Carg15965 (gene) Silver-seed gourd (SMH-JMG-627) v2

Overview
NameCarg15965
Typegene
OrganismCucurbita argyrosperma subsp. argyrosperma cv. SMH-JMG-627 (Silver-seed gourd (SMH-JMG-627) v2)
DescriptionMitochondrial transcription termination factor family protein
LocationCarg_Chr08: 7408822 .. 7409982 (+)
RNA-Seq ExpressionCarg15965
SyntenyCarg15965
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGTCTAATTTCCTCTGCAAAACCCTACTTCAGGTTCGGCATCTCACCACGATTTCGCCATATCCCAAGCTCCGCTGTGTTCAATCACTCCACTTCAACTACAGCACTGTCGCTTCCGATTCACATTCTTTTACGGTTTCGTATCTCATAAACTCATGCTCGTTGTCTCCTGAATCTGCTTCATCTGCTTCAAAGCTCGTTAGCTTCACTTCTCCTGAAAAACCAGATTTGGTGATCTCTTTCTTCAAGAATCACGGCTTTTCTATTGCTAATATCTCGTTCATTATCTGGAAATACCCTCGGATTCTGCTATCTAATCCGAATGGAACTCTTTTGCCTAAGCTTGAGTTTCTTTACACCAGAGGTGCTTCGAGTTCCGAAGTCACGAAGATTATTTCCAAGGCTCCGAAAATCTTGATGAGAAGCGTTGATGAACATCTGGTTCCTGCTTTTGATTTTTTGAAAGGTTATCTTGGATCTAATGAGCGAACCATCGCATCAATCAAACGCTTTCCGAGTATCATTGCGGATGTTCACCAAAAACAATTGAACGGATCTGTTAGAATGTTGCTCGACGCCGGCGTGCCTGAGGCGCGTGTTATGTTTTTTCTTTTTTACCAGCCGAGAATGTTCACTATGACTCTAGACAGGTTTAGGGAGATTTTGGAAGATGTGAAGAAAATGGGGTTTGATCCATCGAAATCTAGCTTTGTTCAAGCTGTTTTTGCTATACGAGCAATGAGCAAATCGACATGGGTACGGAAGCTTGGTATTTACAAGAGGTGGGGTTTATCTGAAGAACAGATTCTTGCTGCATTTAGGAGCCATCCATGGTGTATGACATTATCAGAGGAAAAAATTATGTTGGCAATGGATTTCTTTGTCAACAAATTAGGCTGGGAAACTGCAGCGATTGTCAGGAGTCCTTTGCTGCTTTCATTTAGCTTAAAAAAGAGAACAATTCCAAGAGCTTCAGTACTGCTGTTTCTGTTTCAGAAAGGTTTGACTAAGAAAAAAACCATGTTGTTGGTAAAACCATATATGTACACTGAAACTCAGTTCTTGCAGAAGTTTGTTCATCCTCATCTGAAAGAGGCTCCTCAGCTATCGAAGCTGTATCGTGAAAATGTGGATGGTGGGATAATCAATTATGAAATTTGA

mRNA sequence

ATGTCTAATTTCCTCTGCAAAACCCTACTTCAGGTTCGGCATCTCACCACGATTTCGCCATATCCCAAGCTCCGCTGTGTTCAATCACTCCACTTCAACTACAGCACTGTCGCTTCCGATTCACATTCTTTTACGGTTTCGTATCTCATAAACTCATGCTCGTTGTCTCCTGAATCTGCTTCATCTGCTTCAAAGCTCGTTAGCTTCACTTCTCCTGAAAAACCAGATTTGGTGATCTCTTTCTTCAAGAATCACGGCTTTTCTATTGCTAATATCTCGTTCATTATCTGGAAATACCCTCGGATTCTGCTATCTAATCCGAATGGAACTCTTTTGCCTAAGCTTGAGTTTCTTTACACCAGAGGTGCTTCGAGTTCCGAAGTCACGAAGATTATTTCCAAGGCTCCGAAAATCTTGATGAGAAGCGTTGATGAACATCTGGTTCCTGCTTTTGATTTTTTGAAAGGTTATCTTGGATCTAATGAGCGAACCATCGCATCAATCAAACGCTTTCCGAGTATCATTGCGGATGTTCACCAAAAACAATTGAACGGATCTGTTAGAATGTTGCTCGACGCCGGCGTGCCTGAGGCGCGTGTTATGTTTTTTCTTTTTTACCAGCCGAGAATGTTCACTATGACTCTAGACAGGTTTAGGGAGATTTTGGAAGATGTGAAGAAAATGGGGTTTGATCCATCGAAATCTAGCTTTGTTCAAGCTGTTTTTGCTATACGAGCAATGAGCAAATCGACATGGGTACGGAAGCTTGGTATTTACAAGAGGTGGGGTTTATCTGAAGAACAGATTCTTGCTGCATTTAGGAGCCATCCATGGTGTATGACATTATCAGAGGAAAAAATTATGTTGGCAATGGATTTCTTTGTCAACAAATTAGGCTGGGAAACTGCAGCGATTGTCAGGAGTCCTTTGCTGCTTTCATTTAGCTTAAAAAAGAGAACAATTCCAAGAGCTTCAGTACTGCTGTTTCTGTTTCAGAAAGGTTTGACTAAGAAAAAAACCATGTTGTTGGTAAAACCATATATGTACACTGAAACTCAGTTCTTGCAGAAGTTTGTTCATCCTCATCTGAAAGAGGCTCCTCAGCTATCGAAGCTGTATCGTGAAAATGTGGATGGTGGGATAATCAATTATGAAATTTGA

Coding sequence (CDS)

ATGTCTAATTTCCTCTGCAAAACCCTACTTCAGGTTCGGCATCTCACCACGATTTCGCCATATCCCAAGCTCCGCTGTGTTCAATCACTCCACTTCAACTACAGCACTGTCGCTTCCGATTCACATTCTTTTACGGTTTCGTATCTCATAAACTCATGCTCGTTGTCTCCTGAATCTGCTTCATCTGCTTCAAAGCTCGTTAGCTTCACTTCTCCTGAAAAACCAGATTTGGTGATCTCTTTCTTCAAGAATCACGGCTTTTCTATTGCTAATATCTCGTTCATTATCTGGAAATACCCTCGGATTCTGCTATCTAATCCGAATGGAACTCTTTTGCCTAAGCTTGAGTTTCTTTACACCAGAGGTGCTTCGAGTTCCGAAGTCACGAAGATTATTTCCAAGGCTCCGAAAATCTTGATGAGAAGCGTTGATGAACATCTGGTTCCTGCTTTTGATTTTTTGAAAGGTTATCTTGGATCTAATGAGCGAACCATCGCATCAATCAAACGCTTTCCGAGTATCATTGCGGATGTTCACCAAAAACAATTGAACGGATCTGTTAGAATGTTGCTCGACGCCGGCGTGCCTGAGGCGCGTGTTATGTTTTTTCTTTTTTACCAGCCGAGAATGTTCACTATGACTCTAGACAGGTTTAGGGAGATTTTGGAAGATGTGAAGAAAATGGGGTTTGATCCATCGAAATCTAGCTTTGTTCAAGCTGTTTTTGCTATACGAGCAATGAGCAAATCGACATGGGTACGGAAGCTTGGTATTTACAAGAGGTGGGGTTTATCTGAAGAACAGATTCTTGCTGCATTTAGGAGCCATCCATGGTGTATGACATTATCAGAGGAAAAAATTATGTTGGCAATGGATTTCTTTGTCAACAAATTAGGCTGGGAAACTGCAGCGATTGTCAGGAGTCCTTTGCTGCTTTCATTTAGCTTAAAAAAGAGAACAATTCCAAGAGCTTCAGTACTGCTGTTTCTGTTTCAGAAAGGTTTGACTAAGAAAAAAACCATGTTGTTGGTAAAACCATATATGTACACTGAAACTCAGTTCTTGCAGAAGTTTGTTCATCCTCATCTGAAAGAGGCTCCTCAGCTATCGAAGCTGTATCGTGAAAATGTGGATGGTGGGATAATCAATTATGAAATTTGA

