Carg15545 (gene) Silver-seed gourd (SMH-JMG-627) v2

Overview


Name	Carg15545
Type	gene
Organism	Cucurbita argyrosperma subsp. argyrosperma cv. SMH-JMG-627 (Silver-seed gourd (SMH-JMG-627) v2)
Description	major pollen allergen Ole e 1
Location	Carg_Chr11: 10735988 .. 10737947 (+)
RNA-Seq Expression	Carg15545
Synteny	Carg15545

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRCDSpolypeptidethree_prime_UTR

Hold the cursor over a type above to highlight its positions in the sequence below.

TGTCTTAACCTTCATCTCATTGCCTAGTTAAGATTGCTTGGCTCAATCGACATTAGCTAAGACTACAGCCCCGTAATTGTCGCCGACCAAAGCTTGAAAACTTAAAGCACTTAGATGTCTGAATGTTTGAGGGTTGAGATGTAGAGCAGGCATTCTTTGTTCACAAGAGACTTGGAATTGACACACCTAAAAACAGGTGATGGGTATGAATCTTCTCAAGACAATTTAAAGGCATTCAATCCATATGTGTTGGTTGGCTCTTTAGTTTGAAATGGGTAGCTCTATTAAGAGAGTTTAGCTGGGTTAGGCCCTCGAATCAAGAAGCAATATCAGCAATAATTCTGCCAAAAGAATCTACTTTCTTCTCTTTCCTACGCTTCAAGTTCACATGGAAACTGACAAAATATCATCACATGTCTTTGTTTCTTGTGAAAATGTTAAATGGCCTTTACTTTTTGCCTTTACTTCAGTTATATCCCTACCCACCAACTGCTGAAGCAGTGAAGCTCGTTGCTTACATTACTACTACTACTACGCCCACCCTTCATTACATATCCTGATATGCATTGAGCATCAGAGGGCACTTCTTTCAAAGAGGAAAGAAACAGAAGCTTCTCTCTTGTTCCAACAGTTAGCCATGAATCTCTTTCTACTCTTTATCTTAGCTTTCTTGGTTCCAAGTTCCCTTCTATTGGGAATTCAGGCTTCTCATTCCCACCGAACAAGAACATTTTCTCAGATTACGGTCATGGGCCTTGTTTATTGTGACACTTGCTCCAACAACTCCTTCTCCAAACACAGTTACTTCTTATCAGGTAAGGCTCATATTCATTTGAAGTCATGATTTCAACAACTTGAACATGAACTAGAATTCAAATGATGAAACTAGTATCTTGTTTTCAAGAGCAATGGTTTTTGTAGTTATGTTGTACGTTTGTTTGGACTTTAATCTTCTTCAGCATTTTGATACTGATTTTGAATTTGATTTTCAACTTCTTTTTCCTTTTCAAGAGCAATGGCTTTTGTAGTTATGTTGTACGTTTGTTTAGACTTTAATCTTCTTCAGCATTTTGATACTGATTTTGAATTTGATTTTCAACTTCGTTGATACAATTTTCTAACCTAATTTCAATAAAAGCATTTAATTCAGCAATTCATTCAAAAGCAAGACTGATAAAAATCGATACTCGTCGGTGCACTGCGTGTGTGTTTATGTATGAAAAAAATCAGGTGTAGAAGTTCGAATCAGCTGCAAATTCAAAGCAGAAACGCCGCGTACGGCGGAGCAGATAGCATTCTCAGTGAACAGAACAACAAACAAATACGGAGTTTACAGATTGGAAATACCTTCGGTGGACGGAATCCGATGCGCAGAGGATTCAGAGATAGCGTCGTTCTGCCAAGCCAGTTTGATCGGAAGTCCATCGTCATCTTGCAACGTCCCTGGATACAGAACCACATCGGAAGAAATTTCGATCAAATCGAGAGAGGCAAACATTTGCATTTACAGCTTAAATCCGCTCAATTACAGGCCATCAGCTAGAGATGATACCTTGTGTGGAAATTAGTCGAACGATGAAGATGATTACCTTTTGATGAAGCTCCCTTTTGCCTCGCCCAAATTTGTCGCCATGGATTGAACAAAATTGTACAGTTTTGGAAGGAATCAAAGCTCTCGTCTCTCTCAATCTCTTTGCAATTTAACCTTCCAAAGTTAATATTTGCATGATTTATATATTATCAACTAAAATATCAAAATTTTCTCAACTAATATTCATATTTGCAATTTAGTCCTTCCTAGTGTTAAAATTTTCACTATCGTCTCCGTTTCACCCCTCATACCTTTGCAATTTAGCCCTTCGAAACTTAATATTTACATAATTTATATACTCTCGACTAAAATGTCAAAATTTTCGCTTTCGTATAATTGAACTAACATTCATATTTGCAATTTAGTCCT

