Carg15572 (gene) Silver-seed gourd (SMH-JMG-627) v2

Overview
NameCarg15572
Typegene
OrganismCucurbita argyrosperma subsp. argyrosperma cv. SMH-JMG-627 (Silver-seed gourd (SMH-JMG-627) v2)
DescriptionEF hand calcium-binding protein family
LocationCarg_Chr11: 10889368 .. 10889793 (-)
RNA-Seq ExpressionCarg15572
SyntenyCarg15572
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGATAAGTTATCGTGTTACAAGAGAGTGTTCGATCATTTTGACATGGATGGAGATGGGAAGATTTCTCCGGTCGAGGTACGGGAGTGTATAGCAGCGATATCAGGGGCGGAGGTGTCGATGGTGGAGGCAGAGGAGGTGGTGGCCGCATTCGACGTGGATGGAGACGGTGGACTTGACGTGGAGGAGTTTGAGAGGTTCGTCGACGACGGAGAGGAGGAGGAGAAGGTGAAAGAGCTTAGAGAAGCTTTTAAAATGTATGAAATGGAGGGGTCGGGATTGATTACGGCGGTGAGCTTGAGGAGGACGTTGAAGAAATTAGGAGAGTTCGTAAGTTTGGAGGATTGTATGGCTATGATTGCCAAGTTTGACCTTGACGGTGACGGTGTTCTTAGTTTTGATGAATTTAAGGTTATGATGGGATGA

mRNA sequence

ATGGATAAGTTATCGTGTTACAAGAGAGTGTTCGATCATTTTGACATGGATGGAGATGGGAAGATTTCTCCGGTCGAGGTACGGGAGTGTATAGCAGCGATATCAGGGGCGGAGGTGTCGATGGTGGAGGCAGAGGAGGTGGTGGCCGCATTCGACGTGGATGGAGACGGTGGACTTGACGTGGAGGAGTTTGAGAGGTTCGTCGACGACGGAGAGGAGGAGGAGAAGGTGAAAGAGCTTAGAGAAGCTTTTAAAATGTATGAAATGGAGGGGTCGGGATTGATTACGGCGGTGAGCTTGAGGAGGACGTTGAAGAAATTAGGAGAGTTCGTAAGTTTGGAGGATTGTATGGCTATGATTGCCAAGTTTGACCTTGACGGTGACGGTGTTCTTAGTTTTGATGAATTTAAGGTTATGATGGGATGA

Coding sequence (CDS)

ATGGATAAGTTATCGTGTTACAAGAGAGTGTTCGATCATTTTGACATGGATGGAGATGGGAAGATTTCTCCGGTCGAGGTACGGGAGTGTATAGCAGCGATATCAGGGGCGGAGGTGTCGATGGTGGAGGCAGAGGAGGTGGTGGCCGCATTCGACGTGGATGGAGACGGTGGACTTGACGTGGAGGAGTTTGAGAGGTTCGTCGACGACGGAGAGGAGGAGGAGAAGGTGAAAGAGCTTAGAGAAGCTTTTAAAATGTATGAAATGGAGGGGTCGGGATTGATTACGGCGGTGAGCTTGAGGAGGACGTTGAAGAAATTAGGAGAGTTCGTAAGTTTGGAGGATTGTATGGCTATGATTGCCAAGTTTGACCTTGACGGTGACGGTGTTCTTAGTTTTGATGAATTTAAGGTTATGATGGGATGA

Protein sequence

MDKLSCYKRVFDHFDMDGDGKISPVEVRECIAAISGAEVSMVEAEEVVAAFDVDGDGGLDVEEFERFVDDGEEEEKVKELREAFKMYEMEGSGLITAVSLRRTLKKLGEFVSLEDCMAMIAKFDLDGDGVLSFDEFKVMMG
Homology
BLAST of Carg15572 vs. NCBI nr
Match: KAG7022675.1 (putative calcium-binding protein CML19, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 273.5 bits (698), Expect = 1.0e-69
Identity = 141/141 (100.00%), Postives = 141/141 (100.00%), Query Frame = 0

Query: 1   MDKLSCYKRVFDHFDMDGDGKISPVEVRECIAAISGAEVSMVEAEEVVAAFDVDGDGGLD 60
           MDKLSCYKRVFDHFDMDGDGKISPVEVRECIAAISGAEVSMVEAEEVVAAFDVDGDGGLD
Sbjct: 1   MDKLSCYKRVFDHFDMDGDGKISPVEVRECIAAISGAEVSMVEAEEVVAAFDVDGDGGLD 60