Protein sequence

MSNFLCKTLLQVRHLTTISPYPKLRCVQSLHFNYSTVASDSHSFTVSYLINSCSLSPESASSASKLVSFTSPEKPDLVISFFKNHGFSIANISFIIWKYPRILLSNPNGTLLPKLEFLYTRGASSSEVTKIISKAPKILMRSVDEHLVPAFDFLKGYLGSNERTIASIKRFPSIIADVHQKQLNGSVRMLLDAGVPEARVMFFLFYQPRMFTMTLDRFREILEDVKKMGFDPSKSSFVQAVFAIRAMSKSTWVRKLGIYKRWGLSEEQILAAFRSHPWCMTLSEEKIMLAMDFFVNKLGWETAAIVRSPLLLSFSLKKRTIPRASVLLFLFQKGLTKKKTMLLVKPYMYTETQFLQKFVHPHLKEAPQLSKLYRENVDGGIINYEI
Homology
BLAST of Carg15965 vs. NCBI nr
Match: KAG7026295.1 (Transcription termination factor MTERF8, chloroplastic, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 757.7 bits (1955), Expect = 5.0e-215
Identity = 386/386 (100.00%), Postives = 386/386 (100.00%), Query Frame = 0

Query: 1   MSNFLCKTLLQVRHLTTISPYPKLRCVQSLHFNYSTVASDSHSFTVSYLINSCSLSPESA 60
           MSNFLCKTLLQVRHLTTISPYPKLRCVQSLHFNYSTVASDSHSFTVSYLINSCSLSPESA
Sbjct: 1   MSNFLCKTLLQVRHLTTISPYPKLRCVQSLHFNYSTVASDSHSFTVSYLINSCSLSPESA 60

Query: 61  SSASKLVSFTSPEKPDLVISFFKNHGFSIANISFIIWKYPRILLSNPNGTLLPKLEFLYT 120
           SSASKLVSFTSPEKPDLVISFFKNHGFSIANISFIIWKYPRILLSNPNGTLLPKLEFLYT
Sbjct: 61  SSASKLVSFTSPEKPDLVISFFKNHGFSIANISFIIWKYPRILLSNPNGTLLPKLEFLYT 120

Query: 121 RGASSSEVTKIISKAPKILMRSVDEHLVPAFDFLKGYLGSNERTIASIKRFPSIIADVHQ 180
           RGASSSEVTKIISKAPKILMRSVDEHLVPAFDFLKGYLGSNERTIASIKRFPSIIADVHQ
Sbjct: 121 RGASSSEVTKIISKAPKILMRSVDEHLVPAFDFLKGYLGSNERTIASIKRFPSIIADVHQ 180

Query: 181 KQLNGSVRMLLDAGVPEARVMFFLFYQPRMFTMTLDRFREILEDVKKMGFDPSKSSFVQA 240
           KQLNGSVRMLLDAGVPEARVMFFLFYQPRMFTMTLDRFREILEDVKKMGFDPSKSSFVQA
Sbjct: 181 KQLNGSVRMLLDAGVPEARVMFFLFYQPRMFTMTLDRFREILEDVKKMGFDPSKSSFVQA 240

Query: 241 VFAIRAMSKSTWVRKLGIYKRWGLSEEQILAAFRSHPWCMTLSEEKIMLAMDFFVNKLGW 300
           VFAIRAMSKSTWVRKLGIYKRWGLSEEQILAAFRSHPWCMTLSEEKIMLAMDFFVNKLGW
Sbjct: 241 VFAIRAMSKSTWVRKLGIYKRWGLSEEQILAAFRSHPWCMTLSEEKIMLAMDFFVNKLGW 300

Query: 301 ETAAIVRSPLLLSFSLKKRTIPRASVLLFLFQKGLTKKKTMLLVKPYMYTETQFLQKFVH 360
           ETAAIVRSPLLLSFSLKKRTIPRASVLLFLFQKGLTKKKTMLLVKPYMYTETQFLQKFVH
Sbjct: 301 ETAAIVRSPLLLSFSLKKRTIPRASVLLFLFQKGLTKKKTMLLVKPYMYTETQFLQKFVH 360

Query: 361 PHLKEAPQLSKLYRENVDGGIINYEI 387
           PHLKEAPQLSKLYRENVDGGIINYEI
Sbjct: 361 PHLKEAPQLSKLYRENVDGGIINYEI 386

BLAST of Carg15965 vs. NCBI nr
Match: KAG6593956.1 (Transcription termination factor MTERF8, chloroplastic, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 748.4 bits (1931), Expect = 3.0e-212
Identity = 383/386 (99.22%), Postives = 384/386 (99.48%), Query Frame = 0

Query: 1   MSNFLCKTLLQVRHLTTISPYPKLRCVQSLHFNYSTVASDSHSFTVSYLINSCSLSPESA 60
           MSNFLC+TLLQVRHLTTISPYPKLRCVQSLHFNYSTVASDSHSFTVSYLINSCSLSPESA
Sbjct: 1   MSNFLCRTLLQVRHLTTISPYPKLRCVQSLHFNYSTVASDSHSFTVSYLINSCSLSPESA 60

Query: 61  SSASKLVSFTSPEKPDLVISFFKNHGFSIANISFIIWKYPRILLSNPNGTLLPKLEFLYT 120
           SSASKLVSFTSPEKPDLVISFFKNHGFSIANISFIIWKYPRILLSNPNGTLLPKLEFLYT
Sbjct: 61  SSASKLVSFTSPEKPDLVISFFKNHGFSIANISFIIWKYPRILLSNPNGTLLPKLEFLYT 120

Query: 121 RGASSSEVTKIISKAPKILMRSVDEHLVPAFDFLKGYLGSNERTIASIKRFPSIIADVHQ 180
           RGASSSEVTKIISKAPKILMRSVDEHLVPAFDFLKGYLGSNERTIASIKRFPSIIADVHQ
Sbjct: 121 RGASSSEVTKIISKAPKILMRSVDEHLVPAFDFLKGYLGSNERTIASIKRFPSIIADVHQ 180

Query: 181 KQLNGSVRMLLDAGVPEARVMFFLFYQPRMFTMTLDRFREILEDVKKMGFDPSKSSFVQA 240
           K LNGSVRMLLDAGVPEARVMFFLFYQPRMFTMTLDRFREILEDVKKMGFDPSKSSFVQA
Sbjct: 181 KHLNGSVRMLLDAGVPEARVMFFLFYQPRMFTMTLDRFREILEDVKKMGFDPSKSSFVQA 240

Query: 241 VFAIRAMSKSTWVRKLGIYKRWGLSEEQILAAFRSHPWCMTLSEEKIMLAMDFFVNKLGW 300
           VFAIRAMSKSTWVRKLGIYKRWGLSEEQILAAFRSHPWCMTLSEEKIMLAMDFFVNKLGW
Sbjct: 241 VFAIRAMSKSTWVRKLGIYKRWGLSEEQILAAFRSHPWCMTLSEEKIMLAMDFFVNKLGW 300

Query: 301 ETAAIVRSPLLLSFSLKKRTIPRASVLLFLFQKGLTKKKTMLLVKPYMYTETQFLQKFVH 360
           ETAAIVRSPLLLSFSLKKRTIPRASVLLFLFQKGLTKKKTM LVKPYMYTETQFLQKFVH
Sbjct: 301 ETAAIVRSPLLLSFSLKKRTIPRASVLLFLFQKGLTKKKTM-LVKPYMYTETQFLQKFVH 360

Query: 361 PHLKEAPQLSKLYRENVDGGIINYEI 387
           PHLKEAPQLSKLYRENVDGGIINYEI
Sbjct: 361 PHLKEAPQLSKLYRENVDGGIINYEI 385

BLAST of Carg15965 vs. NCBI nr
Match: XP_022930344.1 (transcription termination factor MTERF6, chloroplastic/mitochondrial-like [Cucurbita moschata])

HSP 1 Score: 745.7 bits (1924), Expect = 2.0e-211
Identity = 381/386 (98.70%), Postives = 384/386 (99.48%), Query Frame = 0

Query: 1   MSNFLCKTLLQVRHLTTISPYPKLRCVQSLHFNYSTVASDSHSFTVSYLINSCSLSPESA 60
           MSNFLCKTLLQVRHLTTISPYPKLRCVQSLHFNYSTVASDSHSFTVSYLINSCSLSPESA
Sbjct: 1   MSNFLCKTLLQVRHLTTISPYPKLRCVQSLHFNYSTVASDSHSFTVSYLINSCSLSPESA 60

Query: 61  SSASKLVSFTSPEKPDLVISFFKNHGFSIANISFIIWKYPRILLSNPNGTLLPKLEFLYT 120
           SSASKLVSFTSPEKPDLVISFFKNHGFSIANISFIIWKYPRILLSNPNGTLLPKLEFLYT
Sbjct: 61  SSASKLVSFTSPEKPDLVISFFKNHGFSIANISFIIWKYPRILLSNPNGTLLPKLEFLYT 120

