Carg15622 (gene) Silver-seed gourd (SMH-JMG-627) v2

Overview
NameCarg15622
Typegene
OrganismCucurbita argyrosperma subsp. argyrosperma cv. SMH-JMG-627 (Silver-seed gourd (SMH-JMG-627) v2)
Descriptionpolygalacturonase At1g48100
LocationCarg_Chr11: 11190676 .. 11193840 (+)
RNA-Seq ExpressionCarg15622
SyntenyCarg15622
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGAAGATGTGTGGGAAGAATTTAAAATGTCTTGCAACTATTGCTTTCATTTGTGTTTTTGTTTGGTCGTTTAGTTTGGAAAATTGCCATGGGAGAGAAGGCAGGCGTTGGGGGGCACATAGAAAAACTGGAGGCTACCGCCACCACCATGTTCATGTGGATAGTGAGGATGTTGTGGGAGCCTCATTAGCAAGTGAAGGTGGTTCTGGTAGTTTTAATGTGTTGGACTTTGGAGCTAAAGGCGATGGAGAGGAAGATGACACCAAGGTGAATTTAGGTTTGCCTATCATTTTCATTACATAGAAATTGTTAGGAATCACGATTCTCCATAGTGGTATGATATTGTCCACTTTGAGCATAAGCTCTCATGGCTTTGCTTTGGGCTTCCCCAAAAGGCGTCGTACCAATAAAGATGTATTCCTTTACTTATAAACTCATGATCTTCCACTAAATTAACCAACATGGACTCACTCCCAATAATCCTCCGCATATAAACTTTTCAGAATTGAAAATTAACTGAAGTACTTGACAATGTATACATACCAGGCATTTCAGGCAGCTTGGGAATCAGCTTGCAATGCAGAGGGATCAATGGTGGAGGTTCCCTCAGGTTATGAATTCCTCGTTGGCCCCATTTCGTTCTCTGGTCCTAATTGCCAACCAAACATTGTGTTTCAGGTCAATATCAATAACCAAACATTGTGTTTCAGGTCAATATCAATACACTGAAGTCTAGTTTGGAAGTTGATTTCAGAAAGTGTTTTTAAGTGCACAGTAGCATGACTTTTTAAAAGGATTCTATCAACTTTATGGGAAACTGTTCTATGAAGAACTTTTCCAGAAAAAATTCTTGATGACATATTTTAAAGTACATCTCCTACATGACATGCAGTTGGATGGCAAAATCATTGCTCCTACAAGCCCTAGTGCTTGGGGTTCAGGTCTCTTGCAATGGATTGAATTCACTAAACTTAAAGGGATAACAGTGAAAGGTACAGGTACTATTGATGGACAAGGCTCCGTTTGGTGGACCGACGACTCGCCCACAGACGATCCTACTGACGAAAATTTGGTATTTTCATAAATCAACTCGGATTAGAAGGGATTTCATATTTTAAGTCACACAGCTTCCTAATCACAGATTTACTCATGCAACAGGACAAGGAAAGCAGTAGCGGAAGACTTCCAAGAACCAAACTAACAGTACAGCCTTGGCTTAAACTTGACAATAATAGAACAAATGGATTAAAGAGAGATTTCTATTTGTGACTCTTATATTCTTGTGCAGGCACTTAGGTTCTACGGAAGTGATGGAGTGACAGTCACTGGAATAACCATCCAAAACAGCCAACAGGCTCACCTGAAATTTGATAGTTGCACATCAGTTCAGGTTTCTTCATTAACCATTTCATCGCCTGGTGACAGCCCAAACACGGACGGAATTCACTTGCAGAACTCCCAGAATGTGATCATTTCGAACAGCAATCTAGCTTGTGGTAATTCCCAATTTTGAGCACTAACTGCAAAGATGGCTTAATTTAGCATAAGAATTTGGCTTTAAAATACAACCCAACTTTACATAGGCAGATAAATAGAAGGTAATCCAATTGGTTAAAAGTCAGAAAGGTGATCTTCAAGCATCAGTCACTTAAAGGCGGTTTTCAATTTCTTTTTTGCTTAACAGTTCAAGTAGAATATGGTCCTAAAACATGATGTTACGGTGCACTTGATTTCCCTGTTCACCAGCCTGTAATTATGAAGGAAGATCTACTTGTTTTGAAAAAGCATGTCATCGATGAAAACTAGTATGGAATTTAAAAAATCCGTAGCATAATGAAACTTACATGAATGAAATCAAACTACAGGGGATGATTGTATATCCATACAAACTGGAAGCTCGTCCATATACATACACGATGTGAACTGTGGACCAGGCCATGGAATTAGCATTGGAGGACTAGGACGTGATCAAACAAAAGCATGTGTTTCCAATGTCACCGTCCGTGACGTCAAACTGCAGAACACAATGAATGGGGTCAGAATAAAAACATGGCAGGTATATAAAACCTTTCTCCCTAATTTTGACTCCCTAAACCCCAATCTTTATTTAGACAAACCTTTTTCAGTTGTATTTGTTGAGGATTGTTGGGAGGGAGTCTCACATAGGCTAATTTAGGAAATGCTCATGGATTTATACGTAAGGAATACATTTTCATTGGTATGAGGCCTTTTGGGGAAGCCCAAAGCAAAATTATGAGAGACTATACTCAAAGTGAATAACATCATACCATTGTGGAGAGTCGTGATTCTTAACATGATATCAGAGCCATGCCCTTAACTTAGCCATGTCAATAGAGTCTTCAAATGTCGAACAAAGAAGTTGCGAGTCTGGAAGGTGTCGAACAAAGGGTGTACTTTGTTCGAGGACTACAGAGAATGAGTCGAGTCTCGATTAAGGAGAGACTACTCGAGGGCTCTATAGGCCTCAAGGGAGGCCTCTATAGTGTACTTTGTTTGAGGGGAGATTATTGAGGATGGTTGGTAGGGAGTCCCACATTGGCTAATTTAGGGAATGATCATGGGTTTACGTAATGGGTTTATAAGTAAAGAATACATCTCCATTGGTAAGAGGCTTTTTGGAGAAGCCCAAAGCAAAACCATGAAAGCCTATGCTCAAAGTAGACAATATCAGACCATTGTGAAGAGTCGTGATTCTTAGCAATATTGTTTGCCAGAACCCCCTTTTCAATTCAACAGCTCACACAAGGCCATTTAACTTTCCCATTACAGGGTGGTTCAGGCTCAGTACAGGGAATACTGTTCTCAAACATTCAAGTTTCAGACGTTGGGACTCCAGTAATGATAGACCAATACTACTGCGATGGGGGTAGATGCCATAATGGATCTTCTGCAGTAGCAATTTCAGGAGTAAACTATGTAAACATTAGGGGAACATACACTTCAACGCCGGTCCATTTAGCCTGCAGTGACAGTCTTCCCTGCACTGGTGTGACATTAGACACCATTCAGCTCGAGGGGAGCAGCCGCTCAAATGAACCATTCTGTTGGAAGGCATACGGTGAACTGAAAACCTCCACTGTCCCTCCCATCGACTGCTTGCAGTCTGGAAATCCATCGAAAGCCCAGGCTCACGGTTTCTGCTAA

