Carg15927 (gene) Silver-seed gourd (SMH-JMG-627) v2

Overview


Name	Carg15927
Type	gene
Organism	Cucurbita argyrosperma subsp. argyrosperma cv. SMH-JMG-627 (Silver-seed gourd (SMH-JMG-627) v2)
Description	adenylate isopentenyltransferase 5, chloroplastic-like
Location	Carg_Chr08: 7163957 .. 7164931 (+)
RNA-Seq Expression	Carg15927
Synteny	Carg15927

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGAAAATTTCCTTGTCGGCGAGCTGCAAGAAAGCTGCGGCGGAGCCGCCGCTAATAGACTTCCAAGGAACAAACACCATCAATTTGAAGCCATTTTTCCGGCGGAAAAACAAATTAGTGTTTGTAATGGGTGCGACCGGAACTGGAAAGTCCCGGTTGGCCGTCGATTTAGCCACCCGTTTCCCAGCTGAAATCGTAAACTCCGACAAAATCCAAATCTACAAAGGACTCGAAGTCGTCACCAATAAGGTAACCGAAAAAGAATGCTGTGGTGTCCCACATCACCTCCTTGGAATTGCCGATCCAAACTCCAACTTCACCGCCGCAGACTTCCGCCGCCACGCCACATCAACGATAAACTCCATCGTTGGAAAACACCGGCTCCCCATCGTGGCCGGCGGCTCCAACTCCTTCATCGAATCTCTTGTTGACGGCGACGGCGAGTTCCGATACCAGTACGAATGTTGCTTCTTATGGGTCGACGTGTCATTACCAATCCTACACTCCTTCGTGTCGGAACGCGTCGATCGGATGTTGGACAACGGCTTCATTAACGAGGTACGGAATTTATTCGATCCTAATGAAAACGATTACACGCGTGGGATCCGACGCGCCATTGGAGTTTCTGAACTCGACGCGTTTCTACGCGCCGAGATGGATTCCGCCACCGATTACAGAGCGCGATCTAGAATTCTCGAAGCGGCCATTTTAAAGATAAAGGAAAACACCAGAAAATTGGCATTCCGACAGTGGCAGAACATCCTCCGCCTTCGGAGCAAACGGGGATGGAATCTCCACCGCATTGACGCCACCAAAGTCTTCCAACACCGTGACAACAAACAACATTCCGACCATGTTTGGGAGGCGCTCGTCACCGAGCCGGCCTCCAAAATCCTTCACCAATTCCTATGTCAAAATACTTACACCGTCAATATTCCGACCACCACCATTAAATCAAATTTAACTGTCTTTTAA

mRNA sequence

ATGAAAATTTCCTTGTCGGCGAGCTGCAAGAAAGCTGCGGCGGAGCCGCCGCTAATAGACTTCCAAGGAACAAACACCATCAATTTGAAGCCATTTTTCCGGCGGAAAAACAAATTAGTGTTTGTAATGGGTGCGACCGGAACTGGAAAGTCCCGGTTGGCCGTCGATTTAGCCACCCGTTTCCCAGCTGAAATCGTAAACTCCGACAAAATCCAAATCTACAAAGGACTCGAAGTCGTCACCAATAAGGTAACCGAAAAAGAATGCTGTGGTGTCCCACATCACCTCCTTGGAATTGCCGATCCAAACTCCAACTTCACCGCCGCAGACTTCCGCCGCCACGCCACATCAACGATAAACTCCATCGTTGGAAAACACCGGCTCCCCATCGTGGCCGGCGGCTCCAACTCCTTCATCGAATCTCTTGTTGACGGCGACGGCGAGTTCCGATACCAGTACGAATGTTGCTTCTTATGGGTCGACGTGTCATTACCAATCCTACACTCCTTCGTGTCGGAACGCGTCGATCGGATGTTGGACAACGGCTTCATTAACGAGGTACGGAATTTATTCGATCCTAATGAAAACGATTACACGCGTGGGATCCGACGCGCCATTGGAGTTTCTGAACTCGACGCGTTTCTACGCGCCGAGATGGATTCCGCCACCGATTACAGAGCGCGATCTAGAATTCTCGAAGCGGCCATTTTAAAGATAAAGGAAAACACCAGAAAATTGGCATTCCGACAGTGGCAGAACATCCTCCGCCTTCGGAGCAAACGGGGATGGAATCTCCACCGCATTGACGCCACCAAAGTCTTCCAACACCGTGACAACAAACAACATTCCGACCATGTTTGGGAGGCGCTCGTCACCGAGCCGGCCTCCAAAATCCTTCACCAATTCCTATGTCAAAATACTTACACCGTCAATATTCCGACCACCACCATTAAATCAAATTTAACTGTCTTTTAA

Coding sequence (CDS)

ATGAAAATTTCCTTGTCGGCGAGCTGCAAGAAAGCTGCGGCGGAGCCGCCGCTAATAGACTTCCAAGGAACAAACACCATCAATTTGAAGCCATTTTTCCGGCGGAAAAACAAATTAGTGTTTGTAATGGGTGCGACCGGAACTGGAAAGTCCCGGTTGGCCGTCGATTTAGCCACCCGTTTCCCAGCTGAAATCGTAAACTCCGACAAAATCCAAATCTACAAAGGACTCGAAGTCGTCACCAATAAGGTAACCGAAAAAGAATGCTGTGGTGTCCCACATCACCTCCTTGGAATTGCCGATCCAAACTCCAACTTCACCGCCGCAGACTTCCGCCGCCACGCCACATCAACGATAAACTCCATCGTTGGAAAACACCGGCTCCCCATCGTGGCCGGCGGCTCCAACTCCTTCATCGAATCTCTTGTTGACGGCGACGGCGAGTTCCGATACCAGTACGAATGTTGCTTCTTATGGGTCGACGTGTCATTACCAATCCTACACTCCTTCGTGTCGGAACGCGTCGATCGGATGTTGGACAACGGCTTCATTAACGAGGTACGGAATTTATTCGATCCTAATGAAAACGATTACACGCGTGGGATCCGACGCGCCATTGGAGTTTCTGAACTCGACGCGTTTCTACGCGCCGAGATGGATTCCGCCACCGATTACAGAGCGCGATCTAGAATTCTCGAAGCGGCCATTTTAAAGATAAAGGAAAACACCAGAAAATTGGCATTCCGACAGTGGCAGAACATCCTCCGCCTTCGGAGCAAACGGGGATGGAATCTCCACCGCATTGACGCCACCAAAGTCTTCCAACACCGTGACAACAAACAACATTCCGACCATGTTTGGGAGGCGCTCGTCACCGAGCCGGCCTCCAAAATCCTTCACCAATTCCTATGTCAAAATACTTACACCGTCAATATTCCGACCACCACCATTAAATCAAATTTAACTGTCTTTTAA

Protein sequence

MKISLSASCKKAAAEPPLIDFQGTNTINLKPFFRRKNKLVFVMGATGTGKSRLAVDLATRFPAEIVNSDKIQIYKGLEVVTNKVTEKECCGVPHHLLGIADPNSNFTAADFRRHATSTINSIVGKHRLPIVAGGSNSFIESLVDGDGEFRYQYECCFLWVDVSLPILHSFVSERVDRMLDNGFINEVRNLFDPNENDYTRGIRRAIGVSELDAFLRAEMDSATDYRARSRILEAAILKIKENTRKLAFRQWQNILRLRSKRGWNLHRIDATKVFQHRDNKQHSDHVWEALVTEPASKILHQFLCQNTYTVNIPTTTIKSNLTVF

Homology

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BLAST of Carg15927 vs. NCBI nr
Match: KAG7026259.1 (Adenylate isopentenyltransferase 5, chloroplastic, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 655.2 bits (1689), Expect = 2.9e-184
Identity = 324/324 (100.00%), Postives = 324/324 (100.00%), Query Frame = 0


Query: 1   MKISLSASCKKAAAEPPLIDFQGTNTINLKPFFRRKNKLVFVMGATGTGKSRLAVDLATR 60
           MKISLSASCKKAAAEPPLIDFQGTNTINLKPFFRRKNKLVFVMGATGTGKSRLAVDLATR
Sbjct: 1   MKISLSASCKKAAAEPPLIDFQGTNTINLKPFFRRKNKLVFVMGATGTGKSRLAVDLATR 60