mRNA sequence

TGTCTTAACCTTCATCTCATTGCCTAGTTAAGATTGCTTGGCTCAATCGACATTAGCTAAGACTACAGCCCCGTAATTGTCGCCGACCAAAGCTTGAAAACTTAAAGCACTTAGATGTCTGAATGTTTGAGGGTTGAGATGTAGAGCAGGCATTCTTTGTTCACAAGAGACTTGGAATTGACACACCTAAAAACAGGTGATGGGTATGAATCTTCTCAAGACAATTTAAAGGCATTCAATCCATATGTGTTGGTTGGCTCTTTAGTTTGAAATGGGTAGCTCTATTAAGAGAGTTTAGCTGGGTTAGGCCCTCGAATCAAGAAGCAATATCAGCAATAATTCTGCCAAAAGAATCTACTTTCTTCTCTTTCCTACGCTTCAAGTTCACATGGAAACTGACAAAATATCATCACATGTCTTTGTTTCTTGTGAAAATGTTAAATGGCCTTTACTTTTTGCCTTTACTTCAGTTATATCCCTACCCACCAACTGCTGAAGCAGTGAAGCTCGTTGCTTACATTACTACTACTACTACGCCCACCCTTCATTACATATCCTGATATGCATTGAGCATCAGAGGGCACTTCTTTCAAAGAGGAAAGAAACAGAAGCTTCTCTCTTGTTCCAACAGTTAGCCATGAATCTCTTTCTACTCTTTATCTTAGCTTTCTTGGTTCCAAGTTCCCTTCTATTGGGAATTCAGGCTTCTCATTCCCACCGAACAAGAACATTTTCTCAGATTACGGTCATGGGCCTTGTTTATTGTGACACTTGCTCCAACAACTCCTTCTCCAAACACAGTTACTTCTTATCAGGTGTAGAAGTTCGAATCAGCTGCAAATTCAAAGCAGAAACGCCGCGTACGGCGGAGCAGATAGCATTCTCAGTGAACAGAACAACAAACAAATACGGAGTTTACAGATTGGAAATACCTTCGGTGGACGGAATCCGATGCGCAGAGGATTCAGAGATAGCGTCGTTCTGCCAAGCCAGTTTGATCGGAAGTCCATCGTCATCTTGCAACGTCCCTGGATACAGAACCACATCGGAAGAAATTTCGATCAAATCGAGAGAGGCAAACATTTGCATTTACAGCTTAAATCCGCTCAATTACAGGCCATCAGCTAGAGATGATACCTTGTGTGGAAATTAGTCGAACGATGAAGATGATTACCTTTTGATGAAGCTCCCTTTTGCCTCGCCCAAATTTGTCGCCATGGATTGAACAAAATTGTACAGTTTTGGAAGGAATCAAAGCTCTCGTCTCTCTCAATCTCTTTGCAATTTAACCTTCCAAAGTTAATATTTGCATGATTTATATATTATCAACTAAAATATCAAAATTTTCTCAACTAATATTCATATTTGCAATTTAGTCCTTCCTAGTGTTAAAATTTTCACTATCGTCTCCGTTTCACCCCTCATACCTTTGCAATTTAGCCCTTCGAAACTTAATATTTACATAATTTATATACTCTCGACTAAAATGTCAAAATTTTCGCTTTCGTATAATTGAACTAACATTCATATTTGCAATTTAGTCCT

Coding sequence (CDS)

ATGAATCTCTTTCTACTCTTTATCTTAGCTTTCTTGGTTCCAAGTTCCCTTCTATTGGGAATTCAGGCTTCTCATTCCCACCGAACAAGAACATTTTCTCAGATTACGGTCATGGGCCTTGTTTATTGTGACACTTGCTCCAACAACTCCTTCTCCAAACACAGTTACTTCTTATCAGGTGTAGAAGTTCGAATCAGCTGCAAATTCAAAGCAGAAACGCCGCGTACGGCGGAGCAGATAGCATTCTCAGTGAACAGAACAACAAACAAATACGGAGTTTACAGATTGGAAATACCTTCGGTGGACGGAATCCGATGCGCAGAGGATTCAGAGATAGCGTCGTTCTGCCAAGCCAGTTTGATCGGAAGTCCATCGTCATCTTGCAACGTCCCTGGATACAGAACCACATCGGAAGAAATTTCGATCAAATCGAGAGAGGCAAACATTTGCATTTACAGCTTAAATCCGCTCAATTACAGGCCATCAGCTAGAGATGATACCTTGTGTGGAAATTAG

Protein sequence

MNLFLLFILAFLVPSSLLLGIQASHSHRTRTFSQITVMGLVYCDTCSNNSFSKHSYFLSGVEVRISCKFKAETPRTAEQIAFSVNRTTNKYGVYRLEIPSVDGIRCAEDSEIASFCQASLIGSPSSSCNVPGYRTTSEEISIKSREANICIYSLNPLNYRPSARDDTLCGN

Homology

[Close]

BLAST of Carg15545 vs. NCBI nr
Match: XP_022928243.1 (pollen-specific protein-like At4g18596 [Cucurbita moschata] >XP_023530922.1 pollen-specific protein-like At4g18596 isoform X1 [Cucurbita pepo subsp. pepo] >XP_023530923.1 pollen-specific protein-like At4g18596 isoform X2 [Cucurbita pepo subsp. pepo] >XP_023530924.1 pollen-specific protein-like At4g18596 [Cucurbita pepo subsp. pepo] >KAG7022648.1 Pollen-specific protein-like protein [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 341.7 bits (875), Expect = 3.8e-90
Identity = 171/171 (100.00%), Postives = 171/171 (100.00%), Query Frame = 0