mRNA sequence

ATGAAGATGTGTGGGAAGAATTTAAAATGTCTTGCAACTATTGCTTTCATTTGTGTTTTTGTTTGGTCGTTTAGTTTGGAAAATTGCCATGGGAGAGAAGGCAGGCGTTGGGGGGCACATAGAAAAACTGGAGGCTACCGCCACCACCATGTTCATGTGGATAGTGAGGATGTTGTGGGAGCCTCATTAGCAAGTGAAGGTGGTTCTGTACATCTCCTACATGACATGCAGTTGGATGGCAAAATCATTGCTCCTACAAGCCCTAGTGCTTGGGGTTCAGGTCTCTTGCAATGGATTGAATTCACTAAACTTAAAGGGATAACAGTGAAAGGTACAGGTACTATTGATGGACAAGGCTCCGTTTGGTGGACCGACGACTCGCCCACAGACGATCCTACTGACGAAAATTTGGCACTTAGGTTCTACGGAAGTGATGGAGTGACAGTCACTGGAATAACCATCCAAAACAGCCAACAGGCTCACCTGAAATTTGATAGTTGCACATCAGTTTCTTCATTAACCATTTCATCGCCTGGTGACAGCCCAAACACGGACGGAATTCACTTGCAGAACTCCCAGAATGTGATCATTTCGAACAGCAATCTAGCTTGTGACGTTGGGACTCCAGTAATGATAGACCAATACTACTGCGATGGGGGTAGATGCCATAATGGATCTTCTGCAGTAGCAATTTCAGGAGTAAACTATGTAAACATTAGGGGAACATACACTTCAACGCCGGTCCATTTAGCCTGCAGTGACAGTCTTCCCTGCACTGGTGTGACATTAGACACCATTCAGCTCGAGGGGAGCAGCCGCTCAAATGAACCATTCTGTTGGAAGGCATACGGTGAACTGAAAACCTCCACTGTCCCTCCCATCGACTGCTTGCAGTCTGGAAATCCATCGAAAGCCCAGGCTCACGGTTTCTGCTAA

Coding sequence (CDS)

ATGAAGATGTGTGGGAAGAATTTAAAATGTCTTGCAACTATTGCTTTCATTTGTGTTTTTGTTTGGTCGTTTAGTTTGGAAAATTGCCATGGGAGAGAAGGCAGGCGTTGGGGGGCACATAGAAAAACTGGAGGCTACCGCCACCACCATGTTCATGTGGATAGTGAGGATGTTGTGGGAGCCTCATTAGCAAGTGAAGGTGGTTCTGTACATCTCCTACATGACATGCAGTTGGATGGCAAAATCATTGCTCCTACAAGCCCTAGTGCTTGGGGTTCAGGTCTCTTGCAATGGATTGAATTCACTAAACTTAAAGGGATAACAGTGAAAGGTACAGGTACTATTGATGGACAAGGCTCCGTTTGGTGGACCGACGACTCGCCCACAGACGATCCTACTGACGAAAATTTGGCACTTAGGTTCTACGGAAGTGATGGAGTGACAGTCACTGGAATAACCATCCAAAACAGCCAACAGGCTCACCTGAAATTTGATAGTTGCACATCAGTTTCTTCATTAACCATTTCATCGCCTGGTGACAGCCCAAACACGGACGGAATTCACTTGCAGAACTCCCAGAATGTGATCATTTCGAACAGCAATCTAGCTTGTGACGTTGGGACTCCAGTAATGATAGACCAATACTACTGCGATGGGGGTAGATGCCATAATGGATCTTCTGCAGTAGCAATTTCAGGAGTAAACTATGTAAACATTAGGGGAACATACACTTCAACGCCGGTCCATTTAGCCTGCAGTGACAGTCTTCCCTGCACTGGTGTGACATTAGACACCATTCAGCTCGAGGGGAGCAGCCGCTCAAATGAACCATTCTGTTGGAAGGCATACGGTGAACTGAAAACCTCCACTGTCCCTCCCATCGACTGCTTGCAGTCTGGAAATCCATCGAAAGCCCAGGCTCACGGTTTCTGCTAA

Protein sequence

MKMCGKNLKCLATIAFICVFVWSFSLENCHGREGRRWGAHRKTGGYRHHHVHVDSEDVVGASLASEGGSVHLLHDMQLDGKIIAPTSPSAWGSGLLQWIEFTKLKGITVKGTGTIDGQGSVWWTDDSPTDDPTDENLALRFYGSDGVTVTGITIQNSQQAHLKFDSCTSVSSLTISSPGDSPNTDGIHLQNSQNVIISNSNLACDVGTPVMIDQYYCDGGRCHNGSSAVAISGVNYVNIRGTYTSTPVHLACSDSLPCTGVTLDTIQLEGSSRSNEPFCWKAYGELKTSTVPPIDCLQSGNPSKAQAHGFC
Homology
[Close]
BLAST of Carg15622 vs. NCBI nr
Match: KAG6588952.1 (Polygalacturonase, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 580.1 bits (1494), Expect = 1.1e-161
Identity = 302/396 (76.26%), Postives = 305/396 (77.02%), Query Frame = 0 

Query: 1   MKMCGKNLKCLATIAFICVFVWSFSLENCHGREGRRWGAHRKTGGYRHHHVHVDSEDVVG 60
           MKMCGKNLKCLATIAFICVFVWSFSLENCHGREGRRWGAHRKTGGYRHHHVHVDSEDVVG
Sbjct: 1   MKMCGKNLKCLATIAFICVFVWSFSLENCHGREGRRWGAHRKTGGYRHHHVHVDSEDVVG 60

Query: 61  ASLASEG--GSVHLLH-------------------------------------------- 120
           ASLASEG  GS ++L                                             
Sbjct: 61  ASLASEGGSGSFNVLDFGAKGDGEEDDTKAFQAAWESACNAEGSMVEVPSGYEFLVGPIS 120

Query: 121 ----------DMQLDGKIIAPTSPSAWGSGLLQWIEFTKLKGITVKGTGTIDGQGSVWWT 180
                       QLDGKIIAPTSPSAWGSGLLQWIEFTKLKGITVKGTGTIDGQGSVWWT
Sbjct: 121 FSGPNCQPNIVFQLDGKIIAPTSPSAWGSGLLQWIEFTKLKGITVKGTGTIDGQGSVWWT 180

Query: 181 DDSPTDDPTDENL---------------ALRFYGSDGVTVTGITIQNSQQAHLKFDSCTS 240
           DDSPTDDPTDENL               ALRFYGSDGVTVTGITIQNSQQAHLKFDSCTS
Sbjct: 181 DDSPTDDPTDENLDKESSSGRLPRTKPTALRFYGSDGVTVTGITIQNSQQAHLKFDSCTS 240

Query: 241 --VSSLTISSPGDSPNTDGIHLQNSQNVIISNSNLAC--------------DVGTPVMID 300
             VSSLTISSPGDSPNTDGIHLQNSQNV+ISNSNLAC              DVGTPVMID
Sbjct: 241 VQVSSLTISSPGDSPNTDGIHLQNSQNVVISNSNLACGSVQGILFSNIQVSDVGTPVMID 300

Query: 301 QYYCDGGRCHNGSSAVAISGVNYVNIRGTYTSTPVHLACSDSLPCTGVTLDTIQLEGSSR 310
           QYYCDGGRCHNGSSAVAISGVNYVNIRGTYTSTPVHLACSDSLPCTGVTLDTIQLEGSSR
Sbjct: 301 QYYCDGGRCHNGSSAVAISGVNYVNIRGTYTSTPVHLACSDSLPCTGVTLDTIQLEGSSR 360

[Close]
BLAST of Carg15622 vs. NCBI nr
Match: XP_022928201.1 (polygalacturonase At1g48100 [Cucurbita moschata])

HSP 1 Score: 560.8 bits (1444), Expect = 7.2e-156
Identity = 305/465 (65.59%), Postives = 307/465 (66.02%), Query Frame = 0 

Query: 1   MKMCGKNLKCLATIAFICVFVWSFSLENCHGREGRRWGAHRKTGGYRHHHVHVDSEDVVG 60
           MKMCGKNLKCLATIAFICVFVWSFSLENCHGREGRRWGAHRKTGGYRHHHVHVDSEDVVG
Sbjct: 1   MKMCGKNLKCLATIAFICVFVWSFSLENCHGREGRRWGAHRKTGGYRHHHVHVDSEDVVG 60

Query: 61  ASLASEG--GSVHLLH-------------------------------------------- 120
           ASLASEG  GS ++L                                             
Sbjct: 61  ASLASEGGSGSFNVLDFGAKGDGEEDDTKAFQAAWESACNAEGSMVEVPSGYEFLVGPIS 120

Query: 121 ----------DMQLDGKIIAPTSPSAWGSGLLQWIEFTKLKGITVKGTGTIDGQGSVWWT 180
                       QLDGKIIAPTSPSAWGSGLLQWIEFTKLKGITVKGTGTIDGQGSVWWT
Sbjct: 121 FSGPNCQPNIVFQLDGKIIAPTSPSAWGSGLLQWIEFTKLKGITVKGTGTIDGQGSVWWT 180

Query: 181 DDSPTDDPTDENL---------------ALRFYGSDGVTVTGITIQNSQQAHLKFDSCTS 240
           DDSPTDDPTDENL               ALRFYGSDGVTVTGITIQNSQQAHLKFDSCTS
Sbjct: 181 DDSPTDDPTDENLDKESSSGRLPRTKLTALRFYGSDGVTVTGITIQNSQQAHLKFDSCTS 240

Query: 241 --VSSLTISSPGDSPNTDGIHLQNSQNVIISNSNLAC----------------------- 300
             VSSLTISSPGDSPNTDGIHLQNSQNVIISNSNLAC                       
Sbjct: 241 VPVSSLTISSPGDSPNTDGIHLQNSQNVIISNSNLACGDDCISIQTGSSSIYIHDVNCGP 300

Query: 301 ----------------------------------------------------------DV 312
                                                                     DV
Sbjct: 301 GHGISIGGLGRDQTKACVSNVTVRDVKLQNTMNGVRIKTWQGGSGSVQGILFSNIQVSDV 360

[Close]
BLAST of Carg15622 vs. NCBI nr
Match: XP_023530504.1 (polygalacturonase At1g48100 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 552.7 bits (1423), Expect = 2.0e-153
Identity = 301/465 (64.73%), Postives = 306/465 (65.81%), Query Frame = 0 

Query: 1   MKMCGKNLKCLATIAFICVFVWSFSLENCHGREGRRWGAHRKTGGYRHHHVHVDSEDVVG 60
           MKMCGKNLKCLATIAFICVFVWSFSLENCHGREGRRWGAHRKTGGYRHHHVHVDSEDVVG
Sbjct: 1   MKMCGKNLKCLATIAFICVFVWSFSLENCHGREGRRWGAHRKTGGYRHHHVHVDSEDVVG 60

Query: 61  ASLASEGGS--------------------------------------VHLLHD------- 120
           ASLASEGGS                                      V   ++       
Sbjct: 61  ASLASEGGSGSFNVVDFGAKGDGKEDDTKAFQAAWESACNAEGSMVEVPSGYEFLVGPIS 120

Query: 121 -----------MQLDGKIIAPTSPSAWGSGLLQWIEFTKLKGITVKGTGTIDGQGSVWWT 180
                       QLDG+IIAPTSPSAWGSGLLQWIEFTKLKGITVKGTGTIDGQGSVWW+
Sbjct: 121 FSGPNCQPNIVFQLDGRIIAPTSPSAWGSGLLQWIEFTKLKGITVKGTGTIDGQGSVWWS 180

Query: 181 DDSPTDDPTDENL---------------ALRFYGSDGVTVTGITIQNSQQAHLKFDSCTS 240
           D+SPTDD TDENL               ALRFYGSDGVTVTGITIQNSQQAHLKFDSCTS
Sbjct: 181 DNSPTDDSTDENLDKESSGGRLPRTKPTALRFYGSDGVTVTGITIQNSQQAHLKFDSCTS 240

Query: 241 --VSSLTISSPGDSPNTDGIHLQNSQNVIISNSNLAC----------------------- 300
             VSSLTISSPGDSPNTDGIHLQNSQNVIISNSNLAC                       
Sbjct: 241 VQVSSLTISSPGDSPNTDGIHLQNSQNVIISNSNLACGDDCISIQTGSSSIYIHDVNCGP 300

Query: 301 ----------------------------------------------------------DV 312
                                                                     DV
Sbjct: 301 GHGISIGGLGRDQTKACVSNVTVRDVKLQNTMNGVRIKTWQGGSGSVQGILFSNIQVSDV 360

[Close]
BLAST of Carg15622 vs. NCBI nr
Match: XP_022989159.1 (polygalacturonase At1g48100 [Cucurbita maxima])

HSP 1 Score: 549.7 bits (1415), Expect = 1.7e-152
Identity = 298/465 (64.09%), Postives = 304/465 (65.38%), Query Frame = 0 

Query: 1   MKMCGKNLKCLATIAFICVFVWSFSLENCHGREGRRWGAHRKTGGYRHHHVHVDSEDVVG 60
           MKMCGKNLKCLATIAFIC+FVWSFSLENCHGREGRRWGAHRKTGGYRHHHVHV+SEDVVG
Sbjct: 1   MKMCGKNLKCLATIAFICIFVWSFSLENCHGREGRRWGAHRKTGGYRHHHVHVESEDVVG 60

Query: 61  ASLASEG--GSVHLLH-------------------------------------------- 120
           +SLASEG  GS ++L                                             
Sbjct: 61  SSLASEGGSGSFNVLDFGAKGDGEEDDTKAFQAAWESACNAEGSMVEVPSGYEFLVGPIS 120

Query: 121 ----------DMQLDGKIIAPTSPSAWGSGLLQWIEFTKLKGITVKGTGTIDGQGSVWWT 180
                       QLDGKIIAPTSPSAWGSGLLQWIEFTKLKGITVKGTGTIDGQGSVWW+
Sbjct: 121 FSGPNCQPNIVFQLDGKIIAPTSPSAWGSGLLQWIEFTKLKGITVKGTGTIDGQGSVWWS 180

Query: 181 DDSPTDDPTDENL---------------ALRFYGSDGVTVTGITIQNSQQAHLKFDSCTS 240
           DDSPTDDPTDENL               ALRFYGSDGVTVTGITIQNSQQAHLKFDSCTS
Sbjct: 181 DDSPTDDPTDENLDKESSSGRLPRTKPTALRFYGSDGVTVTGITIQNSQQAHLKFDSCTS 240

Query: 241 --VSSLTISSPGDSPNTDGIHLQNSQNVIISNSNLAC----------------------- 300
             VSSLTISSPGDSPNTDGIHLQNSQNVIISNSNLAC                       
Sbjct: 241 VQVSSLTISSPGDSPNTDGIHLQNSQNVIISNSNLACGDDCISIQTGSSSIYIHDVNCGP 300

Query: 301 ----------------------------------------------------------DV 312
                                                                     DV
Sbjct: 301 GHGISIGGLGRDQTKACVSNVTVRDVKLQNTMNGVRIKTWQGGSGSVLGILFSNIQVSDV 360

[Close]
BLAST of Carg15622 vs. NCBI nr
Match: KAG7017769.1 (Polygalacturonase, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 488.4 bits (1256), Expect = 4.5e-134
Identity = 278/471 (59.02%), Postives = 288/471 (61.15%), Query Frame = 0 

Query: 1   MKMCGKNLKCLATIAFICVFVWSFSLENCHGREGRRWGAHRKTGGYRHHHV--------- 60
           M+M GKNLKCLATIAFIC FVWSFSL+NC  REG+ W AH KTGGYRH  +         
Sbjct: 1   MEMGGKNLKCLATIAFICAFVWSFSLQNCQAREGKHW-AHGKTGGYRHGRIHRDRGGSGS 60

Query: 61  -------HVDSEDVVGASLASEG------------------------------------- 120
                  HV SEDVVGASLAS+G                                     
Sbjct: 61  SRLTGSGHVGSEDVVGASLASKGDASKGGSGNFNVLDFGAKGDGRADDTKAFQAAWESAC 120

Query: 121 -----------GSVHLLHDM-------------QLDGKIIAPTSPSAWGSGLLQWIEFTK 180
                      GS  L+  +             QLDGKIIAPTSPSAWGSGLLQWIEFTK
Sbjct: 121 SAEKSTVEVPSGSEFLVGPISFSGPNCKPNIVFQLDGKIIAPTSPSAWGSGLLQWIEFTK 180

Query: 181 LKGITVKGTGTIDGQGSVWWTDDSPTDDPTDENLALRFYGSDGVTVTGITIQNSQQAHLK 240
           LKGITVKGTGTIDGQGSVWW +DSPT DPTDENLALRFYGSDGVTV+GITIQNSQQAHLK
Sbjct: 181 LKGITVKGTGTIDGQGSVWW-NDSPTYDPTDENLALRFYGSDGVTVSGITIQNSQQAHLK 240

Query: 241 FDSCTS--VSSLTISSPGDSPNTDGIHLQNSQNVIISNSNLAC----------------- 300
           FDSCTS  VSSLTISSPGDSPNTDGIHLQNSQNVIISNSNLAC                 
Sbjct: 241 FDSCTSVQVSSLTISSPGDSPNTDGIHLQNSQNVIISNSNLACGDDCISIQTGSSAVYIH 300

Query: 301 ------------------------------------------------------------ 312
                                                                       
Sbjct: 301 NVNCGPGHGISIGGLGRDQTKACVSNVTVRDVKLQNTMNGVRIKTWQGGSGLVQGILFSN 360

[Close]
BLAST of Carg15622 vs. ExPASy Swiss-Prot
Match: Q949Z1 (Polygalacturonase At1g48100 OS=Arabidopsis thaliana OX=3702 GN=At1g48100 PE=2 SV=1)

HSP 1 Score: 174.5 bits (441), Expect = 1.9e-42
Identity = 111/358 (31.01%), Postives = 156/358 (43.58%), Query Frame = 0 

Query: 60  GASLASEGGSVHLLHDM-----------QLDGKIIAPTSPSAW--GSGLLQWIEFTKLKG 119
           G  LA EGG   +   +           QLDG ++ P  P  W       QW+ F +L G
Sbjct: 112 GVVLAPEGGVFKITSTIFSGPCKPGLVFQLDGVLMPPDGPEEWPEKDNKNQWLVFYRLDG 171

Query: 120 ITVKGTGTIDGQGSVWW---------TDDSPTDDPTDENLALRFYGSDGVTVTGITIQNS 179
            T  G GT++G G  WW          D S +  P      +RF+ S+ + V G+ IQNS
Sbjct: 172 FTFSGKGTVEGNGQKWWDLPCKPHRGPDGSSSSGPCASPTMIRFFMSNNIEVKGLRIQNS 231

Query: 180 QQAHLKFDSCTSV--SSLTISSPGDSPNTDGIHLQNSQNV-----IISNSNLACDVGTP- 239
            Q H+KFD C  V  + + ISSP  SPNTDGIHL N+++V     ++SN +    +GT  
Sbjct: 232 PQFHMKFDGCQGVLINEIQISSPKLSPNTDGIHLGNTRSVGIYNSVVSNGDDCISIGTGC 291

Query: 240 ------------------------------------------------------------ 299
                                                                       
Sbjct: 292 SDVDIQGVTCGPSHGISIGSLGVHNSQACVSNITVRNTVIRDSDNGLRVKTWQGGTGSVS 351

Query: 300 ---------------VMIDQYYCDGGRCHNGSSAVAISGVNYVNIRGTY--TSTPVHLAC 309
                          +++DQYYC    C N +SAV +  V Y NI+GTY   S P+H AC
Sbjct: 352 NLLFENIQMENVLNCIIVDQYYCQSKDCRNETSAVKVFDVEYRNIKGTYDVRSPPIHFAC 411

[Close]
BLAST of Carg15622 vs. ExPASy Swiss-Prot
Match: Q94AJ5 (Probable polygalacturonase At1g80170 OS=Arabidopsis thaliana OX=3702 GN=At1g80170 PE=1 SV=1)

HSP 1 Score: 131.7 bits (330), Expect = 1.4e-29
Identity = 87/309 (28.16%), Postives = 131/309 (42.39%), Query Frame = 0 

Query: 76  MQLDGKIIAPTSPSAW-GSGLLQWIEFTKLKGITVKGTGTIDGQGSVWW---TDDSPTDD 135
           +Q+ G IIAP  P  W G    +W+ F  L  +TV+G GT++G G  WW      + ++ 
Sbjct: 113 LQISGTIIAPNDPDVWEGLNRRKWLYFHGLSRLTVEGGGTVNGMGQEWWERSCKHNHSNP 172

Query: 136 PTDENLALRFYGSDGVTVTGITIQNSQQAHLKFDSC--TSVSSLTISSPGDSPNTDGIHL 195
                 AL F+    + V  + + +SQQ H+   SC   ++S L + +P  SPNTDGIH+
Sbjct: 173 CRGAPTALTFHKCKNMRVENLNVIDSQQMHIALTSCRRVTISGLKVIAPATSPNTDGIHI 232

Query: 196 QNSQNVIISN---------------------SNLAC------------------------ 255
             S+ ++I N                     SN+ C                        
Sbjct: 233 SVSRGIVIDNTTVSTGDDCISIVKNSTQISISNIICGPGHGISIGSLGKSKSWEEVRDIT 292

Query: 256 ------------------------------------DVGTPVMIDQYYCDGGR-CHNGSS 294
                                               +V  P++IDQYYCD  + C N +S
Sbjct: 293 VDTAIISDTANGVRIKTWQGGSGLVSKIIFRNIKMNNVSNPIIIDQYYCDSRKPCANQTS 352

[Close]
BLAST of Carg15622 vs. ExPASy Swiss-Prot
Match: P35336 (Polygalacturonase OS=Actinidia deliciosa OX=3627 PE=2 SV=1)

HSP 1 Score: 110.2 bits (274), Expect = 4.4e-23
Identity = 79/282 (28.01%), Postives = 118/282 (41.84%), Query Frame = 0 

Query: 76  MQLDGKIIAPTSPSAWGSGLLQWIEFTKLKGITVKGTGTIDGQGSVWWTDDSPTDDP--- 135
           MQ+ G I A    S +      W+ F  ++ + V+G GTI+G G +WW +   T+     
Sbjct: 148 MQIYGTIEASDDRSDYRKDGRHWLVFDSVQNLRVEGGGTINGNGKIWWQNSCKTNKALPC 207

Query: 136 TDENLALRFYGSDGVTVTGITIQNSQQAHLKFDSCTSV--SSLTISSPGDSPNTDGIHLQ 195
            D   AL FY S  V V  + I+N+QQ H+ FD+C +V  S+L +++P +SPNTDGIH+ 
Sbjct: 208 KDAPTALTFYKSKHVIVKNLKIENAQQIHVSFDNCVNVQASNLMVTAPENSPNTDGIHVT 267

Query: 196 NSQNVIISN---------------------SNLAC------------------------- 255
            +QN+ IS+                     +++ C                         
Sbjct: 268 GTQNIHISSCVIGTGDDCISIVNGSRKVRVNDITCGPGHGISIGSLGYGNSEAHVSDVVV 327

Query: 256 -----------------------------------DVGTPVMIDQYYCDGGR-CHNGSSA 270
                                              +V  P++IDQ YCD  + C   SSA
Sbjct: 328 NGAKLCGTTNGVRIKTWQGGSGSASNIKFQNVEMHNVENPIIIDQNYCDQDKPCQEQSSA 387

[Close]
BLAST of Carg15622 vs. ExPASy Swiss-Prot
Match: P43212 (Polygalacturonase OS=Cryptomeria japonica OX=3369 PE=1 SV=1)

HSP 1 Score: 108.2 bits (269), Expect = 1.7e-22
Identity = 83/289 (28.72%), Postives = 120/289 (41.52%), Query Frame = 0 

Query: 77  QLDGKIIAPTSPSAWGSGLLQWIEFTKLKGITVKGTGTIDGQGSVW------WTDDSPTD 136
           ++DG I A  +P++W +  + W++F KL G T+ G G IDGQG  W      W +     
Sbjct: 119 KVDGIIAAYQNPASWKNNRI-WLQFAKLTGFTLMGKGVIDGQGKQWWAGQCKWVNGREIC 178

Query: 137 DPTDENLALRFYGSDGVTVTGITIQNSQQAHLKFDSCTSVS--SLTISSPGDSPNTDGI- 196
           +  D   A++F  S G+ + G+ + NS + HL F +C  V    ++I++P DSPNTDGI 
Sbjct: 179 NDRDRPTAIKFDFSTGLIIQGLKLMNSPEFHLVFGNCEGVKIIGISITAPRDSPNTDGID 238

Query: 197 -------HLQ---------------NSQNVIISNSNLACDVG------------------ 256
                  HLQ                S N++I   +L C  G                  
Sbjct: 239 IFASKNFHLQKNTIGTGDDCVAIGTGSSNIVI--EDLICGPGHGISIGSLGRENSRAEVS 298

Query: 257 ------------------------------------------TPVMIDQYYC-DGGRCHN 273
                                                      P++I+Q+YC     C N
Sbjct: 299 YVHVNGAKFIDTQNGLRIKTWQGGSGMASHIIYENVEMINSENPILINQFYCTSASACQN 358

[Close]
BLAST of Carg15622 vs. ExPASy Swiss-Prot
Match: O23147 (Polygalacturonase ADPG1 OS=Arabidopsis thaliana OX=3702 GN=ADPG1 PE=2 SV=1)

HSP 1 Score: 104.0 bits (258), Expect = 3.1e-21
Identity = 76/296 (25.68%), Postives = 118/296 (39.86%), Query Frame = 0 

Query: 68  GSVHLLHDMQLDGKIIAPTSPSAWGSGLLQWIEFTKLKGITVKG--TGTIDGQGSVWWTD 127
           G    L + Q+ G + A T  S +      W+    +  +++ G  TG I+G G  WW +
Sbjct: 120 GPCKSLRNFQILGTLSASTKRSDY-KDKNHWLILEDVNNLSIDGGSTGIINGNGKTWWQN 179

Query: 128 DSPTDDP---TDENLALRFYGSDGVTVTGITIQNSQQAHLKFDSC--TSVSSLTISSPGD 187
               D     T    AL  Y    + V  + ++N+QQ  +  + C    VS++ I++PGD
Sbjct: 180 SCKIDKSKPCTKAPTALTLYNLKNLNVKNLRVKNAQQIQISIEKCNKVEVSNVEITAPGD 239

Query: 188 SPNTDGIHLQNSQNVIISNS---------------------NLAC--------------- 247
           SPNTDGIH+ N+QN+ +SNS                     +L C               
Sbjct: 240 SPNTDGIHITNTQNIRVSNSDIGTGDDCISIEDGTQNLQIFDLTCGPGHGISIGSLGDDN 299

Query: 248 ---------------------------------------------DVGTPVMIDQYYCDG 275
                                                        +V  P++IDQ YCD 
Sbjct: 300 SKAYVSGINVDGAKFSESDNGVRIKTYQGGSGTAKNIKFQNIRMENVKNPIIIDQDYCDK 359

[Close]
BLAST of Carg15622 vs. ExPASy TrEMBL
Match: A0A6J1EJN2 (polygalacturonase At1g48100 OS=Cucurbita moschata OX=3662 GN=LOC111435099 PE=3 SV=1)

HSP 1 Score: 560.8 bits (1444), Expect = 3.5e-156
Identity = 305/465 (65.59%), Postives = 307/465 (66.02%), Query Frame = 0 

Query: 1   MKMCGKNLKCLATIAFICVFVWSFSLENCHGREGRRWGAHRKTGGYRHHHVHVDSEDVVG 60
           MKMCGKNLKCLATIAFICVFVWSFSLENCHGREGRRWGAHRKTGGYRHHHVHVDSEDVVG
Sbjct: 1   MKMCGKNLKCLATIAFICVFVWSFSLENCHGREGRRWGAHRKTGGYRHHHVHVDSEDVVG 60

Query: 61  ASLASEG--GSVHLLH-------------------------------------------- 120
           ASLASEG  GS ++L                                             
Sbjct: 61  ASLASEGGSGSFNVLDFGAKGDGEEDDTKAFQAAWESACNAEGSMVEVPSGYEFLVGPIS 120

Query: 121 ----------DMQLDGKIIAPTSPSAWGSGLLQWIEFTKLKGITVKGTGTIDGQGSVWWT 180
                       QLDGKIIAPTSPSAWGSGLLQWIEFTKLKGITVKGTGTIDGQGSVWWT
Sbjct: 121 FSGPNCQPNIVFQLDGKIIAPTSPSAWGSGLLQWIEFTKLKGITVKGTGTIDGQGSVWWT 180

Query: 181 DDSPTDDPTDENL---------------ALRFYGSDGVTVTGITIQNSQQAHLKFDSCTS 240
           DDSPTDDPTDENL               ALRFYGSDGVTVTGITIQNSQQAHLKFDSCTS
Sbjct: 181 DDSPTDDPTDENLDKESSSGRLPRTKLTALRFYGSDGVTVTGITIQNSQQAHLKFDSCTS 240

Query: 241 --VSSLTISSPGDSPNTDGIHLQNSQNVIISNSNLAC----------------------- 300
             VSSLTISSPGDSPNTDGIHLQNSQNVIISNSNLAC                       
Sbjct: 241 VPVSSLTISSPGDSPNTDGIHLQNSQNVIISNSNLACGDDCISIQTGSSSIYIHDVNCGP 300

Query: 301 ----------------------------------------------------------DV 312
                                                                     DV
Sbjct: 301 GHGISIGGLGRDQTKACVSNVTVRDVKLQNTMNGVRIKTWQGGSGSVQGILFSNIQVSDV 360

[Close]
BLAST of Carg15622 vs. ExPASy TrEMBL
Match: A0A6J1JNK8 (polygalacturonase At1g48100 OS=Cucurbita maxima OX=3661 GN=LOC111486313 PE=3 SV=1)

HSP 1 Score: 549.7 bits (1415), Expect = 8.0e-153
Identity = 298/465 (64.09%), Postives = 304/465 (65.38%), Query Frame = 0 

Query: 1   MKMCGKNLKCLATIAFICVFVWSFSLENCHGREGRRWGAHRKTGGYRHHHVHVDSEDVVG 60
           MKMCGKNLKCLATIAFIC+FVWSFSLENCHGREGRRWGAHRKTGGYRHHHVHV+SEDVVG
Sbjct: 1   MKMCGKNLKCLATIAFICIFVWSFSLENCHGREGRRWGAHRKTGGYRHHHVHVESEDVVG 60

Query: 61  ASLASEG--GSVHLLH-------------------------------------------- 120
           +SLASEG  GS ++L                                             
Sbjct: 61  SSLASEGGSGSFNVLDFGAKGDGEEDDTKAFQAAWESACNAEGSMVEVPSGYEFLVGPIS 120

Query: 121 ----------DMQLDGKIIAPTSPSAWGSGLLQWIEFTKLKGITVKGTGTIDGQGSVWWT 180
                       QLDGKIIAPTSPSAWGSGLLQWIEFTKLKGITVKGTGTIDGQGSVWW+
Sbjct: 121 FSGPNCQPNIVFQLDGKIIAPTSPSAWGSGLLQWIEFTKLKGITVKGTGTIDGQGSVWWS 180

Query: 181 DDSPTDDPTDENL---------------ALRFYGSDGVTVTGITIQNSQQAHLKFDSCTS 240
           DDSPTDDPTDENL               ALRFYGSDGVTVTGITIQNSQQAHLKFDSCTS
Sbjct: 181 DDSPTDDPTDENLDKESSSGRLPRTKPTALRFYGSDGVTVTGITIQNSQQAHLKFDSCTS 240

Query: 241 --VSSLTISSPGDSPNTDGIHLQNSQNVIISNSNLAC----------------------- 300
             VSSLTISSPGDSPNTDGIHLQNSQNVIISNSNLAC                       
Sbjct: 241 VQVSSLTISSPGDSPNTDGIHLQNSQNVIISNSNLACGDDCISIQTGSSSIYIHDVNCGP 300

Query: 301 ----------------------------------------------------------DV 312
                                                                     DV
Sbjct: 301 GHGISIGGLGRDQTKACVSNVTVRDVKLQNTMNGVRIKTWQGGSGSVLGILFSNIQVSDV 360

[Close]
BLAST of Carg15622 vs. ExPASy TrEMBL
Match: A0A6J1F9Z9 (polygalacturonase At1g48100-like OS=Cucurbita moschata OX=3662 GN=LOC111442159 PE=3 SV=1)

HSP 1 Score: 479.2 bits (1232), Expect = 1.3e-131
Identity = 278/486 (57.20%), Postives = 288/486 (59.26%), Query Frame = 0 

Query: 1   MKMCGKNLKCLATIAFICVFVWSFSLENCHGREGRRWGAHRKTGGYRHHHV--------- 60
           M+M GKNLKCLATIAFIC FVWSFSL+NC  REG+ W AHRKTGGYRH  +         
Sbjct: 1   MEMGGKNLKCLATIAFICAFVWSFSLQNCQAREGKHW-AHRKTGGYRHGRIHRDRGGSGS 60

Query: 61  -------HVDSEDVVGASLASEG------------------------------------- 120
                  HV SEDVVGASLAS+G                                     
Sbjct: 61  SRLTGSGHVGSEDVVGASLASKGDASKGGSGNFNVLDFGAKGDGRADDTKAFQAAWESAC 120

Query: 121 -----------GSVHLLHDM-------------QLDGKIIAPTSPSAWGSGLLQWIEFTK 180
                      GS  L+  +             QLDGKIIAPTSPSAWGSGLLQWIEFTK
Sbjct: 121 SAEKSTVEVPSGSEFLVGPISFSGPNCKPNIVFQLDGKIIAPTSPSAWGSGLLQWIEFTK 180

Query: 181 LKGITVKGTGTIDGQGSVWWTDDSPTDDPTDENL---------------ALRFYGSDGVT 240
           LKGITVKGTGTIDGQGSVWW +DSPT DPTDENL               ALRFYGSDGVT
Sbjct: 181 LKGITVKGTGTIDGQGSVWW-NDSPTYDPTDENLDKESSSGKLPSTKPTALRFYGSDGVT 240

Query: 241 VTGITIQNSQQAHLKFDSCTS--VSSLTISSPGDSPNTDGIHLQNSQNVIISNSNLAC-- 300
           V+GITIQNSQQAHLKFDSCTS  VSSLTISSPGDSPNTDGIHLQNSQNVIISNSNLAC  
Sbjct: 241 VSGITIQNSQQAHLKFDSCTSVQVSSLTISSPGDSPNTDGIHLQNSQNVIISNSNLACGD 300

Query: 301 ------------------------------------------------------------ 312
                                                                       
Sbjct: 301 DCISIQTGSSAVYIHNVNCGPGHGISIGGLGRDQTKACVSNVTVRDVKLQNTMNGVRIKT 360

[Close]
BLAST of Carg15622 vs. ExPASy TrEMBL
Match: A0A6J1J1T6 (polygalacturonase At1g48100-like OS=Cucurbita maxima OX=3661 GN=LOC111481937 PE=3 SV=1)

HSP 1 Score: 476.1 bits (1224), Expect = 1.1e-130
Identity = 276/486 (56.79%), Postives = 288/486 (59.26%), Query Frame = 0 

Query: 1   MKMCGKNLKCLATIAFICVFVWSFSLENCHGREGRRWGAHRKTGGYRHHHV--------- 60
           M+M GKNLKCLATIAFIC FVWSFSL+NC  R G+ W AHRKTGGYRH  +         
Sbjct: 1   MEMGGKNLKCLATIAFICAFVWSFSLQNCQARGGKHW-AHRKTGGYRHGRIHRHRGGSSS 60

Query: 61  -------HVDSEDVVGASLASEG------------------------------------- 120
                  HV SEDVVGASLAS+G                                     
Sbjct: 61  LGLTDSGHVGSEDVVGASLASKGDASKGGSGNFNVLDFGAKGDGRADDTKAFQAAWESAC 120

Query: 121 -----------GSVHLLHDM-------------QLDGKIIAPTSPSAWGSGLLQWIEFTK 180
                      GS  L+  +             QLDGKIIAPTSPSAWGSGLLQWIEFTK
Sbjct: 121 NAEKSTVEVPSGSEFLVGPISFSGPNCKPNIVFQLDGKIIAPTSPSAWGSGLLQWIEFTK 180

Query: 181 LKGITVKGTGTIDGQGSVWWTDDSPTDDPTDENL---------------ALRFYGSDGVT 240
           LKGITVKGTGTIDGQGSVWW +DSPT DPTDENL               ALRFYGSDGVT
Sbjct: 181 LKGITVKGTGTIDGQGSVWW-NDSPTYDPTDENLDKESSSRKLPSTKPTALRFYGSDGVT 240

Query: 241 VTGITIQNSQQAHLKFDSCTS--VSSLTISSPGDSPNTDGIHLQNSQNVIISNSNLAC-- 300
           V+GITIQNSQQAHLKFDSCTS  VSSLTISSPGDSPNTDGIHLQNSQNVIISNSNLAC  
Sbjct: 241 VSGITIQNSQQAHLKFDSCTSVQVSSLTISSPGDSPNTDGIHLQNSQNVIISNSNLACGD 300

Query: 301 ------------------------------------------------------------ 312
                                                                       
Sbjct: 301 DCISIQTGSSAVYIHNVNCGPGHGISIGGLGRDQTKACVSNVTVRDVKLQNTMNGVRIKT 360

[Close]
BLAST of Carg15622 vs. ExPASy TrEMBL
Match: A0A1S3B8B8 (polygalacturonase At1g48100 OS=Cucumis melo OX=3656 GN=LOC103487102 PE=3 SV=1)

HSP 1 Score: 444.1 bits (1141), Expect = 4.7e-121
Identity = 262/478 (54.81%), Postives = 276/478 (57.74%), Query Frame = 0 

Query: 1   MKMCGKNLKCLATIAFICVFVWSFSLENCHGREG---RRWGAHRKTGGYRHH-----HVH 60
           M+M  KNLKCL TIAFICVFVWSFSL NCH R+    R W  HR   G           H
Sbjct: 1   MEMGRKNLKCLTTIAFICVFVWSFSLHNCHARKTVGYRHWQVHRHHQGSGRSLRLTGSGH 60

Query: 61  VDSEDVVGASLASEG--------------------------------------------- 120
           V SEDVVG SLAS+G                                             
Sbjct: 61  VGSEDVVGTSLASKGRATNGGSGNFNVLDFGAKGDGETDDTKAFQSAWESACNVEGSVVE 120

Query: 121 ---GSVHLLHDM-------------QLDGKIIAPTSPSAWGSGLLQWIEFTKLKGITVKG 180
              GS  L+  +             QLDGKIIAPTSPSAWGSG+LQWIEFTKLKGITVKG
Sbjct: 121 VPSGSNFLVGPISFSGPNCQPNIMFQLDGKIIAPTSPSAWGSGILQWIEFTKLKGITVKG 180

Query: 181 TGTIDGQGSVWWTDDSPTDDPTDENL---------------ALRFYGSDGVTVTGITIQN 240
           TGTIDGQGSVWW +DSPT++PTDENL               ALRFYGSD VTV+GITIQN
Sbjct: 181 TGTIDGQGSVWW-NDSPTNNPTDENLDKESSGGKLPSTKPTALRFYGSDEVTVSGITIQN 240

Query: 241 SQQAHLKFDSCTS--VSSLTISSPGDSPNTDGIHLQNSQNVIISNSNLAC---------- 300
           SQ+AHLKFDSCT+  VSSLTISSPGDSPNTDGIHLQNSQNVIISNSNLAC          
Sbjct: 241 SQKAHLKFDSCTAVQVSSLTISSPGDSPNTDGIHLQNSQNVIISNSNLACGDDCISIQTG 300

Query: 301 ------------------------------------------------------------ 312
                                                                       
Sbjct: 301 SSGVYIHNVNCGPGHGISIGGLGRDETKACVSNITVRDVKLQNTMNGVRIKTWQGGSGSV 360

[Close]
BLAST of Carg15622 vs. TAIR 10
Match: AT5G14650.1 (Pectin lyase-like superfamily protein )

HSP 1 Score: 293.1 bits (749), Expect = 2.6e-79
Identity = 156/315 (49.52%), Postives = 185/315 (58.73%), Query Frame = 0 

Query: 77  QLDGKIIAPTSPSAWGSGLLQWIEFTKLKGITVKGTGTIDGQGSVWWTDDSPTDDPTDEN 136
           QL+GKIIAPTS SAWGSGLLQWIEF  L+GIT+KG G IDG+GSVWW D   T  P  + 
Sbjct: 112 QLEGKIIAPTSASAWGSGLLQWIEFKALQGITIKGKGIIDGRGSVWWNDMMGTKMPRTKP 171

Query: 137 LALRFYGSDGVTVTGITIQNSQQAHLKFDSCTS--VSSLTISSPGDSPNTDGIHLQNSQN 196
            ALRFYGS+GVTV+GITIQNS Q HLKFD+C S  VS  T SSPGDSPNTDGIHLQNSQ+
Sbjct: 172 TALRFYGSNGVTVSGITIQNSPQTHLKFDNCISIQVSDFTTSSPGDSPNTDGIHLQNSQD 231

Query: 197 VIISNSNLAC-------------------------------------------------- 256
            +I  S LAC                                                  
Sbjct: 232 AVIYRSTLACGDDCISIQTGCSNINIHDVDCGPGHGISIGGLGKDNTKACVSNITVRDVT 291

Query: 257 -------------------------------DVGTPVMIDQYYCDGGRCHNGSSAVAISG 306
                                          +V  P++IDQYYCDGG CHN +SAVA+S 
Sbjct: 292 MHETTNGVRIKSWQGGSGSVKQVMFSNIQVSNVANPIIIDQYYCDGGGCHNETSAVAVSN 351

[Close]
BLAST of Carg15622 vs. TAIR 10
Match: AT1G60590.1 (Pectin lyase-like superfamily protein )

HSP 1 Score: 243.0 bits (619), Expect = 3.1e-64
Identity = 137/350 (39.14%), Postives = 172/350 (49.14%), Query Frame = 0 

Query: 77  QLDGKIIAPTSPSAWGSGLLQWIEFTKLKGITVKGTGTIDGQGSVWWTDDSPTDDPTDEN 136
           QL+G I+APT   +WG GL+ WIEFTKL GIT++G G IDG+G+VWW  D  +D P D++
Sbjct: 184 QLEGMIVAPTDTESWGGGLMWWIEFTKLSGITIQGNGVIDGRGTVWWQQDYLSDYPIDDD 243

Query: 137 L--------------------------------ALRFYGSDGVTVTGITIQNSQQAHLKF 196
                                            ALRFYGS  VTVTGITIQNS Q HLKF
Sbjct: 244 FKLIVPLNNSVQERPPMPIRSELNWRMPSIKPTALRFYGSIDVTVTGITIQNSPQCHLKF 303

Query: 197 DSCTS--VSSLTISSPGDSPNTDGIHLQNSQNVIISNSNLAC------------------ 256
           D+C    V  + +SSPGDSPNTDGIHLQN+++V+I  + LAC                  
Sbjct: 304 DNCVKVLVHDVNVSSPGDSPNTDGIHLQNTRDVMIHTTTLACGDDCISIQTGCSNVYVHN 363

Query: 257 ------------------------------------------------------------ 309
                                                                       
Sbjct: 364 VNCGPGHGISIGSLGKDSTKACVSNITVRDVVMHNTMTGVRIKTWQGGIGSVKGILFSNI 423

[Close]
BLAST of Carg15622 vs. TAIR 10
Match: AT1G10640.1 (Pectin lyase-like superfamily protein )

HSP 1 Score: 230.7 bits (587), Expect = 1.6e-60
Identity = 137/353 (38.81%), Postives = 170/353 (48.16%), Query Frame = 0 

Query: 77  QLDGKIIAPTSPSAWGSGLLQWIEFTKLKGITVKGTGTIDGQGSVWWTDDSPTDD----- 136
           QLDG IIAPT    WG GL+ WI+FTKLKGI V+G G IDG+GS WW  DSP  D     
Sbjct: 180 QLDGTIIAPTDSKTWGKGLMWWIDFTKLKGIKVQGKGVIDGRGSGWWQQDSPFIDSDTKL 239

Query: 137 ------------------------PTDENLALRFYGSDGVTVTGITIQNSQQAHLKFDSC 196
                                   P+ +  ALRF GS GV VTGITIQNS Q HLKFD C
Sbjct: 240 IVPLNNSANQNPPMPIRSELDERMPSIKPTALRFSGSFGVEVTGITIQNSPQCHLKFDDC 299

Query: 197 TS--VSSLTISSPGDSPNTDGIHLQNSQNVIISNSNLAC--------------------- 256
               V  + +SSPGDSPNTDGIHLQN+++V+I ++ LAC                     
Sbjct: 300 VGVVVHDIAVSSPGDSPNTDGIHLQNTKDVLIHSTTLACGDDCISIQTGCSNVFVHNVNC 359

Query: 257 ------------------------------------------------------------ 312
                                                                       
Sbjct: 360 GPGHGISIGSLGKEGTKACVSNITVRDVAMHNTMTGVRIKTWQGGVGSVKGIIFSNIQLN 419

[Close]
BLAST of Carg15622 vs. TAIR 10
Match: AT1G48100.1 (Pectin lyase-like superfamily protein )

HSP 1 Score: 174.5 bits (441), Expect = 1.3e-43
Identity = 111/358 (31.01%), Postives = 156/358 (43.58%), Query Frame = 0 

