Carg15690 (gene) Silver-seed gourd (SMH-JMG-627) v2

Overview


Name	Carg15690
Type	gene
Organism	Cucurbita argyrosperma subsp. argyrosperma cv. SMH-JMG-627 (Silver-seed gourd (SMH-JMG-627) v2)
Description	taxadiene 5-alpha hydroxylase
Location	Carg_Chr19: 8882770 .. 8885267 (+)
RNA-Seq Expression	Carg15690
Synteny	Carg15690

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptidethree_prime_UTR

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGGTTCAAGAATTTAGCTGCTTTCTGTTGGCAACAATGTTCTTGTTTTTATCGGCAATCTTAATAATATCCATTGTTTTTGTGTTTAGAAGCATAAAGGGAAGCAAAAAGGTACCGCCTGGGCAAATGGGTCTGCCCTGGATTGGCGAAACCATGGCTTTCTACAGAGCCCAACGTAAAAACAGATTGTTCGAAGAGTTTGTTGGGCCTCGAGTGGCTAAATATGGCAAAATCTTCAAAACAAGCTTGATGGGGTCGCCGACGGTGGTCGTAAATGGAGCTGAAGCCAACAGATTTTTCTTGTCCAATGAGTTTAAGTTGGTGGTAAGCTCATGGCCGTCGGCCTCTGTTCAGCTGATGGGCGAAGAATGCATCATGCAGAAGCAAGGTGAAAGCCACCGATGTGCGCGTGGCCTTATTGCCGCAAGCCTTGCCAGCTCGAGTCTCGAAGCAATGATGCCTAAATTGTGCACTACAATTCAGCTTTATTTGGACACCAAATGGGTTGGCCACGACATAATTAGTCTTTACCACTCGGCAAAAGTATTGACGTTTACCATAGTGTTTGAGTGTTTGATGGGTATCAAAGTGGAGCCACGCATGTTGACGACATTCGAGAGGGTTTTGGAAGGAGTTTTTGCACCACCGTTCAGGTAGTTATTCAACTATACCCCTTGATTCAAAAGTTTAGGCTGATTGATAGGTTATGTTAAATTTAAATCTTGTTTGATAGCGATTTCATTTTTAAAATTTCCTTCCTATGACATTTATCTTTCTCATTTTTAAAATTTCCTTCCTATGACATTTATCTTTCTTACCTTTTTGATTTCAAACTTGACTCGATTTTTTAAAGTATTAGTAGAATGTGAATAGAGAAATTTATATATGAAATTTGTGTTTATAAACTTGATTTTCAAATACCAAAGATCAAATACAAATGGACATCAAATGGAACTCGAAGTTTTAATTATTTGTTCATATTCTCAACATAGTTTTCCAGGCACACGATTTTCGAGAGCGAAGAAAGCAAGGATGGAGATAGGGAAAATGCTAAGGGAGGTGGTGAGAGAGAAGAGGAGGAGAATTGAAGAAGAGCCCGAAGGAGAAGAATGTGAGTTGTTGCTTTCAAGGATGGTGAGAGCAATGATAAGAGGGGAGATCAGTGAAGTGGAAGTGGTGGACAATGTGGTTTTGCTTGTTTTTGCTGCTCATGATACAACCTCCTTCGCCATTGCCGTGACTTTCAAAATGTTGGCTCAGCAGCCAAACTGCTACTCACTTCTTCTACAGGGTAATATTTTTTTCTATAATCTTGTAAATCAATGAGTTGGGCGCCATTTCAACTTCCTTTCTATCAGAAAGAAATTTTTATTCAACCAAATATGAAAATTCTGATGGGGTTTTCAGAATATCATTTTGCTGATCTTAGTGAGAATTAAGGTGTAAATTTGATTACTTCTTAACAGAACATGCTGACATAGTGAGAAGCAAGGGACCAGGCCAGAATTTGACAATGGAAGATATAAAGAAGATGAAATATACTTGGCAGGTAGCTCGTGAGAGCATTCGACTGTTTCCTCCTATATTTGGTTCATTCAGGAAAGCCATTACAGACATTGAATACGAGGGATTTTTCATTCCCAAGGGATGGAAGGTGAAAATTTCCATCAATTCTTCTGCTTCTTCTTTACTACTGATACCCTGCAGAGTCAAATTGTAGTCCAGAAATGACCATCGTTTTACCTTGAATCATTTTGGTTGCGTGCAGGTCTTGTGGACGACGTATGGAACGCATCTCAAAGACGAGTATTTTGAAGATCCATTGAGTTTCAAGCCAAGCAGATTTGAGGAACCTGTTTCCCAGTATGTCTATGTTCCATTTGGAGGAGGGCCAAGAGCTTGTGCAGGGTACCAGCTGGCGAAATTGAACATCCTCATCTTTATACACTTGGTTGTAACTCGTTATGATTGGAGTTTAATCCACCCAGATGAGCCAATCATAATGGATCCCCTCCCTATCCCTTCTCATGGAATGCCAATTAAGATTTCTCCAAAATCAGAATCTCATCCCAATGTTCAGCAAATGTAAGCTCATAAGAGAACAAAGACGATGCACTTGAGAGGATGTAAAACCTCTAGATATCAGACATAGTTTACATAATTATGATTCTTTCCAACAGCAGACATAAAGATCTCATAAGAGGAAACCCTTTCTATATTTCACTATTGTTGCCGATCCAAGAATACAGTCTACTATATTTTAACTCAGGTGTATAACCATAAGCTAATCTAATGTACGGCCAGGGAACAAAAAGAGTGATTCTTTTCAGTTTTTCCTGATATCTACCGGGCAAAATTTTAAAAAATACTAAAGAAGTTACGGATCTTTAATGCACTGTTTAGTGTCTGGCAATAGACGATGACTAATAGGTCTTACTCCTTGCTTAAGCCAACGTTTCACCAGGAATGTTTCTAATTTCCTTACCTGCAACCACAAA

mRNA sequence

ATGGTTCAAGAATTTAGCTGCTTTCTGTTGGCAACAATGTTCTTGTTTTTATCGGCAATCTTAATAATATCCATTGTTTTTGTGTTTAGAAGCATAAAGGGAAGCAAAAAGGTACCGCCTGGGCAAATGGGTCTGCCCTGGATTGGCGAAACCATGGCTTTCTACAGAGCCCAACGTAAAAACAGATTGTTCGAAGAGTTTGTTGGGCCTCGAGTGGCTAAATATGGCAAAATCTTCAAAACAAGCTTGATGGGGTCGCCGACGGTGGTCGTAAATGGAGCTGAAGCCAACAGATTTTTCTTGTCCAATGAGTTTAAGTTGGTGGTAAGCTCATGGCCGTCGGCCTCTGTTCAGCTGATGGGCGAAGAATGCATCATGCAGAAGCAAGGTGAAAGCCACCGATGTGCGCGTGGCCTTATTGCCGCAAGCCTTGCCAGCTCGAGTCTCGAAGCAATGATGCCTAAATTGTGCACTACAATTCAGCTTTATTTGGACACCAAATGGGTTGGCCACGACATAATTAGTCTTTACCACTCGGCAAAAGTATTGACGTTTACCATAGTGTTTGAGTGTTTGATGGGTATCAAAGTGGAGCCACGCATGTTGACGACATTCGAGAGGGTTTTGGAAGGAGTTTTTGCACCACCGTTCAGTTTTCCAGGCACACGATTTTCGAGAGCGAAGAAAGCAAGGATGGAGATAGGGAAAATGCTAAGGGAGGTGGTGAGAGAGAAGAGGAGGAGAATTGAAGAAGAGCCCGAAGGAGAAGAATGTGAGTTGTTGCTTTCAAGGATGGTGAGAGCAATGATAAGAGGGGAGATCAGTGAAGTGGAAGTGGTGGACAATGTGGTTTTGCTTGTTTTTGCTGCTCATGATACAACCTCCTTCGCCATTGCCGTGACTTTCAAAATGTTGGCTCAGCAGCCAAACTGCTACTCACTTCTTCTACAGGAACATGCTGACATAGTGAGAAGCAAGGGACCAGGCCAGAATTTGACAATGGAAGATATAAAGAAGATGAAATATACTTGGCAGGTAGCTCGTGAGAGCATTCGACTGTTTCCTCCTATATTTGGTTCATTCAGGAAAGCCATTACAGACATTGAATACGAGGGATTTTTCATTCCCAAGGGATGGAAGGTCTTGTGGACGACGTATGGAACGCATCTCAAAGACGAGTATTTTGAAGATCCATTGAGTTTCAAGCCAAGCAGATTTGAGGAACCTGTTTCCCAGTATGTCTATGTTCCATTTGGAGGAGGGCCAAGAGCTTGTGCAGGGTACCAGCTGGCGAAATTGAACATCCTCATCTTTATACACTTGGTTGTAACTCGTTATGATTGGAGTTTAATCCACCCAGATGAGCCAATCATAATGGATCCCCTCCCTATCCCTTCTCATGGAATGCCAATTAAGATTTCTCCAAAATCAGAATCTCATCCCAATGTTCAGCAAATGTAAGCTCATAAGAGAACAAAGACGATGCACTTGAGAGGATGTAAAACCTCTAGATATCAGACATAGTTTACATAATTATGATTCTTTCCAACAGCAGACATAAAGATCTCATAAGAGGAAACCCTTTCTATATTTCACTATTGTTGCCGATCCAAGAATACAGTCTACTATATTTTAACTCAGGTGTATAACCATAAGCTAATCTAATGTACGGCCAGGGAACAAAAAGAGTGATTCTTTTCAGTTTTTCCTGATATCTACCGGGCAAAATTTTAAAAAATACTAAAGAAGTTACGGATCTTTAATGCACTGTTTAGTGTCTGGCAATAGACGATGACTAATAGGTCTTACTCCTTGCTTAAGCCAACGTTTCACCAGGAATGTTTCTAATTTCCTTACCTGCAACCACAAA

Coding sequence (CDS)

ATGGTTCAAGAATTTAGCTGCTTTCTGTTGGCAACAATGTTCTTGTTTTTATCGGCAATCTTAATAATATCCATTGTTTTTGTGTTTAGAAGCATAAAGGGAAGCAAAAAGGTACCGCCTGGGCAAATGGGTCTGCCCTGGATTGGCGAAACCATGGCTTTCTACAGAGCCCAACGTAAAAACAGATTGTTCGAAGAGTTTGTTGGGCCTCGAGTGGCTAAATATGGCAAAATCTTCAAAACAAGCTTGATGGGGTCGCCGACGGTGGTCGTAAATGGAGCTGAAGCCAACAGATTTTTCTTGTCCAATGAGTTTAAGTTGGTGGTAAGCTCATGGCCGTCGGCCTCTGTTCAGCTGATGGGCGAAGAATGCATCATGCAGAAGCAAGGTGAAAGCCACCGATGTGCGCGTGGCCTTATTGCCGCAAGCCTTGCCAGCTCGAGTCTCGAAGCAATGATGCCTAAATTGTGCACTACAATTCAGCTTTATTTGGACACCAAATGGGTTGGCCACGACATAATTAGTCTTTACCACTCGGCAAAAGTATTGACGTTTACCATAGTGTTTGAGTGTTTGATGGGTATCAAAGTGGAGCCACGCATGTTGACGACATTCGAGAGGGTTTTGGAAGGAGTTTTTGCACCACCGTTCAGTTTTCCAGGCACACGATTTTCGAGAGCGAAGAAAGCAAGGATGGAGATAGGGAAAATGCTAAGGGAGGTGGTGAGAGAGAAGAGGAGGAGAATTGAAGAAGAGCCCGAAGGAGAAGAATGTGAGTTGTTGCTTTCAAGGATGGTGAGAGCAATGATAAGAGGGGAGATCAGTGAAGTGGAAGTGGTGGACAATGTGGTTTTGCTTGTTTTTGCTGCTCATGATACAACCTCCTTCGCCATTGCCGTGACTTTCAAAATGTTGGCTCAGCAGCCAAACTGCTACTCACTTCTTCTACAGGAACATGCTGACATAGTGAGAAGCAAGGGACCAGGCCAGAATTTGACAATGGAAGATATAAAGAAGATGAAATATACTTGGCAGGTAGCTCGTGAGAGCATTCGACTGTTTCCTCCTATATTTGGTTCATTCAGGAAAGCCATTACAGACATTGAATACGAGGGATTTTTCATTCCCAAGGGATGGAAGGTCTTGTGGACGACGTATGGAACGCATCTCAAAGACGAGTATTTTGAAGATCCATTGAGTTTCAAGCCAAGCAGATTTGAGGAACCTGTTTCCCAGTATGTCTATGTTCCATTTGGAGGAGGGCCAAGAGCTTGTGCAGGGTACCAGCTGGCGAAATTGAACATCCTCATCTTTATACACTTGGTTGTAACTCGTTATGATTGGAGTTTAATCCACCCAGATGAGCCAATCATAATGGATCCCCTCCCTATCCCTTCTCATGGAATGCCAATTAAGATTTCTCCAAAATCAGAATCTCATCCCAATGTTCAGCAAATGTAA