Query: 121 RGASSSEVTKIISKAPKILMRSVDEHLVPAFDFLKGYLGSNERTIASIKRFPSIIADVHQ 180
           RGASSSEVTKIISKAPKILMRSVDEHLVPAFDFLKGYLGSNERTIASIKRFPSIIAD+HQ
Sbjct: 121 RGASSSEVTKIISKAPKILMRSVDEHLVPAFDFLKGYLGSNERTIASIKRFPSIIADIHQ 180

Query: 181 KQLNGSVRMLLDAGVPEARVMFFLFYQPRMFTMTLDRFREILEDVKKMGFDPSKSSFVQA 240
           KQLNGSVRMLLDAGVPEARV+FFLFYQPRMFTM LDRFREILEDVKKMGFDPSKSSFVQA
Sbjct: 181 KQLNGSVRMLLDAGVPEARVVFFLFYQPRMFTMALDRFREILEDVKKMGFDPSKSSFVQA 240

Query: 241 VFAIRAMSKSTWVRKLGIYKRWGLSEEQILAAFRSHPWCMTLSEEKIMLAMDFFVNKLGW 300
           VFAIRAMSKSTWVRKLGIYKRWGLSEEQILAAFRSHPWCMTLSEEKIMLAMDFFVNKLGW
Sbjct: 241 VFAIRAMSKSTWVRKLGIYKRWGLSEEQILAAFRSHPWCMTLSEEKIMLAMDFFVNKLGW 300

Query: 301 ETAAIVRSPLLLSFSLKKRTIPRASVLLFLFQKGLTKKKTMLLVKPYMYTETQFLQKFVH 360
           ETAAIVRSPLLLSFSLKKRTIPRASVLLFLFQKGLTKKKTM LVKPYMYTETQFLQKFVH
Sbjct: 301 ETAAIVRSPLLLSFSLKKRTIPRASVLLFLFQKGLTKKKTM-LVKPYMYTETQFLQKFVH 360

Query: 361 PHLKEAPQLSKLYRENVDGGIINYEI 387
           PHLKEAPQLSKLYRENVDGGII+YEI
Sbjct: 361 PHLKEAPQLSKLYRENVDGGIIDYEI 385

BLAST of Carg15965 vs. NCBI nr
Match: XP_023514950.1 (transcription termination factor MTERF6, chloroplastic/mitochondrial-like isoform X1 [Cucurbita pepo subsp. pepo] >XP_023514951.1 transcription termination factor MTERF6, chloroplastic/mitochondrial-like isoform X2 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 723.0 bits (1865), Expect = 1.4e-204
Identity = 371/386 (96.11%), Postives = 376/386 (97.41%), Query Frame = 0

Query: 1   MSNFLCKTLLQVRHLTTISPYPKLRCVQSLHFNYSTVASDSHSFTVSYLINSCSLSPESA 60
           MS FLCKTLLQVRHLTTISPY KLRCVQSLHFNYSTVASDSHSFTVSYLINSCSLSPESA
Sbjct: 1   MSKFLCKTLLQVRHLTTISPYSKLRCVQSLHFNYSTVASDSHSFTVSYLINSCSLSPESA 60

Query: 61  SSASKLVSFTSPEKPDLVISFFKNHGFSIANISFIIWKYPRILLSNPNGTLLPKLEFLYT 120
           SSASKLVSF SPEKPDLVISFFKNHGFSIANISFIIWKYPR+LLSNPNGTLLPKLEFLYT
Sbjct: 61  SSASKLVSFISPEKPDLVISFFKNHGFSIANISFIIWKYPRVLLSNPNGTLLPKLEFLYT 120

Query: 121 RGASSSEVTKIISKAPKILMRSVDEHLVPAFDFLKGYLGSNERTIASIKRFPSIIADVHQ 180
           RGASSSEVTKIISKAPKILMRSVDEHLVPAFDFLKGYLGSNERTIASIKRFPSIIAD+HQ
Sbjct: 121 RGASSSEVTKIISKAPKILMRSVDEHLVPAFDFLKGYLGSNERTIASIKRFPSIIADIHQ 180

Query: 181 KQLNGSVRMLLDAGVPEARVMFFLFYQPRMFTMTLDRFREILEDVKKMGFDPSKSSFVQA 240
           KQL+GSVRMLLDAGVPEARVMFFLFYQPRMFTM LDRFREILEDVKKMGFDPSKSSFVQA
Sbjct: 181 KQLSGSVRMLLDAGVPEARVMFFLFYQPRMFTMALDRFREILEDVKKMGFDPSKSSFVQA 240

Query: 241 VFAIRAMSKSTWVRKLGIYKRWGLSEEQILAAFRSHPWCMTLSEEKIMLAMDFFVNKLGW 300
           VFAIRAMSKSTWVRKLGIYKRWGLSEEQILAAFRSHPWCMTLSEEKIMLAMDFFVNKLGW
Sbjct: 241 VFAIRAMSKSTWVRKLGIYKRWGLSEEQILAAFRSHPWCMTLSEEKIMLAMDFFVNKLGW 300

Query: 301 ETAAIVRSPLLLSFSLKKRTIPRASVLLFLFQKGLTKKKTMLLVKPYMYTETQFLQKFVH 360
           ETAAIVRSPLLLS SLKKR IPRASVLLFLFQKGLT KKTM LVKPYMYTETQFL+KFV 
Sbjct: 301 ETAAIVRSPLLLSLSLKKRIIPRASVLLFLFQKGLTTKKTM-LVKPYMYTETQFLEKFVR 360

Query: 361 PHLKEAPQLSKLYRENVDGGIINYEI 387
           PHLKEAPQLSKLYRENVD GII+YEI
Sbjct: 361 PHLKEAPQLSKLYRENVDRGIIDYEI 385

BLAST of Carg15965 vs. NCBI nr
Match: XP_022930343.1 (transcription termination factor MTERF6, chloroplastic/mitochondrial-like [Cucurbita moschata])

HSP 1 Score: 699.9 bits (1805), Expect = 1.2e-197
Identity = 359/386 (93.01%), Postives = 369/386 (95.60%), Query Frame = 0

Query: 1   MSNFLCKTLLQVRHLTTISPYPKLRCVQSLHFNYSTVASDSHSFTVSYLINSCSLSPESA 60
           MSNFLCKTLLQVRHLTTISPYPKLRCVQSLHFNYSTVASDSHSFTVSYLINSCSLSPESA
Sbjct: 1   MSNFLCKTLLQVRHLTTISPYPKLRCVQSLHFNYSTVASDSHSFTVSYLINSCSLSPESA 60

Query: 61  SSASKLVSFTSPEKPDLVISFFKNHGFSIANISFIIWKYPRILLSNPNGTLLPKLEFLYT 120
           SSASKLVSFTSPEKPDLVISFFKNHGFS A+IS II KYPR+LLSNPNGTLLPKLEFLYT
Sbjct: 61  SSASKLVSFTSPEKPDLVISFFKNHGFSNAHISLIIGKYPRMLLSNPNGTLLPKLEFLYT 120

Query: 121 RGASSSEVTKIISKAPKILMRSVDEHLVPAFDFLKGYLGSNERTIASIKRFPSIIADVHQ 180
           RGASSSEV  IIS+AP IL RSVDEHLVPAFD LKGYLGSNERTIASIKRFPS+I D+ Q
Sbjct: 121 RGASSSEVATIISRAPNILTRSVDEHLVPAFDLLKGYLGSNERTIASIKRFPSVIGDIRQ 180

Query: 181 KQLNGSVRMLLDAGVPEARVMFFLFYQPRMFTMTLDRFREILEDVKKMGFDPSKSSFVQA 240
           K L+GSV MLLDAGVPEA VM+FLFYQPRMFTM LDRFREILEDVKKMGFDPSKSSFVQA
Sbjct: 181 KYLSGSVTMLLDAGVPEALVMYFLFYQPRMFTMALDRFREILEDVKKMGFDPSKSSFVQA 240

Query: 241 VFAIRAMSKSTWVRKLGIYKRWGLSEEQILAAFRSHPWCMTLSEEKIMLAMDFFVNKLGW 300
           V A+R MSKSTWVRKLGI+KRWGLSEEQILAAFRSHPWCMTLSEEKIMLAMDFFVNKLGW
Sbjct: 241 VHAMRGMSKSTWVRKLGIFKRWGLSEEQILAAFRSHPWCMTLSEEKIMLAMDFFVNKLGW 300

