Carg15434 (gene) Silver-seed gourd (SMH-JMG-627) v2

Overview
NameCarg15434
Typegene
OrganismCucurbita argyrosperma subsp. argyrosperma cv. SMH-JMG-627 (Silver-seed gourd (SMH-JMG-627) v2)
DescriptionSPARK domain-containing protein
LocationCarg_Chr06: 10245812 .. 10250030 (+)
RNA-Seq ExpressionCarg15434
SyntenyCarg15434
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptidethree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGAGGAAAGATTCTCATTGGATTCGGATCCAACAAAGTCCGGATAAGACCCGATATTTGCCGGTGACCTGGATCCGAATGGGTCAAACAAACCCGCTATTCCCCCTTGACCCGGATCCGAATGGGTCCAACAAAGGTAAAGTTTGTACCTTTTTTCATTCATTTTGCCACTCCCCTCTCTCCCCTGTACCTACCCTCGCTTTTACAACTACAGGGAGCGGGTAATTCTCCTCCCGGCTCTTTCCGGGACGCCGTCCCCCCGCCATTTTTTCTGATTTGGAGCTCCCATCCCTTCAATCTCTCAGCCGATCGGCTCCCAAAAGCATTTCCGGGTGCTCGGGCTGTTGATTCCGAAGCTCCTGCTTGTTTAATTGCACGGTAAACGCAGTTGTTCAATGGGTTCTTCTTCTTCAGGTGTGTTTGCCTTGAACTGAGTTGTTTTCTGTTTGCTTTTCTAGGTTAAAGGTTGTAATGAGTTCCTCTTTTTCTTCTTTTTAGTGAAGCTCGATTTTGGTGCCGTGTGGTTGTTGTCCTGTTTGGTTGAGCTTTCTTTTTGTTTTCTGGAATTATCTTTCGCGTTGAAGTCCTGTTTGTCATGGATTAGCCATTTAAAGATGCTTACGCCGCCGATTCGCTAGAGCTCTAAAAAGGTTGCCATATAACTTGTTTGTGTATAGAGAGAGAGAGATTCATGTGTTGGTTCTGATTGTTTAGGGTTTCTGAATTGTGACTTTCCTGCATTAAATTTTTGTGTGCTTGGTCTTGCATAGTTCGTGTACTTTGGTTTTGTATTGCAGTCTATACTCGACATGTAGCATGTGAATAGTTACCTATTAAACTTGGCATTTCTTCAAGATAATAATCTTTTGAGCTTCACTTTTATTTTTCCAACTTACAATGCAATTCAAATAGTCTTCTCCATTAGATTTGCAATTTCATAGAGGAGTTGTCCCTTTGTTCTGATGGGATGTTAGTAATGCCATCTTTATATTTTGGTTCTTATATTGAACTCCACAAGATCAGTTCAGACTTCAGAGAAAAAAGGAATCATGTTATTGGTCTTCCATGTTTCCTGCACGAAGATTGCTATTCGAATCTGGAAAATATGACAATTGAATTGCATAGTATTTGTGATCATCCTGCAAAATATGGCAGTAGGTGAATTTATAATGGCTGTTATTCGCTTTAAAGCATGCTATAAAATGAAGTGATTCCTTTTCCTATGAGTTATGTTGCCTTTATCTTATGTAGTGTCTTGCTAAATTTAGAACCCTGTGCTTGAAGGTGGTATTGGCATATTTAATATGCTATAGAGCATTTTTCCACGAACTCAGCAAACTACAATTGGGAACTATTTTGCTTACATCATGTCCCCTGGAGGTTAATGGAATGATGGACTGCTTTCGTACTGTAAGCTATCCGAATGGTAGGTCTGCTTTCCCTGCCAAGGCTGGCCTTATCTTTAAGCTAGCATGGAATCTATATTTTAGACGACTCTCTTTCTATTGTTATGTTATTATAATTAAGCACTTCTATTTGTTTTATTGATCTTTGAAATATTATTCAATGCGGAGACGCAATGATCAGGTGTACTAAAATGGAGTACATCTAATTGTCATTTCTTAAGTAGTTCAAAATGATCTAAAATTTTCATGATGGCATCTCTTGTTAACGCATAAGTAATTCCTCGGGATAGTGGCGTATGCAATACTGAATGCTGATTATTACCTAATTTCAGGTAATATGTGCCACCGATTACTATTGTTTGTCGTCTGCATATCCAGTTTTCATGATGGAGTTGTCTTATCTACTCTGGTACAGCATAGTCTTGTTTCTTCCACAAGAGAGCAGGCTCAATCACCTAGAAGTGTTGTCTTTGATCCTATAGAAATATCACCTGCAGTTATCCCACAATACCCTTATCCCAATGATTCTTTGCCTCCAATGTATCCAACTTTTCCTACCAGATATGAACCAGTCTTAACTGGAAGATGCCCTGTAAATTTCTCTGTCATATCAAATGTTATGGACAAAACAGCATCAGATTGTTCTCAGCCCATGGCAGCACTTGTAGGAAATGTTATTTGCTGTCCACAACTAAGTAGCCTGCTCCATATCTTCCAGGGTTTCTATGGCTTAACCTCTGATCGGTTGGTTTTACAAGAGGCAGTTGCTAATGATTGTTTTTCAGATATCATTAGCATCTTAGCCAGCAGAGGAGCGAATCAGACGATACCCTCTCTTTGCTCGGTCAAATCTTCAAATCTTACTGGTGGGTCATGTCCAGTCACAGACGTCGCAACGTTTGAAAAATCAGTTAATGTAAGCAAATTATTAGATGCATGTGATACAGTTGATCCTCTAAAGGAGTGTTGTAGACCAATATGTCAGCCTGCTATCATGGAGGCTGCACTAAAGATTTCTGGAAGGCAATTTTCTATGGATACCAATAGTAACTTGGCAGGGGAGCCGAATCATATTGATTCAATAAACGATTGTAAAAGAGTTGTCTATTCATATATTTCTAGGAAACTCTCCTCCGATGCTGCCAACACTGCATTTCGAATATTATCTGCCTGCAAAGTGAACAAAGGTTAGGTGCTTGTTCATATTTATTGGAATATCAGTTTGTCCATTTTTCTCATAATCTTCTTCCCCTTTCCTAATCATAGAATGTGCTTGTTTTAAAGTATTCCTATTCCATGCCTTATTTGAAATGTGTATGTTCTGTACAGTTTGTCCTTTGGATTTTGAGCAGCCCTCGGAAGTAATTAAAGATTGCCGCAATGTTGCTGCTCCTAATCCCTCATGCTGCAGCTCATTGAACACTTACATTGCTGGGATCCAAAAGCAAATGTTAATTACAAACAAACAAGCTATAATATGTTCAACAATGTTCGGATCCATGTTGCGAAAAGGTGGGGTTATTACAAATGTTTATGAGCTGTGTGATGTCGACTTGAAAGATTTCAGCATTCAAGGTACTGATACTTACTGCATTGTTAATTTAATTTTCCTTCATCGTCGTTGTTTCTATCTTATCTAACATTCCGATTTATTTTGTGCATCGGCGTGGCATCTGAAATATAGCCTATGGACAGCAAGGTTTGCTATTCTTCCCTGTTTTGCACCTTCTTTCTTTTTATTATAGTGATTGATACTCTCTCTCTAAATAATATTTATGTGTTGAAATTTTCTGTCTTCGTTCTTTTTCGAAATTTAGGATGTCTTCTTCGGAGCCTGCCTTCAGATGTGATATTTGACAATTCCACTGGCTTTAGTTTTACCTGTGATTTGACTGATAACATTGCAGCACCGTGGCCTTCGTCGTCCTCAATTTCTACACTGTCTCTTTGTGCGCCCGGTAATTGATAAAAAGGAGACTAATTTTTGCTCTGTTGCTTGTTACACTTAAAAGTTTGACATGAGAGTTTTATTAATCGACAAGTTTGTATTATACTTGCACAGAGATGTCATTGCCTGCTCTACCAACAACAGAGACTAAGAGAAACCATGGTGGGTGCCATTACTCTACGGACTTTTTCTTTGTGAACTATTTTCCTTTTGATATATTCATGATGGTGGGAAGGATAGGATTAAAGGTAGCTCCATCTATGCTGTGGCACAAGCATGTTAAGCTTATCTTGTCTAGCAATCAAATTGGAATGGTCTTGATTTCATATTTATCTTTCTACAAATCATATCAAACTTGCATTAATCTTCAAGGATCCTATGTACATTTGCTTATTAGAGATCTGACTAGTATCTACGACGAGTTTGGTATGTCTTTTTAGGATAGTGAAAACATGCTCTTGGCCTGTTTCTCATATTTTTCATGGTATTTGGTTATATTTTCGACATGATACAACGGGCAATGGCTGAATCATTTCTCGGCTTTTTTTTGTCTATGATCAAGTGTTGTATCTGTTGTTCTTGATCTGATTTGTCCTGTTTTTTAGCATACATTTGATGCAATGGCTGAGTTGTTTGTGTGGAATGATATAATGTGCAGGCCAGCGTGAGGAAGGATTGGATTTCCCAATGCTCATTTTTTCATTTTTTATTATGATCAGTGCATTTTTGTACTGAGTTAGATCTTAGATGAAGATGTTCTGTGGTGTTGTTCAATGTTTGGGGGGCATCTTGGGAATTTTACCCTGGATTTTTGTATGTATATTTTGAGTCTTGTGTATAGTAGTAGCTCTTTTATTATTATCAT