Query: 1   MNLFLLFILAFLVPSSLLLGIQASHSHRTRTFSQITVMGLVYCDTCSNNSFSKHSYFLSG 60
           MNLFLLFILAFLVPSSLLLGIQASHSHRTRTFSQITVMGLVYCDTCSNNSFSKHSYFLSG
Sbjct: 1   MNLFLLFILAFLVPSSLLLGIQASHSHRTRTFSQITVMGLVYCDTCSNNSFSKHSYFLSG 60

Query: 61  VEVRISCKFKAETPRTAEQIAFSVNRTTNKYGVYRLEIPSVDGIRCAEDSEIASFCQASL 120
           VEVRISCKFKAETPRTAEQIAFSVNRTTNKYGVYRLEIPSVDGIRCAEDSEIASFCQASL
Sbjct: 61  VEVRISCKFKAETPRTAEQIAFSVNRTTNKYGVYRLEIPSVDGIRCAEDSEIASFCQASL 120

Query: 121 IGSPSSSCNVPGYRTTSEEISIKSREANICIYSLNPLNYRPSARDDTLCGN 172
           IGSPSSSCNVPGYRTTSEEISIKSREANICIYSLNPLNYRPSARDDTLCGN
Sbjct: 121 IGSPSSSCNVPGYRTTSEEISIKSREANICIYSLNPLNYRPSARDDTLCGN 171

[Close]

BLAST of Carg15545 vs. NCBI nr
Match: XP_022989732.1 (pollen-specific protein-like At4g18596 [Cucurbita maxima])

HSP 1 Score: 338.2 bits (866), Expect = 4.2e-89
Identity = 169/171 (98.83%), Postives = 170/171 (99.42%), Query Frame = 0


Query: 1   MNLFLLFILAFLVPSSLLLGIQASHSHRTRTFSQITVMGLVYCDTCSNNSFSKHSYFLSG 60
           MNLFLLFILAFLVPSSLLLGIQASHSHRTRTF+QITVMGLVYCDTCSNNSFSKHSYFLSG
Sbjct: 1   MNLFLLFILAFLVPSSLLLGIQASHSHRTRTFTQITVMGLVYCDTCSNNSFSKHSYFLSG 60

Query: 61  VEVRISCKFKAETPRTAEQIAFSVNRTTNKYGVYRLEIPSVDGIRCAEDSEIASFCQASL 120
           VEVRISCKFKAETPRTAEQIAFSVNRTTNKYGVYRLEIPSVDGIRCAEDS IASFCQASL
Sbjct: 61  VEVRISCKFKAETPRTAEQIAFSVNRTTNKYGVYRLEIPSVDGIRCAEDSAIASFCQASL 120

Query: 121 IGSPSSSCNVPGYRTTSEEISIKSREANICIYSLNPLNYRPSARDDTLCGN 172
           IGSPSSSCNVPGYRTTSEEISIKSREANICIYSLNPLNYRPSARDDTLCGN
Sbjct: 121 IGSPSSSCNVPGYRTTSEEISIKSREANICIYSLNPLNYRPSARDDTLCGN 171

[Close]

BLAST of Carg15545 vs. NCBI nr
Match: KAG6588884.1 (Glutaredoxin-C1, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 306.2 bits (783), Expect = 1.8e-79
Identity = 149/149 (100.00%), Postives = 149/149 (100.00%), Query Frame = 0


Query: 23  ASHSHRTRTFSQITVMGLVYCDTCSNNSFSKHSYFLSGVEVRISCKFKAETPRTAEQIAF 82
           ASHSHRTRTFSQITVMGLVYCDTCSNNSFSKHSYFLSGVEVRISCKFKAETPRTAEQIAF
Sbjct: 228 ASHSHRTRTFSQITVMGLVYCDTCSNNSFSKHSYFLSGVEVRISCKFKAETPRTAEQIAF 287

Query: 83  SVNRTTNKYGVYRLEIPSVDGIRCAEDSEIASFCQASLIGSPSSSCNVPGYRTTSEEISI 142
           SVNRTTNKYGVYRLEIPSVDGIRCAEDSEIASFCQASLIGSPSSSCNVPGYRTTSEEISI
Sbjct: 288 SVNRTTNKYGVYRLEIPSVDGIRCAEDSEIASFCQASLIGSPSSSCNVPGYRTTSEEISI 347

Query: 143 KSREANICIYSLNPLNYRPSARDDTLCGN 172
           KSREANICIYSLNPLNYRPSARDDTLCGN
Sbjct: 348 KSREANICIYSLNPLNYRPSARDDTLCGN 376

[Close]

BLAST of Carg15545 vs. NCBI nr
Match: XP_038896993.1 (major pollen allergen Ole e 1-like [Benincasa hispida])

HSP 1 Score: 299.3 bits (765), Expect = 2.1e-77
Identity = 146/171 (85.38%), Postives = 159/171 (92.98%), Query Frame = 0


Query: 1   MNLFLLFILAFLVPSSLLLGIQASHSHRTRTFSQITVMGLVYCDTCSNNSFSKHSYFLSG 60
           MNL LLF+LA LVPSSLLL IQA+HSH +RT+S ITVMGLVYCDTCSNN+FSKHSYFLSG
Sbjct: 1   MNLILLFVLASLVPSSLLLEIQAAHSHPSRTYSHITVMGLVYCDTCSNNTFSKHSYFLSG 60

Query: 61  VEVRISCKFKAETPRTAEQIAFSVNRTTNKYGVYRLEIPSVDGIRCAEDSEIASFCQASL 120
           VEVRI CKFKAETPRTAEQIAFSVNRTTN+YG+Y+LEIPSVDGIRCAEDS + SFCQASL
Sbjct: 61  VEVRIDCKFKAETPRTAEQIAFSVNRTTNRYGIYKLEIPSVDGIRCAEDSAMVSFCQASL 120

Query: 121 IGSPSSSCNVPGYRTTSEEISIKSREANICIYSLNPLNYRPSARDDTLCGN 172
           +GS SSSCNVPGYR+TS EISIKSR++NICIYSLNPLNYRPS RDD LCGN
Sbjct: 121 MGSSSSSCNVPGYRSTSGEISIKSRQSNICIYSLNPLNYRPSTRDDILCGN 171

[Close]

BLAST of Carg15545 vs. NCBI nr
Match: XP_022156533.1 (major pollen allergen Ole e 1 [Momordica charantia])

HSP 1 Score: 292.7 bits (748), Expect = 2.0e-75
Identity = 145/171 (84.80%), Postives = 154/171 (90.06%), Query Frame = 0


Query: 1   MNLFLLFILAFLVPSSLLLGIQASHSHRTRTFSQITVMGLVYCDTCSNNSFSKHSYFLSG 60
           MNL L F+ AFLVPSSLLL  +A+ S  T T+SQITVMGLVYCDTCSNN+FSKHSYFLSG
Sbjct: 1   MNLILFFVFAFLVPSSLLLESEAARSQPTGTYSQITVMGLVYCDTCSNNTFSKHSYFLSG 60

Query: 61  VEVRISCKFKAETPRTAEQIAFSVNRTTNKYGVYRLEIPSVDGIRCAEDSEIASFCQASL 120
            EVRI CKFKAETPRTAEQIAFSVNRTTNKYGVY+LEIPSVDGIRCAEDS + SFCQASL
Sbjct: 61  AEVRIDCKFKAETPRTAEQIAFSVNRTTNKYGVYKLEIPSVDGIRCAEDSAMVSFCQASL 120

Query: 121 IGSPSSSCNVPGYRTTSEEISIKSREANICIYSLNPLNYRPSARDDTLCGN 172
           IGS SSSCNVPGYR+TS EISIKSR+ANICIYSLNPLNYRPS RDDTLC N
Sbjct: 121 IGSASSSCNVPGYRSTSGEISIKSRQANICIYSLNPLNYRPSTRDDTLCAN 171

[Close]

BLAST of Carg15545 vs. ExPASy Swiss-Prot
Match: Q6NMJ2 (Pollen-specific protein-like At4g18596 OS=Arabidopsis thaliana OX=3702 GN=At4g18596 PE=2 SV=1)

HSP 1 Score: 50.1 bits (118), Expect = 3.0e-05
Identity = 42/162 (25.93%), Postives = 73/162 (45.06%), Query Frame = 0


Query: 3   LFLLFILAFLVPSSLLLGIQASHSHRTRTFSQITVMGLVYCDTCSNNSFSKHSYFLSGVE 62
           +F  F+ A  + S  L G+  + +     F +  + G VYCDTC     ++ S FL G +
Sbjct: 6   IFFFFVSAVCLSS--LAGVAIADAD---DFDRFQIQGSVYCDTCRVQFVTRLSKFLEGAK 65

Query: 63  VRISCKFKAETPRTAEQIAFSVNRTTNKYGVYRLEIPSVDGIRCAEDSEIASFCQASLIG 122
           V++ C+      RT   +  +    T+K G YR+E+          D E    C+  L+ 
Sbjct: 66  VKLECR-----SRTNGTVTLTKEAVTDKTGSYRMEVTG--------DHE-EEVCELVLVE 125

Query: 123 SPSSSC---NVPGYRTTSEEISIKSREANICIYS--LNPLNY 160
           SP S C   +   Y   + +IS+ + +  +   +  +NPL +
Sbjct: 126 SPDSGCSDVSKEAYLRNAAKISLTANDGIVSHETRIVNPLGF 148

[Close]

BLAST of Carg15545 vs. ExPASy Swiss-Prot
Match: Q8LGR0 (Pollen allergen Che a 1 OS=Chenopodium album OX=3559 PE=1 SV=1)

HSP 1 Score: 45.1 bits (105), Expect = 9.5e-04
Identity = 35/127 (27.56%), Postives = 53/127 (41.73%), Query Frame = 0


Query: 37  VMGLVYCDTCSNNSFSKHSYFLSGVEVRISCKFKAETPRTAEQIAFSVNRTTNKYGVYRL 96
           V G+VYCDTC     ++ S  + G  V++ C+       TA    F     T+K G Y +
Sbjct: 32  VQGMVYCDTCRIQFMTRISTIMEGATVKLECR-----NITAGTQTFKAEAVTDKVGQYSI 91