Query: 61  FPAEIVNSDKIQIYKGLEVVTNKVTEKECCGVPHHLLGIADPNSNFTAADFRRHATSTIN 120
           FPAEIVNSDKIQIYKGLEVVTNKVTEKECCGVPHHLLGIADPNSNFTAADFRRHATSTIN
Sbjct: 61  FPAEIVNSDKIQIYKGLEVVTNKVTEKECCGVPHHLLGIADPNSNFTAADFRRHATSTIN 120

Query: 121 SIVGKHRLPIVAGGSNSFIESLVDGDGEFRYQYECCFLWVDVSLPILHSFVSERVDRMLD 180
           SIVGKHRLPIVAGGSNSFIESLVDGDGEFRYQYECCFLWVDVSLPILHSFVSERVDRMLD
Sbjct: 121 SIVGKHRLPIVAGGSNSFIESLVDGDGEFRYQYECCFLWVDVSLPILHSFVSERVDRMLD 180

Query: 181 NGFINEVRNLFDPNENDYTRGIRRAIGVSELDAFLRAEMDSATDYRARSRILEAAILKIK 240
           NGFINEVRNLFDPNENDYTRGIRRAIGVSELDAFLRAEMDSATDYRARSRILEAAILKIK
Sbjct: 181 NGFINEVRNLFDPNENDYTRGIRRAIGVSELDAFLRAEMDSATDYRARSRILEAAILKIK 240

Query: 241 ENTRKLAFRQWQNILRLRSKRGWNLHRIDATKVFQHRDNKQHSDHVWEALVTEPASKILH 300
           ENTRKLAFRQWQNILRLRSKRGWNLHRIDATKVFQHRDNKQHSDHVWEALVTEPASKILH
Sbjct: 241 ENTRKLAFRQWQNILRLRSKRGWNLHRIDATKVFQHRDNKQHSDHVWEALVTEPASKILH 300

Query: 301 QFLCQNTYTVNIPTTTIKSNLTVF 325
           QFLCQNTYTVNIPTTTIKSNLTVF
Sbjct: 301 QFLCQNTYTVNIPTTTIKSNLTVF 324

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BLAST of Carg15927 vs. NCBI nr
Match: KAG6593917.1 (Adenylate isopentenyltransferase 5, chloroplastic, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 644.0 bits (1660), Expect = 6.7e-181
Identity = 319/324 (98.46%), Postives = 321/324 (99.07%), Query Frame = 0


Query: 1   MKISLSASCKKAAAEPPLIDFQGTNTINLKPFFRRKNKLVFVMGATGTGKSRLAVDLATR 60
           MKISLSASCKKAAAEPPLIDFQGTNTINLKPFFRRKNKLVFVMGATGTGKSRLAVDLATR
Sbjct: 1   MKISLSASCKKAAAEPPLIDFQGTNTINLKPFFRRKNKLVFVMGATGTGKSRLAVDLATR 60

Query: 61  FPAEIVNSDKIQIYKGLEVVTNKVTEKECCGVPHHLLGIADPNSNFTAADFRRHATSTIN 120
           F AEIVNSDKIQIYKGLEVVTNKVTEKECCGVPHHLLGIADPNSNFTAADFRRHATSTIN
Sbjct: 61  FSAEIVNSDKIQIYKGLEVVTNKVTEKECCGVPHHLLGIADPNSNFTAADFRRHATSTIN 120

Query: 121 SIVGKHRLPIVAGGSNSFIESLVDGDGEFRYQYECCFLWVDVSLPILHSFVSERVDRMLD 180
           SIVGKHRLPIVAGGSNSFIESLVDGDGEFRYQYECCFLWVDVSLPILHSFVSERVDRMLD
Sbjct: 121 SIVGKHRLPIVAGGSNSFIESLVDGDGEFRYQYECCFLWVDVSLPILHSFVSERVDRMLD 180

Query: 181 NGFINEVRNLFDPNENDYTRGIRRAIGVSELDAFLRAEMDSATDYRARSRILEAAILKIK 240
           NGFINEVRNLFDPNENDYTRGIRRAIGV ELDAFLRAEMDSATDYRARSRILEAAILKIK
Sbjct: 181 NGFINEVRNLFDPNENDYTRGIRRAIGVPELDAFLRAEMDSATDYRARSRILEAAILKIK 240

Query: 241 ENTRKLAFRQWQNILRLRSKRGWNLHRIDATKVFQHRDNKQHSDHVWEALVTEPASKILH 300
           ENTRKLAFRQWQNI RLRSKRGWNL+RIDAT+VFQHRDNKQHSDHVWEALVTEPASKILH
Sbjct: 241 ENTRKLAFRQWQNIRRLRSKRGWNLYRIDATEVFQHRDNKQHSDHVWEALVTEPASKILH 300

Query: 301 QFLCQNTYTVNIPTTTIKSNLTVF 325
           QFLCQNTYTVNIPTTTIKSNLTVF
Sbjct: 301 QFLCQNTYTVNIPTTTIKSNLTVF 324

[Close]

BLAST of Carg15927 vs. NCBI nr
Match: XP_023522124.1 (adenylate isopentenyltransferase 5, chloroplastic-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 641.3 bits (1653), Expect = 4.4e-180
Identity = 317/324 (97.84%), Postives = 320/324 (98.77%), Query Frame = 0


Query: 1   MKISLSASCKKAAAEPPLIDFQGTNTINLKPFFRRKNKLVFVMGATGTGKSRLAVDLATR 60
           MKISLSASCKKAAAEPPLIDFQGTNTINLKPFFRRK+KLVFVMGATGTGKSRLAVDLATR
Sbjct: 1   MKISLSASCKKAAAEPPLIDFQGTNTINLKPFFRRKDKLVFVMGATGTGKSRLAVDLATR 60

Query: 61  FPAEIVNSDKIQIYKGLEVVTNKVTEKECCGVPHHLLGIADPNSNFTAADFRRHATSTIN 120
           FPAEIVNSDKIQIYKGLEVVTNKVTEKECCGVPHHLLGIADPNSNFTAADFRRHATSTIN
Sbjct: 61  FPAEIVNSDKIQIYKGLEVVTNKVTEKECCGVPHHLLGIADPNSNFTAADFRRHATSTIN 120

Query: 121 SIVGKHRLPIVAGGSNSFIESLVDGDGEFRYQYECCFLWVDVSLPILHSFVSERVDRMLD 180
           SIVGKHRLPIVAGGSNSFIESLVDGD EFRYQYECCFLWVDVSLP+LHSFVSERVDRMLD
Sbjct: 121 SIVGKHRLPIVAGGSNSFIESLVDGDAEFRYQYECCFLWVDVSLPVLHSFVSERVDRMLD 180

Query: 181 NGFINEVRNLFDPNENDYTRGIRRAIGVSELDAFLRAEMDSATDYRARSRILEAAILKIK 240
           NGFINEVRNLFD NENDYTRGIRRAIGV ELDAFLRAEMDSATDYRARSRILEAAILKIK
Sbjct: 181 NGFINEVRNLFDRNENDYTRGIRRAIGVPELDAFLRAEMDSATDYRARSRILEAAILKIK 240

Query: 241 ENTRKLAFRQWQNILRLRSKRGWNLHRIDATKVFQHRDNKQHSDHVWEALVTEPASKILH 300
           ENTRKLAFRQWQNI RLRSKRGWNLHRIDAT+VFQHRDNKQHSDHVWEALVTEPASKILH
Sbjct: 241 ENTRKLAFRQWQNIRRLRSKRGWNLHRIDATEVFQHRDNKQHSDHVWEALVTEPASKILH 300

Query: 301 QFLCQNTYTVNIPTTTIKSNLTVF 325
           QFLCQNTYTVNIPTTTIKSNLTVF
Sbjct: 301 QFLCQNTYTVNIPTTTIKSNLTVF 324

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BLAST of Carg15927 vs. NCBI nr
Match: XP_022930300.1 (adenylate isopentenyltransferase 5, chloroplastic-like [Cucurbita moschata])

HSP 1 Score: 639.0 bits (1647), Expect = 2.2e-179
Identity = 315/324 (97.22%), Postives = 319/324 (98.46%), Query Frame = 0


