Carg15878 (gene) Silver-seed gourd (SMH-JMG-627) v2

Overview
NameCarg15878
Typegene
OrganismCucurbita argyrosperma subsp. argyrosperma cv. SMH-JMG-627 (Silver-seed gourd (SMH-JMG-627) v2)
DescriptionPyruvate kinase
LocationCarg_Chr08: 6879169 .. 6883248 (-)
RNA-Seq ExpressionCarg15878
SyntenyCarg15878
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideexonCDSthree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
TTCAATTACAGTGCGAAATCTGTAACACGTTTTCCGCCGTCGATCATCCGGCGGCAATTCCGAAGCTATCAACACCAAAATAATTCGTATTCCTTTGGAATCTCCGTACCTCTCTCTGCTTTAATCACCATCTCCATTTTCTCACTTCCCATTTCCTTCTCCAAATTCGAAATTCCCCTCACAATGGCACAATCTCTGCAACTTTTCACCTCCTCCACCACCCGCTCTCCGCCCATTTCTCTCCCCAAACATTCCCTCTCAAAAACCCCCTCCTCTTCCACCGCCGCCTTCCGCCTCCCTGTCTCCTTCAACAAATTTTCAATTCGAGCCTCTTCCTCTCCGGATCTCAACCCTCTGTCATCAGCTTCCACTTCCCAAGTCTTGGTTTCTGAAAATGGTTCGAGTTCCGGAGGTGGGTTTGTGTCTACTTCTGCTACTACCAGGGAGTTTGTGCCGGCTGTGTCTGATGGCAGCTCGATTGACGTCGATACTGTCACGGAGGCTGAGTTGAAGGAGAATGGCTTCAGGAGTACAAGGAGGACGAAGCTTGTGTGTACTATTGGTCCTGTTACTTGCGGATTTGAGCAGCTTGAAGCGCTTGCTGTTGGCGGTATGAATGTTGCGAGGATCAATATGTGCCATGGAACTCGAGAGTGGCATCGGATGGTCATTGAACGCGTGCGCAGGCTCAATGAGGAGAAGGGCTATGCTGTGGCTATTATGATGGATACTGAAGGGAGTGAAATTCATATGGGTGATCTTGGTGGAGCTTCCTCGGCTAAAGCGGAAGTGAGTTCTGTTCTGTTCTTTATCTTTTTCCATCTTATTGAATCACAACCGGTCGAATCTTTGCTAATATGTGTCCTGGATTTAAAAACTTCACTGGGTTTTGTCTGGCTCCCAATTGGAGGACATTGTTAAGTTCTTGATTTAGAAGCAGAAAAACATTTGGATTTCAAATTAGATGGGTCCTTCATCATTAAGCGAGTTGTGTACTTGTGAAAGGGCTTTTAAGGACATGTATAACTGCTTGCTCTGTTCTATATAGGATGGTGAAATCTGGACATTCAGTGTCAGAGCTTTTGATTCAACACTCCCAGAACGCACCATTAATGTGAACTACGAAGGGTTTGCAGAAGGTTACTGATTCTATCCTTAGATCAAAATTTTGCTCTGACATTTACTGAATTCATTCACAATCAACTATACATATAGTATTTGGGCATGCCATATGTGGATCGGATGTCTGTGTTCATTATTATAGTAAATTCCATGGTGTGTTATCAATCTTGAATTGTAATTGATATTGTGTAGATGTGAGAGTTGGTGATGATCTCCTTGTTGATGGTGGAATGGTGAGGTTTGAAGTAATCGAAAAGATTGGTCCTGATGTTAAGTGCCTGTGTACTGACCCGGGGTTGTTGTTGCCACGGGCTAATTTGACTTTTTGGAGGGATGGACATCTCGTACGAGAACGTAACGCCATGCTCCCTACAATTTCTTCTAAGGTAGGTGCCTCCCTTTTCCTTAAACTTCATTTGTTTTAGGGTCACTTTTAAAGAATTAATACAATATTAAATTTATCATAATCTATCAACTTTCGGATCAATGGTATCTTAACATAGTATCAGAGTAGAAGTTCATGTTCAAATCTCTATAATGTCATTTCCTCCCCAATTAAGATTGATAACTATTTGTTGTGTCTCGTGCAAATTTCTAACCCCACAAGTGAAGGGCGTTAAAGTATTGGATATAATATAAAATTTACTCTTAGTTACTCGTAACTAGATTTAAGTTGAAAGCTAATGTAGAGAAAATTATGATTTGCACCATAAATTTTAGGAGGACATGTTTAATGCGGTCAACGGTAATAATATCGTACCCTAAACTCTTTATGCAACCGAATGATTGCATCTGTATGTGGATCATAGTCTCATTAGATTTGATGGCTAACCTTTTGTGCAACCTAATGGCGTAACTTGCCCTGATACAGTGACTTGTTTTCCAGTCTAAGGCAAGTTTTATGAATCCAGGATTGGTTGGATATTGATTTTGGGATTTCTGAAGGTGTTGATTTTCTTGCCATATCATTTGTCAAGTCTGCTGAAGTGATTAAACATCTCAAAAGCTATATTGCTGCACGTTCTCGTGGCAGGTAAGCAGAATTGTGCGAGCTTGCTGATTTAATCTTCTTCTTTACCATTTGGTTTTGAAGCTGTGGGTGTAATTGTTGGTTCTCACGGTTTTAGGGGGATCAAATATATCTCAACTATGTTTTAGTATATCACTGATTCACAAGTTAGGTGTGAGATCCCACATCAGTTGGAGAGGAGAACGAAACATTTTTTATAAGGGTGTAGAAACCTCTCCATATTAGACGCGTTTTAAAAACCTTAAGGGAAACCTGAAAGGGAAAACCCTAAGAGGACAATATCTGCTAGCGGTGGATTTGGGCTGTTACATTAGGTGCAGATGGGGTAGTCGACGACCACGTTTTCACTCTAAATCTGAATTTCATCTCTATCTCTAAATGATATGTGCGCCTTCGAAATATCTGTGTTTATAAATTTGTTATGCAGTGACATTTCCATCATTGCGAAGATAGAGAGTCTGGACTCATTGAAGAACTTGGAAGAAATTATTGTAGCATCTGATGGAGCTATGGTAGCCAGAGGAGATTTAGGAGCTCAAATACCACTGGAACAGGTGCCATCAGTCCAGCAAAAGGTCGTCCAACTATGCAGACGGTTAAATAAGCCAGTTATTGTTGCCTCTCAGCTGCTCGAGTCGATGATCGAATACCCTACACCTACCAGAGCTGAAGTCGCTGATGTTTCCGAGGCTGTTAGGCAGCGAGCAGATGCTCTAATGCTCTCTGGTGAGTCCGCCATGGGCCAGTTCCCCGAGAAGGCATTGACCGTCTTGAGAAGCGTCAGTCTAAGAATTGAGAAGTGGTGGAGGGATGAGAAACGCCATGAAGCTATGGAACTCCCAGAAGTTGGATCTTCATACTCGGATAGCATCTTAGAAGAGATCTGCAATTCCACTGCCAAAATGGGTAAGCTTTATCATATTCTCCAGCTATGGATCATATACCTATATTATAGTTTAGATCTATGCTTTAGTACTAAGAACTCGAATACTATCTCAAATCGTTTTTACAAAAGTGATCGTTATGCTGTGTTACAACGCCAATATTGAATCACCATGTATTGTTTAGAGGGAATCAAATGTAGTAGGGTCAGGTGGTTATCTCGAGAGAGTAGTCGAAGTGTATACAAACTGGATCGAACACTCACAGACATAAAAAAAAAATGCGAGCGTTGAAGATGGTTGGTTGAGTCGGGCGTCGTCATACGCTACTATCTTCTTTCCCTCAGTTCTTTATTGCACTTCTCCTGTTGGTTTGGTTATTCCAGTCTAATTGTGAGCTTTGTTTCTGTAGCCAATAATTTGGAGGTGGATGCAATTTTTGTCTACACAAAGACAGGCCACATGGCATCTCTCCTATCCCGTTGCAGACCCGATTGCCCGATCTTTGCGTTTACTTCCACGACATCTGTGAGGAGACGTCTTAACTTGCAATGGGGCTTGATACCCTTCCGCTTGAGCTTCTCGGACGACATGGAAAACAACCTCAACAAAACGTTTATGCTGCTTAAAGCCAGAAACTTGATAAAATCAGGTGACCTTGTACTTGCTGTATCAGACATGCTGCAGTCTATTCAAGTAATGAATGTTCCATAAGAAAAAGAGGGGTTCTGTAATGTGCTTGCTTGCTCTGATGTTTCATTCCCCCAAAACTATTTGACTTAATCTAAGCCTCTGGATCTGTTACTTAGCTGCTAGGATAAGAGATCGACTCGACTCGAGCTTCAGGTTTTTCTCCTTCGACCCTATCTGAAATGTTTAGTTGAACTTTTGAGCATTTTGGTTTATTTCTTCTTATTCTCTGTAGTGTTTTTATGGGTATCAGATAAATGTTCTATGAACAAACATGGTCTGCGTGCTATTTATCTAATTTTTTTTAGATATTAATACGTAATATTATGTATTTCGGCGTTTGATTGATAAAAAAAG

mRNA sequence

TTCAATTACAGTGCGAAATCTGTAACACGTTTTCCGCCGTCGATCATCCGGCGGCAATTCCGAAGCTATCAACACCAAAATAATTCGTATTCCTTTGGAATCTCCGTACCTCTCTCTGCTTTAATCACCATCTCCATTTTCTCACTTCCCATTTCCTTCTCCAAATTCGAAATTCCCCTCACAATGGCACAATCTCTGCAACTTTTCACCTCCTCCACCACCCGCTCTCCGCCCATTTCTCTCCCCAAACATTCCCTCTCAAAAACCCCCTCCTCTTCCACCGCCGCCTTCCGCCTCCCTGTCTCCTTCAACAAATTTTCAATTCGAGCCTCTTCCTCTCCGGATCTCAACCCTCTGTCATCAGCTTCCACTTCCCAAGTCTTGGTTTCTGAAAATGGTTCGAGTTCCGGAGGTGGGTTTGTGTCTACTTCTGCTACTACCAGGGAGTTTGTGCCGGCTGTGTCTGATGGCAGCTCGATTGACGTCGATACTGTCACGGAGGCTGAGTTGAAGGAGAATGGCTTCAGGAGTACAAGGAGGACGAAGCTTGTGTGTACTATTGGTCCTGTTACTTGCGGATTTGAGCAGCTTGAAGCGCTTGCTGTTGGCGGTATGAATGTTGCGAGGATCAATATGTGCCATGGAACTCGAGAGTGGCATCGGATGGTCATTGAACGCGTGCGCAGGCTCAATGAGGAGAAGGGCTATGCTGTGGCTATTATGATGGATACTGAAGGGAGTGAAATTCATATGGGTGATCTTGGTGGAGCTTCCTCGGCTAAAGCGGAAGATGGTGAAATCTGGACATTCAGTGTCAGAGCTTTTGATTCAACACTCCCAGAACGCACCATTAATGTGAACTACGAAGGGTTTGCAGAAGATGTGAGAGTTGGTGATGATCTCCTTGTTGATGGTGGAATGGTGAGGTTTGAAGTAATCGAAAAGATTGGTCCTGATGTTAAGTGCCTGTGTACTGACCCGGGGTTGTTGTTGCCACGGGCTAATTTGACTTTTTGGAGGGATGGACATCTCGTACGAGAACGTAACGCCATGCTCCCTACAATTTCTTCTAAGGATTGGTTGGATATTGATTTTGGGATTTCTGAAGGTGTTGATTTTCTTGCCATATCATTTGTCAAGTCTGCTGAAGTGATTAAACATCTCAAAAGCTATATTGCTGCACGTTCTCGTGGCAGTGACATTTCCATCATTGCGAAGATAGAGAGTCTGGACTCATTGAAGAACTTGGAAGAAATTATTGTAGCATCTGATGGAGCTATGGTAGCCAGAGGAGATTTAGGAGCTCAAATACCACTGGAACAGGTGCCATCAGTCCAGCAAAAGGTCGTCCAACTATGCAGACGGTTAAATAAGCCAGTTATTGTTGCCTCTCAGCTGCTCGAGTCGATGATCGAATACCCTACACCTACCAGAGCTGAAGTCGCTGATGTTTCCGAGGCTGTTAGGCAGCGAGCAGATGCTCTAATGCTCTCTGGTGAGTCCGCCATGGGCCAGTTCCCCGAGAAGGCATTGACCGTCTTGAGAAGCGTCAGTCTAAGAATTGAGAAGTGGTGGAGGGATGAGAAACGCCATGAAGCTATGGAACTCCCAGAAGTTGGATCTTCATACTCGGATAGCATCTTAGAAGAGATCTGCAATTCCACTGCCAAAATGGCCAATAATTTGGAGGTGGATGCAATTTTTGTCTACACAAAGACAGGCCACATGGCATCTCTCCTATCCCGTTGCAGACCCGATTGCCCGATCTTTGCGTTTACTTCCACGACATCTGTGAGGAGACGTCTTAACTTGCAATGGGGCTTGATACCCTTCCGCTTGAGCTTCTCGGACGACATGGAAAACAACCTCAACAAAACGTTTATGCTGCTTAAAGCCAGAAACTTGATAAAATCAGGTGACCTTGTACTTGCTGTATCAGACATGCTGCAGTCTATTCAAGTAATGAATGTTCCATAAGAAAAAGAGGGGTTCTGTAATGTGCTTGCTTGCTCTGATGTTTCATTCCCCCAAAACTATTTGACTTAATCTAAGCCTCTGGATCTGTTACTTAGCTGCTAGGATAAGAGATCGACTCGACTCGAGCTTCAGGTTTTTCTCCTTCGACCCTATCTGAAATGTTTAGTTGAACTTTTGAGCATTTTGGTTTATTTCTTCTTATTCTCTGTAGTGTTTTTATGGGTATCAGATAAATGTTCTATGAACAAACATGGTCTGCGTGCTATTTATCTAATTTTTTTTAGATATTAATACGTAATATTATGTATTTCGGCGTTTGATTGATAAAAAAAG

Coding sequence (CDS)

