.2e-37
Identity = 77/152 (50.66%), Postives = 104/152 (68.42%), Query Frame = 0


Query: 5   GTLEYLSDLVGSSHKH--KKKKQLQTVELKVRMDCDGCELKVKNALSSLSGVKSVEINRK 64
           G L+++S++   SH H  KK+KQLQTVE+KV+MDC+GCE KV+ ++  + GV SV +  K
Sbjct: 2   GVLDHVSEMFDCSHGHKIKKRKQLQTVEIKVKMDCEGCERKVRRSVEGMKGVSSVTLEPK 61

Query: 65  QQKVTVTGYVEASKVL-KKAKSTGKKAEIWPYVPYSLVAQPYIAQAYDKKAPPGYVRNVE 124
             KVTV GYV+ +KV+ + +  TGKK E+WPYVPY +VA PY A  YDKKAP GYVR V+
Sbjct: 62  AHKVTVVGYVDPNKVVARMSHRTGKKVELWPYVPYDVVAHPYAAGVYDKKAPSGYVRRVD 121

Query: 125 QTAIT--ASVTKYEDPYMNMFSDDNPNACSIM 152
              ++  A  +  E  Y   FSD+NP AC +M
Sbjct: 122 DPGVSQLARASSTEVRYTTAFSDENPAACVVM 153

The following BLAST results are available for this feature:

BLAST of Carg16014 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_022962722.1	5.0e-78	100.00	heavy metal-associated isoprenylated plant protein 23-like [Cucurbita moschata] ...	[more]
KAG6594861.1	5.0e-78	100.00	Aspartic proteinase-like protein 1, partial [Cucurbita argyrosperma subsp. soror...	[more]
XP_038882614.1	4.7e-76	97.35	heavy metal-associated isoprenylated plant protein 23 [Benincasa hispida]	[more]
XP_022133131.1	6.1e-76	97.35	heavy metal-associated isoprenylated plant protein 23 [Momordica charantia]	[more]
XP_008440632.1	6.1e-76	96.69	PREDICTED: heavy metal-associated isoprenylated plant protein 20 [Cucumis melo] ...	[more]

BLAST of Carg16014 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
O65657	3.7e-60	75.32	Heavy metal-associated isoprenylated plant protein 23 OS=Arabidopsis thaliana OX...	[more]
O81464	8.9e-46	61.18	Heavy metal-associated isoprenylated plant protein 24 OS=Arabidopsis thaliana OX...	[more]
Q9C9A3	5.8e-45	59.72	Heavy metal-associated isoprenylated plant protein 20 OS=Arabidopsis thaliana OX...	[more]
Q93VP2	7.1e-43	57.52	Heavy metal-associated isoprenylated plant protein 22 OS=Arabidopsis thaliana OX...	[more]
Q9SZN7	1.7e-36	50.66	Heavy metal-associated isoprenylated plant protein 26 OS=Arabidopsis thaliana OX...	[more]

BLAST of Carg16014 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1KLT3	2.4e-78	100.00	heavy metal-associated isoprenylated plant protein 23-like OS=Cucurbita maxima O...	[more]
A0A6J1HDC9	2.4e-78	100.00	heavy metal-associated isoprenylated plant protein 23-like OS=Cucurbita moschata...	[more]
A0A5A7T0C0	2.9e-76	96.69	Heavy metal-associated isoprenylated plant protein 20 OS=Cucumis melo var. makuw...	[more]
A0A6J1BU54	2.9e-76	97.35	heavy metal-associated isoprenylated plant protein 23 OS=Momordica charantia OX=...	[more]
A0A1S3B1L2	2.9e-76	96.69	heavy metal-associated isoprenylated plant protein 20 OS=Cucumis melo OX=3656 GN...	[more]

BLAST of Carg16014 vs. TAIR 10


Match Name	E-value	Identity	Description
AT4G39700.1	2.6e-61	75.32	Heavy metal transport/detoxification superfamily protein	[more]
AT4G08570.1	6.3e-47	61.18	Heavy metal transport/detoxification superfamily protein	[more]
AT1G71050.1	4.1e-46	59.72	Heavy metal transport/detoxification superfamily protein	[more]
AT1G22990.1	5.0e-44	57.52	Heavy metal transport/detoxification superfamily protein	[more]
AT4G38580.1	1.2e-37	50.66	farnesylated protein 6	[more]

InterPro

Analysis Name: InterPro Annotations of Silver-seed gourd (SMH-JMG-627) v2
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR006121	Heavy metal-associated domain, HMA	PFAM	PF00403	HMA	coord: 32..87 e-value: 5.7E-14 score: 52.3
IPR006121	Heavy metal-associated domain, HMA	PROSITE	PS50846	HMA_2	coord: 35..90 score: 11.852175
IPR006121	Heavy metal-associated domain, HMA	CDD	cd00371	HMA	coord: 30..89 e-value: 1.40273E-14 score: 62.2381
None	No IPR available	GENE3D	3.30.70.100		coord: 14..92 e-value: 2.9E-20 score: 74.2
None	No IPR available	PANTHER	PTHR22814	COPPER TRANSPORT PROTEIN ATOX1-RELATED	coord: 4..151
None	No IPR available	PANTHER	PTHR22814:SF325	HEAVY METAL-ASSOCIATED ISOPRENYLATED PLANT PROTEIN 20	coord: 4..151
IPR036163	Heavy metal-associated domain superfamily	SUPERFAMILY	55008	HMA, heavy metal-associated domain	coord: 26..92

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Carg16014-RA	Carg16014-RA	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
molecular_function	GO:0046872	metal ion binding