Query: 1   MKISLSASCKKAAAEPPLIDFQGTNTINLKPFFRRKNKLVFVMGATGTGKSRLAVDLATR 60
           MKISLSASCKKAAAEPPLIDFQGTNT+NLKPFFR+K+KLVFVMGATGTGKSRLAVDLATR
Sbjct: 1   MKISLSASCKKAAAEPPLIDFQGTNTVNLKPFFRQKDKLVFVMGATGTGKSRLAVDLATR 60

Query: 61  FPAEIVNSDKIQIYKGLEVVTNKVTEKECCGVPHHLLGIADPNSNFTAADFRRHATSTIN 120
           FPAEIVNSDKIQIYKGLEVVTNKVTEKECCGVPHHLLGIADPNSNFT ADFRRHATSTIN
Sbjct: 61  FPAEIVNSDKIQIYKGLEVVTNKVTEKECCGVPHHLLGIADPNSNFTFADFRRHATSTIN 120

Query: 121 SIVGKHRLPIVAGGSNSFIESLVDGDGEFRYQYECCFLWVDVSLPILHSFVSERVDRMLD 180
           SIVGKHRLPIVAGGSNSFIESLVDGD EFRYQYECCFLWVDVSLPILHSFVSERVDRMLD
Sbjct: 121 SIVGKHRLPIVAGGSNSFIESLVDGDAEFRYQYECCFLWVDVSLPILHSFVSERVDRMLD 180

Query: 181 NGFINEVRNLFDPNENDYTRGIRRAIGVSELDAFLRAEMDSATDYRARSRILEAAILKIK 240
           NGFINEVRNLFDPNENDYTRGIRRAIGV ELDAFLRAEMDSATDYRARSRILEAAILKIK
Sbjct: 181 NGFINEVRNLFDPNENDYTRGIRRAIGVPELDAFLRAEMDSATDYRARSRILEAAILKIK 240

Query: 241 ENTRKLAFRQWQNILRLRSKRGWNLHRIDATKVFQHRDNKQHSDHVWEALVTEPASKILH 300
           ENTRKLAFRQWQNI RLRSKRGWNLHRIDAT+VFQHRDNKQHSDHVWEALVTEPASKILH
Sbjct: 241 ENTRKLAFRQWQNIRRLRSKRGWNLHRIDATEVFQHRDNKQHSDHVWEALVTEPASKILH 300

Query: 301 QFLCQNTYTVNIPTTTIKSNLTVF 325
           QFLCQNTYTVNIPTT IKSNLTVF
Sbjct: 301 QFLCQNTYTVNIPTTAIKSNLTVF 324

[Close]

BLAST of Carg15927 vs. NCBI nr
Match: XP_023513929.1 (adenylate isopentenyltransferase 5, chloroplastic-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 635.2 bits (1637), Expect = 3.1e-178
Identity = 315/324 (97.22%), Postives = 319/324 (98.46%), Query Frame = 0


Query: 1   MKISLSASCKKAAAEPPLIDFQGTNTINLKPFFRRKNKLVFVMGATGTGKSRLAVDLATR 60
           MKISLSASCKKAAAEPPLIDFQGTN+INLKPFFRRK+KLVFVMGATGTGKSRLAVDLATR
Sbjct: 1   MKISLSASCKKAAAEPPLIDFQGTNSINLKPFFRRKDKLVFVMGATGTGKSRLAVDLATR 60

Query: 61  FPAEIVNSDKIQIYKGLEVVTNKVTEKECCGVPHHLLGIADPNSNFTAADFRRHATSTIN 120
           FPAEIVNSDKIQIYKGLEVVTNKVTEKEC GVPHHLLGIADPNSNFTAADFRRHATSTIN
Sbjct: 61  FPAEIVNSDKIQIYKGLEVVTNKVTEKECRGVPHHLLGIADPNSNFTAADFRRHATSTIN 120

Query: 121 SIVGKHRLPIVAGGSNSFIESLVDGDGEFRYQYECCFLWVDVSLPILHSFVSERVDRMLD 180
           SIVGKHRLPIVAGGSNSFIESLVDGD EFRYQYECCFLWVDVSLP+LHSFVSERVDRMLD
Sbjct: 121 SIVGKHRLPIVAGGSNSFIESLVDGDAEFRYQYECCFLWVDVSLPVLHSFVSERVDRMLD 180

Query: 181 NGFINEVRNLFDPNENDYTRGIRRAIGVSELDAFLRAEMDSATDYRARSRILEAAILKIK 240
           NGFINEVRNLFD NENDYTRGIRRAIGV ELDAFLRAEMDSATDYRARSRILEAAILKIK
Sbjct: 181 NGFINEVRNLFDRNENDYTRGIRRAIGVPELDAFLRAEMDSATDYRARSRILEAAILKIK 240

Query: 241 ENTRKLAFRQWQNILRLRSKRGWNLHRIDATKVFQHRDNKQHSDHVWEALVTEPASKILH 300
           ENTRKLAFRQWQNI RLRSKRGWNLHRIDAT+VFQHRDNKQHSDHVWEALVTEPASKILH
Sbjct: 241 ENTRKLAFRQWQNIRRLRSKRGWNLHRIDATEVFQHRDNKQHSDHVWEALVTEPASKILH 300

Query: 301 QFLCQNTYTVNIPTTTIKSNLTVF 325
           QFLCQNTYTVNIPTTTIKSNLTVF
Sbjct: 301 QFLCQNTYTVNIPTTTIKSNLTVF 324

[Close]

BLAST of Carg15927 vs. ExPASy Swiss-Prot
Match: Q94ID2 (Adenylate isopentenyltransferase 5, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=IPT5 PE=1 SV=2)

HSP 1 Score: 336.3 bits (861), Expect = 3.9e-91
Identity = 174/292 (59.59%), Postives = 223/292 (76.37%), Query Frame = 0


Query: 15  EPPLIDFQGTNTINLKPFFRRKNKLVFVMGATGTGKSRLAVDLATRFPAEIVNSDKIQIY 74
           +P  ++FQG N +++ PFFRRK+K+VFVMGATGTGKSRLA+DLATRFPAEIVNSDKIQ+Y
Sbjct: 13  QPLSLNFQG-NMVDV-PFFRRKDKVVFVMGATGTGKSRLAIDLATRFPAEIVNSDKIQVY 72

Query: 75  KGLEVVTNKVTEKECCGVPHHLLG-IADPNSNFTAADFRRHATSTINSIVGKHRLPIVAG 134
           KGL++VTNKVT +E  GVPHHLLG + D   +FTA DF+R A   + SIV + R+PI+AG
Sbjct: 73  KGLDIVTNKVTPEESLGVPHHLLGTVHDTYEDFTAEDFQREAIRAVESIVQRDRVPIIAG 132

Query: 135 GSNSFIESLVDGDGEFRYQYECCFLWVDVSLPILHSFVSERVDRMLDNGFINEVRNLFDP 194
           GSNS+IE+LV+   +FR +Y CCFLWVDVS P+LHSFVSERVD+M+D G ++EVR +FDP
Sbjct: 133 GSNSYIEALVNDCVDFRLRYNCCFLWVDVSRPVLHSFVSERVDKMVDMGLVDEVRRIFDP 192

Query: 195 NENDYTRGIRRAIGVSELDAFLRAEMDSATDYRARS--RILEAAILKIKENTRKLAFRQW 254
           + +DY+ GIRRAIGV ELD FLR+EM    +Y A +  R+LE AI KIKENT  LA RQ 
Sbjct: 193 SSSDYSAGIRRAIGVPELDEFLRSEM---RNYPAETTERLLETAIEKIKENTCLLACRQL 252

Query: 255 QNILRLRSKRGWNLHRIDATKVFQHRDNKQHSDHVWEALVTEPASKILHQFL 304
           Q I RL  +  WN+HR+DAT+VF  R   + +D  W+  V  P++  + +FL
Sbjct: 253 QKIQRLYKQWKWNMHRVDATEVFLRRG--EEADEAWDNSVAHPSALAVEKFL 297

[Close]

BLAST of Carg15927 vs. ExPASy Swiss-Prot
Match: Q93WC9 (Adenylate isopentenyltransferase 3, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=IPT3 PE=1 SV=1)

HSP 1 Score: 302.8 bits (774), Expect = 4.8e-81
Identity = 151/308 (49.03%), Postives = 216/308 (70.13%), Query Frame = 0