mRNA sequence

ATGAGGAAAGATTCTCATTGGATTCGGATCCAACAAAGTCCGGATAAGACCCGATATTTGCCGGTGACCTGGATCCGAATGGGTCAAACAAACCCGCTATTCCCCCTTGACCCGGATCCGAATGGGTCCAACAAAGCCGATCGGCTCCCAAAAGCATTTCCGGGTGCTCGGGCTGTTGATTCCGAAGCTCCTGCTTGTTTAATTGCACGAACCCTGTGCTTGAAGGTGGTATTGGCATATTTAATATGCTATAGAGCATTTTTCCACGAACTCAGCAAACTACAATTGGGAACTATTTTGCTTACATCATGTCCCCTGGAGGTTAATGGAATGATGGACTGCTTTCGTACTGTAAGCTATCCGAATGGTAATATGTGCCACCGATTACTATTGTTTGTCGTCTGCATATCCAGTTTTCATGATGGAGTTGTCTTATCTACTCTGGTACAGCATAGTCTTGTTTCTTCCACAAGAGAGCAGGCTCAATCACCTAGAAGTGTTGTCTTTGATCCTATAGAAATATCACCTGCAGTTATCCCACAATACCCTTATCCCAATGATTCTTTGCCTCCAATGTATCCAACTTTTCCTACCAGATATGAACCAGTCTTAACTGGAAGATGCCCTGTAAATTTCTCTGTCATATCAAATGTTATGGACAAAACAGCATCAGATTGTTCTCAGCCCATGGCAGCACTTGTAGGAAATGTTATTTGCTGTCCACAACTAAGTAGCCTGCTCCATATCTTCCAGGGTTTCTATGGCTTAACCTCTGATCGGTTGGTTTTACAAGAGGCAGTTGCTAATGATTGTTTTTCAGATATCATTAGCATCTTAGCCAGCAGAGGAGCGAATCAGACGATACCCTCTCTTTGCTCGGTCAAATCTTCAAATCTTACTGGTGGGTCATGTCCAGTCACAGACGTCGCAACGTTTGAAAAATCAGTTAATGTAAGCAAATTATTAGATGCATGTGATACAGTTGATCCTCTAAAGGAGTGTTGTAGACCAATATGTCAGCCTGCTATCATGGAGGCTGCACTAAAGATTTCTGGAAGGCAATTTTCTATGGATACCAATAGTAACTTGGCAGGGGAGCCGAATCATATTGATTCAATAAACGATTGTAAAAGAGTTGTCTATTCATATATTTCTAGGAAACTCTCCTCCGATGCTGCCAACACTGCATTTCGAATATTATCTGCCTGCAAAGTGAACAAAGTTTGTCCTTTGGATTTTGAGCAGCCCTCGGAAGTAATTAAAGATTGCCGCAATGTTGCTGCTCCTAATCCCTCATGCTGCAGCTCATTGAACACTTACATTGCTGGGATCCAAAAGCAAATGTTAATTACAAACAAACAAGCTATAATATGTTCAACAATGTTCGGATCCATGTTGCGAAAAGGTGGGGTTATTACAAATGTTTATGAGCTGTGTGATGTCGACTTGAAAGATTTCAGCATTCAAGGTACTGATACTTACTGCATTGTTAATTTAATTTTCCTTCATCGTCGATGTCTTCTTCGGAGCCTGCCTTCAGATGTGATATTTGACAATTCCACTGGCTTTAGTTTTACCTGTGATTTGACTGATAACATTGCAGCACCGTGGCCTTCGTCGTCCTCAATTTCTACACTGTCTCTTTGTGCGCCCGAGATGTCATTGCCTGCTCTACCAACAACAGAGACTAAGAGAAACCATGGTGGGTGCCATTACTCTACGGACTTTTTCTTTGTGAACTATTTTCCTTTTGATATATTCATGATGGTGGGAAGGATAGGATTAAAGGTAGCTCCATCTATGCTGTGGCACAAGCATGTTAAGCTTATCTTGTCTAGCAATCAAATTGGAATGGTCTTGATTTCATATTTATCTTTCTACAAATCATATCAAACTTGCATTAATCTTCAAGGATCCTATGTACATTTGCTTATTAGAGATCTGACTAGTATCTACGACGAGTTTGGTATGTCTTTTTAGGATAGTGAAAACATGCTCTTGGCCTGTTTCTCATATTTTTCATGGTATTTGGTTATATTTTCGACATGATACAACGGGCAATGGCTGAATCATTTCTCGGCTTTTTTTTGTCTATGATCAAGTGTTGTATCTGTTGTTCTTGATCTGATTTGTCCTGTTTTTTAGCATACATTTGATGCAATGGCTGAGTTGTTTGTGTGGAATGATATAATGTGCAGGCCAGCGTGAGGAAGGATTGGATTTCCCAATGCTCATTTTTTCATTTTTTATTATGATCAGTGCATTTTTGTACTGAGTTAGATCTTAGATGAAGATGTTCTGTGGTGTTGTTCAATGTTTGGGGGGCATCTTGGGAATTTTACCCTGGATTTTTGTATGTATATTTTGAGTCTTGTGTATAGTAGTAGCTCTTTTATTATTATCAT

Coding sequence (CDS)

ATGAGGAAAGATTCTCATTGGATTCGGATCCAACAAAGTCCGGATAAGACCCGATATTTGCCGGTGACCTGGATCCGAATGGGTCAAACAAACCCGCTATTCCCCCTTGACCCGGATCCGAATGGGTCCAACAAAGCCGATCGGCTCCCAAAAGCATTTCCGGGTGCTCGGGCTGTTGATTCCGAAGCTCCTGCTTGTTTAATTGCACGAACCCTGTGCTTGAAGGTGGTATTGGCATATTTAATATGCTATAGAGCATTTTTCCACGAACTCAGCAAACTACAATTGGGAACTATTTTGCTTACATCATGTCCCCTGGAGGTTAATGGAATGATGGACTGCTTTCGTACTGTAAGCTATCCGAATGGTAATATGTGCCACCGATTACTATTGTTTGTCGTCTGCATATCCAGTTTTCATGATGGAGTTGTCTTATCTACTCTGGTACAGCATAGTCTTGTTTCTTCCACAAGAGAGCAGGCTCAATCACCTAGAAGTGTTGTCTTTGATCCTATAGAAATATCACCTGCAGTTATCCCACAATACCCTTATCCCAATGATTCTTTGCCTCCAATGTATCCAACTTTTCCTACCAGATATGAACCAGTCTTAACTGGAAGATGCCCTGTAAATTTCTCTGTCATATCAAATGTTATGGACAAAACAGCATCAGATTGTTCTCAGCCCATGGCAGCACTTGTAGGAAATGTTATTTGCTGTCCACAACTAAGTAGCCTGCTCCATATCTTCCAGGGTTTCTATGGCTTAACCTCTGATCGGTTGGTTTTACAAGAGGCAGTTGCTAATGATTGTTTTTCAGATATCATTAGCATCTTAGCCAGCAGAGGAGCGAATCAGACGATACCCTCTCTTTGCTCGGTCAAATCTTCAAATCTTACTGGTGGGTCATGTCCAGTCACAGACGTCGCAACGTTTGAAAAATCAGTTAATGTAAGCAAATTATTAGATGCATGTGATACAGTTGATCCTCTAAAGGAGTGTTGTAGACCAATATGTCAGCCTGCTATCATGGAGGCTGCACTAAAGATTTCTGGAAGGCAATTTTCTATGGATACCAATAGTAACTTGGCAGGGGAGCCGAATCATATTGATTCAATAAACGATTGTAAAAGAGTTGTCTATTCATATATTTCTAGGAAACTCTCCTCCGATGCTGCCAACACTGCATTTCGAATATTATCTGCCTGCAAAGTGAACAAAGTTTGTCCTTTGGATTTTGAGCAGCCCTCGGAAGTAATTAAAGATTGCCGCAATGTTGCTGCTCCTAATCCCTCATGCTGCAGCTCATTGAACACTTACATTGCTGGGATCCAAAAGCAAATGTTAATTACAAACAAACAAGCTATAATATGTTCAACAATGTTCGGATCCATGTTGCGAAAAGGTGGGGTTATTACAAATGTTTATGAGCTGTGTGATGTCGACTTGAAAGATTTCAGCATTCAAGGTACTGATACTTACTGCATTGTTAATTTAATTTTCCTTCATCGTCGATGTCTTCTTCGGAGCCTGCCTTCAGATGTGATATTTGACAATTCCACTGGCTTTAGTTTTACCTGTGATTTGACTGATAACATTGCAGCACCGTGGCCTTCGTCGTCCTCAATTTCTACACTGTCTCTTTGTGCGCCCGAGATGTCATTGCCTGCTCTACCAACAACAGAGACTAAGAGAAACCATGGTGGGTGCCATTACTCTACGGACTTTTTCTTTGTGAACTATTTTCCTTTTGATATATTCATGATGGTGGGAAGGATAGGATTAAAGGTAGCTCCATCTATGCTGTGGCACAAGCATGTTAAGCTTATCTTGTCTAGCAATCAAATTGGAATGGTCTTGATTTCATATTTATCTTTCTACAAATCATATCAAACTTGCATTAATCTTCAAGGATCCTATGTACATTTGCTTATTAGAGATCTGACTAGTATCTACGACGAGTTTGGTATGTCTTTTTAG

Protein sequence

MRKDSHWIRIQQSPDKTRYLPVTWIRMGQTNPLFPLDPDPNGSNKADRLPKAFPGARAVDSEAPACLIARTLCLKVVLAYLICYRAFFHELSKLQLGTILLTSCPLEVNGMMDCFRTVSYPNGNMCHRLLLFVVCISSFHDGVVLSTLVQHSLVSSTREQAQSPRSVVFDPIEISPAVIPQYPYPNDSLPPMYPTFPTRYEPVLTGRCPVNFSVISNVMDKTASDCSQPMAALVGNVICCPQLSSLLHIFQGFYGLTSDRLVLQEAVANDCFSDIISILASRGANQTIPSLCSVKSSNLTGGSCPVTDVATFEKSVNVSKLLDACDTVDPLKECCRPICQPAIMEAALKISGRQFSMDTNSNLAGEPNHIDSINDCKRVVYSYISRKLSSDAANTAFRILSACKVNKVCPLDFEQPSEVIKDCRNVAAPNPSCCSSLNTYIAGIQKQMLITNKQAIICSTMFGSMLRKGGVITNVYELCDVDLKDFSIQGTDTYCIVNLIFLHRRCLLRSLPSDVIFDNSTGFSFTCDLTDNIAAPWPSSSSISTLSLCAPEMSLPALPTTETKRNHGGCHYSTDFFFVNYFPFDIFMMVGRIGLKVAPSMLWHKHVKLILSSNQIGMVLISYLSFYKSYQTCINLQGSYVHLLIRDLTSIYDEFGMSF
Homology
BLAST of Carg15434 vs. NCBI nr
Match: KAG7029128.1 (putative GPI-anchored protein, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 1333.5 bits (3450), Expect = 0.0e+00
Identity = 659/659 (100.00%), Postives = 659/659 (100.00%), Query Frame = 0

Query: 1   MRKDSHWIRIQQSPDKTRYLPVTWIRMGQTNPLFPLDPDPNGSNKADRLPKAFPGARAVD 60
           MRKDSHWIRIQQSPDKTRYLPVTWIRMGQTNPLFPLDPDPNGSNKADRLPKAFPGARAVD
Sbjct: 1   MRKDSHWIRIQQSPDKTRYLPVTWIRMGQTNPLFPLDPDPNGSNKADRLPKAFPGARAVD 60