Query: 301 ETAAIVRSPLLLSFSLKKRTIPRASVLLFLFQKGLTKKKTMLLVKPYMYTETQFLQKFVH 360
           ETAAIVRSPLLLSFSLKKRTIPRASVLLFLFQKGLTKKKTM LVKPYMYTETQFLQKFVH
Sbjct: 301 ETAAIVRSPLLLSFSLKKRTIPRASVLLFLFQKGLTKKKTM-LVKPYMYTETQFLQKFVH 360

Query: 361 PHLKEAPQLSKLYRENVDGGIINYEI 387
           PHLKEAPQLSKLYRENVDGGII+YEI
Sbjct: 361 PHLKEAPQLSKLYRENVDGGIIDYEI 385

BLAST of Carg15965 vs. ExPASy Swiss-Prot
Match: Q9FK23 (Transcription termination factor MTERF8, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=MTERF8 PE=1 SV=1)

HSP 1 Score: 84.0 bits (206), Expect = 4.2e-15
Identity = 64/258 (24.81%), Postives = 119/258 (46.12%), Query Frame = 0

Query: 64  SKLVSFTSPEKPDLVISFFKNHGFSIANISFIIWKYPRILLSNPNGTLLPKLEFLYTRGA 123
           +KL+   S E  + +ISF +  G     I  II + P IL S+ +  L+P+++F+     
Sbjct: 208 NKLLYQKSVEDIERLISFLEPFG----GIG-IIARRPVILNSDLDSQLIPRVDFIRNLSG 267

Query: 124 SSSEVT-KIISKAPKILMRSVDEHLVPAFDFLKGYLG-SNERTIASIKRFPSIIADVHQK 183
                T  ++ + P IL  SV EH+    +FLK + G ++E+    +  FP++I+   ++
Sbjct: 268 EDDFATGTVLRRLPAILSYSV-EHMNGQVEFLKSFAGLTSEQVFKIVHVFPNVISTSKER 327

Query: 184 QLNGSVRMLLDAGVPEARVMFFLFYQPRMFTMTLDRFREILEDVKKMGFDPSKSSFVQAV 243
           +L   +  L + G     +  FL   P +  ++ +     L  + K+G+         A+
Sbjct: 328 KLRPRIEFLKECGFDSPGMFKFLSKAPLILALSENNLSHKLGFLVKIGYKHRTKELAFAM 387

Query: 244 FAIRAMSKSTWVRKLGIYKRWGLSEEQILAAFRSHPWCMTLSEEKIMLAMDFFVNKLGWE 303
            A+   S     R +G+Y  +GLS E ILA    HP  +  +   +   +++ +  +G E
Sbjct: 388 GAVTRTSSDNMQRVIGLYLSYGLSFEDILAMSTKHPQVLQYNYTSLEEKLEYLIEYMGRE 447

Query: 304 TAAIVRSPLLLSFSLKKR 320
              ++  P  L + L  R
Sbjct: 448 VEELLAFPAFLGYKLDSR 459

BLAST of Carg15965 vs. ExPASy Swiss-Prot
Match: Q9SZL6 (Transcription termination factor MTERF6, chloroplastic/mitochondrial OS=Arabidopsis thaliana OX=3702 GN=MTERF6 PE=2 SV=1)

HSP 1 Score: 64.3 bits (155), Expect = 3.4e-09
Identity = 63/277 (22.74%), Postives = 125/277 (45.13%), Query Frame = 0

Query: 78  VISFFKNHGFSIANISFIIWKYPRIL-----LSNPNGTLLPKLEFLYTRGASSSEVTKII 137
           ++ FF++ GF   +I  ++ K  ++      +++ N   L  +      G    ++  I+
Sbjct: 9   IMWFFRDKGFDDPSIDKMLRKCKQLEKAQSDVASENWDYLSNIV-----GIQERKLPYIV 68

Query: 138 SKAPKILMRSVDEHLVPAFDFLKGYLGSNERTIAS-IKRFPSIIADVHQKQLNGSVRMLL 197
           S+ PKIL   +DE L+P  + L   LG N R +AS I +FP I++   +++L   +    
Sbjct: 69  SRCPKILTLRLDERLIPMVECLSS-LGRNPREVASAITKFPPILSHSVEEKLCPLLAFFQ 128

Query: 198 DAGVPEARVMFFLFYQPRMFTMTLD-RFREILEDVKKMGFDPS--------KSSFVQAVF 257
             GVPE ++   + + PR+ + ++D +   I+  +  +G D          K+ F+    
Sbjct: 129 ALGVPETQLGKMILFNPRLISYSIDTKLTVIVSFLASLGLDQDGMIGKVLVKNPFLMGYS 188

Query: 258 AIRAMSKSTWVRKLGIYKRWGLSEEQILAAFRSHPWCMTLSEEKIMLAMDFFVNKLGWET 317
             + +  +T   K  +    GLSE+ I +   + P  +     KI+     ++ + G+  
Sbjct: 189 VDKRLRPTTEFLKSSV----GLSEDGIKSVVMNFPQLLCRDVNKILKPNYDYLKECGFGD 248

Query: 318 AAIVRS----PLLLSFSLKKRTIPRASVLLFLFQKGL 336
           + I       P +L  S+K    PR   L+ +  +G+
Sbjct: 249 SQIATMVTGYPQILIKSVKNSLQPRIRFLVQVMGRGM 275

BLAST of Carg15965 vs. ExPASy Swiss-Prot
Match: Q84X53 (Transcription termination factor MTEF1, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=MTERF1 PE=2 SV=2)

HSP 1 Score: 52.8 bits (125), Expect = 1.0e-05
Identity = 46/172 (26.74%), Postives = 78/172 (45.35%), Query Frame = 0

Query: 78  VISFFKNHGFSIANISFIIWKYPRILLSNPNGTLLPKLEFLYTR-GASSSEVTKIISKAP 137
           V +   + G S   +  I+  +P +L S+P   +LP L FL      S  ++ K IS+ P
Sbjct: 68  VETLLSSTGLSRPAVGRILDMFPDLLTSDPESEILPVLRFLSNEISISEQDIPKSISRCP 127

Query: 138 KILMRSVDEHLVPAFDFLK--GYLGSNERTIASIKRFPSIIADVHQKQLNGSVRMLLDAG 197
           ++L+ SVD  L PA  FLK  G++G +  TI S +    ++++V +  +     +    G
Sbjct: 128 RLLISSVDYQLRPALTFLKTLGFVGRD--TITS-RNTVLLVSNVERTLIPKIEYLEEGLG 187

Query: 198 VPEARVMFFLFYQPRMFTMTLDR---------FREILEDVKKMGFDPSKSSF 238
                V   +   P + T ++D            E+  DVK++   P   SF
Sbjct: 188 FTREEVAKMVVRSPALLTYSVDNNLVPKVEFFIEEMRGDVKELKRFPQYFSF 236

BLAST of Carg15965 vs. ExPASy TrEMBL
Match: A0A6J1ER69 (transcription termination factor MTERF6, chloroplastic/mitochondrial-like OS=Cucurbita moschata OX=3662 GN=LOC111436817 PE=3 SV=1)

HSP 1 Score: 745.7 bits (1924), Expect = 9.5e-212
Identity = 381/386 (98.70%), Postives = 384/386 (99.48%), Query Frame = 0

Query: 1   MSNFLCKTLLQVRHLTTISPYPKLRCVQSLHFNYSTVASDSHSFTVSYLINSCSLSPESA 60
           MSNFLCKTLLQVRHLTTISPYPKLRCVQSLHFNYSTVASDSHSFTVSYLINSCSLSPESA
Sbjct: 1   MSNFLCKTLLQVRHLTTISPYPKLRCVQSLHFNYSTVASDSHSFTVSYLINSCSLSPESA 60

Query: 61  SSASKLVSFTSPEKPDLVISFFKNHGFSIANISFIIWKYPRILLSNPNGTLLPKLEFLYT 120
           SSASKLVSFTSPEKPDLVISFFKNHGFSIANISFIIWKYPRILLSNPNGTLLPKLEFLYT
Sbjct: 61  SSASKLVSFTSPEKPDLVISFFKNHGFSIANISFIIWKYPRILLSNPNGTLLPKLEFLYT 120