Query: 1   MKISLSASCKKAAAEPPLIDFQ----GTNTINLKPFFRRKNKLVFVMGATGTGKSRLAVD 60
           MKIS+ A CK+     P +DF     G N + L P +  K+K+V +MGATGTGKSRL+VD
Sbjct: 3   MKISM-AMCKQPLPPSPTLDFPPARFGPNMLTLNP-YGPKDKVVVIMGATGTGKSRLSVD 62

Query: 61  LATRFPAEIVNSDKIQIYKGLEVVTNKVTEKECCGVPHHLLGIADPNSNFTAADFRRHAT 120
           +ATRF AEI+NSDKIQ+++GL++VTNK+T +E CGVPHHLLG+  P ++ TAA++   A 
Sbjct: 63  IATRFRAEIINSDKIQVHQGLDIVTNKITSEESCGVPHHLLGVLPPEADLTAANYCHMAN 122

Query: 121 STINSIVGKHRLPIVAGGSNSFIESLVDG-DGEFRYQYECCFLWVDVSLPILHSFVSERV 180
            +I S++ + +LPI+ GGSNS++E+LVD  + +FR +Y+CCFLWVDV+LP+LH FVSERV
Sbjct: 123 LSIESVLNRGKLPIIVGGSNSYVEALVDDKENKFRSRYDCCFLWVDVALPVLHGFVSERV 182

Query: 181 DRMLDNGFINEVRNLFDPNENDYTRGIRRAIGVSELDAFLRAEMDSATDYRARSRILEAA 240
           D+M+++G + EVR  FD + +DY+RGI++AIG  E D F R E     +   R  +L   
Sbjct: 183 DKMVESGMVEEVREFFDFSNSDYSRGIKKAIGFPEFDRFFRNE--QFLNVEDREELLSKV 242

Query: 241 ILKIKENTRKLAFRQWQNILRLRSKRGWNLHRIDATKVFQHRDNKQHSDHVWEALVTEPA 300
           + +IK NT +LA RQ + I RLR  + W++ R+DAT VF  R +K  ++  WE LV  P+
Sbjct: 243 LEEIKRNTFELACRQREKIERLRKVKKWSIQRVDATPVFTKRRSKMDANVAWERLVAGPS 302

Query: 301 SKILHQFL 304
           +  + +FL
Sbjct: 303 TDTVSRFL 306

[Close]

BLAST of Carg15927 vs. ExPASy Swiss-Prot
Match: Q94ID1 (Adenylate isopentenyltransferase 7, mitochondrial OS=Arabidopsis thaliana OX=3702 GN=IPT7 PE=2 SV=2)

HSP 1 Score: 294.3 bits (752), Expect = 1.7e-78
Identity = 160/313 (51.12%), Postives = 213/313 (68.05%), Query Frame = 0


Query: 1   MKISLSASCKKAAAEPPLIDFQGTNTINLKPFFRRKNKLVFVMGATGTGKSRLAVDLATR 60
           MK S+S S K+   +P L      + +N+  F   K K++FVMGATG+GKSRLA+DLATR
Sbjct: 1   MKFSIS-SLKQ--VQPILCFKNKLSKVNVNSFLHPKEKVIFVMGATGSGKSRLAIDLATR 60

Query: 61  FPAEIVNSDKIQIYKGLEVVTNKVTEKECCGVPHHLLGIADPNS-NFTAADFRRHATSTI 120
           F  EI+NSDKIQ+YKGL+V+TNKVT KEC GVPHHLLG+ D  + N TA  + R A+  I
Sbjct: 61  FQGEIINSDKIQLYKGLDVLTNKVTPKECRGVPHHLLGVFDSEAGNLTATQYSRLASQAI 120

Query: 121 NSIVGKHRLPIVAGGSNSFIESLVD-GDGEFRYQYECCFLWVDVSLPILHSFVSERVDRM 180
           + +   ++LPIVAGGSNS+IE+LV+   G     Y+CCF+WVDVSLP+L+SFVS+RVDRM
Sbjct: 121 SKLSANNKLPIVAGGSNSYIEALVNHSSGFLLNNYDCCFIWVDVSLPVLNSFVSKRVDRM 180

Query: 181 LDNGFINEVRNLFDPNENDYTRGIRRAIGVSELDAFLRAEMDSATDYRARSRILEAAILK 240
           ++ G + EVR +F+P  N Y+ GIRRAIGV EL  +LR E  S  D   +S++L+ A+  
Sbjct: 181 MEAGLLEEVREVFNPKAN-YSVGIRRAIGVPELHEYLRNE--SLVDRATKSKMLDVAVKN 240

Query: 241 IKENTRKLAFRQWQNILRLRSKRGWNLHRIDATKVFQHRDNKQHSDHVWEALVTEPASKI 300
           IK+NT  LA RQ + I RL  K   ++HR+DAT+VF  R N +  D  WE LV  P+ +I
Sbjct: 241 IKKNTEILACRQLKKIQRLHKKWKMSMHRVDATEVFLKR-NVEEQDEAWENLVARPSERI 300

Query: 301 LHQFLCQNTYTVN 312
           + +F   N    N
Sbjct: 301 VDKFYNNNNQLKN 306

[Close]

BLAST of Carg15927 vs. ExPASy Swiss-Prot
Match: Q5GHF7 (Adenylate isopentenyltransferase OS=Humulus lupulus OX=3486 PE=1 SV=1)

HSP 1 Score: 233.4 bits (594), Expect = 3.6e-60
Identity = 132/312 (42.31%), Postives = 186/312 (59.62%), Query Frame = 0


Query: 24  TNTINLKPFFRRKNKLVFVMGATGTGKSRLAVDLATRFPAEIVNSDKIQIYKGLEVVTNK 83
           T  ++L+    RK KL+ +MGATGTGKSRL++DLA  FP E++NSDK+Q+YKGL++ TNK
Sbjct: 17  TTNVSLRRQRHRKEKLLVLMGATGTGKSRLSIDLAAHFPLEVINSDKMQVYKGLDITTNK 76

Query: 84  VTEKECCGVPHHLLGIADP-NSNFTAADFRRHATSTINSIVGKHRLPIVAGGSNSFIESL 143
           ++  +  GVPHHLLG  DP     T ADFR  A   ++ I G+ +LP++ GGSNSFI +L
Sbjct: 77  ISVPDRGGVPHHLLGEVDPARGELTPADFRSLAGKAVSEITGRRKLPVLVGGSNSFIHAL 136

Query: 144 V------DGDGEF----------RYQYECCFLWVDVSLPILHSFVSERVDRMLDNGFINE 203
           +       G G F            +Y+CCFLWVDVS+ +L  ++++RVD ML+ G  +E
Sbjct: 137 LVDRFDSSGPGVFEEGSHSVVSSELRYDCCFLWVDVSVKVLTDYLAKRVDDMLELGMFDE 196

Query: 204 VRNLFDPNENDY-----TR-GIRRAIGVSELDAFLR------AEMDSATDYRARSRILEA 263
           +   + P + D+     TR G+R+AIGV E D +         E +     R R    E 
Sbjct: 197 LAEFYSPEDEDHDEDSATRTGLRKAIGVPEFDRYFEKFRPGDVEGEDPGRDRVRRGAFEE 256

Query: 264 AILKIKENTRKLAFRQWQNILRLRSKRGWNLHRIDATKVFQ---HRDNKQHSDHVWEALV 304
           A+  IKENT  LA RQ   ILRL+   GW+L R+DAT+ F+     D+ +    +WE  V
Sbjct: 257 AVRAIKENTCHLAKRQIGKILRLKG-AGWDLRRLDATESFRAAMTSDSGEKCTEIWEKQV 316

[Close]

BLAST of Carg15927 vs. ExPASy Swiss-Prot
Match: Q94ID3 (Adenylate isopentenyltransferase 1, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=IPT1 PE=1 SV=2)

HSP 1 Score: 220.7 bits (561), Expect = 2.4e-56
Identity = 124/296 (41.89%), Postives = 181/296 (61.15%), Query Frame = 0


Query: 35  RKNKLVFVMGATGTGKSRLAVDLATRFPAEIVNSDKIQIYKGLEVVTNKVTEKECCGVPH 94
           RK+K+V ++GATG GKSRL+VDLATRFP+EI+NSDKIQ+Y+GLE+ TN++T ++  GVPH
Sbjct: 63  RKDKVVVILGATGAGKSRLSVDLATRFPSEIINSDKIQVYEGLEITTNQITLQDRRGVPH 122

