Carg16012 (gene) Silver-seed gourd (SMH-JMG-627) v2

Overview


Name	Carg16012
Type	gene
Organism	Cucurbita argyrosperma subsp. argyrosperma cv. SMH-JMG-627 (Silver-seed gourd (SMH-JMG-627) v2)
Description	L-ascorbate oxidase
Location	Carg_Chr07: 2351293 .. 2353344 (-)
RNA-Seq Expression	Carg16012
Synteny	Carg16012

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideexonCDS

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGGGTTCGCTGTTACAGAGTAGACAATACCTGCTAATTCTTCTCTGTTTCTTCGTGTTCTTATGGGAATCTCGAACTGCTGAATCAAGAACTCTGTACTACAAATGGGAAGTTAAGTCTGAGTTGAAGTCCCCTGATTGCTTCAAAAAGCTCACTTTGACCATTAATGGCCGATCTCCTGGTCCCACTATCTATGCCCATCAAGGCGATACTGTCATCGTTGAGCTCAAGAACAGCTTTGTTACTGAAAATGTTGCTATTCACTGGCATGGAATCCGACAGGTATTTTGAGTTTCATCTTGTGGGTTTGGTTTGATTTGGTTTGGTTTGATTTTTTTTTTGTTGTAATGTGTTTGGATTTTGTGTTGTAATTGAAGATTGGAACACCTTGGAGCGATGGAACAGAGGGTGTCACTCAATGTCCTGTTTTGCCTGGTGAAACATTCAAATATCAGTTTGTTGTCGACAGGGTGAGTGTTGGATTTGCTGGTTTTTGTTTTTTTTAATGAAGAATTTGGCGCTGAGTTTCGTCGAATTTGAAACGTGCAGCCAGGGACGTACTTATACCATGCGCATTACGGCATGCAAAGAGAAGATGGTCTGTATGGATCCATTGTGGTTTGGCTTCCAGAGGGTCAAACAGAGCCGTTCGCCTACGATCATGATCGGAATCTTATCCTCACCGATTGGTATCATAAAAGCAGCCATGAACACTCCGCCGGATTGGCTGCCGCCGGCTCTGCCTTCACCTGGGTCGGCGAGCCTGATGTAAACTCAATTTCCCAAATTCCCTTGTTTAATTCGAACTGATTCATTCATTTACTAATGGGTTTTTTTTTCTAATTTCTCAGTCGCTTTTGATTCAAGGAAGGGGACGGTTCAATTGCTCTCTGTTGGGAGCTTCTGCTACCTGCAACTCATCCAGCCCGGACTGTGTTCTTTATCCAATCACCGTCGTACCGGGGAGAACTTACCGGTTGAGGGTCTCAAGCCTCACTTCCCTTTCAGCTTTAAGTTTCCAAATTGAGGTAATAATCTTAATTTAATTGCTTGAAATTCTTGAGAAATGGCTGAAACAGAGTGTATTGAAAACAGGGTCACACCATGACAGTGGTTGAAGCAGATGGGCATTACGTCGAGCCATTTCAAGTTAGCAACTTGTTCATATACTCGGGCGAAACATATTCTGTGTTGATTAAAGCCGATCAAAACCCTTCTAGAAACTACTGGATTACAACCAATGTGGTCAGCCGGAACAGCACCACCCCACTTGGTTTAGCCATTTTCAATTACTACCCAAATCACCAACGTAAATTCCCTCCGACGACACCTCCGGCTGGACCTGCCTGGAATGCGGTTGCACCCCGGTTAGCTCAAAGCCACGCCATCAAAGCCCGACAGGGCTTCATCCACCCGCCGCCAAAAGCCGCTGACAAAGTGATCGTGCTACTCAACACACAAAACACCGTGAACGGACGCCGGCGGTGGTCGTTAAACAACGTGTCGTTCAACATGCCGCACACGCCTTACCTTATTGCATTAAAGAACAATCTTCTCCACACATTCAGCCAGGCGAAACCACCCACTGGATACGACTTCAAGAACTACAACATTTTTGTTCCGCCGCCCAACCCTGAAGCGACGGTGAGCGACGCCATTTACCGATTGGATTTCAATTCAACGGTGGATATTATTCTACAGAACGCCAACATGATGGCTCTAAACACCAGCGAAACTCATCCTTGGCATTTGCACGGCCACGATTTCTGGGTTTTGGGATACGGCGAAGGAAAATTCGATATGTTTAAGGATCCAAGCAAGTACAATTTGGTGAATCCGATCATGAAGAACACTGTGCCATTGCACCCATTTGGATGGACGGCTTTGAGATTTGTGGCTGACAATCCTGGCGTTTGGGCCTTCCATTGCCATATCGACGCCCATTTCTTCATGGGAATGGGTGTTGTTTTTGAGGAAGGGATCGAAAAGGTTCAGAAATTGCCTACTTCGATCATGGGGTGTGGCGAGAGCAAGAGGTTCATAAGGCCTTAA

mRNA sequence

ATGGGTTCGCTGTTACAGAGTAGACAATACCTGCTAATTCTTCTCTGTTTCTTCGTGTTCTTATGGGAATCTCGAACTGCTGAATCAAGAACTCTGTACTACAAATGGGAAGTTAAGTCTGAGTTGAAGTCCCCTGATTGCTTCAAAAAGCTCACTTTGACCATTAATGGCCGATCTCCTGGTCCCACTATCTATGCCCATCAAGGCGATACTGTCATCGTTGAGCTCAAGAACAGCTTTGTTACTGAAAATGTTGCTATTCACTGGCATGGAATCCGACAGATTGGAACACCTTGGAGCGATGGAACAGAGGGTGTCACTCAATGTCCTGTTTTGCCTGGTGAAACATTCAAATATCAGTTTGTTGTCGACAGGCCAGGGACGTACTTATACCATGCGCATTACGGCATGCAAAGAGAAGATGGTCTGTATGGATCCATTGTGGTTTGGCTTCCAGAGGGTCAAACAGAGCCGTTCGCCTACGATCATGATCGGAATCTTATCCTCACCGATTGGTATCATAAAAGCAGCCATGAACACTCCGCCGGATTGGCTGCCGCCGGCTCTGCCTTCACCTGGGTCGGCGAGCCTGATTCGCTTTTGATTCAAGGAAGGGGACGGTTCAATTGCTCTCTGTTGGGAGCTTCTGCTACCTGCAACTCATCCAGCCCGGACTGTGTTCTTTATCCAATCACCGTCGTACCGGGGAGAACTTACCGGTTGAGGGTCTCAAGCCTCACTTCCCTTTCAGCTTTAAGTTTCCAAATTGAGGGTCACACCATGACAGTGGTTGAAGCAGATGGGCATTACGTCGAGCCATTTCAAGTTAGCAACTTGTTCATATACTCGGGCGAAACATATTCTGTGTTGATTAAAGCCGATCAAAACCCTTCTAGAAACTACTGGATTACAACCAATGTGGTCAGCCGGAACAGCACCACCCCACTTGGTTTAGCCATTTTCAATTACTACCCAAATCACCAACGTAAATTCCCTCCGACGACACCTCCGGCTGGACCTGCCTGGAATGCGGTTGCACCCCGGTTAGCTCAAAGCCACGCCATCAAAGCCCGACAGGGCTTCATCCACCCGCCGCCAAAAGCCGCTGACAAAGTGATCGTGCTACTCAACACACAAAACACCGTGAACGGACGCCGGCGGTGGTCGTTAAACAACGTGTCGTTCAACATGCCGCACACGCCTTACCTTATTGCATTAAAGAACAATCTTCTCCACACATTCAGCCAGGCGAAACCACCCACTGGATACGACTTCAAGAACTACAACATTTTTGTTCCGCCGCCCAACCCTGAAGCGACGGTGAGCGACGCCATTTACCGATTGGATTTCAATTCAACGGTGGATATTATTCTACAGAACGCCAACATGATGGCTCTAAACACCAGCGAAACTCATCCTTGGCATTTGCACGGCCACGATTTCTGGGTTTTGGGATACGGCGAAGGAAAATTCGATATGTTTAAGGATCCAAGCAAGTACAATTTGGTGAATCCGATCATGAAGAACACTGTGCCATTGCACCCATTTGGATGGACGGCTTTGAGATTTGTGGCTGACAATCCTGGCGTTTGGGCCTTCCATTGCCATATCGACGCCCATTTCTTCATGGGAATGGGTGTTGTTTTTGAGGAAGGGATCGAAAAGGTTCAGAAATTGCCTACTTCGATCATGGGGTGTGGCGAGAGCAAGAGGTTCATAAGGCCTTAA

Coding sequence (CDS)

ATGGGTTCGCTGTTACAGAGTAGACAATACCTGCTAATTCTTCTCTGTTTCTTCGTGTTCTTATGGGAATCTCGAACTGCTGAATCAAGAACTCTGTACTACAAATGGGAAGTTAAGTCTGAGTTGAAGTCCCCTGATTGCTTCAAAAAGCTCACTTTGACCATTAATGGCCGATCTCCTGGTCCCACTATCTATGCCCATCAAGGCGATACTGTCATCGTTGAGCTCAAGAACAGCTTTGTTACTGAAAATGTTGCTATTCACTGGCATGGAATCCGACAGATTGGAACACCTTGGAGCGATGGAACAGAGGGTGTCACTCAATGTCCTGTTTTGCCTGGTGAAACATTCAAATATCAGTTTGTTGTCGACAGGCCAGGGACGTACTTATACCATGCGCATTACGGCATGCAAAGAGAAGATGGTCTGTATGGATCCATTGTGGTTTGGCTTCCAGAGGGTCAAACAGAGCCGTTCGCCTACGATCATGATCGGAATCTTATCCTCACCGATTGGTATCATAAAAGCAGCCATGAACACTCCGCCGGATTGGCTGCCGCCGGCTCTGCCTTCACCTGGGTCGGCGAGCCTGATTCGCTTTTGATTCAAGGAAGGGGACGGTTCAATTGCTCTCTGTTGGGAGCTTCTGCTACCTGCAACTCATCCAGCCCGGACTGTGTTCTTTATCCAATCACCGTCGTACCGGGGAGAACTTACCGGTTGAGGGTCTCAAGCCTCACTTCCCTTTCAGCTTTAAGTTTCCAAATTGAGGGTCACACCATGACAGTGGTTGAAGCAGATGGGCATTACGTCGAGCCATTTCAAGTTAGCAACTTGTTCATATACTCGGGCGAAACATATTCTGTGTTGATTAAAGCCGATCAAAACCCTTCTAGAAACTACTGGATTACAACCAATGTGGTCAGCCGGAACAGCACCACCCCACTTGGTTTAGCCATTTTCAATTACTACCCAAATCACCAACGTAAATTCCCTCCGACGACACCTCCGGCTGGACCTGCCTGGAATGCGGTTGCACCCCGGTTAGCTCAAAGCCACGCCATCAAAGCCCGACAGGGCTTCATCCACCCGCCGCCAAAAGCCGCTGACAAAGTGATCGTGCTACTCAACACACAAAACACCGTGAACGGACGCCGGCGGTGGTCGTTAAACAACGTGTCGTTCAACATGCCGCACACGCCTTACCTTATTGCATTAAAGAACAATCTTCTCCACACATTCAGCCAGGCGAAACCACCCACTGGATACGACTTCAAGAACTACAACATTTTTGTTCCGCCGCCCAACCCTGAAGCGACGGTGAGCGACGCCATTTACCGATTGGATTTCAATTCAACGGTGGATATTATTCTACAGAACGCCAACATGATGGCTCTAAACACCAGCGAAACTCATCCTTGGCATTTGCACGGCCACGATTTCTGGGTTTTGGGATACGGCGAAGGAAAATTCGATATGTTTAAGGATCCAAGCAAGTACAATTTGGTGAATCCGATCATGAAGAACACTGTGCCATTGCACCCATTTGGATGGACGGCTTTGAGATTTGTGGCTGACAATCCTGGCGTTTGGGCCTTCCATTGCCATATCGACGCCCATTTCTTCATGGGAATGGGTGTTGTTTTTGAGGAAGGGATCGAAAAGGTTCAGAAATTGCCTACTTCGATCATGGGGTGTGGCGAGAGCAAGAGGTTCATAAGGCCTTAA

Protein sequence

MGSLLQSRQYLLILLCFFVFLWESRTAESRTLYYKWEVKSELKSPDCFKKLTLTINGRSPGPTIYAHQGDTVIVELKNSFVTENVAIHWHGIRQIGTPWSDGTEGVTQCPVLPGETFKYQFVVDRPGTYLYHAHYGMQREDGLYGSIVVWLPEGQTEPFAYDHDRNLILTDWYHKSSHEHSAGLAAAGSAFTWVGEPDSLLIQGRGRFNCSLLGASATCNSSSPDCVLYPITVVPGRTYRLRVSSLTSLSALSFQIEGHTMTVVEADGHYVEPFQVSNLFIYSGETYSVLIKADQNPSRNYWITTNVVSRNSTTPLGLAIFNYYPNHQRKFPPTTPPAGPAWNAVAPRLAQSHAIKARQGFIHPPPKAADKVIVLLNTQNTVNGRRRWSLNNVSFNMPHTPYLIALKNNLLHTFSQAKPPTGYDFKNYNIFVPPPNPEATVSDAIYRLDFNSTVDIILQNANMMALNTSETHPWHLHGHDFWVLGYGEGKFDMFKDPSKYNLVNPIMKNTVPLHPFGWTALRFVADNPGVWAFHCHIDAHFFMGMGVVFEEGIEKVQKLPTSIMGCGESKRFIRP

Homology

[Close]

BLAST of Carg16012 vs. NCBI nr
Match: KAG6594863.1 (L-ascorbate oxidase, partial [Cucurbita argyrosperma subsp. sororia] >KAG7026826.1 L-ascorbate oxidase, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 1215.7 bits (3144), Expect = 0.0e+00
Identity = 575/575 (100.00%), Postives = 575/575 (100.00%), Query Frame = 0