Protein sequence

MVQEFSCFLLATMFLFLSAILIISIVFVFRSIKGSKKVPPGQMGLPWIGETMAFYRAQRKNRLFEEFVGPRVAKYGKIFKTSLMGSPTVVVNGAEANRFFLSNEFKLVVSSWPSASVQLMGEECIMQKQGESHRCARGLIAASLASSSLEAMMPKLCTTIQLYLDTKWVGHDIISLYHSAKVLTFTIVFECLMGIKVEPRMLTTFERVLEGVFAPPFSFPGTRFSRAKKARMEIGKMLREVVREKRRRIEEEPEGEECELLLSRMVRAMIRGEISEVEVVDNVVLLVFAAHDTTSFAIAVTFKMLAQQPNCYSLLLQEHADIVRSKGPGQNLTMEDIKKMKYTWQVARESIRLFPPIFGSFRKAITDIEYEGFFIPKGWKVLWTTYGTHLKDEYFEDPLSFKPSRFEEPVSQYVYVPFGGGPRACAGYQLAKLNILIFIHLVVTRYDWSLIHPDEPIIMDPLPIPSHGMPIKISPKSESHPNVQQM

Homology

[Close]

BLAST of Carg15690 vs. NCBI nr
Match: KAG7012036.1 (hypothetical protein SDJN02_26944, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 964.9 bits (2493), Expect = 2.6e-277
Identity = 486/486 (100.00%), Postives = 486/486 (100.00%), Query Frame = 0


Query: 1   MVQEFSCFLLATMFLFLSAILIISIVFVFRSIKGSKKVPPGQMGLPWIGETMAFYRAQRK 60
           MVQEFSCFLLATMFLFLSAILIISIVFVFRSIKGSKKVPPGQMGLPWIGETMAFYRAQRK
Sbjct: 1   MVQEFSCFLLATMFLFLSAILIISIVFVFRSIKGSKKVPPGQMGLPWIGETMAFYRAQRK 60

Query: 61  NRLFEEFVGPRVAKYGKIFKTSLMGSPTVVVNGAEANRFFLSNEFKLVVSSWPSASVQLM 120
           NRLFEEFVGPRVAKYGKIFKTSLMGSPTVVVNGAEANRFFLSNEFKLVVSSWPSASVQLM
Sbjct: 61  NRLFEEFVGPRVAKYGKIFKTSLMGSPTVVVNGAEANRFFLSNEFKLVVSSWPSASVQLM 120

Query: 121 GEECIMQKQGESHRCARGLIAASLASSSLEAMMPKLCTTIQLYLDTKWVGHDIISLYHSA 180
           GEECIMQKQGESHRCARGLIAASLASSSLEAMMPKLCTTIQLYLDTKWVGHDIISLYHSA
Sbjct: 121 GEECIMQKQGESHRCARGLIAASLASSSLEAMMPKLCTTIQLYLDTKWVGHDIISLYHSA 180

Query: 181 KVLTFTIVFECLMGIKVEPRMLTTFERVLEGVFAPPFSFPGTRFSRAKKARMEIGKMLRE 240
           KVLTFTIVFECLMGIKVEPRMLTTFERVLEGVFAPPFSFPGTRFSRAKKARMEIGKMLRE
Sbjct: 181 KVLTFTIVFECLMGIKVEPRMLTTFERVLEGVFAPPFSFPGTRFSRAKKARMEIGKMLRE 240

Query: 241 VVREKRRRIEEEPEGEECELLLSRMVRAMIRGEISEVEVVDNVVLLVFAAHDTTSFAIAV 300
           VVREKRRRIEEEPEGEECELLLSRMVRAMIRGEISEVEVVDNVVLLVFAAHDTTSFAIAV
Sbjct: 241 VVREKRRRIEEEPEGEECELLLSRMVRAMIRGEISEVEVVDNVVLLVFAAHDTTSFAIAV 300

Query: 301 TFKMLAQQPNCYSLLLQEHADIVRSKGPGQNLTMEDIKKMKYTWQVARESIRLFPPIFGS 360
           TFKMLAQQPNCYSLLLQEHADIVRSKGPGQNLTMEDIKKMKYTWQVARESIRLFPPIFGS
Sbjct: 301 TFKMLAQQPNCYSLLLQEHADIVRSKGPGQNLTMEDIKKMKYTWQVARESIRLFPPIFGS 360

Query: 361 FRKAITDIEYEGFFIPKGWKVLWTTYGTHLKDEYFEDPLSFKPSRFEEPVSQYVYVPFGG 420
           FRKAITDIEYEGFFIPKGWKVLWTTYGTHLKDEYFEDPLSFKPSRFEEPVSQYVYVPFGG
Sbjct: 361 FRKAITDIEYEGFFIPKGWKVLWTTYGTHLKDEYFEDPLSFKPSRFEEPVSQYVYVPFGG 420

Query: 421 GPRACAGYQLAKLNILIFIHLVVTRYDWSLIHPDEPIIMDPLPIPSHGMPIKISPKSESH 480
           GPRACAGYQLAKLNILIFIHLVVTRYDWSLIHPDEPIIMDPLPIPSHGMPIKISPKSESH
Sbjct: 421 GPRACAGYQLAKLNILIFIHLVVTRYDWSLIHPDEPIIMDPLPIPSHGMPIKISPKSESH 480

Query: 481 PNVQQM 487
           PNVQQM
Sbjct: 481 PNVQQM 486

[Close]

BLAST of Carg15690 vs. NCBI nr
Match: XP_023554746.1 (taxadiene 5-alpha hydroxylase isoform X2 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 944.5 bits (2440), Expect = 3.6e-271
Identity = 474/486 (97.53%), Postives = 481/486 (98.97%), Query Frame = 0


Query: 1   MVQEFSCFLLATMFLFLSAILIISIVFVFRSIKGSKKVPPGQMGLPWIGETMAFYRAQRK 60
           MVQEFSCFLLATMFLFLSAILIISI+FVFRSIKGSKKVPPGQMGLPWIGETMAFYRAQRK
Sbjct: 1   MVQEFSCFLLATMFLFLSAILIISIIFVFRSIKGSKKVPPGQMGLPWIGETMAFYRAQRK 60

Query: 61  NRLFEEFVGPRVAKYGKIFKTSLMGSPTVVVNGAEANRFFLSNEFKLVVSSWPSASVQLM 120
           NRLFEEFVGPRVAKYGKIFKTSLMGSPTVVVNGAEANRFFLSNEFKLVVSSWPSASVQLM
Sbjct: 61  NRLFEEFVGPRVAKYGKIFKTSLMGSPTVVVNGAEANRFFLSNEFKLVVSSWPSASVQLM 120

Query: 121 GEECIMQKQGESHRCARGLIAASLASSSLEAMMPKLCTTIQLYLDTKWVGHDIISLYHSA 180
           GEECIMQKQGESHRC RGLIAASLASSSLEAMMPKLCTTIQLYLDTKW+GHDIISLYHSA
Sbjct: 121 GEECIMQKQGESHRCVRGLIAASLASSSLEAMMPKLCTTIQLYLDTKWLGHDIISLYHSA 180

Query: 181 KVLTFTIVFECLMGIKVEPRMLTTFERVLEGVFAPPFSFPGTRFSRAKKARMEIGKMLRE 240
           KVLTFTIVFECLMGIKVEPRMLTTFERVLEGVFAPPFSFPGTRFSRAKKARMEIGKMLRE
Sbjct: 181 KVLTFTIVFECLMGIKVEPRMLTTFERVLEGVFAPPFSFPGTRFSRAKKARMEIGKMLRE 240

Query: 241 VVREKRRRIEEEPEGEECELLLSRMVRAMIRGEISEVEVVDNVVLLVFAAHDTTSFAIAV 300
           VVREKRRRIEEEPE EECELLLSRMVRAMIRGEISEVEVVDNVVLLVFAAHDTTSFAIA+
Sbjct: 241 VVREKRRRIEEEPEEEECELLLSRMVRAMIRGEISEVEVVDNVVLLVFAAHDTTSFAIAM 300

Query: 301 TFKMLAQQPNCYSLLLQEHADIVRSKGPGQNLTMEDIKKMKYTWQVARESIRLFPPIFGS 360
           TFKMLAQQPNCYSLLLQEHADIVRSKG GQN+TMEDIKKMKYTWQVARESIRLFPPIFGS
Sbjct: 301 TFKMLAQQPNCYSLLLQEHADIVRSKGAGQNMTMEDIKKMKYTWQVARESIRLFPPIFGS 360

Query: 361 FRKAITDIEYEGFFIPKGWKVLWTTYGTHLKDEYFEDPLSFKPSRFEEPVSQYVYVPFGG 420
           FRKAITDIEYEGFFIPKGWKVLWTTYGTHL+DEYFEDPLSFKPSRFEEPVSQYVYVPFGG
Sbjct: 361 FRKAITDIEYEGFFIPKGWKVLWTTYGTHLEDEYFEDPLSFKPSRFEEPVSQYVYVPFGG 420

Query: 421 GPRACAGYQLAKLNILIFIHLVVTRYDWSLIHPDEPIIMDPLPIPSHGMPIKISPKSESH 480
           GPRACAGYQLAKLNILIFIHLVVTRYDWSL+HPDE IIMDPLPIPSHGMPIKISPKSE+ 
Sbjct: 421 GPRACAGYQLAKLNILIFIHLVVTRYDWSLVHPDETIIMDPLPIPSHGMPIKISPKSEAR 480

Query: 481 PNVQQM 487
           PNVQQM
Sbjct: 481 PNVQQM 486

[Close]

BLAST of Carg15690 vs. NCBI nr
Match: XP_022952282.1 (taxadiene 5-alpha hydroxylase isoform X2 [Cucurbita moschata])

HSP 1 Score: 938.7 bits (2425), Expect = 2.0e-269
Identity = 471/486 (96.91%), Postives = 479/486 (98.56%), Query Frame = 0


Query: 1   MVQEFSCFLLATMFLFLSAILIISIVFVFRSIKGSKKVPPGQMGLPWIGETMAFYRAQRK 60
           MVQEFSCFLLATMFLFLSAILIISI+FVFRSIKGSKKVPPGQMGLPWIGETM FYRAQRK
Sbjct: 1   MVQEFSCFLLATMFLFLSAILIISIIFVFRSIKGSKKVPPGQMGLPWIGETMVFYRAQRK 60

Query: 61  NRLFEEFVGPRVAKYGKIFKTSLMGSPTVVVNGAEANRFFLSNEFKLVVSSWPSASVQLM 120
           NRLFEEFVGPRVAKYGKIFKTSLMGSPTVVVNGAEANRFFLSNEFKLVVSSWPSASVQLM
Sbjct: 61  NRLFEEFVGPRVAKYGKIFKTSLMGSPTVVVNGAEANRFFLSNEFKLVVSSWPSASVQLM 120

Query: 121 GEECIMQKQGESHRCARGLIAASLASSSLEAMMPKLCTTIQLYLDTKWVGHDIISLYHSA 180
           GEECIMQKQGESHRC RGLIAASLASSSLEAMMPKLCTTIQLYLDTKW+GHDIISLYHSA
Sbjct: 121 GEECIMQKQGESHRCVRGLIAASLASSSLEAMMPKLCTTIQLYLDTKWLGHDIISLYHSA 180

Query: 181 KVLTFTIVFECLMGIKVEPRMLTTFERVLEGVFAPPFSFPGTRFSRAKKARMEIGKMLRE 240
           KVLTFTIVFECLMGIKVEPRMLTTFERVLEGVFAPPFSFPGTRFSRAKKARMEIGKMLRE
Sbjct: 181 KVLTFTIVFECLMGIKVEPRMLTTFERVLEGVFAPPFSFPGTRFSRAKKARMEIGKMLRE 240