Query: 95  HLLGIADP-NSNFTAADFRRHATSTINSIVGKHRLPIVAGGSNSFIESLVDGDGEFRY-- 154
           HLLG+ +P +   TA +FR  A++ +  I  + ++PI+AGGSNSF+ +L+    + ++  
Sbjct: 123 HLLGVINPEHGELTAGEFRSAASNVVKEITSRQKVPIIAGGSNSFVHALLAQRFDPKFDP 182

Query: 155 ------------QYECCFLWVDVSLPILHSFVSERVDRMLDNGFINEVRNLFDPNENDY- 214
                       +YECCF+WVDVS  +L+ ++  RVD M+D+G   E+   +DP ++   
Sbjct: 183 FSSGSCLISSDLRYECCFIWVDVSETVLYEYLLRRVDEMMDSGMFEELSRFYDPVKSGLE 242

Query: 215 TR-GIRRAIGVSELDAFLR---AEMDSATDYRARSRILEAAILKIKENTRKLAFRQWQNI 274
           TR GIR+AIGV E D + +    E         R    + A+  IK NT  LA RQ + I
Sbjct: 243 TRFGIRKAIGVPEFDGYFKEYPPEKKMIKWDALRKAAYDKAVDDIKRNTWTLAKRQVKKI 302

Query: 275 LRLRSKRGWNLHRIDATKVFQHRDNKQHSD----HVWEALVTEPASKILHQFLCQN 307
             L+   GW + R+DAT  F+    K  S+      WE  V EP+ KI+ + L QN
Sbjct: 303 EMLKD-AGWEIERVDATASFKAVMMKSSSEKKWRENWEEQVLEPSVKIVKRHLVQN 357

[Close]

BLAST of Carg15927 vs. ExPASy TrEMBL
Match: A0A6J1EWK5 (adenylate isopentenyltransferase 5, chloroplastic-like OS=Cucurbita moschata OX=3662 GN=LOC111436795 PE=4 SV=1)

HSP 1 Score: 639.0 bits (1647), Expect = 1.0e-179
Identity = 315/324 (97.22%), Postives = 319/324 (98.46%), Query Frame = 0


Query: 1   MKISLSASCKKAAAEPPLIDFQGTNTINLKPFFRRKNKLVFVMGATGTGKSRLAVDLATR 60
           MKISLSASCKKAAAEPPLIDFQGTNT+NLKPFFR+K+KLVFVMGATGTGKSRLAVDLATR
Sbjct: 1   MKISLSASCKKAAAEPPLIDFQGTNTVNLKPFFRQKDKLVFVMGATGTGKSRLAVDLATR 60

Query: 61  FPAEIVNSDKIQIYKGLEVVTNKVTEKECCGVPHHLLGIADPNSNFTAADFRRHATSTIN 120
           FPAEIVNSDKIQIYKGLEVVTNKVTEKECCGVPHHLLGIADPNSNFT ADFRRHATSTIN
Sbjct: 61  FPAEIVNSDKIQIYKGLEVVTNKVTEKECCGVPHHLLGIADPNSNFTFADFRRHATSTIN 120

Query: 121 SIVGKHRLPIVAGGSNSFIESLVDGDGEFRYQYECCFLWVDVSLPILHSFVSERVDRMLD 180
           SIVGKHRLPIVAGGSNSFIESLVDGD EFRYQYECCFLWVDVSLPILHSFVSERVDRMLD
Sbjct: 121 SIVGKHRLPIVAGGSNSFIESLVDGDAEFRYQYECCFLWVDVSLPILHSFVSERVDRMLD 180

Query: 181 NGFINEVRNLFDPNENDYTRGIRRAIGVSELDAFLRAEMDSATDYRARSRILEAAILKIK 240
           NGFINEVRNLFDPNENDYTRGIRRAIGV ELDAFLRAEMDSATDYRARSRILEAAILKIK
Sbjct: 181 NGFINEVRNLFDPNENDYTRGIRRAIGVPELDAFLRAEMDSATDYRARSRILEAAILKIK 240

Query: 241 ENTRKLAFRQWQNILRLRSKRGWNLHRIDATKVFQHRDNKQHSDHVWEALVTEPASKILH 300
           ENTRKLAFRQWQNI RLRSKRGWNLHRIDAT+VFQHRDNKQHSDHVWEALVTEPASKILH
Sbjct: 241 ENTRKLAFRQWQNIRRLRSKRGWNLHRIDATEVFQHRDNKQHSDHVWEALVTEPASKILH 300

Query: 301 QFLCQNTYTVNIPTTTIKSNLTVF 325
           QFLCQNTYTVNIPTT IKSNLTVF
Sbjct: 301 QFLCQNTYTVNIPTTAIKSNLTVF 324

[Close]

BLAST of Carg15927 vs. ExPASy TrEMBL
Match: A0A6J1KF69 (adenylate isopentenyltransferase 5, chloroplastic-like OS=Cucurbita maxima OX=3661 GN=LOC111494482 PE=4 SV=1)

HSP 1 Score: 610.9 bits (1574), Expect = 3.0e-171
Identity = 302/324 (93.21%), Postives = 310/324 (95.68%), Query Frame = 0


Query: 1   MKISLSASCKKAAAEPPLIDFQGTNTINLKPFFRRKNKLVFVMGATGTGKSRLAVDLATR 60
           MKISLSASCKKAAAEPPLIDFQ  NTINLKPFFR+K+KLVFVMGATGTGKSRLAV+LATR
Sbjct: 38  MKISLSASCKKAAAEPPLIDFQRRNTINLKPFFRQKDKLVFVMGATGTGKSRLAVNLATR 97

Query: 61  FPAEIVNSDKIQIYKGLEVVTNKVTEKECCGVPHHLLGIADPNSNFTAADFRRHATSTIN 120
           FPAEIVNSDKIQIYKGLEVVTNKVTEKECCGVPHHLLGIADPNSNFTA DFR  A STIN
Sbjct: 98  FPAEIVNSDKIQIYKGLEVVTNKVTEKECCGVPHHLLGIADPNSNFTAVDFRHVAMSTIN 157

Query: 121 SIVGKHRLPIVAGGSNSFIESLVDGDGEFRYQYECCFLWVDVSLPILHSFVSERVDRMLD 180
           SI+G+HRLPIVAGGSNSFIESLVDGD EFRYQYECCFLW+DVSLPILHSFVSERVDRMLD
Sbjct: 158 SIIGRHRLPIVAGGSNSFIESLVDGDAEFRYQYECCFLWIDVSLPILHSFVSERVDRMLD 217

Query: 181 NGFINEVRNLFDPNENDYTRGIRRAIGVSELDAFLRAEMDSATDYRARSRILEAAILKIK 240
           NGFINEVRNLFD NENDYTRGIRRAIGV ELDAFLRAEMDSATDYRARSRILEAAILK K
Sbjct: 218 NGFINEVRNLFDSNENDYTRGIRRAIGVPELDAFLRAEMDSATDYRARSRILEAAILKTK 277

Query: 241 ENTRKLAFRQWQNILRLRSKRGWNLHRIDATKVFQHRDNKQHSDHVWEALVTEPASKILH 300
           ENTRKLAFRQWQNI RLR KRGWNLHRIDAT+VFQHRDN+Q  DHVWEALVTEPASKILH
Sbjct: 278 ENTRKLAFRQWQNICRLRRKRGWNLHRIDATEVFQHRDNEQQFDHVWEALVTEPASKILH 337

Query: 301 QFLCQNTYTVNIPTTTIKSNLTVF 325
           QFLCQNTYTVNIPTTTIKSNLTVF
Sbjct: 338 QFLCQNTYTVNIPTTTIKSNLTVF 361

[Close]

BLAST of Carg15927 vs. ExPASy TrEMBL
Match: A0A6J1H3C2 (adenylate isopentenyltransferase 5, chloroplastic-like OS=Cucurbita moschata OX=3662 GN=LOC111460117 PE=4 SV=1)

HSP 1 Score: 513.5 bits (1321), Expect = 6.6e-142
Identity = 248/307 (80.78%), Postives = 276/307 (89.90%), Query Frame = 0


Query: 1   MKISLSASCKKAAAEPPLIDFQGTNTINLKPFFRRKNKLVFVMGATGTGKSRLAVDLATR 60
           MK SLSASCK+ AAEPPL++ QGTN +NLKPFFR+K+KLVFVMGATGTGKSRLA++LA R
Sbjct: 1   MKFSLSASCKQVAAEPPLVNLQGTNILNLKPFFRQKDKLVFVMGATGTGKSRLAIELANR 60