Query: 97  EIPSVDGIRCAEDSEIASFCQASLIGSPSSSCNVPG---YRTTSEEISIKSREANIC-IY 156
            +          D E    C+  L+ SP+S C+      Y   S ++S+ S       I 
Sbjct: 92  PVNG--------DFE-DDICEIELVKSPNSECSEVSHDVYAKQSAKVSLTSNNGEASDIR 144

Query: 157 SLNPLNY 160
           S N L +
Sbjct: 152 SANALGF 144

[Close]

BLAST of Carg15545 vs. ExPASy TrEMBL
Match: A0A6J1ENF6 (pollen-specific protein-like At4g18596 OS=Cucurbita moschata OX=3662 GN=LOC111435137 PE=4 SV=1)

HSP 1 Score: 341.7 bits (875), Expect = 1.8e-90
Identity = 171/171 (100.00%), Postives = 171/171 (100.00%), Query Frame = 0


Query: 1   MNLFLLFILAFLVPSSLLLGIQASHSHRTRTFSQITVMGLVYCDTCSNNSFSKHSYFLSG 60
           MNLFLLFILAFLVPSSLLLGIQASHSHRTRTFSQITVMGLVYCDTCSNNSFSKHSYFLSG
Sbjct: 1   MNLFLLFILAFLVPSSLLLGIQASHSHRTRTFSQITVMGLVYCDTCSNNSFSKHSYFLSG 60

Query: 61  VEVRISCKFKAETPRTAEQIAFSVNRTTNKYGVYRLEIPSVDGIRCAEDSEIASFCQASL 120
           VEVRISCKFKAETPRTAEQIAFSVNRTTNKYGVYRLEIPSVDGIRCAEDSEIASFCQASL
Sbjct: 61  VEVRISCKFKAETPRTAEQIAFSVNRTTNKYGVYRLEIPSVDGIRCAEDSEIASFCQASL 120

Query: 121 IGSPSSSCNVPGYRTTSEEISIKSREANICIYSLNPLNYRPSARDDTLCGN 172
           IGSPSSSCNVPGYRTTSEEISIKSREANICIYSLNPLNYRPSARDDTLCGN
Sbjct: 121 IGSPSSSCNVPGYRTTSEEISIKSREANICIYSLNPLNYRPSARDDTLCGN 171

[Close]

BLAST of Carg15545 vs. ExPASy TrEMBL
Match: A0A6J1JGM9 (pollen-specific protein-like At4g18596 OS=Cucurbita maxima OX=3661 GN=LOC111486731 PE=4 SV=1)

HSP 1 Score: 338.2 bits (866), Expect = 2.0e-89
Identity = 169/171 (98.83%), Postives = 170/171 (99.42%), Query Frame = 0


Query: 1   MNLFLLFILAFLVPSSLLLGIQASHSHRTRTFSQITVMGLVYCDTCSNNSFSKHSYFLSG 60
           MNLFLLFILAFLVPSSLLLGIQASHSHRTRTF+QITVMGLVYCDTCSNNSFSKHSYFLSG
Sbjct: 1   MNLFLLFILAFLVPSSLLLGIQASHSHRTRTFTQITVMGLVYCDTCSNNSFSKHSYFLSG 60

Query: 61  VEVRISCKFKAETPRTAEQIAFSVNRTTNKYGVYRLEIPSVDGIRCAEDSEIASFCQASL 120
           VEVRISCKFKAETPRTAEQIAFSVNRTTNKYGVYRLEIPSVDGIRCAEDS IASFCQASL
Sbjct: 61  VEVRISCKFKAETPRTAEQIAFSVNRTTNKYGVYRLEIPSVDGIRCAEDSAIASFCQASL 120

Query: 121 IGSPSSSCNVPGYRTTSEEISIKSREANICIYSLNPLNYRPSARDDTLCGN 172
           IGSPSSSCNVPGYRTTSEEISIKSREANICIYSLNPLNYRPSARDDTLCGN
Sbjct: 121 IGSPSSSCNVPGYRTTSEEISIKSREANICIYSLNPLNYRPSARDDTLCGN 171

[Close]

BLAST of Carg15545 vs. ExPASy TrEMBL
Match: A0A6J1DQW0 (major pollen allergen Ole e 1 OS=Momordica charantia OX=3673 GN=LOC111023412 PE=4 SV=1)

HSP 1 Score: 292.7 bits (748), Expect = 9.7e-76
Identity = 145/171 (84.80%), Postives = 154/171 (90.06%), Query Frame = 0


Query: 1   MNLFLLFILAFLVPSSLLLGIQASHSHRTRTFSQITVMGLVYCDTCSNNSFSKHSYFLSG 60
           MNL L F+ AFLVPSSLLL  +A+ S  T T+SQITVMGLVYCDTCSNN+FSKHSYFLSG
Sbjct: 1   MNLILFFVFAFLVPSSLLLESEAARSQPTGTYSQITVMGLVYCDTCSNNTFSKHSYFLSG 60