Query: 61  SEAPACLIARTLCLKVVLAYLICYRAFFHELSKLQLGTILLTSCPLEVNGMMDCFRTVSY 120
           SEAPACLIARTLCLKVVLAYLICYRAFFHELSKLQLGTILLTSCPLEVNGMMDCFRTVSY
Sbjct: 61  SEAPACLIARTLCLKVVLAYLICYRAFFHELSKLQLGTILLTSCPLEVNGMMDCFRTVSY 120

Query: 121 PNGNMCHRLLLFVVCISSFHDGVVLSTLVQHSLVSSTREQAQSPRSVVFDPIEISPAVIP 180
           PNGNMCHRLLLFVVCISSFHDGVVLSTLVQHSLVSSTREQAQSPRSVVFDPIEISPAVIP
Sbjct: 121 PNGNMCHRLLLFVVCISSFHDGVVLSTLVQHSLVSSTREQAQSPRSVVFDPIEISPAVIP 180

Query: 181 QYPYPNDSLPPMYPTFPTRYEPVLTGRCPVNFSVISNVMDKTASDCSQPMAALVGNVICC 240
           QYPYPNDSLPPMYPTFPTRYEPVLTGRCPVNFSVISNVMDKTASDCSQPMAALVGNVICC
Sbjct: 181 QYPYPNDSLPPMYPTFPTRYEPVLTGRCPVNFSVISNVMDKTASDCSQPMAALVGNVICC 240

Query: 241 PQLSSLLHIFQGFYGLTSDRLVLQEAVANDCFSDIISILASRGANQTIPSLCSVKSSNLT 300
           PQLSSLLHIFQGFYGLTSDRLVLQEAVANDCFSDIISILASRGANQTIPSLCSVKSSNLT
Sbjct: 241 PQLSSLLHIFQGFYGLTSDRLVLQEAVANDCFSDIISILASRGANQTIPSLCSVKSSNLT 300

Query: 301 GGSCPVTDVATFEKSVNVSKLLDACDTVDPLKECCRPICQPAIMEAALKISGRQFSMDTN 360
           GGSCPVTDVATFEKSVNVSKLLDACDTVDPLKECCRPICQPAIMEAALKISGRQFSMDTN
Sbjct: 301 GGSCPVTDVATFEKSVNVSKLLDACDTVDPLKECCRPICQPAIMEAALKISGRQFSMDTN 360

Query: 361 SNLAGEPNHIDSINDCKRVVYSYISRKLSSDAANTAFRILSACKVNKVCPLDFEQPSEVI 420
           SNLAGEPNHIDSINDCKRVVYSYISRKLSSDAANTAFRILSACKVNKVCPLDFEQPSEVI
Sbjct: 361 SNLAGEPNHIDSINDCKRVVYSYISRKLSSDAANTAFRILSACKVNKVCPLDFEQPSEVI 420

Query: 421 KDCRNVAAPNPSCCSSLNTYIAGIQKQMLITNKQAIICSTMFGSMLRKGGVITNVYELCD 480
           KDCRNVAAPNPSCCSSLNTYIAGIQKQMLITNKQAIICSTMFGSMLRKGGVITNVYELCD
Sbjct: 421 KDCRNVAAPNPSCCSSLNTYIAGIQKQMLITNKQAIICSTMFGSMLRKGGVITNVYELCD 480

Query: 481 VDLKDFSIQGTDTYCIVNLIFLHRRCLLRSLPSDVIFDNSTGFSFTCDLTDNIAAPWPSS 540
           VDLKDFSIQGTDTYCIVNLIFLHRRCLLRSLPSDVIFDNSTGFSFTCDLTDNIAAPWPSS
Sbjct: 481 VDLKDFSIQGTDTYCIVNLIFLHRRCLLRSLPSDVIFDNSTGFSFTCDLTDNIAAPWPSS 540

Query: 541 SSISTLSLCAPEMSLPALPTTETKRNHGGCHYSTDFFFVNYFPFDIFMMVGRIGLKVAPS 600
           SSISTLSLCAPEMSLPALPTTETKRNHGGCHYSTDFFFVNYFPFDIFMMVGRIGLKVAPS
Sbjct: 541 SSISTLSLCAPEMSLPALPTTETKRNHGGCHYSTDFFFVNYFPFDIFMMVGRIGLKVAPS 600

Query: 601 MLWHKHVKLILSSNQIGMVLISYLSFYKSYQTCINLQGSYVHLLIRDLTSIYDEFGMSF 660
           MLWHKHVKLILSSNQIGMVLISYLSFYKSYQTCINLQGSYVHLLIRDLTSIYDEFGMSF
Sbjct: 601 MLWHKHVKLILSSNQIGMVLISYLSFYKSYQTCINLQGSYVHLLIRDLTSIYDEFGMSF 659

BLAST of Carg15434 vs. NCBI nr
Match: KAG6597683.1 (putative GPI-anchored protein, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 953.0 bits (2462), Expect = 1.4e-273
Identity = 484/519 (93.26%), Postives = 489/519 (94.22%), Query Frame = 0

Query: 72  LCLKVVLAYLICYRAFFHELSKLQLGTILLTSCPLEVNGMMDCFRTVSYPNGNMCHRLLL 131
           L L+VVLAYLICYRAFFHELSKLQLGTILLTSCPLEVNGMMDCFRTVSYPNGNMCHRLLL
Sbjct: 47  LLLQVVLAYLICYRAFFHELSKLQLGTILLTSCPLEVNGMMDCFRTVSYPNGNMCHRLLL 106

Query: 132 FVVCISSFHDGVVLSTLVQHSLVSSTREQAQSPRSVVFDPIEISPAVIPQYPYPNDSLPP 191
           FVVCISSFHDGVVLSTLVQHSLVSSTREQAQSPRSVVFDPIEISPAVIPQYPYPNDSLPP
Sbjct: 107 FVVCISSFHDGVVLSTLVQHSLVSSTREQAQSPRSVVFDPIEISPAVIPQYPYPNDSLPP 166

Query: 192 MYPTFPTRYEPVLTGRCPVNFSVISNVMDKTASDCSQPMAALVGNVICCPQLSSLLHIFQ 251
           MYPTFPTRYEPVLTGRCPVNFSVISNVMDKTASDCSQPMAALVGNVICCPQLSSLLHIFQ
Sbjct: 167 MYPTFPTRYEPVLTGRCPVNFSVISNVMDKTASDCSQPMAALVGNVICCPQLSSLLHIFQ 226

Query: 252 GFYGLTSDRLVLQEAVANDCFSDIISILASRGANQTIPSLCSVKSSNLTGGSCPVTDVAT 311
           GFYGLTSDRLVLQEAVANDCFSDIISILASRGANQTIPSLCSVKSSNLTGGSCPVTDVAT
Sbjct: 227 GFYGLTSDRLVLQEAVANDCFSDIISILASRGANQTIPSLCSVKSSNLTGGSCPVTDVAT 286

Query: 312 FEKSVNVSKLLDACDTVDPLKECCRPICQPAIMEAALKISGRQFSMDTNSNLAGEPNHID 371
           FEKSVNVSKLLDACDTVDPLKECCRPICQPAIMEAALKISGRQFSMDTNSNLAGEPNHID
Sbjct: 287 FEKSVNVSKLLDACDTVDPLKECCRPICQPAIMEAALKISGRQFSMDTNSNLAGEPNHID 346

Query: 372 SINDCKRVVYSYISRKLSSDAANTAFRILSACKVNKVCPLDFEQPSEVIKDCRNVAAPNP 431
           SINDCKRVVYSYISRKLSSDAANTAFRILSACKVNKVCPLDFEQPSEVIKDCRNVAAPNP
Sbjct: 347 SINDCKRVVYSYISRKLSSDAANTAFRILSACKVNKVCPLDFEQPSEVIKDCRNVAAPNP 406

Query: 432 SCCSSLNTYIAGIQKQMLITNKQAIICSTMFGSMLRKGGVITNVYELCDVDLKDFSIQGT 491
           SCCSSLNTYIAGIQKQMLITNKQAIICSTMFGSMLRKGGVITNVYELCDVDLKDFSIQ  
Sbjct: 407 SCCSSLNTYIAGIQKQMLITNKQAIICSTMFGSMLRKGGVITNVYELCDVDLKDFSIQA- 466

Query: 492 DTYCIVNLIFLHRRCLLRSLPSDVIFDNSTGFSFTCDLTDNIAAPWPSSSSISTLSLCAP 551
                    +  + CLLRSLPSDVIFDNSTGFSFTCDLTDNIAAPWPSSSSISTLSLCAP
Sbjct: 467 ---------YGQQGCLLRSLPSDVIFDNSTGFSFTCDLTDNIAAPWPSSSSISTLSLCAP 526

Query: 552 EMSLPALPTTETKRNHGGCHYSTDFFFVNYFPFDIFMMV 591
           EMSLPALPTTETKRNHG      DF       F  F+M+
Sbjct: 527 EMSLPALPTTETKRNHGQREEGLDF---PMLIFSFFIMI 552

BLAST of Carg15434 vs. NCBI nr
Match: XP_022932740.1 (uncharacterized GPI-anchored protein At1g61900 [Cucurbita moschata] >XP_022932741.1 uncharacterized GPI-anchored protein At1g61900 [Cucurbita moschata])

HSP 1 Score: 873.2 bits (2255), Expect = 1.4e-249
Identity = 443/480 (92.29%), Postives = 449/480 (93.54%), Query Frame = 0

Query: 111 MMDCFRTVSYPNGNMCHRLLLFVVCISSFHDGVVLSTLVQHSLVSSTREQAQSPRSVVFD 170
           MMDCFRTVSYPNGNMCHRLLLFVV ISSFHDGVVLSTLVQHSLVSSTREQAQSP SVVFD
Sbjct: 1   MMDCFRTVSYPNGNMCHRLLLFVVWISSFHDGVVLSTLVQHSLVSSTREQAQSPSSVVFD 60

Query: 171 PIEISPAVIPQYPYPNDSLPPMYPTFPTRYEPVLTGRCPVNFSVISNVMDKTASDCSQPM 230
           PIEISPAVIPQYPYPNDSLPPMYPTFPTRYEPVLTGRCPVNFSVISNVMDKTASDCSQPM
Sbjct: 61  PIEISPAVIPQYPYPNDSLPPMYPTFPTRYEPVLTGRCPVNFSVISNVMDKTASDCSQPM 120

Query: 231 AALVGNVICCPQLSSLLHIFQGFYGLTSDRLVLQEAVANDCFSDIISILASRGANQTIPS 290
           AALVGNVICCPQLSSLLHIFQGFYGLTSDRLVLQEAVANDCFSDIISILASRGANQTIPS
Sbjct: 121 AALVGNVICCPQLSSLLHIFQGFYGLTSDRLVLQEAVANDCFSDIISILASRGANQTIPS 180