Query: 61  FPAEIVNSDKIQIYKGLEVVTNKVTEKECCGVPHHLLGIADPNSNFTAADFRRHATSTIN 120
           FPAEI+NSDKIQIYKGLE++TNKVTEKECCGVPHHLLGIADPNSNFTA DFRRHA+  IN
Sbjct: 61  FPAEIINSDKIQIYKGLEILTNKVTEKECCGVPHHLLGIADPNSNFTATDFRRHASLAIN 120

Query: 121 SIVGKHRLPIVAGGSNSFIESLVDGDGEFRYQYECCFLWVDVSLPILHSFVSERVDRMLD 180
           SIVGKHRLPIVAGGSNSFIESLVD + EFRY+Y+CCFLWVDVSLP+LHSFVS+RVDRMLD
Sbjct: 121 SIVGKHRLPIVAGGSNSFIESLVDDEAEFRYRYDCCFLWVDVSLPVLHSFVSDRVDRMLD 180

Query: 181 NGFINEVRNLFDPNENDYTRGIRRAIGVSELDAFLRAEMDSATDYRARSRILEAAILKIK 240
           NG +NEVRNLFDP ENDYTRGIRRAIGV ELDAFL AEMDSATD +ARSRIL AAILKIK
Sbjct: 181 NGLVNEVRNLFDPKENDYTRGIRRAIGVPELDAFLHAEMDSATDDKARSRILNAAILKIK 240

Query: 241 ENTRKLAFRQWQNILRLRSKRGWNLHRIDATKVFQHRDNKQHSDHVWEALVTEPASKILH 300
           EN+ KLA RQ Q I RL+SK GWNLHRIDAT+VF   D  ++SDH WEALV EP+S I+ 
Sbjct: 241 ENSIKLALRQRQKIHRLQSKWGWNLHRIDATEVFLQHDGGEYSDHAWEALVAEPSSIIVD 300

Query: 301 QFLCQNT 308
           QFLC+N+
Sbjct: 301 QFLCENS 307

[Close]

BLAST of Carg15927 vs. ExPASy TrEMBL
Match: A0A6J1KZJ7 (adenylate isopentenyltransferase 5, chloroplastic-like OS=Cucurbita maxima OX=3661 GN=LOC111499016 PE=4 SV=1)

HSP 1 Score: 504.6 bits (1298), Expect = 3.1e-139
Identity = 243/307 (79.15%), Postives = 274/307 (89.25%), Query Frame = 0


Query: 1   MKISLSASCKKAAAEPPLIDFQGTNTINLKPFFRRKNKLVFVMGATGTGKSRLAVDLATR 60
           MK SLSASCK+AA E PL++FQGTN +NLKPFFRRK+KLVFVMGATGTGKSRLA++LA R
Sbjct: 1   MKFSLSASCKQAATESPLVNFQGTNILNLKPFFRRKDKLVFVMGATGTGKSRLAIELANR 60

Query: 61  FPAEIVNSDKIQIYKGLEVVTNKVTEKECCGVPHHLLGIADPNSNFTAADFRRHATSTIN 120
           FPAEI+NSDKIQIYKGLE++TNKVTEKECCGVPHHLLGI DPNSNFTA DF RHA+  IN
Sbjct: 61  FPAEIINSDKIQIYKGLEILTNKVTEKECCGVPHHLLGIVDPNSNFTATDFCRHASLAIN 120

Query: 121 SIVGKHRLPIVAGGSNSFIESLVDGDGEFRYQYECCFLWVDVSLPILHSFVSERVDRMLD 180
           SIVGKHRLPI+AGGSNSFIESLVDG+ EFRY+Y+CCFLWVDVSLP+LHSFVS+RVDRMLD
Sbjct: 121 SIVGKHRLPIIAGGSNSFIESLVDGEAEFRYRYDCCFLWVDVSLPVLHSFVSDRVDRMLD 180

Query: 181 NGFINEVRNLFDPNENDYTRGIRRAIGVSELDAFLRAEMDSATDYRARSRILEAAILKIK 240
           NG +NEVRNLFDP  NDYTRGIRRAIGV ELDAFLRAEMDSATD +ARS+IL+AAILKIK
Sbjct: 181 NGLVNEVRNLFDPKGNDYTRGIRRAIGVPELDAFLRAEMDSATDNKARSQILQAAILKIK 240

Query: 241 ENTRKLAFRQWQNILRLRSKRGWNLHRIDATKVFQHRDNKQHSDHVWEALVTEPASKILH 300
           EN+ KLA RQ Q I  L+SK GWNLHRIDAT+VF      ++S+H WEALV EP+S I+ 
Sbjct: 241 ENSIKLALRQRQKIHHLQSKWGWNLHRIDATEVFLQHGGGEYSEHAWEALVAEPSSIIVD 300

Query: 301 QFLCQNT 308
           QFLC+N+
Sbjct: 301 QFLCENS 307

[Close]

BLAST of Carg15927 vs. ExPASy TrEMBL
Match: A0A0A0KHK3 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_6G237640 PE=4 SV=1)

HSP 1 Score: 470.7 bits (1210), Expect = 4.9e-129
Identity = 242/315 (76.83%), Postives = 278/315 (88.25%), Query Frame = 0


Query: 1   MKISLSASCKKAAAEPPLIDFQGT-NTINLKPFFRRKNKLVFVMGATGTGKSRLAVDLAT 60
           MKISLS SCK+ A EPPL++FQGT NT+NL+PFFRRK+K+VFVMGATGTGKSRLA+DLAT
Sbjct: 1   MKISLSTSCKQIAVEPPLVNFQGTNNTLNLEPFFRRKDKVVFVMGATGTGKSRLAIDLAT 60

Query: 61  RFPAEIVNSDKIQIYKGLEVVTNKVTEKECCGVPHHLLGIADPNSNFTAADFRRHATSTI 120
           RFPAEI+NSDKIQ+YKGL+V+TNKVTEKECCGVPHHLLGIADPNSNFT ADFRRHA+STI
Sbjct: 61  RFPAEIINSDKIQVYKGLKVLTNKVTEKECCGVPHHLLGIADPNSNFTVADFRRHASSTI 120

Query: 121 NSI-VGKHRLPIVAGGSNSFIESLVDGDGEFRYQYECCFLWVDVSLPILHSFVSERVDRM 180
           NSI VGK  LPI+AGGSNSFIESLV+ + EFRY+Y+CCFLWVDVSLPIL+SFVSERVDRM
Sbjct: 121 NSIVVGKRCLPILAGGSNSFIESLVEEEPEFRYRYDCCFLWVDVSLPILNSFVSERVDRM 180

Query: 181 LDNGFINEVRNLFDPN--ENDYTRGIRRAIGVSELDAFLRAEMDSATDYRARSRILEAAI 240
           L+NG +NEVR+LFDPN   NDY  GIRRAIGV ELDAFLRAEMDSATD + RSRIL+AAI
Sbjct: 181 LENGLVNEVRSLFDPNGLGNDYGHGIRRAIGVPELDAFLRAEMDSATDDKTRSRILKAAI 240

Query: 241 LKIKENTRKLAFRQWQNILRLRSK-RGW-NLHRIDATKVFQHRDNKQH-SDHVWEALVTE 300
           LKIKENT KLA RQ Q I RL+SK RGW NLHRIDAT+VF   D++ + SD+ WE LV +
Sbjct: 241 LKIKENTIKLACRQRQKIHRLQSKWRGWNNLHRIDATEVFLQLDSEDYSSDNAWETLVVK 300

Query: 301 PASKILHQFLCQNTY 309
           P+ KI+ QFL +N++
Sbjct: 301 PSFKIVDQFLSENSF 315

[Close]

BLAST of Carg15927 vs. TAIR 10
Match: AT5G19040.1 (isopentenyltransferase 5 )

HSP 1 Score: 336.3 bits (861), Expect = 2.8e-92
Identity = 174/292 (59.59%), Postives = 223/292 (76.37%), Query Frame = 0


Query: 15  EPPLIDFQGTNTINLKPFFRRKNKLVFVMGATGTGKSRLAVDLATRFPAEIVNSDKIQIY 74
           +P  ++FQG N +++ PFFRRK+K+VFVMGATGTGKSRLA+DLATRFPAEIVNSDKIQ+Y
Sbjct: 13  QPLSLNFQG-NMVDV-PFFRRKDKVVFVMGATGTGKSRLAIDLATRFPAEIVNSDKIQVY 72

Query: 75  KGLEVVTNKVTEKECCGVPHHLLG-IADPNSNFTAADFRRHATSTINSIVGKHRLPIVAG 134
           KGL++VTNKVT +E  GVPHHLLG + D   +FTA DF+R A   + SIV + R+PI+AG
Sbjct: 73  KGLDIVTNKVTPEESLGVPHHLLGTVHDTYEDFTAEDFQREAIRAVESIVQRDRVPIIAG 132

Query: 135 GSNSFIESLVDGDGEFRYQYECCFLWVDVSLPILHSFVSERVDRMLDNGFINEVRNLFDP 194
           GSNS+IE+LV+   +FR +Y CCFLWVDVS P+LHSFVSERVD+M+D G ++EVR +FDP
Sbjct: 133 GSNSYIEALVNDCVDFRLRYNCCFLWVDVSRPVLHSFVSERVDKMVDMGLVDEVRRIFDP 192

Query: 195 NENDYTRGIRRAIGVSELDAFLRAEMDSATDYRARS--RILEAAILKIKENTRKLAFRQW 254
           + +DY+ GIRRAIGV ELD FLR+EM    +Y A +  R+LE AI KIKENT  LA RQ 
Sbjct: 193 SSSDYSAGIRRAIGVPELDEFLRSEM---RNYPAETTERLLETAIEKIKENTCLLACRQL 252

Query: 255 QNILRLRSKRGWNLHRIDATKVFQHRDNKQHSDHVWEALVTEPASKILHQFL 304
           Q I RL  +  WN+HR+DAT+VF  R   + +D  W+  V  P++  + +FL
Sbjct: 253 QKIQRLYKQWKWNMHRVDATEVFLRRG--EEADEAWDNSVAHPSALAVEKFL 297

[Close]

BLAST of Carg15927 vs. TAIR 10
Match: AT3G63110.1 (isopentenyltransferase 3 )

HSP 1 Score: 302.8 bits (774), Expect = 3.4e-82
Identity = 151/308 (49.03%), Postives = 216/308 (70.13%), Query Frame = 0


Query: 1   MKISLSASCKKAAAEPPLIDFQ----GTNTINLKPFFRRKNKLVFVMGATGTGKSRLAVD 60
           MKIS+ A CK+     P +DF     G N + L P +  K+K+V +MGATGTGKSRL+VD
Sbjct: 3   MKISM-AMCKQPLPPSPTLDFPPARFGPNMLTLNP-YGPKDKVVVIMGATGTGKSRLSVD 62

Query: 61  LATRFPAEIVNSDKIQIYKGLEVVTNKVTEKECCGVPHHLLGIADPNSNFTAADFRRHAT 120
           +ATRF AEI+NSDKIQ+++GL++VTNK+T +E CGVPHHLLG+  P ++ TAA++   A 
Sbjct: 63  IATRFRAEIINSDKIQVHQGLDIVTNKITSEESCGVPHHLLGVLPPEADLTAANYCHMAN 122

Query: 121 STINSIVGKHRLPIVAGGSNSFIESLVDG-DGEFRYQYECCFLWVDVSLPILHSFVSERV 180
            +I S++ + +LPI+ GGSNS++E+LVD  + +FR +Y+CCFLWVDV+LP+LH FVSERV
Sbjct: 123 LSIESVLNRGKLPIIVGGSNSYVEALVDDKENKFRSRYDCCFLWVDVALPVLHGFVSERV 182

Query: 181 DRMLDNGFINEVRNLFDPNENDYTRGIRRAIGVSELDAFLRAEMDSATDYRARSRILEAA 240
           D+M+++G + EVR  FD + +DY+RGI++AIG  E D F R E     +   R  +L   
Sbjct: 183 DKMVESGMVEEVREFFDFSNSDYSRGIKKAIGFPEFDRFFRNE--QFLNVEDREELLSKV 242

Query: 241 ILKIKENTRKLAFRQWQNILRLRSKRGWNLHRIDATKVFQHRDNKQHSDHVWEALVTEPA 300
           + +IK NT +LA RQ + I RLR  + W++ R+DAT VF  R +K  ++  WE LV  P+
Sbjct: 243 LEEIKRNTFELACRQREKIERLRKVKKWSIQRVDATPVFTKRRSKMDANVAWERLVAGPS 302

Query: 301 SKILHQFL 304
           +  + +FL
Sbjct: 303 TDTVSRFL 306

[Close]

BLAST of Carg15927 vs. TAIR 10
Match: AT3G23630.1 (isopentenyltransferase 7 )

HSP 1 Score: 294.3 bits (752), Expect = 1.2e-79
Identity = 160/313 (51.12%), Postives = 213/313 (68.05%), Query Frame = 0


Query: 1   MKISLSASCKKAAAEPPLIDFQGTNTINLKPFFRRKNKLVFVMGATGTGKSRLAVDLATR 60
           MK S+S S K+   +P L      + +N+  F   K K++FVMGATG+GKSRLA+DLATR
Sbjct: 1   MKFSIS-SLKQ--VQPILCFKNKLSKVNVNSFLHPKEKVIFVMGATGSGKSRLAIDLATR 60

Query: 61  FPAEIVNSDKIQIYKGLEVVTNKVTEKECCGVPHHLLGIADPNS-NFTAADFRRHATSTI 120
           F  EI+NSDKIQ+YKGL+V+TNKVT KEC GVPHHLLG+ D  + N TA  + R A+  I
Sbjct: 61  FQGEIINSDKIQLYKGLDVLTNKVTPKECRGVPHHLLGVFDSEAGNLTATQYSRLASQAI 120

Query: 121 NSIVGKHRLPIVAGGSNSFIESLVD-GDGEFRYQYECCFLWVDVSLPILHSFVSERVDRM 180
           + +   ++LPIVAGGSNS+IE+LV+   G     Y+CCF+WVDVSLP+L+SFVS+RVDRM
Sbjct: 121 SKLSANNKLPIVAGGSNSYIEALVNHSSGFLLNNYDCCFIWVDVSLPVLNSFVSKRVDRM 180

Query: 181 LDNGFINEVRNLFDPNENDYTRGIRRAIGVSELDAFLRAEMDSATDYRARSRILEAAILK 240
           ++ G + EVR +F+P  N Y+ GIRRAIGV EL  +LR E  S  D   +S++L+ A+  
Sbjct: 181 MEAGLLEEVREVFNPKAN-YSVGIRRAIGVPELHEYLRNE--SLVDRATKSKMLDVAVKN 240

Query: 241 IKENTRKLAFRQWQNILRLRSKRGWNLHRIDATKVFQHRDNKQHSDHVWEALVTEPASKI 300
           IK+NT  LA RQ + I RL  K   ++HR+DAT+VF  R N +  D  WE LV  P+ +I
Sbjct: 241 IKKNTEILACRQLKKIQRLHKKWKMSMHRVDATEVFLKR-NVEEQDEAWENLVARPSERI 300

Query: 301 LHQFLCQNTYTVN 312
           + +F   N    N
Sbjct: 301 VDKFYNNNNQLKN 306

[Close]

BLAST of Carg15927 vs. TAIR 10
Match: AT1G68460.1 (isopentenyltransferase 1 )

HSP 1 Score: 220.7 bits (561), Expect = 1.7e-57
Identity = 124/296 (41.89%), Postives = 181/296 (61.15%), Query Frame = 0


Query: 35  RKNKLVFVMGATGTGKSRLAVDLATRFPAEIVNSDKIQIYKGLEVVTNKVTEKECCGVPH 94
           RK+K+V ++GATG GKSRL+VDLATRFP+EI+NSDKIQ+Y+GLE+ TN++T ++  GVPH
Sbjct: 63  RKDKVVVILGATGAGKSRLSVDLATRFPSEIINSDKIQVYEGLEITTNQITLQDRRGVPH 122

Query: 95  HLLGIADP-NSNFTAADFRRHATSTINSIVGKHRLPIVAGGSNSFIESLVDGDGEFRY-- 154
           HLLG+ +P +   TA +FR  A++ +  I  + ++PI+AGGSNSF+ +L+    + ++  
Sbjct: 123 HLLGVINPEHGELTAGEFRSAASNVVKEITSRQKVPIIAGGSNSFVHALLAQRFDPKFDP 182

Query: 155 ------------QYECCFLWVDVSLPILHSFVSERVDRMLDNGFINEVRNLFDPNENDY- 214
                       +YECCF+WVDVS  +L+ ++  RVD M+D+G   E+   +DP ++   
Sbjct: 183 FSSGSCLISSDLRYECCFIWVDVSETVLYEYLLRRVDEMMDSGMFEELSRFYDPVKSGLE 242

Query: 215 TR-GIRRAIGVSELDAFLR---AEMDSATDYRARSRILEAAILKIKENTRKLAFRQWQNI 274
           TR GIR+AIGV E D + +    E         R    + A+  IK NT  LA RQ + I
Sbjct: 243 TRFGIRKAIGVPEFDGYFKEYPPEKKMIKWDALRKAAYDKAVDDIKRNTWTLAKRQVKKI 302

Query: 275 LRLRSKRGWNLHRIDATKVFQHRDNKQHSD----HVWEALVTEPASKILHQFLCQN 307
             L+   GW + R+DAT  F+    K  S+      WE  V EP+ KI+ + L QN
Sbjct: 303 EMLKD-AGWEIERVDATASFKAVMMKSSSEKKWRENWEEQVLEPSVKIVKRHLVQN 357

[Close]

BLAST of Carg15927 vs. TAIR 10
Match: AT3G19160.1 (ATP/ADP isopentenyltransferases )

HSP 1 Score: 214.9 bits (546), Expect = 9.4e-56
Identity = 123/294 (41.84%), Postives = 181/294 (61.56%), Query Frame = 0


Query: 36  KNKLVFVMGATGTGKSRLAVDLATRFPAEIVNSDKIQIYKGLEVVTNKVTEKECCGVPHH 95
           K K+V +MGATG+GKS L++DLATRF  EIVNSDKIQ Y GL+V TN+++  E CGVPHH
Sbjct: 42  KQKVVVIMGATGSGKSCLSIDLATRFSGEIVNSDKIQFYDGLKVTTNQMSILERCGVPHH 101

Query: 96  LLGIADP-NSNFTAADFRRHATSTINSIVGKHRLPIVAGGSNSFIES-LVDGDGEFRY-- 155
           LLG   P +S  T ++FR  A+ +I+ I  +  LPI+AGGSNSFI + LVD      Y  
Sbjct: 102 LLGELPPDDSELTTSEFRSLASRSISEITARGNLPIIAGGSNSFIHALLVDRFDPKTYPF 161

Query: 156 ----------QYECCFLWVDVSLPILHSFVSERVDRMLDNGFINEVRNLFDPNEND---Y 215
                     +YECCFLWVDVS+ +L  ++S+RVD+M+++G   E+   +DP  +     
Sbjct: 162 SSETSISSGLRYECCFLWVDVSVSVLFEYLSKRVDQMMESGMFEELAGFYDPRYSGSAIR 221

Query: 216 TRGIRRAIGVSELDAFL------RAEMDSATDYRARSRILEAAILKIKENTRKLAFRQWQ 275
             GI + IG+ E D +       R +  S  D +AR    + A+ +IKENT +LA +Q +
Sbjct: 222 AHGIHKTIGIPEFDRYFSLYPPERKQKMSEWD-QARKGAYDEAVQEIKENTWRLAKKQIE 281

Query: 276 NILRLRSKRGWNLHRIDATKVFQHRDNKQHSDHVWEALVTEPASKILHQFLCQN 307
            I++L+S  GW++ R+DAT  F      + S  +W+  V + + K++ +FL ++
Sbjct: 282 RIMKLKSS-GWDIQRLDATPSF-----GRSSREIWDNTVLDESIKVVKRFLVKD 328

The following BLAST results are available for this feature:

BLAST of Carg15927 vs. NCBI nr


Match Name	E-value	Identity	Description
KAG7026259.1	2.9e-184	100.00	Adenylate isopentenyltransferase 5, chloroplastic, partial [Cucurbita argyrosper...	[more]
KAG6593917.1	6.7e-181	98.46	Adenylate isopentenyltransferase 5, chloroplastic, partial [Cucurbita argyrosper...	[more]
XP_023522124.1	4.4e-180	97.84	adenylate isopentenyltransferase 5, chloroplastic-like [Cucurbita pepo subsp. pe...	[more]
XP_022930300.1	2.2e-179	97.22	adenylate isopentenyltransferase 5, chloroplastic-like [Cucurbita moschata]	[more]
XP_023513929.1	3.1e-178	97.22	adenylate isopentenyltransferase 5, chloroplastic-like [Cucurbita pepo subsp. pe...	[more]

BLAST of Carg15927 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q94ID2	3.9e-91	59.59	Adenylate isopentenyltransferase 5, chloroplastic OS=Arabidopsis thaliana OX=370...	[more]
Q93WC9	4.8e-81	49.03	Adenylate isopentenyltransferase 3, chloroplastic OS=Arabidopsis thaliana OX=370...	[more]
Q94ID1	1.7e-78	51.12	Adenylate isopentenyltransferase 7, mitochondrial OS=Arabidopsis thaliana OX=370...	[more]
Q5GHF7	3.6e-60	42.31	Adenylate isopentenyltransferase OS=Humulus lupulus OX=3486 PE=1 SV=1	[more]
Q94ID3	2.4e-56	41.89	Adenylate isopentenyltransferase 1, chloroplastic OS=Arabidopsis thaliana OX=370...	[more]

BLAST of Carg15927 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1EWK5	1.0e-179	97.22	adenylate isopentenyltransferase 5, chloroplastic-like OS=Cucurbita moschata OX=...	[more]
A0A6J1KF69	3.0e-171	93.21	adenylate isopentenyltransferase 5, chloroplastic-like OS=Cucurbita maxima OX=36...	[more]
A0A6J1H3C2	6.6e-142	80.78	adenylate isopentenyltransferase 5, chloroplastic-like OS=Cucurbita moschata OX=...	[more]
A0A6J1KZJ7	3.1e-139	79.15	adenylate isopentenyltransferase 5, chloroplastic-like OS=Cucurbita maxima OX=36...	[more]
A0A0A0KHK3	4.9e-129	76.83	Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_6G237640 PE=4 SV=1	[more]

BLAST of Carg15927 vs. TAIR 10


Match Name	E-value	Identity	Description
AT5G19040.1	2.8e-92	59.59	isopentenyltransferase 5	[more]
AT3G63110.1	3.4e-82	49.03	isopentenyltransferase 3	[more]
AT3G23630.1	1.2e-79	51.12	isopentenyltransferase 7	[more]
AT1G68460.1	1.7e-57	41.89	isopentenyltransferase 1	[more]
AT3G19160.1	9.4e-56	41.84	ATP/ADP isopentenyltransferases	[more]

InterPro

Analysis Name: InterPro Annotations of Silver-seed gourd (SMH-JMG-627) v2
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
None	No IPR available	PFAM	PF01715	IPPT	coord: 151..252 e-value: 6.0E-12 score: 45.8 coord: 72..145 e-value: 4.7E-21 score: 75.6
None	No IPR available	GENE3D	1.10.287.890	Crystal structure of tRNA isopentenylpyrophosphate transferase (bh2366) domain	coord: 164..265 e-value: 1.9E-104 score: 350.6
None	No IPR available	PANTHER	PTHR11088:SF74	ADENYLATE ISOPENTENYLTRANSFERASE 5, CHLOROPLASTIC	coord: 27..308
IPR027417	P-loop containing nucleoside triphosphate hydrolase	GENE3D	3.40.50.300		coord: 36..303 e-value: 1.9E-104 score: 350.6
IPR027417	P-loop containing nucleoside triphosphate hydrolase	SUPERFAMILY	52540	P-loop containing nucleoside triphosphate hydrolases	coord: 39..148
IPR039657	Dimethylallyltransferase	PANTHER	PTHR11088	TRNA DIMETHYLALLYLTRANSFERASE	coord: 27..308

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Carg15927-RA	Carg15927-RA	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0009691	cytokinin biosynthetic process
biological_process	GO:0006400	tRNA modification
cellular_component	GO:0005739	mitochondrion
molecular_function	GO:0009824	AMP dimethylallyltransferase activity
molecular_function	GO:0005524	ATP binding
molecular_function	GO:0052381	tRNA dimethylallyltransferase activity