Carg15981 (gene) Silver-seed gourd (SMH-JMG-627) v2

BLAST of Carg15981 vs. NCBI nr
Match: XP_022962970.1 (tRNA (cytosine(34)-C(5))-methyltransferase-like [Cucurbita moschata] >KAG6594890.1 RNA cytosine C(5)-methyltransferase NSUN2, partial [Cucurbita argyrosperma subsp. sororia] >KAG7026856.1 tRNA (cytosine(34)-C(5))-methyltransferase [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 1691.0 bits (4378), Expect = 0.0e+00
Identity = 838/838 (100.00%), Postives = 838/838 (100.00%), Query Frame = 0

Query: 1   MGGKGRGGRARTQRKHFRESRENVWKRPKPDPNSANGNKDGNGNHWEPFESQSHAFNEYY 60
           MGGKGRGGRARTQRKHFRESRENVWKRPKPDPNSANGNKDGNGNHWEPFESQSHAFNEYY
Sbjct: 1   MGGKGRGGRARTQRKHFRESRENVWKRPKPDPNSANGNKDGNGNHWEPFESQSHAFNEYY 60

Query: 61  KEQGIVTAEEWDTFIEFLRKPLPAAFRINSSGQFCGEIRAQLQNDFTKSLQAEVTDGDEA 120
           KEQGIVTAEEWDTFIEFLRKPLPAAFRINSSGQFCGEIRAQLQNDFTKSLQAEVTDGDEA
Sbjct: 61  KEQGIVTAEEWDTFIEFLRKPLPAAFRINSSGQFCGEIRAQLQNDFTKSLQAEVTDGDEA 120

Query: 121 EAIRPLPWYPDNLAWHSNFSRMQLRKNQALESFHEFLKLENEIGNITRQEAVSMVPPLFL 180
           EAIRPLPWYPDNLAWHSNFSRMQLRKNQALESFHEFLKLENEIGNITRQEAVSMVPPLFL
Sbjct: 121 EAIRPLPWYPDNLAWHSNFSRMQLRKNQALESFHEFLKLENEIGNITRQEAVSMVPPLFL 180

Query: 181 DVHPNHYVLDMCAAPGSKTFQLLEIIHQSSKPGDLPDGLVVANDLDVQRCNLLIHQTKRM 240
           DVHPNHYVLDMCAAPGSKTFQLLEIIHQSSKPGDLPDGLVVANDLDVQRCNLLIHQTKRM
Sbjct: 181 DVHPNHYVLDMCAAPGSKTFQLLEIIHQSSKPGDLPDGLVVANDLDVQRCNLLIHQTKRM 240

Query: 241 CTANLIVTNHEAQHFPGCRANTNFFSASTAGNELKPRSTQLTFDRVLCDVPCSGDGTLRK 300
           CTANLIVTNHEAQHFPGCRANTNFFSASTAGNELKPRSTQLTFDRVLCDVPCSGDGTLRK
Sbjct: 241 CTANLIVTNHEAQHFPGCRANTNFFSASTAGNELKPRSTQLTFDRVLCDVPCSGDGTLRK 300

Query: 301 APDIWRKWNSGMGNGLHGLQVQIGMRGASLLKVGGRMVYSTCSMNPVENEAVVAELLRKS 360
           APDIWRKWNSGMGNGLHGLQVQIGMRGASLLKVGGRMVYSTCSMNPVENEAVVAELLRKS
Sbjct: 301 APDIWRKWNSGMGNGLHGLQVQIGMRGASLLKVGGRMVYSTCSMNPVENEAVVAELLRKS 360

Query: 361 GGSLELVDVSNELPQLVRRPGLKKWKVCDKGVWFASYEQVTESCQSVAIPGMFPSGRGRK 420
           GGSLELVDVSNELPQLVRRPGLKKWKVCDKGVWFASYEQVTESCQSVAIPGMFPSGRGRK
Sbjct: 361 GGSLELVDVSNELPQLVRRPGLKKWKVCDKGVWFASYEQVTESCQSVAIPGMFPSGRGRK 420

Query: 421 SDTHNSELRGNHPDGGSSVDVSEPTMDPVHVFDEEVCEFPIERCMRIVPHDQNTGAFFVA 480
           SDTHNSELRGNHPDGGSSVDVSEPTMDPVHVFDEEVCEFPIERCMRIVPHDQNTGAFFVA
Sbjct: 421 SDTHNSELRGNHPDGGSSVDVSEPTMDPVHVFDEEVCEFPIERCMRIVPHDQNTGAFFVA 480

Query: 481 VLRKVAPLPAATEKTSDQNTKSANDQSHGKLQQTITDADSVDLNATDGMGESSSEVEIDP 540
           VLRKVAPLPAATEKTSDQNTKSANDQSHGKLQQTITDADSVDLNATDGMGESSSEVEIDP
Sbjct: 481 VLRKVAPLPAATEKTSDQNTKSANDQSHGKLQQTITDADSVDLNATDGMGESSSEVEIDP 540

Query: 541 NNQRDSNSANNNPPCKLPVQDNKDADGLDSTDGMDKQFSEADMEVVENETTENKLEITAI 600
           NNQRDSNSANNNPPCKLPVQDNKDADGLDSTDGMDKQFSEADMEVVENETTENKLEITAI
Sbjct: 541 NNQRDSNSANNNPPCKLPVQDNKDADGLDSTDGMDKQFSEADMEVVENETTENKLEITAI 600

Query: 601 KNNSEESKQPGDGEMDPKKCNGKRKLQIQGKWRGVDPVVLFKDETVIDCIKTFYGINESF 660
           KNNSEESKQPGDGEMDPKKCNGKRKLQIQGKWRGVDPVVLFKDETVIDCIKTFYGINESF
Sbjct: 601 KNNSEESKQPGDGEMDPKKCNGKRKLQIQGKWRGVDPVVLFKDETVIDCIKTFYGINESF 660

Query: 661 PLIGHLVTRNSDTNHVKRIYYISNSVKDVLELNFSAGQQLKITSIGLKMFERQSSREGSS 720
           PLIGHLVTRNSDTNHVKRIYYISNSVKDVLELNFSAGQQLKITSIGLKMFERQSSREGSS
Sbjct: 661 PLIGHLVTRNSDTNHVKRIYYISNSVKDVLELNFSAGQQLKITSIGLKMFERQSSREGSS 720

Query: 721 APCAFRISSEGLPVLLPYITKQILTMSPVDFKHLLQYKSIKYADFVDAAFGEKASNLMLG 780
           APCAFRISSEGLPVLLPYITKQILTMSPVDFKHLLQYKSIKYADFVDAAFGEKASNLMLG
Sbjct: 721 APCAFRISSEGLPVLLPYITKQILTMSPVDFKHLLQYKSIKYADFVDAAFGEKASNLMLG 780

Query: 781 CCVIVLGTGNTSSDVIKVDESTIAIGCWKGRASLSVMVTATDCQELLGRLSNRLGNDL 839
           CCVIVLGTGNTSSDVIKVDESTIAIGCWKGRASLSVMVTATDCQELLGRLSNRLGNDL
Sbjct: 781 CCVIVLGTGNTSSDVIKVDESTIAIGCWKGRASLSVMVTATDCQELLGRLSNRLGNDL 838

BLAST of Carg15981 vs. NCBI nr
Match: XP_023517733.1 (tRNA (cytosine(34)-C(5))-methyltransferase-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 1675.2 bits (4337), Expect = 0.0e+00
Identity = 831/838 (99.16%), Postives = 833/838 (99.40%), Query Frame = 0

Query: 1   MGGKGRGGRARTQRKHFRESRENVWKRPKPDPNSANGNKDGNGNHWEPFESQSHAFNEYY 60
           MGGKGRGGRARTQRKHFRESRENVWKRPKPDPNSAN NKDGNGNHWEPFESQSHAFNEYY
Sbjct: 1   MGGKGRGGRARTQRKHFRESRENVWKRPKPDPNSANDNKDGNGNHWEPFESQSHAFNEYY 60

Query: 61  KEQGIVTAEEWDTFIEFLRKPLPAAFRINSSGQFCGEIRAQLQNDFTKSLQAEVTDGDEA 120
           KEQGIVTAEEW+TFIE LRKPLPAAFRINSSGQFCGEIRAQLQNDFTKSLQAEVTDGDEA
Sbjct: 61  KEQGIVTAEEWNTFIEVLRKPLPAAFRINSSGQFCGEIRAQLQNDFTKSLQAEVTDGDEA 120

Query: 121 EAIRPLPWYPDNLAWHSNFSRMQLRKNQALESFHEFLKLENEIGNITRQEAVSMVPPLFL 180
           EAIRPLPWYPDNLAWHSNFSRMQLRKNQALESFHEFLKLENEIGNITRQEAVSMVPPLFL
Sbjct: 121 EAIRPLPWYPDNLAWHSNFSRMQLRKNQALESFHEFLKLENEIGNITRQEAVSMVPPLFL 180

Query: 181 DVHPNHYVLDMCAAPGSKTFQLLEIIHQSSKPGDLPDGLVVANDLDVQRCNLLIHQTKRM 240
           DVHPNHYVLDMCAAPGSKTFQLLEIIHQSSKPGDLPDGLVVANDLDVQRCNLLIHQTKRM
Sbjct: 181 DVHPNHYVLDMCAAPGSKTFQLLEIIHQSSKPGDLPDGLVVANDLDVQRCNLLIHQTKRM 240

Query: 241 CTANLIVTNHEAQHFPGCRANTNFFSASTAGNELKPRSTQLTFDRVLCDVPCSGDGTLRK 300
           CTANLIVTNHEAQHFPGCRANTNFFSASTAGNELKPRSTQLTFDRVLCDVPCSGDGTLRK
Sbjct: 241 CTANLIVTNHEAQHFPGCRANTNFFSASTAGNELKPRSTQLTFDRVLCDVPCSGDGTLRK 300

Query: 301 APDIWRKWNSGMGNGLHGLQVQIGMRGASLLKVGGRMVYSTCSMNPVENEAVVAELLRKS 360
           APDIWRKWNSGMGNGLHGLQVQIGMRGASLLKVGGRMVYSTCSMNPVENEAVVAELLRKS
Sbjct: 301 APDIWRKWNSGMGNGLHGLQVQIGMRGASLLKVGGRMVYSTCSMNPVENEAVVAELLRKS 360

Query: 361 GGSLELVDVSNELPQLVRRPGLKKWKVCDKGVWFASYEQVTESCQSVAIPGMFPSGRGRK 420
           GGSLELVDVSNELPQLVRRPGLKKWKVCDKGVWFASYEQVTESCQSVAIPGMFPSGRGRK
Sbjct: 361 GGSLELVDVSNELPQLVRRPGLKKWKVCDKGVWFASYEQVTESCQSVAIPGMFPSGRGRK 420

Query: 421 SDTHNSELRGNHPDGGSSVDVSEPTMDPVHVFDEEVCEFPIERCMRIVPHDQNTGAFFVA 480
           SDTHNSELRGNHPDGGSSVDVSEPTMDPVHVFDEEVCEFPIERCMRIVPHDQNTGAFFVA
Sbjct: 421 SDTHNSELRGNHPDGGSSVDVSEPTMDPVHVFDEEVCEFPIERCMRIVPHDQNTGAFFVA 480

Query: 481 VLRKVAPLPAATEKTSDQNTKSANDQSHGKLQQTITDADSVDLNATDGMGESSSEVEIDP 540
           VLRKVAPLPAATEKTSDQNTKSANDQS GKLQQT TDADSVDLNATDGMGESSSEVEIDP
Sbjct: 481 VLRKVAPLPAATEKTSDQNTKSANDQSRGKLQQTTTDADSVDLNATDGMGESSSEVEIDP 540

Query: 541 NNQRDSNSANNNPPCKLPVQDNKDADGLDSTDGMDKQFSEADMEVVENETTENKLEITAI 600
           NNQRDSNSANNNPPCKLPVQDNKDADGLDSTDGMDKQFSEADMEVVENETTENKLEITAI
Sbjct: 541 NNQRDSNSANNNPPCKLPVQDNKDADGLDSTDGMDKQFSEADMEVVENETTENKLEITAI 600

Query: 601 KNNSEESKQPGDGEMDPKKCNGKRKLQIQGKWRGVDPVVLFKDETVIDCIKTFYGINESF 660
           KNNSEESKQPGDGEMDPKKCNGKRKLQIQGKWRGVDPVVLFKDETVIDCIKTFYGINESF
Sbjct: 601 KNNSEESKQPGDGEMDPKKCNGKRKLQIQGKWRGVDPVVLFKDETVIDCIKTFYGINESF 660

Query: 661 PLIGHLVTRNSDTNHVKRIYYISNSVKDVLELNFSAGQQLKITSIGLKMFERQSSREGSS 720
           PLIGHLVTRNSDTNHVKRIYYISNSVKDVLELNFSAGQQLKITSIGLKMFERQSSREGSS
Sbjct: 661 PLIGHLVTRNSDTNHVKRIYYISNSVKDVLELNFSAGQQLKITSIGLKMFERQSSREGSS 720

Query: 721 APCAFRISSEGLPVLLPYITKQILTMSPVDFKHLLQYKSIKYADFVDAAFGEKASNLMLG 780
           APCAFRISSEGLPVLLPYITKQILTMSPVDFKHLLQYKSIKYADFVDAAFGEKASNLMLG
Sbjct: 721 APCAFRISSEGLPVLLPYITKQILTMSPVDFKHLLQYKSIKYADFVDAAFGEKASNLMLG 780

Query: 781 CCVIVLGTGNTSSDVIKVDESTIAIGCWKGRASLSVMVTATDCQELLGRLSNRLGNDL 839
           CCVIVLG GNTSS+VIKVDESTIAIGCWKGRASLSVMVTATDCQELLGRLSNRLGNDL
Sbjct: 781 CCVIVLGAGNTSSEVIKVDESTIAIGCWKGRASLSVMVTATDCQELLGRLSNRLGNDL 838

BLAST of Carg15981 vs. NCBI nr
Match: XP_023003087.1 (tRNA (cytosine(34)-C(5))-methyltransferase-like [Cucurbita maxima])

HSP 1 Score: 1664.4 bits (4309), Expect = 0.0e+00
Identity = 826/838 (98.57%), Postives = 831/838 (99.16%), Query Frame = 0

Query: 1   MGGKGRGGRARTQRKHFRESRENVWKRPKPDPNSANGNKDGNGNHWEPFESQSHAFNEYY 60
           MGGKGRGGRARTQRKHFRESRENVWKRPKPDPNSAN NKDGNGNHWEPFESQS AFNEYY
Sbjct: 1   MGGKGRGGRARTQRKHFRESRENVWKRPKPDPNSANDNKDGNGNHWEPFESQSPAFNEYY 60

Query: 61  KEQGIVTAEEWDTFIEFLRKPLPAAFRINSSGQFCGEIRAQLQNDFTKSLQAEVTDGDEA 120
           KEQGIVTAEEWDTFIE LRKPLPAAFRINSSGQFCGEIRAQLQNDF++SLQAEVTDGDEA
Sbjct: 61  KEQGIVTAEEWDTFIEVLRKPLPAAFRINSSGQFCGEIRAQLQNDFSRSLQAEVTDGDEA 120

Query: 121 EAIRPLPWYPDNLAWHSNFSRMQLRKNQALESFHEFLKLENEIGNITRQEAVSMVPPLFL 180
           EAIRPLPWYPDNLAWHSNFSRMQLRKNQALESFHEFLKLENEIGNITRQEAVSMVPPLFL
Sbjct: 121 EAIRPLPWYPDNLAWHSNFSRMQLRKNQALESFHEFLKLENEIGNITRQEAVSMVPPLFL 180

Query: 181 DVHPNHYVLDMCAAPGSKTFQLLEIIHQSSKPGDLPDGLVVANDLDVQRCNLLIHQTKRM 240
           DVHPNHYVLDMCAAPGSKTFQLLEIIHQSSKPGDLPDGLVVANDLDVQRCNLLIHQTKRM
Sbjct: 181 DVHPNHYVLDMCAAPGSKTFQLLEIIHQSSKPGDLPDGLVVANDLDVQRCNLLIHQTKRM 240

Query: 241 CTANLIVTNHEAQHFPGCRANTNFFSASTAGNELKPRSTQLTFDRVLCDVPCSGDGTLRK 300
           CTANLIVTNHEAQHFPGCRANTNFFSASTAGNELKPRSTQLTFDRVLCDVPCSGDGTLRK
Sbjct: 241 CTANLIVTNHEAQHFPGCRANTNFFSASTAGNELKPRSTQLTFDRVLCDVPCSGDGTLRK 300

Query: 301 APDIWRKWNSGMGNGLHGLQVQIGMRGASLLKVGGRMVYSTCSMNPVENEAVVAELLRKS 360
           APDIWRKWNSGMGNGLHGLQVQIGMRGASLLKVGGRMVYSTCSMNPVENEAVVAELLRKS
Sbjct: 301 APDIWRKWNSGMGNGLHGLQVQIGMRGASLLKVGGRMVYSTCSMNPVENEAVVAELLRKS 360

Query: 361 GGSLELVDVSNELPQLVRRPGLKKWKVCDKGVWFASYEQVTESCQSVAIPGMFPSGRGRK 420
           GGSLELVDVSNELPQLVRRPGLKKWKVCDKGVWFASYEQVTESCQSVAIPGMFPSGRG K
Sbjct: 361 GGSLELVDVSNELPQLVRRPGLKKWKVCDKGVWFASYEQVTESCQSVAIPGMFPSGRGHK 420

Query: 421 SDTHNSELRGNHPDGGSSVDVSEPTMDPVHVFDEEVCEFPIERCMRIVPHDQNTGAFFVA 480
           SDTHNS+LRGNHPDGGSSVDVSEPTMDPVHVFDEEVCEFPIERCMRIVPHDQNTGAFFVA
Sbjct: 421 SDTHNSQLRGNHPDGGSSVDVSEPTMDPVHVFDEEVCEFPIERCMRIVPHDQNTGAFFVA 480

Query: 481 VLRKVAPLPAATEKTSDQNTKSANDQSHGKLQQTITDADSVDLNATDGMGESSSEVEIDP 540
           VLRKVAPL AATEKTSDQNTKSANDQS GKLQQTITDADSVDLNATDGMGESSSEVEIDP
Sbjct: 481 VLRKVAPLLAATEKTSDQNTKSANDQSRGKLQQTITDADSVDLNATDGMGESSSEVEIDP 540

Query: 541 NNQRDSNSANNNPPCKLPVQDNKDADGLDSTDGMDKQFSEADMEVVENETTENKLEITAI 600
           NNQRDSNSANNNPPCKLPVQDNKDADGLDSTDGMDK FSEAD+EVV+NETTENKLEITAI
Sbjct: 541 NNQRDSNSANNNPPCKLPVQDNKDADGLDSTDGMDKPFSEADLEVVDNETTENKLEITAI 600

Query: 601 KNNSEESKQPGDGEMDPKKCNGKRKLQIQGKWRGVDPVVLFKDETVIDCIKTFYGINESF 660
           KNNSEESKQPGDGEMDPKKCNGKRKLQIQGKWRGVDPVVLFKDETVIDCIKTFYGINESF
Sbjct: 601 KNNSEESKQPGDGEMDPKKCNGKRKLQIQGKWRGVDPVVLFKDETVIDCIKTFYGINESF 660

Query: 661 PLIGHLVTRNSDTNHVKRIYYISNSVKDVLELNFSAGQQLKITSIGLKMFERQSSREGSS 720
           PLIGHLVTRNSDTNHVKRIYYISNSVKDVLELNFSAGQQLKITSIGLKMFERQSSREGSS
Sbjct: 661 PLIGHLVTRNSDTNHVKRIYYISNSVKDVLELNFSAGQQLKITSIGLKMFERQSSREGSS 720

Query: 721 APCAFRISSEGLPVLLPYITKQILTMSPVDFKHLLQYKSIKYADFVDAAFGEKASNLMLG 780
           APCAFRISSEGLPVLLPYITKQILTMSPVDFKHLLQYKSIKYADFVDAAFGEKASNLMLG
Sbjct: 721 APCAFRISSEGLPVLLPYITKQILTMSPVDFKHLLQYKSIKYADFVDAAFGEKASNLMLG 780

Query: 781 CCVIVLGTGNTSSDVIKVDESTIAIGCWKGRASLSVMVTATDCQELLGRLSNRLGNDL 839
           CCVIVLGTGNTSSDVIKVDESTIAIGCWKGRASLSVMVTATDCQELLGRLSNRLGNDL
Sbjct: 781 CCVIVLGTGNTSSDVIKVDESTIAIGCWKGRASLSVMVTATDCQELLGRLSNRLGNDL 838

BLAST of Carg15981 vs. NCBI nr
Match: XP_038882187.1 (RNA cytosine C(5)-methyltransferase NSUN2-like [Benincasa hispida])

HSP 1 Score: 1432.5 bits (3707), Expect = 0.0e+00
Identity = 735/853 (86.17%), Postives = 777/853 (91.09%), Query Frame = 0

Query: 1   MGGKGRGGRARTQRKHFRESRENVWKRPKPDPNSANGNKD----GNGNHWEPFESQSHAF 60
           MGGKGRGGRARTQRKHFR++RENVWKRP+ DPNSAN NKD    GNGN WEPFESQS AF
Sbjct: 1   MGGKGRGGRARTQRKHFRDNRENVWKRPRSDPNSANDNKDGNGNGNGNPWEPFESQSPAF 60

Query: 61  NEYYKEQGIVTAEEWDTFIEFLRKPLPAAFRINSSGQFCGEIRAQLQNDFTKSLQAEVTD 120
           NEYYKEQGIV+ EEW+TFIE LRKPLPAAFRINSSGQFC EIRAQLQNDF KSLQAEVT+
Sbjct: 61  NEYYKEQGIVSEEEWNTFIEVLRKPLPAAFRINSSGQFCDEIRAQLQNDFMKSLQAEVTE 120

Query: 121 GDEAEAIRPLPWYPDNLAWHSNFSRMQLRKNQALESFHEFLKLENEIGNITRQEAVSMVP 180
           GDEAEAIRPLPWYPDNLAWHSNFSRMQLRKNQALE FHEFLKLENEIGNITRQEAVSMVP
Sbjct: 121 GDEAEAIRPLPWYPDNLAWHSNFSRMQLRKNQALERFHEFLKLENEIGNITRQEAVSMVP 180

Query: 181 PLFLDVHPNHYVLDMCAAPGSKTFQLLEIIHQSSKPGDLPDGLVVANDLDVQRCNLLIHQ 240
           PLFLDVHPNHYVLDMCAAPGSKTFQLLEIIH+SSKPGDLPDGLVVANDLDVQRCNLLIHQ
Sbjct: 181 PLFLDVHPNHYVLDMCAAPGSKTFQLLEIIHKSSKPGDLPDGLVVANDLDVQRCNLLIHQ 240

Query: 241 TKRMCTANLIVTNHEAQHFPGCRANTNFFSASTAGNELKPRSTQLTFDRVLCDVPCSGDG 300
           TKRMCTANLIVTNHEAQHFPGCRA+T+FF+AS AG+E+KP +TQLTFDRVLCDVPCSGDG
Sbjct: 241 TKRMCTANLIVTNHEAQHFPGCRAHTSFFNASAAGSEVKPHNTQLTFDRVLCDVPCSGDG 300

Query: 301 TLRKAPDIWRKWNSGMGNGLHGLQVQIGMRGASLLKVGGRMVYSTCSMNPVENEAVVAEL 360
           TLRKAPDIWRKWNSGMGNGLHGLQVQIGMRGASLLKVGGRMVYSTCSMNPVENEAVVAEL
Sbjct: 301 TLRKAPDIWRKWNSGMGNGLHGLQVQIGMRGASLLKVGGRMVYSTCSMNPVENEAVVAEL 360

Query: 361 LRKSGGSLELVDVSNELPQLVRRPGLKKWKVCDKGVWFASYEQVTESCQSVAIPGMFPSG 420
           LRKSGGSLELVDVSNELPQLV RPGLKKWKV D+GVW ASY+++ E  QSVAIP MFPSG
Sbjct: 361 LRKSGGSLELVDVSNELPQLVCRPGLKKWKVRDRGVWLASYKEIIEGRQSVAIPSMFPSG 420

Query: 421 RGRKS---DTHNSELRGNHPDG--GSSVDVSEPTMDPVHVFDEEVCEFPIERCMRIVPHD 480
           RGRK+   + HNSEL GNH DG  GSSVDVSEP MDPV  FD EVCEFPIERC+RIVPHD
Sbjct: 421 RGRKNQADNNHNSELGGNHLDGSIGSSVDVSEPIMDPVLEFD-EVCEFPIERCIRIVPHD 480

Query: 481 QNTGAFFVAVLRKVAPLPAATEKTSDQNTKSANDQSHGKLQQTITDADSVDLNATDGMGE 540
           QN+GAFF+AVLRKVAPLPAA EK   QN+KSA+D+S  KL QTI D +SV+++A  GM E
Sbjct: 481 QNSGAFFIAVLRKVAPLPAAVEK---QNSKSADDRSRCKLPQTIADGESVNVDAAVGMDE 540

Query: 541 SSSEVEIDPNNQRDSNSANNNPPCKLPVQDNKDADGLD--STDGMDKQFSEADME---VV 600
           S SEV IDPNNQ D NSA+N P C+LPVQD KDADGLD  + DG  KQFSEAD+    V 
Sbjct: 541 SPSEVGIDPNNQEDFNSADNKPACELPVQDIKDADGLDTNAVDGRGKQFSEADLAAKLVD 600

Query: 601 ENETTENKLEITAIKNNSEESKQPGDGEMDPKKCNGKRKLQIQGKWRGVDPVVLFKDETV 660
            NE  EN LE+TAIK+NSEESKQP DGEMDPKK + KRKLQIQGKW+GVDPVVLFKDETV
Sbjct: 601 NNEVDENNLEVTAIKDNSEESKQPDDGEMDPKKSSVKRKLQIQGKWKGVDPVVLFKDETV 660

Query: 661 IDCIKTFYGINESFPLIGHLVTRNSDTNHVKRIYYISNSVKDVLELNFSAGQQLKITSIG 720
           ID IKTFYGINESFPLIGHLVTRNSDTNHVKRIYYISNSVKDVLELNF+ GQQLKITSIG
Sbjct: 661 IDSIKTFYGINESFPLIGHLVTRNSDTNHVKRIYYISNSVKDVLELNFAVGQQLKITSIG 720

Query: 721 LKMFERQSSREGSSAPCAFRISSEGLPVLLPYITKQILTMSPVDFKHLLQYKSIKYADFV 780
           LKMFERQSSREGSSAPC+FRISSEGLPV+LP+ITKQILTMSPVDFKHLLQYKSIKYADFV
Sbjct: 721 LKMFERQSSREGSSAPCSFRISSEGLPVILPHITKQILTMSPVDFKHLLQYKSIKYADFV 780

Query: 781 DAAFGEKASNLMLGCCVIVLGTG-NTSSDVIKVDESTIAIGCWKGRASLSVMVTATDCQE 839
           DAAFGEKASNLMLGCCVIVLGTG NTSSDVIKVDESTIAIGCWKGRASLSVMVTA DCQE
Sbjct: 781 DAAFGEKASNLMLGCCVIVLGTGSNTSSDVIKVDESTIAIGCWKGRASLSVMVTAIDCQE 840

BLAST of Carg15981 vs. NCBI nr
Match: KAG6604044.1 (RNA cytosine C(5)-methyltransferase NSUN2, partial [Cucurbita argyrosperma subsp. sororia] >KAG7034210.1 tRNA (cytosine(34)-C(5))-methyltransferase, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 1421.4 bits (3678), Expect = 0.0e+00
Identity = 724/848 (85.38%), Postives = 768/848 (90.57%), Query Frame = 0

Query: 1   MGGKGRGGRARTQRKHFRESRENVWKRPKPDPNSANGNKDGNGNHWEPFESQSHAFNEYY 60
           MGGKGRGGRARTQR HFRESRENVWKR KPD NSAN  KDGN NHW PF+SQSHAFNEYY
Sbjct: 1   MGGKGRGGRARTQRNHFRESRENVWKRSKPDSNSANDTKDGNSNHWGPFQSQSHAFNEYY 60

Query: 61  KEQGIVTAEEWDTFIEFLRKPLPAAFRINSSGQFCGEIRAQLQNDFTKSLQAEVTDGDEA 120
           KEQGIVTAEEWDTFIE LRK LPAAFRINSSGQFCGEIRAQLQNDF KSLQ+EVTDGDEA
Sbjct: 61  KEQGIVTAEEWDTFIEVLRKQLPAAFRINSSGQFCGEIRAQLQNDFMKSLQSEVTDGDEA 120

Query: 121 EAIRPLPWYPDNLAWHSNFSRMQLRKNQALESFHEFLKLENEIGNITRQEAVSMVPPLFL 180
            AIRPL WYPDNLAWHSNFSRMQLRKNQALE FHEFLKLENEIGNITRQEAVSMVPPLFL
Sbjct: 121 GAIRPLSWYPDNLAWHSNFSRMQLRKNQALERFHEFLKLENEIGNITRQEAVSMVPPLFL 180

Query: 181 DVHPNHYVLDMCAAPGSKTFQLLEIIHQSSKPGDLPDGLVVANDLDVQRCNLLIHQTKRM 240
           DVH NHYVLDMCAAPGSKTFQLLEIIHQSSKPGDLP GLVVAND DVQRCNLLIHQTKRM
Sbjct: 181 DVHRNHYVLDMCAAPGSKTFQLLEIIHQSSKPGDLPGGLVVANDSDVQRCNLLIHQTKRM 240

Query: 241 CTANLIVTNHEAQHFPGCRANTNFFSASTAGNELKPRSTQLTFDRVLCDVPCSGDGTLRK 300
           CT+NLIVTNHEAQHFPGCRA+TNFF+AS AG EL   +TQLTFDRVLCDVPCSGDGTLRK
Sbjct: 241 CTSNLIVTNHEAQHFPGCRAHTNFFNASAAGIELNHHNTQLTFDRVLCDVPCSGDGTLRK 300

Query: 301 APDIWRKWNSGMGNGLHGLQVQIGMRGASLLKVGGRMVYSTCSMNPVENEAVVAELLRKS 360
           APDIWR+WNSG+GNGLHGLQVQIGMRGASLLKVGGRMVYSTCSMNPVENEAVVAELLRKS
Sbjct: 301 APDIWRRWNSGVGNGLHGLQVQIGMRGASLLKVGGRMVYSTCSMNPVENEAVVAELLRKS 360

Query: 361 GGSLELVDVSNELPQLVRRPGLKKWKVCDKGVWFASYEQVTESCQSVAIPGMFPSGRGRK 420
            GSLELVDVSNELPQLVRRPGLKKWKV D+GVW ASY+QV++  QSVAIP MFPSGRGRK
Sbjct: 361 EGSLELVDVSNELPQLVRRPGLKKWKVRDRGVWLASYKQVSDHRQSVAIPSMFPSGRGRK 420

Query: 421 SD---THNSELRGNHPDG--GSSVDVSEPTMDPVHVFDEEVCEFPIERCMRIVPHDQNTG 480
           +    +HNSELRGNH DG  GSSVDVSEPTMDPVH FD EVCEFPIERCMRIVPHDQN+G
Sbjct: 421 NQADKSHNSELRGNHLDGSIGSSVDVSEPTMDPVHEFD-EVCEFPIERCMRIVPHDQNSG 480

Query: 481 AFFVAVLRKVAPLPAATEKTSDQNTKSANDQSHGKLQQTITDADSVDLNATDGMGESSSE 540
           AFF+AVLRKVAPLPAATEK S QN K  ND+S  KL QTIT+  +VD++A+ GM ES SE
Sbjct: 481 AFFIAVLRKVAPLPAATEKPSYQNLKPVNDKSRCKLPQTITNGANVDVSASVGMDESPSE 540

Query: 541 VEIDPNNQRDSNSANNNPPCKLPVQDNKDADGLDS--TDGMDKQFSEADMEV--VENETT 600
           V ID NNQ D NSA+N P C+ PVQD +  +GLDS   DG+DKQFSEAD+EV  V+NE +
Sbjct: 541 VGIDSNNQGDFNSADNKPACEFPVQDIQGTEGLDSNAADGIDKQFSEADLEVNLVDNEVS 600

Query: 601 ENKLEITAIKNNSEESKQPGDGEMDPKKCNGKRKLQIQGKWRGVDPVVLFKDETVIDCIK 660
            N LEITAI++N EESKQP DGEMDPKK   KRKLQIQGKW+GVDPVVLFKDETVID IK
Sbjct: 601 VNNLEITAIEDNLEESKQPDDGEMDPKKSTVKRKLQIQGKWKGVDPVVLFKDETVIDSIK 660

Query: 661 TFYGINESFPLIGHLVTRNSDTNHVKRIYYISNSVKDVLELNFSAGQQLKITSIGLKMFE 720
           TFYGINE+FPL GHLVTRN+DTNHVKRIYY+S SVKDVLELNF+ GQQLKITSIGLKMFE
Sbjct: 661 TFYGINEAFPLFGHLVTRNTDTNHVKRIYYVSKSVKDVLELNFAVGQQLKITSIGLKMFE 720

Query: 721 RQSSREGSSAPCAFRISSEGLPVLLPYITKQILTMSPVDFKHLLQYKSIKYADFVDAAFG 780
           RQSSREGSSAPC+FRI+SEGLPV+LPYITKQILTMSPVDFKHL+Q+KSIKYADF DAAFG
Sbjct: 721 RQSSREGSSAPCSFRITSEGLPVILPYITKQILTMSPVDFKHLIQHKSIKYADFADAAFG 780

Query: 781 EKASNLMLGCCVIVLGTG-NTSSDVIKVDESTIAIGCWKGRASLSVMVTATDCQELLGRL 839
           EKASNLMLGCCVIVLGTG NTSSDVIKVDESTIAIGCWKGR+SLSVMVTA DCQELL RL
Sbjct: 781 EKASNLMLGCCVIVLGTGSNTSSDVIKVDESTIAIGCWKGRSSLSVMVTAIDCQELLERL 840

BLAST of Carg15981 vs. ExPASy Swiss-Prot
Match: Q1HFZ0 (RNA cytosine C(5)-methyltransferase NSUN2 OS=Mus musculus OX=10090 GN=Nsun2 PE=1 SV=2)

HSP 1 Score: 426.0 bits (1094), Expect = 9.7e-118
Identity = 285/799 (35.67%), Postives = 414/799 (51.81%), Query Frame = 0

Query: 27  RPKPDPNSANGNKDGNGNHWE----PFESQSHAFNEYYKEQGIVTAEEWDTFIEFLRKPL 86
           +P+ + ++++G +      WE        ++  F  YY+E  IV   EWD F+E LR+PL
Sbjct: 15  QPEGEEDASDGGRKRGQAGWEGGYPEIVKENKLFEHYYQELKIVPEGEWDQFMESLREPL 74

Query: 87  PAAFRINSSGQFCGEIRAQLQNDFTKSLQAEVTDGDEAEAIRPLPWYPDNLAWHSNFSRM 146
           PA  RI        EI   L+N + K L+    DG + E  +PL WYP+ LAWH+N SR 
Sbjct: 75  PATLRITGYKSHAKEILHCLKNKYFKELEDLEVDGQKVEVPQPLSWYPEELAWHTNLSRK 134

Query: 147 QLRKNQALESFHEFLKLENEIGNITRQEAVSMVPPLFLDVHPNHYVLDMCAAPGSKTFQL 206
            LRK+  L  FH+FL  E E GNI+RQEAVSM+PPL L+V P+H +LDMCAAPGSKT QL
Sbjct: 135 ILRKSPLLAKFHQFLVSETESGNISRQEAVSMIPPLLLNVEPHHKILDMCAAPGSKTTQL 194

Query: 207 LEIIHQSSKPGDLPDGLVVANDLDVQRCNLLIHQTKRMCTANLIVTNHEAQHFPGCRANT 266
           +E++H +      P+G V+AND+D +RC LL+HQ KR+ +  ++V NH+A   P      
Sbjct: 195 IEMLH-ADMSVPFPEGFVIANDVDNKRCYLLVHQAKRLSSPCIMVVNHDASSIPRLTV-- 254

Query: 267 NFFSASTAGNELKPRSTQLTFDRVLCDVPCSGDGTLRKAPDIWRKWNSGMGNGLHGLQVQ 326
                     ++  R   L +DR+LCDVPCSGDGT+RK  D+W+KW +     LHGLQ++
Sbjct: 255 ----------DVDGRKEILFYDRILCDVPCSGDGTMRKNIDVWKKWTTLNSLQLHGLQLR 314

Query: 327 IGMRGASLLKVGGRMVYSTCSMNPVENEAVVAELLRKSGGSLELVDVSNELPQLVRRPGL 386
           I  RGA  L  GGRMVYSTCS+NPVE+EAV+A LL KS G+LEL DVS ELP L   PG+
Sbjct: 315 IATRGAEQLAEGGRMVYSTCSLNPVEDEAVIAALLEKSEGALELADVSAELPGLKWMPGV 374

Query: 387 KKWKVCDK-GVWFASYEQVTESCQSVAIPGMFPSGRGRKSDTHNSELRGNHPDGGSSVDV 446
            +WKV  + G WFA + +V +   +   P MFP        T   +L+  H         
Sbjct: 375 SQWKVMTRDGQWFADWHEVPQGRHTQIRPTMFP-------PTDLEKLQAMH--------- 434

Query: 447 SEPTMDPVHVFDEEVCEFPIERCMRIVPHDQNTGAFFVAVLRKVAPLPAATEKTSDQNTK 506
                              +ERC+RI+PH QNTG FFVAVL K AP+P    +   QN K
Sbjct: 435 -------------------LERCLRILPHHQNTGGFFVAVLVKKAPMPWNKRQPKVQN-K 494

Query: 507 SANDQSHGKLQQTITDADSVDLNATDGMGESSSEVEIDP-NNQRDSNSANNNPPCKLPVQ 566
           SA  +             S  + AT+G     SE+E        DS +A+N         
Sbjct: 495 SAEARE---------PRVSSHVAATEGNPSDQSELESQMITGAGDSETAHN--------- 554

Query: 567 DNKDADGLDSTDGMDKQFSEADMEVVENETTENKLEITAIKNNSEESKQPGDGEMDPKKC 626
                                         TEN            ES +  DG   P   
Sbjct: 555 ------------------------------TEN-----------TESNEKKDGVCGPPP- 614

Query: 627 NGKRKLQIQGKWRGVDPVVLF-KDETVIDCIKTFYGINESFPLIGHLVTRNSDTNHVKRI 686
              +K+++ G     DP V   +D+ +   I+ FY ++ SFP + +L+TR ++    +++
Sbjct: 615 --SKKMKLFGFKE--DPFVFIPEDDPLFPPIEKFYALDPSFPRM-NLLTRTTE-GKKRQL 674

Query: 687 YYISNSVKDVLELNFSAGQQLKITSIGLKMFERQSSREGSSAPCAFRISSEGLPVLLPYI 746
           Y +S  +++VL  N    +++K+ + G+K++ R +S  G    CAFR++ EG+  L P+I
Sbjct: 675 YMVSKELRNVLLNN---SEKMKVINTGIKVWCRNNS--GEEFDCAFRLAQEGIYTLYPFI 689

Query: 747 TKQILTMSPVDFKHLLQYKSIKYADFVDAAFGEKASNLMLGCCVIVLGTGNTSSDVIKVD 806
             +I+T+S  D K LL  ++  +      A+ +   +L  G  V+     + + D +   
Sbjct: 735 NSRIITVSMEDVKTLLTQENPFFRKLSSEAYSQ-VKDLAKGSVVLKYEPDSANPDTL--- 689

Query: 807 ESTIAIGCWKGRASLSVMV 819
           +  I +  W+G+AS+   V
Sbjct: 795 QCPIVLCGWRGKASIRTFV 689

BLAST of Carg15981 vs. ExPASy Swiss-Prot
Match: Q5ZLV4 (RNA cytosine-C(5)-methyltransferase NSUN2 OS=Gallus gallus OX=9031 GN=NSUN2 PE=2 SV=1)

HSP 1 Score: 417.5 bits (1072), Expect = 3.5e-115
Identity = 281/815 (34.48%), Postives = 410/815 (50.31%), Query Frame = 0

Query: 7   GGRARTQRKHFRESRENVWKRPKPDPNSANG-NKDGNGNHWEPFESQSHAFNEYYKEQGI 66
           G RAR +R+  +  +    +R +       G ++ G    +     ++  F  YY+EQ I
Sbjct: 2   GRRARDRRRQLQPQQ----RRERSGGGGGGGDDQAGWAGGYPEIVKENELFERYYREQRI 61

Query: 67  VTAEEWDTFIEFLRKPLPAAFRINSSGQFCGEIRAQLQNDFTKSLQAEVTDGDEAEAIRP 126
           V   EWD F+  LR+PLPA  RI        EI   L+  + + LQ    DG + E  + 
Sbjct: 62  VPDGEWDAFMAALREPLPATLRITGYKSHAREILHCLKEKYFRELQHLEVDGQKVEMPQA 121

Query: 127 LPWYPDNLAWHSNFSRMQLRKNQALESFHEFLKLENEIGNITRQEAVSMVPPLFLDVHPN 186
           L WYP+ LAWH+N SR  LRK+  LE FH+FL  E E GNI+RQEAVSM+PPL L+V+P+
Sbjct: 122 LSWYPEELAWHTNLSRKILRKSPQLERFHQFLVSETECGNISRQEAVSMIPPLLLNVNPD 181

Query: 187 HYVLDMCAAPGSKTFQLLEIIHQSSKPGDLPDGLVVANDLDVQRCNLLIHQTKRMCTANL 246
           H +LDMCAAPGSKT QL+E++H        P G V+AND+D +RC LL+HQ KR+ +  +
Sbjct: 182 HKILDMCAAPGSKTAQLIEMLHADMNV-PFPKGFVIANDVDNKRCYLLVHQAKRLNSPCI 241

Query: 247 IVTNHEAQHFPGCRANTNFFSASTAGNELKPRSTQLTFDRVLCDVPCSGDGTLRKAPDIW 306
           +V NH+A   P  +             ++  R   L +DR+LCDVPCSGDGT+RK  D+W
Sbjct: 242 MVVNHDASSIPNLQV------------DVDGRKETLFYDRILCDVPCSGDGTMRKNIDVW 301

Query: 307 RKWNSGMGNGLHGLQVQIGMRGASLLKVGGRMVYSTCSMNPVENEAVVAELLRKSGGSLE 366
           +KW +     LHGLQ++I  RG   L  GGRMVYSTCS+NP+ENEAV+A LL KS G+LE
Sbjct: 302 KKWTTQNSLQLHGLQLRIATRGVEQLAEGGRMVYSTCSLNPIENEAVIASLLEKSQGALE 361

Query: 367 LVDVSNELPQLVRRPGLKKWKVCDK-GVWFASYEQVTESCQSVAIPGMFPSGRGRKSDTH 426
           L DVS+ELP L R PG+ KWKV  K G WF  ++ V  + Q+   P MFP          
Sbjct: 362 LADVSSELPGLKRMPGITKWKVMLKDGQWFEEWKDVPSNRQTQIRPTMFP---------- 421