Query: 241 VVREKRRRIEEEPEGEECELLLSRMVRAMIRGEISEVEVVDNVVLLVFAAHDTTSFAIAV 300
           VVREKRRRIEEEP  EECELLLSRMVRAMIRGEISEVEVVDNVVLLVFAAHDTTSFAIA+
Sbjct: 241 VVREKRRRIEEEPVEEECELLLSRMVRAMIRGEISEVEVVDNVVLLVFAAHDTTSFAIAM 300

Query: 301 TFKMLAQQPNCYSLLLQEHADIVRSKGPGQNLTMEDIKKMKYTWQVARESIRLFPPIFGS 360
           TFKMLAQQPNCYSLLLQEHADIVRSKG GQN+TMEDIKKMKYTWQVARESIRLFPPIFGS
Sbjct: 301 TFKMLAQQPNCYSLLLQEHADIVRSKGAGQNMTMEDIKKMKYTWQVARESIRLFPPIFGS 360

Query: 361 FRKAITDIEYEGFFIPKGWKVLWTTYGTHLKDEYFEDPLSFKPSRFEEPVSQYVYVPFGG 420
           FRKAITDIEYEGFFIPKGWKVLWTTYGTHL+D+YFEDPLSFKPSRFEEPVSQYVYVPFGG
Sbjct: 361 FRKAITDIEYEGFFIPKGWKVLWTTYGTHLEDKYFEDPLSFKPSRFEEPVSQYVYVPFGG 420

Query: 421 GPRACAGYQLAKLNILIFIHLVVTRYDWSLIHPDEPIIMDPLPIPSHGMPIKISPKSESH 480
           GPRACAGYQLAKLNILIFIHLVVTRYDWSL+HPDE IIMDPLPIPSHGMPIKISPKSE+ 
Sbjct: 421 GPRACAGYQLAKLNILIFIHLVVTRYDWSLVHPDETIIMDPLPIPSHGMPIKISPKSEAR 480

Query: 481 PNVQQM 487
           PNVQQM
Sbjct: 481 PNVQQM 486

[Close]

BLAST of Carg15690 vs. NCBI nr
Match: XP_023554747.1 (taxadiene 5-alpha hydroxylase isoform X3 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 899.8 bits (2324), Expect = 1.0e-257
Identity = 446/459 (97.17%), Postives = 454/459 (98.91%), Query Frame = 0


Query: 28  VFRSIKGSKKVPPGQMGLPWIGETMAFYRAQRKNRLFEEFVGPRVAKYGKIFKTSLMGSP 87
           ++RSIKGSKKVPPGQMGLPWIGETMAFYRAQRKNRLFEEFVGPRVAKYGKIFKTSLMGSP
Sbjct: 16  LYRSIKGSKKVPPGQMGLPWIGETMAFYRAQRKNRLFEEFVGPRVAKYGKIFKTSLMGSP 75

Query: 88  TVVVNGAEANRFFLSNEFKLVVSSWPSASVQLMGEECIMQKQGESHRCARGLIAASLASS 147
           TVVVNGAEANRFFLSNEFKLVVSSWPSASVQLMGEECIMQKQGESHRC RGLIAASLASS
Sbjct: 76  TVVVNGAEANRFFLSNEFKLVVSSWPSASVQLMGEECIMQKQGESHRCVRGLIAASLASS 135

Query: 148 SLEAMMPKLCTTIQLYLDTKWVGHDIISLYHSAKVLTFTIVFECLMGIKVEPRMLTTFER 207
           SLEAMMPKLCTTIQLYLDTKW+GHDIISLYHSAKVLTFTIVFECLMGIKVEPRMLTTFER
Sbjct: 136 SLEAMMPKLCTTIQLYLDTKWLGHDIISLYHSAKVLTFTIVFECLMGIKVEPRMLTTFER 195

Query: 208 VLEGVFAPPFSFPGTRFSRAKKARMEIGKMLREVVREKRRRIEEEPEGEECELLLSRMVR 267
           VLEGVFAPPFSFPGTRFSRAKKARMEIGKMLREVVREKRRRIEEEPE EECELLLSRMVR
Sbjct: 196 VLEGVFAPPFSFPGTRFSRAKKARMEIGKMLREVVREKRRRIEEEPEEEECELLLSRMVR 255

Query: 268 AMIRGEISEVEVVDNVVLLVFAAHDTTSFAIAVTFKMLAQQPNCYSLLLQEHADIVRSKG 327
           AMIRGEISEVEVVDNVVLLVFAAHDTTSFAIA+TFKMLAQQPNCYSLLLQEHADIVRSKG
Sbjct: 256 AMIRGEISEVEVVDNVVLLVFAAHDTTSFAIAMTFKMLAQQPNCYSLLLQEHADIVRSKG 315

Query: 328 PGQNLTMEDIKKMKYTWQVARESIRLFPPIFGSFRKAITDIEYEGFFIPKGWKVLWTTYG 387
            GQN+TMEDIKKMKYTWQVARESIRLFPPIFGSFRKAITDIEYEGFFIPKGWKVLWTTYG
Sbjct: 316 AGQNMTMEDIKKMKYTWQVARESIRLFPPIFGSFRKAITDIEYEGFFIPKGWKVLWTTYG 375

Query: 388 THLKDEYFEDPLSFKPSRFEEPVSQYVYVPFGGGPRACAGYQLAKLNILIFIHLVVTRYD 447
           THL+DEYFEDPLSFKPSRFEEPVSQYVYVPFGGGPRACAGYQLAKLNILIFIHLVVTRYD
Sbjct: 376 THLEDEYFEDPLSFKPSRFEEPVSQYVYVPFGGGPRACAGYQLAKLNILIFIHLVVTRYD 435

Query: 448 WSLIHPDEPIIMDPLPIPSHGMPIKISPKSESHPNVQQM 487
           WSL+HPDE IIMDPLPIPSHGMPIKISPKSE+ PNVQQM
Sbjct: 436 WSLVHPDETIIMDPLPIPSHGMPIKISPKSEARPNVQQM 474

[Close]

BLAST of Carg15690 vs. NCBI nr
Match: XP_022969335.1 (taxadiene 5-alpha hydroxylase isoform X1 [Cucurbita maxima])

HSP 1 Score: 898.3 bits (2320), Expect = 3.0e-257
Identity = 452/474 (95.36%), Postives = 462/474 (97.47%), Query Frame = 0


Query: 13  MFLFLSAILIISIVFVFRSIKGSKKVPPGQMGLPWIGETMAFYRAQRKNRLFEEFVGPRV 72
           MFLFLSAILIISI+FVFRSIKGSKKVPPGQMGLPWIG+TMAFYRAQRKNRLFE+FVGPRV
Sbjct: 1   MFLFLSAILIISIIFVFRSIKGSKKVPPGQMGLPWIGQTMAFYRAQRKNRLFEDFVGPRV 60

Query: 73  AKYGKIFKTSLMGSPTVVVNGAEANRFFLSNEFKLVVSSWPSASVQLMGEECIMQKQGES 132
           AKYGKIFKTSLMGSPTVV+NGAEANRFFLSNEFKLVVSSWP+ASVQLMGEECIMQKQGES
Sbjct: 61  AKYGKIFKTSLMGSPTVVINGAEANRFFLSNEFKLVVSSWPAASVQLMGEECIMQKQGES 120

Query: 133 HRCARGLIAASLASSSLEAMMPKLCTTIQLYLDTKWVGHDIISLYHSAKVLTFTIVFECL 192
           HRC RGLIAASLASSSLEAMMPKLCTTIQL+LDTKWVGHDIISLYHSAKVLTF+IVFECL
Sbjct: 121 HRCVRGLIAASLASSSLEAMMPKLCTTIQLHLDTKWVGHDIISLYHSAKVLTFSIVFECL 180

Query: 193 MGIKVEPRMLTTFERVLEGVFAPPFSFPGTRFSRAKKARMEIGKMLREVVREKRRRIEEE 252
           MGIKVEPRMLTTFERVLEGVFA PFSFPGTRFSRAKKARMEIGKMLREVVREKRRRIEEE
Sbjct: 181 MGIKVEPRMLTTFERVLEGVFALPFSFPGTRFSRAKKARMEIGKMLREVVREKRRRIEEE 240

Query: 253 PEGEECELLLSRMVRAMIRGEISEVEVVDNVVLLVFAAHDTTSFAIAVTFKMLAQQPNCY 312
            E EECELLLSRMVRAMIRGEISEVEVVDNVVLLVFAAHDTTSFAIA+TFKMLAQ P+CY
Sbjct: 241 LEEEECELLLSRMVRAMIRGEISEVEVVDNVVLLVFAAHDTTSFAIAMTFKMLAQHPSCY 300

Query: 313 SLLLQEHADIVRSKGPGQNLTMEDIKKMKYTWQVARESIRLFPPIFGSFRKAITDIEYEG 372
           SLLLQEHADIVRSKG GQNLTMEDIKKMKYTWQVARESIRLFPPIFGSFRKAITDIEYEG
Sbjct: 301 SLLLQEHADIVRSKGAGQNLTMEDIKKMKYTWQVARESIRLFPPIFGSFRKAITDIEYEG 360

Query: 373 FFIPKGWKVLWTTYGTHLKDEYFEDPLSFKPSRFEEPVSQYVYVPFGGGPRACAGYQLAK 432
           FFIPKGWKVLWT YGTHL+DEYFEDPLSFKPSRFEEPVSQYVYVPFGGGPRACAGYQLAK
Sbjct: 361 FFIPKGWKVLWTMYGTHLEDEYFEDPLSFKPSRFEEPVSQYVYVPFGGGPRACAGYQLAK 420

Query: 433 LNILIFIHLVVTRYDWSLIHPDEPIIMDPLPIPSHGMPIKISPKSESHPNVQQM 487
           LNILIFIHLVVTRYDWSLIHPDE IIMDPLPIPSHGM  KISPKSE  PNVQQM
Sbjct: 421 LNILIFIHLVVTRYDWSLIHPDETIIMDPLPIPSHGMQTKISPKSEVRPNVQQM 474

[Close]

BLAST of Carg15690 vs. ExPASy Swiss-Prot
Match: Q6WG30 (Taxadiene 5-alpha hydroxylase OS=Taxus cuspidata OX=99806 PE=1 SV=2)

HSP 1 Score: 363.6 bits (932), Expect = 3.4e-99
Identity = 194/471 (41.19%), Postives = 301/471 (63.91%), Query Frame = 0


Query: 17  LSAIL-IISIVFVFRSIK-GSKKVPPGQMGLPWIGETMAFYRAQRKNRLFEEFVGPRVAK 76
           LSAI  I+ ++ +FRS +  S K+PPG++G+P+IGE+  F RA R N L E+F   RV K
Sbjct: 29  LSAIAGILLLLLLFRSKRHSSLKLPPGKLGIPFIGESFIFLRALRSNSL-EQFFDERVKK 88

Query: 77  YGKIFKTSLMGSPTVVVNGAEANRFFLSNEFKLVVSSWPSASVQLMGEECIMQKQGESHR 136
           +G +FKTSL+G PTVV+ G   NR  LSNE KLV  SWP+  ++LMGE  +  ++GE H 
Sbjct: 89  FGLVFKTSLIGHPTVVLCGPAGNRLILSNEEKLVQMSWPAQFMKLMGENSVATRRGEDHI 148

Query: 137 CARGLIAASLASSSLEAMMPKLCTTIQLYLDTKWVGHDIISLYHSAKVLTF---TIVFEC 196
             R  +A      +L++ + K+ T IQ +++ KW G D +++    + L F    I+F  
Sbjct: 149 VMRSALAGFFGPGALQSYIGKMNTEIQSHINEKWKGKDEVNVLPLVRELVFNISAILFFN 208

Query: 197 LMGIKVEPRMLTTFERVLEGVFAPPFSFPGTRFSRAKKARMEIGKMLREVVREKRRRIEE 256
           +   + + R+    E +L G FA P   PG  F RA + R ++ K++  ++++++  ++ 
Sbjct: 209 IYDKQEQDRLHKLLETILVGSFALPIDLPGFGFHRALQGRAKLNKIMLSLIKKRKEDLQS 268