Query: 121 RGASSSEVTKIISKAPKILMRSVDEHLVPAFDFLKGYLGSNERTIASIKRFPSIIADVHQ 180
           RGASSSEVTKIISKAPKILMRSVDEHLVPAFDFLKGYLGSNERTIASIKRFPSIIAD+HQ
Sbjct: 121 RGASSSEVTKIISKAPKILMRSVDEHLVPAFDFLKGYLGSNERTIASIKRFPSIIADIHQ 180

Query: 181 KQLNGSVRMLLDAGVPEARVMFFLFYQPRMFTMTLDRFREILEDVKKMGFDPSKSSFVQA 240
           KQLNGSVRMLLDAGVPEARV+FFLFYQPRMFTM LDRFREILEDVKKMGFDPSKSSFVQA
Sbjct: 181 KQLNGSVRMLLDAGVPEARVVFFLFYQPRMFTMALDRFREILEDVKKMGFDPSKSSFVQA 240

Query: 241 VFAIRAMSKSTWVRKLGIYKRWGLSEEQILAAFRSHPWCMTLSEEKIMLAMDFFVNKLGW 300
           VFAIRAMSKSTWVRKLGIYKRWGLSEEQILAAFRSHPWCMTLSEEKIMLAMDFFVNKLGW
Sbjct: 241 VFAIRAMSKSTWVRKLGIYKRWGLSEEQILAAFRSHPWCMTLSEEKIMLAMDFFVNKLGW 300

Query: 301 ETAAIVRSPLLLSFSLKKRTIPRASVLLFLFQKGLTKKKTMLLVKPYMYTETQFLQKFVH 360
           ETAAIVRSPLLLSFSLKKRTIPRASVLLFLFQKGLTKKKTM LVKPYMYTETQFLQKFVH
Sbjct: 301 ETAAIVRSPLLLSFSLKKRTIPRASVLLFLFQKGLTKKKTM-LVKPYMYTETQFLQKFVH 360

Query: 361 PHLKEAPQLSKLYRENVDGGIINYEI 387
           PHLKEAPQLSKLYRENVDGGII+YEI
Sbjct: 361 PHLKEAPQLSKLYRENVDGGIIDYEI 385

BLAST of Carg15965 vs. ExPASy TrEMBL
Match: A0A6J1EUW1 (transcription termination factor MTERF6, chloroplastic/mitochondrial-like OS=Cucurbita moschata OX=3662 GN=LOC111436816 PE=3 SV=1)

HSP 1 Score: 699.9 bits (1805), Expect = 5.9e-198
Identity = 359/386 (93.01%), Postives = 369/386 (95.60%), Query Frame = 0

Query: 1   MSNFLCKTLLQVRHLTTISPYPKLRCVQSLHFNYSTVASDSHSFTVSYLINSCSLSPESA 60
           MSNFLCKTLLQVRHLTTISPYPKLRCVQSLHFNYSTVASDSHSFTVSYLINSCSLSPESA
Sbjct: 1   MSNFLCKTLLQVRHLTTISPYPKLRCVQSLHFNYSTVASDSHSFTVSYLINSCSLSPESA 60

Query: 61  SSASKLVSFTSPEKPDLVISFFKNHGFSIANISFIIWKYPRILLSNPNGTLLPKLEFLYT 120
           SSASKLVSFTSPEKPDLVISFFKNHGFS A+IS II KYPR+LLSNPNGTLLPKLEFLYT
Sbjct: 61  SSASKLVSFTSPEKPDLVISFFKNHGFSNAHISLIIGKYPRMLLSNPNGTLLPKLEFLYT 120

Query: 121 RGASSSEVTKIISKAPKILMRSVDEHLVPAFDFLKGYLGSNERTIASIKRFPSIIADVHQ 180
           RGASSSEV  IIS+AP IL RSVDEHLVPAFD LKGYLGSNERTIASIKRFPS+I D+ Q
Sbjct: 121 RGASSSEVATIISRAPNILTRSVDEHLVPAFDLLKGYLGSNERTIASIKRFPSVIGDIRQ 180

Query: 181 KQLNGSVRMLLDAGVPEARVMFFLFYQPRMFTMTLDRFREILEDVKKMGFDPSKSSFVQA 240
           K L+GSV MLLDAGVPEA VM+FLFYQPRMFTM LDRFREILEDVKKMGFDPSKSSFVQA
Sbjct: 181 KYLSGSVTMLLDAGVPEALVMYFLFYQPRMFTMALDRFREILEDVKKMGFDPSKSSFVQA 240

Query: 241 VFAIRAMSKSTWVRKLGIYKRWGLSEEQILAAFRSHPWCMTLSEEKIMLAMDFFVNKLGW 300
           V A+R MSKSTWVRKLGI+KRWGLSEEQILAAFRSHPWCMTLSEEKIMLAMDFFVNKLGW
Sbjct: 241 VHAMRGMSKSTWVRKLGIFKRWGLSEEQILAAFRSHPWCMTLSEEKIMLAMDFFVNKLGW 300

Query: 301 ETAAIVRSPLLLSFSLKKRTIPRASVLLFLFQKGLTKKKTMLLVKPYMYTETQFLQKFVH 360
           ETAAIVRSPLLLSFSLKKRTIPRASVLLFLFQKGLTKKKTM LVKPYMYTETQFLQKFVH
Sbjct: 301 ETAAIVRSPLLLSFSLKKRTIPRASVLLFLFQKGLTKKKTM-LVKPYMYTETQFLQKFVH 360

Query: 361 PHLKEAPQLSKLYRENVDGGIINYEI 387
           PHLKEAPQLSKLYRENVDGGII+YEI
Sbjct: 361 PHLKEAPQLSKLYRENVDGGIIDYEI 385

BLAST of Carg15965 vs. ExPASy TrEMBL
Match: A0A6J1KHT4 (transcription termination factor MTERF6, chloroplastic/mitochondrial-like OS=Cucurbita maxima OX=3661 GN=LOC111494528 PE=3 SV=1)

HSP 1 Score: 695.7 bits (1794), Expect = 1.1e-196
Identity = 357/381 (93.70%), Postives = 366/381 (96.06%), Query Frame = 0

Query: 1   MSNFLCKTLLQVRHLTTISPYPKLRCVQSLHFNYSTVASDSHSFTVSYLINSCSLSPESA 60
           MSNFLCKTLLQVRHLTTISPYPKLRCVQSLHFNYSTV+SDSHSFTVSYLINSCSLSPESA
Sbjct: 1   MSNFLCKTLLQVRHLTTISPYPKLRCVQSLHFNYSTVSSDSHSFTVSYLINSCSLSPESA 60

Query: 61  SSASKLVSFTSPEKPDLVISFFKNHGFSIANISFIIWKYPRILLSNPNGTLLPKLEFLYT 120
           SSASKLVSF SPEKPDLVISFFKNHGFSIA+ISFII KYPRILLSNPNG LLP+LEFLYT
Sbjct: 61  SSASKLVSFISPEKPDLVISFFKNHGFSIAHISFIIGKYPRILLSNPNGALLPRLEFLYT 120

Query: 121 RGASSSEVTKIISKAPKILMRSVDEHLVPAFDFLKGYLGSNERTIASIKRFPSIIADVHQ 180
           RGASSSEVTKIISKAPKILMRSVDEHLVPAFD LKGYLGSNERTIASIKRFPSIIAD+ Q
Sbjct: 121 RGASSSEVTKIISKAPKILMRSVDEHLVPAFDLLKGYLGSNERTIASIKRFPSIIADIRQ 180

Query: 181 KQLNGSVRMLLDAGVPEARVMFFLFYQPRMFTMTLDRFREILEDVKKMGFDPSKSSFVQA 240
           K L+GSVRMLLDAGVPEA VM+FLFYQPRMFTM LDRFREILEDVKKMGFDPSKSSF+QA
Sbjct: 181 KHLSGSVRMLLDAGVPEAHVMYFLFYQPRMFTMALDRFREILEDVKKMGFDPSKSSFIQA 240

Query: 241 VFAIRAMSKSTWVRKLGIYKRWGLSEEQILAAFRSHPWCMTLSEEKIMLAMDFFVNKLGW 300
           V AIR MSKSTWVRKLGIYKRWGLSEE+ILAAFRSHPWCMTLSEEKIMLAMDFFV KLGW
Sbjct: 241 VHAIRGMSKSTWVRKLGIYKRWGLSEEEILAAFRSHPWCMTLSEEKIMLAMDFFVKKLGW 300