Query: 427 NSELRGNHPDGGSSVDVSEPTMDPVHVFDEEVCEFPIERCMRIVPHDQNTGAFFVAVLRK 486
                           + E         +E++    +ERC+RI+PH QNTG FFVAVL K
Sbjct: 422 ----------------IKE---------EEKLKAMNLERCIRILPHHQNTGGFFVAVLIK 481

Query: 487 VAPLPAATEKTSDQNTKSANDQSHGKLQQTITDADSVDLNATDGMGESSSEVEIDPNNQR 546
            +P+P                + H KL     D +    NA DG                
Sbjct: 482 KSPMP----------WNKRQPKVHQKLPGKTEDTEVTATNAGDG---------------- 541

Query: 547 DSNSANNNPPCKLPVQDNKDADGLDSTDGMDKQFSEADMEVVENETTENKLEITAIKNNS 606
                                    S D  +K     D         E   ++  ++N+ 
Sbjct: 542 -------------------------SEDATEKPTLAED---------EEPKKVQELQNSD 601

Query: 607 EESKQPGDGEMDPKKCNGKRKLQIQGKWRGVDP-VVLFKDETVIDCIKTFYGINESFPLI 666
            E  + G     P K     K+++ G     DP V L +D+ +   I+ FY ++ SFP +
Sbjct: 602 TEQSKKGVCGPPPSK-----KMKLFGFKE--DPFVFLPEDDPLFLPIQKFYALDPSFPKM 661

Query: 667 GHLVTRNSDTNHVKRIYYISNSVKDVLELNFSAGQQLKITSIGLKMFERQSSREGSSAPC 726
            +L+TR  +    +++Y +S  +++VL  N    +++K+ + G+K++ R S  +G    C
Sbjct: 662 -NLLTRTQE-GKKRQLYMVSKELRNVLLNN---SEKMKVINTGIKVWSRNS--DGEQFGC 686

Query: 727 AFRISSEGLPVLLPYITKQILTMSPVDFKHLLQYKSIKYADFVDAAFGEKASNLMLGCCV 786
           AFR++ EG+  L P+I  +I+ +   D K LL  ++   + F  +    K  ++ +G  V
Sbjct: 722 AFRLAQEGIYTLYPFIHARIVNVCIEDVKILLTQENPFLSKF-SSETQRKVKDMAMGSIV 686

Query: 787 IVLGTGNTSSDVIKVDESTIAIGCWKGRASLSVMV 819
           +         D +   +  I +  W+G+ SL   V
Sbjct: 782 LKYEPDPEKPDDL---QCPIVLCGWQGKTSLRAFV 686

BLAST of Carg15981 vs. ExPASy Swiss-Prot
Match: Q08J23 (RNA cytosine C(5)-methyltransferase NSUN2 OS=Homo sapiens OX=9606 GN=NSUN2 PE=1 SV=2)

HSP 1 Score: 416.8 bits (1070), Expect = 5.9e-115
Identity = 285/824 (34.59%), Postives = 415/824 (50.36%), Query Frame = 0

Query: 1   MGGKGRGGRARTQRKHFRESRENVWKRPKPDPNSANGNKDGNGNHWE----PFESQSHAF 60
           MG + RG R + Q            +RP+   + A G        WE        ++  F
Sbjct: 1   MGRRSRGRRLQQQ------------QRPEDAEDGAEGGGKRGEAGWEGGYPEIVKENKLF 60

Query: 61  NEYYKEQGIVTAEEWDTFIEFLRKPLPAAFRINSSGQFCGEIRAQLQNDFTKSLQAEVTD 120
             YY+E  IV   EW  F++ LR+PLPA  RI        EI   L+N + K L+    D
Sbjct: 61  EHYYQELKIVPEGEWGQFMDALREPLPATLRITGYKSHAKEILHCLKNKYFKELEDLEVD 120

Query: 121 GDEAEAIRPLPWYPDNLAWHSNFSRMQLRKNQALESFHEFLKLENEIGNITRQEAVSMVP 180
           G + E  +PL WYP+ LAWH+N SR  LRK+  LE FH+FL  E E GNI+RQEAVSM+P
Sbjct: 121 GQKVEVPQPLSWYPEELAWHTNLSRKILRKSPHLEKFHQFLVSETESGNISRQEAVSMIP 180

Query: 181 PLFLDVHPNHYVLDMCAAPGSKTFQLLEIIHQSSKPGDLPDGLVVANDLDVQRCNLLIHQ 240
           PL L+V P+H +LDMCAAPGSKT QL+E++H        P+G V+AND+D +RC LL+HQ
Sbjct: 181 PLLLNVRPHHKILDMCAAPGSKTTQLIEMLHADMNV-PFPEGFVIANDVDNKRCYLLVHQ 240

Query: 241 TKRMCTANLIVTNHEAQHFPGCRANTNFFSASTAGNELKPRSTQLTFDRVLCDVPCSGDG 300
            KR+ +  ++V NH+A   P  +             ++  R   L +DR+LCDVPCSGDG
Sbjct: 241 AKRLSSPCIMVVNHDASSIPRLQI------------DVDGRKEILFYDRILCDVPCSGDG 300

Query: 301 TLRKAPDIWRKWNSGMGNGLHGLQVQIGMRGASLLKVGGRMVYSTCSMNPVENEAVVAEL 360
           T+RK  D+W+KW +     LHGLQ++I  RGA  L  GGRMVYSTCS+NP+E+EAV+A L
Sbjct: 301 TMRKNIDVWKKWTTLNSLQLHGLQLRIATRGAEQLAEGGRMVYSTCSLNPIEDEAVIASL 360

Query: 361 LRKSGGSLELVDVSNELPQLVRRPGLKKWKVCDK-GVWFASYEQVTESCQSVAIPGMFPS 420
           L KS G+LEL DVSNELP L   PG+ +WKV  K G WF  ++ V  S  +   P MF  
Sbjct: 361 LEKSEGALELADVSNELPGLKWMPGITQWKVMTKDGQWFTDWDAVPHSRHTQIRPTMF-- 420

Query: 421 GRGRKSDTHNSELRGNHPDGGSSVDVSEPTMDPVHVFDEEVCEFPIERCMRIVPHDQNTG 480
                                       P  DP     E++    +ERC+RI+PH QNTG
Sbjct: 421 ----------------------------PPKDP-----EKLQAMHLERCLRILPHHQNTG 480

Query: 481 AFFVAVLRKVAPLPAATEKTSDQNTKSANDQSHGKLQQTITDADSVDLNATDGMGESSSE 540
            FFVAVL K + +P               ++   KLQ                 G+S+  
Sbjct: 481 GFFVAVLVKKSSMPW--------------NKRQPKLQ-----------------GKSAET 540

Query: 541 VEIDPNNQRDSNSANNNPPCKLPVQDNKDADGLDSTDGMDKQFSEADMEVVENETTENKL 600
            E    +  D        P KL      ++     T   +   +  D+E           
Sbjct: 541 RESTQLSPADLTEGKPTDPSKL------ESPSFTGTGDTEIAHATEDLE----------- 600

Query: 601 EITAIKNNSEESKQPGDGEMDPKKCNGKRKLQIQGKWRGVDPVVLF-KDETVIDCIKTFY 660
                 NN  +     DG   P      +K+++ G     DP V   +D+ +   I+ FY
Sbjct: 601 ------NNGSKK----DGVCGPPP---SKKMKLFGFKE--DPFVFIPEDDPLFPPIEKFY 660

Query: 661 GINESFPLIGHLVTRNSDTNHVKRIYYISNSVKDVLELNFSAGQQLKITSIGLKMFERQS 720
            ++ SFP + +L+TR ++    +++Y +S  +++VL  N    +++K+ + G+K++ R +
Sbjct: 661 ALDPSFPRM-NLLTRTTE-GKKRQLYMVSKELRNVLLNN---SEKMKVINTGIKVWCRNN 690

Query: 721 SREGSSAPCAFRISSEGLPVLLPYITKQILTMSPVDFKHLLQYKSIKYADFVDAAFGEKA 780
           S  G    CAFR++ EG+  L P+I  +I+T+S  D K LL  ++  +       + + A
Sbjct: 721 S--GEEFDCAFRLAQEGIYTLYPFINSRIITVSMEDVKILLTQENPFFRKLSSETYSQ-A 690

Query: 781 SNLMLGCCVIVLGTGNTSSDVIKVDESTIAIGCWKGRASLSVMV 819
            +L  G  V+     + + D +   +  I +  W+G+AS+   V
Sbjct: 781 KDLAKGSIVLKYEPDSANPDAL---QCPIVLCGWRGKASIRTFV 690

BLAST of Carg15981 vs. ExPASy Swiss-Prot
Match: Q28E61 (RNA cytosine-C(5)-methyltransferase NSUN2 OS=Xenopus tropicalis OX=8364 GN=nsun2 PE=2 SV=1)

HSP 1 Score: 405.2 bits (1040), Expect = 1.8e-111
Identity = 284/826 (34.38%), Postives = 405/826 (49.03%), Query Frame = 0

Query: 1   MGGKGRGGRARTQRKHFRESRENVWKRPKPDPNSANGNKDGNGNHWE----PFESQSHAF 60
           MG + R  R R QR   + S     +R K    +A          WE        ++  F
Sbjct: 1   MGRRNRRNRQRHQRSTEQRSPAEEEQRRKAREQAA----------WECGYPEIIKENKLF 60

Query: 61  NEYYKEQGIVTAEEWDTFIEFLRKPLPAAFRINSSGQFCGEIRAQLQNDFTKSLQAEVTD 120
             YY+E  IV   EWD F+  LR+PLPA  RI        EI   L+  + K LQ    D
Sbjct: 61  EHYYQELKIVPDGEWDKFMASLREPLPATIRITGYKSHAKEILHCLKEKYFKELQDIEVD 120

Query: 121 GDEAEAIRPLPWYPDNLAWHSNFSRMQLRKNQALESFHEFLKLENEIGNITRQEAVSMVP 180
           G + EA +PL WYP+ LAWH+N SR  +RK+  LE FH+FL  E E GNI+RQEAVSM+P
Sbjct: 121 GQKIEAPQPLSWYPEELAWHTNLSRKIIRKSPELEKFHQFLVNETESGNISRQEAVSMIP 180

Query: 181 PLFLDVHPNHYVLDMCAAPGSKTFQLLEIIHQSSKPGDLPDGLVVANDLDVQRCNLLIHQ 240
           P+ L V P+H +LDMCAAPGSKT Q++E++H        P+G V+AND+D +RC LL+HQ
Sbjct: 181 PVLLKVQPHHKILDMCAAPGSKTAQIIEMLHADMNV-PFPEGFVIANDVDNKRCYLLVHQ 240

Query: 241 TKRMCTANLIVTNHEAQHFPGCRANTNFFSASTAGNELKPRSTQLTFDRVLCDVPCSGDG 300
            KR+ +  ++V NH+A   P      N         E+      L +DR+LCDVPCSGDG
Sbjct: 241 AKRLNSPCIMVVNHDASSIPRLLVENN------GSREV------LYYDRILCDVPCSGDG 300

Query: 301 TLRKAPDIWRKWNSGMGNGLHGLQVQIGMRGASLLKVGGRMVYSTCSMNPVENEAVVAEL 360
           TLRK  D+W+KW +     LHGLQ++I  RG   L  GGRMVYSTCS+NPVE+EAV+A L
Sbjct: 301 TLRKNIDVWKKWTTLNSLQLHGLQIRIATRGVEQLAEGGRMVYSTCSLNPVEDEAVIASL 360

Query: 361 LRKSGGSLELVDVSNELPQLVRRPGLKKWKVCDK-GVWFASYEQVTESCQSVAIPGMFPS 420
           L KS GSLEL DV++E+P L   PG+ +WKV  K G W+  +E +  S  +   P MFP 
Sbjct: 361 LDKSEGSLELADVASEIPGLKWMPGITQWKVMTKEGHWYEKWEDIPTSRHTQIRPTMFPP 420

Query: 421 GRGRKSDTHNSELRGNHPDGGSSVDVSEPTMDPVHVFDEEVCEFPIERCMRIVPHDQNTG 480
               K  + N                                   + RCMRI+PH QNTG
Sbjct: 421 KDEEKLKSMN-----------------------------------LNRCMRILPHHQNTG 480

Query: 481 AFFVAVLRKVAPLP--AATEKTSDQNTKSANDQSHGKLQQTITDADSVDLNATDGMGESS 540
            FFVAVL K AP+P      K   +   SA D S       I  A  +    T+     +
Sbjct: 481 GFFVAVLIKKAPMPWNKRQPKLQRRPPVSACDAS-------IAVAPELVKAVTENSAGMA 540

Query: 541 SEVEIDPNNQRDSNSANNNPPCKLPVQDNKDADGLDSTDGMDKQFSEADMEVVENETTEN 600
            E  +D  N           PC                                N++  +
Sbjct: 541 DEPAVDTEN-------GETKPC-------------------------------TNQSDSS 600

Query: 601 KLEITAIKNNSEESKQPGDGEMDPKKCNGKRKLQIQGKWRGVDP-VVLFKDETVIDCIKT 660
           K +I      S++ K  G  E                     DP V + +D+ + D I+T
Sbjct: 601 KTDIVCCPPPSKKMKLFGFKE---------------------DPFVFVSEDDPIFDPIQT 660

Query: 661 FYGINESFPLIGHLVTRNSDTNHVKRIYYISNSVKDVLELNFSAGQQLKITSIGLKMFER 720
           FY ++ SFP   +L+TR  +    +++Y +S  +++VL  N    +++K+ + G+K+  R
Sbjct: 661 FYALDPSFPK-KNLLTRTQE-GKKRQLYMVSKELRNVLLHN---SEKMKVINTGIKVLCR 691

Query: 721 QSSREGSSAPCAFRISSEGLPVLLPYITKQILTMSPVDFKHLLQYKSIKYADFVDAAFGE 780
             + +G    CA+R++ EG+  L P+I  +I+T+S  D K LL  ++   + F      +
Sbjct: 721 --NNDGEQYGCAYRLAQEGIYTLYPFINARIVTVSIEDIKVLLTQENPFLSKF-SKETQK 691

Query: 781 KASNLMLGCCVIVLGTGNTSSDVIKVDESTIAIGCWKGRASLSVMV 819
           +A+N  +G  V+         + +   +  I +  W+G+ S+   V
Sbjct: 781 QANNFDMGSIVLKYEPDPQEPETL---QCPIVLCGWRGKTSIRSFV 691

BLAST of Carg15981 vs. ExPASy Swiss-Prot
Match: Q4V7N2 (RNA cytosine-C(5)-methyltransferase NSUN2 OS=Xenopus laevis OX=8355 GN=nsun2 PE=2 SV=1)