Query: 291 LCSVKSSNLTGGSCPVTDVATFEKSVNVSKLLDACDTVDPLKECCRPICQPAIMEAALKI 350
           LCSVKSSNLTGGSCPVTDVATFEKSVNVSKLLDACDTVDPLKECCRPICQPAIMEAAL+I
Sbjct: 181 LCSVKSSNLTGGSCPVTDVATFEKSVNVSKLLDACDTVDPLKECCRPICQPAIMEAALQI 240

Query: 351 SGRQFSMDTNSNLAGEPNHIDSINDCKRVVYSYISRKLSSDAANTAFRILSACKVNKVCP 410
           SGRQFSMDTN+NLAGEPNHIDSINDCKRVVYSYISRKLSSDAANTAFRILSACKVNKVCP
Sbjct: 241 SGRQFSMDTNNNLAGEPNHIDSINDCKRVVYSYISRKLSSDAANTAFRILSACKVNKVCP 300

Query: 411 LDFEQPSEVIKDCRNVAAPNPSCCSSLNTYIAGIQKQMLITNKQAIICSTMFGSMLRKGG 470
           LDFEQPSEVIKDCRNVAAPNPSCCSSLNTYIAGIQKQMLITNKQAIICSTMFGSMLRKGG
Sbjct: 301 LDFEQPSEVIKDCRNVAAPNPSCCSSLNTYIAGIQKQMLITNKQAIICSTMFGSMLRKGG 360

Query: 471 VITNVYELCDVDLKDFSIQGTDTYCIVNLIFLHRRCLLRSLPSDVIFDNSTGFSFTCDLT 530
           VITNVYELCDVDLKDFSIQ           +  + CLLRSLPSDVIFDNSTGFSFTCDLT
Sbjct: 361 VITNVYELCDVDLKDFSIQA----------YGQQGCLLRSLPSDVIFDNSTGFSFTCDLT 420

Query: 531 DNIAAPWPSSSSISTLSLCAPEMSLPALPTTETKRNHGGCHYSTDFFFVNYFPFDIFMMV 590
           DNIAAPWPSSSSISTLSLCAPEMSLPALPTTETKRNHG      DF       F  F+M+
Sbjct: 421 DNIAAPWPSSSSISTLSLCAPEMSLPALPTTETKRNHGQREEGLDF---PMLIFSFFIMI 467

BLAST of Carg15434 vs. NCBI nr
Match: XP_023540156.1 (uncharacterized GPI-anchored protein At1g61900-like [Cucurbita pepo subsp. pepo] >XP_023540157.1 uncharacterized GPI-anchored protein At1g61900-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 870.9 bits (2249), Expect = 6.9e-249
Identity = 442/480 (92.08%), Postives = 448/480 (93.33%), Query Frame = 0

Query: 111 MMDCFRTVSYPNGNMCHRLLLFVVCISSFHDGVVLSTLVQHSLVSSTREQAQSPRSVVFD 170
           MMDCFRTVSYPNGNMCHRLLLFVV ISSFHDGVVLSTLVQHS+VSST EQAQSP SVVFD
Sbjct: 1   MMDCFRTVSYPNGNMCHRLLLFVVWISSFHDGVVLSTLVQHSIVSSTGEQAQSPSSVVFD 60

Query: 171 PIEISPAVIPQYPYPNDSLPPMYPTFPTRYEPVLTGRCPVNFSVISNVMDKTASDCSQPM 230
           PIEISPAVIPQYPYPNDSLPPMYPTFPTRYEPVLTGRCPVNFSVISNVMDKTASDCSQPM
Sbjct: 61  PIEISPAVIPQYPYPNDSLPPMYPTFPTRYEPVLTGRCPVNFSVISNVMDKTASDCSQPM 120

Query: 231 AALVGNVICCPQLSSLLHIFQGFYGLTSDRLVLQEAVANDCFSDIISILASRGANQTIPS 290
           AALVGNVICCPQLSSLLHIFQGFYGLTSDRLVLQEAVANDCFSDIISILASRGANQTIPS
Sbjct: 121 AALVGNVICCPQLSSLLHIFQGFYGLTSDRLVLQEAVANDCFSDIISILASRGANQTIPS 180

Query: 291 LCSVKSSNLTGGSCPVTDVATFEKSVNVSKLLDACDTVDPLKECCRPICQPAIMEAALKI 350
           LCSVKSSNLTGGSCPVTDVATFEKSVNVSKLLDACDTVDPLKECCRPICQPAIMEAAL+I
Sbjct: 181 LCSVKSSNLTGGSCPVTDVATFEKSVNVSKLLDACDTVDPLKECCRPICQPAIMEAALQI 240

Query: 351 SGRQFSMDTNSNLAGEPNHIDSINDCKRVVYSYISRKLSSDAANTAFRILSACKVNKVCP 410
           SGRQFSMDTNSNLAGEPNHIDSINDCKRVVYSYISRKLSSDAANTAFRILSACKVNKVCP
Sbjct: 241 SGRQFSMDTNSNLAGEPNHIDSINDCKRVVYSYISRKLSSDAANTAFRILSACKVNKVCP 300

Query: 411 LDFEQPSEVIKDCRNVAAPNPSCCSSLNTYIAGIQKQMLITNKQAIICSTMFGSMLRKGG 470
           LDFEQPSEVIKDCRNVAAPNPSCCSSLNTYIAGIQKQMLITNKQAIICSTMFGSMLRKGG
Sbjct: 301 LDFEQPSEVIKDCRNVAAPNPSCCSSLNTYIAGIQKQMLITNKQAIICSTMFGSMLRKGG 360

Query: 471 VITNVYELCDVDLKDFSIQGTDTYCIVNLIFLHRRCLLRSLPSDVIFDNSTGFSFTCDLT 530
           VITNVYELCDVDLKDFSIQ           +  + CLLRSLPSDVIFDNSTGFSFTCDLT
Sbjct: 361 VITNVYELCDVDLKDFSIQA----------YGQQGCLLRSLPSDVIFDNSTGFSFTCDLT 420

Query: 531 DNIAAPWPSSSSISTLSLCAPEMSLPALPTTETKRNHGGCHYSTDFFFVNYFPFDIFMMV 590
           DNIAAPWPSSSSISTLSLCAPEMSLPALPTTETKRNHG      DF       F  F+M+
Sbjct: 421 DNIAAPWPSSSSISTLSLCAPEMSLPALPTTETKRNHGQREEGLDF---PMLIFSFFIMI 467

BLAST of Carg15434 vs. NCBI nr
Match: XP_022972312.1 (uncharacterized GPI-anchored protein At1g61900-like [Cucurbita maxima] >XP_022972313.1 uncharacterized GPI-anchored protein At1g61900-like [Cucurbita maxima])

HSP 1 Score: 865.1 bits (2234), Expect = 3.8e-247
Identity = 439/480 (91.46%), Postives = 446/480 (92.92%), Query Frame = 0

Query: 111 MMDCFRTVSYPNGNMCHRLLLFVVCISSFHDGVVLSTLVQHSLVSSTREQAQSPRSVVFD 170
           MMDCFRTVSYPNGNMCHRLLLFVV ISSFHDGVVLSTLVQHSLVSSTREQAQSP SVVFD
Sbjct: 1   MMDCFRTVSYPNGNMCHRLLLFVVWISSFHDGVVLSTLVQHSLVSSTREQAQSPSSVVFD 60

Query: 171 PIEISPAVIPQYPYPNDSLPPMYPTFPTRYEPVLTGRCPVNFSVISNVMDKTASDCSQPM 230
           PIEISPAVIPQYPYPNDSLPPMYPTFPTRYEPVLTGRCPVNFSVISNVMDKTASDCSQPM
Sbjct: 61  PIEISPAVIPQYPYPNDSLPPMYPTFPTRYEPVLTGRCPVNFSVISNVMDKTASDCSQPM 120

Query: 231 AALVGNVICCPQLSSLLHIFQGFYGLTSDRLVLQEAVANDCFSDIISILASRGANQTIPS 290
           AALVGNVICCPQLSSLLHIFQGFYGLTSDRLVLQEAVANDCFSDIISILASRGANQTIPS
Sbjct: 121 AALVGNVICCPQLSSLLHIFQGFYGLTSDRLVLQEAVANDCFSDIISILASRGANQTIPS 180

Query: 291 LCSVKSSNLTGGSCPVTDVATFEKSVNVSKLLDACDTVDPLKECCRPICQPAIMEAALKI 350
           LCSVKSSNLTGGSCPVTDVATFEKSVNVSKLLDACDTVDPLKECCRPICQPAIMEAAL+I
Sbjct: 181 LCSVKSSNLTGGSCPVTDVATFEKSVNVSKLLDACDTVDPLKECCRPICQPAIMEAALQI 240

Query: 351 SGRQFSMDTNSNLAGEPNHIDSINDCKRVVYSYISRKLSSDAANTAFRILSACKVNKVCP 410
           SGRQFSMDTNSNLA EPNHIDSINDCKRVVYSYISRKLSSD AN AFRILSACKVNKVCP
Sbjct: 241 SGRQFSMDTNSNLAREPNHIDSINDCKRVVYSYISRKLSSDTANAAFRILSACKVNKVCP 300

Query: 411 LDFEQPSEVIKDCRNVAAPNPSCCSSLNTYIAGIQKQMLITNKQAIICSTMFGSMLRKGG 470
           LDFEQPSEVIKDCRNVAAPNPSCCSSLNTYIAGIQKQMLITNKQAIIC+TMFGSMLRKGG
Sbjct: 301 LDFEQPSEVIKDCRNVAAPNPSCCSSLNTYIAGIQKQMLITNKQAIICATMFGSMLRKGG 360

Query: 471 VITNVYELCDVDLKDFSIQGTDTYCIVNLIFLHRRCLLRSLPSDVIFDNSTGFSFTCDLT 530
           VITNVYELCDVDLKDFSIQ           +  + CLLRSLPSDVIFDNSTGFSFTCDLT
Sbjct: 361 VITNVYELCDVDLKDFSIQA----------YGQQGCLLRSLPSDVIFDNSTGFSFTCDLT 420

Query: 531 DNIAAPWPSSSSISTLSLCAPEMSLPALPTTETKRNHGGCHYSTDFFFVNYFPFDIFMMV 590
           DNIAAPWPSSSSISTLSLCAPEMSLPALPTTETKRNHG      +F       F  F+M+
Sbjct: 421 DNIAAPWPSSSSISTLSLCAPEMSLPALPTTETKRNHGQREEGLEF---PMLIFSFFIMI 467