Query: 301 ETAAIVRSPLLLSFSLKKRTIPRASVLLFLFQKGLTKKKTMLLVKPYMYTETQFLQKFVH 360
           ETAAIVRSP+LLSFSLKKRTIPRASVLLFLFQKGLT KKTM LVKPYMYTETQFLQKFV 
Sbjct: 301 ETAAIVRSPVLLSFSLKKRTIPRASVLLFLFQKGLTTKKTM-LVKPYMYTETQFLQKFVR 360

Query: 361 PHLKEAPQLSKLYRENVDGGI 382
           PHLKEAPQLSKLYR NVDGGI
Sbjct: 361 PHLKEAPQLSKLYRINVDGGI 380

BLAST of Carg15965 vs. ExPASy TrEMBL
Match: A0A6J1CAW8 (transcription termination factor MTERF6, chloroplastic/mitochondrial-like OS=Momordica charantia OX=3673 GN=LOC111009559 PE=3 SV=1)

HSP 1 Score: 610.1 bits (1572), Expect = 6.2e-171
Identity = 315/382 (82.46%), Postives = 341/382 (89.27%), Query Frame = 0

Query: 1   MSNFLCKTLLQVRHLTTISPYPKLRCVQSLHFNYSTVA-SDSHSFTVSYLINSCSLSPES 60
           MSNFLCKTL++VRHL T SPYP LRCVQ LH +Y+T A SDSHSFTVSYL+NSCS SPES
Sbjct: 1   MSNFLCKTLIRVRHLLTNSPYPNLRCVQFLHVSYTTAAVSDSHSFTVSYLVNSCSFSPES 60

Query: 61  ASSASKLVSFTSPEKPDLVISFFKNHGFSIANISFIIWKYPRILLSNPNGTLLPKLEFLY 120
           A SASKLVSF + +KPD VISFFKN GFS  +IS II KYPR+LLS+PN TLLPKLEFLY
Sbjct: 61  ALSASKLVSFDARDKPDSVISFFKNRGFSNIHISLIIGKYPRLLLSDPNKTLLPKLEFLY 120

Query: 121 TRGASSSEVTKIISKAPKILMRSVDEHLVPAFDFLKGYLGSNERTIASIKRFPSIIADVH 180
           +RGASSSEV+KII KAPKIL RSV++HL+PAFDFLKGYL SNERTIASIKRFPSIIAD  
Sbjct: 121 SRGASSSEVSKIIVKAPKILRRSVNKHLIPAFDFLKGYLQSNERTIASIKRFPSIIADFR 180

Query: 181 QKQLNGSVRMLLDAGVPEARVMFFLFYQPRMFTMTLDRFREILEDVKKMGFDPSKSSFVQ 240
           Q QLNGS+RMLLDAGVPEA VM+FL YQPRMFTM LDRFREILEDV KMGFDP KSSFVQ
Sbjct: 181 QNQLNGSIRMLLDAGVPEANVMYFLLYQPRMFTMDLDRFREILEDVMKMGFDPLKSSFVQ 240

Query: 241 AVFAIRAMSKSTWVRKLGIYKRWGLSEEQILAAFRSHPWCMTLSEEKIMLAMDFFVNKLG 300
           AV AIRAMSKSTW RKL IYKRWGLSE++I+AAFRSHPWCMTLSEEKIML MDFFVNKLG
Sbjct: 241 AVHAIRAMSKSTWERKLNIYKRWGLSEKEIIAAFRSHPWCMTLSEEKIMLGMDFFVNKLG 300

Query: 301 WETAAIVRSPLLLSFSLKKRTIPRASVLLFLFQKGLTKKKTMLLVKPYMYTETQFLQKFV 360
           WE+AA+V+SP+LLSFSLKKRTIPRASVLLFLF+KGLTKKK M LVKPYMYTE QFLQKFV
Sbjct: 301 WESAAVVKSPVLLSFSLKKRTIPRASVLLFLFEKGLTKKKFM-LVKPYMYTELQFLQKFV 360

Query: 361 HPHLKEAPQLSKLYRENVDGGI 382
            PHL+E PQLSKLY  NVD GI
Sbjct: 361 RPHLEEVPQLSKLYHVNVDDGI 381

BLAST of Carg15965 vs. ExPASy TrEMBL
Match: A0A5B7BJP6 (Uncharacterized protein OS=Davidia involucrata OX=16924 GN=Din_038518 PE=3 SV=1)

HSP 1 Score: 340.9 bits (873), Expect = 7.0e-90
Identity = 183/382 (47.91%), Postives = 253/382 (66.23%), Query Frame = 0

Query: 1   MSNFLCKTL-LQVRHLTTISPYPKLRCVQSLHFNYSTV---ASDSHSFTVSYLINSCSLS 60
           M NF+CK+L   VR     SP  +L  +QS   +        S+ HSFTV+YLI+SC  S
Sbjct: 1   MFNFICKSLPSYVRCAVRTSPTHQLYFLQSRPLSSLESIPNTSNEHSFTVTYLIDSCGFS 60

Query: 61  PESASSASKLVSFTSPEKPDLVISFFKNHGFSIANISFIIWKYPRILLSNPNGTLLPKLE 120
           PE A SASK ++F +PEKPD V++FFKNHGF+   IS +I   P +LLS+P  TLLPK+E
Sbjct: 61  PEKALSASKYINFKTPEKPDSVLAFFKNHGFTKTQISTLIAGLPPVLLSDPKKTLLPKIE 120

Query: 121 FLYTRGASSSEVTKIISKAPKILMRSVDEHLVPAFDFLKGYLGSNERTIASIKRFPSIIA 180
           F +++G SS ++TKIIS+ P IL RS++  ++P+F+F    L SNE+T A+IKRF  ++ 
Sbjct: 121 FFHSKGISSPDITKIISRTPSILKRSLENQIIPSFNFFNNLLKSNEKTTAAIKRFAGLLL 180

Query: 181 DVHQKQLNGSVRMLLDAGVPEARVMFFLFYQPRMFTMTLDRFREILEDVKKMGFDPSKSS 240
              Q  +  ++ +L + GVPE+ ++  L YQPR F    DRF+EI+E+VKKMGF+P +  
Sbjct: 181 YDLQTYVAHNIGILREVGVPESNIVVLLTYQPRAFMTNTDRFKEIVEEVKKMGFNPVRMK 240

Query: 241 FVQAVFAIRAMSKSTWVRKLGIYKRWGLSEEQILAAFRSHPWCMTLSEEKIMLAMDFFVN 300
           FV A+ A+RAMSK TW +K+ ++K+WG SE++IL AF  HPWCM  SE+KI   MDFFVN
Sbjct: 241 FVIAIHALRAMSKLTWEKKVEVFKKWGWSEDEILVAFGKHPWCMMASEDKITGVMDFFVN 300

Query: 301 KLGWETAAIVRSPLLLSFSLKKRTIPRASVLLFLFQKGLTKKKTMLLVKPYMYTETQFLQ 360
           K+GWE++ + R P L+S SL+KR +PR  V   L  KGL  KK   L       E  FL+
Sbjct: 301 KMGWESSIVARRPGLISLSLEKRIVPRCLVYQDLLSKGLI-KKDFSLATMLECPEKLFLK 360

Query: 361 KFVHPHLKEAPQLSKLYRENVD 379
           K V    +EAP++ KLY+E +D
Sbjct: 361 KVVRCCKEEAPEVLKLYQEKLD 381

BLAST of Carg15965 vs. TAIR 10
Match: AT5G07900.1 (Mitochondrial transcription termination factor family protein )

HSP 1 Score: 241.1 bits (614), Expect = 1.5e-63
Identity = 132/335 (39.40%), Postives = 203/335 (60.60%), Query Frame = 0

Query: 43  SFTVSYLINSCSLSPESASSASKLVSFTSPEKPDLVISFFKNHGFSIANISFIIWKYPRI 102
           SFT++YLI+SC LSP+SA+ AS+ +   SPE+P+ V++  ++HGF+ A IS ++ K P +
Sbjct: 69  SFTLNYLIDSCGLSPDSATVASRKLLLDSPERPNTVLNLLRDHGFTTAQISSLVKKRPVL 128

Query: 103 LLSNPNGTLLPKLEFLYTRGASSSEVTKIISKAPKILMRSVDEHLVPAFDFLKGYLGSNE 162
           LL+N    LLPKL F  + G S S + + ++  P IL RS+   L+P+++FLK  L S+E
Sbjct: 129 LLANAESVLLPKLSFFLSIGVSKSLLARTLASDPTILTRSLVNQLIPSYNFLKSVLDSDE 188