HSP 1 Score: 395.6 bits (1015), Expect = 1.4e-108
Identity = 275/816 (33.70%), Postives = 404/816 (49.51%), Query Frame = 0

Query: 5   GRGGRARTQRKHFRESRENVWKRPKPDPNSANGNKDGNGNHWEPFESQSHAFNEYYKEQG 64
           GR  R   QR+  + S     +R   +  +  G        +     ++  F  YY+E  
Sbjct: 2   GRKNRRNRQRRTEQRSPAEEERRKAREQAAWEGG-------YPEIIKENKLFEHYYQELK 61

Query: 65  IVTAEEWDTFIEFLRKPLPAAFRINSSGQFCGEIRAQLQNDFTKSLQAEVTDGDEAEAIR 124
           IV   EWD F++ LR+PLPA  RI        EI   L+  + K L     DG + EA +
Sbjct: 62  IVPDGEWDKFMDALREPLPATIRITGYKSHAKEILHCLKEKYFKELPDIEVDGQKIEAPQ 121

Query: 125 PLPWYPDNLAWHSNFSRMQLRKNQALESFHEFLKLENEIGNITRQEAVSMVPPLFLDVHP 184
           PL WYP+ LAWH+N SR  +RK+  LE FH+FL  E E GNI+RQEAVSM+PP+ L+V P
Sbjct: 122 PLSWYPEELAWHTNLSRKIIRKSPELEKFHQFLVSETESGNISRQEAVSMIPPVLLNVQP 181

Query: 185 NHYVLDMCAAPGSKTFQLLEIIHQSSKPGDLPDGLVVANDLDVQRCNLLIHQTKRMCTAN 244
           +H +LDMCAAPGSKT Q++E++H        P+G V+AND+D +RC LL+HQ KR+ +  
Sbjct: 182 HHKILDMCAAPGSKTAQIIEMLHADMNV-PFPEGFVIANDVDNKRCYLLVHQAKRLNSPC 241

Query: 245 LIVTNHEAQHFPGCRANTNFFSASTAGNELKPRSTQLTFDRVLCDVPCSGDGTLRKAPDI 304
           ++V NH+A   P      N         E+      L +DR+LCDVPCSGDGT+RK  D+
Sbjct: 242 IMVVNHDASSIPRLLIENN------GSREV------LYYDRILCDVPCSGDGTMRKNIDV 301

Query: 305 WRKWNSGMGNGLHGLQVQIGMRGASLLKVGGRMVYSTCSMNPVENEAVVAELLRKSGGSL 364
           W+KW +     LHGLQ++I  RG   L  GGRMVYSTCS+NPVE+EAV+  LL KS GSL
Sbjct: 302 WKKWTTLNSLQLHGLQIRIATRGVEQLAEGGRMVYSTCSLNPVEDEAVIVSLLDKSEGSL 361

Query: 365 ELVDVSNELPQLVRRPGLKKWKVCDK-GVWFASYEQVTESCQSVAIPGMFPSGRGRKSDT 424
           EL DV++ELP L   PG+ +W+V  K G WF  +E V  S  +   P MFP     K  +
Sbjct: 362 ELADVASELPGLKWMPGITQWRVMTKEGQWFEKWEDVPTSRHTQIRPTMFPLKDEEKLKS 421

Query: 425 HNSELRGNHPDGGSSVDVSEPTMDPVHVFDEEVCEFPIERCMRIVPHDQNTGAFFVAVLR 484
            N                                   + RCMRI+PH QNTG FFVAVL 
Sbjct: 422 MN-----------------------------------LNRCMRILPHHQNTGGFFVAVLI 481

Query: 485 KVAPLPAATEKTSDQNTKSANDQSHGKLQQTITDADSVDLNATDGMGESSSEVEIDPNNQ 544
           K AP+P               ++   KLQ+                          P + 
Sbjct: 482 KKAPMPW--------------NKRQPKLQRR------------------------PPVSV 541

Query: 545 RDSNSANNNPPCKLPVQDNKDADGLDSTDGMDKQFSEADMEVVENETTENKLEITAIKNN 604
            D++ A         +    D   +D+ +G  K  +        N++  +K +       
Sbjct: 542 CDASVAPEIVKAVADISAIADEPAVDAENGETKPCT--------NQSGSSKTDSVCCPPP 601

Query: 605 SEESKQPGDGEMDPKKCNGKRKLQIQGKWRGVDP-VVLFKDETVIDCIKTFYGINESFPL 664
           S++ K  G  E                     DP V L +D+ + + I+ FY ++ SFP 
Sbjct: 602 SKKMKLFGFKE---------------------DPFVFLSEDDPIFEPIQKFYALDPSFPK 661

Query: 665 IGHLVTRNSDTNHVKRIYYISNSVKDVLELNFSAGQQLKITSIGLKMFERQSSREGSSAP 724
             +L+TR  +    +++Y +S  +++VL  N    +++K+ + G+K+  R  + +G    
Sbjct: 662 -KNLLTRTQE-GKKRQLYMVSKELRNVLLHN---SEKMKVINTGIKVLCR--NNDGEQYG 684

Query: 725 CAFRISSEGLPVLLPYITKQILTMSPVDFKHLLQYKSIKYADFVDAAFGEKASNLMLGCC 784
           CA+R++ EG+  L P+I  +ILT+S  D K LL  ++   + F      ++A+NL +G  
Sbjct: 722 CAYRLAQEGIYSLYPFINARILTVSVEDIKVLLTQENPFLSKF-SKETQKQANNLDMGSI 684

Query: 785 VIVLGTGNTSSDVIKVDESTIAIGCWKGRASLSVMV 819
           V+         + +   +  I +  W+G+ S+   V
Sbjct: 782 VLKYEPDPQQPETL---QCPIVLCGWRGKTSIRSFV 684

BLAST of Carg15981 vs. ExPASy TrEMBL
Match: A0A6J1HGN2 (tRNA (Cytosine(34)-C(5))-methyltransferase-like OS=Cucurbita moschata OX=3662 GN=LOC111463320 PE=3 SV=1)

HSP 1 Score: 1691.0 bits (4378), Expect = 0.0e+00
Identity = 838/838 (100.00%), Postives = 838/838 (100.00%), Query Frame = 0

Query: 1   MGGKGRGGRARTQRKHFRESRENVWKRPKPDPNSANGNKDGNGNHWEPFESQSHAFNEYY 60
           MGGKGRGGRARTQRKHFRESRENVWKRPKPDPNSANGNKDGNGNHWEPFESQSHAFNEYY
Sbjct: 1   MGGKGRGGRARTQRKHFRESRENVWKRPKPDPNSANGNKDGNGNHWEPFESQSHAFNEYY 60

Query: 61  KEQGIVTAEEWDTFIEFLRKPLPAAFRINSSGQFCGEIRAQLQNDFTKSLQAEVTDGDEA 120
           KEQGIVTAEEWDTFIEFLRKPLPAAFRINSSGQFCGEIRAQLQNDFTKSLQAEVTDGDEA
Sbjct: 61  KEQGIVTAEEWDTFIEFLRKPLPAAFRINSSGQFCGEIRAQLQNDFTKSLQAEVTDGDEA 120

Query: 121 EAIRPLPWYPDNLAWHSNFSRMQLRKNQALESFHEFLKLENEIGNITRQEAVSMVPPLFL 180
           EAIRPLPWYPDNLAWHSNFSRMQLRKNQALESFHEFLKLENEIGNITRQEAVSMVPPLFL
Sbjct: 121 EAIRPLPWYPDNLAWHSNFSRMQLRKNQALESFHEFLKLENEIGNITRQEAVSMVPPLFL 180

Query: 181 DVHPNHYVLDMCAAPGSKTFQLLEIIHQSSKPGDLPDGLVVANDLDVQRCNLLIHQTKRM 240
           DVHPNHYVLDMCAAPGSKTFQLLEIIHQSSKPGDLPDGLVVANDLDVQRCNLLIHQTKRM
Sbjct: 181 DVHPNHYVLDMCAAPGSKTFQLLEIIHQSSKPGDLPDGLVVANDLDVQRCNLLIHQTKRM 240

Query: 241 CTANLIVTNHEAQHFPGCRANTNFFSASTAGNELKPRSTQLTFDRVLCDVPCSGDGTLRK 300
           CTANLIVTNHEAQHFPGCRANTNFFSASTAGNELKPRSTQLTFDRVLCDVPCSGDGTLRK
Sbjct: 241 CTANLIVTNHEAQHFPGCRANTNFFSASTAGNELKPRSTQLTFDRVLCDVPCSGDGTLRK 300

Query: 301 APDIWRKWNSGMGNGLHGLQVQIGMRGASLLKVGGRMVYSTCSMNPVENEAVVAELLRKS 360
           APDIWRKWNSGMGNGLHGLQVQIGMRGASLLKVGGRMVYSTCSMNPVENEAVVAELLRKS
Sbjct: 301 APDIWRKWNSGMGNGLHGLQVQIGMRGASLLKVGGRMVYSTCSMNPVENEAVVAELLRKS 360

Query: 361 GGSLELVDVSNELPQLVRRPGLKKWKVCDKGVWFASYEQVTESCQSVAIPGMFPSGRGRK 420
           GGSLELVDVSNELPQLVRRPGLKKWKVCDKGVWFASYEQVTESCQSVAIPGMFPSGRGRK
Sbjct: 361 GGSLELVDVSNELPQLVRRPGLKKWKVCDKGVWFASYEQVTESCQSVAIPGMFPSGRGRK 420

Query: 421 SDTHNSELRGNHPDGGSSVDVSEPTMDPVHVFDEEVCEFPIERCMRIVPHDQNTGAFFVA 480
           SDTHNSELRGNHPDGGSSVDVSEPTMDPVHVFDEEVCEFPIERCMRIVPHDQNTGAFFVA
Sbjct: 421 SDTHNSELRGNHPDGGSSVDVSEPTMDPVHVFDEEVCEFPIERCMRIVPHDQNTGAFFVA 480

Query: 481 VLRKVAPLPAATEKTSDQNTKSANDQSHGKLQQTITDADSVDLNATDGMGESSSEVEIDP 540
           VLRKVAPLPAATEKTSDQNTKSANDQSHGKLQQTITDADSVDLNATDGMGESSSEVEIDP
Sbjct: 481 VLRKVAPLPAATEKTSDQNTKSANDQSHGKLQQTITDADSVDLNATDGMGESSSEVEIDP 540

Query: 541 NNQRDSNSANNNPPCKLPVQDNKDADGLDSTDGMDKQFSEADMEVVENETTENKLEITAI 600
           NNQRDSNSANNNPPCKLPVQDNKDADGLDSTDGMDKQFSEADMEVVENETTENKLEITAI
Sbjct: 541 NNQRDSNSANNNPPCKLPVQDNKDADGLDSTDGMDKQFSEADMEVVENETTENKLEITAI 600

Query: 601 KNNSEESKQPGDGEMDPKKCNGKRKLQIQGKWRGVDPVVLFKDETVIDCIKTFYGINESF 660
           KNNSEESKQPGDGEMDPKKCNGKRKLQIQGKWRGVDPVVLFKDETVIDCIKTFYGINESF
Sbjct: 601 KNNSEESKQPGDGEMDPKKCNGKRKLQIQGKWRGVDPVVLFKDETVIDCIKTFYGINESF 660

Query: 661 PLIGHLVTRNSDTNHVKRIYYISNSVKDVLELNFSAGQQLKITSIGLKMFERQSSREGSS 720
           PLIGHLVTRNSDTNHVKRIYYISNSVKDVLELNFSAGQQLKITSIGLKMFERQSSREGSS
Sbjct: 661 PLIGHLVTRNSDTNHVKRIYYISNSVKDVLELNFSAGQQLKITSIGLKMFERQSSREGSS 720

Query: 721 APCAFRISSEGLPVLLPYITKQILTMSPVDFKHLLQYKSIKYADFVDAAFGEKASNLMLG 780
           APCAFRISSEGLPVLLPYITKQILTMSPVDFKHLLQYKSIKYADFVDAAFGEKASNLMLG
Sbjct: 721 APCAFRISSEGLPVLLPYITKQILTMSPVDFKHLLQYKSIKYADFVDAAFGEKASNLMLG 780

Query: 781 CCVIVLGTGNTSSDVIKVDESTIAIGCWKGRASLSVMVTATDCQELLGRLSNRLGNDL 839
           CCVIVLGTGNTSSDVIKVDESTIAIGCWKGRASLSVMVTATDCQELLGRLSNRLGNDL
Sbjct: 781 CCVIVLGTGNTSSDVIKVDESTIAIGCWKGRASLSVMVTATDCQELLGRLSNRLGNDL 838

BLAST of Carg15981 vs. ExPASy TrEMBL
Match: A0A6J1KLF5 (tRNA (Cytosine(34)-C(5))-methyltransferase-like OS=Cucurbita maxima OX=3661 GN=LOC111496800 PE=3 SV=1)

HSP 1 Score: 1664.4 bits (4309), Expect = 0.0e+00
Identity = 826/838 (98.57%), Postives = 831/838 (99.16%), Query Frame = 0

Query: 1   MGGKGRGGRARTQRKHFRESRENVWKRPKPDPNSANGNKDGNGNHWEPFESQSHAFNEYY 60
           MGGKGRGGRARTQRKHFRESRENVWKRPKPDPNSAN NKDGNGNHWEPFESQS AFNEYY
Sbjct: 1   MGGKGRGGRARTQRKHFRESRENVWKRPKPDPNSANDNKDGNGNHWEPFESQSPAFNEYY 60

Query: 61  KEQGIVTAEEWDTFIEFLRKPLPAAFRINSSGQFCGEIRAQLQNDFTKSLQAEVTDGDEA 120
           KEQGIVTAEEWDTFIE LRKPLPAAFRINSSGQFCGEIRAQLQNDF++SLQAEVTDGDEA
Sbjct: 61  KEQGIVTAEEWDTFIEVLRKPLPAAFRINSSGQFCGEIRAQLQNDFSRSLQAEVTDGDEA 120

Query: 121 EAIRPLPWYPDNLAWHSNFSRMQLRKNQALESFHEFLKLENEIGNITRQEAVSMVPPLFL 180
           EAIRPLPWYPDNLAWHSNFSRMQLRKNQALESFHEFLKLENEIGNITRQEAVSMVPPLFL
Sbjct: 121 EAIRPLPWYPDNLAWHSNFSRMQLRKNQALESFHEFLKLENEIGNITRQEAVSMVPPLFL 180