Query: 257 EPEGEECELLLSRMVRAMIRG-EISEVEVVDNVVLLVFAAHDTTSFAIAVTFKMLAQQPN 316
                  +LL   +     +G  ++  E++DN   L+ A++DTT+  +A+ FK+L+  P 
Sbjct: 269 GSATATQDLLSVLLTFRDDKGTPLTNDEILDNFSSLLHASYDTTTSPMALIFKLLSSNPE 328

Query: 317 CYSLLLQEHADIVRSKGPGQNLTMEDIKKMKYTWQVARESIRLFPPIFGSFRKAITDIEY 376
           CY  ++QE  +I+ +K  G+ +T +D+K MKYTWQVA+E++R+FPP+FG+FRKAITDI+Y
Sbjct: 329 CYQKVVQEQLEILSNKEEGEEITWKDLKAMKYTWQVAQETLRMFPPVFGTFRKAITDIQY 388

Query: 377 EGFFIPKGWKVLWTTYGTHLKDEYFEDPLSFKPSRFEEP---VSQYVYVPFGGGPRACAG 436
           +G+ IPKGWK+LWTTY TH KD YF +P  F PSRF++    V+ Y ++PFGGG R+C G
Sbjct: 389 DGYTIPKGWKLLWTTYSTHPKDLYFNEPEKFMPSRFDQEGKHVAPYTFLPFGGGQRSCVG 448

Query: 437 YQLAKLNILIFI-HLVVTRYDWSLIHPDEPIIMDPL-PIPSHGMPIKISPK 477
           ++ +K+ IL+F+ H V T   ++ + PDE I  DPL P+PS G  IK+ P+
Sbjct: 449 WEFSKMEILLFVHHFVKTFSSYTPVDPDEKISGDPLPPLPSKGFSIKLFPR 498

[Close]

BLAST of Carg15690 vs. ExPASy Swiss-Prot
Match: Q8W4T9 (Taxane 13-alpha-hydroxylase OS=Taxus cuspidata OX=99806 GN=CYP725A2 PE=1 SV=1)

HSP 1 Score: 342.0 bits (876), Expect = 1.1e-92
Identity = 180/473 (38.05%), Postives = 288/473 (60.89%), Query Frame = 0


Query: 15  LFLSAILIISIVFVFRSIK-GSKKVPPGQMGLPWIGETMAFYRAQRKNRLFEEFVGPRVA 74
           LF++  ++  I+  FRS +  S K+PPG +G P +GET+ F R+   +   ++F+  R++
Sbjct: 14  LFVTLAVMAGIILFFRSKRHSSVKLPPGNLGFPLVGETLQFVRSLGSSTP-QQFIEERMS 73

Query: 75  KYGKIFKTSLMGSPTVVVNGAEANRFFLSNEFKLVVSSWPSASVQLMGEECIMQKQGESH 134
           K+G +FKTS++G PTVV+ G   NR  LSNE KLV  SWPS+ ++L+GE+C+  K GE H
Sbjct: 74  KFGDVFKTSIIGHPTVVLCGPAGNRLVLSNENKLVQMSWPSSMMKLIGEDCLGGKTGEQH 133

Query: 135 RCARGLIAASLASSSLEAMMPKLCTTIQLYLDTKWVGHDIISLYHSAKVLTFTIVFECLM 194
           R  R  +   L   +L+    K+ + IQ +++ KW G D  ++    K L F++      
Sbjct: 134 RIVRAALTRFLGPQALQNHFAKMSSGIQRHINEKWKGKDEATVLPLVKDLVFSVASRLFF 193

Query: 195 GI---KVEPRMLTTFERVLEGVFAPPFSFPGTRFSRAKKARMEIGKMLREVVREKRRRIE 254
           GI    ++ ++    E +L G F+ P + PG  + +A +AR  +  ++  ++ ++R  + 
Sbjct: 194 GITEEHLQEQLHNLLEVILVGSFSVPLNIPGFSYHKAIQARATLADIMTHLIEKRRNELR 253

Query: 255 EEPEGEECELLLSRMVRAMIRG-EISEVEVVDNVVLLVFAAHDTTSFAIAVTFKMLAQQP 314
                E  +LL   +     RG  +++ E++DN  +L+  ++D+T+  + +  K+LA  P
Sbjct: 254 AGTASENQDLLSVLLTFTDERGNSLADKEILDNFSMLLHGSYDSTNSPLTMLIKVLASHP 313

Query: 315 NCYSLLLQEHADIVRSKGPGQNLTMEDIKKMKYTWQVARESIRLFPPIFGSFRKAITDIE 374
             Y  + QE   I+ +K  G+ +  +D+K+MKY+WQV +E++R++PPIFG+FRKAITDI 
Sbjct: 314 ESYEKVAQEQFGILSTKMEGEEIAWKDLKEMKYSWQVVQETLRMYPPIFGTFRKAITDIH 373

Query: 375 YEGFFIPKGWKVLWTTYGTHLKDEYFEDPLSFKPSRFEEP---VSQYVYVPFGGGPRACA 434
           Y G+ IPKGWK+LWTTY T  K+EYF+D   FKPSRFEE    V+ Y Y+PFGGG R C 
Sbjct: 374 YNGYTIPKGWKLLWTTYSTQTKEEYFKDADQFKPSRFEEEGKHVTPYTYLPFGGGMRVCP 433

Query: 435 GYQLAKLNILIFIHLVVTRYD-WSLIHPDEPIIMDPL-PIPSHGMPIKISPKS 478
           G++ AK+  L+F+H  V  +     I P+E +   PL P+P +G+PIK+  +S
Sbjct: 434 GWEFAKMETLLFLHHFVKAFSGLKAIDPNEKLSGKPLPPLPVNGLPIKLYSRS 485

[Close]

BLAST of Carg15690 vs. ExPASy Swiss-Prot
Match: Q50EK0 (Cytochrome P450 716B2 OS=Picea sitchensis OX=3332 GN=CYP716B2 PE=2 SV=1)

HSP 1 Score: 335.1 bits (858), Expect = 1.3e-90
Identity = 185/469 (39.45%), Postives = 276/469 (58.85%), Query Frame = 0


Query: 15  LFLSAILIISIVFVFRS---IKGSKKVPPGQMGLPWIGETMAFYRAQRKNRLFEEFVGPR 74
           +FL++ + ++  F   S    K  + +PPG  G P IGET+ F   QRK     +F   R
Sbjct: 25  VFLASFIGLAFFFYLISNHRTKAWRGIPPGTFGWPLIGETLEFLGCQRKGNP-RDFFDSR 84

Query: 75  VAKYGKIFKTSLMGSPTVVVNGAEANRFFLSNEFKLVVSSWPSASVQLMGEECIMQKQGE 134
             KYG +F TSL+G PTVV    E NRF  SNE KLVV+SWPS SV  +    ++   G+
Sbjct: 85  TQKYGNVFTTSLVGHPTVVFCSPEGNRFLFSNENKLVVNSWPS-SVGNLFRSSLITTVGD 144

Query: 135 SHRCARGLIAASLASSSLEAMMPKLCTTIQLYLDTKWVGHDIISLYHSAKVLTFTIVFEC 194
             +  R ++   L   +L   + ++ +  + +L   W+G D ++     K  TF++  + 
Sbjct: 145 DAKRLRRILMTFLRPEALREFVGRVDSMTKRHLAEHWIGKDEVTALPLLKRYTFSLACDL 204

Query: 195 LMGIKVEP---RMLTTFERVLEGVFAPPFSFPGTRFSRAKKARMEIGKMLREVVREKRRR 254
              I  +    R+   F   ++GV   P   PGTR+++AK A   I + L  ++ E++  
Sbjct: 205 FASINNKDDLGRLWLHFMVFVKGVMQIPIDLPGTRYNKAKHAANAIRQQLGSIINERKIG 264

Query: 255 IEEEPEGEECELLLSRMVRAMIRGE-ISEVEVVDNVVLLVFAAHDTTSFAIAVTFKMLAQ 314
           +E      E +LL   +     +GE +++ E+ DN++LL++A HDT+S  + V  K LA+
Sbjct: 265 LEAGNASPEQDLLSFLLSNVDEQGESLTDNEIQDNILLLLYAGHDTSSSTLTVLLKFLAE 324

Query: 315 QPNCYSLLLQEHADIVRSKGPGQNLTMEDIKKMKYTWQVARESIRLFPPIFGSFRKAITD 374
            P+CY  +L+E  DI  SK  GQ L  ED+++MKY+W+VA+E++RLFP   GSFRKAI +
Sbjct: 325 NPHCYEEVLREQLDIAGSKEAGQLLEWEDLQRMKYSWRVAQEALRLFPAAQGSFRKAIKE 384

Query: 375 IEYEGFFIPKGWKVLWTTYGTHLKDEYFEDPLSFKPSRFE-EPVSQYVYVPFGGGPRACA 434
             Y+GF IPKGWK+ WT   TH K EYF +P +F PSRFE E    Y +VPFGGGPR C 
Sbjct: 385 FIYDGFTIPKGWKMYWTVNSTHRKSEYFSNPETFDPSRFEGEGPPPYTFVPFGGGPRMCP 444

Query: 435 GYQLAKLNILIFIHLVVTRYDWSLIHPDEPIIMDPLPIPSHGMPIKISP 476
           G + A+L IL+F+H +V    W+L++P E +I+DP+P P +G+PIK+ P
Sbjct: 445 GNEFARLEILVFLHNIVKNCKWNLVNPGEKVIVDPMPAPVNGLPIKLVP 491

[Close]

BLAST of Carg15690 vs. ExPASy Swiss-Prot
Match: Q50EK1 (Cytochrome P450 716B1 OS=Picea sitchensis OX=3332 GN=CYP716B1 PE=2 SV=1)

HSP 1 Score: 334.0 bits (855), Expect = 2.9e-90
Identity = 183/469 (39.02%), Postives = 276/469 (58.85%), Query Frame = 0


Query: 15  LFLSAILIISIVFVFRS---IKGSKKVPPGQMGLPWIGETMAFYRAQRKNRLFEEFVGPR 74
           +FL++ + ++  F   S    K  + +PPG  G P IGET+ F   QRK     +F   R
Sbjct: 25  VFLASFIGLAFFFYLISNHRTKAWRGIPPGTFGWPLIGETLEFLGCQRKGNP-RDFFDSR 84

Query: 75  VAKYGKIFKTSLMGSPTVVVNGAEANRFFLSNEFKLVVSSWPSASVQLMGEECIMQKQGE 134
             KYG +F TSL+G PTVV    E NRF  SNE KLVV+SWPS SV  +    ++   G+
Sbjct: 85  TQKYGNVFTTSLVGHPTVVFCSPEGNRFLFSNENKLVVNSWPS-SVGNLFRSSLITTVGD 144

Query: 135 SHRCARGLIAASLASSSLEAMMPKLCTTIQLYLDTKWVGHDIISLYHSAKVLTFTIVFEC 194
             +  R ++   L   +L   + ++ +  + +L   W+G D +      K  TF++  + 
Sbjct: 145 DAKRLRRILMTFLRPEALREFVGRVDSMTKRHLAEHWIGKDEVMALPLLKRYTFSLACDL 204

Query: 195 LMGIKVE---PRMLTTFERVLEGVFAPPFSFPGTRFSRAKKARMEIGKMLREVVREKRRR 254
              I  +    R+   F   ++GV   P   PGTR+++ K A   I + L  ++ E++  
Sbjct: 205 FASINTKDDLDRLWLHFMVFVKGVMQIPIDLPGTRYNKTKHAANAIRQQLGSIINERKIA 264

Query: 255 IEEEPEGEECELLLSRMVRAMIRGE-ISEVEVVDNVVLLVFAAHDTTSFAIAVTFKMLAQ 314
           +E      E +LL   +     +GE +++ E+ DN++LL++A HDT+S  + V  K LA+
Sbjct: 265 LEAGNASPEQDLLSFLLSNVDEQGESLTDNEIQDNILLLLYAGHDTSSSTLTVLLKFLAE 324

Query: 315 QPNCYSLLLQEHADIVRSKGPGQNLTMEDIKKMKYTWQVARESIRLFPPIFGSFRKAITD 374
            P+CY  +L+E  +I  SK  GQ L  ED+++MKY+W+VA+E++RLFP + GSFRKAI +
Sbjct: 325 NPHCYEEVLREQLNIAGSKEEGQLLEWEDLQRMKYSWRVAQEALRLFPAVQGSFRKAIKE 384