Query: 163 RTIASIKRFPSIIADVHQKQLNGSVRMLLDAGVPEARVMFFLFYQPRMFTMTLDRFREIL 222
           + +A+++R   +  + H K L  ++  + + GVPE  +   L + P         F+ I 
Sbjct: 189 KIVAALRRTTWVFLEDHTKNLVPNINYMAETGVPEKCIKLLLTHFPEAVMQKNHEFQAIA 248

Query: 223 EDVKKMGFDPSKSSFVQAVFAIRAM-SKSTWVRKLGIYKRWGLSEEQILAAFRSHPWCMT 282
           +  ++MGF+P KS+FV A+ A+    +KS W +   +Y+RWG SE+ I+ AF+ HP CM 
Sbjct: 249 KQAQEMGFNPQKSTFVLAIHALSGKGNKSIWDKCFEVYQRWGWSEDDIMCAFKKHPHCMM 308

Query: 283 LSEEKIMLAMDFFVNKLGWETAAIVRSPLLLSFSLKKRTIPRASVLLFLFQKGLTKKK-- 342
           LSE KI   M++FVN++     +I + P++L FSL+KR IPR SV   L   GL K+   
Sbjct: 309 LSERKINRTMEYFVNEMNMAPRSIAQCPVVLFFSLEKRIIPRCSVTKVLVSNGLVKEDWS 368

Query: 343 -TMLLVKPYMYTETQFLQKFVHPHLKEAPQLSKLY 374
            T LLV      E  FL+K V  + +E P+L  LY
Sbjct: 369 LTSLLVP----VEKVFLEKLVIKYEEELPELMNLY 399

BLAST of Carg15965 vs. TAIR 10
Match: AT1G21150.1 (Mitochondrial transcription termination factor family protein )

HSP 1 Score: 212.6 bits (540), Expect = 5.5e-55
Identity = 122/334 (36.53%), Postives = 188/334 (56.29%), Query Frame = 0

Query: 43  SFTVSYLINSCSLSPESASSASKLVSFTSPEKPDLVISFFKNHGFSIANISFIIWKYPRI 102
           SFTVSYL++SC LS ESA S S+ V   S +KPD V++ FK+HGF+   I+ +I  +PR+
Sbjct: 56  SFTVSYLVDSCGLSLESAKSNSRFVKLVSSKKPDSVLALFKDHGFTNDQITSVIKSFPRV 115

Query: 103 LLSNPNGTLLPKLEFLYTRGASSSEVTKIISKAPKILMRSVDEHLVPAFDFLKGYLGSNE 162
           L  +P   + PKL F  + G S+S+  K+IS +PK+L  S+ + L+P +D LK  L   E
Sbjct: 116 LSLSPEDVISPKLMFFSSIGFSTSDTAKMISSSPKMLSYSLHKRLIPCYDSLKSILVEEE 175

Query: 163 RTIASIKRFPSIIADVHQKQLNGSVRMLLDAGVPEARVMFFLFYQPRMFTMTLDRFREIL 222
             +  +KR     +      ++  V +  + GVP+  + + +   P  F     RF E+L
Sbjct: 176 SVVKCLKRGIRCFSLKITHCVSLRVSICRELGVPDKSIKWLVQASPFTFFSRERRFNEVL 235

Query: 223 EDVKKMGFDPSKSSFVQAVFAIRAMSKSTWVRKLGIYKRWGLSEEQILAAFRSHPWCMTL 282
             V   GFDP K+ FV A+ A    S+S   RK  +++ +G S+E  +AA    P C+T+
Sbjct: 236 NRVCSYGFDPKKAGFVHAMVAFDCTSESAMERKFKLFQCFGWSKEDFVAAIMRFPNCVTV 295

Query: 283 SEEKIMLAMDFFVNKLGWETAAIVRSPLLLSFSLKKRTIPRASVLLFLFQKGLTKKKTML 342
           S+EKIM  +++ VN +G +   IV  P++LS S++KR  PR  V+  L  KGL KK+ + 
Sbjct: 296 SDEKIMYTLEYLVNNIGLQARDIVARPVVLSLSMEKRIKPRNQVISLLLSKGLVKKEDIN 355

Query: 343 LVKPYMYTETQFLQKFVHPHLKEAPQLSKLYREN 377
                    ++F+ KFV  +  E PQL + +  N
Sbjct: 356 YFTILKLKSSEFMDKFVLKYQDEMPQLVQPFTSN 389

BLAST of Carg15965 vs. TAIR 10
Match: AT1G61980.1 (Mitochondrial transcription termination factor family protein )

HSP 1 Score: 176.0 bits (445), Expect = 5.7e-44
Identity = 117/362 (32.32%), Postives = 191/362 (52.76%), Query Frame = 0

Query: 37  VASDSHSFTVSYLINSCSLSPESASSASKLVSFTSPEKPDLVISFFKNHGFSIANISFII 96
           V     SFTVSYL++S  L  + A S S+ VSF   + PD V++  ++HGF+ + IS I+
Sbjct: 46  VGRKGKSFTVSYLVDSLGLPKKLAESVSRKVSFEDKDNPDSVLNLLRSHGFTDSQISTIV 105

Query: 97  WKYPRILLSNPNGTLLPKLEFLYTRGASSSEVTKIISKAPKILMRSVDEHLVPAFDFLK- 156
             YP++L+++   +L PKL+FL +RGASSSE+T+I+S  PKIL +   + +   +DF+K 
Sbjct: 106 TDYPQLLVADAEKSLAPKLQFLQSRGASSSELTEIVSTVPKILGKRGHKTISVFYDFIKE 165

Query: 157 GYLGSNERTIASIKRFPSIIADVHQKQLNGSVRMLLDAGVPEARVMFFLFYQPRMFTMTL 216
             L  + ++  S + FP    ++  K  N SV  L + G+P  +++F L     +     
Sbjct: 166 TLLDKSSKSEKSCQPFPQ--GNLENKIRNLSV--LRELGMPH-KLLFPLLISCDVPVFGK 225

Query: 217 DRFREILEDVKKMGFDPSKSSFVQAVFAIRAMSKSTWVRKLGIYKRWGLSEEQILAAFRS 276
           ++F E L+ V +MGFDPS S FV+A+  ++ +S      K+  YKR G   E +   F+ 
Sbjct: 226 EKFEESLKKVVEMGFDPSTSKFVEALCVVQRLSDKNIEDKVNAYKRLGFDVEYVWTVFKR 285

Query: 277 HPWCMTLSEEKIMLAM-----------------------------------DFFVNKLGW 336
            P  +T SE+KI+  +                                   +F V K+ W
Sbjct: 286 WPNFLTHSEKKILNTIETFLGLGFSRDEFSVLIKRFPQGIGLSAEMVKKKTEFLVKKMNW 345

Query: 337 ETAAIVRSPLLLSFSLKKRTIPRASVLLFLFQKGLTKKKTMLLVKPYMYTETQFLQKFVH 363
              A+V +P +L +SL+KRT+PR +V+  L  KGL   +   + + ++ T+  FL ++V 
Sbjct: 346 PLKALVSNPAVLGYSLEKRTVPRGNVVQALISKGLIGSELPSISRVFVCTDQVFLNRYVK 402

BLAST of Carg15965 vs. TAIR 10
Match: AT1G61970.1 (Mitochondrial transcription termination factor family protein )

HSP 1 Score: 168.7 bits (426), Expect = 9.2e-42
Identity = 117/379 (30.87%), Postives = 193/379 (50.92%), Query Frame = 0

Query: 37  VASDSHSFTVSYLINSCSLSPESASSASKLVSFTSPEKPDLVISFFKNHGFSIANISFII 96
           V    ++FTVSYL++S  L+ + A S S+ VSF     PD V++   +HGF+ + IS II
Sbjct: 46  VGRKGNNFTVSYLVDSLGLTTKLAESISRKVSFEDKNNPDSVLNLLTSHGFTGSQISTII 105

Query: 97  WKYPRILLSNPNGTLLPKLEFLYTRGASSSEVTKIISKAPKILMRSVDEHLVPAFDFLKG 156
             YP++L+++   +L PKL+FL +RGASSSE+T+I+S  P+IL +   + +   +DF+K 
Sbjct: 106 RDYPQLLIADAEKSLGPKLQFLQSRGASSSEITEIVSSVPEILGKKGHKTISVYYDFIKD 165