Query: 181 DVHPNHYVLDMCAAPGSKTFQLLEIIHQSSKPGDLPDGLVVANDLDVQRCNLLIHQTKRM 240
           DVHPNHYVLDMCAAPGSKTFQLLEIIHQSSKPGDLPDGLVVANDLDVQRCNLLIHQTKRM
Sbjct: 181 DVHPNHYVLDMCAAPGSKTFQLLEIIHQSSKPGDLPDGLVVANDLDVQRCNLLIHQTKRM 240

Query: 241 CTANLIVTNHEAQHFPGCRANTNFFSASTAGNELKPRSTQLTFDRVLCDVPCSGDGTLRK 300
           CTANLIVTNHEAQHFPGCRANTNFFSASTAGNELKPRSTQLTFDRVLCDVPCSGDGTLRK
Sbjct: 241 CTANLIVTNHEAQHFPGCRANTNFFSASTAGNELKPRSTQLTFDRVLCDVPCSGDGTLRK 300

Query: 301 APDIWRKWNSGMGNGLHGLQVQIGMRGASLLKVGGRMVYSTCSMNPVENEAVVAELLRKS 360
           APDIWRKWNSGMGNGLHGLQVQIGMRGASLLKVGGRMVYSTCSMNPVENEAVVAELLRKS
Sbjct: 301 APDIWRKWNSGMGNGLHGLQVQIGMRGASLLKVGGRMVYSTCSMNPVENEAVVAELLRKS 360

Query: 361 GGSLELVDVSNELPQLVRRPGLKKWKVCDKGVWFASYEQVTESCQSVAIPGMFPSGRGRK 420
           GGSLELVDVSNELPQLVRRPGLKKWKVCDKGVWFASYEQVTESCQSVAIPGMFPSGRG K
Sbjct: 361 GGSLELVDVSNELPQLVRRPGLKKWKVCDKGVWFASYEQVTESCQSVAIPGMFPSGRGHK 420

Query: 421 SDTHNSELRGNHPDGGSSVDVSEPTMDPVHVFDEEVCEFPIERCMRIVPHDQNTGAFFVA 480
           SDTHNS+LRGNHPDGGSSVDVSEPTMDPVHVFDEEVCEFPIERCMRIVPHDQNTGAFFVA
Sbjct: 421 SDTHNSQLRGNHPDGGSSVDVSEPTMDPVHVFDEEVCEFPIERCMRIVPHDQNTGAFFVA 480

Query: 481 VLRKVAPLPAATEKTSDQNTKSANDQSHGKLQQTITDADSVDLNATDGMGESSSEVEIDP 540
           VLRKVAPL AATEKTSDQNTKSANDQS GKLQQTITDADSVDLNATDGMGESSSEVEIDP
Sbjct: 481 VLRKVAPLLAATEKTSDQNTKSANDQSRGKLQQTITDADSVDLNATDGMGESSSEVEIDP 540

Query: 541 NNQRDSNSANNNPPCKLPVQDNKDADGLDSTDGMDKQFSEADMEVVENETTENKLEITAI 600
           NNQRDSNSANNNPPCKLPVQDNKDADGLDSTDGMDK FSEAD+EVV+NETTENKLEITAI
Sbjct: 541 NNQRDSNSANNNPPCKLPVQDNKDADGLDSTDGMDKPFSEADLEVVDNETTENKLEITAI 600

Query: 601 KNNSEESKQPGDGEMDPKKCNGKRKLQIQGKWRGVDPVVLFKDETVIDCIKTFYGINESF 660
           KNNSEESKQPGDGEMDPKKCNGKRKLQIQGKWRGVDPVVLFKDETVIDCIKTFYGINESF
Sbjct: 601 KNNSEESKQPGDGEMDPKKCNGKRKLQIQGKWRGVDPVVLFKDETVIDCIKTFYGINESF 660

Query: 661 PLIGHLVTRNSDTNHVKRIYYISNSVKDVLELNFSAGQQLKITSIGLKMFERQSSREGSS 720
           PLIGHLVTRNSDTNHVKRIYYISNSVKDVLELNFSAGQQLKITSIGLKMFERQSSREGSS
Sbjct: 661 PLIGHLVTRNSDTNHVKRIYYISNSVKDVLELNFSAGQQLKITSIGLKMFERQSSREGSS 720

Query: 721 APCAFRISSEGLPVLLPYITKQILTMSPVDFKHLLQYKSIKYADFVDAAFGEKASNLMLG 780
           APCAFRISSEGLPVLLPYITKQILTMSPVDFKHLLQYKSIKYADFVDAAFGEKASNLMLG
Sbjct: 721 APCAFRISSEGLPVLLPYITKQILTMSPVDFKHLLQYKSIKYADFVDAAFGEKASNLMLG 780

Query: 781 CCVIVLGTGNTSSDVIKVDESTIAIGCWKGRASLSVMVTATDCQELLGRLSNRLGNDL 839
           CCVIVLGTGNTSSDVIKVDESTIAIGCWKGRASLSVMVTATDCQELLGRLSNRLGNDL
Sbjct: 781 CCVIVLGTGNTSSDVIKVDESTIAIGCWKGRASLSVMVTATDCQELLGRLSNRLGNDL 838

BLAST of Carg15981 vs. ExPASy TrEMBL
Match: A0A6J1GFP9 (tRNA (Cytosine(34)-C(5))-methyltransferase-like isoform X1 OS=Cucurbita moschata OX=3662 GN=LOC111453515 PE=3 SV=1)

HSP 1 Score: 1420.6 bits (3676), Expect = 0.0e+00
Identity = 721/848 (85.02%), Postives = 767/848 (90.45%), Query Frame = 0

Query: 1   MGGKGRGGRARTQRKHFRESRENVWKRPKPDPNSANGNKDGNGNHWEPFESQSHAFNEYY 60
           MGGKGRGGRARTQR HFRESRENVWKR KPD NSAN  KDGN NHW PF+SQSHAFNEYY
Sbjct: 1   MGGKGRGGRARTQRNHFRESRENVWKRSKPDSNSANDTKDGNSNHWGPFQSQSHAFNEYY 60

Query: 61  KEQGIVTAEEWDTFIEFLRKPLPAAFRINSSGQFCGEIRAQLQNDFTKSLQAEVTDGDEA 120
           KEQGIVTA EWDTFIE LRK LPAAFRINSSGQFCGEIRAQLQNDF KSLQ+EVTDGDEA
Sbjct: 61  KEQGIVTAAEWDTFIEVLRKQLPAAFRINSSGQFCGEIRAQLQNDFMKSLQSEVTDGDEA 120

Query: 121 EAIRPLPWYPDNLAWHSNFSRMQLRKNQALESFHEFLKLENEIGNITRQEAVSMVPPLFL 180
            AIRPL WYPDNLAWHSNFSRMQLRKNQALE FHEFLKLENEIGNITRQEAVSMVPPLFL
Sbjct: 121 GAIRPLSWYPDNLAWHSNFSRMQLRKNQALERFHEFLKLENEIGNITRQEAVSMVPPLFL 180

Query: 181 DVHPNHYVLDMCAAPGSKTFQLLEIIHQSSKPGDLPDGLVVANDLDVQRCNLLIHQTKRM 240
           DVHPNHYVLDMCAAPGSKTFQLLEIIHQSSKPGDLP GLVVAND DVQRCNLLIHQTKRM
Sbjct: 181 DVHPNHYVLDMCAAPGSKTFQLLEIIHQSSKPGDLPGGLVVANDSDVQRCNLLIHQTKRM 240

Query: 241 CTANLIVTNHEAQHFPGCRANTNFFSASTAGNELKPRSTQLTFDRVLCDVPCSGDGTLRK 300
           CT+NLIVTNHEAQHFPGCRA+TN F+AS AG+EL P +TQLTFDRVLCDVPCSGDGTLRK
Sbjct: 241 CTSNLIVTNHEAQHFPGCRAHTNIFNASAAGSELNPHNTQLTFDRVLCDVPCSGDGTLRK 300

Query: 301 APDIWRKWNSGMGNGLHGLQVQIGMRGASLLKVGGRMVYSTCSMNPVENEAVVAELLRKS 360
           APDIWR+WNSG+GNGLHGLQVQIGMRGASLLKVGGRMVYSTCSMNPVENEAVVAELLRKS
Sbjct: 301 APDIWRRWNSGVGNGLHGLQVQIGMRGASLLKVGGRMVYSTCSMNPVENEAVVAELLRKS 360

Query: 361 GGSLELVDVSNELPQLVRRPGLKKWKVCDKGVWFASYEQVTESCQSVAIPGMFPSGRGRK 420
            GSLELVDVSNELPQLVRRPGLKKWKV D+GVW ASY+QV++  QSVAIP MFPSGRGRK
Sbjct: 361 EGSLELVDVSNELPQLVRRPGLKKWKVRDRGVWLASYKQVSDHRQSVAIPSMFPSGRGRK 420

Query: 421 SD---THNSELRGNHPDG--GSSVDVSEPTMDPVHVFDEEVCEFPIERCMRIVPHDQNTG 480
           +    +HNSELRGNH DG  GSSVDVSEPTMDPVH FD EVCEFPIERCMRIVPHDQN+G
Sbjct: 421 NQADKSHNSELRGNHLDGSIGSSVDVSEPTMDPVHEFD-EVCEFPIERCMRIVPHDQNSG 480

Query: 481 AFFVAVLRKVAPLPAATEKTSDQNTKSANDQSHGKLQQTITDADSVDLNATDGMGESSSE 540
           AFF+AVLRKVAPLPAATEK S QN K  ND+S  KL QTIT+  +VD++A+ GM ES SE
Sbjct: 481 AFFIAVLRKVAPLPAATEKPSYQNLKPVNDKSRCKLPQTITNGANVDVSASVGMDESPSE 540

Query: 541 VEIDPNNQRDSNSANNNPPCKLPVQDNKDADGLDS--TDGMDKQFSEADMEV--VENETT 600
           V +D NNQ D NSA+N P C+ PVQD +  +GLDS   DG+DKQFSEAD+EV  V+NE +
Sbjct: 541 VGVDSNNQGDFNSADNKPACEFPVQDIEGTEGLDSNAADGIDKQFSEADLEVNLVDNEVS 600

Query: 601 ENKLEITAIKNNSEESKQPGDGEMDPKKCNGKRKLQIQGKWRGVDPVVLFKDETVIDCIK 660
            N LEITAI++N EESKQP DGEMDPKK   KRKLQIQGKW+GVDPVVLF+DETVID IK
Sbjct: 601 VNNLEITAIEDNLEESKQPDDGEMDPKKSTVKRKLQIQGKWKGVDPVVLFRDETVIDSIK 660

Query: 661 TFYGINESFPLIGHLVTRNSDTNHVKRIYYISNSVKDVLELNFSAGQQLKITSIGLKMFE 720
           TFYGINE+FPL GHLVTRN+DTNHVKRIYY+S SVKDVLELNF  GQQLKITSIGLKMFE
Sbjct: 661 TFYGINEAFPLFGHLVTRNTDTNHVKRIYYVSKSVKDVLELNFLVGQQLKITSIGLKMFE 720

Query: 721 RQSSREGSSAPCAFRISSEGLPVLLPYITKQILTMSPVDFKHLLQYKSIKYADFVDAAFG 780
           RQSSREGS APC+FRI+SEGLPV+LPYITKQILTMSPVDFKHL+Q+KSIKYADF DAAFG
Sbjct: 721 RQSSREGSCAPCSFRITSEGLPVILPYITKQILTMSPVDFKHLIQHKSIKYADFADAAFG 780

Query: 781 EKASNLMLGCCVIVLGTG-NTSSDVIKVDESTIAIGCWKGRASLSVMVTATDCQELLGRL 839
           EKASNLMLGCCVIVLGTG NTSSDVIKVDESTIAIGCWKGR+SLSVMVTA DCQELL RL
Sbjct: 781 EKASNLMLGCCVIVLGTGSNTSSDVIKVDESTIAIGCWKGRSSLSVMVTAIDCQELLERL 840

BLAST of Carg15981 vs. ExPASy TrEMBL
Match: A0A6J1IQ20 (tRNA (Cytosine(34)-C(5))-methyltransferase-like isoform X1 OS=Cucurbita maxima OX=3661 GN=LOC111478413 PE=3 SV=1)

HSP 1 Score: 1417.1 bits (3667), Expect = 0.0e+00
Identity = 720/848 (84.91%), Postives = 767/848 (90.45%), Query Frame = 0

Query: 1   MGGKGRGGRARTQRKHFRESRENVWKRPKPDPNSANGNKDGNGNHWEPFESQSHAFNEYY 60
           MGGKGRGGRARTQR HFRESRENVWKR KPD NSAN  KDGN NHW PF+SQSHAFNEYY
Sbjct: 1   MGGKGRGGRARTQRNHFRESRENVWKRSKPDSNSANDTKDGNSNHWGPFQSQSHAFNEYY 60

Query: 61  KEQGIVTAEEWDTFIEFLRKPLPAAFRINSSGQFCGEIRAQLQNDFTKSLQAEVTDGDEA 120
           KEQGIVTAEEWDTFIE LRK LPAAFRINSSGQFCGEIRAQLQNDF KSLQ+EVTDGDEA
Sbjct: 61  KEQGIVTAEEWDTFIEVLRKQLPAAFRINSSGQFCGEIRAQLQNDFMKSLQSEVTDGDEA 120

Query: 121 EAIRPLPWYPDNLAWHSNFSRMQLRKNQALESFHEFLKLENEIGNITRQEAVSMVPPLFL 180
            AIRPL WYPDNLAWHSNFSRMQLRKNQALE FHEFLKLENEIGNITRQEAVSMVPPLFL
Sbjct: 121 GAIRPLSWYPDNLAWHSNFSRMQLRKNQALERFHEFLKLENEIGNITRQEAVSMVPPLFL 180

Query: 181 DVHPNHYVLDMCAAPGSKTFQLLEIIHQSSKPGDLPDGLVVANDLDVQRCNLLIHQTKRM 240
           DVHPNHYVLDMCAAPGSKTFQLLEIIHQSSKPGDLP GLVVAND DVQRCNLLIHQTKRM
Sbjct: 181 DVHPNHYVLDMCAAPGSKTFQLLEIIHQSSKPGDLPGGLVVANDSDVQRCNLLIHQTKRM 240

Query: 241 CTANLIVTNHEAQHFPGCRANTNFFSASTAGNELKPRSTQLTFDRVLCDVPCSGDGTLRK 300
           CT+NLIVTNHEAQHFPGCRA+TNFF+AS AG+EL   +TQLTFDRVLCDVPCSGDGTLRK
Sbjct: 241 CTSNLIVTNHEAQHFPGCRAHTNFFNASAAGSELNSHNTQLTFDRVLCDVPCSGDGTLRK 300