TTCAATTACAGTGCGAAATCTGTAACACGTTTTCCGCCGTCGATCATCCGGCGGCAATTCCGAAGCTATCAACACCAAAATAATTCGTATTCCTTTGGAATCTCCGTACCTCTCTCTGCTTTAATCACCATCTCCATTTTCTCACTTCCCATTTCCTTCTCCAAATTCGAAATTCCCCTCACAATGGCACAATCTCTGCAACTTTTCACCTCCTCCACCACCCGCTCTCCGCCCATTTCTCTCCCCAAACATTCCCTCTCAAAAACCCCCTCCTCTTCCACCGCCGCCTTCCGCCTCCCTGTCTCCTTCAACAAATTTTCAATTCGAGCCTCTTCCTCTCCGGATCTCAACCCTCTGTCATCAGCTTCCACTTCCCAAGTCTTGGTTTCTGAAAATGGTTCGAGTTCCGGAGGTGGGTTTGTGTCTACTTCTGCTACTACCAGGGAGTTTGTGCCGGCTGTGTCTGATGGCAGCTCGATTGACGTCGATACTGTCACGGAGGCTGAGTTGAAGGAGAATGGCTTCAGGAGTACAAGGAGGACGAAGCTTGTGTGTACTATTGGTCCTGTTACTTGCGGATTTGAGCAGCTTGAAGCGCTTGCTGTTGGCGGTATGAATGTTGCGAGGATCAATATGTGCCATGGAACTCGAGAGTGGCATCGGATGGTCATTGAACGCGTGCGCAGGCTCAATGAGGAGAAGGGCTATGCTGTGGCTATTATGATGGATACTGAAGGGAGTGAAATTCATATGGGTGATCTTGGTGGAGCTTCCTCGGCTAAAGCGGAAGATGGTGAAATCTGGACATTCAGTGTCAGAGCTTTTGATTCAACACTCCCAGAACGCACCATTAATGTGAACTACGAAGGGTTTGCAGAAGATGTGAGAGTTGGTGATGATCTCCTTGTTGATGGTGGAATGGTGAGGTTTGAAGTAATCGAAAAGATTGGTCCTGATGTTAAGTGCCTGTGTACTGACCCGGGGTTGTTGTTGCCACGGGCTAATTTGACTTTTTGGAGGGATGGACATCTCGTACGAGAACGTAACGCCATGCTCCCTACAATTTCTTCTAAGGATTGGTTGGATATTGATTTTGGGATTTCTGAAGGTGTTGATTTTCTTGCCATATCATTTGTCAAGTCTGCTGAAGTGATTAAACATCTCAAAAGCTATATTGCTGCACGTTCTCGTGGCAGTGACATTTCCATCATTGCGAAGATAGAGAGTCTGGACTCATTGAAGAACTTGGAAGAAATTATTGTAGCATCTGATGGAGCTATGGTAGCCAGAGGAGATTTAGGAGCTCAAATACCACTGGAACAGGTGCCATCAGTCCAGCAAAAGGTCGTCCAACTATGCAGACGGTTAAATAAGCCAGTTATTGTTGCCTCTCAGCTGCTCGAGTCGATGATCGAATACCCTACACCTACCAGAGCTGAAGTCGCTGATGTTTCCGAGGCTGTTAGGCAGCGAGCAGATGCTCTAATGCTCTCTGGTGAGTCCGCCATGGGCCAGTTCCCCGAGAAGGCATTGACCGTCTTGAGAAGCGTCAGTCTAAGAATTGAGAAGTGGTGGAGGGATGAGAAACGCCATGAAGCTATGGAACTCCCAGAAGTTGGATCTTCATACTCGGATAGCATCTTAGAAGAGATCTGCAATTCCACTGCCAAAATGGCCAATAATTTGGAGGTGGATGCAATTTTTGTCTACACAAAGACAGGCCACATGGCATCTCTCCTATCCCGTTGCAGACCCGATTGCCCGATCTTTGCGTTTACTTCCACGACATCTGTGAGGAGACGTCTTAACTTGCAATGGGGCTTGATACCCTTCCGCTTGAGCTTCTCGGACGACATGGAAAACAACCTCAACAAAACGTTTATGCTGCTTAAAGCCAGAAACTTGATAAAATCAGGTGACCTTGTACTTGCTGTATCAGACATGCTGCAGTCTATTCAAGTAATGAATGTTCCATAA

Protein sequence

FNYSAKSVTRFPPSIIRRQFRSYQHQNNSYSFGISVPLSALITISIFSLPISFSKFEIPLTMAQSLQLFTSSTTRSPPISLPKHSLSKTPSSSTAAFRLPVSFNKFSIRASSSPDLNPLSSASTSQVLVSENGSSSGGGFVSTSATTREFVPAVSDGSSIDVDTVTEAELKENGFRSTRRTKLVCTIGPVTCGFEQLEALAVGGMNVARINMCHGTREWHRMVIERVRRLNEEKGYAVAIMMDTEGSEIHMGDLGGASSAKAEDGEIWTFSVRAFDSTLPERTINVNYEGFAEDVRVGDDLLVDGGMVRFEVIEKIGPDVKCLCTDPGLLLPRANLTFWRDGHLVRERNAMLPTISSKDWLDIDFGISEGVDFLAISFVKSAEVIKHLKSYIAARSRGSDISIIAKIESLDSLKNLEEIIVASDGAMVARGDLGAQIPLEQVPSVQQKVVQLCRRLNKPVIVASQLLESMIEYPTPTRAEVADVSEAVRQRADALMLSGESAMGQFPEKALTVLRSVSLRIEKWWRDEKRHEAMELPEVGSSYSDSILEEICNSTAKMANNLEVDAIFVYTKTGHMASLLSRCRPDCPIFAFTSTTSVRRRLNLQWGLIPFRLSFSDDMENNLNKTFMLLKARNLIKSGDLVLAVSDMLQSIQVMNVP
Homology
BLAST of Carg15878 vs. NCBI nr
Match: KAG7026211.1 (hypothetical protein SDJN02_12710, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 1265.8 bits (3274), Expect = 0.0e+00
Identity = 658/658 (100.00%), Postives = 658/658 (100.00%), Query Frame = 0

Query: 1   FNYSAKSVTRFPPSIIRRQFRSYQHQNNSYSFGISVPLSALITISIFSLPISFSKFEIPL 60
           FNYSAKSVTRFPPSIIRRQFRSYQHQNNSYSFGISVPLSALITISIFSLPISFSKFEIPL
Sbjct: 1   FNYSAKSVTRFPPSIIRRQFRSYQHQNNSYSFGISVPLSALITISIFSLPISFSKFEIPL 60

Query: 61  TMAQSLQLFTSSTTRSPPISLPKHSLSKTPSSSTAAFRLPVSFNKFSIRASSSPDLNPLS 120
           TMAQSLQLFTSSTTRSPPISLPKHSLSKTPSSSTAAFRLPVSFNKFSIRASSSPDLNPLS
Sbjct: 61  TMAQSLQLFTSSTTRSPPISLPKHSLSKTPSSSTAAFRLPVSFNKFSIRASSSPDLNPLS 120

Query: 121 SASTSQVLVSENGSSSGGGFVSTSATTREFVPAVSDGSSIDVDTVTEAELKENGFRSTRR 180
           SASTSQVLVSENGSSSGGGFVSTSATTREFVPAVSDGSSIDVDTVTEAELKENGFRSTRR
Sbjct: 121 SASTSQVLVSENGSSSGGGFVSTSATTREFVPAVSDGSSIDVDTVTEAELKENGFRSTRR 180

Query: 181 TKLVCTIGPVTCGFEQLEALAVGGMNVARINMCHGTREWHRMVIERVRRLNEEKGYAVAI 240
           TKLVCTIGPVTCGFEQLEALAVGGMNVARINMCHGTREWHRMVIERVRRLNEEKGYAVAI
Sbjct: 181 TKLVCTIGPVTCGFEQLEALAVGGMNVARINMCHGTREWHRMVIERVRRLNEEKGYAVAI 240

Query: 241 MMDTEGSEIHMGDLGGASSAKAEDGEIWTFSVRAFDSTLPERTINVNYEGFAEDVRVGDD 300
           MMDTEGSEIHMGDLGGASSAKAEDGEIWTFSVRAFDSTLPERTINVNYEGFAEDVRVGDD
Sbjct: 241 MMDTEGSEIHMGDLGGASSAKAEDGEIWTFSVRAFDSTLPERTINVNYEGFAEDVRVGDD 300

Query: 301 LLVDGGMVRFEVIEKIGPDVKCLCTDPGLLLPRANLTFWRDGHLVRERNAMLPTISSKDW 360
           LLVDGGMVRFEVIEKIGPDVKCLCTDPGLLLPRANLTFWRDGHLVRERNAMLPTISSKDW
Sbjct: 301 LLVDGGMVRFEVIEKIGPDVKCLCTDPGLLLPRANLTFWRDGHLVRERNAMLPTISSKDW 360

Query: 361 LDIDFGISEGVDFLAISFVKSAEVIKHLKSYIAARSRGSDISIIAKIESLDSLKNLEEII 420
           LDIDFGISEGVDFLAISFVKSAEVIKHLKSYIAARSRGSDISIIAKIESLDSLKNLEEII
Sbjct: 361 LDIDFGISEGVDFLAISFVKSAEVIKHLKSYIAARSRGSDISIIAKIESLDSLKNLEEII 420

Query: 421 VASDGAMVARGDLGAQIPLEQVPSVQQKVVQLCRRLNKPVIVASQLLESMIEYPTPTRAE 480
           VASDGAMVARGDLGAQIPLEQVPSVQQKVVQLCRRLNKPVIVASQLLESMIEYPTPTRAE
Sbjct: 421 VASDGAMVARGDLGAQIPLEQVPSVQQKVVQLCRRLNKPVIVASQLLESMIEYPTPTRAE 480

Query: 481 VADVSEAVRQRADALMLSGESAMGQFPEKALTVLRSVSLRIEKWWRDEKRHEAMELPEVG 540
           VADVSEAVRQRADALMLSGESAMGQFPEKALTVLRSVSLRIEKWWRDEKRHEAMELPEVG
Sbjct: 481 VADVSEAVRQRADALMLSGESAMGQFPEKALTVLRSVSLRIEKWWRDEKRHEAMELPEVG 540

Query: 541 SSYSDSILEEICNSTAKMANNLEVDAIFVYTKTGHMASLLSRCRPDCPIFAFTSTTSVRR 600
           SSYSDSILEEICNSTAKMANNLEVDAIFVYTKTGHMASLLSRCRPDCPIFAFTSTTSVRR
Sbjct: 541 SSYSDSILEEICNSTAKMANNLEVDAIFVYTKTGHMASLLSRCRPDCPIFAFTSTTSVRR 600

Query: 601 RLNLQWGLIPFRLSFSDDMENNLNKTFMLLKARNLIKSGDLVLAVSDMLQSIQVMNVP 659
           RLNLQWGLIPFRLSFSDDMENNLNKTFMLLKARNLIKSGDLVLAVSDMLQSIQVMNVP
Sbjct: 601 RLNLQWGLIPFRLSFSDDMENNLNKTFMLLKARNLIKSGDLVLAVSDMLQSIQVMNVP 658

BLAST of Carg15878 vs. NCBI nr
Match: KAG6593869.1 (hypothetical protein SDJN03_13345, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 1257.3 bits (3252), Expect = 0.0e+00
Identity = 654/655 (99.85%), Postives = 654/655 (99.85%), Query Frame = 0

Query: 4   SAKSVTRFPPSIIRRQFRSYQHQNNSYSFGISVPLSALITISIFSLPISFSKFEIPLTMA 63
           S KSVTRFPPSIIRRQFRSYQHQNNSYSFGISVPLSALITISIFSLPISFSKFEIPLTMA
Sbjct: 112 SGKSVTRFPPSIIRRQFRSYQHQNNSYSFGISVPLSALITISIFSLPISFSKFEIPLTMA 171

Query: 64  QSLQLFTSSTTRSPPISLPKHSLSKTPSSSTAAFRLPVSFNKFSIRASSSPDLNPLSSAS 123
           QSLQLFTSSTTRSPPISLPKHSLSKTPSSSTAAFRLPVSFNKFSIRASSSPDLNPLSSAS
Sbjct: 172 QSLQLFTSSTTRSPPISLPKHSLSKTPSSSTAAFRLPVSFNKFSIRASSSPDLNPLSSAS 231

Query: 124 TSQVLVSENGSSSGGGFVSTSATTREFVPAVSDGSSIDVDTVTEAELKENGFRSTRRTKL 183
           TSQVLVSENGSSSGGGFVSTSATTREFVPAVSDGSSIDVDTVTEAELKENGFRSTRRTKL
Sbjct: 232 TSQVLVSENGSSSGGGFVSTSATTREFVPAVSDGSSIDVDTVTEAELKENGFRSTRRTKL 291

Query: 184 VCTIGPVTCGFEQLEALAVGGMNVARINMCHGTREWHRMVIERVRRLNEEKGYAVAIMMD 243
           VCTIGPVTCGFEQLEALAVGGMNVARINMCHGTREWHRMVIERVRRLNEEKGYAVAIMMD
Sbjct: 292 VCTIGPVTCGFEQLEALAVGGMNVARINMCHGTREWHRMVIERVRRLNEEKGYAVAIMMD 351

Query: 244 TEGSEIHMGDLGGASSAKAEDGEIWTFSVRAFDSTLPERTINVNYEGFAEDVRVGDDLLV 303
           TEGSEIHMGDLGGASSAKAEDGEIWTFSVRAFDSTLPERTINVNYEGFAEDVRVGDDLLV
Sbjct: 352 TEGSEIHMGDLGGASSAKAEDGEIWTFSVRAFDSTLPERTINVNYEGFAEDVRVGDDLLV 411

Query: 304 DGGMVRFEVIEKIGPDVKCLCTDPGLLLPRANLTFWRDGHLVRERNAMLPTISSKDWLDI 363
           DGGMVRFEVIEKIGPDVKCLCTDPGLLLPRANLTFWRDGHLVRERNAMLPTISSKDWLDI
Sbjct: 412 DGGMVRFEVIEKIGPDVKCLCTDPGLLLPRANLTFWRDGHLVRERNAMLPTISSKDWLDI 471

Query: 364 DFGISEGVDFLAISFVKSAEVIKHLKSYIAARSRGSDISIIAKIESLDSLKNLEEIIVAS 423
           DFGISEGVDFLAISFVKSAEVIKHLKSYIAARSRGSDISIIAKIESLDSLKNLEEIIVAS
Sbjct: 472 DFGISEGVDFLAISFVKSAEVIKHLKSYIAARSRGSDISIIAKIESLDSLKNLEEIIVAS 531

Query: 424 DGAMVARGDLGAQIPLEQVPSVQQKVVQLCRRLNKPVIVASQLLESMIEYPTPTRAEVAD 483
           DGAMVARGDLGAQIPLEQVPSVQQKVVQLCRRLNKPVIVASQLLESMIEYPTPTRAEVAD
Sbjct: 532 DGAMVARGDLGAQIPLEQVPSVQQKVVQLCRRLNKPVIVASQLLESMIEYPTPTRAEVAD 591

Query: 484 VSEAVRQRADALMLSGESAMGQFPEKALTVLRSVSLRIEKWWRDEKRHEAMELPEVGSSY 543
           VSEAVRQRADALMLSGESAMGQFPEKALTVLRSVSLRIEKWWRDEKRHEAMELPEVGSSY
Sbjct: 592 VSEAVRQRADALMLSGESAMGQFPEKALTVLRSVSLRIEKWWRDEKRHEAMELPEVGSSY 651