Query: 157 YL----GSNERTIASIKRFPSIIADVHQKQLNGSVRMLLDAGVPEARVMFFLFYQPRMFT 216
            L      NE+   S+ +      ++  K  N SV  L + G+P  +++F L        
Sbjct: 166 TLLEKSSKNEKLCHSLPQ-----GNLENKIRNVSV--LRELGMPH-KLLFSLLISDSQPV 225

Query: 217 MTLDRFREILEDVKKMGFDPSKSSFVQAVFAIRAMSKSTWVRKLGIY------------- 276
              ++F E L+ V +MGFDP+ S FV+A+  I  M++ T   K+ +Y             
Sbjct: 226 CGKEKFEETLKKVVEMGFDPTTSKFVEALQVIYKMNEKTIEEKVHLYKSLGFDVGDVWSS 285

Query: 277 -KRW---------------------GLSEEQILAAFRSHPWCMTLSEEKIMLAMDFFVNK 336
            K+W                     G S ++     +  P C+ LS E +    +F V K
Sbjct: 286 FKKWPISLRVSEKKMLDSIETFLGLGFSRDEFAKMVKHFPPCIGLSTETVKKKTEFLVKK 345

Query: 337 LGWETAAIVRSPLLLSFSLKKRTIPRASVLLFLFQKGLTKKKTMLLVKPYMYTETQFLQK 375
           + W   A+V +P +  +SL+KR +PR +V+  L  KGL + +   +    M T+  FL +
Sbjct: 346 MNWPLKAVVSNPAVFGYSLEKRIVPRGNVIKALMSKGLMRNELPSISCVLMCTKQVFLNR 405

BLAST of Carg15965 vs. TAIR 10
Match: AT1G61970.2 (Mitochondrial transcription termination factor family protein )

HSP 1 Score: 168.7 bits (426), Expect = 9.2e-42
Identity = 117/379 (30.87%), Postives = 193/379 (50.92%), Query Frame = 0

Query: 37  VASDSHSFTVSYLINSCSLSPESASSASKLVSFTSPEKPDLVISFFKNHGFSIANISFII 96
           V    ++FTVSYL++S  L+ + A S S+ VSF     PD V++   +HGF+ + IS II
Sbjct: 46  VGRKGNNFTVSYLVDSLGLTTKLAESISRKVSFEDKNNPDSVLNLLTSHGFTGSQISTII 105

Query: 97  WKYPRILLSNPNGTLLPKLEFLYTRGASSSEVTKIISKAPKILMRSVDEHLVPAFDFLKG 156
             YP++L+++   +L PKL+FL +RGASSSE+T+I+S  P+IL +   + +   +DF+K 
Sbjct: 106 RDYPQLLIADAEKSLGPKLQFLQSRGASSSEITEIVSSVPEILGKKGHKTISVYYDFIKD 165

Query: 157 YL----GSNERTIASIKRFPSIIADVHQKQLNGSVRMLLDAGVPEARVMFFLFYQPRMFT 216
            L      NE+   S+ +      ++  K  N SV  L + G+P  +++F L        
Sbjct: 166 TLLEKSSKNEKLCHSLPQ-----GNLENKIRNVSV--LRELGMPH-KLLFSLLISDSQPV 225

Query: 217 MTLDRFREILEDVKKMGFDPSKSSFVQAVFAIRAMSKSTWVRKLGIY------------- 276
              ++F E L+ V +MGFDP+ S FV+A+  I  M++ T   K+ +Y             
Sbjct: 226 CGKEKFEETLKKVVEMGFDPTTSKFVEALQVIYKMNEKTIEEKVHLYKSLGFDVGDVWSS 285

Query: 277 -KRW---------------------GLSEEQILAAFRSHPWCMTLSEEKIMLAMDFFVNK 336
            K+W                     G S ++     +  P C+ LS E +    +F V K
Sbjct: 286 FKKWPISLRVSEKKMLDSIETFLGLGFSRDEFAKMVKHFPPCIGLSTETVKKKTEFLVKK 345

Query: 337 LGWETAAIVRSPLLLSFSLKKRTIPRASVLLFLFQKGLTKKKTMLLVKPYMYTETQFLQK 375
           + W   A+V +P +  +SL+KR +PR +V+  L  KGL + +   +    M T+  FL +
Sbjct: 346 MNWPLKAVVSNPAVFGYSLEKRIVPRGNVIKALMSKGLMRNELPSISCVLMCTKQVFLNR 405

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
KAG7026295.15.0e-215100.00Transcription termination factor MTERF8, chloroplastic, partial [Cucurbita argyr... [more]
KAG6593956.13.0e-21299.22Transcription termination factor MTERF8, chloroplastic, partial [Cucurbita argyr... [more]
XP_022930344.12.0e-21198.70transcription termination factor MTERF6, chloroplastic/mitochondrial-like [Cucur... [more]
XP_023514950.11.4e-20496.11transcription termination factor MTERF6, chloroplastic/mitochondrial-like isofor... [more]
XP_022930343.11.2e-19793.01transcription termination factor MTERF6, chloroplastic/mitochondrial-like [Cucur... [more]
Match NameE-valueIdentityDescription
Q9FK234.2e-1524.81Transcription termination factor MTERF8, chloroplastic OS=Arabidopsis thaliana O... [more]
Q9SZL63.4e-0922.74Transcription termination factor MTERF6, chloroplastic/mitochondrial OS=Arabidop... [more]
Q84X531.0e-0526.74Transcription termination factor MTEF1, chloroplastic OS=Arabidopsis thaliana OX... [more]
Match NameE-valueIdentityDescription
A0A6J1ER699.5e-21298.70transcription termination factor MTERF6, chloroplastic/mitochondrial-like OS=Cuc... [more]
A0A6J1EUW15.9e-19893.01transcription termination factor MTERF6, chloroplastic/mitochondrial-like OS=Cuc... [more]
A0A6J1KHT41.1e-19693.70transcription termination factor MTERF6, chloroplastic/mitochondrial-like OS=Cuc... [more]
A0A6J1CAW86.2e-17182.46transcription termination factor MTERF6, chloroplastic/mitochondrial-like OS=Mom... [more]
A0A5B7BJP67.0e-9047.91Uncharacterized protein OS=Davidia involucrata OX=16924 GN=Din_038518 PE=3 SV=1[more]
Match NameE-valueIdentityDescription
AT5G07900.11.5e-6339.40Mitochondrial transcription termination factor family protein [more]
AT1G21150.15.5e-5536.53Mitochondrial transcription termination factor family protein [more]
AT1G61980.15.7e-4432.32Mitochondrial transcription termination factor family protein [more]
AT1G61970.19.2e-4230.87Mitochondrial transcription termination factor family protein [more]
AT1G61970.29.2e-4230.87Mitochondrial transcription termination factor family protein [more]
InterPro
Analysis Name: InterPro Annotations of Silver-seed gourd (SMH-JMG-627) v2
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR003690Transcription termination factor, mitochondrial/chloroplasticSMARTSM00733mt_12coord: 129..161
e-value: 0.0039
score: 26.4
coord: 302..336
e-value: 160.0
score: 10.2
coord: 93..124
e-value: 0.12
score: 21.5
coord: 165..196
e-value: 1900.0
score: 1.7
coord: 270..301
e-value: 0.074
score: 22.2
coord: 201..231
e-value: 1800.0
score: 1.9
IPR003690Transcription termination factor, mitochondrial/chloroplasticPFAMPF02536mTERFcoord: 79..235
e-value: 7.4E-21
score: 74.4
coord: 179..338
e-value: 1.9E-15
score: 56.6
IPR003690Transcription termination factor, mitochondrial/chloroplasticPANTHERPTHR13068CGI-12 PROTEIN-RELATEDcoord: 24..379
IPR038538MTERF superfamily, mitochondrial/chloroplasticGENE3D1.25.70.10Transcription termination factor 3, mitochondrialcoord: 160..371
e-value: 9.9E-38
score: 131.8
NoneNo IPR availablePANTHERPTHR13068:SF160TRANSCRIPTION TERMINATION FACTOR FAMILY PROTEIN, PUTATIVE ISOFORM 1-RELATEDcoord: 24..379

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Carg15965-RACarg15965-RAmRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0006353 DNA-templated transcription, termination
biological_process GO:0006355 regulation of transcription, DNA-templated
molecular_function GO:0003690 double-stranded DNA binding