Query: 301 APDIWRKWNSGMGNGLHGLQVQIGMRGASLLKVGGRMVYSTCSMNPVENEAVVAELLRKS 360
           APDIWR+WNSG+GNGLHGLQVQIGMRGASLLKVGGRMVYSTCSMNPVENEAVVAELLRKS
Sbjct: 301 APDIWRRWNSGVGNGLHGLQVQIGMRGASLLKVGGRMVYSTCSMNPVENEAVVAELLRKS 360

Query: 361 GGSLELVDVSNELPQLVRRPGLKKWKVCDKGVWFASYEQVTESCQSVAIPGMFPSGRGRK 420
            GSLE+VDVSNELPQLVRRPGLKKWKV D+GVW ASY+QV++  QSVAIP MFP GRGRK
Sbjct: 361 EGSLEVVDVSNELPQLVRRPGLKKWKVRDRGVWLASYKQVSDHRQSVAIPSMFPFGRGRK 420

Query: 421 SD---THNSELRGNHPDG--GSSVDVSEPTMDPVHVFDEEVCEFPIERCMRIVPHDQNTG 480
           +    +HNSELRGNH +G  GSSVDVSEPTMDPVH FD EVCEFPIERCMRIVPHDQN+G
Sbjct: 421 NQADKSHNSELRGNHLNGSIGSSVDVSEPTMDPVHEFD-EVCEFPIERCMRIVPHDQNSG 480

Query: 481 AFFVAVLRKVAPLPAATEKTSDQNTKSANDQSHGKLQQTITDADSVDLNATDGMGESSSE 540
           AFF+AVLRKVAPLPAATEK S  N K AND+S  KL Q IT+  +VD++A+ GM ES SE
Sbjct: 481 AFFIAVLRKVAPLPAATEKPSYHNLKPANDKSRCKLPQNITNGANVDVSASVGMDESPSE 540

Query: 541 VEIDPNNQRDSNSANNNPPCKLPVQDNKDADGLDS--TDGMDKQFSEADMEV--VENETT 600
           V ID NNQ D NSA+N P C+ PVQD +  +GLDS   DG+DKQFSEAD+EV  ++NE  
Sbjct: 541 VGIDSNNQGDFNSADNKPACEFPVQDIEGKEGLDSNAADGIDKQFSEADLEVNLIDNEVN 600

Query: 601 ENKLEITAIKNNSEESKQPGDGEMDPKKCNGKRKLQIQGKWRGVDPVVLFKDETVIDCIK 660
            N LEITAI++N EESKQP DGEMDPKK   KRKLQIQGKW+GVDPVVLFKDETVID IK
Sbjct: 601 VNNLEITAIEDNLEESKQPDDGEMDPKKSTVKRKLQIQGKWKGVDPVVLFKDETVIDSIK 660

Query: 661 TFYGINESFPLIGHLVTRNSDTNHVKRIYYISNSVKDVLELNFSAGQQLKITSIGLKMFE 720
           TFYGINE+FPL GHLVTRN+DTNHVKRIYY+S SVKDVLELNFS GQQLKITSIGLKMFE
Sbjct: 661 TFYGINEAFPLFGHLVTRNTDTNHVKRIYYVSKSVKDVLELNFSVGQQLKITSIGLKMFE 720

Query: 721 RQSSREGSSAPCAFRISSEGLPVLLPYITKQILTMSPVDFKHLLQYKSIKYADFVDAAFG 780
           RQSSREGSSAPC+FRI+SEGLPV+LPYITKQILTMSPVDFKHL+Q+KSIKYADF DAAFG
Sbjct: 721 RQSSREGSSAPCSFRITSEGLPVILPYITKQILTMSPVDFKHLIQHKSIKYADFADAAFG 780

Query: 781 EKASNLMLGCCVIVLGT-GNTSSDVIKVDESTIAIGCWKGRASLSVMVTATDCQELLGRL 839
           EKAS+LMLGCCVIVLGT GNTSSDVIKVDESTIAIGCWKGR+SLSVMVTA DCQELL RL
Sbjct: 781 EKASSLMLGCCVIVLGTGGNTSSDVIKVDESTIAIGCWKGRSSLSVMVTAIDCQELLERL 840

BLAST of Carg15981 vs. ExPASy TrEMBL
Match: A0A6J1BXA3 (tRNA (Cytosine(34)-C(5))-methyltransferase-like OS=Momordica charantia OX=3673 GN=LOC111005554 PE=3 SV=1)

HSP 1 Score: 1403.7 bits (3632), Expect = 0.0e+00
Identity = 717/850 (84.35%), Postives = 763/850 (89.76%), Query Frame = 0

Query: 1   MGGKGRGGRARTQRKHFRESRENVWKRPKPDPNSANGNKDGNG--NHWEPFESQSHAFNE 60
           MGGKGRGGRARTQRKHFRE+RENVWKRP+PDPNS N NKDGNG  N WEPF+SQS AFNE
Sbjct: 1   MGGKGRGGRARTQRKHFRENRENVWKRPRPDPNSGNDNKDGNGNTNPWEPFDSQSPAFNE 60

Query: 61  YYKEQGIVTAEEWDTFIEFLRKPLPAAFRINSSGQFCGEIRAQLQNDFTKSLQAEVTDGD 120
           YYKEQGIVTAEEWDTFIE LRKPLPAAFRINSSGQFCGEIRAQLQNDF KSL+AEV DGD
Sbjct: 61  YYKEQGIVTAEEWDTFIEVLRKPLPAAFRINSSGQFCGEIRAQLQNDFMKSLKAEVADGD 120

Query: 121 EAEAIRPLPWYPDNLAWHSNFSRMQLRKNQALESFHEFLKLENEIGNITRQEAVSMVPPL 180
           EAEAIRPLPWYP+NLAWHSNFSRMQLRKNQALE FHEFLKLENEIGNITRQEAVSMVPPL
Sbjct: 121 EAEAIRPLPWYPENLAWHSNFSRMQLRKNQALERFHEFLKLENEIGNITRQEAVSMVPPL 180

Query: 181 FLDVHPNHYVLDMCAAPGSKTFQLLEIIHQSSKPGDLPDGLVVANDLDVQRCNLLIHQTK 240
           FLDVHP+HYVLDMCAAPGSKTFQLLEIIH SSKPG LP+GLVVANDLDVQRCNLLIHQTK
Sbjct: 181 FLDVHPSHYVLDMCAAPGSKTFQLLEIIHLSSKPGGLPEGLVVANDLDVQRCNLLIHQTK 240

Query: 241 RMCTANLIVTNHEAQHFPGCRANTNFFSASTAGNELKPRSTQLTFDRVLCDVPCSGDGTL 300
           RMCTANLIVTNHEAQHFPGCRAN NFF+AS AGNELKP+++QL+FDRVLCDVPCSGDGTL
Sbjct: 241 RMCTANLIVTNHEAQHFPGCRANINFFNASAAGNELKPQNSQLSFDRVLCDVPCSGDGTL 300

Query: 301 RKAPDIWRKWNSGMGNGLHGLQVQIGMRGASLLKVGGRMVYSTCSMNPVENEAVVAELLR 360
           RKAPDIWRKWN+GMGNGLHGLQVQIGMRGASLLKVGGRMVYSTCSMNPVENEAVVAELLR
Sbjct: 301 RKAPDIWRKWNAGMGNGLHGLQVQIGMRGASLLKVGGRMVYSTCSMNPVENEAVVAELLR 360

Query: 361 KSGGSLELVDVSNELPQLVRRPGLKKWKVCDKGVWFASYEQVTESCQSVAIPGMFPSGRG 420
           KS GSLELVDVSNELPQLVRR GLKKW+V DKGVW ASY+QV E  QSV IP MFPSGR 
Sbjct: 361 KSQGSLELVDVSNELPQLVRRSGLKKWRVRDKGVWLASYKQVNECRQSVVIPSMFPSGRS 420

Query: 421 RKS---DTHNSELRGNHPDG--GSSVDVSEPTMDPVHVFDEEVCEFPIERCMRIVPHDQN 480
           RK+   D HNSEL     DG  GSSVDV++  +DP+H FDEEVCEFP+ERCMRIVPHDQN
Sbjct: 421 RKNQAEDNHNSELGETRLDGSTGSSVDVADAAVDPLHEFDEEVCEFPLERCMRIVPHDQN 480

Query: 481 TGAFFVAVLRKVAPLPAATEKTSDQNTKSANDQSHGKLQQTITDADSVDLNATDGMGESS 540
           +GAFF+AVL KV+PLPAATEK  +Q +KSAND+SH KL Q ITD DSVD+NA DGM ES 
Sbjct: 481 SGAFFIAVLHKVSPLPAATEKPINQKSKSANDRSHCKLPQAITDVDSVDINAIDGMDESP 540

Query: 541 SEVEIDPNNQRDSNSANNNPPCKLPVQDNKDADGLDST--DGMDKQFSEADME--VVENE 600
           SEVEID N Q D NSA+   P K PV+D KD DGLDS+  DGMDKQ SEA +E  +++NE
Sbjct: 541 SEVEIDLNKQGDYNSADEKSPSKRPVEDVKDDDGLDSSAADGMDKQLSEAGLEDNLIDNE 600

Query: 601 TTENKLEITAIKNNSEESKQPGDGEMDPKKCNGKRKLQIQGKWRGVDPVVLFKDETVIDC 660
            +EN LEITAIK+ +EESK+  DG  DP K   KRKLQIQGKW+GVDPVVLFKDETVID 
Sbjct: 601 VSENNLEITAIKDKAEESKEADDGGTDPNKSTVKRKLQIQGKWKGVDPVVLFKDETVIDS 660

Query: 661 IKTFYGINESFPLIGHLVTRNSDTNHVKRIYYISNSVKDVLELNFSAGQQLKITSIGLKM 720
           IKTFYGINESFPLIGHLVTRNSDTNHVKRIYYIS SVKDVLELNFS GQQLKITSIGLKM
Sbjct: 661 IKTFYGINESFPLIGHLVTRNSDTNHVKRIYYISKSVKDVLELNFSVGQQLKITSIGLKM 720

Query: 721 FERQSSREGSSAPCAFRISSEGLPVLLPYITKQILTMSPVDFKHLLQYKSIKYADFVDAA 780
           FERQSSREGSSAPC+FRISSEGLPV+LPYITKQILT+SPVDFKHLL Y+SIKYADFVDAA
Sbjct: 721 FERQSSREGSSAPCSFRISSEGLPVILPYITKQILTISPVDFKHLLIYESIKYADFVDAA 780

Query: 781 FGEKASNLMLGCCVIVLGTG-NTSSDVIKVDESTIAIGCWKGRASLSVMVTATDCQELLG 839
           FGEKASNLMLGCCVIVLG G N SSDVIKVDESTIAIGCWKGRASLSVM+TA D QELLG
Sbjct: 781 FGEKASNLMLGCCVIVLGKGSNPSSDVIKVDESTIAIGCWKGRASLSVMLTAIDRQELLG 840

BLAST of Carg15981 vs. TAIR 10
Match: AT2G22400.1 (S-adenosyl-L-methionine-dependent methyltransferases superfamily protein )

HSP 1 Score: 915.2 bits (2364), Expect = 3.8e-266
Identity = 490/847 (57.85%), Postives = 595/847 (70.25%), Query Frame = 0

Query: 5   GRGG--RARTQRKHFRESRENVWKRPKPDPNSANGNKDGNGN-------HWEPFESQSHA 64
           GRGG  R RTQRK F+ESRENVWKRPK D      + DG+ N        WEP  + +  
Sbjct: 2   GRGGRHRGRTQRKDFKESRENVWKRPKSD-----ASVDGSDNAVPEQKPTWEPIVTVNPN 61

Query: 65  FNEYYKEQGIVTAEEWDTFIEFLRKPLPAAFRINSSGQFCGEIRAQLQNDFTKSLQAEVT 124
           F EYYK+QGIV AEEWD F+E LRKPLPAAFR+NS+GQFC EI + L+NDF KSLQAE  
Sbjct: 62  FEEYYKKQGIVKAEEWDLFMEILRKPLPAAFRVNSNGQFCDEIISILENDFMKSLQAEAI 121

Query: 125 DGDEAEAIRPLPWYPDNLAWHSNFSRMQLRKNQALESFHEFLKLENEIGNITRQEAVSMV 184
           +G E EAI+PLPWYP NLAWHSNFSR ++RKNQ LE FHEFLKLENE+GNITRQEAVSMV
Sbjct: 122 EGGELEAIKPLPWYPKNLAWHSNFSRKEIRKNQTLERFHEFLKLENEVGNITRQEAVSMV 181

Query: 185 PPLFLDVHPNHYVLDMCAAPGSKTFQLLEIIHQSSKPGDLPDGLVVANDLDVQRCNLLIH 244
           PPLFLDVHP+H+VLDMCAAPGSKTFQLLEIIH++S+PG LP+GLVVAND+D +R NLLIH
Sbjct: 182 PPLFLDVHPDHFVLDMCAAPGSKTFQLLEIIHEASEPGSLPNGLVVANDVDFKRSNLLIH 241

Query: 245 QTKRMCTANLIVTNHEAQHFPGCRANTNFFSASTAGNELKPRSTQLTFDRVLCDVPCSGD 304
           QTKRMCT+NLIVTNHE Q FPGCR N +   AS  G        QL FDRVLCDVPCSGD
Sbjct: 242 QTKRMCTSNLIVTNHEGQQFPGCRLNKS--RASEKGISENMPINQLAFDRVLCDVPCSGD 301

Query: 305 GTLRKAPDIWRKWNSGMGNGLHGLQVQIGMRGASLLKVGGRMVYSTCSMNPVENEAVVAE 364
           GTLRKAPDIWRKWNSGMGNGLH LQ+ + MRG SLLKVGG+M+YSTCSMNPVE+EAVVAE
Sbjct: 302 GTLRKAPDIWRKWNSGMGNGLHSLQIILAMRGLSLLKVGGKMIYSTCSMNPVEDEAVVAE 361

Query: 365 LLRKSGGSLELVDVSNELPQLVRRPGLKKWKVCDKGVWFASYEQVTESCQSVAIPGMFPS 424
           +LR+ G S+EL+DVS++LP+L+RRPGLK WKV DKG WF SY+ V ++ +   +  MFPS
Sbjct: 362 ILRRCGDSVELLDVSDKLPELIRRPGLKAWKVRDKGGWFTSYKDVPQNRRGGVLVSMFPS 421