Query: 1   MGSLLQSRQYLLILLCFFVFLWESRTAESRTLYYKWEVKSELKSPDCFKKLTLTINGRSP 60
           MGSLLQSRQYLLILLCFFVFLWESRTAESRTLYYKWEVKSELKSPDCFKKLTLTINGRSP
Sbjct: 1   MGSLLQSRQYLLILLCFFVFLWESRTAESRTLYYKWEVKSELKSPDCFKKLTLTINGRSP 60

Query: 61  GPTIYAHQGDTVIVELKNSFVTENVAIHWHGIRQIGTPWSDGTEGVTQCPVLPGETFKYQ 120
           GPTIYAHQGDTVIVELKNSFVTENVAIHWHGIRQIGTPWSDGTEGVTQCPVLPGETFKYQ
Sbjct: 61  GPTIYAHQGDTVIVELKNSFVTENVAIHWHGIRQIGTPWSDGTEGVTQCPVLPGETFKYQ 120

Query: 121 FVVDRPGTYLYHAHYGMQREDGLYGSIVVWLPEGQTEPFAYDHDRNLILTDWYHKSSHEH 180
           FVVDRPGTYLYHAHYGMQREDGLYGSIVVWLPEGQTEPFAYDHDRNLILTDWYHKSSHEH
Sbjct: 121 FVVDRPGTYLYHAHYGMQREDGLYGSIVVWLPEGQTEPFAYDHDRNLILTDWYHKSSHEH 180

Query: 181 SAGLAAAGSAFTWVGEPDSLLIQGRGRFNCSLLGASATCNSSSPDCVLYPITVVPGRTYR 240
           SAGLAAAGSAFTWVGEPDSLLIQGRGRFNCSLLGASATCNSSSPDCVLYPITVVPGRTYR
Sbjct: 181 SAGLAAAGSAFTWVGEPDSLLIQGRGRFNCSLLGASATCNSSSPDCVLYPITVVPGRTYR 240

Query: 241 LRVSSLTSLSALSFQIEGHTMTVVEADGHYVEPFQVSNLFIYSGETYSVLIKADQNPSRN 300
           LRVSSLTSLSALSFQIEGHTMTVVEADGHYVEPFQVSNLFIYSGETYSVLIKADQNPSRN
Sbjct: 241 LRVSSLTSLSALSFQIEGHTMTVVEADGHYVEPFQVSNLFIYSGETYSVLIKADQNPSRN 300

Query: 301 YWITTNVVSRNSTTPLGLAIFNYYPNHQRKFPPTTPPAGPAWNAVAPRLAQSHAIKARQG 360
           YWITTNVVSRNSTTPLGLAIFNYYPNHQRKFPPTTPPAGPAWNAVAPRLAQSHAIKARQG
Sbjct: 301 YWITTNVVSRNSTTPLGLAIFNYYPNHQRKFPPTTPPAGPAWNAVAPRLAQSHAIKARQG 360

Query: 361 FIHPPPKAADKVIVLLNTQNTVNGRRRWSLNNVSFNMPHTPYLIALKNNLLHTFSQAKPP 420
           FIHPPPKAADKVIVLLNTQNTVNGRRRWSLNNVSFNMPHTPYLIALKNNLLHTFSQAKPP
Sbjct: 361 FIHPPPKAADKVIVLLNTQNTVNGRRRWSLNNVSFNMPHTPYLIALKNNLLHTFSQAKPP 420

Query: 421 TGYDFKNYNIFVPPPNPEATVSDAIYRLDFNSTVDIILQNANMMALNTSETHPWHLHGHD 480
           TGYDFKNYNIFVPPPNPEATVSDAIYRLDFNSTVDIILQNANMMALNTSETHPWHLHGHD
Sbjct: 421 TGYDFKNYNIFVPPPNPEATVSDAIYRLDFNSTVDIILQNANMMALNTSETHPWHLHGHD 480

Query: 481 FWVLGYGEGKFDMFKDPSKYNLVNPIMKNTVPLHPFGWTALRFVADNPGVWAFHCHIDAH 540
           FWVLGYGEGKFDMFKDPSKYNLVNPIMKNTVPLHPFGWTALRFVADNPGVWAFHCHIDAH
Sbjct: 481 FWVLGYGEGKFDMFKDPSKYNLVNPIMKNTVPLHPFGWTALRFVADNPGVWAFHCHIDAH 540

Query: 541 FFMGMGVVFEEGIEKVQKLPTSIMGCGESKRFIRP 576
           FFMGMGVVFEEGIEKVQKLPTSIMGCGESKRFIRP
Sbjct: 541 FFMGMGVVFEEGIEKVQKLPTSIMGCGESKRFIRP 575

[Close]

BLAST of Carg16012 vs. NCBI nr
Match: XP_023518520.1 (L-ascorbate oxidase [Cucurbita pepo subsp. pepo])

HSP 1 Score: 1211.4 bits (3133), Expect = 0.0e+00
Identity = 573/575 (99.65%), Postives = 574/575 (99.83%), Query Frame = 0


Query: 1   MGSLLQSRQYLLILLCFFVFLWESRTAESRTLYYKWEVKSELKSPDCFKKLTLTINGRSP 60
           MGSLLQSRQYLLILLCFFVFLWESRTAESRTLYYKWEVKSELKSPDCFKKLTLTINGRSP
Sbjct: 1   MGSLLQSRQYLLILLCFFVFLWESRTAESRTLYYKWEVKSELKSPDCFKKLTLTINGRSP 60

Query: 61  GPTIYAHQGDTVIVELKNSFVTENVAIHWHGIRQIGTPWSDGTEGVTQCPVLPGETFKYQ 120
           GPTIYAHQGDTVIVELKNSFVTENVAIHWHGIRQIGTPWSDGTEGVTQCPVLPGETFKYQ
Sbjct: 61  GPTIYAHQGDTVIVELKNSFVTENVAIHWHGIRQIGTPWSDGTEGVTQCPVLPGETFKYQ 120

Query: 121 FVVDRPGTYLYHAHYGMQREDGLYGSIVVWLPEGQTEPFAYDHDRNLILTDWYHKSSHEH 180
           FVVDRPGTYLYHAHYGMQREDGLYGSIVVWLPEGQTEPFAYDHDRNLILTDWYHKSSHEH
Sbjct: 121 FVVDRPGTYLYHAHYGMQREDGLYGSIVVWLPEGQTEPFAYDHDRNLILTDWYHKSSHEH 180

Query: 181 SAGLAAAGSAFTWVGEPDSLLIQGRGRFNCSLLGASATCNSSSPDCVLYPITVVPGRTYR 240
           SAGLAAAG+AFTWVGEPDSLLIQGRGRFNCSLLGASATCNSSSPDCVLYPITVVPGRTYR
Sbjct: 181 SAGLAAAGAAFTWVGEPDSLLIQGRGRFNCSLLGASATCNSSSPDCVLYPITVVPGRTYR 240

Query: 241 LRVSSLTSLSALSFQIEGHTMTVVEADGHYVEPFQVSNLFIYSGETYSVLIKADQNPSRN 300
           LRVSSLTSLSALSFQIEGHTMTVVEADGHYVEPFQVSNLFIYSGETYSVLIKADQNPSRN
Sbjct: 241 LRVSSLTSLSALSFQIEGHTMTVVEADGHYVEPFQVSNLFIYSGETYSVLIKADQNPSRN 300

Query: 301 YWITTNVVSRNSTTPLGLAIFNYYPNHQRKFPPTTPPAGPAWNAVAPRLAQSHAIKARQG 360
           YWITTNVVSRNSTTPLGLAIFNYYPNHQRKFPPTTPPAGPAWNAVAPRLAQSHAIKARQG
Sbjct: 301 YWITTNVVSRNSTTPLGLAIFNYYPNHQRKFPPTTPPAGPAWNAVAPRLAQSHAIKARQG 360

Query: 361 FIHPPPKAADKVIVLLNTQNTVNGRRRWSLNNVSFNMPHTPYLIALKNNLLHTFSQAKPP 420
           FIHPPPKAADKVIVLLNTQNTVNGRRRWSLNNVSFNMPHTPYLIALKNNLLHTFSQAKPP
Sbjct: 361 FIHPPPKAADKVIVLLNTQNTVNGRRRWSLNNVSFNMPHTPYLIALKNNLLHTFSQAKPP 420

Query: 421 TGYDFKNYNIFVPPPNPEATVSDAIYRLDFNSTVDIILQNANMMALNTSETHPWHLHGHD 480
           TGYDFKNYNI VPPPNPEATVSDAIYRLDFNSTVDIILQNANMMALNTSETHPWHLHGHD
Sbjct: 421 TGYDFKNYNISVPPPNPEATVSDAIYRLDFNSTVDIILQNANMMALNTSETHPWHLHGHD 480

Query: 481 FWVLGYGEGKFDMFKDPSKYNLVNPIMKNTVPLHPFGWTALRFVADNPGVWAFHCHIDAH 540
           FWVLGYGEGKFDMFKDPSKYNLVNPIMKNTVPLHPFGWTALRFVADNPGVWAFHCHIDAH
Sbjct: 481 FWVLGYGEGKFDMFKDPSKYNLVNPIMKNTVPLHPFGWTALRFVADNPGVWAFHCHIDAH 540

Query: 541 FFMGMGVVFEEGIEKVQKLPTSIMGCGESKRFIRP 576
           FFMGMGVVFEEGIEKVQKLPTSIMGCGESKRFIRP
Sbjct: 541 FFMGMGVVFEEGIEKVQKLPTSIMGCGESKRFIRP 575

[Close]

BLAST of Carg16012 vs. NCBI nr
Match: XP_022962751.1 (L-ascorbate oxidase [Cucurbita moschata])

HSP 1 Score: 1203.0 bits (3111), Expect = 0.0e+00
Identity = 571/575 (99.30%), Postives = 572/575 (99.48%), Query Frame = 0


Query: 1   MGSLLQSRQYLLILLCFFVFLWESRTAESRTLYYKWEVKSELKSPDCFKKLTLTINGRSP 60
           MGSLLQSRQ LLILLCFFVFL ESRTAESRTLYYKWEVKSELKSPDCFKKLTLTINGRSP
Sbjct: 1   MGSLLQSRQNLLILLCFFVFLRESRTAESRTLYYKWEVKSELKSPDCFKKLTLTINGRSP 60

Query: 61  GPTIYAHQGDTVIVELKNSFVTENVAIHWHGIRQIGTPWSDGTEGVTQCPVLPGETFKYQ 120
           GPTIYAHQGDTVIVELKNSFVTENVAIHWHGIRQIGTPWSDGTEGVTQCPVLPGETFKYQ
Sbjct: 61  GPTIYAHQGDTVIVELKNSFVTENVAIHWHGIRQIGTPWSDGTEGVTQCPVLPGETFKYQ 120

Query: 121 FVVDRPGTYLYHAHYGMQREDGLYGSIVVWLPEGQTEPFAYDHDRNLILTDWYHKSSHEH 180
           FVVDRPGTYLYHAHYGMQREDGL+GSIVVWLPEGQTEPFAYDHDRNLILTDWYHKSSHEH
Sbjct: 121 FVVDRPGTYLYHAHYGMQREDGLFGSIVVWLPEGQTEPFAYDHDRNLILTDWYHKSSHEH 180

Query: 181 SAGLAAAGSAFTWVGEPDSLLIQGRGRFNCSLLGASATCNSSSPDCVLYPITVVPGRTYR 240
           SAGLAAAGSAFTWVGEPDSLLIQGRGRFNCS LGASATCNSSSPDCVLYPITVVPGRTYR
Sbjct: 181 SAGLAAAGSAFTWVGEPDSLLIQGRGRFNCSRLGASATCNSSSPDCVLYPITVVPGRTYR 240

Query: 241 LRVSSLTSLSALSFQIEGHTMTVVEADGHYVEPFQVSNLFIYSGETYSVLIKADQNPSRN 300
           LRVSSLTSLSALSFQIEGHTMTVVEADGHYVEPFQVSNLFIYSGETYSVLIKADQNPSRN
Sbjct: 241 LRVSSLTSLSALSFQIEGHTMTVVEADGHYVEPFQVSNLFIYSGETYSVLIKADQNPSRN 300

Query: 301 YWITTNVVSRNSTTPLGLAIFNYYPNHQRKFPPTTPPAGPAWNAVAPRLAQSHAIKARQG 360
           YWITTNVVSRNSTTPLGLAIFNYYPNHQRKFPPTTPPAGPAWNAVAPRLAQSHAIKARQG
Sbjct: 301 YWITTNVVSRNSTTPLGLAIFNYYPNHQRKFPPTTPPAGPAWNAVAPRLAQSHAIKARQG 360

Query: 361 FIHPPPKAADKVIVLLNTQNTVNGRRRWSLNNVSFNMPHTPYLIALKNNLLHTFSQAKPP 420
           FIHPPPKAADKVIVLLNTQNTVNGRRRWSLNNVSFNMPHTPYLIALKNNLLHTFSQAKPP
Sbjct: 361 FIHPPPKAADKVIVLLNTQNTVNGRRRWSLNNVSFNMPHTPYLIALKNNLLHTFSQAKPP 420

Query: 421 TGYDFKNYNIFVPPPNPEATVSDAIYRLDFNSTVDIILQNANMMALNTSETHPWHLHGHD 480
           TGYDFKNYNIFVPPPNPEATVSDAIYRLDFNSTVDIILQNANMMALNTSETHPWHLHGHD
Sbjct: 421 TGYDFKNYNIFVPPPNPEATVSDAIYRLDFNSTVDIILQNANMMALNTSETHPWHLHGHD 480

Query: 481 FWVLGYGEGKFDMFKDPSKYNLVNPIMKNTVPLHPFGWTALRFVADNPGVWAFHCHIDAH 540
           FWVLGYGEGKFDMFKDPSKYNLVNPIMKNTVPLHPFGWTALRFVADNPGVWAFHCHIDAH
Sbjct: 481 FWVLGYGEGKFDMFKDPSKYNLVNPIMKNTVPLHPFGWTALRFVADNPGVWAFHCHIDAH 540

Query: 541 FFMGMGVVFEEGIEKVQKLPTSIMGCGESKRFIRP 576
           FFMGMGVVFEEGIEKVQKLPTSIMGCGESKRFIRP
Sbjct: 541 FFMGMGVVFEEGIEKVQKLPTSIMGCGESKRFIRP 575

[Close]

BLAST of Carg16012 vs. NCBI nr
Match: XP_023003749.1 (L-ascorbate oxidase [Cucurbita maxima])

HSP 1 Score: 1199.9 bits (3103), Expect = 0.0e+00
Identity = 569/575 (98.96%), Postives = 570/575 (99.13%), Query Frame = 0


Query: 1   MGSLLQSRQYLLILLCFFVFLWESRTAESRTLYYKWEVKSELKSPDCFKKLTLTINGRSP 60
           MGSLLQ RQYLLILLCFFVFLWESRTAESRTLYY+WEVKSELKSPDCFKKLTLTINGRSP
Sbjct: 1   MGSLLQRRQYLLILLCFFVFLWESRTAESRTLYYRWEVKSELKSPDCFKKLTLTINGRSP 60

Query: 61  GPTIYAHQGDTVIVELKNSFVTENVAIHWHGIRQIGTPWSDGTEGVTQCPVLPGETFKYQ 120
           GPTIYAHQGDTVIVELKNSFVTENVAIHWHGIRQIGTPWSDGTEGVTQCPVLPGETFKYQ
Sbjct: 61  GPTIYAHQGDTVIVELKNSFVTENVAIHWHGIRQIGTPWSDGTEGVTQCPVLPGETFKYQ 120

Query: 121 FVVDRPGTYLYHAHYGMQREDGLYGSIVVWLPEGQTEPFAYDHDRNLILTDWYHKSSHEH 180
           FVVDRPGTYLYHAHYGMQREDGLYGSIVVWLPEGQTEPFAYDHDRNLILTDWYHKSSHEH
Sbjct: 121 FVVDRPGTYLYHAHYGMQREDGLYGSIVVWLPEGQTEPFAYDHDRNLILTDWYHKSSHEH 180

Query: 181 SAGLAAAGSAFTWVGEPDSLLIQGRGRFNCSLLGASATCNSSSPDCVLYPITVVPGRTYR 240
           SAGLAA GSAF WVGEPDSLLIQGRGRFNCS LGASATCNSSSPDCVLYPITVVPGRTYR
Sbjct: 181 SAGLAAPGSAFNWVGEPDSLLIQGRGRFNCS-LGASATCNSSSPDCVLYPITVVPGRTYR 240

Query: 241 LRVSSLTSLSALSFQIEGHTMTVVEADGHYVEPFQVSNLFIYSGETYSVLIKADQNPSRN 300
           LRVSSLTSLSALSFQIEGHTMTVVEADGHYVEPFQVSNLFIYSGETYSVLIKADQNPSRN
Sbjct: 241 LRVSSLTSLSALSFQIEGHTMTVVEADGHYVEPFQVSNLFIYSGETYSVLIKADQNPSRN 300

Query: 301 YWITTNVVSRNSTTPLGLAIFNYYPNHQRKFPPTTPPAGPAWNAVAPRLAQSHAIKARQG 360
           YWITTNVVSRNSTTPLGLAIFNYYPNHQRKFPPTTPPAGPAWNAVAPRLAQSHAIKARQG
Sbjct: 301 YWITTNVVSRNSTTPLGLAIFNYYPNHQRKFPPTTPPAGPAWNAVAPRLAQSHAIKARQG 360

Query: 361 FIHPPPKAADKVIVLLNTQNTVNGRRRWSLNNVSFNMPHTPYLIALKNNLLHTFSQAKPP 420
           FIHPPPKAADKVIVLLNTQNTVNGRRRWSLNNVSFNMPHTPYLIALKNNLLHTFSQAKPP
Sbjct: 361 FIHPPPKAADKVIVLLNTQNTVNGRRRWSLNNVSFNMPHTPYLIALKNNLLHTFSQAKPP 420

Query: 421 TGYDFKNYNIFVPPPNPEATVSDAIYRLDFNSTVDIILQNANMMALNTSETHPWHLHGHD 480
           TGYDFKNYNIF PPPNPEATVSDAIYRLDFNSTVDIILQNANMMALNTSETHPWHLHGHD
Sbjct: 421 TGYDFKNYNIFDPPPNPEATVSDAIYRLDFNSTVDIILQNANMMALNTSETHPWHLHGHD 480

Query: 481 FWVLGYGEGKFDMFKDPSKYNLVNPIMKNTVPLHPFGWTALRFVADNPGVWAFHCHIDAH 540
           FWVLGYGEGKFDMFKDPSKYNLVNPIMKNTVPLHPFGWTALRFVADNPGVWAFHCHIDAH
Sbjct: 481 FWVLGYGEGKFDMFKDPSKYNLVNPIMKNTVPLHPFGWTALRFVADNPGVWAFHCHIDAH 540

Query: 541 FFMGMGVVFEEGIEKVQKLPTSIMGCGESKRFIRP 576
           FFMGMGVVFEEGIEKVQKLPTSIMGCGESKRFIRP
Sbjct: 541 FFMGMGVVFEEGIEKVQKLPTSIMGCGESKRFIRP 574

[Close]

BLAST of Carg16012 vs. NCBI nr
Match: XP_038881120.1 (L-ascorbate oxidase [Benincasa hispida])

HSP 1 Score: 1114.4 bits (2881), Expect = 0.0e+00
Identity = 523/577 (90.64%), Postives = 551/577 (95.49%), Query Frame = 0


Query: 1   MGSLLQSRQYLLILLCFFVFLWESRTAESRTLYYKWEVKSELKSPDCFKKLTLTINGRSP 60
           M SLLQSRQYLLILLCF +FL +S  AESRTL YKWEVKSELKSPDCFKKLT+TINGRSP
Sbjct: 18  MASLLQSRQYLLILLCFLLFLIQSPIAESRTLNYKWEVKSELKSPDCFKKLTMTINGRSP 77

Query: 61  GPTIYAHQGDTVIVELKNSFVTENVAIHWHGIRQIGTPWSDGTEGVTQCPVLPGETFKYQ 120
           GPTIYAHQGDTVIVELKNSF TENVAIHWHGIRQIGTPWSDGTEGVTQCP+LPGETFKYQ
Sbjct: 78  GPTIYAHQGDTVIVELKNSFSTENVAIHWHGIRQIGTPWSDGTEGVTQCPILPGETFKYQ 137

Query: 121 FVVDRPGTYLYHAHYGMQREDGLYGSIVVWLPEGQTEPFAYDHDRNLILTDWYHKSSHEH 180
           FVVDRPGTYLYHAHYGMQREDGLYGSIVVWLPEGQTEPF+YD+DRNLILTDWYHK+SHEH
Sbjct: 138 FVVDRPGTYLYHAHYGMQREDGLYGSIVVWLPEGQTEPFSYDYDRNLILTDWYHKTSHEH 197

Query: 181 SAGLAAAGSAFTWVGEPDSLLIQGRGRFNCSLLGA--SATCNSSSPDCVLYPITVVPGRT 240
           +AGLA  GS FTWVGEPDSLLIQGRGRFNCS+LG+  SATCNSS+P+C+LYPITVVPG+T
Sbjct: 198 AAGLATPGSGFTWVGEPDSLLIQGRGRFNCSVLGSSTSATCNSSNPECILYPITVVPGKT 257

Query: 241 YRLRVSSLTSLSALSFQIEGHTMTVVEADGHYVEPFQVSNLFIYSGETYSVLIKADQNPS 300
           YRLRVSSLTSLSALSFQIEGHTMTVVEADGHYVEPFQV+NLFIYSGETYSVLIKADQ+PS
Sbjct: 258 YRLRVSSLTSLSALSFQIEGHTMTVVEADGHYVEPFQVNNLFIYSGETYSVLIKADQDPS 317

Query: 301 RNYWITTNVVSRNSTTPLGLAIFNYYPNHQRKFPPTTPPAGPAWNAVAPRLAQSHAIKAR 360
           RNYWITTNVVSR+ TTP GLAIFNYYPNHQRK PPTTPPA PAWN VAPRLAQSHAIKAR
Sbjct: 318 RNYWITTNVVSRSRTTPPGLAIFNYYPNHQRKLPPTTPPAPPAWNDVAPRLAQSHAIKAR 377

Query: 361 QGFIHPPPKAADKVIVLLNTQNTVNGRRRWSLNNVSFNMPHTPYLIALKNNLLHTFSQAK 420
           QGFIH PPKAADKVIVLLNTQNTVNGRRRWSLNNVSFNMPHTPYLIALK+NLLH FSQ K
Sbjct: 378 QGFIHAPPKAADKVIVLLNTQNTVNGRRRWSLNNVSFNMPHTPYLIALKHNLLHAFSQDK 437

Query: 421 PPTGYDFKNYNIFVPPPNPEATVSDAIYRLDFNSTVDIILQNANMMALNTSETHPWHLHG 480
           PPTGYDFKNY+IF P PNPEATVSDAI+RLDFNSTVDIILQNANMM+ N SETHPWHLHG
Sbjct: 438 PPTGYDFKNYDIFNPAPNPEATVSDAIFRLDFNSTVDIILQNANMMSPNNSETHPWHLHG 497

Query: 481 HDFWVLGYGEGKFDMFKDPSKYNLVNPIMKNTVPLHPFGWTALRFVADNPGVWAFHCHID 540
           HDFWVLGYGEGKFD++KDPSK+NL NPIMKNTVPLHP+GWTALRFVADNPGVWAFHCHID
Sbjct: 498 HDFWVLGYGEGKFDIYKDPSKFNLENPIMKNTVPLHPYGWTALRFVADNPGVWAFHCHID 557

Query: 541 AHFFMGMGVVFEEGIEKVQKLPTSIMGCGESKRFIRP 576
           AHFFMGMGVVF EGI++VQKLPTSIMGCGESKRF+RP
Sbjct: 558 AHFFMGMGVVFAEGIDRVQKLPTSIMGCGESKRFLRP 594

[Close]

BLAST of Carg16012 vs. ExPASy Swiss-Prot
Match: P24792 (L-ascorbate oxidase OS=Cucurbita maxima OX=3661 GN=AAO PE=1 SV=2)

HSP 1 Score: 597.4 bits (1539), Expect = 1.7e-169
Identity = 283/569 (49.74%), Postives = 381/569 (66.96%), Query Frame = 0


Query: 6   QSRQYLLILLCFF--VFLWESRTAESRTLYYKWEVKSELKSPDCFKKLTLTINGRSPGPT 65
           ++R+   ++L FF  +  +   +  S+  +YKWEV+    +PDC + + + ING+ PGPT
Sbjct: 6   KAREPNFLILFFFGLILAFGISSEGSQIRHYKWEVEYMFWAPDCNENIVMGINGQFPGPT 65

Query: 66  IYAHQGDTVIVELKNSFVTENVAIHWHGIRQIGTPWSDGTEGVTQCPVLPGETFKYQFVV 125
           I A+ GDTV+VEL N   TE V IHWHGI Q GTPW+DGT  ++QC + PGETF Y F V
Sbjct: 66  IRANAGDTVVVELINKLHTEGVVIHWHGILQRGTPWADGTASISQCAINPGETFFYNFTV 125

Query: 126 DRPGTYLYHAHYGMQREDGLYGSIVVWLPEGQTEPFAYDHDRNLILTDWYHKSSHEHSAG 185
           D PGT+ YH H GMQR  GLYGS++V  P+G+ EPF YD + NL+L+DW+H+S H+   G
Sbjct: 126 DNPGTFFYHGHLGMQRSAGLYGSLIVDPPQGKKEPFHYDGEINLLLSDWWHQSIHKQEVG 185

Query: 186 LAAAGSAFTWVGEPDSLLIQGRGRFNCSLLGASAT----CN-SSSPDCVLYPITVVPGRT 245
           L++      W+GEP ++L+ GRG+F+CS+     +    C    S  C  Y   V+P +T
Sbjct: 186 LSS--KPIRWIGEPQTILLNGRGQFDCSIAAKYDSNLEPCKLKGSEPCAPYIFHVMPKKT 245

Query: 246 YRLRVSSLTSLSALSFQIEGHTMTVVEADGHYVEPFQVSNLFIYSGETYSVLIKADQNPS 305
           YR+R++S T+L+AL+F I  H + VVEADG+YV+PF  S++ IYSGE+YSVLI  DQNPS
Sbjct: 246 YRIRIASTTALAALNFAIGNHPLLVVEADGNYVQPFYTSDIDIYSGESYSVLITTDQNPS 305

Query: 306 RNYWITTNVVSRNSTTPLGLAIFNYYPNHQRKFPPTTPPAGPAWNAVAPRLAQSHAIKAR 365
            NYW++     R+  TP GL + NY PN   K P + PP  PAW+        ++ I A 
Sbjct: 306 ENYWVSVGTRGRHPNTPPGLTLLNYLPNSVSKLPTSPPPETPAWDDFDRSKNFTYRITAA 365

Query: 366 QGFIHPPPKAADKVIVLLNTQNTVNGRRRWSLNNVSFNMPHTPYLIALKNNLLHTFSQAK 425
            G   PP K +++ I LLNTQN +NG  +W++N+VS  +P TPYL A+K NLLH F Q  
Sbjct: 366 MGSPKPPVK-SNRRIFLLNTQNVINGYVKWAINDVSLALPPTPYLGAMKFNLLHAFDQNP 425

Query: 426 PPTGYDFKNYNIFVPPPNPEATVSDAIYRLDFNSTVDIILQNANMMALNTSETHPWHLHG 485
           PP  +  ++Y+I  PP N +  + + +Y+      VD+ILQNANMM  N SE HPWHLHG
Sbjct: 426 PPEVFP-EDYDIDTPPTNEKTKIGNGVYQFKIGEIVDVILQNANMMKENLSEIHPWHLHG 485

Query: 486 HDFWVLGYGEGKFDMFKDPSKYNLVNPIMKNTVPLHPFGWTALRFVADNPGVWAFHCHID 545
           HDFWVLGYG+GKF   ++ S  NL NP ++NTV + P+GWTA+RFVADNPGVWAFHCHI+
Sbjct: 486 HDFWVLGYGDGKFTA-EEESSLNLKNPPLRNTVVIFPYGWTAIRFVADNPGVWAFHCHIE 545

Query: 546 AHFFMGMGVVFEEGIEKVQKLPTSIMGCG 568
            H  MGMGVVF EG+EKV ++PT  + CG
Sbjct: 546 PHLHMGMGVVFAEGVEKVGRIPTKALACG 569

[Close]

BLAST of Carg16012 vs. ExPASy Swiss-Prot
Match: P37064 (L-ascorbate oxidase OS=Cucurbita pepo var. melopepo OX=3665 PE=1 SV=1)

HSP 1 Score: 596.7 bits (1537), Expect = 2.9e-169
Identity = 278/544 (51.10%), Postives = 369/544 (67.83%), Query Frame = 0


Query: 29  SRTLYYKWEVKSELKSPDCFKKLTLTINGRSPGPTIYAHQGDTVIVELKNSFVTENVAIH 88
           S+  +YKWEV+    +P+C + + + ING+ PGPTI A+ GD+V+VEL N   TE V IH
Sbjct: 1   SQIRHYKWEVEYMFWAPNCNENIVMGINGQFPGPTIRANAGDSVVVELTNKLHTEGVVIH 60

Query: 89  WHGIRQIGTPWSDGTEGVTQCPVLPGETFKYQFVVDRPGTYLYHAHYGMQREDGLYGSIV 148
           WHGI Q GTPW+DGT  ++QC + PGETF Y F VD PGT+ YH H GMQR  GLYGS++
Sbjct: 61  WHGILQRGTPWADGTASISQCAINPGETFFYNFTVDNPGTFFYHGHLGMQRSAGLYGSLI 120

Query: 149 VWLPEGQTEPFAYDHDRNLILTDWYHKSSHEHSAGLAAAGSAFTWVGEPDSLLIQGRGRF 208
           V  P+G+ EPF YD + NL+L+DW+H+S H+   GL++      W+GEP ++L+ GRG+F
Sbjct: 121 VDPPQGKKEPFHYDGEINLLLSDWWHQSIHKQEVGLSS--KPIRWIGEPQTILLNGRGQF 180

Query: 209 NCSLLGASAT----CN-SSSPDCVLYPITVVPGRTYRLRVSSLTSLSALSFQIEGHTMTV 268
           +CS+     +    C    S  C  Y   V P +TYR+R++S T+L+AL+F I  H + V
Sbjct: 181 DCSIAAKYDSNLEPCKLKGSESCAPYIFHVSPKKTYRIRIASTTALAALNFAIGNHQLLV 240

Query: 269 VEADGHYVEPFQVSNLFIYSGETYSVLIKADQNPSRNYWITTNVVSRNSTTPLGLAIFNY 328
           VEADG+YV+PF  S++ IYSGE+YSVLI  DQNPS NYW++    +R+  TP GL + NY
Sbjct: 241 VEADGNYVQPFYTSDIDIYSGESYSVLITTDQNPSENYWVSVGTRARHPNTPPGLTLLNY 300

Query: 329 YPNHQRKFPPTTPPAGPAWNAVAPRLAQSHAIKARQGFIHPPPKAADKVIVLLNTQNTVN 388
            PN   K P + PP  PAW+        ++ I A  G   PP K  ++ I LLNTQN +N
Sbjct: 301 LPNSVSKLPTSPPPQTPAWDDFDRSKNFTYRITAAMGSPKPPVK-FNRRIFLLNTQNVIN 360

Query: 389 GRRRWSLNNVSFNMPHTPYLIALKNNLLHTFSQAKPPTGYDFKNYNIFVPPPNPEATVSD 448
           G  +W++N+VS  +P TPYL A+K NLLH F Q  PP  +  ++Y+I  PP N +  + +
Sbjct: 361 GYVKWAINDVSLALPPTPYLGAMKYNLLHAFDQNPPPEVFP-EDYDIDTPPTNEKTRIGN 420

Query: 449 AIYRLDFNSTVDIILQNANMMALNTSETHPWHLHGHDFWVLGYGEGKFDMFKDPSKYNLV 508
            +Y+      VD+ILQNANMM  N SETHPWHLHGHDFWVLGYG+GKF   ++ S  NL 
Sbjct: 421 GVYQFKIGEVVDVILQNANMMKENLSETHPWHLHGHDFWVLGYGDGKFSA-EEESSLNLK 480

Query: 509 NPIMKNTVPLHPFGWTALRFVADNPGVWAFHCHIDAHFFMGMGVVFEEGIEKVQKLPTSI 568
           NP ++NTV + P+GWTA+RFVADNPGVWAFHCHI+ H  MGMGVVF EG+EKV ++PT  
Sbjct: 481 NPPLRNTVVIFPYGWTAIRFVADNPGVWAFHCHIEPHLHMGMGVVFAEGVEKVGRIPTKA 539

[Close]

BLAST of Carg16012 vs. ExPASy Swiss-Prot
Match: P14133 (L-ascorbate oxidase OS=Cucumis sativus OX=3659 PE=1 SV=1)

HSP 1 Score: 590.9 bits (1522), Expect = 1.6e-167
Identity = 280/565 (49.56%), Postives = 370/565 (65.49%), Query Frame = 0


Query: 10  YLLILLCFFVFLWESRTAESRTLYYKWEVKSELKSPDCFKKLTLTINGRSPGPTIYAHQG 69
           +L++ + F   +  S     +  +YKW+V+    SPDC + + + ING  PGPTI A+ G
Sbjct: 17  FLVLSIIFGFGITLSEAGFPKIKHYKWDVEYMFWSPDCVENIVMGINGEFPGPTIRANAG 76

Query: 70  DTVIVELKNSFVTENVAIHWHGIRQIGTPWSDGTEGVTQCPVLPGETFKYQFVVDRPGTY 129
           D V+VEL N   TE V IHWHGI Q GTPW+DGT  ++QC + PGETF Y+FVVD+ GTY
Sbjct: 77  DIVVVELTNKLHTEGVVIHWHGILQRGTPWADGTASISQCAINPGETFTYRFVVDKAGTY 136

Query: 130 LYHAHYGMQREDGLYGSIVVWLPEGQTEPFAYDHDRNLILTDWYHKSSHEHSAGLAAAGS 189
            YH H GMQR  GLYGS++V  PEG++EPF YD + NL+L+DW+H+S H+   GL++   
Sbjct: 137 FYHGHLGMQRSAGLYGSLIVDPPEGRSEPFHYDEEINLLLSDWWHQSVHKQEVGLSS--K 196

Query: 190 AFTWVGEPDSLLIQGRGRFNCSLL-----GASATCNSSSPDCVLYPITVVPGRTYRLRVS 249
              W+GEP S+LI G+G+F+CS+      G      S    C  + + V P +TYR+R++
Sbjct: 197 PMRWIGEPQSILINGKGQFDCSIAAKYNQGLKQCELSGKEKCAPFILHVQPKKTYRIRIA 256

Query: 250 SLTSLSALSFQIEGHTMTVVEADGHYVEPFQVSNLFIYSGETYSVLIKADQNPSRNYWIT 309
           S T+L++L+F I  H + VVEADG+YV+PF  S++ IYSGE+YSVLI  DQNP  NYW++
Sbjct: 257 STTALASLNFAIGNHELLVVEADGNYVQPFVTSDIDIYSGESYSVLITTDQNPLENYWVS 316

Query: 310 TNVVSRNSTTPLGLAIFNYYPNHQRKFPPTTPPAGPAWNAVAPRLAQSHAIKARQGFIHP 369
             V +R   TP GL + NY PN   K P + PP  P W         +  I A  G   P
Sbjct: 317 IGVRARLPKTPPGLTLLNYLPNSASKLPISPPPETPHWEDFDRSKNFTFRIFAAMGSPKP 376

Query: 370 PPKAADKVIVLLNTQNTVNGRRRWSLNNVSFNMPHTPYLIALKNNLLHTFSQAKPPTGYD 429
           P +  ++ + LLNTQN +NG  +W++NNVS  +P TPYL A+K  L   F+Q  PP  + 
Sbjct: 377 PVR-YNRRLFLLNTQNRINGFMKWAINNVSLALPPTPYLAAMKMRLNTAFNQNPPPETFP 436

Query: 430 FKNYNIFVPPPNPEATVSDAIYRLDFNSTVDIILQNANMMALNTSETHPWHLHGHDFWVL 489
             NY+I  PPPNPE T  + +Y+ +   TVD+ILQNANM+  N SE HPWHLHGHDFWVL
Sbjct: 437 L-NYDINNPPPNPETTTGNGVYKFNMGETVDVILQNANMLNPNMSEIHPWHLHGHDFWVL 496

Query: 490 GYGEGKFDMFKDPSKYNLVNPIMKNTVPLHPFGWTALRFVADNPGVWAFHCHIDAHFFMG 549
           GYGEGKF   +D  K NL NP ++NTV + P+GWTA+RFVADNPGVWAFHCHI+ H  MG
Sbjct: 497 GYGEGKFYAPEDEKKLNLKNPPLRNTVVIFPYGWTAIRFVADNPGVWAFHCHIEPHLHMG 556

Query: 550 MGVVFEEGIEKVQKLPTSIMGCGES 570
           MGVVF EG+  V  +P   + CG +
Sbjct: 557 MGVVFAEGVHMVGMIPPKALACGST 577

[Close]

BLAST of Carg16012 vs. ExPASy Swiss-Prot
Match: Q40588 (L-ascorbate oxidase OS=Nicotiana tabacum OX=4097 GN=AAO PE=2 SV=1)

HSP 1 Score: 584.3 bits (1505), Expect = 1.5e-165
Identity = 284/567 (50.09%), Postives = 373/567 (65.78%), Query Frame = 0


Query: 10  YLLILLCFFVFLWESRTA-ESRTLYYKWEVKSELKSPDCFKKLTLTINGRSPGPTIYAHQ 69
           +  +L    + L  SR+   ++T ++KW+V+    SPD  + + + ING+ PGPTI A  
Sbjct: 8   FFFLLPLILLELSSSRSVMAAKTRHFKWDVEYIHWSPDGEESVVMGINGQFPGPTIRAKA 67

Query: 70  GDTVIVELKNSFVTENVAIHWHGIRQIGTPWSDGTEGVTQCPVLPGETFKYQFVVDRPGT 129
           GDTV V L N   TE V IHWHGIRQIGTPW+DGT  ++QC + PGETF Y+F VD+ GT
Sbjct: 68  GDTVAVHLTNKLHTEGVVIHWHGIRQIGTPWADGTAAISQCAINPGETFLYRFKVDKAGT 127

Query: 130 YLYHAHYGMQREDGLYGSIVVWLPEGQTEPFAYDHDRNLILTDWYHKSSHEHSAGLAAAG 189
           Y YH HYGMQR  GLYGS++V + EG+ EPF YD + NL+L+DW+HK SHE    L++  
Sbjct: 128 YFYHGHYGMQRSAGLYGSLIVEVGEGEKEPFHYDGEFNLLLSDWWHKGSHEQEVDLSS-- 187

Query: 190 SAFTWVGEPDSLLIQGRGRFNCSLLGASATCNSSSPDCVL--------YPITVVPGRTYR 249
           +   W+GEP +LL+ GRG++NCSL  A+       P C L          + V P + YR
Sbjct: 188 NPLRWIGEPQTLLLNGRGQYNCSL--AARFSKPPLPQCKLRGGEQYAPQILRVRPNKIYR 247

Query: 250 LRVSSLTSLSALSFQIEGHTMTVVEADGHYVEPFQVSNLFIYSGETYSVLIKADQNPSRN 309
           LRV+S T+L +LS  I GH M VVEADG+YV+PF V ++ IYSGE+YSVL K DQ+P++N
Sbjct: 248 LRVASTTALGSLSLAIGGHKMVVVEADGNYVQPFSVQDMDIYSGESYSVLFKTDQDPTKN 307

Query: 310 YWITTNVVSRNSTTPLGLAIFNYYPNHQRKFPPTTPPAGPAWNAVAPRLAQSHAIKARQG 369
           YWI+ NV  R   TP GL + NY PN   KFP   PP  P WN      + S+ I A  G
Sbjct: 308 YWISINVRGREPKTPQGLTLLNYLPNSASKFPTLPPPIAPLWNDYNHSKSFSNKIFALMG 367

Query: 370 FIHPPPKAADKVIVLLNTQNTVNGRRRWSLNNVSFNMPHTPYLIALKNNLLHTFSQAKPP 429
              PPP+   + I+LLNTQN ++G  +W++NNVS  +P   YL +++   ++ F    PP
Sbjct: 368 SPKPPPQ-NHRRIILLNTQNKIDGYTKWAINNVSLVLPTQLYLGSIRYG-INAFDTKPPP 427

Query: 430 TGYDFKNYNIFVPPPNPEATVSDAIYRLDFNSTVDIILQNANMMALNTSETHPWHLHGHD 489
             +  K+Y++    PN  +T  + +Y L FN+T+DIILQNAN +A + SE HPWHLHGHD
Sbjct: 428 DNFP-KDYDVLKQAPNSNSTYGNGVYMLKFNTTIDIILQNANALAKDVSEIHPWHLHGHD 487

Query: 490 FWVLGYGEGKFDMFKDPSKYNLVNPIMKNTVPLHPFGWTALRFVADNPGVWAFHCHIDAH 549
           FWVLGYGEGKF   KD  K+NL NP ++NT  + PFGWTALRFV DNPGVWAFHCHI+ H
Sbjct: 488 FWVLGYGEGKFSE-KDVKKFNLKNPPLRNTAVIFPFGWTALRFVTDNPGVWAFHCHIEPH 547

Query: 550 FFMGMGVVFEEGIEKVQKLPTSIMGCG 568
             MGMGV+F EG+  V+K+P   + CG
Sbjct: 548 LHMGMGVIFAEGVHLVKKIPKEALACG 566

[Close]

BLAST of Carg16012 vs. ExPASy Swiss-Prot
Match: M4DUF2 (L-ascorbate oxidase OS=Brassica rapa subsp. pekinensis OX=51351 GN=AO PE=2 SV=1)

HSP 1 Score: 578.2 bits (1489), Expect = 1.1e-163
Identity = 270/556 (48.56%), Postives = 361/556 (64.93%), Query Frame = 0


Query: 24  SRTAESRTLYYKWEVKSELKSPDCFKKLTLTINGRSPGPTIYAHQGDTVIVELKNSFVTE 83
           + TA +    Y WEV+ +   PDC + + + +NG+ PGPTI+A  GDT++V L N   TE
Sbjct: 13  AHTASAAVREYAWEVEYKFGWPDCKEGMVMAVNGQFPGPTIHALAGDTIVVHLTNKLATE 72

Query: 84  NVAIHWHGIRQIGTPWSDGTEGVTQCPVLPGETFKYQFVVDRPGTYLYHAHYGMQREDGL 143
            + IHWHGIRQ+G+PW+DG  GVTQC + PGETF Y F VD+PGT+ YH HYGMQR  GL
Sbjct: 73  GLVIHWHGIRQLGSPWADGAAGVTQCAISPGETFTYNFTVDKPGTHFYHGHYGMQRSAGL 132

Query: 144 YGSIVVWLPEGQTEPFAYDHDRNLILTDWYHKSSHEHSAGLAAAGSAFTWVGEPDSLLIQ 203
           YGS+++ + +G+ EP  YD + NL+L+DW+H+       GL++      W+GE  S+LI 
Sbjct: 133 YGSLIIDVAKGKKEPLRYDGEFNLLLSDWWHEDVLSQEIGLSS--RPMRWIGEAQSILIN 192

Query: 204 GRGRFNCSLLG-----ASATCNSSSPD-CVLYPITVVPGRTYRLRVSSLTSLSALSFQIE 263
           GRG+FNCSL       +  TC     D C    + V P +TYR+R++S T+L++L+F ++
Sbjct: 193 GRGQFNCSLAAQFSSTSLPTCTFKEGDQCAPQRLHVEPNKTYRIRLASSTALASLNFAVQ 252

Query: 264 GHTMTVVEADGHYVEPFQVSNLFIYSGETYSVLIKADQNPSRNYWITTNVVSRNSTTPLG 323
           GH + VVEADG+Y+ PF   ++ IYSGETYSVL+  DQ+PS+NY+IT  V  R   TP  
Sbjct: 253 GHKLVVVEADGNYITPFTTDDIDIYSGETYSVLLTTDQDPSQNYYITAGVRGRKPNTPPA 312

Query: 324 LAIFNYYPNHQRKFPPTTPPAGPAWNAVAPRLAQSHAIKARQGFIHPPPKAADKVIVLLN 383
           L + NY      + P + PP  P WN        S  I A  G    PP+  D+ ++LLN
Sbjct: 313 LTVLNYVTAPSSQLPTSPPPETPRWNDFDRSKNFSKKIFAAMG-SPSPPETFDERLILLN 372

Query: 384 TQNTVNGRRRWSLNNVSFNMPHTPYLIALKNNLLHTFSQAKPPTGYDFKNYNIFVPPPNP 443
           TQN + G  +W++NNVS  +P TPYL ++K NL   F+++ PP  Y   +Y+I  PP N 
Sbjct: 373 TQNLIEGFTKWAINNVSLAVPGTPYLGSVKYNLRTGFNRSSPPKDYPV-DYDIMTPPRNR 432

Query: 444 EATVSDAIYRLDFNSTVDIILQNANMMALNTSETHPWHLHGHDFWVLGYGEGKFDMFKDP 503
            A   +      FN TVD+ILQNAN +  N SE HPWHLHGHDFWVLGYGEGKF    D 
Sbjct: 433 NAKQGNVSCVFPFNVTVDVILQNANGLNANASEIHPWHLHGHDFWVLGYGEGKFKPGVDE 492

Query: 504 SKYNLVNPIMKNTVPLHPFGWTALRFVADNPGVWAFHCHIDAHFFMGMGVVFEEGIEKVQ 563
             YNL NP ++NTV L+P+GWTALRFV DNPGVW FHCHI+ H  MGMGVVF EG+ ++ 
Sbjct: 493 KTYNLKNPPLRNTVALYPYGWTALRFVTDNPGVWFFHCHIEPHLHMGMGVVFAEGLNRIG 552

Query: 564 KLPTSIMGCGESKRFI 574
           K+P   +GCG +K+F+
Sbjct: 553 KVPDEALGCGLTKQFL 564

[Close]

BLAST of Carg16012 vs. ExPASy TrEMBL
Match: A0A6J1HE32 (L-ascorbate oxidase OS=Cucurbita moschata OX=3662 GN=LOC111463148 PE=3 SV=1)

HSP 1 Score: 1203.0 bits (3111), Expect = 0.0e+00
Identity = 571/575 (99.30%), Postives = 572/575 (99.48%), Query Frame = 0


Query: 1   MGSLLQSRQYLLILLCFFVFLWESRTAESRTLYYKWEVKSELKSPDCFKKLTLTINGRSP 60
           MGSLLQSRQ LLILLCFFVFL ESRTAESRTLYYKWEVKSELKSPDCFKKLTLTINGRSP
Sbjct: 1   MGSLLQSRQNLLILLCFFVFLRESRTAESRTLYYKWEVKSELKSPDCFKKLTLTINGRSP 60

Query: 61  GPTIYAHQGDTVIVELKNSFVTENVAIHWHGIRQIGTPWSDGTEGVTQCPVLPGETFKYQ 120
           GPTIYAHQGDTVIVELKNSFVTENVAIHWHGIRQIGTPWSDGTEGVTQCPVLPGETFKYQ
Sbjct: 61  GPTIYAHQGDTVIVELKNSFVTENVAIHWHGIRQIGTPWSDGTEGVTQCPVLPGETFKYQ 120

Query: 121 FVVDRPGTYLYHAHYGMQREDGLYGSIVVWLPEGQTEPFAYDHDRNLILTDWYHKSSHEH 180
           FVVDRPGTYLYHAHYGMQREDGL+GSIVVWLPEGQTEPFAYDHDRNLILTDWYHKSSHEH
Sbjct: 121 FVVDRPGTYLYHAHYGMQREDGLFGSIVVWLPEGQTEPFAYDHDRNLILTDWYHKSSHEH 180

Query: 181 SAGLAAAGSAFTWVGEPDSLLIQGRGRFNCSLLGASATCNSSSPDCVLYPITVVPGRTYR 240
           SAGLAAAGSAFTWVGEPDSLLIQGRGRFNCS LGASATCNSSSPDCVLYPITVVPGRTYR
Sbjct: 181 SAGLAAAGSAFTWVGEPDSLLIQGRGRFNCSRLGASATCNSSSPDCVLYPITVVPGRTYR 240

Query: 241 LRVSSLTSLSALSFQIEGHTMTVVEADGHYVEPFQVSNLFIYSGETYSVLIKADQNPSRN 300
           LRVSSLTSLSALSFQIEGHTMTVVEADGHYVEPFQVSNLFIYSGETYSVLIKADQNPSRN
Sbjct: 241 LRVSSLTSLSALSFQIEGHTMTVVEADGHYVEPFQVSNLFIYSGETYSVLIKADQNPSRN 300

Query: 301 YWITTNVVSRNSTTPLGLAIFNYYPNHQRKFPPTTPPAGPAWNAVAPRLAQSHAIKARQG 360
           YWITTNVVSRNSTTPLGLAIFNYYPNHQRKFPPTTPPAGPAWNAVAPRLAQSHAIKARQG
Sbjct: 301 YWITTNVVSRNSTTPLGLAIFNYYPNHQRKFPPTTPPAGPAWNAVAPRLAQSHAIKARQG 360

Query: 361 FIHPPPKAADKVIVLLNTQNTVNGRRRWSLNNVSFNMPHTPYLIALKNNLLHTFSQAKPP 420
           FIHPPPKAADKVIVLLNTQNTVNGRRRWSLNNVSFNMPHTPYLIALKNNLLHTFSQAKPP
Sbjct: 361 FIHPPPKAADKVIVLLNTQNTVNGRRRWSLNNVSFNMPHTPYLIALKNNLLHTFSQAKPP 420

Query: 421 TGYDFKNYNIFVPPPNPEATVSDAIYRLDFNSTVDIILQNANMMALNTSETHPWHLHGHD 480
           TGYDFKNYNIFVPPPNPEATVSDAIYRLDFNSTVDIILQNANMMALNTSETHPWHLHGHD
Sbjct: 421 TGYDFKNYNIFVPPPNPEATVSDAIYRLDFNSTVDIILQNANMMALNTSETHPWHLHGHD 480

Query: 481 FWVLGYGEGKFDMFKDPSKYNLVNPIMKNTVPLHPFGWTALRFVADNPGVWAFHCHIDAH 540
           FWVLGYGEGKFDMFKDPSKYNLVNPIMKNTVPLHPFGWTALRFVADNPGVWAFHCHIDAH
Sbjct: 481 FWVLGYGEGKFDMFKDPSKYNLVNPIMKNTVPLHPFGWTALRFVADNPGVWAFHCHIDAH 540

Query: 541 FFMGMGVVFEEGIEKVQKLPTSIMGCGESKRFIRP 576
           FFMGMGVVFEEGIEKVQKLPTSIMGCGESKRFIRP
Sbjct: 541 FFMGMGVVFEEGIEKVQKLPTSIMGCGESKRFIRP 575

[Close]

BLAST of Carg16012 vs. ExPASy TrEMBL
Match: A0A6J1KU77 (L-ascorbate oxidase OS=Cucurbita maxima OX=3661 GN=LOC111497239 PE=3 SV=1)

HSP 1 Score: 1199.9 bits (3103), Expect = 0.0e+00
Identity = 569/575 (98.96%), Postives = 570/575 (99.13%), Query Frame = 0


Query: 1   MGSLLQSRQYLLILLCFFVFLWESRTAESRTLYYKWEVKSELKSPDCFKKLTLTINGRSP 60
           MGSLLQ RQYLLILLCFFVFLWESRTAESRTLYY+WEVKSELKSPDCFKKLTLTINGRSP
Sbjct: 1   MGSLLQRRQYLLILLCFFVFLWESRTAESRTLYYRWEVKSELKSPDCFKKLTLTINGRSP 60

Query: 61  GPTIYAHQGDTVIVELKNSFVTENVAIHWHGIRQIGTPWSDGTEGVTQCPVLPGETFKYQ 120
           GPTIYAHQGDTVIVELKNSFVTENVAIHWHGIRQIGTPWSDGTEGVTQCPVLPGETFKYQ
Sbjct: 61  GPTIYAHQGDTVIVELKNSFVTENVAIHWHGIRQIGTPWSDGTEGVTQCPVLPGETFKYQ 120

Query: 121 FVVDRPGTYLYHAHYGMQREDGLYGSIVVWLPEGQTEPFAYDHDRNLILTDWYHKSSHEH 180
           FVVDRPGTYLYHAHYGMQREDGLYGSIVVWLPEGQTEPFAYDHDRNLILTDWYHKSSHEH
Sbjct: 121 FVVDRPGTYLYHAHYGMQREDGLYGSIVVWLPEGQTEPFAYDHDRNLILTDWYHKSSHEH 180

Query: 181 SAGLAAAGSAFTWVGEPDSLLIQGRGRFNCSLLGASATCNSSSPDCVLYPITVVPGRTYR 240
           SAGLAA GSAF WVGEPDSLLIQGRGRFNCS LGASATCNSSSPDCVLYPITVVPGRTYR
Sbjct: 181 SAGLAAPGSAFNWVGEPDSLLIQGRGRFNCS-LGASATCNSSSPDCVLYPITVVPGRTYR 240

Query: 241 LRVSSLTSLSALSFQIEGHTMTVVEADGHYVEPFQVSNLFIYSGETYSVLIKADQNPSRN 300
           LRVSSLTSLSALSFQIEGHTMTVVEADGHYVEPFQVSNLFIYSGETYSVLIKADQNPSRN
Sbjct: 241 LRVSSLTSLSALSFQIEGHTMTVVEADGHYVEPFQVSNLFIYSGETYSVLIKADQNPSRN 300

Query: 301 YWITTNVVSRNSTTPLGLAIFNYYPNHQRKFPPTTPPAGPAWNAVAPRLAQSHAIKARQG 360
           YWITTNVVSRNSTTPLGLAIFNYYPNHQRKFPPTTPPAGPAWNAVAPRLAQSHAIKARQG
Sbjct: 301 YWITTNVVSRNSTTPLGLAIFNYYPNHQRKFPPTTPPAGPAWNAVAPRLAQSHAIKARQG 360

Query: 361 FIHPPPKAADKVIVLLNTQNTVNGRRRWSLNNVSFNMPHTPYLIALKNNLLHTFSQAKPP 420
           FIHPPPKAADKVIVLLNTQNTVNGRRRWSLNNVSFNMPHTPYLIALKNNLLHTFSQAKPP
Sbjct: 361 FIHPPPKAADKVIVLLNTQNTVNGRRRWSLNNVSFNMPHTPYLIALKNNLLHTFSQAKPP 420

Query: 421 TGYDFKNYNIFVPPPNPEATVSDAIYRLDFNSTVDIILQNANMMALNTSETHPWHLHGHD 480
           TGYDFKNYNIF PPPNPEATVSDAIYRLDFNSTVDIILQNANMMALNTSETHPWHLHGHD
Sbjct: 421 TGYDFKNYNIFDPPPNPEATVSDAIYRLDFNSTVDIILQNANMMALNTSETHPWHLHGHD 480

Query: 481 FWVLGYGEGKFDMFKDPSKYNLVNPIMKNTVPLHPFGWTALRFVADNPGVWAFHCHIDAH 540
           FWVLGYGEGKFDMFKDPSKYNLVNPIMKNTVPLHPFGWTALRFVADNPGVWAFHCHIDAH
Sbjct: 481 FWVLGYGEGKFDMFKDPSKYNLVNPIMKNTVPLHPFGWTALRFVADNPGVWAFHCHIDAH 540

Query: 541 FFMGMGVVFEEGIEKVQKLPTSIMGCGESKRFIRP 576
           FFMGMGVVFEEGIEKVQKLPTSIMGCGESKRFIRP
Sbjct: 541 FFMGMGVVFEEGIEKVQKLPTSIMGCGESKRFIRP 574

[Close]

BLAST of Carg16012 vs. ExPASy TrEMBL
Match: A0A5A7SYW4 (L-ascorbate oxidase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold255G002620 PE=3 SV=1)

HSP 1 Score: 1092.8 bits (2825), Expect = 0.0e+00
Identity = 513/576 (89.06%), Postives = 545/576 (94.62%), Query Frame = 0


Query: 1   MGSLLQSRQYLLILLCFFVFLWESRTAESRTLYYKWEVKSELKSPDCFKKLTLTINGRSP 60
           M SLLQ +QY L+LL  F+FL +S+TAESRTL YKWEVKSELKSPDCFKKLT+TINGRSP
Sbjct: 1   MASLLQRKQYFLLLLSSFLFLLQSQTAESRTLNYKWEVKSELKSPDCFKKLTITINGRSP 60

Query: 61  GPTIYAHQGDTVIVELKNSFVTENVAIHWHGIRQIGTPWSDGTEGVTQCPVLPGETFKYQ 120
           GPTIYAHQGDTVIVELKNS  TENVAIHWHGIRQIGTPWSDGTEGVTQCP+LPGETF+YQ
Sbjct: 61  GPTIYAHQGDTVIVELKNSLHTENVAIHWHGIRQIGTPWSDGTEGVTQCPILPGETFRYQ 120

Query: 121 FVVDRPGTYLYHAHYGMQREDGLYGSIVVWLPEGQTEPFAYDHDRNLILTDWYHKSSHEH 180
           FVVDRPGTYLYHAHYGMQREDGLYGSIVVW+PEGQTEPF+YDHDRNLILTDWYHKSS+EH
Sbjct: 121 FVVDRPGTYLYHAHYGMQREDGLYGSIVVWVPEGQTEPFSYDHDRNLILTDWYHKSSNEH 180

Query: 181 SAGLAAAGSAFTWVGEPDSLLIQGRGRFNCSLLGASA-TCNSSSPDCVLYPITVVPGRTY 240
           +AGLA  GS FTWVGEPDSLLIQGRGRFNCS+LG+S+ TCNSSS +CVLYP+TVVPG+TY
Sbjct: 181 AAGLATPGSGFTWVGEPDSLLIQGRGRFNCSVLGSSSGTCNSSSTECVLYPLTVVPGKTY 240

Query: 241 RLRVSSLTSLSALSFQIEGHTMTVVEADGHYVEPFQVSNLFIYSGETYSVLIKADQNPSR 300
           RLRVSSLTSLSALSFQIEGH+MTVVEADGHYVEPFQV+NLFIYSGETYSVLIKADQ+PSR
Sbjct: 241 RLRVSSLTSLSALSFQIEGHSMTVVEADGHYVEPFQVNNLFIYSGETYSVLIKADQDPSR 300

Query: 301 NYWITTNVVSRNSTTPLGLAIFNYYPNHQRKFPPTTPPAGPAWNAVAPRLAQSHAIKARQ 360
           NYWITTNVVSR+ TTP GLAIFNYYPNHQ K PPTTPPA PAWN V PRLAQS AIKARQ
Sbjct: 301 NYWITTNVVSRSRTTPPGLAIFNYYPNHQSKRPPTTPPAPPAWNDVPPRLAQSQAIKARQ 360

Query: 361 GFIHPPPKAADKVIVLLNTQNTVNGRRRWSLNNVSFNMPHTPYLIALKNNLLHTFSQAKP 420
           GFIH PPKAADKVIVLLNTQNTVNGRRRWSLNNVSFNMPHTPYLIALK+NLLHTFSQ KP
Sbjct: 361 GFIHAPPKAADKVIVLLNTQNTVNGRRRWSLNNVSFNMPHTPYLIALKHNLLHTFSQEKP 420

Query: 421 PTGYDFKNYNIFVPPPNPEATVSDAIYRLDFNSTVDIILQNANMMALNTSETHPWHLHGH 480
           PTGYDFKNY+IF P PNPEATVSDAI+RLDFNSTVD+ILQNANMM+ N SETHPWHLHGH
Sbjct: 421 PTGYDFKNYDIFNPAPNPEATVSDAIFRLDFNSTVDLILQNANMMSPNNSETHPWHLHGH 480

Query: 481 DFWVLGYGEGKFDMFKDPSKYNLVNPIMKNTVPLHPFGWTALRFVADNPGVWAFHCHIDA 540
           DFWVLGYGEGKFD++ DPSKYNL NPIMKNTVPLHP+GWTALRFVADNPGVWAFHCHIDA
Sbjct: 481 DFWVLGYGEGKFDIYNDPSKYNLENPIMKNTVPLHPYGWTALRFVADNPGVWAFHCHIDA 540

Query: 541 HFFMGMGVVFEEGIEKVQKLPTSIMGCGESKRFIRP 576
           HFFMGMGVVFEEGIE+V KLPTSIMGCGESKRF+RP
Sbjct: 541 HFFMGMGVVFEEGIERVGKLPTSIMGCGESKRFLRP 576

[Close]

BLAST of Carg16012 vs. ExPASy TrEMBL
Match: A0A1S3B151 (L-ascorbate oxidase OS=Cucumis melo OX=3656 GN=LOC103484994 PE=3 SV=1)

HSP 1 Score: 1092.8 bits (2825), Expect = 0.0e+00
Identity = 513/576 (89.06%), Postives = 545/576 (94.62%), Query Frame = 0


Query: 1   MGSLLQSRQYLLILLCFFVFLWESRTAESRTLYYKWEVKSELKSPDCFKKLTLTINGRSP 60
           M SLLQ +QY L+LL  F+FL +S+TAESRTL YKWEVKSELKSPDCFKKLT+TINGRSP
Sbjct: 1   MASLLQRKQYFLLLLSSFLFLLQSQTAESRTLNYKWEVKSELKSPDCFKKLTITINGRSP 60

Query: 61  GPTIYAHQGDTVIVELKNSFVTENVAIHWHGIRQIGTPWSDGTEGVTQCPVLPGETFKYQ 120
           GPTIYAHQGDTVIVELKNS  TENVAIHWHGIRQIGTPWSDGTEGVTQCP+LPGETF+YQ
Sbjct: 61  GPTIYAHQGDTVIVELKNSLHTENVAIHWHGIRQIGTPWSDGTEGVTQCPILPGETFRYQ 120

Query: 121 FVVDRPGTYLYHAHYGMQREDGLYGSIVVWLPEGQTEPFAYDHDRNLILTDWYHKSSHEH 180
           FVVDRPGTYLYHAHYGMQREDGLYGSIVVW+PEGQTEPF+YDHDRNLILTDWYHKSS+EH
Sbjct: 121 FVVDRPGTYLYHAHYGMQREDGLYGSIVVWVPEGQTEPFSYDHDRNLILTDWYHKSSNEH 180

Query: 181 SAGLAAAGSAFTWVGEPDSLLIQGRGRFNCSLLGASA-TCNSSSPDCVLYPITVVPGRTY 240
           +AGLA  GS FTWVGEPDSLLIQGRGRFNCS+LG+S+ TCNSSS +CVLYP+TVVPG+TY
Sbjct: 181 AAGLATPGSGFTWVGEPDSLLIQGRGRFNCSVLGSSSGTCNSSSTECVLYPLTVVPGKTY 240

Query: 241 RLRVSSLTSLSALSFQIEGHTMTVVEADGHYVEPFQVSNLFIYSGETYSVLIKADQNPSR 300
           RLRVSSLTSLSALSFQIEGH+MTVVEADGHYVEPFQV+NLFIYSGETYSVLIKADQ+PSR
Sbjct: 241 RLRVSSLTSLSALSFQIEGHSMTVVEADGHYVEPFQVNNLFIYSGETYSVLIKADQDPSR 300

Query: 301 NYWITTNVVSRNSTTPLGLAIFNYYPNHQRKFPPTTPPAGPAWNAVAPRLAQSHAIKARQ 360
           NYWITTNVVSR+ TTP GLAIFNYYPNHQ K PPTTPPA PAWN V PRLAQS AIKARQ
Sbjct: 301 NYWITTNVVSRSRTTPPGLAIFNYYPNHQSKRPPTTPPAPPAWNDVPPRLAQSQAIKARQ 360

Query: 361 GFIHPPPKAADKVIVLLNTQNTVNGRRRWSLNNVSFNMPHTPYLIALKNNLLHTFSQAKP 420
           GFIH PPKAADKVIVLLNTQNTVNGRRRWSLNNVSFNMPHTPYLIALK+NLLHTFSQ KP
Sbjct: 361 GFIHAPPKAADKVIVLLNTQNTVNGRRRWSLNNVSFNMPHTPYLIALKHNLLHTFSQEKP 420

Query: 421 PTGYDFKNYNIFVPPPNPEATVSDAIYRLDFNSTVDIILQNANMMALNTSETHPWHLHGH 480
           PTGYDFKNY+IF P PNPEATVSDAI+RLDFNSTVD+ILQNANMM+ N SETHPWHLHGH
Sbjct: 421 PTGYDFKNYDIFNPAPNPEATVSDAIFRLDFNSTVDLILQNANMMSPNNSETHPWHLHGH 480

Query: 481 DFWVLGYGEGKFDMFKDPSKYNLVNPIMKNTVPLHPFGWTALRFVADNPGVWAFHCHIDA 540
           DFWVLGYGEGKFD++ DPSKYNL NPIMKNTVPLHP+GWTALRFVADNPGVWAFHCHIDA
Sbjct: 481 DFWVLGYGEGKFDIYNDPSKYNLENPIMKNTVPLHPYGWTALRFVADNPGVWAFHCHIDA 540

Query: 541 HFFMGMGVVFEEGIEKVQKLPTSIMGCGESKRFIRP 576
           HFFMGMGVVFEEGIE+V KLPTSIMGCGESKRF+RP
Sbjct: 541 HFFMGMGVVFEEGIERVGKLPTSIMGCGESKRFLRP 576

[Close]

BLAST of Carg16012 vs. ExPASy TrEMBL
Match: A0A0A0KK62 (L-ascorbate oxidase OS=Cucumis sativus OX=3659 GN=Csa_6G500640 PE=3 SV=1)

HSP 1 Score: 1091.6 bits (2822), Expect = 0.0e+00
Identity = 514/576 (89.24%), Postives = 542/576 (94.10%), Query Frame = 0


Query: 1   MGSLLQSRQYLLILLCFFVFLWESRTAESRTLYYKWEVKSELKSPDCFKKLTLTINGRSP 60
           M S LQS +YLLILL F  FL +S+TAESRTL YKWEVKSELKSPDCFKK+TLTINGRSP
Sbjct: 1   MASPLQSTRYLLILLSFVFFLLQSQTAESRTLNYKWEVKSELKSPDCFKKVTLTINGRSP 60

Query: 61  GPTIYAHQGDTVIVELKNSFVTENVAIHWHGIRQIGTPWSDGTEGVTQCPVLPGETFKYQ 120
           GPTIYAHQGDTVIVELKNS  TENVAIHWHGIRQIGTPWSDGTEGVTQCP+LPGETF+YQ
Sbjct: 61  GPTIYAHQGDTVIVELKNSLFTENVAIHWHGIRQIGTPWSDGTEGVTQCPILPGETFRYQ 120

Query: 121 FVVDRPGTYLYHAHYGMQREDGLYGSIVVWLPEGQTEPFAYDHDRNLILTDWYHKSSHEH 180
           FVVDRPGTYLYHAHYGMQREDGLYGSIVVW+PEGQTEPF+YD DRNLILTDWYHKSSHEH
Sbjct: 121 FVVDRPGTYLYHAHYGMQREDGLYGSIVVWVPEGQTEPFSYDLDRNLILTDWYHKSSHEH 180

Query: 181 SAGLAAAGSAFTWVGEPDSLLIQGRGRFNCSLLGASA-TCNSSSPDCVLYPITVVPGRTY 240
           + GLA  GS F WVGEPDSLLIQGRGRFNCS+LG+S+ TCNSSS +CVL+P+TVVPG+TY
Sbjct: 181 ATGLATPGSGFNWVGEPDSLLIQGRGRFNCSILGSSSGTCNSSSTECVLHPLTVVPGKTY 240

Query: 241 RLRVSSLTSLSALSFQIEGHTMTVVEADGHYVEPFQVSNLFIYSGETYSVLIKADQNPSR 300
           RLRVSSLTSLSALSFQIEGHTMTVVEADGHYVEPFQV+NLFIYSGETYSVLIKADQ+PSR
Sbjct: 241 RLRVSSLTSLSALSFQIEGHTMTVVEADGHYVEPFQVNNLFIYSGETYSVLIKADQDPSR 300

Query: 301 NYWITTNVVSRNSTTPLGLAIFNYYPNHQRKFPPTTPPAGPAWNAVAPRLAQSHAIKARQ 360
           NYWITTNVVSR+ TTP GLAIFNYYPNHQ + PPTTPPA PAWN V PRLAQS AIKARQ
Sbjct: 301 NYWITTNVVSRSRTTPPGLAIFNYYPNHQSRLPPTTPPAPPAWNDVTPRLAQSQAIKARQ 360

Query: 361 GFIHPPPKAADKVIVLLNTQNTVNGRRRWSLNNVSFNMPHTPYLIALKNNLLHTFSQAKP 420
           GFIH PPKAADKVIVLLNTQNTVNGRRRWSLNNVSFNMPHTPYLIALK+NLLHTFSQ KP
Sbjct: 361 GFIHTPPKAADKVIVLLNTQNTVNGRRRWSLNNVSFNMPHTPYLIALKHNLLHTFSQEKP 420

Query: 421 PTGYDFKNYNIFVPPPNPEATVSDAIYRLDFNSTVDIILQNANMMALNTSETHPWHLHGH 480
           PTGYDFKNY+IF P PNPEATVSDAIYRLDFNSTVDIILQNANMM+ N SETHPWHLHGH
Sbjct: 421 PTGYDFKNYDIFNPAPNPEATVSDAIYRLDFNSTVDIILQNANMMSPNNSETHPWHLHGH 480

Query: 481 DFWVLGYGEGKFDMFKDPSKYNLVNPIMKNTVPLHPFGWTALRFVADNPGVWAFHCHIDA 540
           DFWVLGYGEGKFD++KDPSKYNL NPIMKNTVPLHP+GWTALRFVADNPGVWAFHCHIDA
Sbjct: 481 DFWVLGYGEGKFDLYKDPSKYNLENPIMKNTVPLHPYGWTALRFVADNPGVWAFHCHIDA 540

Query: 541 HFFMGMGVVFEEGIEKVQKLPTSIMGCGESKRFIRP 576
           HFFMGMGVVFEEGIE+V KLPTSIMGCGESKRF+RP
Sbjct: 541 HFFMGMGVVFEEGIERVGKLPTSIMGCGESKRFLRP 576

[Close]

BLAST of Carg16012 vs. TAIR 10
Match: AT4G39830.1 (Cupredoxin superfamily protein )

HSP 1 Score: 811.2 bits (2094), Expect = 5.2e-235
Identity = 381/569 (66.96%), Postives = 445/569 (78.21%), Query Frame = 0


Query: 12  LILLCFFVFLWESRTAESRTLYYKWEVKSELKSPDCFKKLTLTINGRSPGPTIYAHQGDT 71
           L++LCF    + S   + +   +KWEVK E KSPDCF+KL +TING+ PGPTI A QGDT
Sbjct: 16  LMVLCFIALFFSSVLCQGKIRRFKWEVKYEFKSPDCFEKLVITINGKFPGPTIKAQQGDT 75

Query: 72  VIVELKNSFVTENVAIHWHGIRQIGTPWSDGTEGVTQCPVLPGETFKYQFVVDRPGTYLY 131
           ++VELKNSF+TENVA+HWHGIRQIGTPW DG EGVTQCP+LPGE F YQFVVDRPGTY+Y
Sbjct: 76  IVVELKNSFMTENVAVHWHGIRQIGTPWFDGVEGVTQCPILPGEVFIYQFVVDRPGTYMY 135

Query: 132 HAHYGMQREDGLYGSIVVWLPEGQTEPFAYDHDRNLILTDWYHKSSHEHSAGLAAAGSAF 191
           H+HYGMQRE GL G I V  P  + EPF YD+DRN +LTDWYHKS  E + GLA+    F
Sbjct: 136 HSHYGMQRESGLIGMIQVSPPATEPEPFTYDYDRNFLLTDWYHKSMSEKATGLASI--PF 195

Query: 192 TWVGEPDSLLIQGRGRFNCS------LLGASATCNSSSPDCVLYPITVVPGRTYRLRVSS 251
            WVGEP SL+IQGRGRFNCS          S  CN S+ DC  + +TV+PG+TYRLR+ S
Sbjct: 196 KWVGEPQSLMIQGRGRFNCSNNLTTPPSLVSGVCNVSNADCSRFILTVIPGKTYRLRIGS 255

Query: 252 LTSLSALSFQIEGHTMTVVEADGHYVEPFQVSNLFIYSGETYSVLIKADQNPSRNYWITT 311
           LT+LSALSFQIEGH +TVVEADGHYVEPF V NLF+YSGETYSVL+KADQNP RNYWIT+
Sbjct: 256 LTALSALSFQIEGHNLTVVEADGHYVEPFTVKNLFVYSGETYSVLLKADQNPRRNYWITS 315

Query: 312 NVVSRNSTTPLGLAIFNYYPNHQRKFPPTTPPAG--PAWNAVAPRLAQSHAIKARQGFIH 371
           ++VSR +TTP   A+ NYYPNH R+ PPT+  +   P WN    RLAQS AIKAR+GFIH
Sbjct: 316 SIVSRPATTPPATAVLNYYPNHPRRRPPTSESSNIVPEWNDTRSRLAQSLAIKARRGFIH 375

Query: 372 PPPKAADKVIVLLNTQNTVNGRRRWSLNNVSFNMPHTPYLIALKNNLLHTFS-QAKPPTG 431
             P+ +DKVIVLLNTQN VNG RRWS+NNVS++ P TPYLIALK NL + F  +   P  
Sbjct: 376 ALPENSDKVIVLLNTQNEVNGYRRWSVNNVSYHHPKTPYLIALKQNLTNAFDWRFTAPEN 435

Query: 432 YDFKNYNIFVPPPNPEATVSDAIYRLDFNSTVDIILQNANMMALNTSETHPWHLHGHDFW 491
           YD +NY+IF  P N  AT SD IYRL FNSTVD+ILQNAN M  N SETHPWHLHGHDFW
Sbjct: 436 YDSRNYDIFAKPLNANATTSDGIYRLRFNSTVDVILQNANTMNANNSETHPWHLHGHDFW 495

Query: 492 VLGYGEGKFDMFKDPSKYNLVNPIMKNTVPLHPFGWTALRFVADNPGVWAFHCHIDAHFF 551
           VLGYGEGKF+  +DP +YN V+PI KNTV + PFGWTALRF ADNPGVW+FHCHI++HFF
Sbjct: 496 VLGYGEGKFNESEDPKRYNRVDPIKKNTVAVQPFGWTALRFRADNPGVWSFHCHIESHFF 555

Query: 552 MGMGVVFEEGIEKVQKLPTSIMGCGESKR 572
           MGMG+VFE GI+KV  LP+SIMGCG++KR
Sbjct: 556 MGMGIVFESGIDKVSSLPSSIMGCGQTKR 582

[Close]

BLAST of Carg16012 vs. TAIR 10
Match: AT5G21105.1 (Plant L-ascorbate oxidase )

HSP 1 Score: 568.5 bits (1464), Expect = 5.9e-162
Identity = 268/558 (48.03%), Postives = 360/558 (64.52%), Query Frame = 0


Query: 24  SRTAESRTLYYKWEVKSELKSPDCFKKLTLTINGRSPGPTIYAHQGDTVIVELKNSFVTE 83
           + TA +    Y WEV+ +  SPDC +   +T+NG  PGPTI A  GDT++V L N   TE
Sbjct: 30  THTASAAVREYHWEVEYKYWSPDCKEGAVMTVNGEFPGPTIKAFAGDTIVVNLTNKLTTE 89

Query: 84  NVAIHWHGIRQIGTPWSDGTEGVTQCPVLPGETFKYQFVVDRPGTYLYHAHYGMQREDGL 143
            + IHWHGIRQ G+PW+DG  GVTQC + PGETF Y F V++PGT+ YH HYGMQR  GL
Sbjct: 90  GLVIHWHGIRQFGSPWADGAAGVTQCAINPGETFTYNFTVEKPGTHFYHGHYGMQRSAGL 149

Query: 144 YGSIVVWLPEGQTEPFAYDHDRNLILTDWYHKSSHEHSAGLAAAGSAFTWVGEPDSLLIQ 203
           YGS++V + +G++E   YD + NL+L+DW+H++      GL++      W+GE  S+LI 
Sbjct: 150 YGSLIVDVAKGKSERLRYDGEFNLLLSDWWHEAIPSQELGLSS--KPMRWIGEAQSILIN 209

Query: 204 GRGRFNCSLLGASATCNSSSPDCVLYP--------ITVVPGRTYRLRVSSLTSLSALSFQ 263
           GRG+FNCS L A  + N+S P C            + V P +TYR+R+SS T+L++L+  
Sbjct: 210 GRGQFNCS-LAAQFSNNTSLPMCTFKEGDQCAPQILHVEPNKTYRIRLSSTTALASLNLA 269

Query: 264 IEGHTMTVVEADGHYVEPFQVSNLFIYSGETYSVLIKADQNPSRNYWITTNVVSRNSTTP 323
           ++GH + VVEADG+Y+ PF   ++ IYSGE+YSVL+  DQ+PS+NY+I+  V  R   T 
Sbjct: 270 VQGHKLVVVEADGNYITPFTTDDIDIYSGESYSVLLTTDQDPSQNYYISVGVRGRKPNTT 329

Query: 324 LGLAIFNYYPNHQRKFPPTTPPAGPAWNAVAPRLAQSHAIKARQGFIHPPPKAADKVIVL 383
             L I NY      K P + PP  P W+        S  I +  G    PPK   K ++L
Sbjct: 330 QALTILNYVTAPASKLPSSPPPVTPRWDDFERSKNFSKKIFSAMG-SPSPPKKYRKRLIL 389

Query: 384 LNTQNTVNGRRRWSLNNVSFNMPHTPYLIALKNNLLHTFSQAKPPTGYDFKNYNIFVPPP 443
           LNTQN ++G  +W++NNVS   P TPYL ++K NL   F++  PP  Y   +Y+I  PPP
Sbjct: 390 LNTQNLIDGYTKWAINNVSLVTPATPYLGSVKYNLKLGFNRKSPPRSYRM-DYDIMNPPP 449

Query: 444 NPEATVSDAIYRLDFNSTVDIILQNANMMALNTSETHPWHLHGHDFWVLGYGEGKFDMFK 503
            P  T  + IY   FN TVD+I+QNAN++    SE HPWHLHGHDFWVLGYG+GKF    
Sbjct: 450 FPNTTTGNGIYVFPFNVTVDVIIQNANVLKGIVSEIHPWHLHGHDFWVLGYGDGKFKPGI 509

Query: 504 DPSKYNLVNPIMKNTVPLHPFGWTALRFVADNPGVWAFHCHIDAHFFMGMGVVFEEGIEK 563
           D   YNL NP ++NT  L+P+GWTA+RFV DNPGVW FHCHI+ H  MGMGVVF EG+ +
Sbjct: 510 DEKTYNLKNPPLRNTAILYPYGWTAIRFVTDNPGVWFFHCHIEPHLHMGMGVVFAEGLNR 569

Query: 564 VQKLPTSIMGCGESKRFI 574
           + K+P   +GCG +K+F+
Sbjct: 570 IGKVPDEALGCGLTKQFL 582

[Close]

BLAST of Carg16012 vs. TAIR 10
Match: AT5G21100.1 (Plant L-ascorbate oxidase )

HSP 1 Score: 555.1 bits (1429), Expect = 6.8e-158
Identity = 266/557 (47.76%), Postives = 353/557 (63.38%), Query Frame = 0


Query: 26  TAESRTLYYKWEVKSELKSPDCFKKLTLTINGRSPGPTIYAHQGDTVIVELKNSFVTENV 85
           +A +  +   WEV+ +   PDC + + + ING+ PGPTI A  GDTVI+ + N   TE V
Sbjct: 18  SASAAVVESTWEVEYKYWWPDCKEGIVMAINGQFPGPTIDAVAGDTVIIHVVNKLSTEGV 77

Query: 86  AIHWHGIRQIGTPWSDGTEGVTQCPVLPGETFKYQFVVDRPGTYLYHAHYGMQREDGLYG 145
            IHWHGIRQ GTPW+DG  GVTQCP+ PGETF Y+F+VD+ GT+ YH HYGMQR  GLYG
Sbjct: 78  VIHWHGIRQKGTPWADGAAGVTQCPINPGETFTYKFIVDKAGTHFYHGHYGMQRSSGLYG 137

Query: 146 SIVVWLPEGQTEPFAYDHDRNLILTDWYHKSSHEHSAGLAAAGSAFTWVGEPDSLLIQGR 205
            ++V  P+   E   YD + NL+L+DW+H+S H     LA +     W+GEP SLLI GR
Sbjct: 138 MLIVRSPK---ERLIYDGEFNLLLSDWWHQSIHAQE--LALSSRPMRWIGEPQSLLINGR 197

Query: 206 GRFNCSLL------GASATCNSSSPD-CVLYPITVVPGRTYRLRVSSLTSLSALSFQIEG 265
           G+FNCS        G    C     D C    + V P R YRLR++S T+L++L+  ++G
Sbjct: 198 GQFNCSQAAYFNKGGEKDVCTFKENDQCAPQTLRVEPNRVYRLRIASTTALASLNLAVQG 257

Query: 266 HTMTVVEADGHYVEPFQVSNLFIYSGETYSVLIKADQNPSRNYWITTNVVSRNSTTPLGL 325
           H + VVEADG+YV PF V+++ +YSGETYSVL+K +  PS+ YWI+  V  R   TP  L
Sbjct: 258 HQLVVVEADGNYVAPFTVNDIDVYSGETYSVLLKTNALPSKKYWISVGVRGREPKTPQAL 317

Query: 326 AIFNYYPNHQRKFPPTTPPAGPAWNAVAPRLAQSHAIKARQGFIHPPPKAADKVIVLLNT 385
            + NY    + + P   PP  P WN      + S  I A +G+  PP K+ D+ ++LLNT
Sbjct: 318 TVINYVDATESR-PSHPPPVTPIWNDTDRSKSFSKKIFAAKGYPKPPEKSHDQ-LILLNT 377

Query: 386 QNTVNGRRRWSLNNVSFNMPHTPYLIALKNNLLHTFSQAKPPTGYDFKNYNIFVPPPNPE 445
           QN      +WS+NNVS ++P TPYL +++  L   +    P       NY+I  PPPNP 
Sbjct: 378 QNLYEDYTKWSINNVSLSVPVTPYLGSIRYGLKSAYDLKSPAKKLIMDNYDIMKPPPNPN 437