Query: 61  VEVRISCKFKAETPRTAEQIAFSVNRTTNKYGVYRLEIPSVDGIRCAEDSEIASFCQASL 120
            EVRI CKFKAETPRTAEQIAFSVNRTTNKYGVY+LEIPSVDGIRCAEDS + SFCQASL
Sbjct: 61  AEVRIDCKFKAETPRTAEQIAFSVNRTTNKYGVYKLEIPSVDGIRCAEDSAMVSFCQASL 120

Query: 121 IGSPSSSCNVPGYRTTSEEISIKSREANICIYSLNPLNYRPSARDDTLCGN 172
           IGS SSSCNVPGYR+TS EISIKSR+ANICIYSLNPLNYRPS RDDTLC N
Sbjct: 121 IGSASSSCNVPGYRSTSGEISIKSRQANICIYSLNPLNYRPSTRDDTLCAN 171

[Close]

BLAST of Carg15545 vs. ExPASy TrEMBL
Match: A0A6J1J823 (major pollen allergen Ole e 1-like OS=Cucurbita maxima OX=3661 GN=LOC111482122 PE=4 SV=1)

HSP 1 Score: 291.6 bits (745), Expect = 2.2e-75
Identity = 142/171 (83.04%), Postives = 156/171 (91.23%), Query Frame = 0


Query: 1   MNLFLLFILAFLVPSSLLLGIQASHSHRTRTFSQITVMGLVYCDTCSNNSFSKHSYFLSG 60
           MNL LLF+LA LV SSL+LGIQA+HSH +R FSQITVMGLVYCDTCSNNSFSKHSYFLSG
Sbjct: 1   MNLILLFVLASLVSSSLVLGIQAAHSHHSRIFSQITVMGLVYCDTCSNNSFSKHSYFLSG 60

Query: 61  VEVRISCKFKAETPRTAEQIAFSVNRTTNKYGVYRLEIPSVDGIRCAEDSEIASFCQASL 120
            EVRI CK KAETPRTAEQIAFSVNRTTNK GVY+LEIPS+DGIRCAEDS + SFCQASL
Sbjct: 61  AEVRIDCKLKAETPRTAEQIAFSVNRTTNKNGVYKLEIPSMDGIRCAEDSSMVSFCQASL 120

Query: 121 IGSPSSSCNVPGYRTTSEEISIKSREANICIYSLNPLNYRPSARDDTLCGN 172
           +GS SS+CNVPGY++TS EISIKSR++N+CIYSLNPLNYRPS RDD LCGN
Sbjct: 121 MGSSSSACNVPGYKSTSGEISIKSRQSNVCIYSLNPLNYRPSTRDDILCGN 171

[Close]

BLAST of Carg15545 vs. ExPASy TrEMBL
Match: A0A1S3BPJ6 (major pollen allergen Ole e 1-like OS=Cucumis melo OX=3656 GN=LOC103491809 PE=4 SV=1)

HSP 1 Score: 289.3 bits (739), Expect = 1.1e-74
Identity = 144/172 (83.72%), Postives = 155/172 (90.12%), Query Frame = 0


Query: 1   MNLFLLFILAFLVPSSLLLGIQASHSHRTRT-FSQITVMGLVYCDTCSNNSFSKHSYFLS 60
           MNL LLF+LA LVPSS L  IQA+HSH  RT +SQITVMGLVYCDTCSNN+FSKHSYFLS
Sbjct: 1   MNLILLFVLASLVPSSFLFEIQAAHSHPPRTIYSQITVMGLVYCDTCSNNTFSKHSYFLS 60

Query: 61  GVEVRISCKFKAETPRTAEQIAFSVNRTTNKYGVYRLEIPSVDGIRCAEDSEIASFCQAS 120
           G EVRI CK KAETPRTAEQIAFSVNRTTNKYGVY+LEIPSVDGIRCAEDS + SFCQAS
Sbjct: 61  GAEVRIDCKLKAETPRTAEQIAFSVNRTTNKYGVYKLEIPSVDGIRCAEDSAMVSFCQAS 120

Query: 121 LIGSPSSSCNVPGYRTTSEEISIKSREANICIYSLNPLNYRPSARDDTLCGN 172
           L+GS SSSCNVPGYR+TS EISIKSR++NICIYSLNPLNY+PS RDD LCGN
Sbjct: 121 LMGSSSSSCNVPGYRSTSGEISIKSRQSNICIYSLNPLNYKPSTRDDILCGN 172

[Close]

BLAST of Carg15545 vs. TAIR 10
Match: AT3G26960.1 (Pollen Ole e 1 allergen and extensin family protein )

HSP 1 Score: 179.1 bits (453), Expect = 3.0e-45
Identity = 90/174 (51.72%), Postives = 123/174 (70.69%), Query Frame = 0


Query: 1   MNLFLLFILAFLVPSSLLLGIQASHSH-RTRTFSQITVMGLVYCDTCSNNSFSKHSYFLS 60
           + +FL  +L FL+ +SL     + HS  + +  ++IT+MG VYCD CS NSFSKHSYF+S
Sbjct: 6   LTMFLFILLQFLLVNSL----SSKHSSPKPKPDAEITIMGFVYCDVCSKNSFSKHSYFMS 65