Query: 425 GRGRKSDTHNSELRGNH-----PDGGSSVDVSEPTMDPVHVFDEEVCEFPIERCMRIVPH 484
           G+  K  T  +E   N       DG    D+S     P     EEV + P+ERCMRI+PH
Sbjct: 422 GKYLKDSTETTEKNENGDVNGCEDGLKETDISVVDATPEEQ-AEEVSDLPLERCMRIIPH 481

Query: 485 DQNTGAFFVAVLRKVAPLPAATEKTSDQNTKSANDQSHGKLQQTITDADSVDLNATDGMG 544
           DQNTGAFF+AVL+K +PLP   EK                                    
Sbjct: 482 DQNTGAFFIAVLQKKSPLPEFQEK------------------------------------ 541

Query: 545 ESSSEVEIDPNNQRDSNSANNNPPCKLPVQDN---KDADGLDSTDGMDKQFSEADMEVVE 604
                    PN +R+S + + +   K P +++    DA G+     ++K   EAD  + +
Sbjct: 542 ---------PNTKRNSTAKSTDSTEKSPSKESVVTVDA-GVPDESAVEKVI-EADSNIEK 601

Query: 605 NETTENKLEITAIKNNSEESKQPGDGEMDPKKCNGKRKLQIQGKWRGVDPVVLFKDETVI 664
           N++ E + +IT  ++ +E      D E +     GKRK+ +QGKW+G DPVV  KDETVI
Sbjct: 602 NDSLEPEKKITEGESITE------DKEANSSNAGGKRKVPMQGKWKGFDPVVFVKDETVI 661

Query: 665 DCIKTFYGI-NESFPLIGHLVTRNSDTNHVKRIYYISNSVKDVLELNFSAGQQLKITSIG 724
           + IK FYGI +ESFPL GHLV RN+DT+ VKRIYY+S SVK+VL+LNF+ GQQLKI S+G
Sbjct: 662 NGIKEFYGIKDESFPLHGHLVARNTDTSSVKRIYYVSKSVKEVLQLNFAVGQQLKIASVG 721

Query: 725 LKMFERQSSREGSSAPCAFRISSEGLPVLLPYITKQILTMSPVDFKHLLQYKSIKYADFV 784
           LKMFERQS++EGSS  C FRISSEGLPV+LPYITKQ+L     DFK LLQ KSIK+ DFV
Sbjct: 722 LKMFERQSAKEGSSTLCPFRISSEGLPVILPYITKQVLYTPMADFKLLLQDKSIKFHDFV 781

Query: 785 DAAFGEKASNLMLGCCVIVLGTGNTSSDVIKVDESTIAIGCWKGRASLSVMVTATDCQEL 834
           +    +KA++L++G CV++L  G      +K D STIAI CW+G+ASL+VM T  DCQEL
Sbjct: 782 NPQLAQKATDLVMGSCVVILSDGEVP---VKADASTIAISCWRGKASLAVMATVADCQEL 784

BLAST of Carg15981 vs. TAIR 10
Match: AT4G40000.1 (S-adenosyl-L-methionine-dependent methyltransferases superfamily protein )

HSP 1 Score: 821.6 bits (2121), Expect = 5.7e-238
Identity = 456/842 (54.16%), Postives = 571/842 (67.81%), Query Frame = 0

Query: 3   GKGRGGRART-QRKHFRESRENVWKRPKPDPNSANGNKDGNGNHWEPFESQSHAFNEYYK 62
           G+GR  R  T +R     S ++  ++P     S + N     N  +P  S  H F EYYK
Sbjct: 2   GRGRRHRGSTSKRNQIENSNDDNVRKPLKSEASFDSN-----NLQKPICSNLH-FEEYYK 61

Query: 63  EQGIVTAEEWDTFIEFLRKPLPAAFRINSSGQFCGEIRAQLQNDFTKSLQAEVTDGDEAE 122
           EQ IV A+EWD+F+EFLRKPLPAAFR+ S+GQFC EIR +L+NDF  SLQAE  +  E E
Sbjct: 62  EQRIVKAQEWDSFMEFLRKPLPAAFRVYSNGQFCDEIRLKLENDFMISLQAEAIESGELE 121

Query: 123 AIRPLPWYPDNLAWHSNFSRMQLRKNQALESFHEFLKLENEIGNITRQEAVSMVPPLFLD 182
           AI+PLPWYP NLAWHSNFSR ++RKNQ LE FHEFLKLE E GN+TRQE+VSMVPPLFLD
Sbjct: 122 AIKPLPWYPKNLAWHSNFSRKEIRKNQTLERFHEFLKLETEAGNMTRQESVSMVPPLFLD 181

Query: 183 VHPNHYVLDMCAAPGSKTFQLLEIIHQSSKPGDLPDGLVVANDLDVQRCNLLIHQTKRMC 242
           VHP+H+VLDMCAAPGSKTFQLLEIIH+SS+PG LP+G+VVAND+D +R NLLIHQTKR C
Sbjct: 182 VHPDHFVLDMCAAPGSKTFQLLEIIHESSEPGSLPNGMVVANDVDYKRSNLLIHQTKRTC 241

Query: 243 TANLIVTNHEAQHFPGCRANTNFFSASTAGNELKPRS-TQLTFDRVLCDVPCSGDGTLRK 302
           T NL+VTN+E QHFP C        AS    E  P    QL FDRVLCDVPCSGDGTLRK
Sbjct: 242 TTNLMVTNNEGQHFPSCNTKRTLSVAS----ETNPHPIDQLLFDRVLCDVPCSGDGTLRK 301

Query: 303 APDIWRKWNSGMGNGLHGLQVQIGMRGASLLKVGGRMVYSTCSMNPVENEAVVAELLRKS 362
           APDIWR+WNSG GNGLH LQV + MRG SLLKVGGRMVYSTCSMNP+E+EAVVAE+LR+ 
Sbjct: 302 APDIWRRWNSGSGNGLHSLQVVLAMRGLSLLKVGGRMVYSTCSMNPIEDEAVVAEILRRC 361

Query: 363 GGSLELVDVSNELPQLVRRPGLKKWKVCDKGVWFASYEQVTESCQSVAIPGMFPSGRGRK 422
           G S+ELVDVS++LP+L+RRPGL KWKV D+G W+ SY+ V +S +   +  MFPSG   K
Sbjct: 362 GCSVELVDVSDKLPELIRRPGLTKWKVHDRGGWYRSYKDVPKSQRDGVLRSMFPSG---K 421

Query: 423 SDTHNSELRGNHPDGGS-SVDVSEPTMDPVHVFDEEVCEFPIERCMRIVPHDQNTGAFFV 482
           SD  +S  + ++ +  S S D S           EEVC+ P+E CMRI+PHDQNTG FF+
Sbjct: 422 SDKDSSGGKNSYEEMASISSDESA----------EEVCDLPLELCMRILPHDQNTGGFFI 481

Query: 483 AVLRKVAPLPAATEKTSDQNTKSANDQSHGKLQQTITDA--DSVDLNATDGMGESSSEVE 542
           AVL KV+PLP   EK + +   S  +  +   + +  +A  D+V     +G  E   E  
Sbjct: 482 AVLHKVSPLPDFQEKVTQKRNLSTRNNINSSEKSSSYEAVSDTVVTKPEEGTEEIVLEAT 541

Query: 543 IDPNNQRDSNSANNNPPCKLPVQDNKDADGLDSTDGMDKQFSEADMEVVENETTENKLEI 602
           +  N           PP                   M+  ++E  +E+ +      KL+ 
Sbjct: 542 VSEN--------GFEPP------------------EMESTWNEGIVELAQ------KLD- 601

Query: 603 TAIKNNSEESKQPGDGEMDPKKCNGKRKL-QIQGKWRGVDPVVLFKDETVIDCIKTFYGI 662
                                   GKR++  +QGKW+G+ PVV  +D+T I+ IKTFYGI
Sbjct: 602 ---------------------DIEGKREVPSMQGKWKGLYPVVFLRDQTGINGIKTFYGI 661

Query: 663 -NESFPLIGHLVTRNSDTN---HVKRIYYISNSVKDVLELNFSAGQQLKITSIGLKMFER 722
            +ESFPL GHLVTRNSD +   +VKRIYY+S +VK+VLELNF+ G+ LKI+S+GLKMFE+
Sbjct: 662 KDESFPLYGHLVTRNSDISSHGNVKRIYYVSKAVKNVLELNFAVGKPLKISSVGLKMFEK 721

Query: 723 QSSREGSSAPCAFRISSEGLPVLLPYITKQILTMSPVDFKHLLQYKSIKYADFVDAAFGE 782
           QS++E  +  C+FRI+SEGLPV+LPY+TKQIL  +  DFK+LLQ+KSIK+ DF+    GE
Sbjct: 722 QSAKECEANCCSFRITSEGLPVILPYMTKQILYATMADFKNLLQHKSIKFLDFIHPQIGE 766

Query: 783 KASNLMLGCCVIVL-GTGNTSSDVIKVDESTIAIGCWKGRASLSVMVTATDCQELLGRLS 834
           KA+ L LG CV+VL       S+  KV+ STIAIGCWKG+ASL+VMVT  DC +L+ RLS
Sbjct: 782 KAAELALGSCVMVLVDDSQLGSEQFKVNSSTIAIGCWKGKASLTVMVTTVDCHQLIQRLS 766

BLAST of Carg15981 vs. TAIR 10
Match: AT5G55920.1 (S-adenosyl-L-methionine-dependent methyltransferases superfamily protein )

HSP 1 Score: 83.2 bits (204), Expect = 1.1e-15
Identity = 61/195 (31.28%), Postives = 85/195 (43.59%), Query Frame = 0

Query: 169 QEAVSMVPPLFLDVHPNHYVLDMCAAPGSKTFQLLEIIHQSSKPGDLPDGLVVANDLDVQ 228
           Q A S +P + L    N  ++D+ AAPG KT  +  ++  +        GL+ AN++ V 
Sbjct: 344 QGASSFLPVMALAPRENERIVDVAAAPGGKTTYIAALMKNT--------GLIYANEMKVP 403

Query: 229 RCNLLIHQTKRMCTANLIVTNHEAQHFPGCRANTNFFSASTAGNELKPRSTQLTFDRVLC 288
           R   L     RM   N IV N++                   G EL     Q T DRVL 
Sbjct: 404 RLKSLTANLHRMGVTNTIVCNYD-------------------GRELPKVLGQNTVDRVLL 463

Query: 289 DVPCSGDGTLRKAPDIWRKWNSGMGNGLHGLQVQIGMRGASLL----KVGGRMVYSTCSM 348
           D PCSG G + K   +              LQ Q+ +    ++    K GG +VYSTCS+
Sbjct: 464 DAPCSGTGIISKDESVKITKTMDEIKKFAHLQKQLLLAAIDMVDANSKTGGYIVYSTCSI 511

Query: 349 NPVENEAVVAELLRK 360
              ENEAV+   L+K
Sbjct: 524 MVTENEAVIDYALKK 511

BLAST of Carg15981 vs. TAIR 10
Match: AT4G26600.1 (S-adenosyl-L-methionine-dependent methyltransferases superfamily protein )

HSP 1 Score: 80.5 bits (197), Expect = 7.1e-15
Identity = 60/194 (30.93%), Postives = 85/194 (43.81%), Query Frame = 0

Query: 169 QEAVSMVPPLFLDVHPNHYVLDMCAAPGSKTFQLLEIIHQSSKPGDLPDGLVVANDLDVQ 228
           Q A S +P + L       V+DM AAPG KT  +  ++  +        G++ AN++ V 
Sbjct: 327 QSASSFLPVMALAPREKERVVDMAAAPGGKTTYVAALMKNT--------GIIYANEMKVP 386

Query: 229 RCNLLIHQTKRMCTANLIVTNHEAQHFPGCRANTNFFSASTAGNELKPRSTQLTFDRVLC 288
           R   L     RM   N IV N++                   G EL     Q + DRVL 
Sbjct: 387 RLKSLSANLHRMGVTNTIVCNYD-------------------GRELTKVLGQSSVDRVLL 446

Query: 289 DVPCSGDGTLRKAPDIWRKWNSGMGNGLHGLQVQIGMRGASLL----KVGGRMVYSTCSM 348
           D PCSG G + K   +    ++        LQ Q+ +    L+    K GG +VYSTCS+
Sbjct: 447 DAPCSGTGVISKDESVKTSKSADDIKKFAHLQKQLILGAIDLVDANSKTGGYIVYSTCSV 493

Query: 349 NPVENEAVVAELLR 359
              ENEAV+   L+
Sbjct: 507 MIPENEAVIDYALK 493

BLAST of Carg15981 vs. TAIR 10
Match: AT3G13180.1 (NOL1/NOP2/sun family protein / antitermination NusB domain-containing protein )

HSP 1 Score: 76.6 bits (187), Expect = 1.0e-13
Identity = 78/266 (29.32%), Postives = 121/266 (45.49%), Query Frame = 0

Query: 104 NDFTKSLQA----EVTDGDEAEAIRPLPWYPDNLAWH-SNFSRMQLRKNQALESFHEFLK 163
           ND   SL+A    ++T  D  E +  L   P  L+ H   F R++      L++  +   
Sbjct: 252 NDPGFSLRANTGRDITRADLVERLNSLK-VPHELSLHLEEFVRIK----TGLQTVVQAGL 311

Query: 164 LENEIGNITRQEA---VSMVPPLFLDVHPNHYVLDMCAAPGSKTFQLLEIIHQSSKPGDL 223
           L+  I ++  + A   VS+V P      P   ++D CAAPG KT  +   +         
Sbjct: 312 LKEGICSVQDESAGLIVSVVKP-----QPGERIMDACAAPGGKTLFMASCLK-------- 371

Query: 224 PDGLVVANDLDVQRCNLLIHQTKRMCTANLIVTNHEAQHFPGCRANTNFFSASTAGNELK 283
             G++ A D++  R  +L    K      LI T H         ++   F+         
Sbjct: 372 GQGMIYAMDVNEGRLRILGETAKSHQVDGLITTIH---------SDLRVFA--------- 431

Query: 284 PRSTQLTFDRVLCDVPCSGDGTLRKAPDIWRKWNSGMGNGLH--GLQVQIGMRGASLLKV 343
             + ++ +D+VL D PCSG G L K  D+  +WN  + + L    LQ ++    + L+K 
Sbjct: 432 -ETNEVQYDKVLLDAPCSGLGVLSKRADL--RWNRKLEDMLELTKLQDELLDSASKLVKH 478

Query: 344 GGRMVYSTCSMNPVENEA-VVAELLR 359
           GG +VYSTCS++P ENE  V A LLR
Sbjct: 492 GGVLVYSTCSIDPEENEGRVEAFLLR 478