Query: 375 IEYEGFFIPKGWKVLWTTYGTHLKDEYFEDPLSFKPSRFE-EPVSQYVYVPFGGGPRACA 434
             Y+GF IPKGWK+ WT   TH K EYF +P  F PSRFE E    Y +VPFGGGPR C 
Sbjct: 385 FIYDGFTIPKGWKLHWTVNSTHQKSEYFSNPEKFDPSRFEGEGPPPYTFVPFGGGPRMCP 444

Query: 435 GYQLAKLNILIFIHLVVTRYDWSLIHPDEPIIMDPLPIPSHGMPIKISP 476
           G + A++ ILIF+H +V  ++W+L++P E +I+DP+P P +G+PIK+ P
Sbjct: 445 GNEFARMEILIFLHNIVKNFNWNLVNPLEKVIVDPMPAPVNGLPIKLVP 491

[Close]

BLAST of Carg15690 vs. ExPASy Swiss-Prot
Match: Q84KI1 (Taxoid 14-beta-hydroxylase OS=Taxus cuspidata OX=99806 PE=1 SV=1)

HSP 1 Score: 330.5 bits (846), Expect = 3.2e-89
Identity = 187/490 (38.16%), Postives = 293/490 (59.80%), Query Frame = 0


Query: 2   VQEFSCFLLATMFLFLSAI--LIISIVFVFRS-IKGSKKVPPGQMGLPWIGETMAFYRAQ 61
           V +F+    A +F+ LSA+  +++ ++   RS  + S  +PPG++G P+IGE++ F +A 
Sbjct: 11  VAKFNECFPAILFIVLSAVAGIVLPLLLFLRSKRRSSVGLPPGKLGYPFIGESLLFLKAL 70

Query: 62  RKNRLFEEFVGPRVAKYGKIFKTSLMGSPTVVVNGAEANRFFLSNEFKLVVSSWPSASVQ 121
           R N + E+F+  RV  +G +FKTSL+G PTVV+ G   NR  L+NE KLV  SWP +S++
Sbjct: 71  RSNTV-EQFLDERVKNFGNVFKTSLIGHPTVVLCGPAGNRLILANEEKLVQMSWPKSSMK 130

Query: 122 LMGEECIMQKQGESHRCARGLIAASLASSSLEAMMPKLCTTIQLYLDTKWVGHDIISLYH 181
           LMGE+ I  K+GE H   R  +    +  +L+  + ++  TI+ +++ KW G+D +S+  
Sbjct: 131 LMGEKSITAKRGEGHMIIRSALQGFFSPGALQKYIGQMSKTIENHINEKWKGNDQVSVVA 190

Query: 182 SAKVLTFTIVFECLMGIKVE---PRMLTTFERVLEGVFAPPFSFPGTRFSRAKKARMEIG 241
               L F I       I  +    R+    E +  GV A P   PG  + RA +AR ++ 
Sbjct: 191 LVGDLVFDISACLFFNINEKHERERLFELLEIIAVGVLAVPVDLPGFAYHRALQARSKLN 250

Query: 242 KMLREVVREKRRRIEEEPEGEECELLLSRMVRAMIRGE-ISEVEVVDNVVLLVFAAHDTT 301
            +L  ++ +++  +         +LL   +     RG   S+ E++DN   L+  ++DTT
Sbjct: 251 AILSGLIEKRKMDLSSGLATSNQDLLSVFLTFKDDRGNPCSDEEILDNFSGLLHGSYDTT 310

Query: 302 SFAIAVTFKMLAQQPNCYSLLLQEHADIVRSKGPGQNLTMEDIKKMKYTWQVARESIRLF 361
             A+A  FK+L+  P CY  ++QE   I+ +K  G  +T +D+K MKYTWQV +E++RL+
Sbjct: 311 VSAMACVFKLLSSNPECYEKVVQEQLGILSNKLEGDEITWKDVKSMKYTWQVVQETLRLY 370

Query: 362 PPIFGSFRKAITDIEYEGFFIPKGWKVLWTTYGTHLKDEYFEDPLSFKPSRFEEP---VS 421
           P IFGSFR+AITDI Y G+ IPKGWK+LWT Y TH K+ YF +P  F PSRF++    V+
Sbjct: 371 PSIFGSFRQAITDIHYNGYIIPKGWKLLWTPYTTHPKEMYFSEPEKFLPSRFDQEGKLVA 430

Query: 422 QYVYVPFGGGPRACAGYQLAKLNILIFI-HLVVTRYDWSLIHPDEPIIMDPL-PIPSHGM 480
            Y ++PFGGG R+C G++ +K+ IL+ + H V T   ++ + P E I  D L P+PS+G 
Sbjct: 431 PYTFLPFGGGQRSCPGWEFSKMEILLSVHHFVKTFSTFTPVDPAEIIARDSLCPLPSNGF 490

[Close]

BLAST of Carg15690 vs. ExPASy TrEMBL
Match: A0A6J1GJY9 (taxadiene 5-alpha hydroxylase isoform X2 OS=Cucurbita moschata OX=3662 GN=LOC111455006 PE=3 SV=1)

HSP 1 Score: 938.7 bits (2425), Expect = 9.6e-270
Identity = 471/486 (96.91%), Postives = 479/486 (98.56%), Query Frame = 0


Query: 1   MVQEFSCFLLATMFLFLSAILIISIVFVFRSIKGSKKVPPGQMGLPWIGETMAFYRAQRK 60
           MVQEFSCFLLATMFLFLSAILIISI+FVFRSIKGSKKVPPGQMGLPWIGETM FYRAQRK
Sbjct: 1   MVQEFSCFLLATMFLFLSAILIISIIFVFRSIKGSKKVPPGQMGLPWIGETMVFYRAQRK 60

Query: 61  NRLFEEFVGPRVAKYGKIFKTSLMGSPTVVVNGAEANRFFLSNEFKLVVSSWPSASVQLM 120
           NRLFEEFVGPRVAKYGKIFKTSLMGSPTVVVNGAEANRFFLSNEFKLVVSSWPSASVQLM
Sbjct: 61  NRLFEEFVGPRVAKYGKIFKTSLMGSPTVVVNGAEANRFFLSNEFKLVVSSWPSASVQLM 120

Query: 121 GEECIMQKQGESHRCARGLIAASLASSSLEAMMPKLCTTIQLYLDTKWVGHDIISLYHSA 180
           GEECIMQKQGESHRC RGLIAASLASSSLEAMMPKLCTTIQLYLDTKW+GHDIISLYHSA
Sbjct: 121 GEECIMQKQGESHRCVRGLIAASLASSSLEAMMPKLCTTIQLYLDTKWLGHDIISLYHSA 180

Query: 181 KVLTFTIVFECLMGIKVEPRMLTTFERVLEGVFAPPFSFPGTRFSRAKKARMEIGKMLRE 240
           KVLTFTIVFECLMGIKVEPRMLTTFERVLEGVFAPPFSFPGTRFSRAKKARMEIGKMLRE
Sbjct: 181 KVLTFTIVFECLMGIKVEPRMLTTFERVLEGVFAPPFSFPGTRFSRAKKARMEIGKMLRE 240

Query: 241 VVREKRRRIEEEPEGEECELLLSRMVRAMIRGEISEVEVVDNVVLLVFAAHDTTSFAIAV 300
           VVREKRRRIEEEP  EECELLLSRMVRAMIRGEISEVEVVDNVVLLVFAAHDTTSFAIA+
Sbjct: 241 VVREKRRRIEEEPVEEECELLLSRMVRAMIRGEISEVEVVDNVVLLVFAAHDTTSFAIAM 300

Query: 301 TFKMLAQQPNCYSLLLQEHADIVRSKGPGQNLTMEDIKKMKYTWQVARESIRLFPPIFGS 360
           TFKMLAQQPNCYSLLLQEHADIVRSKG GQN+TMEDIKKMKYTWQVARESIRLFPPIFGS
Sbjct: 301 TFKMLAQQPNCYSLLLQEHADIVRSKGAGQNMTMEDIKKMKYTWQVARESIRLFPPIFGS 360

Query: 361 FRKAITDIEYEGFFIPKGWKVLWTTYGTHLKDEYFEDPLSFKPSRFEEPVSQYVYVPFGG 420
           FRKAITDIEYEGFFIPKGWKVLWTTYGTHL+D+YFEDPLSFKPSRFEEPVSQYVYVPFGG
Sbjct: 361 FRKAITDIEYEGFFIPKGWKVLWTTYGTHLEDKYFEDPLSFKPSRFEEPVSQYVYVPFGG 420

Query: 421 GPRACAGYQLAKLNILIFIHLVVTRYDWSLIHPDEPIIMDPLPIPSHGMPIKISPKSESH 480
           GPRACAGYQLAKLNILIFIHLVVTRYDWSL+HPDE IIMDPLPIPSHGMPIKISPKSE+ 
Sbjct: 421 GPRACAGYQLAKLNILIFIHLVVTRYDWSLVHPDETIIMDPLPIPSHGMPIKISPKSEAR 480

Query: 481 PNVQQM 487
           PNVQQM
Sbjct: 481 PNVQQM 486

[Close]

BLAST of Carg15690 vs. ExPASy TrEMBL
Match: A0A6J1HZM9 (taxadiene 5-alpha hydroxylase isoform X1 OS=Cucurbita maxima OX=3661 GN=LOC111468378 PE=3 SV=1)

HSP 1 Score: 898.3 bits (2320), Expect = 1.4e-257
Identity = 452/474 (95.36%), Postives = 462/474 (97.47%), Query Frame = 0


Query: 13  MFLFLSAILIISIVFVFRSIKGSKKVPPGQMGLPWIGETMAFYRAQRKNRLFEEFVGPRV 72
           MFLFLSAILIISI+FVFRSIKGSKKVPPGQMGLPWIG+TMAFYRAQRKNRLFE+FVGPRV
Sbjct: 1   MFLFLSAILIISIIFVFRSIKGSKKVPPGQMGLPWIGQTMAFYRAQRKNRLFEDFVGPRV 60

Query: 73  AKYGKIFKTSLMGSPTVVVNGAEANRFFLSNEFKLVVSSWPSASVQLMGEECIMQKQGES 132
           AKYGKIFKTSLMGSPTVV+NGAEANRFFLSNEFKLVVSSWP+ASVQLMGEECIMQKQGES
Sbjct: 61  AKYGKIFKTSLMGSPTVVINGAEANRFFLSNEFKLVVSSWPAASVQLMGEECIMQKQGES 120

Query: 133 HRCARGLIAASLASSSLEAMMPKLCTTIQLYLDTKWVGHDIISLYHSAKVLTFTIVFECL 192
           HRC RGLIAASLASSSLEAMMPKLCTTIQL+LDTKWVGHDIISLYHSAKVLTF+IVFECL
Sbjct: 121 HRCVRGLIAASLASSSLEAMMPKLCTTIQLHLDTKWVGHDIISLYHSAKVLTFSIVFECL 180

Query: 193 MGIKVEPRMLTTFERVLEGVFAPPFSFPGTRFSRAKKARMEIGKMLREVVREKRRRIEEE 252
           MGIKVEPRMLTTFERVLEGVFA PFSFPGTRFSRAKKARMEIGKMLREVVREKRRRIEEE
Sbjct: 181 MGIKVEPRMLTTFERVLEGVFALPFSFPGTRFSRAKKARMEIGKMLREVVREKRRRIEEE 240

Query: 253 PEGEECELLLSRMVRAMIRGEISEVEVVDNVVLLVFAAHDTTSFAIAVTFKMLAQQPNCY 312
            E EECELLLSRMVRAMIRGEISEVEVVDNVVLLVFAAHDTTSFAIA+TFKMLAQ P+CY
Sbjct: 241 LEEEECELLLSRMVRAMIRGEISEVEVVDNVVLLVFAAHDTTSFAIAMTFKMLAQHPSCY 300

Query: 313 SLLLQEHADIVRSKGPGQNLTMEDIKKMKYTWQVARESIRLFPPIFGSFRKAITDIEYEG 372
           SLLLQEHADIVRSKG GQNLTMEDIKKMKYTWQVARESIRLFPPIFGSFRKAITDIEYEG
Sbjct: 301 SLLLQEHADIVRSKGAGQNLTMEDIKKMKYTWQVARESIRLFPPIFGSFRKAITDIEYEG 360