Query: 446 ATVSDAIYRLDFNSTVDIILQNANMMALNTSETHPWHLHGHDFWVLGYGEGKFDMFKDPS 505
            T    IY   F   VD+ILQNAN++    SE HPWH+HGHDFWVLGYGEGKF    D  
Sbjct: 438 TTKGSGIYNFAFGIVVDVILQNANVLKGVISEIHPWHIHGHDFWVLGYGEGKFKPGIDEK 497

Query: 506 KYNLVNPIMKNTVPLHPFGWTALRFVADNPGVWAFHCHIDAHFFMGMGVVFEEGIEKVQK 565
            +NL NP ++NTV L+PFGWTA+RFV DNPGVW FHCHI+ H  MGMGVVF EG++++ K
Sbjct: 498 TFNLKNPPLRNTVVLYPFGWTAIRFVTDNPGVWFFHCHIEPHLHMGMGVVFVEGVDRIGK 557

Query: 566 --LPTSIMGCGESKRFI 574
             +P   +GCG +++++
Sbjct: 558 MEIPDEALGCGLTRKWL 567

[Close]

BLAST of Carg16012 vs. TAIR 10
Match: AT5G21105.3 (Plant L-ascorbate oxidase )

HSP 1 Score: 508.1 bits (1307), Expect = 9.5e-144
Identity = 244/514 (47.47%), Postives = 327/514 (63.62%), Query Frame = 0


Query: 24  SRTAESRTLYYKWEVKSELKSPDCFKKLTLTINGRSPGPTIYAHQGDTVIVELKNSFVTE 83
           + TA +    Y WEV+ +  SPDC +   +T+NG  PGPTI A  GDT++V L N   TE
Sbjct: 30  THTASAAVREYHWEVEYKYWSPDCKEGAVMTVNGEFPGPTIKAFAGDTIVVNLTNKLTTE 89

Query: 84  NVAIHWHGIRQIGTPWSDGTEGVTQCPVLPGETFKYQFVVDRPGTYLYHAHYGMQREDGL 143
            + IHWHGIRQ G+PW+DG  GVTQC + PGETF Y F V++PGT+ YH HYGMQR  GL
Sbjct: 90  GLVIHWHGIRQFGSPWADGAAGVTQCAINPGETFTYNFTVEKPGTHFYHGHYGMQRSAGL 149

Query: 144 YGSIVVWLPEGQTEPFAYDHDRNLILTDWYHKSSHEHSAGLAAAGSAFTWVGEPDSLLIQ 203
           YGS++V + +G++E   YD + NL+L+DW+H++      GL++      W+GE  S+LI 
Sbjct: 150 YGSLIVDVAKGKSERLRYDGEFNLLLSDWWHEAIPSQELGLSS--KPMRWIGEAQSILIN 209

Query: 204 GRGRFNCSLLGASATCNSSSPDCVLYP--------ITVVPGRTYRLRVSSLTSLSALSFQ 263
           GRG+FNCS L A  + N+S P C            + V P +TYR+R+SS T+L++L+  
Sbjct: 210 GRGQFNCS-LAAQFSNNTSLPMCTFKEGDQCAPQILHVEPNKTYRIRLSSTTALASLNLA 269

Query: 264 IEGHTMTVVEADGHYVEPFQVSNLFIYSGETYSVLIKADQNPSRNYWITTNVVSRNSTTP 323
           ++GH + VVEADG+Y+ PF   ++ IYSGE+YSVL+  DQ+PS+NY+I+  V  R   T 
Sbjct: 270 VQGHKLVVVEADGNYITPFTTDDIDIYSGESYSVLLTTDQDPSQNYYISVGVRGRKPNTT 329

Query: 324 LGLAIFNYYPNHQRKFPPTTPPAGPAWNAVAPRLAQSHAIKARQGFIHPPPKAADKVIVL 383
             L I NY      K P + PP  P W+        S  I +  G    PPK   K ++L
Sbjct: 330 QALTILNYVTAPASKLPSSPPPVTPRWDDFERSKNFSKKIFSAMG-SPSPPKKYRKRLIL 389

Query: 384 LNTQNTVNGRRRWSLNNVSFNMPHTPYLIALKNNLLHTFSQAKPPTGYDFKNYNIFVPPP 443
           LNTQN ++G  +W++NNVS   P TPYL ++K NL   F++  PP  Y   +Y+I  PPP
Sbjct: 390 LNTQNLIDGYTKWAINNVSLVTPATPYLGSVKYNLKLGFNRKSPPRSYRM-DYDIMNPPP 449

Query: 444 NPEATVSDAIYRLDFNSTVDIILQNANMMALNTSETHPWHLHGHDFWVLGYGEGKFDMFK 503
            P  T  + IY   FN TVD+I+QNAN++    SE HPWHLHGHDFWVLGYG+GKF    
Sbjct: 450 FPNTTTGNGIYVFPFNVTVDVIIQNANVLKGIVSEIHPWHLHGHDFWVLGYGDGKFKPGI 509

Query: 504 DPSKYNLVNPIMKNTVPLHPFGWTALRFVADNPG 530
           D   YNL NP ++NT  L+P+GWTA+RFV DNPG
Sbjct: 510 DEKTYNLKNPPLRNTAILYPYGWTAIRFVTDNPG 538

[Close]

BLAST of Carg16012 vs. TAIR 10
Match: AT5G21105.2 (Plant L-ascorbate oxidase )

HSP 1 Score: 370.9 bits (951), Expect = 1.8e-102
Identity = 183/383 (47.78%), Postives = 244/383 (63.71%), Query Frame = 0


Query: 199 SLLIQGRGRFNCSLLGASATCNSSSPDCVLYP--------ITVVPGRTYRLRVSSLTSLS 258
           S+LI GRG+FNCS L A  + N+S P C            + V P +TYR+R+SS T+L+
Sbjct: 12  SILINGRGQFNCS-LAAQFSNNTSLPMCTFKEGDQCAPQILHVEPNKTYRIRLSSTTALA 71

Query: 259 ALSFQIEGHTMTVVEADGHYVEPFQVSNLFIYSGETYSVLIKADQNPSRNYWITTNVVSR 318
           +L+  ++GH + VVEADG+Y+ PF   ++ IYSGE+YSVL+  DQ+PS+NY+I+  V  R
Sbjct: 72  SLNLAVQGHKLVVVEADGNYITPFTTDDIDIYSGESYSVLLTTDQDPSQNYYISVGVRGR 131

Query: 319 NSTTPLGLAIFNYYPNHQRKFPPTTPPAGPAWNAVAPRLAQSHAIKARQGFIHPPPKAAD 378
              T   L I NY      K P + PP  P W+        S  I +  G    PPK   
Sbjct: 132 KPNTTQALTILNYVTAPASKLPSSPPPVTPRWDDFERSKNFSKKIFSAMG-SPSPPKKYR 191

Query: 379 KVIVLLNTQNTVNGRRRWSLNNVSFNMPHTPYLIALKNNLLHTFSQAKPPTGYDFKNYNI 438
           K ++LLNTQN ++G  +W++NNVS   P TPYL ++K NL   F++  PP  Y   +Y+I
Sbjct: 192 KRLILLNTQNLIDGYTKWAINNVSLVTPATPYLGSVKYNLKLGFNRKSPPRSYRM-DYDI 251

Query: 439 FVPPPNPEATVSDAIYRLDFNSTVDIILQNANMMALNTSETHPWHLHGHDFWVLGYGEGK 498
             PPP P  T  + IY   FN TVD+I+QNAN++    SE HPWHLHGHDFWVLGYG+GK
Sbjct: 252 MNPPPFPNTTTGNGIYVFPFNVTVDVIIQNANVLKGIVSEIHPWHLHGHDFWVLGYGDGK 311

Query: 499 FDMFKDPSKYNLVNPIMKNTVPLHPFGWTALRFVADNPGVWAFHCHIDAHFFMGMGVVFE 558
           F    D   YNL NP ++NT  L+P+GWTA+RFV DNPGVW FHCHI+ H  MGMGVVF 
Sbjct: 312 FKPGIDEKTYNLKNPPLRNTAILYPYGWTAIRFVTDNPGVWFFHCHIEPHLHMGMGVVFA 371

Query: 559 EGIEKVQKLPTSIMGCGESKRFI 574
           EG+ ++ K+P   +GCG +K+F+
Sbjct: 372 EGLNRIGKVPDEALGCGLTKQFL 391

The following BLAST results are available for this feature:

BLAST of Carg16012 vs. NCBI nr


Match Name	E-value	Identity	Description
KAG6594863.1	0.0e+00	100.00	L-ascorbate oxidase, partial [Cucurbita argyrosperma subsp. sororia] >KAG7026826...	[more]
XP_023518520.1	0.0e+00	99.65	L-ascorbate oxidase [Cucurbita pepo subsp. pepo]	[more]
XP_022962751.1	0.0e+00	99.30	L-ascorbate oxidase [Cucurbita moschata]	[more]
XP_023003749.1	0.0e+00	98.96	L-ascorbate oxidase [Cucurbita maxima]	[more]
XP_038881120.1	0.0e+00	90.64	L-ascorbate oxidase [Benincasa hispida]	[more]

BLAST of Carg16012 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
P24792	1.7e-169	49.74	L-ascorbate oxidase OS=Cucurbita maxima OX=3661 GN=AAO PE=1 SV=2	[more]
P37064	2.9e-169	51.10	L-ascorbate oxidase OS=Cucurbita pepo var. melopepo OX=3665 PE=1 SV=1	[more]
P14133	1.6e-167	49.56	L-ascorbate oxidase OS=Cucumis sativus OX=3659 PE=1 SV=1	[more]
Q40588	1.5e-165	50.09	L-ascorbate oxidase OS=Nicotiana tabacum OX=4097 GN=AAO PE=2 SV=1	[more]
M4DUF2	1.1e-163	48.56	L-ascorbate oxidase OS=Brassica rapa subsp. pekinensis OX=51351 GN=AO PE=2 SV=1	[more]

BLAST of Carg16012 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1HE32	0.0e+00	99.30	L-ascorbate oxidase OS=Cucurbita moschata OX=3662 GN=LOC111463148 PE=3 SV=1	[more]
A0A6J1KU77	0.0e+00	98.96	L-ascorbate oxidase OS=Cucurbita maxima OX=3661 GN=LOC111497239 PE=3 SV=1	[more]
A0A5A7SYW4	0.0e+00	89.06	L-ascorbate oxidase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold255G...	[more]
A0A1S3B151	0.0e+00	89.06	L-ascorbate oxidase OS=Cucumis melo OX=3656 GN=LOC103484994 PE=3 SV=1	[more]
A0A0A0KK62	0.0e+00	89.24	L-ascorbate oxidase OS=Cucumis sativus OX=3659 GN=Csa_6G500640 PE=3 SV=1	[more]

BLAST of Carg16012 vs. TAIR 10


Match Name	E-value	Identity	Description
AT4G39830.1	5.2e-235	66.96	Cupredoxin superfamily protein	[more]
AT5G21105.1	5.9e-162	48.03	Plant L-ascorbate oxidase	[more]
AT5G21100.1	6.8e-158	47.76	Plant L-ascorbate oxidase	[more]
AT5G21105.3	9.5e-144	47.47	Plant L-ascorbate oxidase	[more]
AT5G21105.2	1.8e-102	47.78	Plant L-ascorbate oxidase	[more]

InterPro

Analysis Name: InterPro Annotations of Silver-seed gourd (SMH-JMG-627) v2
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR001117	Multicopper oxidase, type 1	PFAM	PF00394	Cu-oxidase	coord: 167..325 e-value: 1.5E-46 score: 158.4
IPR017760	L-ascorbate oxidase, plants	TIGRFAM	TIGR03388	TIGR03388	coord: 33..568 e-value: 6.7E-277 score: 917.4
IPR008972	Cupredoxin	GENE3D	2.60.40.420		coord: 159..361 e-value: 4.8E-60 score: 205.1
IPR008972	Cupredoxin	GENE3D	2.60.40.420		coord: 363..573 e-value: 6.3E-70 score: 237.1
IPR008972	Cupredoxin	GENE3D	2.60.40.420		coord: 20..152 e-value: 5.7E-46 score: 158.4
IPR008972	Cupredoxin	SUPERFAMILY	49503	Cupredoxins	coord: 158..345
IPR008972	Cupredoxin	SUPERFAMILY	49503	Cupredoxins	coord: 366..568
IPR008972	Cupredoxin	SUPERFAMILY	49503	Cupredoxins	coord: 27..178
IPR011707	Multicopper oxidase, N-termianl	PFAM	PF07732	Cu-oxidase_3	coord: 41..150 e-value: 3.5E-37 score: 127.0
IPR011706	Multicopper oxidase, C-terminal	PFAM	PF07731	Cu-oxidase_2	coord: 431..552 e-value: 1.7E-36 score: 124.9
IPR045087	Multicopper oxidase	PANTHER	PTHR11709	MULTI-COPPER OXIDASE	coord: 8..570
None	No IPR available	PANTHER	PTHR11709:SF237	CUPREDOXIN SUPERFAMILY PROTEIN	coord: 8..570
IPR033138	Multicopper oxidases, conserved site	PROSITE	PS00079	MULTICOPPER_OXIDASE1	coord: 529..549
IPR002355	Multicopper oxidase, copper-binding site	PROSITE	PS00080	MULTICOPPER_OXIDASE2	coord: 534..545
IPR034267	Ascorbate oxidase, third cupredoxin domain	CDD	cd13893	CuRO_3_AAO	coord: 369..560 e-value: 1.99728E-83 score: 255.422

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Carg16012-RA	Carg16012-RA	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:1900367	positive regulation of defense response to insect
cellular_component	GO:0005576	extracellular region
cellular_component	GO:0016021	integral component of membrane
molecular_function	GO:0005507	copper ion binding
molecular_function	GO:0008447	L-ascorbate oxidase activity
molecular_function	GO:0016491	oxidoreductase activity