Query: 61  VEEFERFVDDGEEEEKVKELREAFKMYEMEGSGLITAVSLRRTLKKLGEFVSLEDCMAMI 120
           VEEFERFVDDGEEEEKVKELREAFKMYEMEGSGLITAVSLRRTLKKLGEFVSLEDCMAMI
Sbjct: 61  VEEFERFVDDGEEEEKVKELREAFKMYEMEGSGLITAVSLRRTLKKLGEFVSLEDCMAMI 120

Query: 121 AKFDLDGDGVLSFDEFKVMMG 142
           AKFDLDGDGVLSFDEFKVMMG
Sbjct: 121 AKFDLDGDGVLSFDEFKVMMG 141

BLAST of Carg15572 vs. NCBI nr
Match: XP_022928328.1 (putative calcium-binding protein CML19 [Cucurbita moschata])

HSP 1 Score: 265.8 bits (678), Expect = 2.2e-67
Identity = 137/141 (97.16%), Postives = 138/141 (97.87%), Query Frame = 0

Query: 1   MDKLSCYKRVFDHFDMDGDGKISPVEVRECIAAISGAEVSMVEAEEVVAAFDVDGDGGLD 60
           MDKLSCYKRVFDHFD DGDGKISP EVRECIAAISGAEVSMVEAEEVVAAFDVDGDGGLD
Sbjct: 1   MDKLSCYKRVFDHFDTDGDGKISPFEVRECIAAISGAEVSMVEAEEVVAAFDVDGDGGLD 60

Query: 61  VEEFERFVDDGEEEEKVKELREAFKMYEMEGSGLITAVSLRRTLKKLGEFVSLEDCMAMI 120
           VEEFERFVDDGEEEEKVKELREAFKMYEM+GSGLITAVSLRRTLKKLGEFVSLEDCMAMI
Sbjct: 61  VEEFERFVDDGEEEEKVKELREAFKMYEMDGSGLITAVSLRRTLKKLGEFVSLEDCMAMI 120

Query: 121 AKFDLDGDGVLSFDEFKVMMG 142
           AKFDLD DGVLSFDEFKVMMG
Sbjct: 121 AKFDLDSDGVLSFDEFKVMMG 141

BLAST of Carg15572 vs. NCBI nr
Match: KAG6588910.1 (putative calcium-binding protein CML19, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 263.1 bits (671), Expect = 1.4e-66
Identity = 136/141 (96.45%), Postives = 137/141 (97.16%), Query Frame = 0

Query: 1   MDKLSCYKRVFDHFDMDGDGKISPVEVRECIAAISGAEVSMVEAEEVVAAFDVDGDGGLD 60
           MDKLSCYKRVFDHFD +GDGKISP EVRECIAAISGAEVSMVEAEEVVA FDVDGDGGLD
Sbjct: 1   MDKLSCYKRVFDHFDTNGDGKISPFEVRECIAAISGAEVSMVEAEEVVALFDVDGDGGLD 60

Query: 61  VEEFERFVDDGEEEEKVKELREAFKMYEMEGSGLITAVSLRRTLKKLGEFVSLEDCMAMI 120
           VEEFERFVDDGEEEEKVKELREAFKMYEMEGSGLITAVSLRRTLKKLGEFVSLEDCMAMI
Sbjct: 61  VEEFERFVDDGEEEEKVKELREAFKMYEMEGSGLITAVSLRRTLKKLGEFVSLEDCMAMI 120

Query: 121 AKFDLDGDGVLSFDEFKVMMG 142
           AKFDLD DGVLSFDEFKVMMG
Sbjct: 121 AKFDLDSDGVLSFDEFKVMMG 141

BLAST of Carg15572 vs. NCBI nr
Match: XP_023530209.1 (putative calcium-binding protein CML19 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 260.8 bits (665), Expect = 7.0e-66
Identity = 134/141 (95.04%), Postives = 137/141 (97.16%), Query Frame = 0

Query: 1   MDKLSCYKRVFDHFDMDGDGKISPVEVRECIAAISGAEVSMVEAEEVVAAFDVDGDGGLD 60
           MDKLSCYKRVFDHFDMDGDGKISP EVRECIAA+SGAEVS+VEAEEVV AFD+DGDGGLD
Sbjct: 1   MDKLSCYKRVFDHFDMDGDGKISPPEVRECIAAVSGAEVSVVEAEEVVVAFDMDGDGGLD 60

Query: 61  VEEFERFVDDGEEEEKVKELREAFKMYEMEGSGLITAVSLRRTLKKLGEFVSLEDCMAMI 120
           VEEFERFVDD  EEEKVKELREAFKMYEMEGSGLITAVSLRRTLKKLGEFVSLEDCMAMI
Sbjct: 61  VEEFERFVDDRGEEEKVKELREAFKMYEMEGSGLITAVSLRRTLKKLGEFVSLEDCMAMI 120

Query: 121 AKFDLDGDGVLSFDEFKVMMG 142
           AKFDLDGDGVLSFDEFKVMMG
Sbjct: 121 AKFDLDGDGVLSFDEFKVMMG 141

BLAST of Carg15572 vs. NCBI nr
Match: XP_022989733.1 (putative calcium-binding protein CML19 [Cucurbita maxima])

HSP 1 Score: 255.4 bits (651), Expect = 2.9e-64
Identity = 132/141 (93.62%), Postives = 135/141 (95.74%), Query Frame = 0

Query: 1   MDKLSCYKRVFDHFDMDGDGKISPVEVRECIAAISGAEVSMVEAEEVVAAFDVDGDGGLD 60
           MDKLSCYKRVFDHFDMD DGKISP EVRECIAA+SG EVSMVEAEEVV AFD+DGDGGLD
Sbjct: 1   MDKLSCYKRVFDHFDMDRDGKISPFEVRECIAAVSGEEVSMVEAEEVVGAFDMDGDGGLD 60

Query: 61  VEEFERFVDDGEEEEKVKELREAFKMYEMEGSGLITAVSLRRTLKKLGEFVSLEDCMAMI 120
           VEEFERFVDD  EEEKVKELR+AFKMYEMEGSGLITAVSLRRTLKKLGEFVSLEDCMAMI
Sbjct: 61  VEEFERFVDDIGEEEKVKELRKAFKMYEMEGSGLITAVSLRRTLKKLGEFVSLEDCMAMI 120

Query: 121 AKFDLDGDGVLSFDEFKVMMG 142
           AKFDLDGDGVLSFDEFKVMMG
Sbjct: 121 AKFDLDGDGVLSFDEFKVMMG 141

BLAST of Carg15572 vs. ExPASy Swiss-Prot
Match: Q8RYJ8 (Putative calcium-binding protein CML19 OS=Oryza sativa subsp. japonica OX=39947 GN=CML19 PE=3 SV=1)

HSP 1 Score: 138.7 bits (348), Expect = 5.2e-32
Identity = 73/137 (53.28%), Postives = 96/137 (70.07%), Query Frame = 0

Query: 7   YKRVFDHFDMDGDGKISPVEVRECIAAISGAEVSMVEAEEVVAAFDVDGDGGLDVEEFER 66
           ++RVF  FD D DGKIS  E+R C+ A  G ++S  EAE +V++ D D DG LD EEF +
Sbjct: 8   FRRVFSAFDRDADGKISAAELRLCMKAALGEDMSAEEAEALVSSADTDDDGLLDEEEFTK 67

Query: 67  F---VDDGEEEEKVKELREAFKMYEMEGSGLITAVSLRRTLKKLGEFVSLEDCMAMIAKF 126
               ++ G+EEE+ + L EAF+MYEMEG G IT  SL+R L KLG    +E+C  MI +F
Sbjct: 68  LAVQLEMGDEEERCRGLMEAFRMYEMEGEGRITPASLKRMLSKLGSHQGIEECQTMICRF 127

Query: 127 DLDGDGVLSFDEFKVMM 141
           DLDGDGV+SF+EFK+MM
Sbjct: 128 DLDGDGVISFEEFKIMM 144

BLAST of Carg15572 vs. ExPASy Swiss-Prot
Match: Q8RYJ9 (Putative calcium-binding protein CML23 OS=Oryza sativa subsp. japonica OX=39947 GN=CML23 PE=3 SV=1)

HSP 1 Score: 130.2 bits (326), Expect = 1.9e-29
Identity = 74/143 (51.75%), Postives = 94/143 (65.73%), Query Frame = 0

Query: 7   YKRVFDHFDMDGDGKISPVEVRECIAAISGAEVSMVEAEEVVAAFDVDGDGGLDVEEFER 66
           ++RVF  FD DGDGKIS  E+R C+ A  G ++   E + ++A  D DGDG LD EEF R
Sbjct: 7   FRRVFGSFDQDGDGKISATELRLCVKASLGEDMPDEEVQALMALADTDGDGLLDEEEFVR 66

Query: 67  FVD----DGEEEEKVKE-----LREAFKMYEMEGSGLITAVSLRRTLKKLGEFVSLEDCM 126
            V     DG+EEE   +     LREAF MYEMEG G IT +SL+  L KLG  + + +C 
Sbjct: 67  LVTEMEADGDEEEDDDDETCRCLREAFAMYEMEGRGCITPLSLKLMLSKLGTHLDVAECQ 126

Query: 127 AMIAKFDLDGDGVLSFDEFKVMM 141
           AMI +FD++GDGVL+FDEFK MM
Sbjct: 127 AMICRFDMNGDGVLTFDEFKTMM 149

BLAST of Carg15572 vs. ExPASy Swiss-Prot
Match: Q8RYK0 (Probable calcium-binding protein CML31 OS=Oryza sativa subsp. japonica OX=39947 GN=CML31 PE=2 SV=1)

HSP 1 Score: 130.2 bits (326), Expect = 1.9e-29
Identity = 75/135 (55.56%), Postives = 91/135 (67.41%), Query Frame = 0

Query: 10  VFDHFDMDGDGKISPVEVRECIAAISGAEVSMVEAEEVVAAFDVDGDGGLDVEEFERFVD 69
           +F  FD DGDG+IS  E+R C+    G EVS  EA ++VA+ D DGDG L   EF R V 
Sbjct: 15  LFATFDHDGDGRISAAELRLCMKTTLGEEVSDEEAGQLVASVDADGDGLLCEAEFVRLVQ 74

Query: 70  DGEEEEKVKE----LREAFKMYEMEGSGLITAVSLRRTLKKLGEFVSLEDCMAMIAKFDL 129
             E EE+ +     LREAF MYEMEG G IT  SLRR L++LG    ++DC AMI +FDL
Sbjct: 75  AAEVEEEDERRGTGLREAFGMYEMEGEGCITPTSLRRMLRRLGSDQDIDDCRAMICRFDL 134

Query: 130 DGDGVLSFDEFKVMM 141
           +GDGVLSFDEFK+MM
Sbjct: 135 NGDGVLSFDEFKIMM 149

BLAST of Carg15572 vs. ExPASy Swiss-Prot
Match: Q9FIH9 (Calcium-binding protein CML37 OS=Arabidopsis thaliana OX=3702 GN=CML37 PE=1 SV=1)

HSP 1 Score: 127.9 bits (320), Expect = 9.2e-29
Identity = 72/135 (53.33%), Postives = 94/135 (69.63%), Query Frame = 0

Query: 8   KRVFDHFDMDGDGKISPVEVRECIAAISGAEVSMVEAEEVVAAFDVDGDGGLDVEEFERF 67
           + VFD+ D + DGKIS  E++ C++ + GA +S  E EEVV   DVDGDG +D EEF + 
Sbjct: 51  RTVFDYMDANSDGKISGEELQSCVSLLGGA-LSSREVEEVVKTSDVDGDGFIDFEEFLKL 110

Query: 68  V--DDGEEEEKVKELREAFKMYEMEGSGLITAVSLRRTLKKLGEFVSLEDCMAMIAKFDL 127
           +  +DG +EE+ KEL+EAF MY MEG   ITA SLRRTL +LGE  +++ C  MI  FD 
Sbjct: 111 MEGEDGSDEERRKELKEAFGMYVMEGEEFITAASLRRTLSRLGESCTVDACKVMIRGFDQ 170

Query: 128 DGDGVLSFDEFKVMM 141
           + DGVLSFDEF +MM
Sbjct: 171 NDDGVLSFDEFVLMM 184

BLAST of Carg15572 vs. ExPASy Swiss-Prot
Match: Q2QYW1 (Probable calcium-binding protein CML25/26 OS=Oryza sativa subsp. japonica OX=39947 GN=CML25 PE=3 SV=1)

HSP 1 Score: 117.5 bits (293), Expect = 1.2e-25
Identity = 73/139 (52.52%), Postives = 92/139 (66.19%), Query Frame = 0

Query: 10  VFDHFDMDGDGKISPVEVRECIAAISGAEVSMVEAEEVVAAFDVDGDGGLDVEEFERFVD 69
           VF  FD DGDGK+S  E+R C+AA  G EVS  EA  ++A  D DGDG LD  EF R   
Sbjct: 8   VFAAFDKDGDGKVSASELRGCMAAALGEEVSEEEAAAILATADTDGDGLLDHHEFMRLSA 67

Query: 70  DGEEEEKVKE----LREAFKMY-EMEGSGLITAVSLRRTLKKLG---EFVSLEDCMAMIA 129
             + +E  +E    LREAF MY E E + +IT  SLRR L++LG   + + +E+C AMI 
Sbjct: 68  AHQLQEPAEESLRCLREAFDMYAEEEETAVITPASLRRMLRRLGSEHQRLEMEECRAMIC 127

Query: 130 KFDLDGDGVLSFDEFKVMM 141
           +FDL+GDGVLSFDEF+VMM
Sbjct: 128 RFDLNGDGVLSFDEFRVMM 146

BLAST of Carg15572 vs. ExPASy TrEMBL
Match: A0A6J1ENP8 (putative calcium-binding protein CML19 OS=Cucurbita moschata OX=3662 GN=LOC111435189 PE=4 SV=1)

HSP 1 Score: 265.8 bits (678), Expect = 1.0e-67
Identity = 137/141 (97.16%), Postives = 138/141 (97.87%), Query Frame = 0

Query: 1   MDKLSCYKRVFDHFDMDGDGKISPVEVRECIAAISGAEVSMVEAEEVVAAFDVDGDGGLD 60
           MDKLSCYKRVFDHFD DGDGKISP EVRECIAAISGAEVSMVEAEEVVAAFDVDGDGGLD
Sbjct: 1   MDKLSCYKRVFDHFDTDGDGKISPFEVRECIAAISGAEVSMVEAEEVVAAFDVDGDGGLD 60

Query: 61  VEEFERFVDDGEEEEKVKELREAFKMYEMEGSGLITAVSLRRTLKKLGEFVSLEDCMAMI 120
           VEEFERFVDDGEEEEKVKELREAFKMYEM+GSGLITAVSLRRTLKKLGEFVSLEDCMAMI
Sbjct: 61  VEEFERFVDDGEEEEKVKELREAFKMYEMDGSGLITAVSLRRTLKKLGEFVSLEDCMAMI 120

Query: 121 AKFDLDGDGVLSFDEFKVMMG 142
           AKFDLD DGVLSFDEFKVMMG
Sbjct: 121 AKFDLDSDGVLSFDEFKVMMG 141

BLAST of Carg15572 vs. ExPASy TrEMBL
Match: A0A6J1JR73 (putative calcium-binding protein CML19 OS=Cucurbita maxima OX=3661 GN=LOC111486733 PE=4 SV=1)

HSP 1 Score: 255.4 bits (651), Expect = 1.4e-64
Identity = 132/141 (93.62%), Postives = 135/141 (95.74%), Query Frame = 0

Query: 1   MDKLSCYKRVFDHFDMDGDGKISPVEVRECIAAISGAEVSMVEAEEVVAAFDVDGDGGLD 60
           MDKLSCYKRVFDHFDMD DGKISP EVRECIAA+SG EVSMVEAEEVV AFD+DGDGGLD
Sbjct: 1   MDKLSCYKRVFDHFDMDRDGKISPFEVRECIAAVSGEEVSMVEAEEVVGAFDMDGDGGLD 60

Query: 61  VEEFERFVDDGEEEEKVKELREAFKMYEMEGSGLITAVSLRRTLKKLGEFVSLEDCMAMI 120
           VEEFERFVDD  EEEKVKELR+AFKMYEMEGSGLITAVSLRRTLKKLGEFVSLEDCMAMI
Sbjct: 61  VEEFERFVDDIGEEEKVKELRKAFKMYEMEGSGLITAVSLRRTLKKLGEFVSLEDCMAMI 120

Query: 121 AKFDLDGDGVLSFDEFKVMMG 142
           AKFDLDGDGVLSFDEFKVMMG
Sbjct: 121 AKFDLDGDGVLSFDEFKVMMG 141

BLAST of Carg15572 vs. ExPASy TrEMBL
Match: A0A5D3BZX1 (Putative calcium-binding protein CML19 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold108G00900 PE=4 SV=1)

HSP 1 Score: 210.3 bits (534), Expect = 5.2e-51
Identity = 107/140 (76.43%), Postives = 122/140 (87.14%), Query Frame = 0

Query: 1   MDKLSCYKRVFDHFDMDGDGKISPVEVRECIAAISGAEVSMVEAEEVVAAFDVDGDGGLD 60
           M+KL CYKRVF+HFD DGDGKISP E+RECIA +SG ++S+VEA+E VA FD DGDG L+
Sbjct: 1   MEKLWCYKRVFEHFDSDGDGKISPSELRECIAVVSGEKLSVVEAQEAVAEFDSDGDGQLE 60

Query: 61  VEEFERFVDDGEEEEKVKELREAFKMYEMEGSGLITAVSLRRTLKKLGEFVSLEDCMAMI 120
            E+F RFVD G +EEKVKELREAFKMYEMEGSGLITA SLRR L+K+GE  SL DCMAMI
Sbjct: 61  EEDFMRFVDGGGKEEKVKELREAFKMYEMEGSGLITAESLRRMLRKMGETRSLRDCMAMI 120

Query: 121 AKFDLDGDGVLSFDEFKVMM 141
           AKFD+DGDGVL+FDEFKVMM
Sbjct: 121 AKFDVDGDGVLNFDEFKVMM 140

BLAST of Carg15572 vs. ExPASy TrEMBL
Match: A0A1S3BNF9 (putative calcium-binding protein CML19 OS=Cucumis melo OX=3656 GN=LOC103491776 PE=4 SV=1)

HSP 1 Score: 210.3 bits (534), Expect = 5.2e-51
Identity = 107/140 (76.43%), Postives = 122/140 (87.14%), Query Frame = 0

Query: 1   MDKLSCYKRVFDHFDMDGDGKISPVEVRECIAAISGAEVSMVEAEEVVAAFDVDGDGGLD 60
           M+KL CYKRVF+HFD DGDGKISP E+RECIA +SG ++S+VEA+E VA FD DGDG L+
Sbjct: 1   MEKLWCYKRVFEHFDSDGDGKISPSELRECIAVVSGEKLSVVEAQEAVAEFDSDGDGQLE 60

Query: 61  VEEFERFVDDGEEEEKVKELREAFKMYEMEGSGLITAVSLRRTLKKLGEFVSLEDCMAMI 120
            E+F RFVD G +EEKVKELREAFKMYEMEGSGLITA SLRR L+K+GE  SL DCMAMI
Sbjct: 61  EEDFMRFVDGGGKEEKVKELREAFKMYEMEGSGLITAESLRRMLRKMGETRSLRDCMAMI 120

Query: 121 AKFDLDGDGVLSFDEFKVMM 141
           AKFD+DGDGVL+FDEFKVMM
Sbjct: 121 AKFDVDGDGVLNFDEFKVMM 140

BLAST of Carg15572 vs. ExPASy TrEMBL
Match: A0A0A0L8P1 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_3G588520 PE=4 SV=1)

HSP 1 Score: 204.1 bits (518), Expect = 3.7e-49
Identity = 104/140 (74.29%), Postives = 119/140 (85.00%), Query Frame = 0

Query: 1   MDKLSCYKRVFDHFDMDGDGKISPVEVRECIAAISGAEVSMVEAEEVVAAFDVDGDGGLD 60
           M+K  CYKRVF+HFD DGDGKISP E+RECIA +SG ++S++EA+E VA FD DGDG L 
Sbjct: 1   MEKPRCYKRVFEHFDSDGDGKISPSELRECIAVVSGEKLSVLEAQEAVAEFDSDGDGQLK 60

Query: 61  VEEFERFVDDGEEEEKVKELREAFKMYEMEGSGLITAVSLRRTLKKLGEFVSLEDCMAMI 120
            E+F RFVD G EEE+VKELREAFKMYEM+GSGLITA SLRR L+KLGE  SL DC AMI
Sbjct: 61  EEDFVRFVDGGGEEERVKELREAFKMYEMKGSGLITAESLRRMLRKLGETKSLRDCTAMI 120

Query: 121 AKFDLDGDGVLSFDEFKVMM 141
           AKFD+DGDGVLSFDEF+VMM
Sbjct: 121 AKFDIDGDGVLSFDEFRVMM 140

BLAST of Carg15572 vs. TAIR 10
Match: AT5G42380.1 (calmodulin like 37 )

HSP 1 Score: 127.9 bits (320), Expect = 6.6e-30
Identity = 72/135 (53.33%), Postives = 94/135 (69.63%), Query Frame = 0

Query: 8   KRVFDHFDMDGDGKISPVEVRECIAAISGAEVSMVEAEEVVAAFDVDGDGGLDVEEFERF 67
           + VFD+ D + DGKIS  E++ C++ + GA +S  E EEVV   DVDGDG +D EEF + 
Sbjct: 51  RTVFDYMDANSDGKISGEELQSCVSLLGGA-LSSREVEEVVKTSDVDGDGFIDFEEFLKL 110

Query: 68  V--DDGEEEEKVKELREAFKMYEMEGSGLITAVSLRRTLKKLGEFVSLEDCMAMIAKFDL 127
           +  +DG +EE+ KEL+EAF MY MEG   ITA SLRRTL +LGE  +++ C  MI  FD 
Sbjct: 111 MEGEDGSDEERRKELKEAFGMYVMEGEEFITAASLRRTLSRLGESCTVDACKVMIRGFDQ 170

Query: 128 DGDGVLSFDEFKVMM 141
           + DGVLSFDEF +MM
Sbjct: 171 NDDGVLSFDEFVLMM 184

BLAST of Carg15572 vs. TAIR 10
Match: AT1G76650.1 (calmodulin-like 38 )

HSP 1 Score: 110.5 bits (275), Expect = 1.1e-24
Identity = 65/139 (46.76%), Postives = 87/139 (62.59%), Query Frame = 0

Query: 2   DKLSCYKRVFDHFDMDGDGKISPVEVRECIAAISGAEVSMVEAEEVVAAFDVDGDGGLDV 61
           DK    + VF + D + DG+ISP E+++    + G ++S  EA   V   D DGDG LD 
Sbjct: 39  DKNRELEAVFSYMDANRDGRISPEELQKSFMTL-GEQLSDEEAVAAVRLSDTDGDGMLDF 98

Query: 62  EEFERFVDDGEEEEKVKELREAFKMYEMEGSGLITAVSLRRTLKKLGEFVSLEDCMAMIA 121
           EEF + +   +EEEK  EL+ AF++Y  EG   IT  SL+  LKKLGE  + +DC  MI+
Sbjct: 99  EEFSQLIKVDDEEEKKMELKGAFRLYIAEGEDCITPRSLKMMLKKLGESRTTDDCRVMIS 158

Query: 122 KFDLDGDGVLSFDEFKVMM 141
            FDL+ DGVLSFDEF +MM
Sbjct: 159 AFDLNADGVLSFDEFALMM 176

BLAST of Carg15572 vs. TAIR 10
Match: AT1G76650.3 (calmodulin-like 38 )

HSP 1 Score: 110.5 bits (275), Expect = 1.1e-24
Identity = 65/139 (46.76%), Postives = 87/139 (62.59%), Query Frame = 0

Query: 2   DKLSCYKRVFDHFDMDGDGKISPVEVRECIAAISGAEVSMVEAEEVVAAFDVDGDGGLDV 61
           DK    + VF + D + DG+ISP E+++    + G ++S  EA   V   D DGDG LD 
Sbjct: 39  DKNRELEAVFSYMDANRDGRISPEELQKSFMTL-GEQLSDEEAVAAVRLSDTDGDGMLDF 98

Query: 62  EEFERFVDDGEEEEKVKELREAFKMYEMEGSGLITAVSLRRTLKKLGEFVSLEDCMAMIA 121
           EEF + +   +EEEK  EL+ AF++Y  EG   IT  SL+  LKKLGE  + +DC  MI+
Sbjct: 99  EEFSQLIKVDDEEEKKMELKGAFRLYIAEGEDCITPRSLKMMLKKLGESRTTDDCRVMIS 158

Query: 122 KFDLDGDGVLSFDEFKVMM 141
            FDL+ DGVLSFDEF +MM
Sbjct: 159 AFDLNADGVLSFDEFALMM 176

BLAST of Carg15572 vs. TAIR 10
Match: AT5G37770.1 (EF hand calcium-binding protein family )

HSP 1 Score: 107.5 bits (267), Expect = 9.2e-24
Identity = 60/138 (43.48%), Postives = 88/138 (63.77%), Query Frame = 0

Query: 8   KRVFDHFDMDGDGKISPVEVRECIAAISGAEVSMVEAEEVVAAFDVDGDGGLDVEEFERF 67
           K+VF  FD +GDGKIS  E++E I A+S    S  E   ++  FD+DG+G +D++EF   
Sbjct: 19  KKVFQRFDKNGDGKISVDELKEVIRALS-PTASPEETVTMMKQFDLDGNGFIDLDEFVAL 78

Query: 68  VD-----DGEEEEKVKELREAFKMYEMEGSGLITAVSLRRTLKKLGEFVSLEDCMAMIAK 127
                   G     V +L+EAF++Y+++G+G I+A  L   +K LGE  S++DC  MI+K
Sbjct: 79  FQIGIGGGGNNRNDVSDLKEAFELYDLDGNGRISAKELHSVMKNLGEKCSVQDCKKMISK 138

Query: 128 FDLDGDGVLSFDEFKVMM 141
            D+DGDG ++FDEFK MM
Sbjct: 139 VDIDGDGCVNFDEFKKMM 155

BLAST of Carg15572 vs. TAIR 10
Match: AT3G01830.1 (Calcium-binding EF-hand family protein )

HSP 1 Score: 102.8 bits (255), Expect = 2.3e-22
Identity = 59/142 (41.55%), Postives = 91/142 (64.08%), Query Frame = 0

Query: 1   MDKLSCYKRVFDHFDMDGDGKISPVEVRECIAAISGAEVSMVEAEEVVAAFDVDG--DGG 60
           ++K   Y+RVF  FD    GK+S   +  C+ AI   + ++V+ E+       +   D  
Sbjct: 6   VNKRDEYQRVFSCFDKSHQGKVSVSTIERCVDAIKSGKRAVVDQEDTTNPNPEESTDDKS 65

Query: 61  LDVEEFERFVDDGEEEEKVKELREAFKMYEMEGSGLITAVSLRRTLKKLGEFVSLEDCMA 120
           L++E+F + V++GEE +K K+L+EAFK+YE E  G IT  SL+R L  LGE  SL+DC  
Sbjct: 66  LELEDFVKLVEEGEEADKEKDLKEAFKLYE-ESEG-ITPKSLKRMLSLLGESKSLKDCEV 125

Query: 121 MIAKFDLDGDGVLSFDEFKVMM 141
           MI++FD++ DG+++FDEF+ MM
Sbjct: 126 MISQFDINRDGIINFDEFRAMM 145

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
KAG7022675.11.0e-69100.00putative calcium-binding protein CML19, partial [Cucurbita argyrosperma subsp. a... [more]
XP_022928328.12.2e-6797.16putative calcium-binding protein CML19 [Cucurbita moschata][more]
KAG6588910.11.4e-6696.45putative calcium-binding protein CML19, partial [Cucurbita argyrosperma subsp. s... [more]
XP_023530209.17.0e-6695.04putative calcium-binding protein CML19 [Cucurbita pepo subsp. pepo][more]
XP_022989733.12.9e-6493.62putative calcium-binding protein CML19 [Cucurbita maxima][more]
Match NameE-valueIdentityDescription
Q8RYJ85.2e-3253.28Putative calcium-binding protein CML19 OS=Oryza sativa subsp. japonica OX=39947 ... [more]
Q8RYJ91.9e-2951.75Putative calcium-binding protein CML23 OS=Oryza sativa subsp. japonica OX=39947 ... [more]
Q8RYK01.9e-2955.56Probable calcium-binding protein CML31 OS=Oryza sativa subsp. japonica OX=39947 ... [more]
Q9FIH99.2e-2953.33Calcium-binding protein CML37 OS=Arabidopsis thaliana OX=3702 GN=CML37 PE=1 SV=1[more]
Q2QYW11.2e-2552.52Probable calcium-binding protein CML25/26 OS=Oryza sativa subsp. japonica OX=399... [more]
Match NameE-valueIdentityDescription
A0A6J1ENP81.0e-6797.16putative calcium-binding protein CML19 OS=Cucurbita moschata OX=3662 GN=LOC11143... [more]
A0A6J1JR731.4e-6493.62putative calcium-binding protein CML19 OS=Cucurbita maxima OX=3661 GN=LOC1114867... [more]
A0A5D3BZX15.2e-5176.43Putative calcium-binding protein CML19 OS=Cucumis melo var. makuwa OX=1194695 GN... [more]
A0A1S3BNF95.2e-5176.43putative calcium-binding protein CML19 OS=Cucumis melo OX=3656 GN=LOC103491776 P... [more]
A0A0A0L8P13.7e-4974.29Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_3G588520 PE=4 SV=1[more]
Match NameE-valueIdentityDescription
AT5G42380.16.6e-3053.33calmodulin like 37 [more]
AT1G76650.11.1e-2446.76calmodulin-like 38 [more]
AT1G76650.31.1e-2446.76calmodulin-like 38 [more]
AT5G37770.19.2e-2443.48EF hand calcium-binding protein family [more]
AT3G01830.12.3e-2241.55Calcium-binding EF-hand family protein [more]
InterPro
Analysis Name: InterPro Annotations of Silver-seed gourd (SMH-JMG-627) v2
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR002048EF-hand domainSMARTSM00054efh_1coord: 6..34
e-value: 0.0076
score: 25.5
coord: 115..141
e-value: 0.77
score: 18.7
coord: 79..107
e-value: 2.3
score: 14.8
coord: 43..71
e-value: 3.2
score: 13.7
IPR002048EF-hand domainPFAMPF13499EF-hand_7coord: 8..68
e-value: 4.6E-10
score: 39.8
IPR002048EF-hand domainPFAMPF13833EF-hand_8coord: 92..140
e-value: 5.7E-11
score: 42.0
IPR002048EF-hand domainPROSITEPS50222EF_HAND_2coord: 111..141
score: 14.708673
IPR002048EF-hand domainPROSITEPS50222EF_HAND_2coord: 39..74
score: 11.277538
IPR002048EF-hand domainPROSITEPS50222EF_HAND_2coord: 2..37
score: 12.560727
IPR002048EF-hand domainPROSITEPS50222EF_HAND_2coord: 75..110
score: 9.63171
IPR002048EF-hand domainCDDcd00051EFhcoord: 79..140
e-value: 3.2116E-13
score: 58.3281
NoneNo IPR availableGENE3D1.10.238.10coord: 1..72
e-value: 1.7E-15
score: 59.2
NoneNo IPR availableGENE3D1.10.238.10coord: 73..141
e-value: 9.0E-20
score: 72.7
NoneNo IPR availablePANTHERPTHR10891EF-HAND CALCIUM-BINDING DOMAIN CONTAINING PROTEINcoord: 2..140
NoneNo IPR availablePANTHERPTHR10891:SF858EF HAND CALCIUM-BINDING FAMILY PROTEINcoord: 2..140
IPR018247EF-Hand 1, calcium-binding sitePROSITEPS00018EF_HAND_1coord: 124..136
IPR018247EF-Hand 1, calcium-binding sitePROSITEPS00018EF_HAND_1coord: 15..27
IPR011992EF-hand domain pairSUPERFAMILY47473EF-handcoord: 4..140

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Carg15572-RACarg15572-RAmRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
molecular_function GO:0005509 calcium ion binding