Query: 373 FFIPKGWKVLWTTYGTHLKDEYFEDPLSFKPSRFEEPVSQYVYVPFGGGPRACAGYQLAK 432
           FFIPKGWKVLWT YGTHL+DEYFEDPLSFKPSRFEEPVSQYVYVPFGGGPRACAGYQLAK
Sbjct: 361 FFIPKGWKVLWTMYGTHLEDEYFEDPLSFKPSRFEEPVSQYVYVPFGGGPRACAGYQLAK 420

Query: 433 LNILIFIHLVVTRYDWSLIHPDEPIIMDPLPIPSHGMPIKISPKSESHPNVQQM 487
           LNILIFIHLVVTRYDWSLIHPDE IIMDPLPIPSHGM  KISPKSE  PNVQQM
Sbjct: 421 LNILIFIHLVVTRYDWSLIHPDETIIMDPLPIPSHGMQTKISPKSEVRPNVQQM 474

[Close]

BLAST of Carg15690 vs. ExPASy TrEMBL
Match: A0A6J1GK43 (taxadiene 5-alpha hydroxylase isoform X3 OS=Cucurbita moschata OX=3662 GN=LOC111455006 PE=3 SV=1)

HSP 1 Score: 894.0 bits (2309), Expect = 2.7e-256
Identity = 443/459 (96.51%), Postives = 452/459 (98.47%), Query Frame = 0


Query: 28  VFRSIKGSKKVPPGQMGLPWIGETMAFYRAQRKNRLFEEFVGPRVAKYGKIFKTSLMGSP 87
           ++RSIKGSKKVPPGQMGLPWIGETM FYRAQRKNRLFEEFVGPRVAKYGKIFKTSLMGSP
Sbjct: 16  LYRSIKGSKKVPPGQMGLPWIGETMVFYRAQRKNRLFEEFVGPRVAKYGKIFKTSLMGSP 75

Query: 88  TVVVNGAEANRFFLSNEFKLVVSSWPSASVQLMGEECIMQKQGESHRCARGLIAASLASS 147
           TVVVNGAEANRFFLSNEFKLVVSSWPSASVQLMGEECIMQKQGESHRC RGLIAASLASS
Sbjct: 76  TVVVNGAEANRFFLSNEFKLVVSSWPSASVQLMGEECIMQKQGESHRCVRGLIAASLASS 135

Query: 148 SLEAMMPKLCTTIQLYLDTKWVGHDIISLYHSAKVLTFTIVFECLMGIKVEPRMLTTFER 207
           SLEAMMPKLCTTIQLYLDTKW+GHDIISLYHSAKVLTFTIVFECLMGIKVEPRMLTTFER
Sbjct: 136 SLEAMMPKLCTTIQLYLDTKWLGHDIISLYHSAKVLTFTIVFECLMGIKVEPRMLTTFER 195

Query: 208 VLEGVFAPPFSFPGTRFSRAKKARMEIGKMLREVVREKRRRIEEEPEGEECELLLSRMVR 267
           VLEGVFAPPFSFPGTRFSRAKKARMEIGKMLREVVREKRRRIEEEP  EECELLLSRMVR
Sbjct: 196 VLEGVFAPPFSFPGTRFSRAKKARMEIGKMLREVVREKRRRIEEEPVEEECELLLSRMVR 255

Query: 268 AMIRGEISEVEVVDNVVLLVFAAHDTTSFAIAVTFKMLAQQPNCYSLLLQEHADIVRSKG 327
           AMIRGEISEVEVVDNVVLLVFAAHDTTSFAIA+TFKMLAQQPNCYSLLLQEHADIVRSKG
Sbjct: 256 AMIRGEISEVEVVDNVVLLVFAAHDTTSFAIAMTFKMLAQQPNCYSLLLQEHADIVRSKG 315

Query: 328 PGQNLTMEDIKKMKYTWQVARESIRLFPPIFGSFRKAITDIEYEGFFIPKGWKVLWTTYG 387
            GQN+TMEDIKKMKYTWQVARESIRLFPPIFGSFRKAITDIEYEGFFIPKGWKVLWTTYG
Sbjct: 316 AGQNMTMEDIKKMKYTWQVARESIRLFPPIFGSFRKAITDIEYEGFFIPKGWKVLWTTYG 375

Query: 388 THLKDEYFEDPLSFKPSRFEEPVSQYVYVPFGGGPRACAGYQLAKLNILIFIHLVVTRYD 447
           THL+D+YFEDPLSFKPSRFEEPVSQYVYVPFGGGPRACAGYQLAKLNILIFIHLVVTRYD
Sbjct: 376 THLEDKYFEDPLSFKPSRFEEPVSQYVYVPFGGGPRACAGYQLAKLNILIFIHLVVTRYD 435

Query: 448 WSLIHPDEPIIMDPLPIPSHGMPIKISPKSESHPNVQQM 487
           WSL+HPDE IIMDPLPIPSHGMPIKISPKSE+ PNVQQM
Sbjct: 436 WSLVHPDETIIMDPLPIPSHGMPIKISPKSEARPNVQQM 474

[Close]

BLAST of Carg15690 vs. ExPASy TrEMBL
Match: A0A6J1GJT6 (taxadiene 5-alpha hydroxylase isoform X1 OS=Cucurbita moschata OX=3662 GN=LOC111455006 PE=3 SV=1)

HSP 1 Score: 891.3 bits (2302), Expect = 1.8e-255
Identity = 443/463 (95.68%), Postives = 452/463 (97.62%), Query Frame = 0


Query: 24  SIVFVFRSIKGSKKVPPGQMGLPWIGETMAFYRAQRKNRLFEEFVGPRVAKYGKIFKTSL 83
           S++    SIKGSKKVPPGQMGLPWIGETM FYRAQRKNRLFEEFVGPRVAKYGKIFKTSL
Sbjct: 37  SVIASLGSIKGSKKVPPGQMGLPWIGETMVFYRAQRKNRLFEEFVGPRVAKYGKIFKTSL 96

Query: 84  MGSPTVVVNGAEANRFFLSNEFKLVVSSWPSASVQLMGEECIMQKQGESHRCARGLIAAS 143
           MGSPTVVVNGAEANRFFLSNEFKLVVSSWPSASVQLMGEECIMQKQGESHRC RGLIAAS
Sbjct: 97  MGSPTVVVNGAEANRFFLSNEFKLVVSSWPSASVQLMGEECIMQKQGESHRCVRGLIAAS 156

Query: 144 LASSSLEAMMPKLCTTIQLYLDTKWVGHDIISLYHSAKVLTFTIVFECLMGIKVEPRMLT 203
           LASSSLEAMMPKLCTTIQLYLDTKW+GHDIISLYHSAKVLTFTIVFECLMGIKVEPRMLT
Sbjct: 157 LASSSLEAMMPKLCTTIQLYLDTKWLGHDIISLYHSAKVLTFTIVFECLMGIKVEPRMLT 216

Query: 204 TFERVLEGVFAPPFSFPGTRFSRAKKARMEIGKMLREVVREKRRRIEEEPEGEECELLLS 263
           TFERVLEGVFAPPFSFPGTRFSRAKKARMEIGKMLREVVREKRRRIEEEP  EECELLLS
Sbjct: 217 TFERVLEGVFAPPFSFPGTRFSRAKKARMEIGKMLREVVREKRRRIEEEPVEEECELLLS 276

Query: 264 RMVRAMIRGEISEVEVVDNVVLLVFAAHDTTSFAIAVTFKMLAQQPNCYSLLLQEHADIV 323
           RMVRAMIRGEISEVEVVDNVVLLVFAAHDTTSFAIA+TFKMLAQQPNCYSLLLQEHADIV
Sbjct: 277 RMVRAMIRGEISEVEVVDNVVLLVFAAHDTTSFAIAMTFKMLAQQPNCYSLLLQEHADIV 336

Query: 324 RSKGPGQNLTMEDIKKMKYTWQVARESIRLFPPIFGSFRKAITDIEYEGFFIPKGWKVLW 383
           RSKG GQN+TMEDIKKMKYTWQVARESIRLFPPIFGSFRKAITDIEYEGFFIPKGWKVLW
Sbjct: 337 RSKGAGQNMTMEDIKKMKYTWQVARESIRLFPPIFGSFRKAITDIEYEGFFIPKGWKVLW 396

Query: 384 TTYGTHLKDEYFEDPLSFKPSRFEEPVSQYVYVPFGGGPRACAGYQLAKLNILIFIHLVV 443
           TTYGTHL+D+YFEDPLSFKPSRFEEPVSQYVYVPFGGGPRACAGYQLAKLNILIFIHLVV
Sbjct: 397 TTYGTHLEDKYFEDPLSFKPSRFEEPVSQYVYVPFGGGPRACAGYQLAKLNILIFIHLVV 456

Query: 444 TRYDWSLIHPDEPIIMDPLPIPSHGMPIKISPKSESHPNVQQM 487
           TRYDWSL+HPDE IIMDPLPIPSHGMPIKISPKSE+ PNVQQM
Sbjct: 457 TRYDWSLVHPDETIIMDPLPIPSHGMPIKISPKSEARPNVQQM 499

[Close]

BLAST of Carg15690 vs. ExPASy TrEMBL
Match: A0A6J1HXH8 (taxadiene 5-alpha hydroxylase isoform X2 OS=Cucurbita maxima OX=3661 GN=LOC111468378 PE=3 SV=1)

HSP 1 Score: 874.8 bits (2259), Expect = 1.7e-250
Identity = 436/459 (94.99%), Postives = 447/459 (97.39%), Query Frame = 0


Query: 28  VFRSIKGSKKVPPGQMGLPWIGETMAFYRAQRKNRLFEEFVGPRVAKYGKIFKTSLMGSP 87
           ++RSIKGSKKVPPGQMGLPWIG+TMAFYRAQRKNRLFE+FVGPRVAKYGKIFKTSLMGSP
Sbjct: 16  LYRSIKGSKKVPPGQMGLPWIGQTMAFYRAQRKNRLFEDFVGPRVAKYGKIFKTSLMGSP 75

Query: 88  TVVVNGAEANRFFLSNEFKLVVSSWPSASVQLMGEECIMQKQGESHRCARGLIAASLASS 147
           TVV+NGAEANRFFLSNEFKLVVSSWP+ASVQLMGEECIMQKQGESHRC RGLIAASLASS
Sbjct: 76  TVVINGAEANRFFLSNEFKLVVSSWPAASVQLMGEECIMQKQGESHRCVRGLIAASLASS 135

Query: 148 SLEAMMPKLCTTIQLYLDTKWVGHDIISLYHSAKVLTFTIVFECLMGIKVEPRMLTTFER 207
           SLEAMMPKLCTTIQL+LDTKWVGHDIISLYHSAKVLTF+IVFECLMGIKVEPRMLTTFER
Sbjct: 136 SLEAMMPKLCTTIQLHLDTKWVGHDIISLYHSAKVLTFSIVFECLMGIKVEPRMLTTFER 195

Query: 208 VLEGVFAPPFSFPGTRFSRAKKARMEIGKMLREVVREKRRRIEEEPEGEECELLLSRMVR 267
           VLEGVFA PFSFPGTRFSRAKKARMEIGKMLREVVREKRRRIEEE E EECELLLSRMVR
Sbjct: 196 VLEGVFALPFSFPGTRFSRAKKARMEIGKMLREVVREKRRRIEEELEEEECELLLSRMVR 255

Query: 268 AMIRGEISEVEVVDNVVLLVFAAHDTTSFAIAVTFKMLAQQPNCYSLLLQEHADIVRSKG 327
           AMIRGEISEVEVVDNVVLLVFAAHDTTSFAIA+TFKMLAQ P+CYSLLLQEHADIVRSKG
Sbjct: 256 AMIRGEISEVEVVDNVVLLVFAAHDTTSFAIAMTFKMLAQHPSCYSLLLQEHADIVRSKG 315

Query: 328 PGQNLTMEDIKKMKYTWQVARESIRLFPPIFGSFRKAITDIEYEGFFIPKGWKVLWTTYG 387
            GQNLTMEDIKKMKYTWQVARESIRLFPPIFGSFRKAITDIEYEGFFIPKGWKVLWT YG
Sbjct: 316 AGQNLTMEDIKKMKYTWQVARESIRLFPPIFGSFRKAITDIEYEGFFIPKGWKVLWTMYG 375