Query: 544 SDSILEEICNSTAKMANNLEVDAIFVYTKTGHMASLLSRCRPDCPIFAFTSTTSVRRRLN 603
           SDSILEEICNSTAKMANNLEVDAIFVYTKTGHMASLLSRCRPDCPIFAFTSTTSVRRRLN
Sbjct: 652 SDSILEEICNSTAKMANNLEVDAIFVYTKTGHMASLLSRCRPDCPIFAFTSTTSVRRRLN 711

Query: 604 LQWGLIPFRLSFSDDMENNLNKTFMLLKARNLIKSGDLVLAVSDMLQSIQVMNVP 659
           LQWGLIPFRLSFSDDMENNLNKTFMLLKARNLIKSGDLVLAVSDMLQSIQVMNVP
Sbjct: 712 LQWGLIPFRLSFSDDMENNLNKTFMLLKARNLIKSGDLVLAVSDMLQSIQVMNVP 766

BLAST of Carg15878 vs. NCBI nr
Match: XP_023000477.1 (pyruvate kinase isozyme A, chloroplastic-like [Cucurbita maxima])

HSP 1 Score: 1146.0 bits (2963), Expect = 0.0e+00
Identity = 595/597 (99.66%), Postives = 596/597 (99.83%), Query Frame = 0

Query: 62  MAQSLQLFTSSTTRSPPISLPKHSLSKTPSSSTAAFRLPVSFNKFSIRASSSPDLNPLSS 121
           MAQSLQLFTSSTTRSP ISLPKHSLSKTPSSSTAAFRLPVSFNKFSIRASSSPDLNPLSS
Sbjct: 1   MAQSLQLFTSSTTRSPSISLPKHSLSKTPSSSTAAFRLPVSFNKFSIRASSSPDLNPLSS 60

Query: 122 ASTSQVLVSENGSSSGGGFVSTSATTREFVPAVSDGSSIDVDTVTEAELKENGFRSTRRT 181
           ASTSQVLVSENGSSSGGGFVSTSATTREFVPAVSDGSSIDVDTVTEAELKENGFRSTRRT
Sbjct: 61  ASTSQVLVSENGSSSGGGFVSTSATTREFVPAVSDGSSIDVDTVTEAELKENGFRSTRRT 120

Query: 182 KLVCTIGPVTCGFEQLEALAVGGMNVARINMCHGTREWHRMVIERVRRLNEEKGYAVAIM 241
           KLVCTIGPVTCGFEQLEALAVGGMNVARINMCHGTREWHRMVIERVRRLNEEKGYAVAIM
Sbjct: 121 KLVCTIGPVTCGFEQLEALAVGGMNVARINMCHGTREWHRMVIERVRRLNEEKGYAVAIM 180

Query: 242 MDTEGSEIHMGDLGGASSAKAEDGEIWTFSVRAFDSTLPERTINVNYEGFAEDVRVGDDL 301
           MDTEGSEIHMGDLGGASSAKAEDGEIWTFSVRAFDSTLPERTINVNYEGFAEDVRVGDDL
Sbjct: 181 MDTEGSEIHMGDLGGASSAKAEDGEIWTFSVRAFDSTLPERTINVNYEGFAEDVRVGDDL 240

Query: 302 LVDGGMVRFEVIEKIGPDVKCLCTDPGLLLPRANLTFWRDGHLVRERNAMLPTISSKDWL 361
           LVDGGMVRFEVIEKIGPDVKCLCTDPGLLLPRANLTFWRDGHLVRERNAMLPTISSKDWL
Sbjct: 241 LVDGGMVRFEVIEKIGPDVKCLCTDPGLLLPRANLTFWRDGHLVRERNAMLPTISSKDWL 300

Query: 362 DIDFGISEGVDFLAISFVKSAEVIKHLKSYIAARSRGSDISIIAKIESLDSLKNLEEIIV 421
           DIDFGI+EGVDFLAISFVKSAEVIKHLKSYIAARSRGSDISIIAKIESLDSLKNLEEIIV
Sbjct: 301 DIDFGIAEGVDFLAISFVKSAEVIKHLKSYIAARSRGSDISIIAKIESLDSLKNLEEIIV 360

Query: 422 ASDGAMVARGDLGAQIPLEQVPSVQQKVVQLCRRLNKPVIVASQLLESMIEYPTPTRAEV 481
           ASDGAMVARGDLGAQIPLEQVPSVQQKVVQLCRRLNKPVIVASQLLESMIEYPTPTRAEV
Sbjct: 361 ASDGAMVARGDLGAQIPLEQVPSVQQKVVQLCRRLNKPVIVASQLLESMIEYPTPTRAEV 420

Query: 482 ADVSEAVRQRADALMLSGESAMGQFPEKALTVLRSVSLRIEKWWRDEKRHEAMELPEVGS 541
           ADVSEAVRQRADALMLSGESAMGQFPEKALTVLRSVSLRIEKWWRDEKRHEAMELPEVGS
Sbjct: 421 ADVSEAVRQRADALMLSGESAMGQFPEKALTVLRSVSLRIEKWWRDEKRHEAMELPEVGS 480

Query: 542 SYSDSILEEICNSTAKMANNLEVDAIFVYTKTGHMASLLSRCRPDCPIFAFTSTTSVRRR 601
           SYSDSILEEICNSTAKMANNLEVDAIFVYTKTGHMASLLSRCRPDCPIFAFTSTTSVRRR
Sbjct: 481 SYSDSILEEICNSTAKMANNLEVDAIFVYTKTGHMASLLSRCRPDCPIFAFTSTTSVRRR 540

Query: 602 LNLQWGLIPFRLSFSDDMENNLNKTFMLLKARNLIKSGDLVLAVSDMLQSIQVMNVP 659
           LNLQWGLIPFRLSFSDDMENNLNKTFMLLKARNLIKSGDLVLAVSDMLQSIQVMNVP
Sbjct: 541 LNLQWGLIPFRLSFSDDMENNLNKTFMLLKARNLIKSGDLVLAVSDMLQSIQVMNVP 597

BLAST of Carg15878 vs. NCBI nr
Match: XP_023514423.1 (pyruvate kinase isozyme A, chloroplastic-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 1142.5 bits (2954), Expect = 0.0e+00
Identity = 592/597 (99.16%), Postives = 595/597 (99.66%), Query Frame = 0

Query: 62  MAQSLQLFTSSTTRSPPISLPKHSLSKTPSSSTAAFRLPVSFNKFSIRASSSPDLNPLSS 121
           MAQSLQLFTSSTTRSPPISLPKHSLSK PSSSTAAFRLPVSFNKFSIRA+S+PDLNPLSS
Sbjct: 1   MAQSLQLFTSSTTRSPPISLPKHSLSKPPSSSTAAFRLPVSFNKFSIRAASAPDLNPLSS 60

Query: 122 ASTSQVLVSENGSSSGGGFVSTSATTREFVPAVSDGSSIDVDTVTEAELKENGFRSTRRT 181
           ASTSQVLVSENGSSSGGGFVSTSA TREFVPAVSDGSSIDVDTVTEAELKENGFRSTRRT
Sbjct: 61  ASTSQVLVSENGSSSGGGFVSTSAATREFVPAVSDGSSIDVDTVTEAELKENGFRSTRRT 120

Query: 182 KLVCTIGPVTCGFEQLEALAVGGMNVARINMCHGTREWHRMVIERVRRLNEEKGYAVAIM 241
           KLVCTIGPVTCGFEQLEALAVGGMNVARINMCHGTREWHRMVIERVRRLNEEKGYAVAIM
Sbjct: 121 KLVCTIGPVTCGFEQLEALAVGGMNVARINMCHGTREWHRMVIERVRRLNEEKGYAVAIM 180

Query: 242 MDTEGSEIHMGDLGGASSAKAEDGEIWTFSVRAFDSTLPERTINVNYEGFAEDVRVGDDL 301
           MDTEGSEIHMGDLGGASSAKAEDGEIWTFSVRAFDSTLPERTINVNYEGFAEDVRVGDDL
Sbjct: 181 MDTEGSEIHMGDLGGASSAKAEDGEIWTFSVRAFDSTLPERTINVNYEGFAEDVRVGDDL 240

Query: 302 LVDGGMVRFEVIEKIGPDVKCLCTDPGLLLPRANLTFWRDGHLVRERNAMLPTISSKDWL 361
           LVDGGMVRFEVIEKIGPDVKCLCTDPGLLLPRANLTFWRDGHLVRERNAMLPTISSKDWL
Sbjct: 241 LVDGGMVRFEVIEKIGPDVKCLCTDPGLLLPRANLTFWRDGHLVRERNAMLPTISSKDWL 300

Query: 362 DIDFGISEGVDFLAISFVKSAEVIKHLKSYIAARSRGSDISIIAKIESLDSLKNLEEIIV 421
           DIDFGI+EGVDFLAISFVKSAEVIKHLKSYIAARSRGSDISIIAKIESLDSLKNLEEIIV
Sbjct: 301 DIDFGIAEGVDFLAISFVKSAEVIKHLKSYIAARSRGSDISIIAKIESLDSLKNLEEIIV 360

Query: 422 ASDGAMVARGDLGAQIPLEQVPSVQQKVVQLCRRLNKPVIVASQLLESMIEYPTPTRAEV 481
           ASDGAMVARGDLGAQIPLEQVPSVQQKVVQLCRRLNKPVIVASQLLESMIEYPTPTRAEV
Sbjct: 361 ASDGAMVARGDLGAQIPLEQVPSVQQKVVQLCRRLNKPVIVASQLLESMIEYPTPTRAEV 420

Query: 482 ADVSEAVRQRADALMLSGESAMGQFPEKALTVLRSVSLRIEKWWRDEKRHEAMELPEVGS 541
           ADVSEAVRQRADALMLSGESAMGQFPEKALTVLRSVSLRIEKWWRDEKRHEAMELPEVGS
Sbjct: 421 ADVSEAVRQRADALMLSGESAMGQFPEKALTVLRSVSLRIEKWWRDEKRHEAMELPEVGS 480

Query: 542 SYSDSILEEICNSTAKMANNLEVDAIFVYTKTGHMASLLSRCRPDCPIFAFTSTTSVRRR 601
           SYSDSILEEICNSTAKMANNLEVDAIFVYTKTGHMASLLSRCRPDCPIFAFTSTTSVRRR
Sbjct: 481 SYSDSILEEICNSTAKMANNLEVDAIFVYTKTGHMASLLSRCRPDCPIFAFTSTTSVRRR 540

Query: 602 LNLQWGLIPFRLSFSDDMENNLNKTFMLLKARNLIKSGDLVLAVSDMLQSIQVMNVP 659
           LNLQWGLIPFRLSFSDDMENNLNKTFMLLKARNLIKSGDLVLAVSDMLQSIQVMNVP
Sbjct: 541 LNLQWGLIPFRLSFSDDMENNLNKTFMLLKARNLIKSGDLVLAVSDMLQSIQVMNVP 597

BLAST of Carg15878 vs. NCBI nr
Match: XP_022930416.1 (pyruvate kinase isozyme A, chloroplastic-like [Cucurbita moschata])

HSP 1 Score: 1141.7 bits (2952), Expect = 0.0e+00
Identity = 593/597 (99.33%), Postives = 594/597 (99.50%), Query Frame = 0

Query: 62  MAQSLQLFTSSTTRSPPISLPKHSLSKTPSSSTAAFRLPVSFNKFSIRASSSPDLNPLSS 121
           MAQSLQLFTSSTTRSPPISL KHSLSK PSSSTA FRLPVSFN+FSIRASSSPDLNPLSS
Sbjct: 1   MAQSLQLFTSSTTRSPPISLSKHSLSKPPSSSTATFRLPVSFNQFSIRASSSPDLNPLSS 60

Query: 122 ASTSQVLVSENGSSSGGGFVSTSATTREFVPAVSDGSSIDVDTVTEAELKENGFRSTRRT 181
           ASTSQVLVSENGSSSGGGFVSTSATTREFVPAVSDGSSIDVDTVTEAELKENGFRSTRRT
Sbjct: 61  ASTSQVLVSENGSSSGGGFVSTSATTREFVPAVSDGSSIDVDTVTEAELKENGFRSTRRT 120

Query: 182 KLVCTIGPVTCGFEQLEALAVGGMNVARINMCHGTREWHRMVIERVRRLNEEKGYAVAIM 241
           KLVCTIGPVTCGFEQLEALAVGGMNVARINMCHGTREWHRMVIERVRRLNEEKGYAVAIM
Sbjct: 121 KLVCTIGPVTCGFEQLEALAVGGMNVARINMCHGTREWHRMVIERVRRLNEEKGYAVAIM 180

Query: 242 MDTEGSEIHMGDLGGASSAKAEDGEIWTFSVRAFDSTLPERTINVNYEGFAEDVRVGDDL 301
           MDTEGSEIHMGDLGGASSAKAEDGEIWTFSVRAFDSTLPERTINVNYEGFAEDVRVGDDL
Sbjct: 181 MDTEGSEIHMGDLGGASSAKAEDGEIWTFSVRAFDSTLPERTINVNYEGFAEDVRVGDDL 240

Query: 302 LVDGGMVRFEVIEKIGPDVKCLCTDPGLLLPRANLTFWRDGHLVRERNAMLPTISSKDWL 361
           LVDGGMVRFEVIEKIGPDVKCLCTDPGLLLPRANLTFWRDGHLVRERNAMLPTISSKDWL
Sbjct: 241 LVDGGMVRFEVIEKIGPDVKCLCTDPGLLLPRANLTFWRDGHLVRERNAMLPTISSKDWL 300

Query: 362 DIDFGISEGVDFLAISFVKSAEVIKHLKSYIAARSRGSDISIIAKIESLDSLKNLEEIIV 421
           DIDFGISEGVDFLAISFVKSAEVIKHLKSYIAARSRGSDISIIAKIESLDSLKNLEEIIV
Sbjct: 301 DIDFGISEGVDFLAISFVKSAEVIKHLKSYIAARSRGSDISIIAKIESLDSLKNLEEIIV 360

Query: 422 ASDGAMVARGDLGAQIPLEQVPSVQQKVVQLCRRLNKPVIVASQLLESMIEYPTPTRAEV 481
           ASDGAMVARGDLGAQIPLEQVPSVQQKVVQLCRRLNKPVIVASQLLESMIEYPTPTRAEV
Sbjct: 361 ASDGAMVARGDLGAQIPLEQVPSVQQKVVQLCRRLNKPVIVASQLLESMIEYPTPTRAEV 420

Query: 482 ADVSEAVRQRADALMLSGESAMGQFPEKALTVLRSVSLRIEKWWRDEKRHEAMELPEVGS 541
           ADVSEAVRQRADALMLSGESAMGQFPEKALTVLRSVSLRIEKWWRDEKRHEAMELPEVGS
Sbjct: 421 ADVSEAVRQRADALMLSGESAMGQFPEKALTVLRSVSLRIEKWWRDEKRHEAMELPEVGS 480

Query: 542 SYSDSILEEICNSTAKMANNLEVDAIFVYTKTGHMASLLSRCRPDCPIFAFTSTTSVRRR 601
           SYSDSILEEICNSTAKMANNLEVDAIFVYTKTGHMASLLSRCRPDCPIFAFTSTTSVRRR
Sbjct: 481 SYSDSILEEICNSTAKMANNLEVDAIFVYTKTGHMASLLSRCRPDCPIFAFTSTTSVRRR 540

Query: 602 LNLQWGLIPFRLSFSDDMENNLNKTFMLLKARNLIKSGDLVLAVSDMLQSIQVMNVP 659
           LNLQWGLIPFRLSFSDDMENNLNKTFMLLKARNLIKSGDLVLAVSDMLQSIQVMNVP
Sbjct: 541 LNLQWGLIPFRLSFSDDMENNLNKTFMLLKARNLIKSGDLVLAVSDMLQSIQVMNVP 597

BLAST of Carg15878 vs. ExPASy Swiss-Prot
Match: Q43117 (Pyruvate kinase isozyme A, chloroplastic OS=Ricinus communis OX=3988 PE=1 SV=1)

HSP 1 Score: 922.2 bits (2382), Expect = 3.4e-267
Identity = 484/599 (80.80%), Postives = 527/599 (87.98%), Query Frame = 0

Query: 62  MAQSLQLFTSSTTRSPPISLPKHSLSKTP-SSSTAAFRLPV-SFNKFSIRASSSPDLNPL 121
           M+QSL         SP ++  K    K P    T+  R PV ++   SI+AS+SP     
Sbjct: 1   MSQSLHF-------SPNLTFAKQPFPKLPLPFPTSNSRYPVNNYKSLSIKASTSP----- 60

Query: 122 SSASTSQVLVSENGSSSGGGFVSTSATTREFVPAVSDGSSIDVDTVTEAELKENGFRSTR 181
           SS+S  QVLV++NG+ + G   + +  +      VSD SSI+VD VTE ELKENGFRSTR
Sbjct: 61  SSSSDPQVLVADNGTGNSGVLYNNNNKS----VTVSDPSSIEVDAVTETELKENGFRSTR 120

Query: 182 RTKLVCTIGPVTCGFEQLEALAVGGMNVARINMCHGTREWHRMVIERVRRLNEEKGYAVA 241
           RTKLVCTIGP TCGFE+LEALAVGGMNVARINMCHGTREWH+ VIERVRRLNEEKG+AVA
Sbjct: 121 RTKLVCTIGPATCGFEELEALAVGGMNVARINMCHGTREWHKSVIERVRRLNEEKGFAVA 180

Query: 242 IMMDTEGSEIHMGDLGGASSAKAEDGEIWTFSVRAFDSTLPERTINVNYEGFAEDVRVGD 301
           IMMDTEGSEIHMGDLGGASSAKAEDGEIWTFSVRA+DS  PERTINVNY+GFAEDV+VGD
Sbjct: 181 IMMDTEGSEIHMGDLGGASSAKAEDGEIWTFSVRAYDSPRPERTINVNYDGFAEDVKVGD 240

Query: 302 DLLVDGGMVRFEVIEKIGPDVKCLCTDPGLLLPRANLTFWRDGHLVRERNAMLPTISSKD 361
           +LLVDGGMVRFEVIEKIGPDVKC CTDPGLLLPRANLTFWRDG LVRERNAMLPTISSKD
Sbjct: 241 ELLVDGGMVRFEVIEKIGPDVKCRCTDPGLLLPRANLTFWRDGSLVRERNAMLPTISSKD 300

Query: 362 WLDIDFGISEGVDFLAISFVKSAEVIKHLKSYIAARSRGSDISIIAKIESLDSLKNLEEI 421
           WLDIDFGI+EGVDF+AISFVKSAEVI HLKSYIAARSR SDI++IAKIES+DSLKNLEEI
Sbjct: 301 WLDIDFGIAEGVDFIAISFVKSAEVINHLKSYIAARSRDSDIAVIAKIESIDSLKNLEEI 360

Query: 422 IVASDGAMVARGDLGAQIPLEQVPSVQQKVVQLCRRLNKPVIVASQLLESMIEYPTPTRA 481
           I ASDGAMVARGDLGAQIPLEQVPS QQ +VQ+CR+LNKPVIVASQLLESMIEYPTPTRA
Sbjct: 361 IRASDGAMVARGDLGAQIPLEQVPSAQQNIVQVCRQLNKPVIVASQLLESMIEYPTPTRA 420

Query: 482 EVADVSEAVRQRADALMLSGESAMGQFPEKALTVLRSVSLRIEKWWRDEKRHEAMELPEV 541
           EVADVSEAVRQRADALMLSGESAMGQ+PEKAL VLRSVS+RIEKWWR+EK HEAMELP +
Sbjct: 421 EVADVSEAVRQRADALMLSGESAMGQYPEKALAVLRSVSVRIEKWWREEKHHEAMELPAI 480

Query: 542 GSSYSDSILEEICNSTAKMANNLEVDAIFVYTKTGHMASLLSRCRPDCPIFAFTSTTSVR 601
           GS+YSDSI EEICNS AKMANNL VDA+FVYTK GHMASLLSRCRPDCPIFAFT+TTSVR
Sbjct: 481 GSTYSDSISEEICNSAAKMANNLGVDALFVYTKDGHMASLLSRCRPDCPIFAFTTTTSVR 540

Query: 602 RRLNLQWGLIPFRLSFSDDMENNLNKTFMLLKARNLIKSGDLVLAVSDMLQSIQVMNVP 659
           RRLNLQWGLIPFRLSF+DDME+NLNKTF LLKAR +IKSGDLV+AVSDMLQSIQVMNVP
Sbjct: 541 RRLNLQWGLIPFRLSFADDMESNLNKTFSLLKARGMIKSGDLVIAVSDMLQSIQVMNVP 583

BLAST of Carg15878 vs. ExPASy Swiss-Prot
Match: Q40545 (Pyruvate kinase isozyme A, chloroplastic OS=Nicotiana tabacum OX=4097 PE=2 SV=1)

HSP 1 Score: 916.4 bits (2367), Expect = 1.9e-265
Identity = 481/602 (79.90%), Postives = 527/602 (87.54%), Query Frame = 0

Query: 62  MAQSLQLFTSSTTRSP---PISLPKHSLSKTPSSSTAAFRLPVSFNKFSIRASSSPDLNP 121
           M+Q+L  F SS++RSP    IS P    S  PS+ +    +  S     + ASS+   + 
Sbjct: 1   MSQALNFFVSSSSRSPATFTISRP----SVFPSTGSLRLLVKKSLRTLVVEASSAA-ASD 60

Query: 122 LSSASTSQVLVSENGSSSGGGFVS--TSATTREFVPAVSDGSSIDVDTVTEAELKENGFR 181
           L    +S VLVSENGS   GG +S  T    R   P  +D SSI+VDTVTEAELKENGFR
Sbjct: 61  LDEPQSSPVLVSENGS---GGVLSSATQEYGRNAAPG-TDSSSIEVDTVTEAELKENGFR 120

Query: 182 STRRTKLVCTIGPVTCGFEQLEALAVGGMNVARINMCHGTREWHRMVIERVRRLNEEKGY 241
           STRRTKL+CTIGP TCGFEQLE LA GGMNVARINMCHGTREWHRMVIER+RRLNEEKG+
Sbjct: 121 STRRTKLICTIGPATCGFEQLERLAEGGMNVARINMCHGTREWHRMVIERLRRLNEEKGF 180

Query: 242 AVAIMMDTEGSEIHMGDLGGASSAKAEDGEIWTFSVRAFDSTLPERTINVNYEGFAEDVR 301
           AVAIMMDTEGSEIHMGDLGGASSAKAEDGEIW F+VR+FD  LPERT+ VNY+GFAEDV+
Sbjct: 181 AVAIMMDTEGSEIHMGDLGGASSAKAEDGEIWNFTVRSFDPPLPERTVTVNYDGFAEDVK 240

Query: 302 VGDDLLVDGGMVRFEVIEKIGPDVKCLCTDPGLLLPRANLTFWRDGHLVRERNAMLPTIS 361
           VGD+LLVDGGMVRFEVIEKIGPDVKCLCTDPGLLLPRANLTFWRDG LVRERNAMLPTIS
Sbjct: 241 VGDELLVDGGMVRFEVIEKIGPDVKCLCTDPGLLLPRANLTFWRDGKLVRERNAMLPTIS 300

Query: 362 SKDWLDIDFGISEGVDFLAISFVKSAEVIKHLKSYIAARSRGSDISIIAKIESLDSLKNL 421
           SKDWLDIDFGI+EGVDF+A+SFVKSAEVIKHLKSYI AR+R SDIS+IAKIES+DSLKNL
Sbjct: 301 SKDWLDIDFGIAEGVDFIAVSFVKSAEVIKHLKSYIQARARDSDISVIAKIESIDSLKNL 360

Query: 422 EEIIVASDGAMVARGDLGAQIPLEQVPSVQQKVVQLCRRLNKPVIVASQLLESMIEYPTP 481
           EEII ASDGAMVARGDLGAQIPLEQVPS QQK+VQ+CR+LN+PVIVASQLLESMIEYP P
Sbjct: 361 EEIIQASDGAMVARGDLGAQIPLEQVPSEQQKIVQICRQLNRPVIVASQLLESMIEYPIP 420

Query: 482 TRAEVADVSEAVRQRADALMLSGESAMGQFPEKALTVLRSVSLRIEKWWRDEKRHEAMEL 541
           TRAEVADVSEAVRQR DALMLSGESAMGQFPEKALTVLRSVSLRIE+ WR++KRHE +EL
Sbjct: 421 TRAEVADVSEAVRQRGDALMLSGESAMGQFPEKALTVLRSVSLRIERMWREQKRHEVIEL 480

Query: 542 PEVGSSYSDSILEEICNSTAKMANNLEVDAIFVYTKTGHMASLLSRCRPDCPIFAFTSTT 601
           P + SS+SDSI EEICNS AKMANNLEVDA+FVYTK GHMASLLSRCRPDCPIFAFT+TT
Sbjct: 481 PSIASSFSDSISEEICNSAAKMANNLEVDALFVYTKNGHMASLLSRCRPDCPIFAFTTTT 540

Query: 602 SVRRRLNLQWGLIPFRLSFSDDMENNLNKTFMLLKARNLIKSGDLVLAVSDMLQSIQVMN 659
           SVRRRLNLQWGL+PFRLSFSDDME+NLNKTF LLKAR +IKSGDL++AVSDMLQSIQVMN
Sbjct: 541 SVRRRLNLQWGLMPFRLSFSDDMESNLNKTFSLLKARGMIKSGDLIIAVSDMLQSIQVMN 593

BLAST of Carg15878 vs. ExPASy Swiss-Prot
Match: Q9LIK0 (Plastidial pyruvate kinase 1, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=PKP1 PE=1 SV=1)

HSP 1 Score: 912.9 bits (2358), Expect = 2.1e-264
Identity = 474/602 (78.74%), Postives = 531/602 (88.21%), Query Frame = 0

Query: 62  MAQSLQLFTSSTTRSPPISLPKHSLSKTPSSSTAAFRLPVSFNKF-SIRASS----SPDL 121
           M+QS+Q  T S T  P +    HS    P SS +  R P++  K+ SIRASS    SPDL
Sbjct: 1   MSQSIQFSTPSHT--PHLLHLPHSQFNRPLSSISFRRFPLTTIKYTSIRASSSSSPSPDL 60

Query: 122 NPLSSASTSQVLVSENGSSSGGGFVSTSATTREFVPAVSDGSSIDVDTVTEAELKENGFR 181
           +  SS+S+SQVL+S NG+    G V +   +       +D S I+VDTVTEAELKENGFR
Sbjct: 61  DSSSSSSSSQVLLSPNGT----GAVKSDERSVVATAVTTDTSGIEVDTVTEAELKENGFR 120

Query: 182 STRRTKLVCTIGPVTCGFEQLEALAVGGMNVARINMCHGTREWHRMVIERVRRLNEEKGY 241
           STRRTKL+CTIGP TCGFEQLEALAVGGMNVAR+NMCHGTR+WHR VI  VRRLNEEKG+
Sbjct: 121 STRRTKLICTIGPATCGFEQLEALAVGGMNVARLNMCHGTRDWHRGVIRSVRRLNEEKGF 180

Query: 242 AVAIMMDTEGSEIHMGDLGGASSAKAEDGEIWTFSVRAFDSTLPERTINVNYEGFAEDVR 301
           AVAIMMDTEGSEIHMGDLGG +SAKAEDGE+WTF+VRAFDS+ PERTI+V+Y+GFAEDVR
Sbjct: 181 AVAIMMDTEGSEIHMGDLGGEASAKAEDGEVWTFTVRAFDSSRPERTISVSYDGFAEDVR 240

Query: 302 VGDDLLVDGGMVRFEVIEKIGPDVKCLCTDPGLLLPRANLTFWRDGHLVRERNAMLPTIS 361
           VGD+LLVDGGMVRFEVIEKIGPDVKCLCTDPGLLLPRANLTFWRDG LVRERNAMLPTIS
Sbjct: 241 VGDELLVDGGMVRFEVIEKIGPDVKCLCTDPGLLLPRANLTFWRDGSLVRERNAMLPTIS 300

Query: 362 SKDWLDIDFGISEGVDFLAISFVKSAEVIKHLKSYIAARSRGSDISIIAKIESLDSLKNL 421
           SKDWLDIDFGI+EGVDF+A+SFVKSAEVI HLKSY+AARSRG +I +IAKIES+DSL NL
Sbjct: 301 SKDWLDIDFGIAEGVDFIAVSFVKSAEVINHLKSYLAARSRGGEIGVIAKIESIDSLTNL 360

Query: 422 EEIIVASDGAMVARGDLGAQIPLEQVPSVQQKVVQLCRRLNKPVIVASQLLESMIEYPTP 481
           EEII+ASDGAMVARGDLGAQIPLEQVP+ QQ++VQ+CR LNKPVIVASQLLESMIEYPTP
Sbjct: 361 EEIILASDGAMVARGDLGAQIPLEQVPAAQQRIVQVCRALNKPVIVASQLLESMIEYPTP 420

Query: 482 TRAEVADVSEAVRQRADALMLSGESAMGQFPEKALTVLRSVSLRIEKWWRDEKRHEAMEL 541
           TRAEVADVSEAVRQR+DALMLSGESAMGQFP+KALTVLR+VSLRIE+WWR+EKRHE++ L
Sbjct: 421 TRAEVADVSEAVRQRSDALMLSGESAMGQFPDKALTVLRTVSLRIERWWREEKRHESVPL 480

Query: 542 PEVGSSYSDSILEEICNSTAKMANNLEVDAIFVYTKTGHMASLLSRCRPDCPIFAFTSTT 601
             +GSS+SD I EEICNS AKMANNL VDA+FVYT +GHMASL+SRCRPDCPIFAFT+TT
Sbjct: 481 QAIGSSFSDKISEEICNSAAKMANNLGVDAVFVYTTSGHMASLVSRCRPDCPIFAFTTTT 540

Query: 602 SVRRRLNLQWGLIPFRLSFSDDMENNLNKTFMLLKARNLIKSGDLVLAVSDMLQSIQVMN 659
           SVRRRLNLQWGLIPFRLSFSDDME+NLNKTF LLK+R +IKSGDLV+AVSDMLQSIQVMN
Sbjct: 541 SVRRRLNLQWGLIPFRLSFSDDMESNLNKTFSLLKSRGMIKSGDLVIAVSDMLQSIQVMN 596

BLAST of Carg15878 vs. ExPASy Swiss-Prot
Match: Q40546 (Pyruvate kinase isozyme G, chloroplastic OS=Nicotiana tabacum OX=4097 PE=2 SV=1)

HSP 1 Score: 373.2 bits (957), Expect = 5.9e-102
Identity = 212/477 (44.44%), Postives = 299/477 (62.68%), Query Frame = 0

Query: 177 STRRTKLVCTIGPVTCGFEQLEALAVGGMNVARINMCHGTREWHRMVIERVRRLNEE-KG 236
           S R+TK+VCTIGP T   E +  LA  GMNVAR+NM HG    H+  I+ V+  N + + 
Sbjct: 89  SPRKTKIVCTIGPSTSSREMIWKLAEAGMNVARLNMSHGDHASHQRTIDLVKEYNAQFED 148

Query: 237 YAVAIMMDTEGSEIHMGDLGGASSAKAEDGEIWTFSVRAFDSTLPERTINVNYEGFAEDV 296
             +AIM+DT+G E+  GD+        ++G+ + FS++   ST  E T++VNY+ F  DV
Sbjct: 149 KVIAIMLDTKGPEVISGDV--PKPILLKEGQEFNFSIKRGVST--EDTVSVNYDDFINDV 208

Query: 297 RVGDDLLVDGGMVRFEVIEKIGPDVKCLCTDPGLLLPRANLTFWRDGHLVRERNAMLPTI 356
             GD LLVDGGM+   V  K    VKC   D G L  R +L        VR ++A LP+I
Sbjct: 209 EAGDILLVDGGMMSLAVKSKTSDIVKCEVIDGGELKSRRHLN-------VRGKSATLPSI 268

Query: 357 SSKDWLDIDFGISEGVDFLAISFVKSAEVIKHLKSYIAARSRGSDISIIAKIESLDSLKN 416
           + KDW DI FG++  VDF A+SFVK A+V+  LK Y+  +S  +DI +I KIES DS+ N
Sbjct: 269 TEKDWDDIKFGVNNQVDFYAVSFVKDAKVVHELKDYL--KSCNADIHVIVKIESADSIPN 328

Query: 417 LEEIIVASDGAMVARGDLGAQIPLEQVPSVQQKVVQLCRRLNKPVIVASQLLESMIEYPT 476
           L  II ASDGAMVARGDLGA++P+E+VP +Q+ +++ C+ + KPVIVA+ +LESMI++PT
Sbjct: 329 LHSIISASDGAMVARGDLGAELPIEEVPLLQEDIIRRCQSMQKPVIVATNMLESMIDHPT 388

Query: 477 PTRAEVADVSEAVRQRADALMLSGESAMGQFPEKALTVLRSVSLRIEKWWRDEKRHEAME 536
           PTRAEV+D+S AVR+ ADA+MLSGE+A G++P KA+ V+  V+LR E         ++  
Sbjct: 389 PTRAEVSDISIAVREGADAVMLSGETAHGKYPLKAVKVMHIVALRTE-----SSLQKSTS 448

Query: 537 LPEVGSSYSDSILEEICNSTAKMANNLEVDAIFVYTKTGHMASLLSRCRPDCPIFAFTST 596
            P   ++Y   + E     ++ MAN L    I V+T+TG MA +LS  RP   +FAFT+ 
Sbjct: 449 SPSQSAAYKSHMGEMFAFHSSSMANTLST-PIIVFTRTGSMAIILSHNRPSSTVFAFTNN 508

Query: 597 TSVRRRLNLQWGLIPFRLSFSDDMENNLNKTFMLLKARNLIKSGDLVLAVSDMLQSI 653
             V++RL L  G++P  + FS D E   ++   LL +++L+K G  V  V    Q I
Sbjct: 509 ERVKQRLALYHGVVPIYMEFSSDAEETFSRAIKLLLSKSLVKDGQYVTLVQSGAQPI 546

BLAST of Carg15878 vs. ExPASy Swiss-Prot
Match: Q9FLW9 (Plastidial pyruvate kinase 2 OS=Arabidopsis thaliana OX=3702 GN=PKP2 PE=1 SV=1)

HSP 1 Score: 364.8 bits (935), Expect = 2.1e-99
Identity = 208/475 (43.79%), Postives = 291/475 (61.26%), Query Frame = 0

Query: 179 RRTKLVCTIGPVTCGFEQLEALAVGGMNVARINMCHGTREWHRMVIERVRRLN-EEKGYA 238
           R+TK+VCT+GP T   E +  LA  GMNVAR+NM HG    H+ VI+ V+  N + K   
Sbjct: 110 RKTKIVCTVGPSTNTREMIWKLAEAGMNVARMNMSHGDHASHKKVIDLVKEYNAQTKDNT 169

Query: 239 VAIMMDTEGSEIHMGDLGGASSAKAEDGEIWTFSVRAFDSTLPERTINVNYEGFAEDVRV 298
           +AIM+DT+G E+  GDL        + G+ +TF++    ST     ++VNY+ F  DV  
Sbjct: 170 IAIMLDTKGPEVRSGDL--PQPIMLDPGQEFTFTIERGVST--PSCVSVNYDDFVNDVEA 229

Query: 299 GDDLLVDGGMVRFEVIEKIGPDVKCLCTDPGLLLPRANLTFWRDGHLVRERNAMLPTISS 358
           GD LLVDGGM+ F V  K    VKC   D G L  R +L        VR ++A LP+I+ 
Sbjct: 230 GDMLLVDGGMMSFMVKSKTKDSVKCEVVDGGELKSRRHLN-------VRGKSATLPSITE 289

Query: 359 KDWLDIDFGISEGVDFLAISFVKSAEVIKHLKSYIAARSRGSDISIIAKIESLDSLKNLE 418
           KDW DI FG+   VDF A+SFVK A+V+  LK Y+  ++ G+DI +I KIES DS+ NL 
Sbjct: 290 KDWEDIKFGVENKVDFYAVSFVKDAQVVHELKKYL--QNSGADIHVIVKIESADSIPNLH 349

Query: 419 EIIVASDGAMVARGDLGAQIPLEQVPSVQQKVVQLCRRLNKPVIVASQLLESMIEYPTPT 478
            II ASDGAMVARGDLGA++P+E+VP +Q++++ LCR + K VIVA+ +LESMI +PTPT
Sbjct: 350 SIITASDGAMVARGDLGAELPIEEVPILQEEIINLCRSMGKAVIVATNMLESMIVHPTPT 409

Query: 479 RAEVADVSEAVRQRADALMLSGESAMGQFPEKALTVLRSVSLRIEKWWRDEKRHEAMELP 538
           RAEV+D++ AVR+ ADA+MLSGE+A G+FP KA  V+ +V+LR E      +       P
Sbjct: 410 RAEVSDIAIAVREGADAVMLSGETAHGKFPLKAAGVMHTVALRTEATITSGEMP-----P 469

Query: 539 EVGSSYSDSILEEICNSTAKMANNLEVDAIFVYTKTGHMASLLSRCRPDCPIFAFTSTTS 598
            +G ++ + + E        M+N L    + V+T+TG MA LLS  RP   I+AFT+   
Sbjct: 470 NLGQAFKNHMSEMFAYHATMMSNTLGTSTV-VFTRTGFMAILLSHYRPSGTIYAFTNEKK 529

Query: 599 VRRRLNLQWGLIPFRLSFSDDMENNLNKTFMLLKARNLIKSGDLVLAVSDMLQSI 653
           +++RL L  G+ P  + F+DD E         L  + ++K G+ +  V    Q I
Sbjct: 530 IQQRLALYQGVCPIYMEFTDDAEETFANALATLLKQGMVKKGEEIAIVQSGTQPI 565

BLAST of Carg15878 vs. ExPASy TrEMBL
Match: A0A6J1KDQ9 (Pyruvate kinase OS=Cucurbita maxima OX=3661 GN=LOC111494735 PE=3 SV=1)

HSP 1 Score: 1146.0 bits (2963), Expect = 0.0e+00
Identity = 595/597 (99.66%), Postives = 596/597 (99.83%), Query Frame = 0

Query: 62  MAQSLQLFTSSTTRSPPISLPKHSLSKTPSSSTAAFRLPVSFNKFSIRASSSPDLNPLSS 121
           MAQSLQLFTSSTTRSP ISLPKHSLSKTPSSSTAAFRLPVSFNKFSIRASSSPDLNPLSS
Sbjct: 1   MAQSLQLFTSSTTRSPSISLPKHSLSKTPSSSTAAFRLPVSFNKFSIRASSSPDLNPLSS 60

Query: 122 ASTSQVLVSENGSSSGGGFVSTSATTREFVPAVSDGSSIDVDTVTEAELKENGFRSTRRT 181
           ASTSQVLVSENGSSSGGGFVSTSATTREFVPAVSDGSSIDVDTVTEAELKENGFRSTRRT
Sbjct: 61  ASTSQVLVSENGSSSGGGFVSTSATTREFVPAVSDGSSIDVDTVTEAELKENGFRSTRRT 120

Query: 182 KLVCTIGPVTCGFEQLEALAVGGMNVARINMCHGTREWHRMVIERVRRLNEEKGYAVAIM 241
           KLVCTIGPVTCGFEQLEALAVGGMNVARINMCHGTREWHRMVIERVRRLNEEKGYAVAIM
Sbjct: 121 KLVCTIGPVTCGFEQLEALAVGGMNVARINMCHGTREWHRMVIERVRRLNEEKGYAVAIM 180

Query: 242 MDTEGSEIHMGDLGGASSAKAEDGEIWTFSVRAFDSTLPERTINVNYEGFAEDVRVGDDL 301
           MDTEGSEIHMGDLGGASSAKAEDGEIWTFSVRAFDSTLPERTINVNYEGFAEDVRVGDDL
Sbjct: 181 MDTEGSEIHMGDLGGASSAKAEDGEIWTFSVRAFDSTLPERTINVNYEGFAEDVRVGDDL 240

Query: 302 LVDGGMVRFEVIEKIGPDVKCLCTDPGLLLPRANLTFWRDGHLVRERNAMLPTISSKDWL 361
           LVDGGMVRFEVIEKIGPDVKCLCTDPGLLLPRANLTFWRDGHLVRERNAMLPTISSKDWL
Sbjct: 241 LVDGGMVRFEVIEKIGPDVKCLCTDPGLLLPRANLTFWRDGHLVRERNAMLPTISSKDWL 300

Query: 362 DIDFGISEGVDFLAISFVKSAEVIKHLKSYIAARSRGSDISIIAKIESLDSLKNLEEIIV 421
           DIDFGI+EGVDFLAISFVKSAEVIKHLKSYIAARSRGSDISIIAKIESLDSLKNLEEIIV
Sbjct: 301 DIDFGIAEGVDFLAISFVKSAEVIKHLKSYIAARSRGSDISIIAKIESLDSLKNLEEIIV 360

Query: 422 ASDGAMVARGDLGAQIPLEQVPSVQQKVVQLCRRLNKPVIVASQLLESMIEYPTPTRAEV 481
           ASDGAMVARGDLGAQIPLEQVPSVQQKVVQLCRRLNKPVIVASQLLESMIEYPTPTRAEV
Sbjct: 361 ASDGAMVARGDLGAQIPLEQVPSVQQKVVQLCRRLNKPVIVASQLLESMIEYPTPTRAEV 420

Query: 482 ADVSEAVRQRADALMLSGESAMGQFPEKALTVLRSVSLRIEKWWRDEKRHEAMELPEVGS 541
           ADVSEAVRQRADALMLSGESAMGQFPEKALTVLRSVSLRIEKWWRDEKRHEAMELPEVGS
Sbjct: 421 ADVSEAVRQRADALMLSGESAMGQFPEKALTVLRSVSLRIEKWWRDEKRHEAMELPEVGS 480

Query: 542 SYSDSILEEICNSTAKMANNLEVDAIFVYTKTGHMASLLSRCRPDCPIFAFTSTTSVRRR 601
           SYSDSILEEICNSTAKMANNLEVDAIFVYTKTGHMASLLSRCRPDCPIFAFTSTTSVRRR
Sbjct: 481 SYSDSILEEICNSTAKMANNLEVDAIFVYTKTGHMASLLSRCRPDCPIFAFTSTTSVRRR 540

Query: 602 LNLQWGLIPFRLSFSDDMENNLNKTFMLLKARNLIKSGDLVLAVSDMLQSIQVMNVP 659
           LNLQWGLIPFRLSFSDDMENNLNKTFMLLKARNLIKSGDLVLAVSDMLQSIQVMNVP
Sbjct: 541 LNLQWGLIPFRLSFSDDMENNLNKTFMLLKARNLIKSGDLVLAVSDMLQSIQVMNVP 597

BLAST of Carg15878 vs. ExPASy TrEMBL
Match: A0A6J1EQW0 (Pyruvate kinase OS=Cucurbita moschata OX=3662 GN=LOC111436871 PE=3 SV=1)

HSP 1 Score: 1141.7 bits (2952), Expect = 0.0e+00
Identity = 593/597 (99.33%), Postives = 594/597 (99.50%), Query Frame = 0

Query: 62  MAQSLQLFTSSTTRSPPISLPKHSLSKTPSSSTAAFRLPVSFNKFSIRASSSPDLNPLSS 121
           MAQSLQLFTSSTTRSPPISL KHSLSK PSSSTA FRLPVSFN+FSIRASSSPDLNPLSS
Sbjct: 1   MAQSLQLFTSSTTRSPPISLSKHSLSKPPSSSTATFRLPVSFNQFSIRASSSPDLNPLSS 60

Query: 122 ASTSQVLVSENGSSSGGGFVSTSATTREFVPAVSDGSSIDVDTVTEAELKENGFRSTRRT 181
           ASTSQVLVSENGSSSGGGFVSTSATTREFVPAVSDGSSIDVDTVTEAELKENGFRSTRRT
Sbjct: 61  ASTSQVLVSENGSSSGGGFVSTSATTREFVPAVSDGSSIDVDTVTEAELKENGFRSTRRT 120

Query: 182 KLVCTIGPVTCGFEQLEALAVGGMNVARINMCHGTREWHRMVIERVRRLNEEKGYAVAIM 241
           KLVCTIGPVTCGFEQLEALAVGGMNVARINMCHGTREWHRMVIERVRRLNEEKGYAVAIM
Sbjct: 121 KLVCTIGPVTCGFEQLEALAVGGMNVARINMCHGTREWHRMVIERVRRLNEEKGYAVAIM 180

Query: 242 MDTEGSEIHMGDLGGASSAKAEDGEIWTFSVRAFDSTLPERTINVNYEGFAEDVRVGDDL 301
           MDTEGSEIHMGDLGGASSAKAEDGEIWTFSVRAFDSTLPERTINVNYEGFAEDVRVGDDL
Sbjct: 181 MDTEGSEIHMGDLGGASSAKAEDGEIWTFSVRAFDSTLPERTINVNYEGFAEDVRVGDDL 240

Query: 302 LVDGGMVRFEVIEKIGPDVKCLCTDPGLLLPRANLTFWRDGHLVRERNAMLPTISSKDWL 361
           LVDGGMVRFEVIEKIGPDVKCLCTDPGLLLPRANLTFWRDGHLVRERNAMLPTISSKDWL
Sbjct: 241 LVDGGMVRFEVIEKIGPDVKCLCTDPGLLLPRANLTFWRDGHLVRERNAMLPTISSKDWL 300

Query: 362 DIDFGISEGVDFLAISFVKSAEVIKHLKSYIAARSRGSDISIIAKIESLDSLKNLEEIIV 421
           DIDFGISEGVDFLAISFVKSAEVIKHLKSYIAARSRGSDISIIAKIESLDSLKNLEEIIV
Sbjct: 301 DIDFGISEGVDFLAISFVKSAEVIKHLKSYIAARSRGSDISIIAKIESLDSLKNLEEIIV 360

Query: 422 ASDGAMVARGDLGAQIPLEQVPSVQQKVVQLCRRLNKPVIVASQLLESMIEYPTPTRAEV 481
           ASDGAMVARGDLGAQIPLEQVPSVQQKVVQLCRRLNKPVIVASQLLESMIEYPTPTRAEV
Sbjct: 361 ASDGAMVARGDLGAQIPLEQVPSVQQKVVQLCRRLNKPVIVASQLLESMIEYPTPTRAEV 420

Query: 482 ADVSEAVRQRADALMLSGESAMGQFPEKALTVLRSVSLRIEKWWRDEKRHEAMELPEVGS 541
           ADVSEAVRQRADALMLSGESAMGQFPEKALTVLRSVSLRIEKWWRDEKRHEAMELPEVGS
Sbjct: 421 ADVSEAVRQRADALMLSGESAMGQFPEKALTVLRSVSLRIEKWWRDEKRHEAMELPEVGS 480

Query: 542 SYSDSILEEICNSTAKMANNLEVDAIFVYTKTGHMASLLSRCRPDCPIFAFTSTTSVRRR 601
           SYSDSILEEICNSTAKMANNLEVDAIFVYTKTGHMASLLSRCRPDCPIFAFTSTTSVRRR
Sbjct: 481 SYSDSILEEICNSTAKMANNLEVDAIFVYTKTGHMASLLSRCRPDCPIFAFTSTTSVRRR 540

Query: 602 LNLQWGLIPFRLSFSDDMENNLNKTFMLLKARNLIKSGDLVLAVSDMLQSIQVMNVP 659
           LNLQWGLIPFRLSFSDDMENNLNKTFMLLKARNLIKSGDLVLAVSDMLQSIQVMNVP
Sbjct: 541 LNLQWGLIPFRLSFSDDMENNLNKTFMLLKARNLIKSGDLVLAVSDMLQSIQVMNVP 597

BLAST of Carg15878 vs. ExPASy TrEMBL
Match: A0A0A0LKS3 (Pyruvate kinase OS=Cucumis sativus OX=3659 GN=Csa_2G215480 PE=3 SV=1)

HSP 1 Score: 1058.5 bits (2736), Expect = 1.1e-305
Identity = 551/597 (92.29%), Postives = 570/597 (95.48%), Query Frame = 0

Query: 62  MAQSLQLFTSSTTRSPPISLPKHSLSKTPSSSTAAFRLPVSFNKFSIRASSSPDLNPLSS 121
           M QSLQLFTSS      ISLPKHS+SK PSSS+AAFR P SF+K SIRASSS DLNPLSS
Sbjct: 1   MTQSLQLFTSSA-----ISLPKHSISK-PSSSSAAFRFPFSFSKSSIRASSSSDLNPLSS 60

Query: 122 ASTSQVLVSENGSSSGGGFVSTSATTREFVPAVSDGSSIDVDTVTEAELKENGFRSTRRT 181
           +STSQVLVSENGSSSGGG V +SA TREFV   SD +SI+VD VTEAELKENGFRSTRRT
Sbjct: 61  SSTSQVLVSENGSSSGGGVVFSSAATREFVSVASDSTSIEVDAVTEAELKENGFRSTRRT 120

Query: 182 KLVCTIGPVTCGFEQLEALAVGGMNVARINMCHGTREWHRMVIERVRRLNEEKGYAVAIM 241
           KLVCTIGP TCGFEQLEALAVGGMNVARINMCHGTR+WHR VIERVRRLN+ KGYAVAIM
Sbjct: 121 KLVCTIGPATCGFEQLEALAVGGMNVARINMCHGTRDWHRTVIERVRRLNDGKGYAVAIM 180

Query: 242 MDTEGSEIHMGDLGGASSAKAEDGEIWTFSVRAFDSTLPERTINVNYEGFAEDVRVGDDL 301
           MDTEGSEIHMGDLGGASSAKAEDGEIWTFSVRAFDSTLPERTINVNYEGFAEDVRVGDDL
Sbjct: 181 MDTEGSEIHMGDLGGASSAKAEDGEIWTFSVRAFDSTLPERTINVNYEGFAEDVRVGDDL 240

Query: 302 LVDGGMVRFEVIEKIGPDVKCLCTDPGLLLPRANLTFWRDGHLVRERNAMLPTISSKDWL 361
           LVDGGMVRFEVIEKIGPDVKCLCTDPGLLLPRANLTFWRDGHLVRERNAMLPTISSKDWL
Sbjct: 241 LVDGGMVRFEVIEKIGPDVKCLCTDPGLLLPRANLTFWRDGHLVRERNAMLPTISSKDWL 300

Query: 362 DIDFGISEGVDFLAISFVKSAEVIKHLKSYIAARSRGSDISIIAKIESLDSLKNLEEIIV 421
           DIDFGI+EGVDFLAISFVKSAEVIKHLKSYIAARSRGSDISIIAKIESLDSLKNLEEII+
Sbjct: 301 DIDFGIAEGVDFLAISFVKSAEVIKHLKSYIAARSRGSDISIIAKIESLDSLKNLEEIIL 360

Query: 422 ASDGAMVARGDLGAQIPLEQVPSVQQKVVQLCRRLNKPVIVASQLLESMIEYPTPTRAEV 481
           ASDGAMVARGDLGAQIPLEQVPSVQQKVVQLCR+LNKPVIVASQLLESMIEYPTPTRAEV
Sbjct: 361 ASDGAMVARGDLGAQIPLEQVPSVQQKVVQLCRQLNKPVIVASQLLESMIEYPTPTRAEV 420

Query: 482 ADVSEAVRQRADALMLSGESAMGQFPEKALTVLRSVSLRIEKWWRDEKRHEAMELPEVGS 541
           ADVSEAVRQR+DALMLSGESAMGQ+P+KAL VLRSVSLRIEKWWRDEKRHE MELPEVGS
Sbjct: 421 ADVSEAVRQRSDALMLSGESAMGQYPDKALAVLRSVSLRIEKWWRDEKRHEPMELPEVGS 480

Query: 542 SYSDSILEEICNSTAKMANNLEVDAIFVYTKTGHMASLLSRCRPDCPIFAFTSTTSVRRR 601
           S+SDSILEEICNS AKMANNLEVDAIFVYT +GHMASLLSRCRPDCPIFAFTSTTSVRRR
Sbjct: 481 SFSDSILEEICNSAAKMANNLEVDAIFVYTTSGHMASLLSRCRPDCPIFAFTSTTSVRRR 540

Query: 602 LNLQWGLIPFRLSFSDDMENNLNKTFMLLKARNLIKSGDLVLAVSDMLQSIQVMNVP 659
           LNLQWGLIPFRLSFSDDMENNLNKTF+LLKARNLIKSGDLV+AVSDMLQSIQVMNVP
Sbjct: 541 LNLQWGLIPFRLSFSDDMENNLNKTFLLLKARNLIKSGDLVIAVSDMLQSIQVMNVP 591

BLAST of Carg15878 vs. ExPASy TrEMBL
Match: A0A6J1L0W9 (Pyruvate kinase OS=Cucurbita maxima OX=3661 GN=LOC111499365 PE=3 SV=1)

HSP 1 Score: 1055.0 bits (2727), Expect = 1.2e-304
Identity = 548/597 (91.79%), Postives = 572/597 (95.81%), Query Frame = 0

Query: 62  MAQSLQLFTSSTTRSPPISLPKHSLSKTPSSSTAAFRLPVSFNKFSIRASSSPDLNPLSS 121
           M QSLQ+FTS+ TRSP ISL KHSLSK PS STAAFR P+SF K SIRASSSPD NPLSS
Sbjct: 1   MTQSLQIFTSA-TRSPGISLSKHSLSK-PSPSTAAFRFPLSFTKSSIRASSSPDSNPLSS 60

Query: 122 ASTSQVLVSENGSSSGGGFVSTSATTREFVPAVSDGSSIDVDTVTEAELKENGFRSTRRT 181
           A TSQVLVSENGS+ GGG +S S+ TREFVP +SD ++I+VDTVTEAELKENGFRSTRRT
Sbjct: 61  AFTSQVLVSENGSTGGGGALS-SSVTREFVPVMSDSTAIEVDTVTEAELKENGFRSTRRT 120

Query: 182 KLVCTIGPVTCGFEQLEALAVGGMNVARINMCHGTREWHRMVIERVRRLNEEKGYAVAIM 241
           KLVCTIGPVTCGFEQLEALAVGGMNVARINMCHGTR+WHRMVIERVRRLNEEKGYAVAIM
Sbjct: 121 KLVCTIGPVTCGFEQLEALAVGGMNVARINMCHGTRDWHRMVIERVRRLNEEKGYAVAIM 180

Query: 242 MDTEGSEIHMGDLGGASSAKAEDGEIWTFSVRAFDSTLPERTINVNYEGFAEDVRVGDDL 301
           MDTEGSEIHMGDLGGASSAKAEDGEIWTFSVRAFDSTLPERTINVNYEGFAEDVRVGDDL
Sbjct: 181 MDTEGSEIHMGDLGGASSAKAEDGEIWTFSVRAFDSTLPERTINVNYEGFAEDVRVGDDL 240

Query: 302 LVDGGMVRFEVIEKIGPDVKCLCTDPGLLLPRANLTFWRDGHLVRERNAMLPTISSKDWL 361
           LVDGGMVRFEVIEKIGPDVKCLCTDPGLLLPRANLTFWRDGHLVRERNAMLPTISSKDWL
Sbjct: 241 LVDGGMVRFEVIEKIGPDVKCLCTDPGLLLPRANLTFWRDGHLVRERNAMLPTISSKDWL 300

Query: 362 DIDFGISEGVDFLAISFVKSAEVIKHLKSYIAARSRGSDISIIAKIESLDSLKNLEEIIV 421
           DIDFGI+EGVDFLAISFVKSAEVIKHLKSYIAARSRGSDISI+AKIESLDSL NLEEII+
Sbjct: 301 DIDFGIAEGVDFLAISFVKSAEVIKHLKSYIAARSRGSDISILAKIESLDSLNNLEEIIL 360

Query: 422 ASDGAMVARGDLGAQIPLEQVPSVQQKVVQLCRRLNKPVIVASQLLESMIEYPTPTRAEV 481
           ASDGAMVARGDLGAQIPLEQVP VQQKVVQLCR+LNKPVIVASQLLESMIEYPTPTRAEV
Sbjct: 361 ASDGAMVARGDLGAQIPLEQVPLVQQKVVQLCRQLNKPVIVASQLLESMIEYPTPTRAEV 420

Query: 482 ADVSEAVRQRADALMLSGESAMGQFPEKALTVLRSVSLRIEKWWRDEKRHEAMELPEVGS 541
           AD+SEAVRQR+DALMLSGESAMGQ+P+KAL VLR+VSLRIEKWWR+EKRHE MELPEVGS
Sbjct: 421 ADISEAVRQRSDALMLSGESAMGQYPDKALAVLRNVSLRIEKWWREEKRHEPMELPEVGS 480

Query: 542 SYSDSILEEICNSTAKMANNLEVDAIFVYTKTGHMASLLSRCRPDCPIFAFTSTTSVRRR 601
           S SDSILEEICNS AKMANNLEVDAIFVYTKTGHMASLLSRCRPDCPIFAFT TTSVRRR
Sbjct: 481 SSSDSILEEICNSAAKMANNLEVDAIFVYTKTGHMASLLSRCRPDCPIFAFTETTSVRRR 540

Query: 602 LNLQWGLIPFRLSFSDDMENNLNKTFMLLKARNLIKSGDLVLAVSDMLQSIQVMNVP 659
           LNLQWGLIPFR+SFSDDMENNLNK F+LLKARNLIKSGDLV+AVSDMLQSIQVMNVP
Sbjct: 541 LNLQWGLIPFRMSFSDDMENNLNKIFLLLKARNLIKSGDLVIAVSDMLQSIQVMNVP 594

BLAST of Carg15878 vs. ExPASy TrEMBL
Match: A0A5A7UTK2 (Pyruvate kinase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold216G001510 PE=3 SV=1)

HSP 1 Score: 1054.3 bits (2725), Expect = 2.1e-304
Identity = 553/597 (92.63%), Postives = 571/597 (95.64%), Query Frame = 0

Query: 62  MAQSLQLFTSSTTRSPPISLPKHSLSKTPSSSTAAFRLPVSFNKFSIRASSSPDLNPLSS 121
           M QSLQLFTSS      ISLPKHS+SK P SSTAAFR P SF+K SIRASSS DLNPLSS
Sbjct: 1   MTQSLQLFTSSA-----ISLPKHSISK-PFSSTAAFRFPFSFSKSSIRASSS-DLNPLSS 60

Query: 122 ASTSQVLVSENGSSSGGGFVSTSATTREFVPAVSDGSSIDVDTVTEAELKENGFRSTRRT 181
           +STSQVLVSENGSSSGGG VS+SA TREFV   SD +SIDVD VTEAELKENGFRSTRRT
Sbjct: 61  SSTSQVLVSENGSSSGGGVVSSSA-TREFVSVASDSTSIDVDAVTEAELKENGFRSTRRT 120

Query: 182 KLVCTIGPVTCGFEQLEALAVGGMNVARINMCHGTREWHRMVIERVRRLNEEKGYAVAIM 241
           KLVCTIGP +CGFEQLEALAVGGMNVARINMCHGTR+WHR VIERVRRLN+EKGYAVAIM
Sbjct: 121 KLVCTIGPASCGFEQLEALAVGGMNVARINMCHGTRDWHRTVIERVRRLNDEKGYAVAIM 180

Query: 242 MDTEGSEIHMGDLGGASSAKAEDGEIWTFSVRAFDSTLPERTINVNYEGFAEDVRVGDDL 301
           MDTEGSEIHMGDLGGASSAKAEDGEIWTFSVRAFDSTLPERTINVNYEGFAEDVRVGDDL
Sbjct: 181 MDTEGSEIHMGDLGGASSAKAEDGEIWTFSVRAFDSTLPERTINVNYEGFAEDVRVGDDL 240

Query: 302 LVDGGMVRFEVIEKIGPDVKCLCTDPGLLLPRANLTFWRDGHLVRERNAMLPTISSKDWL 361
           LVDGGMVRFEVIEKIGPDVKCLCTDPGLLLPRANLTFWRDGHLVRERNAMLPTISSKDWL
Sbjct: 241 LVDGGMVRFEVIEKIGPDVKCLCTDPGLLLPRANLTFWRDGHLVRERNAMLPTISSKDWL 300

Query: 362 DIDFGISEGVDFLAISFVKSAEVIKHLKSYIAARSRGSDISIIAKIESLDSLKNLEEIIV 421
           DIDFGI+EGVDFLAISFVKSAEVIKHLKSYIAARSRGSDISIIAKIESLDSLKNLEEII+
Sbjct: 301 DIDFGIAEGVDFLAISFVKSAEVIKHLKSYIAARSRGSDISIIAKIESLDSLKNLEEIIL 360

Query: 422 ASDGAMVARGDLGAQIPLEQVPSVQQKVVQLCRRLNKPVIVASQLLESMIEYPTPTRAEV 481
           ASDGAMVARGDLGAQIPLEQVPSVQQKVVQLCR+LNKPVIVASQLLESMIEYPTPTRAEV
Sbjct: 361 ASDGAMVARGDLGAQIPLEQVPSVQQKVVQLCRQLNKPVIVASQLLESMIEYPTPTRAEV 420

Query: 482 ADVSEAVRQRADALMLSGESAMGQFPEKALTVLRSVSLRIEKWWRDEKRHEAMELPEVGS 541
           ADVSEAVRQR+DALMLSGESAMGQ+P+KAL VLRSVSLRIEKWWRDEKRHE MELPEVGS
Sbjct: 421 ADVSEAVRQRSDALMLSGESAMGQYPDKALAVLRSVSLRIEKWWRDEKRHEPMELPEVGS 480

Query: 542 SYSDSILEEICNSTAKMANNLEVDAIFVYTKTGHMASLLSRCRPDCPIFAFTSTTSVRRR 601
           S+SDSILEEICNS AKMANNLEVDAIFVYT +GHMASLLSRCRPDCPIFAFTSTTSVRRR
Sbjct: 481 SFSDSILEEICNSAAKMANNLEVDAIFVYTTSGHMASLLSRCRPDCPIFAFTSTTSVRRR 540

Query: 602 LNLQWGLIPFRLSFSDDMENNLNKTFMLLKARNLIKSGDLVLAVSDMLQSIQVMNVP 659
           LNLQWGLIPFRLSFSDDMENNLNKTF+LLKARNLIKSGDLV+AVSDMLQSIQVMNVP
Sbjct: 541 LNLQWGLIPFRLSFSDDMENNLNKTFLLLKARNLIKSGDLVIAVSDMLQSIQVMNVP 589

BLAST of Carg15878 vs. TAIR 10
Match: AT3G22960.1 (Pyruvate kinase family protein )

HSP 1 Score: 912.9 bits (2358), Expect = 1.5e-265
Identity = 474/602 (78.74%), Postives = 531/602 (88.21%), Query Frame = 0

Query: 62  MAQSLQLFTSSTTRSPPISLPKHSLSKTPSSSTAAFRLPVSFNKF-SIRASS----SPDL 121
           M+QS+Q  T S T  P +    HS    P SS +  R P++  K+ SIRASS    SPDL
Sbjct: 1   MSQSIQFSTPSHT--PHLLHLPHSQFNRPLSSISFRRFPLTTIKYTSIRASSSSSPSPDL 60

Query: 122 NPLSSASTSQVLVSENGSSSGGGFVSTSATTREFVPAVSDGSSIDVDTVTEAELKENGFR 181
           +  SS+S+SQVL+S NG+    G V +   +       +D S I+VDTVTEAELKENGFR
Sbjct: 61  DSSSSSSSSQVLLSPNGT----GAVKSDERSVVATAVTTDTSGIEVDTVTEAELKENGFR 120

Query: 182 STRRTKLVCTIGPVTCGFEQLEALAVGGMNVARINMCHGTREWHRMVIERVRRLNEEKGY 241
           STRRTKL+CTIGP TCGFEQLEALAVGGMNVAR+NMCHGTR+WHR VI  VRRLNEEKG+
Sbjct: 121 STRRTKLICTIGPATCGFEQLEALAVGGMNVARLNMCHGTRDWHRGVIRSVRRLNEEKGF 180

Query: 242 AVAIMMDTEGSEIHMGDLGGASSAKAEDGEIWTFSVRAFDSTLPERTINVNYEGFAEDVR 301
           AVAIMMDTEGSEIHMGDLGG +SAKAEDGE+WTF+VRAFDS+ PERTI+V+Y+GFAEDVR
Sbjct: 181 AVAIMMDTEGSEIHMGDLGGEASAKAEDGEVWTFTVRAFDSSRPERTISVSYDGFAEDVR 240

Query: 302 VGDDLLVDGGMVRFEVIEKIGPDVKCLCTDPGLLLPRANLTFWRDGHLVRERNAMLPTIS 361
           VGD+LLVDGGMVRFEVIEKIGPDVKCLCTDPGLLLPRANLTFWRDG LVRERNAMLPTIS
Sbjct: 241 VGDELLVDGGMVRFEVIEKIGPDVKCLCTDPGLLLPRANLTFWRDGSLVRERNAMLPTIS 300

Query: 362 SKDWLDIDFGISEGVDFLAISFVKSAEVIKHLKSYIAARSRGSDISIIAKIESLDSLKNL 421
           SKDWLDIDFGI+EGVDF+A+SFVKSAEVI HLKSY+AARSRG +I +IAKIES+DSL NL
Sbjct: 301 SKDWLDIDFGIAEGVDFIAVSFVKSAEVINHLKSYLAARSRGGEIGVIAKIESIDSLTNL 360

Query: 422 EEIIVASDGAMVARGDLGAQIPLEQVPSVQQKVVQLCRRLNKPVIVASQLLESMIEYPTP 481
           EEII+ASDGAMVARGDLGAQIPLEQVP+ QQ++VQ+CR LNKPVIVASQLLESMIEYPTP
Sbjct: 361 EEIILASDGAMVARGDLGAQIPLEQVPAAQQRIVQVCRALNKPVIVASQLLESMIEYPTP 420

Query: 482 TRAEVADVSEAVRQRADALMLSGESAMGQFPEKALTVLRSVSLRIEKWWRDEKRHEAMEL 541
           TRAEVADVSEAVRQR+DALMLSGESAMGQFP+KALTVLR+VSLRIE+WWR+EKRHE++ L
Sbjct: 421 TRAEVADVSEAVRQRSDALMLSGESAMGQFPDKALTVLRTVSLRIERWWREEKRHESVPL 480

Query: 542 PEVGSSYSDSILEEICNSTAKMANNLEVDAIFVYTKTGHMASLLSRCRPDCPIFAFTSTT 601
             +GSS+SD I EEICNS AKMANNL VDA+FVYT +GHMASL+SRCRPDCPIFAFT+TT
Sbjct: 481 QAIGSSFSDKISEEICNSAAKMANNLGVDAVFVYTTSGHMASLVSRCRPDCPIFAFTTTT 540

Query: 602 SVRRRLNLQWGLIPFRLSFSDDMENNLNKTFMLLKARNLIKSGDLVLAVSDMLQSIQVMN 659
           SVRRRLNLQWGLIPFRLSFSDDME+NLNKTF LLK+R +IKSGDLV+AVSDMLQSIQVMN
Sbjct: 541 SVRRRLNLQWGLIPFRLSFSDDMESNLNKTFSLLKSRGMIKSGDLVIAVSDMLQSIQVMN 596

BLAST of Carg15878 vs. TAIR 10
Match: AT5G52920.1 (plastidic pyruvate kinase beta subunit 1 )

HSP 1 Score: 364.8 bits (935), Expect = 1.5e-100
Identity = 208/475 (43.79%), Postives = 291/475 (61.26%), Query Frame = 0

Query: 179 RRTKLVCTIGPVTCGFEQLEALAVGGMNVARINMCHGTREWHRMVIERVRRLN-EEKGYA 238
           R+TK+VCT+GP T   E +  LA  GMNVAR+NM HG    H+ VI+ V+  N + K   
Sbjct: 110 RKTKIVCTVGPSTNTREMIWKLAEAGMNVARMNMSHGDHASHKKVIDLVKEYNAQTKDNT 169

Query: 239 VAIMMDTEGSEIHMGDLGGASSAKAEDGEIWTFSVRAFDSTLPERTINVNYEGFAEDVRV 298
           +AIM+DT+G E+  GDL        + G+ +TF++    ST     ++VNY+ F  DV  
Sbjct: 170 IAIMLDTKGPEVRSGDL--PQPIMLDPGQEFTFTIERGVST--PSCVSVNYDDFVNDVEA 229

Query: 299 GDDLLVDGGMVRFEVIEKIGPDVKCLCTDPGLLLPRANLTFWRDGHLVRERNAMLPTISS 358
           GD LLVDGGM+ F V  K    VKC   D G L  R +L        VR ++A LP+I+ 
Sbjct: 230 GDMLLVDGGMMSFMVKSKTKDSVKCEVVDGGELKSRRHLN-------VRGKSATLPSITE 289

Query: 359 KDWLDIDFGISEGVDFLAISFVKSAEVIKHLKSYIAARSRGSDISIIAKIESLDSLKNLE 418
           KDW DI FG+   VDF A+SFVK A+V+  LK Y+  ++ G+DI +I KIES DS+ NL 
Sbjct: 290 KDWEDIKFGVENKVDFYAVSFVKDAQVVHELKKYL--QNSGADIHVIVKIESADSIPNLH 349

Query: 419 EIIVASDGAMVARGDLGAQIPLEQVPSVQQKVVQLCRRLNKPVIVASQLLESMIEYPTPT 478
            II ASDGAMVARGDLGA++P+E+VP +Q++++ LCR + K VIVA+ +LESMI +PTPT
Sbjct: 350 SIITASDGAMVARGDLGAELPIEEVPILQEEIINLCRSMGKAVIVATNMLESMIVHPTPT 409

Query: 479 RAEVADVSEAVRQRADALMLSGESAMGQFPEKALTVLRSVSLRIEKWWRDEKRHEAMELP 538
           RAEV+D++ AVR+ ADA+MLSGE+A G+FP KA  V+ +V+LR E      +       P
Sbjct: 410 RAEVSDIAIAVREGADAVMLSGETAHGKFPLKAAGVMHTVALRTEATITSGEMP-----P 469

Query: 539 EVGSSYSDSILEEICNSTAKMANNLEVDAIFVYTKTGHMASLLSRCRPDCPIFAFTSTTS 598
            +G ++ + + E        M+N L    + V+T+TG MA LLS  RP   I+AFT+   
Sbjct: 470 NLGQAFKNHMSEMFAYHATMMSNTLGTSTV-VFTRTGFMAILLSHYRPSGTIYAFTNEKK 529

Query: 599 VRRRLNLQWGLIPFRLSFSDDMENNLNKTFMLLKARNLIKSGDLVLAVSDMLQSI 653
           +++RL L  G+ P  + F+DD E         L  + ++K G+ +  V    Q I
Sbjct: 530 IQQRLALYQGVCPIYMEFTDDAEETFANALATLLKQGMVKKGEEIAIVQSGTQPI 565

BLAST of Carg15878 vs. TAIR 10
Match: AT1G32440.1 (plastidial pyruvate kinase 3 )

HSP 1 Score: 359.0 bits (920), Expect = 8.2e-99
Identity = 227/552 (41.12%), Postives = 327/552 (59.24%), Query Frame = 0

Query: 107 SIRASSSPDLNPLSSASTSQVLVSENGSSSGGGFVSTSATTREFVPAVSDGSSIDVDTVT 166
           +I  S SP    L  A      +S    S     +  S  +R+   A ++  + DV  + 
Sbjct: 23  NIGVSLSPLRRTLIGAGVRSTSISLRQCSLSVRSIKISEDSRK-PKAYAENGAFDVGVLD 82

Query: 167 E-----AELKENGFRSTRRTKLVCTIGPVTCGFEQLEALAVGGMNVARINMCHGTREWHR 226
                 A+ + +   S R+TK+VCTIGP +   E +  LA  GMNVAR+NM HG    H+
Sbjct: 83  SSSYRLADSRTSSNDSRRKTKIVCTIGPSSSSREMIWKLAEAGMNVARLNMSHGDHASHQ 142

Query: 227 MVIERVRRLNE-EKGYAVAIMMDTEGSEIHMGDLGGASSAKAEDGEIWTFSVRAFDSTLP 286
           + I+ V+  N      A+AIM+DT+G E+  GD+        E+G+ + F+++   S   
Sbjct: 143 ITIDLVKEYNSLFVDKAIAIMLDTKGPEVRSGDV--PQPIFLEEGQEFNFTIKRGVSL-- 202

Query: 287 ERTINVNYEGFAEDVRVGDDLLVDGGMVRFEVIEKIGPDVKCLCTDPGLLLPRANLTFWR 346
           + T++VNY+ F  DV VGD LLVDGGM+   V  K    VKC+  D G L  R +L    
Sbjct: 203 KDTVSVNYDDFVNDVEVGDILLVDGGMMSLAVKSKTSDLVKCVVIDGGELQSRRHLN--- 262

Query: 347 DGHLVRERNAMLPTISSKDWLDIDFGISEGVDFLAISFVKSAEVIKHLKSYIAARSRGSD 406
               VR ++A LP+I+ KDW DI FG+   VDF A+SFVK A+V+  LK+Y+  ++  +D
Sbjct: 263 ----VRGKSATLPSITDKDWEDIKFGVDNQVDFYAVSFVKDAKVVHELKNYL--KTCSAD 322

Query: 407 ISIIAKIESLDSLKNLEEIIVASDGAMVARGDLGAQIPLEQVPSVQQKVVQLCRRLNKPV 466
           IS+I KIES DS+KNL  II A DGAMVARGDLGA++P+E+VP +Q+++++ CR ++KPV
Sbjct: 323 ISVIVKIESADSIKNLPSIISACDGAMVARGDLGAELPIEEVPLLQEEIIRRCRSIHKPV 382

Query: 467 IVASQLLESMIEYPTPTRAEVADVSEAVRQRADALMLSGESAMGQFPEKALTVLRSVSLR 526
           IVA+ +LESMI +PTPTRAEV+D++ AVR+ ADA+MLSGE+A G+FP KA+ V+ +V+LR
Sbjct: 383 IVATNMLESMINHPTPTRAEVSDIAIAVREGADAIMLSGETAHGKFPLKAVNVMHTVALR 442

Query: 527 IEKWWRDEKRHEAMELPEVGSSYSDSILEEICNSTAKMANNLEVDAIFVYTKTGHMASLL 586
            E       R  A       ++Y   + +      + MAN L    + V+T+TG MA LL
Sbjct: 443 TEA--SLPVRTSASRT----TAYKGHMGQMFAFHASIMANTLS-SPLIVFTRTGSMAVLL 502

Query: 587 SRCRPDCPIFAFTSTTSVRRRLNLQWGLIPFRLSFSDDMENNLNKTFMLLKARNLIKSGD 646
           S  RP   IFAFT+   + +RL L  G++P  + FSDD E+   ++  LL+  N++K G 
Sbjct: 503 SHYRPSATIFAFTNQRRIMQRLALYQGVMPIYMEFSDDAEDTYARSLKLLQDENMLKEGQ 553

Query: 647 LVLAVSDMLQSI 653
            V  V    Q I
Sbjct: 563 HVTLVQSGSQPI 553

BLAST of Carg15878 vs. TAIR 10
Match: AT5G63680.1 (Pyruvate kinase family protein )

HSP 1 Score: 235.7 bits (600), Expect = 1.0e-61
Identity = 160/510 (31.37%), Postives = 265/510 (51.96%), Query Frame = 0

Query: 158 SSIDVDTVTEAELKENGFRSTRRTKLVCTIGPVTCGFEQLEALAVGGMNVARINMCHGTR 217
           S+ID++ + + EL  +G   T +TK+VCT+GP +     +E L   GMNVAR N  HG+ 
Sbjct: 2   SNIDIEGILK-ELPNDG--RTPKTKIVCTLGPASRSVTMIEKLLKAGMNVARFNFSHGSH 61

Query: 218 EWHRMVIERVRRLNEEKGYAVAIMMDTEGSEIHMGDLGGASSAKAEDGEIWTFSVRAFDS 277
           E+H+  ++ +R   +  G   A+M+DT+G EI  G L   +  + ++G+  T +   +D 
Sbjct: 62  EYHQETLDNLRTAMQNTGILAAVMLDTKGPEIRTGFLKDGNPIQLKEGQEITITT-DYDI 121

Query: 278 TLPERTINVNYEGFAEDVRVGDDLLVDGGMVRFEVI--EKIGPDVKCLCTDPGLLLPRAN 337
              E+TI+++Y+    DV+ G+ +L   G +   V+  +     V C C +  +L  R N
Sbjct: 122 KGDEKTISMSYKKLPVDVKPGNTILCADGSISLAVVSCDPNAGTVICRCENTAMLGERKN 181

Query: 338 LTFWRDGHLVRERNAMLPTISSKDWLDI-DFGISEGVDFLAISFVKSAEVIKHLKSYIAA 397
           +     G +V      LPT++ KD  DI  +G+   +D +A+SFV+    + +++  + +
Sbjct: 182 VNL--PGVVVD-----LPTLTDKDVEDILKWGVPNNIDMIALSFVRKGSDLVNVRKVLGS 241

Query: 398 RSRGSDISIIAKIESLDSLKNLEEIIVASDGAMVARGDLGAQIPLEQVPSVQQKVVQLCR 457
            S+   I +++K+E+ + + N +EI+  +D  MVARGDLG +IP+E++   Q+ ++  C 
Sbjct: 242 HSK--SIMLMSKVENQEGVLNFDEILRETDAFMVARGDLGMEIPIEKIFLAQKMMIYKCN 301

Query: 458 RLNKPVIVASQLLESMIEYPTPTRAEVADVSEAVRQRADALMLSGESAMGQFPEKALTVL 517
              KPV+ A+Q+LESMI+ P PTRAE  DV+ AV    D +MLSGESA G +PE A+  +
Sbjct: 302 LAGKPVVTATQMLESMIKSPRPTRAEATDVANAVLDGTDCVMLSGESAAGAYPEIAVKTM 361

Query: 518 RSVSLRIEKWWRDEKRHEAMELPEVGSSYSDSILEEICNSTAKMANNLEVDAIFVYTKTG 577
             + +  E         + M      +    S LE + +S  + AN  +   I V T+ G
Sbjct: 362 AKICIEAESSLDYNTIFKEMIR---ATPLPMSTLESLASSAVRTANKAKAKLIIVLTRGG 421

Query: 578 HMASLLSRCRPDCPIFA-----FTSTT--------SVRRRLNLQWGLIPF------RLSF 637
             A L+++ RP  PI +     FTS T        S  R   +  GLIP       + + 
Sbjct: 422 TTAKLVAKYRPAVPILSVVVPVFTSDTFNWSCSDESPARHSLIYRGLIPVLGEGSAKATD 481

Query: 638 SDDMENNLNKTFMLLKARNLIKSGDLVLAV 646
           S+  E  +         + L   GD V+A+
Sbjct: 482 SESTEEIIESALKSATEKGLCNHGDAVVAL 495

BLAST of Carg15878 vs. TAIR 10
Match: AT3G55650.1 (Pyruvate kinase family protein )

HSP 1 Score: 228.0 bits (580), Expect = 2.2e-59
Identity = 156/493 (31.64%), Postives = 252/493 (51.12%), Query Frame = 0

Query: 180 RTKLVCTIGPVTCGFEQLEALAVGGMNVARINMCHGTREWHRMVIERVRRLNEEKGYAVA 239
           +TK++CT+GPV+   E +E L   GMNVAR N  HG+  +H+  ++ +R   +  G   A
Sbjct: 17  KTKIICTLGPVSRSVEMIEKLLKAGMNVARFNFSHGSHSYHQETLDNLRTAMDNTGILSA 76

Query: 240 IMMDTEGSEIHMGDLGGASSAKAEDGEIWTFSVRAFDSTLPERTINVNYEGFAEDVRVGD 299
           +M+DT+G EI  G L      +   G+  T S+  +        I+++Y+  AEDV+ GD
Sbjct: 77  VMLDTKGPEIRTGFLKEGKPIQLNQGQEITISI-DYMIEGDSNVISMSYKKLAEDVKPGD 136

Query: 300 DLLVDGGMVRFEVI--EKIGPDVKCLCTDPGLLLPRANLTFWRDGHLVRERNAMLPTISS 359
            +L   G +   V+  +K    V+C C +  +L  R N+     G +V      LPT++ 
Sbjct: 137 VILCSDGTISLTVLSCDKSFGLVRCRCENSAILGERKNVNL--PGIVVD-----LPTLTE 196

Query: 360 KDWLD-IDFGISEGVDFLAISFVKSAEVIKHLKSYIAARSRGSDISIIAKIESLDSLKNL 419
           KD  D I +G+   +D +A+SFV+    +  ++  +   S+  +I +++K+E+ + + N 
Sbjct: 197 KDKEDIIQWGVPNKIDIIALSFVRKGSDLTEVRRLLGEHSK--NIMLMSKVENQEGVMNC 256

Query: 420 EEIIVASDGAMVARGDLGAQIPLEQVPSVQQKVVQLCRRLNKPVIVASQLLESMIEYPTP 479
           E+I+  SD  MVARGDLG +IP+E++   Q+ ++++   L KPV+ A+Q+LESM   P P
Sbjct: 257 EKILENSDAFMVARGDLGMEIPIEKMFLAQKTMIKMANALGKPVVTATQMLESMTVSPRP 316

Query: 480 TRAEVADVSEAVRQRADALMLSGESAMGQFPEKALTVLRSVSLRIEKWWRDEKRHEAMEL 539
           TRAE  DV+ AV    D +MLSGE+A G  PE A+  +     RI K   D   ++ +  
Sbjct: 317 TRAEATDVANAVLDGTDCVMLSGETAAGAHPEAAVLTMS----RICKEAEDFIDYDILHK 376

Query: 540 PEVGS-SYSDSILEEICNSTAKMANNLEVDAIFVYTKTGHMASLLSRCRPDCPIFAF--- 599
             +G  S   S +E +  S    A ++   AI V TK G+ A L+++ RP  PI +    
Sbjct: 377 KTLGMVSLPLSPIESLAASVVSTAQSVFASAIVVLTKGGYTAELVAKYRPSVPILSVIVP 436

Query: 600 -------------TSTTSVRRRLNLQWGLIPF-------RLSFSDDMENNLNKTFMLLKA 646
                         S     RR  +   +IP        R S  D  E  +N      K 
Sbjct: 437 EIAQGNDMEMSCSDSVAHAARRGLIYRRIIPVVATGSSARDSNKDATEEMINLAIGFAKT 495

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
KAG7026211.10.0e+00100.00hypothetical protein SDJN02_12710, partial [Cucurbita argyrosperma subsp. argyro... [more]
KAG6593869.10.0e+0099.85hypothetical protein SDJN03_13345, partial [Cucurbita argyrosperma subsp. sorori... [more]
XP_023000477.10.0e+0099.66pyruvate kinase isozyme A, chloroplastic-like [Cucurbita maxima][more]
XP_023514423.10.0e+0099.16pyruvate kinase isozyme A, chloroplastic-like [Cucurbita pepo subsp. pepo][more]
XP_022930416.10.0e+0099.33pyruvate kinase isozyme A, chloroplastic-like [Cucurbita moschata][more]
Match NameE-valueIdentityDescription
Q431173.4e-26780.80Pyruvate kinase isozyme A, chloroplastic OS=Ricinus communis OX=3988 PE=1 SV=1[more]
Q405451.9e-26579.90Pyruvate kinase isozyme A, chloroplastic OS=Nicotiana tabacum OX=4097 PE=2 SV=1[more]
Q9LIK02.1e-26478.74Plastidial pyruvate kinase 1, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=P... [more]
Q405465.9e-10244.44Pyruvate kinase isozyme G, chloroplastic OS=Nicotiana tabacum OX=4097 PE=2 SV=1[more]
Q9FLW92.1e-9943.79Plastidial pyruvate kinase 2 OS=Arabidopsis thaliana OX=3702 GN=PKP2 PE=1 SV=1[more]
Match NameE-valueIdentityDescription
A0A6J1KDQ90.0e+0099.66Pyruvate kinase OS=Cucurbita maxima OX=3661 GN=LOC111494735 PE=3 SV=1[more]
A0A6J1EQW00.0e+0099.33Pyruvate kinase OS=Cucurbita moschata OX=3662 GN=LOC111436871 PE=3 SV=1[more]
A0A0A0LKS31.1e-30592.29Pyruvate kinase OS=Cucumis sativus OX=3659 GN=Csa_2G215480 PE=3 SV=1[more]
A0A6J1L0W91.2e-30491.79Pyruvate kinase OS=Cucurbita maxima OX=3661 GN=LOC111499365 PE=3 SV=1[more]
A0A5A7UTK22.1e-30492.63Pyruvate kinase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold216G0015... [more]
Match NameE-valueIdentityDescription
AT3G22960.11.5e-26578.74Pyruvate kinase family protein [more]
AT5G52920.11.5e-10043.79plastidic pyruvate kinase beta subunit 1 [more]
AT1G32440.18.2e-9941.12plastidial pyruvate kinase 3 [more]
AT5G63680.11.0e-6131.37Pyruvate kinase family protein [more]
AT3G55650.12.2e-5931.64Pyruvate kinase family protein [more]
InterPro
Analysis Name: InterPro Annotations of Silver-seed gourd (SMH-JMG-627) v2
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR001697Pyruvate kinasePRINTSPR01050PYRUVTKNASEcoord: 480..498
score: 54.41
coord: 371..385
score: 41.74
coord: 236..252
score: 45.07
coord: 499..515
score: 38.73
coord: 403..429
score: 51.79
coord: 455..479
score: 60.98
coord: 430..454
score: 45.91
IPR001697Pyruvate kinaseTIGRFAMTIGR01064TIGR01064coord: 179..647
e-value: 1.7E-127
score: 423.8
IPR001697Pyruvate kinasePANTHERPTHR11817PYRUVATE KINASEcoord: 146..658
IPR036918Pyruvate kinase, C-terminal domain superfamilyGENE3D3.40.1380.20coord: 522..648
e-value: 1.0E-149
score: 501.1
IPR036918Pyruvate kinase, C-terminal domain superfamilySUPERFAMILY52935PK C-terminal domain-likecoord: 510..656
IPR040442Pyruvate kinase-like domain superfamilyGENE3D3.20.20.60coord: 179..521
e-value: 1.0E-149
score: 501.1
IPR015795Pyruvate kinase, C-terminalPFAMPF02887PK_Ccoord: 549..648
e-value: 1.1E-21
score: 77.3
IPR015806Pyruvate kinase, insert domain superfamilyGENE3D2.40.33.10coord: 247..353
e-value: 1.0E-149
score: 501.1
IPR015793Pyruvate kinase, barrelPFAMPF00224PKcoord: 179..524
e-value: 2.7E-91
score: 306.1
NoneNo IPR availablePANTHERPTHR11817:SF14PLASTIDIAL PYRUVATE KINASE 1, CHLOROPLASTICcoord: 146..658
IPR018209Pyruvate kinase, active sitePROSITEPS00110PYRUVATE_KINASEcoord: 401..413
IPR011037Pyruvate kinase-like, insert domain superfamilySUPERFAMILY50800PK beta-barrel domain-likecoord: 248..339
IPR015813Pyruvate/Phosphoenolpyruvate kinase-like domain superfamilySUPERFAMILY51621Phosphoenolpyruvate/pyruvate domaincoord: 163..525

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Carg15878-RACarg15878-RAmRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0006096 glycolytic process
biological_process GO:0006629 lipid metabolic process
biological_process GO:0010431 seed maturation
cellular_component GO:0009570 chloroplast stroma
molecular_function GO:0005524 ATP binding
molecular_function GO:0016301 kinase activity
molecular_function GO:0000287 magnesium ion binding
molecular_function GO:0030955 potassium ion binding
molecular_function GO:0004743 pyruvate kinase activity
molecular_function GO:0003824 catalytic activity