Query: 60  GASLASEGGSVHLLHDM-----------QLDGKIIAPTSPSAW--GSGLLQWIEFTKLKG 119
           G  LA EGG   +   +           QLDG ++ P  P  W       QW+ F +L G
Sbjct: 112 GVVLAPEGGVFKITSTIFSGPCKPGLVFQLDGVLMPPDGPEEWPEKDNKNQWLVFYRLDG 171

Query: 120 ITVKGTGTIDGQGSVWW---------TDDSPTDDPTDENLALRFYGSDGVTVTGITIQNS 179
            T  G GT++G G  WW          D S +  P      +RF+ S+ + V G+ IQNS
Sbjct: 172 FTFSGKGTVEGNGQKWWDLPCKPHRGPDGSSSSGPCASPTMIRFFMSNNIEVKGLRIQNS 231

Query: 180 QQAHLKFDSCTSV--SSLTISSPGDSPNTDGIHLQNSQNV-----IISNSNLACDVGTP- 239
            Q H+KFD C  V  + + ISSP  SPNTDGIHL N+++V     ++SN +    +GT  
Sbjct: 232 PQFHMKFDGCQGVLINEIQISSPKLSPNTDGIHLGNTRSVGIYNSVVSNGDDCISIGTGC 291

Query: 240 ------------------------------------------------------------ 299
                                                                       
Sbjct: 292 SDVDIQGVTCGPSHGISIGSLGVHNSQACVSNITVRNTVIRDSDNGLRVKTWQGGTGSVS 351

Query: 300 ---------------VMIDQYYCDGGRCHNGSSAVAISGVNYVNIRGTY--TSTPVHLAC 309
                          +++DQYYC    C N +SAV +  V Y NI+GTY   S P+H AC
Sbjct: 352 NLLFENIQMENVLNCIIVDQYYCQSKDCRNETSAVKVFDVEYRNIKGTYDVRSPPIHFAC 411

[Close]
BLAST of Carg15622 vs. TAIR 10
Match: AT1G56710.1 (Pectin lyase-like superfamily protein )

HSP 1 Score: 174.1 bits (440), Expect = 1.8e-43
Identity = 106/324 (32.72%), Postives = 148/324 (45.68%), Query Frame = 0 

Query: 76  MQLDGKIIAPTSPSAWGSGL-LQWIEFTKLKGITVKGTGTIDGQGSVWWT---------D 135
           +Q+DG I++P  P +W S    QW+ F ++ G++++G+G I+G+G  WW          +
Sbjct: 110 LQIDGFIVSPDGPRSWPSNYQRQWMMFYRVNGLSIQGSGVINGRGQKWWNLPCKPHKGLN 169

Query: 136 DSPTDDPTDENLALRFYGSDGVTVTGITIQNSQQAHLKFDSCTS--VSSLTISSPGDSPN 195
            +    P D  +A+R + S  V + GI   NS Q H++FD+C+   V S+ I +P  SPN
Sbjct: 170 GTTQTGPCDSPVAIRLFQSSKVRIQGINFMNSAQFHVRFDNCSDVVVDSVIIKAPASSPN 229

Query: 196 TDGIHLQNSQNVIISNS---------------------NLAC------------------ 255
           TDGIH++N+ NV I NS                     N+ C                  
Sbjct: 230 TDGIHIENTHNVQIRNSMISNGDDCISIGAGCFNVDIKNVTCGPSHGISIGSLGVHNSQA 289

Query: 256 ------------------------------------------DVGTPVMIDQYYCDGGRC 303
                                                     +V  P+MIDQYYC    C
Sbjct: 290 YVSNITVTNSTIWNSDNGVRIKTWQGGSGSVSRIVFSNILMVNVRNPIMIDQYYCQTNNC 349

The following BLAST results are available for this feature:
BLAST of Carg15622 vs. NCBI nr
Match NameE-valueIdentityDescription
KAG6588952.11.1e-16176.26Polygalacturonase, partial [Cucurbita argyrosperma subsp. sororia][more]
XP_022928201.17.2e-15665.59polygalacturonase At1g48100 [Cucurbita moschata][more]
XP_023530504.12.0e-15364.73polygalacturonase At1g48100 [Cucurbita pepo subsp. pepo][more]
XP_022989159.11.7e-15264.09polygalacturonase At1g48100 [Cucurbita maxima][more]
KAG7017769.14.5e-13459.02Polygalacturonase, partial [Cucurbita argyrosperma subsp. argyrosperma][more]
BLAST of Carg15622 vs. ExPASy Swiss-Prot
Match NameE-valueIdentityDescription
Q949Z11.9e-4231.01Polygalacturonase At1g48100 OS=Arabidopsis thaliana OX=3702 GN=At1g48100 PE=2 SV... [more]
Q94AJ51.4e-2928.16Probable polygalacturonase At1g80170 OS=Arabidopsis thaliana OX=3702 GN=At1g8017... [more]
P353364.4e-2328.01Polygalacturonase OS=Actinidia deliciosa OX=3627 PE=2 SV=1[more]
P432121.7e-2228.72Polygalacturonase OS=Cryptomeria japonica OX=3369 PE=1 SV=1[more]
O231473.1e-2125.68Polygalacturonase ADPG1 OS=Arabidopsis thaliana OX=3702 GN=ADPG1 PE=2 SV=1[more]
BLAST of Carg15622 vs. ExPASy TrEMBL
Match NameE-valueIdentityDescription
A0A6J1EJN23.5e-15665.59polygalacturonase At1g48100 OS=Cucurbita moschata OX=3662 GN=LOC111435099 PE=3 S... [more]
A0A6J1JNK88.0e-15364.09polygalacturonase At1g48100 OS=Cucurbita maxima OX=3661 GN=LOC111486313 PE=3 SV=... [more]
A0A6J1F9Z91.3e-13157.20polygalacturonase At1g48100-like OS=Cucurbita moschata OX=3662 GN=LOC111442159 P... [more]
A0A6J1J1T61.1e-13056.79polygalacturonase At1g48100-like OS=Cucurbita maxima OX=3661 GN=LOC111481937 PE=... [more]
A0A1S3B8B84.7e-12154.81polygalacturonase At1g48100 OS=Cucumis melo OX=3656 GN=LOC103487102 PE=3 SV=1[more]
BLAST of Carg15622 vs. TAIR 10
Match NameE-valueIdentityDescription
AT5G14650.12.6e-7949.52Pectin lyase-like superfamily protein [more]
AT1G60590.13.1e-6439.14Pectin lyase-like superfamily protein [more]
AT1G10640.11.6e-6038.81Pectin lyase-like superfamily protein [more]
AT1G48100.11.3e-4331.01Pectin lyase-like superfamily protein [more]
AT1G56710.11.8e-4332.72Pectin lyase-like superfamily protein [more]
InterPro
Analysis Name: InterPro Annotations of Silver-seed gourd (SMH-JMG-627) v2
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR000743Glycoside hydrolase, family 28PFAMPF00295Glyco_hydro_28coord: 205..274
e-value: 1.2E-12
score: 47.5
coord: 74..202
e-value: 9.4E-24
score: 84.1
IPR012334Pectin lyase foldGENE3D2.160.20.10coord: 205..306
e-value: 8.2E-18
score: 66.5
coord: 58..204
e-value: 5.1E-32
score: 113.3
NoneNo IPR availablePANTHERPTHR31375:SF171PECTIN LYASE-LIKE SUPERFAMILY PROTEINcoord: 205..302
coord: 76..204
NoneNo IPR availablePANTHERPTHR31375FAMILY NOT NAMEDcoord: 205..302
coord: 76..204
IPR011050Pectin lyase fold/virulence factorSUPERFAMILY51126Pectin lyase-likecoord: 75..300

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Carg15622-RACarg15622-RAmRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0005975 carbohydrate metabolic process
molecular_function GO:0004650 polygalacturonase activity