BLAST of Carg15434 vs. ExPASy Swiss-Prot
Match: Q8GUI4 (Uncharacterized GPI-anchored protein At1g61900 OS=Arabidopsis thaliana OX=3702 GN=At1g61900 PE=2 SV=1)

HSP 1 Score: 290.0 bits (741), Expect = 6.6e-77
Identity = 165/423 (39.01%), Postives = 235/423 (55.56%), Query Frame = 0

Query: 143 VVLSTLVQHSLVSSTREQAQSPRSVVFDPIEISPAVIPQYPYPNDSLPPMYPTFPTRYEP 202
           V+L ++   SL SS + +   P        EISP   PQ   P  +  PM P +     P
Sbjct: 13  VILQSMFLISLCSSQKPEEFLP--------EISPDTSPQPFLPFIAPSPMVP-YINSTMP 72

Query: 203 VLTGRCPVNFSVISNVMDKTASDCSQPMAALVGNVICCPQLSSLLHIFQGFYGLTSDRLV 262
            L+G C +NFS   +++  T+ +C    A L+ NV+CCPQL + L I  G     +  L 
Sbjct: 73  KLSGLCSLNFSASESLIQTTSHNCWTVFAPLLANVMCCPQLDATLTIILGKASKETGLLA 132

Query: 263 LQEAVANDCFSDIISILASRGANQTIPSLCSVKSSNLTGGSCPVTDVATFEKSVNVSKLL 322
           L    +  C SD+  IL  +GA+  +  +CS+ SSNLT  SCPV +V  FE +V+ +KLL
Sbjct: 133 LNRTQSKHCLSDLEQILVGKGASGQLNKICSIHSSNLTSSSCPVINVDEFESTVDTAKLL 192

Query: 323 DACDTVDPLKECCRPICQPAIMEAALKISGRQFSMDTNSNLAGEPNHIDSINDCKRVVYS 382
            AC+ +DP+KECC   CQ AI++AA  IS +     T+++        D INDCK VV  
Sbjct: 193 LACEKIDPVKECCEEACQNAILDAATNISLKASETLTDNS--------DRINDCKNVVNR 252

Query: 383 YISRKLSSDAANTAFRILSACKVNKVCPLDFEQPSEVIKDCRNVAAPNPSCCSSLNTYIA 442
           +++ KL         R L+ CK+N+VCPL F     +  +C N  +    CC ++ +Y++
Sbjct: 253 WLATKLDPSRVKETLRGLANCKINRVCPLVFPHMKHIGGNCSNELSNQTGCCRAMESYVS 312

Query: 443 GIQKQMLITNKQAIICSTMFGSMLRKGGVITNVYELCDVDLKDFSIQGTDTYCIVNLIFL 502
            +QKQ LITN QA+ C+T  G+ L+K  +  N++ +C + LKDFS+Q  +          
Sbjct: 313 HLQKQTLITNLQALDCATSLGTKLQKLNITKNIFSVCHISLKDFSLQVGNQ--------- 372

Query: 503 HRRCLLRSLPSDVIFDNSTGFSFTCDLTDNIAAPWPSSSSISTLSLCAPEMSLPALPTTE 562
              CLL SLPSD IFD  TG SFTCDL DNI APWP SSS+S+ S C   + +PALP   
Sbjct: 373 ESGCLLPSLPSDAIFDKDTGISFTCDLNDNIPAPWP-SSSLSSASTCKKPVRIPALPAAA 408

Query: 563 TKR 566
           + +
Sbjct: 433 SSQ 408

BLAST of Carg15434 vs. ExPASy TrEMBL
Match: A0A6J1F2Y9 (uncharacterized GPI-anchored protein At1g61900 OS=Cucurbita moschata OX=3662 GN=LOC111439199 PE=4 SV=1)

HSP 1 Score: 873.2 bits (2255), Expect = 6.7e-250
Identity = 443/480 (92.29%), Postives = 449/480 (93.54%), Query Frame = 0

Query: 111 MMDCFRTVSYPNGNMCHRLLLFVVCISSFHDGVVLSTLVQHSLVSSTREQAQSPRSVVFD 170
           MMDCFRTVSYPNGNMCHRLLLFVV ISSFHDGVVLSTLVQHSLVSSTREQAQSP SVVFD
Sbjct: 1   MMDCFRTVSYPNGNMCHRLLLFVVWISSFHDGVVLSTLVQHSLVSSTREQAQSPSSVVFD 60

Query: 171 PIEISPAVIPQYPYPNDSLPPMYPTFPTRYEPVLTGRCPVNFSVISNVMDKTASDCSQPM 230
           PIEISPAVIPQYPYPNDSLPPMYPTFPTRYEPVLTGRCPVNFSVISNVMDKTASDCSQPM
Sbjct: 61  PIEISPAVIPQYPYPNDSLPPMYPTFPTRYEPVLTGRCPVNFSVISNVMDKTASDCSQPM 120

Query: 231 AALVGNVICCPQLSSLLHIFQGFYGLTSDRLVLQEAVANDCFSDIISILASRGANQTIPS 290
           AALVGNVICCPQLSSLLHIFQGFYGLTSDRLVLQEAVANDCFSDIISILASRGANQTIPS
Sbjct: 121 AALVGNVICCPQLSSLLHIFQGFYGLTSDRLVLQEAVANDCFSDIISILASRGANQTIPS 180

Query: 291 LCSVKSSNLTGGSCPVTDVATFEKSVNVSKLLDACDTVDPLKECCRPICQPAIMEAALKI 350
           LCSVKSSNLTGGSCPVTDVATFEKSVNVSKLLDACDTVDPLKECCRPICQPAIMEAAL+I
Sbjct: 181 LCSVKSSNLTGGSCPVTDVATFEKSVNVSKLLDACDTVDPLKECCRPICQPAIMEAALQI 240

Query: 351 SGRQFSMDTNSNLAGEPNHIDSINDCKRVVYSYISRKLSSDAANTAFRILSACKVNKVCP 410
           SGRQFSMDTN+NLAGEPNHIDSINDCKRVVYSYISRKLSSDAANTAFRILSACKVNKVCP
Sbjct: 241 SGRQFSMDTNNNLAGEPNHIDSINDCKRVVYSYISRKLSSDAANTAFRILSACKVNKVCP 300

Query: 411 LDFEQPSEVIKDCRNVAAPNPSCCSSLNTYIAGIQKQMLITNKQAIICSTMFGSMLRKGG 470
           LDFEQPSEVIKDCRNVAAPNPSCCSSLNTYIAGIQKQMLITNKQAIICSTMFGSMLRKGG
Sbjct: 301 LDFEQPSEVIKDCRNVAAPNPSCCSSLNTYIAGIQKQMLITNKQAIICSTMFGSMLRKGG 360

Query: 471 VITNVYELCDVDLKDFSIQGTDTYCIVNLIFLHRRCLLRSLPSDVIFDNSTGFSFTCDLT 530
           VITNVYELCDVDLKDFSIQ           +  + CLLRSLPSDVIFDNSTGFSFTCDLT
Sbjct: 361 VITNVYELCDVDLKDFSIQA----------YGQQGCLLRSLPSDVIFDNSTGFSFTCDLT 420

Query: 531 DNIAAPWPSSSSISTLSLCAPEMSLPALPTTETKRNHGGCHYSTDFFFVNYFPFDIFMMV 590
           DNIAAPWPSSSSISTLSLCAPEMSLPALPTTETKRNHG      DF       F  F+M+
Sbjct: 421 DNIAAPWPSSSSISTLSLCAPEMSLPALPTTETKRNHGQREEGLDF---PMLIFSFFIMI 467

BLAST of Carg15434 vs. ExPASy TrEMBL
Match: A0A6J1I4H0 (uncharacterized GPI-anchored protein At1g61900-like OS=Cucurbita maxima OX=3661 GN=LOC111470888 PE=4 SV=1)

HSP 1 Score: 865.1 bits (2234), Expect = 1.8e-247
Identity = 439/480 (91.46%), Postives = 446/480 (92.92%), Query Frame = 0

Query: 111 MMDCFRTVSYPNGNMCHRLLLFVVCISSFHDGVVLSTLVQHSLVSSTREQAQSPRSVVFD 170
           MMDCFRTVSYPNGNMCHRLLLFVV ISSFHDGVVLSTLVQHSLVSSTREQAQSP SVVFD
Sbjct: 1   MMDCFRTVSYPNGNMCHRLLLFVVWISSFHDGVVLSTLVQHSLVSSTREQAQSPSSVVFD 60

Query: 171 PIEISPAVIPQYPYPNDSLPPMYPTFPTRYEPVLTGRCPVNFSVISNVMDKTASDCSQPM 230
           PIEISPAVIPQYPYPNDSLPPMYPTFPTRYEPVLTGRCPVNFSVISNVMDKTASDCSQPM
Sbjct: 61  PIEISPAVIPQYPYPNDSLPPMYPTFPTRYEPVLTGRCPVNFSVISNVMDKTASDCSQPM 120

Query: 231 AALVGNVICCPQLSSLLHIFQGFYGLTSDRLVLQEAVANDCFSDIISILASRGANQTIPS 290
           AALVGNVICCPQLSSLLHIFQGFYGLTSDRLVLQEAVANDCFSDIISILASRGANQTIPS
Sbjct: 121 AALVGNVICCPQLSSLLHIFQGFYGLTSDRLVLQEAVANDCFSDIISILASRGANQTIPS 180

Query: 291 LCSVKSSNLTGGSCPVTDVATFEKSVNVSKLLDACDTVDPLKECCRPICQPAIMEAALKI 350
           LCSVKSSNLTGGSCPVTDVATFEKSVNVSKLLDACDTVDPLKECCRPICQPAIMEAAL+I
Sbjct: 181 LCSVKSSNLTGGSCPVTDVATFEKSVNVSKLLDACDTVDPLKECCRPICQPAIMEAALQI 240

Query: 351 SGRQFSMDTNSNLAGEPNHIDSINDCKRVVYSYISRKLSSDAANTAFRILSACKVNKVCP 410
           SGRQFSMDTNSNLA EPNHIDSINDCKRVVYSYISRKLSSD AN AFRILSACKVNKVCP
Sbjct: 241 SGRQFSMDTNSNLAREPNHIDSINDCKRVVYSYISRKLSSDTANAAFRILSACKVNKVCP 300

Query: 411 LDFEQPSEVIKDCRNVAAPNPSCCSSLNTYIAGIQKQMLITNKQAIICSTMFGSMLRKGG 470
           LDFEQPSEVIKDCRNVAAPNPSCCSSLNTYIAGIQKQMLITNKQAIIC+TMFGSMLRKGG
Sbjct: 301 LDFEQPSEVIKDCRNVAAPNPSCCSSLNTYIAGIQKQMLITNKQAIICATMFGSMLRKGG 360

Query: 471 VITNVYELCDVDLKDFSIQGTDTYCIVNLIFLHRRCLLRSLPSDVIFDNSTGFSFTCDLT 530
           VITNVYELCDVDLKDFSIQ           +  + CLLRSLPSDVIFDNSTGFSFTCDLT
Sbjct: 361 VITNVYELCDVDLKDFSIQA----------YGQQGCLLRSLPSDVIFDNSTGFSFTCDLT 420

Query: 531 DNIAAPWPSSSSISTLSLCAPEMSLPALPTTETKRNHGGCHYSTDFFFVNYFPFDIFMMV 590
           DNIAAPWPSSSSISTLSLCAPEMSLPALPTTETKRNHG      +F       F  F+M+
Sbjct: 421 DNIAAPWPSSSSISTLSLCAPEMSLPALPTTETKRNHGQREEGLEF---PMLIFSFFIMI 467

BLAST of Carg15434 vs. ExPASy TrEMBL
Match: A0A6J1IQC3 (uncharacterized GPI-anchored protein At1g61900-like isoform X2 OS=Cucurbita maxima OX=3661 GN=LOC111479050 PE=4 SV=1)

HSP 1 Score: 842.0 bits (2174), Expect = 1.7e-240
Identity = 423/497 (85.11%), Postives = 445/497 (89.54%), Query Frame = 0

Query: 75  KVVLAYLICYRAFFHELSKLQLGTILLTSCPLEVNGMMDCFRTVSYP--NGNMCHRLLLF 134
           KV LAYLICYRAF         G   L  CPLE  GMMDCF TVSY   NGNMCHRLLL 
Sbjct: 11  KVALAYLICYRAFSTNSEDYDRGLFCLRRCPLEAYGMMDCFHTVSYSYLNGNMCHRLLLL 70

Query: 135 VVCISSFHDGVVLSTLVQHSLVSSTREQAQSPRSVVFDPIEISPAVIPQYPYPNDSLPPM 194
           V+CISSFHDGV L+T+VQ  LV S REQAQSP SVVFDPIEISPAVIPQYPYPN+SLPPM
Sbjct: 71  VICISSFHDGVALATVVQRRLVLSMREQAQSPSSVVFDPIEISPAVIPQYPYPNESLPPM 130

Query: 195 YPTFPTRYEPVLTGRCPVNFSVISNVMDKTASDCSQPMAALVGNVICCPQLSSLLHIFQG 254
           YPTFPTRYEPVLTGRCPVNFSVISN MDKTASDCSQPMAALVGN ICCPQLSSLLHIFQG
Sbjct: 131 YPTFPTRYEPVLTGRCPVNFSVISNAMDKTASDCSQPMAALVGNAICCPQLSSLLHIFQG 190

Query: 255 FYGLTSDRLVLQEAVANDCFSDIISILASRGANQTIPSLCSVKSSNLTGGSCPVTDVATF 314
           FYGLTS++LVLQEAVANDCFSDIISILASRGANQTIPSLCSVKSSNLTGGSCPVTDVA F
Sbjct: 191 FYGLTSEQLVLQEAVANDCFSDIISILASRGANQTIPSLCSVKSSNLTGGSCPVTDVAMF 250

Query: 315 EKSVNVSKLLDACDTVDPLKECCRPICQPAIMEAALKISGRQFSMDTNSNLAGEPNHIDS 374
           EK VNVSKLLDACDTVDPLKECCRPICQPAIMEAAL+ISG QFS DT+SN+A +PNH+DS
Sbjct: 251 EKLVNVSKLLDACDTVDPLKECCRPICQPAIMEAALQISGGQFSTDTSSNVAAQPNHMDS 310

Query: 375 INDCKRVVYSYISRKLSSDAANTAFRILSACKVNKVCPLDFEQPSEVIKDCRNVAAPNPS 434
           INDCKRVVYSYISRKLSSDAANTAFRILS+CKVNK CPLDFEQPSEVIKDCRNVAAP+PS
Sbjct: 311 INDCKRVVYSYISRKLSSDAANTAFRILSSCKVNKACPLDFEQPSEVIKDCRNVAAPSPS 370

Query: 435 CCSSLNTYIAGIQKQMLITNKQAIICSTMFGSMLRKGGVITNVYELCDVDLKDFSIQGTD 494
           CC SLN+YIAGIQKQMLITNKQAIIC+TMFGSMLRKGGV+TNVYELCDVDLKDFSIQ   
Sbjct: 371 CCKSLNSYIAGIQKQMLITNKQAIICATMFGSMLRKGGVMTNVYELCDVDLKDFSIQA-- 430

Query: 495 TYCIVNLIFLHRRCLLRSLPSDVIFDNSTGFSFTCDLTDNIAAPWPSSSSISTLSLCAPE 554
                   +  + CLLRSLPSD+IFD STGFSFTCDLTDNIAAPWPSSSSIS+LSLCAPE
Sbjct: 431 --------YGQQGCLLRSLPSDLIFDKSTGFSFTCDLTDNIAAPWPSSSSISSLSLCAPE 490

Query: 555 MSLPALPTTETKRNHGG 570
           MSLPALPT+ETKRN+GG
Sbjct: 491 MSLPALPTSETKRNNGG 497

BLAST of Carg15434 vs. ExPASy TrEMBL
Match: A0A6J1GUV6 (uncharacterized GPI-anchored protein At1g61900-like isoform X2 OS=Cucurbita moschata OX=3662 GN=LOC111457653 PE=4 SV=1)

HSP 1 Score: 842.0 bits (2174), Expect = 1.7e-240
Identity = 423/497 (85.11%), Postives = 445/497 (89.54%), Query Frame = 0

Query: 75  KVVLAYLICYRAFFHELSKLQLGTILLTSCPLEVNGMMDCFRTVSYP--NGNMCHRLLLF 134
           KV LAYLICYRAF         G   L  CPLE  GMMDCF TVSY   NGNMCHRLLL 
Sbjct: 11  KVALAYLICYRAFSTNSEDYNRGLFCLRQCPLEAYGMMDCFHTVSYSYLNGNMCHRLLLL 70

Query: 135 VVCISSFHDGVVLSTLVQHSLVSSTREQAQSPRSVVFDPIEISPAVIPQYPYPNDSLPPM 194
           V+CISSFHDGV L+T+VQ  LV   REQAQSP SVVFDPIEISPAVIPQYPYPN+SLPPM
Sbjct: 71  VICISSFHDGVALATVVQRRLVLPMREQAQSPSSVVFDPIEISPAVIPQYPYPNESLPPM 130

Query: 195 YPTFPTRYEPVLTGRCPVNFSVISNVMDKTASDCSQPMAALVGNVICCPQLSSLLHIFQG 254
           YPTFPTRYEPVLTGRCPVNFSVISNVMDKTASDCSQPMAALVGN ICCPQLSSLLHIFQG
Sbjct: 131 YPTFPTRYEPVLTGRCPVNFSVISNVMDKTASDCSQPMAALVGNAICCPQLSSLLHIFQG 190

Query: 255 FYGLTSDRLVLQEAVANDCFSDIISILASRGANQTIPSLCSVKSSNLTGGSCPVTDVATF 314
           FYGLTS++LVLQEAVANDCFSDIISILASRGANQTIPSLCSVKSSNLTGGSCPVTDVATF
Sbjct: 191 FYGLTSEQLVLQEAVANDCFSDIISILASRGANQTIPSLCSVKSSNLTGGSCPVTDVATF 250

Query: 315 EKSVNVSKLLDACDTVDPLKECCRPICQPAIMEAALKISGRQFSMDTNSNLAGEPNHIDS 374
           EK VNVSKLLDACDTVDPLKECCRPICQPAIMEAAL+ISG Q S DT+SN+A +PNHIDS
Sbjct: 251 EKLVNVSKLLDACDTVDPLKECCRPICQPAIMEAALQISGGQISTDTSSNVAAQPNHIDS 310

Query: 375 INDCKRVVYSYISRKLSSDAANTAFRILSACKVNKVCPLDFEQPSEVIKDCRNVAAPNPS 434
           INDCKRVVYSYISRKLSSDAANTAFRILS+CKVNK CPLDFEQPSEVIKDCRNVAAP+P+
Sbjct: 311 INDCKRVVYSYISRKLSSDAANTAFRILSSCKVNKACPLDFEQPSEVIKDCRNVAAPSPA 370

Query: 435 CCSSLNTYIAGIQKQMLITNKQAIICSTMFGSMLRKGGVITNVYELCDVDLKDFSIQGTD 494
           CC SLN+YIAGIQKQMLITNKQAIIC+TMFGSMLRKGGV+TNVYELCDVDLKDFSIQ   
Sbjct: 371 CCKSLNSYIAGIQKQMLITNKQAIICATMFGSMLRKGGVMTNVYELCDVDLKDFSIQA-- 430

Query: 495 TYCIVNLIFLHRRCLLRSLPSDVIFDNSTGFSFTCDLTDNIAAPWPSSSSISTLSLCAPE 554
                   +  + CLLRSLPSD+IFD STGFSFTCDLTDNIAAPWPSSSSIS+LSLCAPE
Sbjct: 431 --------YGQQGCLLRSLPSDLIFDKSTGFSFTCDLTDNIAAPWPSSSSISSLSLCAPE 490

Query: 555 MSLPALPTTETKRNHGG 570
           MSLPALPT+ETKRN+GG
Sbjct: 491 MSLPALPTSETKRNNGG 497

BLAST of Carg15434 vs. ExPASy TrEMBL
Match: A0A6J1IST1 (uncharacterized GPI-anchored protein At1g61900-like isoform X1 OS=Cucurbita maxima OX=3661 GN=LOC111479050 PE=4 SV=1)

HSP 1 Score: 840.1 bits (2169), Expect = 6.3e-240
Identity = 422/496 (85.08%), Postives = 444/496 (89.52%), Query Frame = 0

Query: 76  VVLAYLICYRAFFHELSKLQLGTILLTSCPLEVNGMMDCFRTVSYP--NGNMCHRLLLFV 135
           V LAYLICYRAF         G   L  CPLE  GMMDCF TVSY   NGNMCHRLLL V
Sbjct: 13  VALAYLICYRAFSTNSEDYDRGLFCLRRCPLEAYGMMDCFHTVSYSYLNGNMCHRLLLLV 72

Query: 136 VCISSFHDGVVLSTLVQHSLVSSTREQAQSPRSVVFDPIEISPAVIPQYPYPNDSLPPMY 195
           +CISSFHDGV L+T+VQ  LV S REQAQSP SVVFDPIEISPAVIPQYPYPN+SLPPMY
Sbjct: 73  ICISSFHDGVALATVVQRRLVLSMREQAQSPSSVVFDPIEISPAVIPQYPYPNESLPPMY 132

Query: 196 PTFPTRYEPVLTGRCPVNFSVISNVMDKTASDCSQPMAALVGNVICCPQLSSLLHIFQGF 255
           PTFPTRYEPVLTGRCPVNFSVISN MDKTASDCSQPMAALVGN ICCPQLSSLLHIFQGF
Sbjct: 133 PTFPTRYEPVLTGRCPVNFSVISNAMDKTASDCSQPMAALVGNAICCPQLSSLLHIFQGF 192

Query: 256 YGLTSDRLVLQEAVANDCFSDIISILASRGANQTIPSLCSVKSSNLTGGSCPVTDVATFE 315
           YGLTS++LVLQEAVANDCFSDIISILASRGANQTIPSLCSVKSSNLTGGSCPVTDVA FE
Sbjct: 193 YGLTSEQLVLQEAVANDCFSDIISILASRGANQTIPSLCSVKSSNLTGGSCPVTDVAMFE 252

Query: 316 KSVNVSKLLDACDTVDPLKECCRPICQPAIMEAALKISGRQFSMDTNSNLAGEPNHIDSI 375
           K VNVSKLLDACDTVDPLKECCRPICQPAIMEAAL+ISG QFS DT+SN+A +PNH+DSI
Sbjct: 253 KLVNVSKLLDACDTVDPLKECCRPICQPAIMEAALQISGGQFSTDTSSNVAAQPNHMDSI 312

Query: 376 NDCKRVVYSYISRKLSSDAANTAFRILSACKVNKVCPLDFEQPSEVIKDCRNVAAPNPSC 435
           NDCKRVVYSYISRKLSSDAANTAFRILS+CKVNK CPLDFEQPSEVIKDCRNVAAP+PSC
Sbjct: 313 NDCKRVVYSYISRKLSSDAANTAFRILSSCKVNKACPLDFEQPSEVIKDCRNVAAPSPSC 372

Query: 436 CSSLNTYIAGIQKQMLITNKQAIICSTMFGSMLRKGGVITNVYELCDVDLKDFSIQGTDT 495
           C SLN+YIAGIQKQMLITNKQAIIC+TMFGSMLRKGGV+TNVYELCDVDLKDFSIQ    
Sbjct: 373 CKSLNSYIAGIQKQMLITNKQAIICATMFGSMLRKGGVMTNVYELCDVDLKDFSIQA--- 432

Query: 496 YCIVNLIFLHRRCLLRSLPSDVIFDNSTGFSFTCDLTDNIAAPWPSSSSISTLSLCAPEM 555
                  +  + CLLRSLPSD+IFD STGFSFTCDLTDNIAAPWPSSSSIS+LSLCAPEM
Sbjct: 433 -------YGQQGCLLRSLPSDLIFDKSTGFSFTCDLTDNIAAPWPSSSSISSLSLCAPEM 492

Query: 556 SLPALPTTETKRNHGG 570
           SLPALPT+ETKRN+GG
Sbjct: 493 SLPALPTSETKRNNGG 498

BLAST of Carg15434 vs. TAIR 10
Match: AT2G30700.1 (unknown protein; BEST Arabidopsis thaliana protein match is: unknown protein (TAIR:AT1G61900.1); Has 68 Blast hits to 67 proteins in 13 species: Archae - 0; Bacteria - 2; Metazoa - 0; Fungi - 0; Plants - 66; Viruses - 0; Other Eukaryotes - 0 (source: NCBI BLink). )

HSP 1 Score: 609.0 bits (1569), Expect = 4.5e-174
Identity = 309/482 (64.11%), Postives = 372/482 (77.18%), Query Frame = 0

Query: 112 MDCFRTVSYPNGNMCHRLLLFVVCISSFHDGVVLSTLVQHSLVSSTREQAQSPRSVVFDP 171
           M    TV +  G + +R LLF++ +SSF D      L +HS  S+T E A  P   V  P
Sbjct: 8   MGSLETVCWLKGCLVYRFLLFIIWLSSFQDVAAHDKLNEHSSRSTTSELANPPGIGVSGP 67

Query: 172 IEISPAVIPQYPYPN-DSLPPMYPTFPTRYEPVLTGRCPVNFSVISNVMDKTASDCSQPM 231
           I++SP+VIP+Y  P     PPMYPTFP  YEP LTG+CP +F  IS+V+D  ASDCSQP 
Sbjct: 68  IQVSPSVIPKYASPALPWTPPMYPTFPDTYEPKLTGKCPTDFQAISSVIDTAASDCSQPF 127

Query: 232 AALVGNVICCPQLSSLLHIFQGFYGLTSDRLVLQEAVANDCFSDIISILASRGANQTIPS 291
           AALVGNVICCPQ  SLLHIFQG + + S++LVL +AVA DCFSDI+SIL SR AN TIP+
Sbjct: 128 AALVGNVICCPQFVSLLHIFQGQHNVKSNKLVLPDAVATDCFSDIVSILVSRRANMTIPA 187

Query: 292 LCSVKSSNLTGGSCPVTDVATFEKSVNVSKLLDACDTVDPLKECCRPICQPAIMEAALKI 351
           LCSV SSNLTGGSCPVTDV TFEK VN SKLLDAC TVDPLKECCRPICQPAIMEAAL I
Sbjct: 188 LCSVTSSNLTGGSCPVTDVTTFEKVVNSSKLLDACRTVDPLKECCRPICQPAIMEAALII 247

Query: 352 SGRQFSMDTNSNLAGEPNHIDSINDCKRVVYSYISRKLSSDAANTAFRILSACKVNKVCP 411
           SG Q ++     LAG  N++++INDCK VV+SY+SRKL +D AN AFRILS+CKVNK CP
Sbjct: 248 SGHQMTVGDKIPLAGS-NNVNAINDCKNVVFSYLSRKLPADKANAAFRILSSCKVNKACP 307

Query: 412 LDFEQPSEVIKDCRNVAAPNPSCCSSLNTYIAGIQKQMLITNKQAIICSTMFGSMLRKGG 471
           L+F++P+EVIK CRNVAAP+PSCCSSLN YI+GIQ QMLITNKQAI+C+T+ GSMLRKGG
Sbjct: 308 LEFKEPTEVIKACRNVAAPSPSCCSSLNAYISGIQNQMLITNKQAIVCATVIGSMLRKGG 367

Query: 472 VITNVYELCDVDLKDFSIQGTDTYCIVNLIFLHRRCLLRSLPSDVIFDNSTGFSFTCDLT 531
           V+TN+YELCDVDLKDFS+Q            + + CLLRS P+D+IFDN++G+SFTCDLT
Sbjct: 368 VMTNIYELCDVDLKDFSVQAYG---------MQQGCLLRSYPADLIFDNTSGYSFTCDLT 427

Query: 532 DNIAAPWPSSSSISTLSLCAPEMSLPALPTTETKRNHGGCHYSTDFFFVNYFPFDIFMMV 591
           DNIAAPWPSSSS+S+LSLCAPEMSLPALPT++T +NHG C+       +    F ++++V
Sbjct: 428 DNIAAPWPSSSSMSSLSLCAPEMSLPALPTSQTIKNHGFCNGGVGALRLIVLVFLLYVVV 479

Query: 592 GR 593
            R
Sbjct: 488 VR 479

BLAST of Carg15434 vs. TAIR 10
Match: AT1G61900.1 (unknown protein; FUNCTIONS IN: molecular_function unknown; INVOLVED IN: biological_process unknown; LOCATED IN: anchored to plasma membrane, plasma membrane, anchored to membrane; EXPRESSED IN: 23 plant structures; EXPRESSED DURING: 13 growth stages; BEST Arabidopsis thaliana protein match is: unknown protein (TAIR:AT2G30700.1); Has 65 Blast hits to 65 proteins in 12 species: Archae - 0; Bacteria - 0; Metazoa - 0; Fungi - 0; Plants - 65; Viruses - 0; Other Eukaryotes - 0 (source: NCBI BLink). )

HSP 1 Score: 290.0 bits (741), Expect = 4.7e-78
Identity = 165/423 (39.01%), Postives = 235/423 (55.56%), Query Frame = 0

Query: 143 VVLSTLVQHSLVSSTREQAQSPRSVVFDPIEISPAVIPQYPYPNDSLPPMYPTFPTRYEP 202
           V+L ++   SL SS + +   P        EISP   PQ   P  +  PM P +     P
Sbjct: 13  VILQSMFLISLCSSQKPEEFLP--------EISPDTSPQPFLPFIAPSPMVP-YINSTMP 72

Query: 203 VLTGRCPVNFSVISNVMDKTASDCSQPMAALVGNVICCPQLSSLLHIFQGFYGLTSDRLV 262
            L+G C +NFS   +++  T+ +C    A L+ NV+CCPQL + L I  G     +  L 
Sbjct: 73  KLSGLCSLNFSASESLIQTTSHNCWTVFAPLLANVMCCPQLDATLTIILGKASKETGLLA 132

Query: 263 LQEAVANDCFSDIISILASRGANQTIPSLCSVKSSNLTGGSCPVTDVATFEKSVNVSKLL 322
           L    +  C SD+  IL  +GA+  +  +CS+ SSNLT  SCPV +V  FE +V+ +KLL
Sbjct: 133 LNRTQSKHCLSDLEQILVGKGASGQLNKICSIHSSNLTSSSCPVINVDEFESTVDTAKLL 192

Query: 323 DACDTVDPLKECCRPICQPAIMEAALKISGRQFSMDTNSNLAGEPNHIDSINDCKRVVYS 382
            AC+ +DP+KECC   CQ AI++AA  IS +     T+++        D INDCK VV  
Sbjct: 193 LACEKIDPVKECCEEACQNAILDAATNISLKASETLTDNS--------DRINDCKNVVNR 252

Query: 383 YISRKLSSDAANTAFRILSACKVNKVCPLDFEQPSEVIKDCRNVAAPNPSCCSSLNTYIA 442
           +++ KL         R L+ CK+N+VCPL F     +  +C N  +    CC ++ +Y++
Sbjct: 253 WLATKLDPSRVKETLRGLANCKINRVCPLVFPHMKHIGGNCSNELSNQTGCCRAMESYVS 312

Query: 443 GIQKQMLITNKQAIICSTMFGSMLRKGGVITNVYELCDVDLKDFSIQGTDTYCIVNLIFL 502
            +QKQ LITN QA+ C+T  G+ L+K  +  N++ +C + LKDFS+Q  +          
Sbjct: 313 HLQKQTLITNLQALDCATSLGTKLQKLNITKNIFSVCHISLKDFSLQVGNQ--------- 372

Query: 503 HRRCLLRSLPSDVIFDNSTGFSFTCDLTDNIAAPWPSSSSISTLSLCAPEMSLPALPTTE 562
              CLL SLPSD IFD  TG SFTCDL DNI APWP SSS+S+ S C   + +PALP   
Sbjct: 373 ESGCLLPSLPSDAIFDKDTGISFTCDLNDNIPAPWP-SSSLSSASTCKKPVRIPALPAAA 408

Query: 563 TKR 566
           + +
Sbjct: 433 SSQ 408

BLAST of Carg15434 vs. TAIR 10
Match: AT1G61900.3 (unknown protein; BEST Arabidopsis thaliana protein match is: unknown protein (TAIR:AT2G30700.1). )

HSP 1 Score: 290.0 bits (741), Expect = 4.7e-78
Identity = 165/423 (39.01%), Postives = 235/423 (55.56%), Query Frame = 0

Query: 143 VVLSTLVQHSLVSSTREQAQSPRSVVFDPIEISPAVIPQYPYPNDSLPPMYPTFPTRYEP 202
           V+L ++   SL SS + +   P        EISP   PQ   P  +  PM P +     P
Sbjct: 13  VILQSMFLISLCSSQKPEEFLP--------EISPDTSPQPFLPFIAPSPMVP-YINSTMP 72

Query: 203 VLTGRCPVNFSVISNVMDKTASDCSQPMAALVGNVICCPQLSSLLHIFQGFYGLTSDRLV 262
            L+G C +NFS   +++  T+ +C    A L+ NV+CCPQL + L I  G     +  L 
Sbjct: 73  KLSGLCSLNFSASESLIQTTSHNCWTVFAPLLANVMCCPQLDATLTIILGKASKETGLLA 132

Query: 263 LQEAVANDCFSDIISILASRGANQTIPSLCSVKSSNLTGGSCPVTDVATFEKSVNVSKLL 322
           L    +  C SD+  IL  +GA+  +  +CS+ SSNLT  SCPV +V  FE +V+ +KLL
Sbjct: 133 LNRTQSKHCLSDLEQILVGKGASGQLNKICSIHSSNLTSSSCPVINVDEFESTVDTAKLL 192

Query: 323 DACDTVDPLKECCRPICQPAIMEAALKISGRQFSMDTNSNLAGEPNHIDSINDCKRVVYS 382
            AC+ +DP+KECC   CQ AI++AA  IS +     T+++        D INDCK VV  
Sbjct: 193 LACEKIDPVKECCEEACQNAILDAATNISLKASETLTDNS--------DRINDCKNVVNR 252

Query: 383 YISRKLSSDAANTAFRILSACKVNKVCPLDFEQPSEVIKDCRNVAAPNPSCCSSLNTYIA 442
           +++ KL         R L+ CK+N+VCPL F     +  +C N  +    CC ++ +Y++
Sbjct: 253 WLATKLDPSRVKETLRGLANCKINRVCPLVFPHMKHIGGNCSNELSNQTGCCRAMESYVS 312

Query: 443 GIQKQMLITNKQAIICSTMFGSMLRKGGVITNVYELCDVDLKDFSIQGTDTYCIVNLIFL 502
            +QKQ LITN QA+ C+T  G+ L+K  +  N++ +C + LKDFS+Q +           
Sbjct: 313 HLQKQTLITNLQALDCATSLGTKLQKLNITKNIFSVCHISLKDFSLQESG---------- 372

Query: 503 HRRCLLRSLPSDVIFDNSTGFSFTCDLTDNIAAPWPSSSSISTLSLCAPEMSLPALPTTE 562
              CLL SLPSD IFD  TG SFTCDL DNI APWP SSS+S+ S C   + +PALP   
Sbjct: 373 ---CLLPSLPSDAIFDKDTGISFTCDLNDNIPAPWP-SSSLSSASTCKKPVRIPALPAAA 404

Query: 563 TKR 566
           + +
Sbjct: 433 SSQ 404

BLAST of Carg15434 vs. TAIR 10
Match: AT1G61900.2 (unknown protein; FUNCTIONS IN: molecular_function unknown; INVOLVED IN: biological_process unknown; LOCATED IN: anchored to plasma membrane, plasma membrane, anchored to membrane; EXPRESSED IN: 23 plant structures; EXPRESSED DURING: 13 growth stages; BEST Arabidopsis thaliana protein match is: unknown protein (TAIR:AT2G30700.1); Has 35333 Blast hits to 34131 proteins in 2444 species: Archae - 798; Bacteria - 22429; Metazoa - 974; Fungi - 991; Plants - 531; Viruses - 0; Other Eukaryotes - 9610 (source: NCBI BLink). )

HSP 1 Score: 282.0 bits (720), Expect = 1.3e-75
Identity = 162/413 (39.23%), Postives = 229/413 (55.45%), Query Frame = 0

Query: 143 VVLSTLVQHSLVSSTREQAQSPRSVVFDPIEISPAVIPQYPYPNDSLPPMYPTFPTRYEP 202
           V+L ++   SL SS + +   P        EISP   PQ   P  +  PM P +     P
Sbjct: 13  VILQSMFLISLCSSQKPEEFLP--------EISPDTSPQPFLPFIAPSPMVP-YINSTMP 72

Query: 203 VLTGRCPVNFSVISNVMDKTASDCSQPMAALVGNVICCPQLSSLLHIFQGFYGLTSDRLV 262
            L+G C +NFS   +++  T+ +C    A L+ NV+CCPQL + L I  G     +  L 
Sbjct: 73  KLSGLCSLNFSASESLIQTTSHNCWTVFAPLLANVMCCPQLDATLTIILGKASKETGLLA 132

Query: 263 LQEAVANDCFSDIISILASRGANQTIPSLCSVKSSNLTGGSCPVTDVATFEKSVNVSKLL 322
           L    +  C SD+  IL  +GA+  +  +CS+ SSNLT  SCPV +V  FE +V+ +KLL
Sbjct: 133 LNRTQSKHCLSDLEQILVGKGASGQLNKICSIHSSNLTSSSCPVINVDEFESTVDTAKLL 192

Query: 323 DACDTVDPLKECCRPICQPAIMEAALKISGRQFSMDTNSNLAGEPNHIDSINDCKRVVYS 382
            AC+ +DP+KECC   CQ AI++AA  IS +     T+++        D INDCK VV  
Sbjct: 193 LACEKIDPVKECCEEACQNAILDAATNISLKASETLTDNS--------DRINDCKNVVNR 252

Query: 383 YISRKLSSDAANTAFRILSACKVNKVCPLDFEQPSEVIKDCRNVAAPNPSCCSSLNTYIA 442
           +++ KL         R L+ CK+N+VCPL F     +  +C N  +    CC ++ +Y++
Sbjct: 253 WLATKLDPSRVKETLRGLANCKINRVCPLVFPHMKHIGGNCSNELSNQTGCCRAMESYVS 312

Query: 443 GIQKQMLITNKQAIICSTMFGSMLRKGGVITNVYELCDVDLKDFSIQGTDTYCIVNLIFL 502
            +QKQ LITN QA+ C+T  G+ L+K  +  N++ +C + LKDFS+Q  +          
Sbjct: 313 HLQKQTLITNLQALDCATSLGTKLQKLNITKNIFSVCHISLKDFSLQVGNQ--------- 372

Query: 503 HRRCLLRSLPSDVIFDNSTGFSFTCDLTDNIAAPWPSSSSISTLSLCAPEMSL 556
              CLL SLPSD IFD  TG SFTCDL DNI APWP SSS+S+ S C  +  L
Sbjct: 373 ESGCLLPSLPSDAIFDKDTGISFTCDLNDNIPAPWP-SSSLSSASTCKKQFLL 398

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
KAG7029128.10.0e+00100.00putative GPI-anchored protein, partial [Cucurbita argyrosperma subsp. argyrosper... [more]
KAG6597683.11.4e-27393.26putative GPI-anchored protein, partial [Cucurbita argyrosperma subsp. sororia][more]
XP_022932740.11.4e-24992.29uncharacterized GPI-anchored protein At1g61900 [Cucurbita moschata] >XP_02293274... [more]
XP_023540156.16.9e-24992.08uncharacterized GPI-anchored protein At1g61900-like [Cucurbita pepo subsp. pepo]... [more]
XP_022972312.13.8e-24791.46uncharacterized GPI-anchored protein At1g61900-like [Cucurbita maxima] >XP_02297... [more]
Match NameE-valueIdentityDescription
Q8GUI46.6e-7739.01Uncharacterized GPI-anchored protein At1g61900 OS=Arabidopsis thaliana OX=3702 G... [more]
Match NameE-valueIdentityDescription
A0A6J1F2Y96.7e-25092.29uncharacterized GPI-anchored protein At1g61900 OS=Cucurbita moschata OX=3662 GN=... [more]
A0A6J1I4H01.8e-24791.46uncharacterized GPI-anchored protein At1g61900-like OS=Cucurbita maxima OX=3661 ... [more]
A0A6J1IQC31.7e-24085.11uncharacterized GPI-anchored protein At1g61900-like isoform X2 OS=Cucurbita maxi... [more]
A0A6J1GUV61.7e-24085.11uncharacterized GPI-anchored protein At1g61900-like isoform X2 OS=Cucurbita mosc... [more]
A0A6J1IST16.3e-24085.08uncharacterized GPI-anchored protein At1g61900-like isoform X1 OS=Cucurbita maxi... [more]
Match NameE-valueIdentityDescription
AT2G30700.14.5e-17464.11unknown protein; BEST Arabidopsis thaliana protein match is: unknown protein (TA... [more]
AT1G61900.14.7e-7839.01unknown protein; FUNCTIONS IN: molecular_function unknown; INVOLVED IN: biologic... [more]
AT1G61900.34.7e-7839.01unknown protein; BEST Arabidopsis thaliana protein match is: unknown protein (TA... [more]
AT1G61900.21.3e-7539.23unknown protein; FUNCTIONS IN: molecular_function unknown; INVOLVED IN: biologic... [more]
InterPro
Analysis Name: InterPro Annotations of Silver-seed gourd (SMH-JMG-627) v2
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR043891SPARK domainPFAMPF19160SPARKcoord: 205..358
e-value: 5.7E-22
score: 78.8
IPR040336Uncharacterized GPI-anchored protein At1g61900-lkePANTHERPTHR33831GPI-ANCHORED PROTEINcoord: 112..580
NoneNo IPR availablePANTHERPTHR33831:SF4GPI-ANCHORED PROTEINcoord: 112..580

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Carg15434-RACarg15434-RAmRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
cellular_component GO:0016020 membrane