Query: 388 THLKDEYFEDPLSFKPSRFEEPVSQYVYVPFGGGPRACAGYQLAKLNILIFIHLVVTRYD 447
           THL+DEYFEDPLSFKPSRFEEPVSQYVYVPFGGGPRACAGYQLAKLNILIFIHLVVTRYD
Sbjct: 376 THLEDEYFEDPLSFKPSRFEEPVSQYVYVPFGGGPRACAGYQLAKLNILIFIHLVVTRYD 435

Query: 448 WSLIHPDEPIIMDPLPIPSHGMPIKISPKSESHPNVQQM 487
           WSLIHPDE IIMDPLPIPSHGM  KISPKSE  PNVQQM
Sbjct: 436 WSLIHPDETIIMDPLPIPSHGMQTKISPKSEVRPNVQQM 474

[Close]

BLAST of Carg15690 vs. TAIR 10
Match: AT2G42850.1 (cytochrome P450, family 718 )

HSP 1 Score: 605.9 bits (1561), Expect = 2.8e-173
Identity = 297/477 (62.26%), Postives = 374/477 (78.41%), Query Frame = 0


Query: 8   FLLATMFLFLSAILIISIV--------FVFRSIKGSKKVPPGQMGLPWIGETMAFYRAQR 67
           F+L+ +FL ++A +  ++         F+ + I+  KK+ PG+MGLPWIGETM FY+AQ+
Sbjct: 7   FVLSWVFLCIAATISSTLFFFRKKHHRFITKKIQKKKKLLPGEMGLPWIGETMDFYKAQK 66

Query: 68  KNRLFEEFVGPRVAKYGKIFKTSLMGSPTVVVNGAEANRFFLSNEFKLVVSSWPSASVQL 127
            NR+FE+FV PR+ K+G IFKT +MGSPT+VVNGAEANR  LSNEF LVVSSWPS+SVQL
Sbjct: 67  SNRVFEDFVNPRIIKHGNIFKTRIMGSPTIVVNGAEANRLILSNEFSLVVSSWPSSSVQL 126

Query: 128 MGEECIMQKQGESHRCARGLIAASLASSSLEAMMPKLCTTIQLYLDTKWVGHDIISLYHS 187
           MG  CIM KQGE HR  RG++A SL+   LE+++PKLC T++ + +T+W G + ISLY S
Sbjct: 127 MGMNCIMAKQGEKHRVLRGIVANSLSYIGLESLIPKLCDTVKFHHETEWRGKEEISLYRS 186

Query: 188 AKVLTFTIVFECLMGIKVEPRMLTTFERVLEGVFAPPFSFPGTRFSRAKKARMEIGKMLR 247
           AKVLTFT+VFECL GIKVE  ML  FERVLEGVFA P  FP ++F+RAKKAR+EI   L 
Sbjct: 187 AKVLTFTVVFECLYGIKVEIGMLEVFERVLEGVFALPVEFPCSKFARAKKARLEIETFLV 246

Query: 248 EVVREKRRRIEEEPEGEECELLLSRMVRAMIRGEISEVEVVDNVVLLVFAAHDTTSFAIA 307
             VREKRR +E+E   +    L SR+V  +I+G I+E EVVDN+VLLVFAAHDTTS+A++
Sbjct: 247 GKVREKRREMEKEGAEKPNTTLFSRLVEELIKGVITEEEVVDNMVLLVFAAHDTTSYAMS 306

Query: 308 VTFKMLAQQPNCYSLLLQEHADIVRSKGPGQNLTMEDIKKMKYTWQVARESIRLFPPIFG 367
           +TFKMLAQ P C   LLQEHA I  +KG G+ LT+ED+KKMKY+WQV RE++RL PPIFG
Sbjct: 307 MTFKMLAQHPTCRDTLLQEHAQIKANKGEGEYLTVEDVKKMKYSWQVVRETMRLSPPIFG 366

Query: 368 SFRKAITDIEYEGFFIPKGWKVLWTTYGTHLKDEYFEDPLSFKPSRFEEPVSQYVYVPFG 427
           SFRKA+ DI+Y G+ IPKGWK+LWTTYGTH   E F+DP+SF P+RF++P+  Y Y+PFG
Sbjct: 367 SFRKAVADIDYGGYTIPKGWKILWTTYGTHYNPEIFQDPMSFDPTRFDKPIQAYTYLPFG 426

Query: 428 GGPRACAGYQLAKLNILIFIHLVVTRYDWSLIHPDEPIIMDPLPIPSHGMPIKISPK 477
           GGPR CAG+QLAK++IL+F+H VVT +DWSL++PDE I MDPLP PS GMPIKISPK
Sbjct: 427 GGPRLCAGHQLAKISILVFMHFVVTGFDWSLVYPDETISMDPLPFPSLGMPIKISPK 483

[Close]

BLAST of Carg15690 vs. TAIR 10
Match: AT5G36110.1 (cytochrome P450, family 716, subfamily A, polypeptide 1 )

HSP 1 Score: 307.4 bits (786), Expect = 2.1e-83
Identity = 176/481 (36.59%), Postives = 273/481 (56.76%), Query Frame = 0


Query: 8   FLLATMFLFLSAILIISIVFVFRSIK--GSKKVPPGQMGLPWIGETMAFYRAQRKNRLFE 67
           ++   + LFLS+IL+  ++ + + +       +PPG  GLP IGE+ +F  A R+    E
Sbjct: 2   YMAIMIILFLSSILLSLLLLLRKHLSHFSYPNLPPGNTGLPLIGESFSFLSAGRQGHP-E 61

Query: 68  EFVGPRVAKYGK----IFKTSLMGSPTVVVNGAEANRFFLSNEFKLVVSSWPSASVQLMG 127
           +F+  RV ++      +FKT L GSPT VV GA  N+F  +NE KLVVS WP  SV  + 
Sbjct: 62  KFITDRVRRFSSSSSCVFKTHLFGSPTAVVTGASGNKFLFTNENKLVVSWWPD-SVNKIF 121

Query: 128 EECIMQKQGESHRCARGLIAASLASSSLEAMMPKLCTTIQLYLDTKWVGHDIISLYHSAK 187
              +     E  R  R L++  +   +L   +  +    Q + +T+W   D + ++   K
Sbjct: 122 PSSMQTSSKEEARKLRMLLSQFMKPEALRRYVGVMDEIAQRHFETEWANQDQVIVFPLTK 181

Query: 188 VLTFTIVFECLMGIKVEPR---MLTTFERVLEGVFAPPFSFPGTRFSRAKKARMEIGKML 247
             TF+I     + ++   R   +   F  V  G+F+ P   PGTRF+RA KA   + K +
Sbjct: 182 KFTFSIACRSFLSMEDPARVRQLEEQFNTVAVGIFSIPIDLPGTRFNRAIKASRLLRKEV 241

Query: 248 REVVREKRRRIEEEPEGEECELLLSRMVRAMIRGEISEVEVVDNVVLLVFAAHDTTSFAI 307
             +VR+++  ++     EE + +LS M+  M  GE  + ++ D ++ L+   HDT S   
Sbjct: 242 SAIVRQRKEELKAGKALEEHD-ILSHML--MNIGETKDEDLADKIIGLLIGGHDTASIVC 301

Query: 308 AVTFKMLAQQPNCYSLLLQEHADIVRSKGPGQNLTMEDIKKMKYTWQVARESIRLFPPIF 367
                 LA+ P+ Y  +LQE  +I++ K   + L  EDI+KM+Y+W VA E +R+ PP+ 
Sbjct: 302 TFVVNYLAEFPHVYQRVLQEQKEILKEKKEKEGLRWEDIEKMRYSWNVACEVMRIVPPLS 361

Query: 368 GSFRKAITDIEYEGFFIPKGWKVLWTTYGTHLKDEYFEDPLSFKPSRFE-EPVSQYVYVP 427
           G+FR+AI    ++GF+IPKGWK+ W+   TH+  +YF +P  F+P+RFE      Y YVP
Sbjct: 362 GTFREAIDHFSFKGFYIPKGWKLYWSATATHMNPDYFPEPERFEPNRFEGSGPKPYTYVP 421

Query: 428 FGGGPRACAGYQLAKLNILIFIHLVVTRYDWSLIHPDE-PIIMDPLPIPSHGMPIKISPK 478
           FGGGPR C G + A+L ILIF+H +V R+ W  + P+E  I++DPLPIP  G+PI+I P+
Sbjct: 422 FGGGPRMCPGKEYARLEILIFMHNLVNRFKWEKVFPNENKIVVDPLPIPDKGLPIRIFPQ 477

[Close]

BLAST of Carg15690 vs. TAIR 10
Match: AT2G29090.1 (cytochrome P450, family 707, subfamily A, polypeptide 2 )

HSP 1 Score: 250.4 bits (638), Expect = 3.0e-66
Identity = 154/470 (32.77%), Postives = 246/470 (52.34%), Query Frame = 0


Query: 15  LFLSAILIISIVFVFRSIKGSK----KVPPGQMGLPWIGETMAFYRAQRKNRLFEEFVGP 74
           L    I+++ +V +F+     K    ++PPG MGLP+IGET+  Y     +     F   
Sbjct: 22  LITLTIVVVVVVLLFKWWLHWKEQRLRLPPGSMGLPYIGETLRLYTENPNS-----FFAT 81

Query: 75  RVAKYGKIFKTSLMGSPTVVVNGAEANRFFLSNEFKLVVSSWPSASVQLMGEECIMQKQG 134
           R  KYG IFKT ++G P V+++  EA R  L ++  L   ++P +  +++G E +   QG
Sbjct: 82  RQNKYGDIFKTHILGCPCVMISSPEAARMVLVSKAHLFKPTYPPSKERMIGPEALFFHQG 141

Query: 135 ESHRCARGLIAASLASSSLEAMMPKLCTTIQLYLDTKWVGHDIISLYHSAKVLTFTIVFE 194
             H   + L+ +S   S+L   +  +   + L   + W     I+     K   F +   
Sbjct: 142 PYHSTLKRLVQSSFMPSALRPTVSHI-ELLVLQTLSSWTSQKSINTLEYMKRYAFDVAIM 201

Query: 195 CLMGIKVEPRMLTT----FERVLEGVFAPPFSFPGTRFSRAKKARMEIGKMLREVVREKR 254
              G K EP  +      ++R+  G  + P   PGT F ++ KAR+E+ + LR+V+ EKR
Sbjct: 202 SAFGDKEEPTTIDVIKLLYQRLERGYNSMPLDLPGTLFHKSMKARIELSEELRKVI-EKR 261

Query: 255 RRIEEEPEGEECELLLSRMVRAMIRGEISEVEVVDNVVLLVFAAHDTTSFAIAVTFKMLA 314
           R    E EG    +LL    +   R  +S+ ++ DN++ ++FAA DTT+  +    K L 
Sbjct: 262 RENGRE-EGGLLGVLLG--AKDQKRNGLSDSQIADNIIGVIFAATDTTASVLTWLLKYLH 321

Query: 315 QQPNCYSLLLQEHADI-VRSKGPGQNLTMEDIKKMKYTWQVARESIRLFPPIFGSFRKAI 374
             PN    + +E   I  + K   + ++ ED +KM  T +V +E++R    +  +FR+A+
Sbjct: 322 DHPNLLQEVSREQFSIRQKIKKENRRISWEDTRKMPLTTRVIQETLRAASVLSFTFREAV 381

Query: 375 TDIEYEGFFIPKGWKVLWTTYGTHLKDEYFEDPLSFKPSRFEEPVSQYVYVPFGGGPRAC 434
            D+EY+G+ IPKGWKVL      H   E+F DP  F PSRFE     Y Y+PFG G  +C
Sbjct: 382 QDVEYDGYLIPKGWKVLPLFRRIHHSSEFFPDPEKFDPSRFEVAPKPYTYMPFGNGVHSC 441

Query: 435 AGYQLAKLNILIFIHLVVTRYDWSLIHPDEPIIMDPLPIPSHGMPIKISP 476
            G +LAKL +LI +H + T + W +I  +E I   P P+P  G+PI+++P
Sbjct: 442 PGSELAKLEMLILLHHLTTSFRWEVIGDEEGIQYGPFPVPKKGLPIRVTP 481

[Close]

BLAST of Carg15690 vs. TAIR 10
Match: AT2G29090.2 (cytochrome P450, family 707, subfamily A, polypeptide 2 )

HSP 1 Score: 250.4 bits (638), Expect = 3.0e-66
Identity = 154/470 (32.77%), Postives = 246/470 (52.34%), Query Frame = 0


