Carg15443 (gene) Silver-seed gourd (SMH-JMG-627) v2

Overview
NameCarg15443
Typegene
OrganismCucurbita argyrosperma subsp. argyrosperma cv. SMH-JMG-627 (Silver-seed gourd (SMH-JMG-627) v2)
Descriptionstress-associated endoplasmic reticulum protein 2-like
LocationCarg_Chr06: 10205418 .. 10205781 (+)
RNA-Seq ExpressionCarg15443
SyntenyCarg15443
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGTAATTTTCCTTTCTTCTTTAATCCATTCTTCTTCCTTACCTTCGATTATTTTCTCCTCTAGGGTTTTGTGTTGCAGATTTGATCTGTGGTTTGATTTTTGGATTTTGTTTCCGGATTTGATGTGATTTTCGTTTTCGATTTATGATTTTGATTTTATGGAAATTTCAGACGACATCACGGCGTTTTGCGGATAGGAAGGTTGAGAGGTTCGAGAAGAACATTTTGAAGAGAGGGGCCGTGCCCGAGACTGCTACGAAGAAGGGAAAGGACTACCCCGTTGGGCCTCTTCTTCTTGGATTCTTCGTTTTTGTCGTCATTGGATCATGTGAGTTTTTCCTTGCTCGCTCTTCGAGATTCTGA

mRNA sequence

ATGACGACATCACGGCGTTTTGCGGATAGGAAGGTTGAGAGGTTCGAGAAGAACATTTTGAAGAGAGGGGCCGTGCCCGAGACTGCTACGAAGAAGGGAAAGGACTACCCCGTTGGGCCTCTTCTTCTTGGATTCTTCGTTTTTGTCGTCATTGGATCATGTGAGTTTTTCCTTGCTCGCTCTTCGAGATTCTGA

Coding sequence (CDS)

ATGACGACATCACGGCGTTTTGCGGATAGGAAGGTTGAGAGGTTCGAGAAGAACATTTTGAAGAGAGGGGCCGTGCCCGAGACTGCTACGAAGAAGGGAAAGGACTACCCCGTTGGGCCTCTTCTTCTTGGATTCTTCGTTTTTGTCGTCATTGGATCATGTGAGTTTTTCCTTGCTCGCTCTTCGAGATTCTGA

Protein sequence

MTTSRRFADRKVERFEKNILKRGAVPETATKKGKDYPVGPLLLGFFVFVVIGSCEFFLARSSRF
Homology
BLAST of Carg15443 vs. NCBI nr
Match: KAG7029119.1 (Stress-associated endoplasmic reticulum protein 2, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 130.2 bits (326), Expect = 6.4e-27
Identity = 64/64 (100.00%), Postives = 64/64 (100.00%), Query Frame = 0

Query: 1  MTTSRRFADRKVERFEKNILKRGAVPETATKKGKDYPVGPLLLGFFVFVVIGSCEFFLAR 60
          MTTSRRFADRKVERFEKNILKRGAVPETATKKGKDYPVGPLLLGFFVFVVIGSCEFFLAR
Sbjct: 1  MTTSRRFADRKVERFEKNILKRGAVPETATKKGKDYPVGPLLLGFFVFVVIGSCEFFLAR 60

Query: 61 SSRF 65
          SSRF
Sbjct: 61 SSRF 64

BLAST of Carg15443 vs. NCBI nr
Match: XP_022932563.1 (stress-associated endoplasmic reticulum protein 2-like isoform X2 [Cucurbita moschata] >XP_022972333.1 stress-associated endoplasmic reticulum protein 2-like [Cucurbita maxima] >XP_023538872.1 stress-associated endoplasmic reticulum protein 2-like [Cucurbita pepo subsp. pepo] >KAG6597676.1 Stress-associated endoplasmic reticulum protein 2, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 110.9 bits (276), Expect = 4.0e-21
Identity = 55/62 (88.71%), Postives = 58/62 (93.55%), Query Frame = 0

Query: 1  MTTSRRFADRKVERFEKNILKRGAVPETATKKGKDYPVGPLLLGFFVFVVIGSCEFFLAR 60
          MTTSRRFADRKVERFEKNILKRGAVPETATKKGKDYPVGPLLLGFFVFVVIGS  F + R
Sbjct: 1  MTTSRRFADRKVERFEKNILKRGAVPETATKKGKDYPVGPLLLGFFVFVVIGSSLFQIIR 60

Query: 61 SS 63
          ++
Sbjct: 61 TA 62

BLAST of Carg15443 vs. NCBI nr
Match: XP_022932562.1 (stress-associated endoplasmic reticulum protein 2-like isoform X1 [Cucurbita moschata])

HSP 1 Score: 109.0 bits (271), Expect = 1.5e-20
Identity = 54/61 (88.52%), Postives = 57/61 (93.44%), Query Frame = 0

Query: 2  TTSRRFADRKVERFEKNILKRGAVPETATKKGKDYPVGPLLLGFFVFVVIGSCEFFLARS 61
          TTSRRFADRKVERFEKNILKRGAVPETATKKGKDYPVGPLLLGFFVFVVIGS  F + R+
Sbjct: 10 TTSRRFADRKVERFEKNILKRGAVPETATKKGKDYPVGPLLLGFFVFVVIGSSLFQIIRT 69

Query: 62 S 63
          +
Sbjct: 70 A 70

BLAST of Carg15443 vs. NCBI nr
Match: XP_038900855.1 (stress-associated endoplasmic reticulum protein 2-like [Benincasa hispida])

HSP 1 Score: 107.8 bits (268), Expect = 3.4e-20
Identity = 53/62 (85.48%), Postives = 57/62 (91.94%), Query Frame = 0

Query: 1  MTTSRRFADRKVERFEKNILKRGAVPETATKKGKDYPVGPLLLGFFVFVVIGSCEFFLAR 60
          MTTSRR ADRKVERFEKNILKRGAVPETATKKGKDYPVGP+LLGFFVFVVIGS  F + R
Sbjct: 1  MTTSRRLADRKVERFEKNILKRGAVPETATKKGKDYPVGPILLGFFVFVVIGSSLFQIIR 60

Query: 61 SS 63
          ++
Sbjct: 61 TA 62

BLAST of Carg15443 vs. NCBI nr
Match: XP_004152134.1 (stress-associated endoplasmic reticulum protein 2 [Cucumis sativus] >KGN53016.1 hypothetical protein Csa_015278 [Cucumis sativus])

HSP 1 Score: 107.8 bits (268), Expect = 3.4e-20
Identity = 53/62 (85.48%), Postives = 57/62 (91.94%), Query Frame = 0

Query: 1  MTTSRRFADRKVERFEKNILKRGAVPETATKKGKDYPVGPLLLGFFVFVVIGSCEFFLAR 60
          MTTSRR ADRKVERFEKNILKRGAVPETATKKGKDYPVGP+LLGFFVFVVIGS  F + R
Sbjct: 1  MTTSRRLADRKVERFEKNILKRGAVPETATKKGKDYPVGPILLGFFVFVVIGSSLFQIIR 60

Query: 61 SS 63
          ++
Sbjct: 61 TA 62

BLAST of Carg15443 vs. ExPASy Swiss-Prot
Match: Q3T073 (Stress-associated endoplasmic reticulum protein 2 OS=Bos taurus OX=9913 GN=SERP2 PE=3 SV=1)

HSP 1 Score: 49.3 bits (116), Expect = 1.9e-05
Identity = 25/51 (49.02%), Postives = 32/51 (62.75%), Query Frame = 0

Query: 13 ERFEKNILKRGAVPETATKKGKDYPVGPLLLGFFVFVVIGSCEFFLARSSR 64
          E+  KNI +RG V +T   + + YPVGP LL  FVFVV GS  F + +S R
Sbjct: 12 EKHSKNITQRGNVAKTLRPQEEKYPVGPWLLALFVFVVCGSAIFQIIQSIR 62

BLAST of Carg15443 vs. ExPASy Swiss-Prot
Match: Q8N6R1 (Stress-associated endoplasmic reticulum protein 2 OS=Homo sapiens OX=9606 GN=SERP2 PE=1 SV=1)

HSP 1 Score: 49.3 bits (116), Expect = 1.9e-05
Identity = 25/51 (49.02%), Postives = 32/51 (62.75%), Query Frame = 0

Query: 13 ERFEKNILKRGAVPETATKKGKDYPVGPLLLGFFVFVVIGSCEFFLARSSR 64
          E+  KNI +RG V +T   + + YPVGP LL  FVFVV GS  F + +S R
Sbjct: 12 EKHSKNITQRGNVAKTLRPQEEKYPVGPWLLALFVFVVCGSAIFQIIQSIR 62

BLAST of Carg15443 vs. ExPASy Swiss-Prot
Match: Q6TAW2 (Stress-associated endoplasmic reticulum protein 2 OS=Mus musculus OX=10090 GN=Serp2 PE=3 SV=2)

HSP 1 Score: 49.3 bits (116), Expect = 1.9e-05
Identity = 25/51 (49.02%), Postives = 32/51 (62.75%), Query Frame = 0

Query: 13 ERFEKNILKRGAVPETATKKGKDYPVGPLLLGFFVFVVIGSCEFFLARSSR 64
          E+  KNI +RG V +T   + + YPVGP LL  FVFVV GS  F + +S R
Sbjct: 12 EKHSKNITQRGNVAKTLRPQEEKYPVGPWLLALFVFVVCGSAIFQIIQSIR 62

BLAST of Carg15443 vs. ExPASy TrEMBL
Match: A0A6J1I9M5 (stress-associated endoplasmic reticulum protein 2-like OS=Cucurbita maxima OX=3661 GN=LOC111470902 PE=3 SV=1)

HSP 1 Score: 110.9 bits (276), Expect = 2.0e-21
Identity = 55/62 (88.71%), Postives = 58/62 (93.55%), Query Frame = 0

Query: 1  MTTSRRFADRKVERFEKNILKRGAVPETATKKGKDYPVGPLLLGFFVFVVIGSCEFFLAR 60
          MTTSRRFADRKVERFEKNILKRGAVPETATKKGKDYPVGPLLLGFFVFVVIGS  F + R
Sbjct: 1  MTTSRRFADRKVERFEKNILKRGAVPETATKKGKDYPVGPLLLGFFVFVVIGSSLFQIIR 60

Query: 61 SS 63
          ++
Sbjct: 61 TA 62

BLAST of Carg15443 vs. ExPASy TrEMBL
Match: A0A6J1F232 (stress-associated endoplasmic reticulum protein 2-like isoform X2 OS=Cucurbita moschata OX=3662 GN=LOC111439082 PE=3 SV=1)

HSP 1 Score: 110.9 bits (276), Expect = 2.0e-21
Identity = 55/62 (88.71%), Postives = 58/62 (93.55%), Query Frame = 0

Query: 1  MTTSRRFADRKVERFEKNILKRGAVPETATKKGKDYPVGPLLLGFFVFVVIGSCEFFLAR 60
          MTTSRRFADRKVERFEKNILKRGAVPETATKKGKDYPVGPLLLGFFVFVVIGS  F + R
Sbjct: 1  MTTSRRFADRKVERFEKNILKRGAVPETATKKGKDYPVGPLLLGFFVFVVIGSSLFQIIR 60

Query: 61 SS 63
          ++
Sbjct: 61 TA 62

BLAST of Carg15443 vs. ExPASy TrEMBL
Match: A0A6J1EX45 (stress-associated endoplasmic reticulum protein 2-like isoform X1 OS=Cucurbita moschata OX=3662 GN=LOC111439082 PE=3 SV=1)

HSP 1 Score: 109.0 bits (271), Expect = 7.4e-21
Identity = 54/61 (88.52%), Postives = 57/61 (93.44%), Query Frame = 0

Query: 2  TTSRRFADRKVERFEKNILKRGAVPETATKKGKDYPVGPLLLGFFVFVVIGSCEFFLARS 61
          TTSRRFADRKVERFEKNILKRGAVPETATKKGKDYPVGPLLLGFFVFVVIGS  F + R+
Sbjct: 10 TTSRRFADRKVERFEKNILKRGAVPETATKKGKDYPVGPLLLGFFVFVVIGSSLFQIIRT 69

Query: 62 S 63
          +
Sbjct: 70 A 70

BLAST of Carg15443 vs. ExPASy TrEMBL
Match: A0A0A0KYW0 (Stress associated endoplasmic reticulum protein OS=Cucumis sativus OX=3659 GN=Csa_4G011630 PE=3 SV=1)

HSP 1 Score: 107.8 bits (268), Expect = 1.7e-20
Identity = 53/62 (85.48%), Postives = 57/62 (91.94%), Query Frame = 0

Query: 1  MTTSRRFADRKVERFEKNILKRGAVPETATKKGKDYPVGPLLLGFFVFVVIGSCEFFLAR 60
          MTTSRR ADRKVERFEKNILKRGAVPETATKKGKDYPVGP+LLGFFVFVVIGS  F + R
Sbjct: 1  MTTSRRLADRKVERFEKNILKRGAVPETATKKGKDYPVGPILLGFFVFVVIGSSLFQIIR 60

Query: 61 SS 63
          ++
Sbjct: 61 TA 62

BLAST of Carg15443 vs. ExPASy TrEMBL
Match: A0A6J1IQU2 (stress-associated endoplasmic reticulum protein 2-like OS=Cucurbita maxima OX=3661 GN=LOC111479167 PE=3 SV=1)

HSP 1 Score: 107.1 bits (266), Expect = 2.8e-20
Identity = 53/62 (85.48%), Postives = 56/62 (90.32%), Query Frame = 0

Query: 1  MTTSRRFADRKVERFEKNILKRGAVPETATKKGKDYPVGPLLLGFFVFVVIGSCEFFLAR 60
          MTTSRR ADRKVERFEKNILKRGAVPET TKKGKDYPVGPLLLGFFVFVVIGS  F + R
Sbjct: 1  MTTSRRLADRKVERFEKNILKRGAVPETTTKKGKDYPVGPLLLGFFVFVVIGSSLFQIIR 60

Query: 61 SS 63
          ++
Sbjct: 61 TA 62

BLAST of Carg15443 vs. TAIR 10
Match: AT1G27350.1 (Ribosome associated membrane protein RAMP4 )

HSP 1 Score: 100.1 bits (248), Expect = 6.6e-22
Identity = 47/62 (75.81%), Postives = 55/62 (88.71%), Query Frame = 0

Query: 1  MTTSRRFADRKVERFEKNILKRGAVPETATKKGKDYPVGPLLLGFFVFVVIGSCEFFLAR 60
          MTTS+R ADRK+E+F+KNILKRG VPET TKKGKDYPVGP+LLGFFVFVVIGS  F + R
Sbjct: 1  MTTSKRLADRKIEKFDKNILKRGFVPETTTKKGKDYPVGPILLGFFVFVVIGSSLFQIIR 60

Query: 61 SS 63
          ++
Sbjct: 61 TA 62

BLAST of Carg15443 vs. TAIR 10
Match: AT1G27330.1 (Ribosome associated membrane protein RAMP4 )

HSP 1 Score: 100.1 bits (248), Expect = 6.6e-22
Identity = 47/62 (75.81%), Postives = 55/62 (88.71%), Query Frame = 0

Query: 1  MTTSRRFADRKVERFEKNILKRGAVPETATKKGKDYPVGPLLLGFFVFVVIGSCEFFLAR 60
          MTTS+R ADRK+E+F+KNILKRG VPET TKKGKDYPVGP+LLGFFVFVVIGS  F + R
Sbjct: 1  MTTSKRLADRKIEKFDKNILKRGFVPETTTKKGKDYPVGPILLGFFVFVVIGSSLFQIIR 60

Query: 61 SS 63
          ++
Sbjct: 61 TA 62

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
KAG7029119.16.4e-27100.00Stress-associated endoplasmic reticulum protein 2, partial [Cucurbita argyrosper... [more]
XP_022932563.14.0e-2188.71stress-associated endoplasmic reticulum protein 2-like isoform X2 [Cucurbita mos... [more]
XP_022932562.11.5e-2088.52stress-associated endoplasmic reticulum protein 2-like isoform X1 [Cucurbita mos... [more]
XP_038900855.13.4e-2085.48stress-associated endoplasmic reticulum protein 2-like [Benincasa hispida][more]
XP_004152134.13.4e-2085.48stress-associated endoplasmic reticulum protein 2 [Cucumis sativus] >KGN53016.1 ... [more]
Match NameE-valueIdentityDescription
Q3T0731.9e-0549.02Stress-associated endoplasmic reticulum protein 2 OS=Bos taurus OX=9913 GN=SERP2... [more]
Q8N6R11.9e-0549.02Stress-associated endoplasmic reticulum protein 2 OS=Homo sapiens OX=9606 GN=SER... [more]
Q6TAW21.9e-0549.02Stress-associated endoplasmic reticulum protein 2 OS=Mus musculus OX=10090 GN=Se... [more]
Match NameE-valueIdentityDescription
A0A6J1I9M52.0e-2188.71stress-associated endoplasmic reticulum protein 2-like OS=Cucurbita maxima OX=36... [more]
A0A6J1F2322.0e-2188.71stress-associated endoplasmic reticulum protein 2-like isoform X2 OS=Cucurbita m... [more]
A0A6J1EX457.4e-2188.52stress-associated endoplasmic reticulum protein 2-like isoform X1 OS=Cucurbita m... [more]
A0A0A0KYW01.7e-2085.48Stress associated endoplasmic reticulum protein OS=Cucumis sativus OX=3659 GN=Cs... [more]
A0A6J1IQU22.8e-2085.48stress-associated endoplasmic reticulum protein 2-like OS=Cucurbita maxima OX=36... [more]
Match NameE-valueIdentityDescription
AT1G27350.16.6e-2275.81Ribosome associated membrane protein RAMP4 [more]
AT1G27330.16.6e-2275.81Ribosome associated membrane protein RAMP4 [more]
InterPro
Analysis Name: InterPro Annotations of Silver-seed gourd (SMH-JMG-627) v2
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR010580Stress-associated endoplasmic reticulum proteinPFAMPF06624RAMP4coord: 5..56
e-value: 8.0E-20
score: 70.6
IPR010580Stress-associated endoplasmic reticulum proteinPANTHERPTHR15601STRESS ASSOCIATED ENDOPLASMIC RETICULUM PROTEIN SERP1/RAMP4coord: 1..62
NoneNo IPR availablePANTHERPTHR15601:SF23OS11G0637501 PROTEINcoord: 1..62

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Carg15443-RACarg15443-RAmRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0030968 endoplasmic reticulum unfolded protein response
biological_process GO:0006486 protein glycosylation
biological_process GO:0015031 protein transport
cellular_component GO:0005783 endoplasmic reticulum
cellular_component GO:0016021 integral component of membrane