Query: 61  GVEVRISCKFKAETPRTAEQIAFSVNRTTNKYGVYRLEIPSVDGIRCAEDSEIASFCQAS 120
           GVEVRI C+FKA +  T E I FS NRTTN++G+Y++ I S+D   CA+   +AS CQAS
Sbjct: 66  GVEVRIVCRFKAASSTTTETITFSANRTTNEFGLYKVAISSLD---CADVDSLASSCQAS 125

Query: 121 LIGSPS---SSCNVPGYRTTSEEISIKSREANICIYSLNPLNYRPSARDDTLCG 171
           LIG  +   SSCN+PGYRTT++++  KS+++N C+Y  N LN+RP  RD  LCG
Sbjct: 126 LIGRKNFSDSSCNIPGYRTTTDQVLFKSQQSNSCVYGFNALNFRPFKRDLALCG 172

[Close]

BLAST of Carg15545 vs. TAIR 10
Match: AT5G41050.1 (Pollen Ole e 1 allergen and extensin family protein )

HSP 1 Score: 169.5 bits (428), Expect = 2.4e-42
Identity = 90/172 (52.33%), Postives = 119/172 (69.19%), Query Frame = 0


Query: 3   LFLLFILAFLVPSSLLLGIQASHSHRTRTFSQITVMGLVYCDTCSNNSFSKHSYFLSGVE 62
           + LL +L  L  +SL L     HS   +   +ITVMGLVYCD CSNNSFS HSYF+ GVE
Sbjct: 6   IMLLLLLQLLSLNSLSL----KHS-SAKPNGKITVMGLVYCDVCSNNSFSNHSYFIPGVE 65

Query: 63  VRISCKFKAETPRTAEQIAFSVNRTTNKYGVYRLEIPSVDGIRCA----EDSEIASFCQA 122
           VRI C+F + + RT E I FS NRTTN+ G+Y+L+I S++G+ CA    +DS +AS CQA
Sbjct: 66  VRIICRFNSASSRTREMITFSANRTTNELGLYKLDITSLEGVACAAEAKKDSLMAS-CQA 125

Query: 123 SLIGSPSSSCNVPGYRTTSEEISIKSREANICIYSLNPLNYRPSARDDTLCG 171
           SLIG    SCNVPG+RTT+E++  KS+ +N+C+Y    LN+RP  ++  LCG
Sbjct: 126 SLIGRSKDSCNVPGFRTTTEQVVFKSKISNLCVYGFTALNFRPLEKNLHLCG 171

[Close]

BLAST of Carg15545 vs. TAIR 10
Match: AT5G13140.1 (Pollen Ole e 1 allergen and extensin family protein )

HSP 1 Score: 166.4 bits (420), Expect = 2.0e-41
Identity = 83/173 (47.98%), Postives = 122/173 (70.52%), Query Frame = 0


Query: 3   LFLLFILAFLVPSSLLLGI-QASHSHRTRTFSQITVMGLVYCDTCSNNSFSKHSYFLSGV 62
           L+LLF+ + L   +L  G  + S        S+ITV+G+VYCDTCS N+FS+ SYFL GV
Sbjct: 6   LYLLFLASCLTHQALGRGRGRPSPEGSLDPSSRITVVGVVYCDTCSINTFSRQSYFLQGV 65

Query: 63  EVRISCKFKAETPRTAEQIAFSVNRTTNKYGVYRLEIPSVDGIRCAEDSEIASFCQASLI 122
           EV ++C+FKA +P+TAE++  SVNRTTN+ GVY+LEIP VDGI C +   I+S C A ++
Sbjct: 66  EVHVTCRFKASSPKTAEEVNISVNRTTNRSGVYKLEIPHVDGIDCVDGIAISSQCSAKIL 125

Query: 123 GSPS---SSCNVPGYRTTSEEISIKSREANICIYSLNPLNYRPSARDDTLCGN 172
            + S     C++P ++T + E+SIKS++  +CIYSL+ L+Y+P  ++ +LCGN
Sbjct: 126 KTSSDDNGGCSIPVFQTATNEVSIKSKQDRVCIYSLSALSYKPPHKNTSLCGN 178

[Close]

BLAST of Carg15545 vs. TAIR 10
Match: AT5G15780.1 (Pollen Ole e 1 allergen and extensin family protein )

HSP 1 Score: 52.0 bits (123), Expect = 5.5e-07
Identity = 41/163 (25.15%), Postives = 73/163 (44.79%), Query Frame = 0


Query: 3   LFLLFILAFLVPSSLLLGIQASHSH-RTRTFSQITVMGLVYCDTCSNNSFSKH-SYFLSG 62
           L+  F L   +  S+  G+     H   +T S   V+G VYCDTC N +FSK  ++ +SG
Sbjct: 7   LWFWFSLMIFLGISINGGLSQGQQHVMKKTRSSAVVVGTVYCDTCFNGAFSKSPNHLISG 66

Query: 63  VEVRISCKFKAETPRTAEQIAFSVNRTTNKYGVYRLEIPSVDGIRCAEDSEIASFCQASL 122
             V + C  +   P   +++       T+K G +++++P       ++  +    C   L
Sbjct: 67  ALVAVECIDENSKPSFRQEV------KTDKRGEFKVKLP----FSVSKHVKKIKRCSVKL 126

Query: 123 IGSPSSSCNVPGYRTTSEEISIKSRE--ANICIYSLNPLNYRP 162
           + S    C++    T+S    +KS     N  ++S     +RP
Sbjct: 127 LSSSQPYCSIASSATSSSLKRLKSNHHGENTRVFSAGFFTFRP 159

[Close]

BLAST of Carg15545 vs. TAIR 10
Match: AT5G45880.1 (Pollen Ole e 1 allergen and extensin family protein )

HSP 1 Score: 51.6 bits (122), Expect = 7.2e-07
Identity = 43/162 (26.54%), Postives = 72/162 (44.44%), Query Frame = 0


Query: 3   LFLLFILAFLVPSSLLLGIQASHSHRTRTFSQITVMGLVYCDTCSNNSFSKHSYFLSGVE 62
           +F  F +   V  S L G  A+ +     F +  + G VYCDTC     ++ S FL G +
Sbjct: 6   IFFSFFVVSAVCLSSLAGFAAADAD---DFDRFQIQGSVYCDTCRVQFVTRLSKFLEGAK 65

Query: 63  VRISCKFKAETPRTAEQIAFSVNRTTNKYGVYRLEIPSVDGIRCAEDSEIASFCQASLIG 122
           V++ C+      RT   I  +    T+K G Y++E+          D E    C+  L+ 
Sbjct: 66  VKLECR-----SRTNGTITLTKEAVTDKTGSYKMEVTG--------DHE-EEVCELVLVQ 125

Query: 123 SPSSSC---NVPGYRTTSEEISIKSREANICIYS--LNPLNY 160
           SP S C   +   Y   + +IS+ + +  +   +  +NPL +
Sbjct: 126 SPDSGCSDVSTEAYLRNAAKISLTANDGIVSHETRIVNPLGF 150

The following BLAST results are available for this feature:

BLAST of Carg15545 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_022928243.1	3.8e-90	100.00	pollen-specific protein-like At4g18596 [Cucurbita moschata] >XP_023530922.1 poll...	[more]
XP_022989732.1	4.2e-89	98.83	pollen-specific protein-like At4g18596 [Cucurbita maxima]	[more]
KAG6588884.1	1.8e-79	100.00	Glutaredoxin-C1, partial [Cucurbita argyrosperma subsp. sororia]	[more]
XP_038896993.1	2.1e-77	85.38	major pollen allergen Ole e 1-like [Benincasa hispida]	[more]
XP_022156533.1	2.0e-75	84.80	major pollen allergen Ole e 1 [Momordica charantia]	[more]

BLAST of Carg15545 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q6NMJ2	3.0e-05	25.93	Pollen-specific protein-like At4g18596 OS=Arabidopsis thaliana OX=3702 GN=At4g18...	[more]
Q8LGR0	9.5e-04	27.56	Pollen allergen Che a 1 OS=Chenopodium album OX=3559 PE=1 SV=1	[more]

BLAST of Carg15545 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1ENF6	1.8e-90	100.00	pollen-specific protein-like At4g18596 OS=Cucurbita moschata OX=3662 GN=LOC11143...	[more]
A0A6J1JGM9	2.0e-89	98.83	pollen-specific protein-like At4g18596 OS=Cucurbita maxima OX=3661 GN=LOC1114867...	[more]
A0A6J1DQW0	9.7e-76	84.80	major pollen allergen Ole e 1 OS=Momordica charantia OX=3673 GN=LOC111023412 PE=...	[more]
A0A6J1J823	2.2e-75	83.04	major pollen allergen Ole e 1-like OS=Cucurbita maxima OX=3661 GN=LOC111482122 P...	[more]
A0A1S3BPJ6	1.1e-74	83.72	major pollen allergen Ole e 1-like OS=Cucumis melo OX=3656 GN=LOC103491809 PE=4 ...	[more]

BLAST of Carg15545 vs. TAIR 10


Match Name	E-value	Identity	Description
AT3G26960.1	3.0e-45	51.72	Pollen Ole e 1 allergen and extensin family protein	[more]
AT5G41050.1	2.4e-42	52.33	Pollen Ole e 1 allergen and extensin family protein	[more]
AT5G13140.1	2.0e-41	47.98	Pollen Ole e 1 allergen and extensin family protein	[more]
AT5G15780.1	5.5e-07	25.15	Pollen Ole e 1 allergen and extensin family protein	[more]
AT5G45880.1	7.2e-07	26.54	Pollen Ole e 1 allergen and extensin family protein	[more]

InterPro

Analysis Name: InterPro Annotations of Silver-seed gourd (SMH-JMG-627) v2
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
None	No IPR available	PFAM	PF01190	Pollen_Ole_e_1	coord: 37..135 e-value: 1.1E-22 score: 80.3
None	No IPR available	PANTHER	PTHR46995	OS09G0508200 PROTEIN	coord: 3..171

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Carg15545-RA	Carg15545-RA	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
cellular_component	GO:0016021	integral component of membrane