Query: 15  LFLSAILIISIVFVFRSIKGSK----KVPPGQMGLPWIGETMAFYRAQRKNRLFEEFVGP 74
           L    I+++ +V +F+     K    ++PPG MGLP+IGET+  Y     +     F   
Sbjct: 22  LITLTIVVVVVVLLFKWWLHWKEQRLRLPPGSMGLPYIGETLRLYTENPNS-----FFAT 81

Query: 75  RVAKYGKIFKTSLMGSPTVVVNGAEANRFFLSNEFKLVVSSWPSASVQLMGEECIMQKQG 134
           R  KYG IFKT ++G P V+++  EA R  L ++  L   ++P +  +++G E +   QG
Sbjct: 82  RQNKYGDIFKTHILGCPCVMISSPEAARMVLVSKAHLFKPTYPPSKERMIGPEALFFHQG 141

Query: 135 ESHRCARGLIAASLASSSLEAMMPKLCTTIQLYLDTKWVGHDIISLYHSAKVLTFTIVFE 194
             H   + L+ +S   S+L   +  +   + L   + W     I+     K   F +   
Sbjct: 142 PYHSTLKRLVQSSFMPSALRPTVSHI-ELLVLQTLSSWTSQKSINTLEYMKRYAFDVAIM 201

Query: 195 CLMGIKVEPRMLTT----FERVLEGVFAPPFSFPGTRFSRAKKARMEIGKMLREVVREKR 254
              G K EP  +      ++R+  G  + P   PGT F ++ KAR+E+ + LR+V+ EKR
Sbjct: 202 SAFGDKEEPTTIDVIKLLYQRLERGYNSMPLDLPGTLFHKSMKARIELSEELRKVI-EKR 261

Query: 255 RRIEEEPEGEECELLLSRMVRAMIRGEISEVEVVDNVVLLVFAAHDTTSFAIAVTFKMLA 314
           R    E EG    +LL    +   R  +S+ ++ DN++ ++FAA DTT+  +    K L 
Sbjct: 262 RENGRE-EGGLLGVLLG--AKDQKRNGLSDSQIADNIIGVIFAATDTTASVLTWLLKYLH 321

Query: 315 QQPNCYSLLLQEHADI-VRSKGPGQNLTMEDIKKMKYTWQVARESIRLFPPIFGSFRKAI 374
             PN    + +E   I  + K   + ++ ED +KM  T +V +E++R    +  +FR+A+
Sbjct: 322 DHPNLLQEVSREQFSIRQKIKKENRRISWEDTRKMPLTTRVIQETLRAASVLSFTFREAV 381

Query: 375 TDIEYEGFFIPKGWKVLWTTYGTHLKDEYFEDPLSFKPSRFEEPVSQYVYVPFGGGPRAC 434
            D+EY+G+ IPKGWKVL      H   E+F DP  F PSRFE     Y Y+PFG G  +C
Sbjct: 382 QDVEYDGYLIPKGWKVLPLFRRIHHSSEFFPDPEKFDPSRFEVAPKPYTYMPFGNGVHSC 441

Query: 435 AGYQLAKLNILIFIHLVVTRYDWSLIHPDEPIIMDPLPIPSHGMPIKISP 476
            G +LAKL +LI +H + T + W +I  +E I   P P+P  G+PI+++P
Sbjct: 442 PGSELAKLEMLILLHHLTTSFRWEVIGDEEGIQYGPFPVPKKGLPIRVTP 481

[Close]

BLAST of Carg15690 vs. TAIR 10
Match: AT5G45340.1 (cytochrome P450, family 707, subfamily A, polypeptide 3 )

HSP 1 Score: 244.2 bits (622), Expect = 2.2e-64
Identity = 149/476 (31.30%), Postives = 249/476 (52.31%), Query Frame = 0


Query: 4   EFSCFLLATMFLFLSAILIISIVFVFRSIKGSKKVPPGQMGLPWIGETMAFYRAQRKNRL 63
           +FS   L      L   L+  I  V RS      +PPG MG P++GET   Y +Q  N  
Sbjct: 2   DFSGLFLTLSAAALFLCLLRFIAGVRRSSSTKLPLPPGTMGYPYVGETFQLY-SQDPN-- 61

Query: 64  FEEFVGPRVAKYGKIFKTSLMGSPTVVVNGAEANRFFLSNEFKLVVSSWPSASVQLMGEE 123
              F   +  +YG +FKT ++G P V+++  EA +F L  +  L   ++P++  +++G++
Sbjct: 62  --VFFAAKQRRYGSVFKTHVLGCPCVMISSPEAAKFVLVTKSHLFKPTFPASKERMLGKQ 121

Query: 124 CIMQKQGESHRCARGLIAASLASSSLEAMMPKLCTTIQLYLDTKWVGHDIISLYHSAKVL 183
            I   QG+ H   R L+  +    ++  M+P + +  Q  L++ W G   ++ Y   K  
Sbjct: 122 AIFFHQGDYHSKLRKLVLRAFMPDAIRNMVPHIESIAQESLNS-WDGTQ-LNTYQEMKTY 181

Query: 184 TFTIVFECLMG---IKVEPRMLTTFERVLEGVFAPPFSFPGTRFSRAKKARMEIGKMLRE 243
           TF +    ++G   +     +   +  + +G  + P + PGT F +A KAR E+ ++L  
Sbjct: 182 TFNVALISILGKDEVYYREDLKRCYYILEKGYNSMPINLPGTLFHKAMKARKELAQILAN 241

Query: 244 VVREKRRRIEEEPEGEECELLLSRMVRAMIRGEISEVEVVDNVVLLVFAAHDTTSFAIAV 303
           ++ ++R+           +LL S M     +  +++ ++ DN++ ++FAA DTT+  +  
Sbjct: 242 ILSKRRQN-----PSSHTDLLGSFMED---KAGLTDEQIADNIIGVIFAARDTTASVLTW 301

Query: 304 TFKMLAQQPNCYSLLLQEHADIVRSKGPGQNLTMEDIKKMKYTWQVARESIRLFPPIFGS 363
             K LA  P     + +E   I + K  G++LT ED KKM  T++V +E++R    +  +
Sbjct: 302 ILKYLADNPTVLEAVTEEQMAIRKDKKEGESLTWEDTKKMPLTYRVIQETLRAATILSFT 361

Query: 364 FRKAITDIEYEGFFIPKGWKVLWTTYGTHLKDEYFEDPLSFKPSRFEEPVSQYVYVPFGG 423
           FR+A+ D+EYEG+ IPKGWKVL      H   + F DP  F PSRFE       ++PFG 
Sbjct: 362 FREAVEDVEYEGYLIPKGWKVLPLFRNIHHNADIFSDPGKFDPSRFEVAPKPNTFMPFGS 421

Query: 424 GPRACAGYQLAKLNILIFIHLVVTRYDWSLIHPDEPIIMDPLPIPSHGMPIKISPK 477
           G  +C G +LAKL I + IH + T+Y WS++ P + I   P  +P +G+PI +  K
Sbjct: 422 GIHSCPGNELAKLEISVLIHHLTTKYRWSIVGPSDGIQYGPFALPQNGLPIALERK 462

The following BLAST results are available for this feature:

BLAST of Carg15690 vs. NCBI nr


Match Name	E-value	Identity	Description
KAG7012036.1	2.6e-277	100.00	hypothetical protein SDJN02_26944, partial [Cucurbita argyrosperma subsp. argyro...	[more]
XP_023554746.1	3.6e-271	97.53	taxadiene 5-alpha hydroxylase isoform X2 [Cucurbita pepo subsp. pepo]	[more]
XP_022952282.1	2.0e-269	96.91	taxadiene 5-alpha hydroxylase isoform X2 [Cucurbita moschata]	[more]
XP_023554747.1	1.0e-257	97.17	taxadiene 5-alpha hydroxylase isoform X3 [Cucurbita pepo subsp. pepo]	[more]
XP_022969335.1	3.0e-257	95.36	taxadiene 5-alpha hydroxylase isoform X1 [Cucurbita maxima]	[more]

BLAST of Carg15690 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q6WG30	3.4e-99	41.19	Taxadiene 5-alpha hydroxylase OS=Taxus cuspidata OX=99806 PE=1 SV=2	[more]
Q8W4T9	1.1e-92	38.05	Taxane 13-alpha-hydroxylase OS=Taxus cuspidata OX=99806 GN=CYP725A2 PE=1 SV=1	[more]
Q50EK0	1.3e-90	39.45	Cytochrome P450 716B2 OS=Picea sitchensis OX=3332 GN=CYP716B2 PE=2 SV=1	[more]
Q50EK1	2.9e-90	39.02	Cytochrome P450 716B1 OS=Picea sitchensis OX=3332 GN=CYP716B1 PE=2 SV=1	[more]
Q84KI1	3.2e-89	38.16	Taxoid 14-beta-hydroxylase OS=Taxus cuspidata OX=99806 PE=1 SV=1	[more]

BLAST of Carg15690 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1GJY9	9.6e-270	96.91	taxadiene 5-alpha hydroxylase isoform X2 OS=Cucurbita moschata OX=3662 GN=LOC111...	[more]
A0A6J1HZM9	1.4e-257	95.36	taxadiene 5-alpha hydroxylase isoform X1 OS=Cucurbita maxima OX=3661 GN=LOC11146...	[more]
A0A6J1GK43	2.7e-256	96.51	taxadiene 5-alpha hydroxylase isoform X3 OS=Cucurbita moschata OX=3662 GN=LOC111...	[more]
A0A6J1GJT6	1.8e-255	95.68	taxadiene 5-alpha hydroxylase isoform X1 OS=Cucurbita moschata OX=3662 GN=LOC111...	[more]
A0A6J1HXH8	1.7e-250	94.99	taxadiene 5-alpha hydroxylase isoform X2 OS=Cucurbita maxima OX=3661 GN=LOC11146...	[more]

BLAST of Carg15690 vs. TAIR 10


Match Name	E-value	Identity	Description
AT2G42850.1	2.8e-173	62.26	cytochrome P450, family 718	[more]
AT5G36110.1	2.1e-83	36.59	cytochrome P450, family 716, subfamily A, polypeptide 1	[more]
AT2G29090.1	3.0e-66	32.77	cytochrome P450, family 707, subfamily A, polypeptide 2	[more]
AT2G29090.2	3.0e-66	32.77	cytochrome P450, family 707, subfamily A, polypeptide 2	[more]
AT5G45340.1	2.2e-64	31.30	cytochrome P450, family 707, subfamily A, polypeptide 3	[more]

InterPro

Analysis Name: InterPro Annotations of Silver-seed gourd (SMH-JMG-627) v2
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR001128	Cytochrome P450	PRINTS	PR00385	P450	coord: 289..306 score: 29.7 coord: 416..425 score: 58.89 coord: 345..356 score: 32.64 coord: 425..436 score: 33.52
IPR001128	Cytochrome P450	PFAM	PF00067	p450	coord: 39..461 e-value: 9.2E-59 score: 199.4
IPR002401	Cytochrome P450, E-class, group I	PRINTS	PR00463	EP450I	coord: 72..91 score: 30.74 coord: 384..408 score: 26.15 coord: 425..448 score: 22.5 coord: 415..425 score: 50.41 coord: 278..295 score: 29.52
IPR036396	Cytochrome P450 superfamily	GENE3D	1.10.630.10	Cytochrome P450	coord: 30..476 e-value: 1.5E-110 score: 372.1
IPR036396	Cytochrome P450 superfamily	SUPERFAMILY	48264	Cytochrome P450	coord: 35..475
None	No IPR available	PIRSR	PIRSR000047-1	PIRSR000047-1	coord: 15..457 e-value: 1.8E-12 score: 44.7
None	No IPR available	PANTHER	PTHR24286	CYTOCHROME P450 26	coord: 27..475
None	No IPR available	PANTHER	PTHR24286:SF221	TAXADIENE 5-ALPHA HYDROXYLASE	coord: 27..475
IPR017972	Cytochrome P450, conserved site	PROSITE	PS00086	CYTOCHROME_P450	coord: 418..427

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Carg15690-RA	Carg15690-RA	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
cellular_component	GO:0016021	integral component of membrane
molecular_function	GO:0020037	heme binding
molecular_function	GO:0005506	iron ion binding
molecular_function	GO:0004497	monooxygenase activity
molecular_function	GO:0016705	oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen