Carg15415 (gene) Silver-seed gourd (SMH-JMG-627) v2

Overview
NameCarg15415
Typegene
OrganismCucurbita argyrosperma subsp. argyrosperma cv. SMH-JMG-627 (Silver-seed gourd (SMH-JMG-627) v2)
Descriptionprotein NRT1/ PTR FAMILY 6.1
LocationCarg_Chr06: 10368182 .. 10370615 (+)
RNA-Seq ExpressionCarg15415
SyntenyCarg15415
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRCDSpolypeptidethree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
CACTATGGACAACAATTCTGTTTTGAGTTCTCTGTGAAGACAGTGGTGGATTTTGTTTTTGTTTCCAGGAATTCACAGGTATGGGAAGTACAGAAATCAAATCACCTCAGGAGATGGTTGAAATGCCTGCAAGATTGGACGAGCAGAGGAAGAAGCTTGGGATGTTTTTCGTTGAATCCGACGATCGGAGGACGGCGTTCGGGCGTGGTTACACTGGAGGAACGACTCCGGTTAATATCCGTGGGAAACCGATTGCTGATCTTTCAAAGACTGGCGGCTGGATAGCAGCCTTCTTCATTTTTGGTAAGCAGCTTGTTGCTTGTTGTGCTTGTTGTGCTTGTTTTGCTTGTTGTTAGTACTTGGAGTTGAAACTTGTGTTGATTTTGTTTACAAGGGAATGAAATGGCAGAGAGAATGGCTTATTTTGGGCTGTCAGTGAACATGGTAGCCTTTATGTTCTATGTGATGCATATGCCATTTACAACTTCATCAGATGCAGTTAACAATTTCCTTGGGATCTCACAAGCATCCTCTGTTCTTGGCGGTTTCATCGCGGATGCATATCTCGGTCGTTATTGGACGATTGCGATCTTTACTACAATCTATCTCGGGGTACGATTCGATCTTACTATAAGCTCCTCCTATGAATTCAAAGCTGATGATGATATTGAAGAAATGGTTGATTTGAACTAAATGCAGGGCTTAATAGGAATAACTCTATGTGCCACAATTAGTGCATTTGTACCAAACCAGGAGAAATGTAATGAGTTATCACTGCTTCTTGGCAAATGTGAGCCAGCCAAGGCATGGCAAATGACTTACCTTTACACAGTCCTTTACTTGACAGGCTTTGGGGCTGCAGGTATTAGGCCATGTGTGTCTTCTTTTGGGGCTGATCAGTTTGATGAGAAAAGCAAAGACTACAAGTCTCATTTGGATCGCTTTTTCAACTTCTTCTACCTTTCTGTCACCACTGGAGCCATTGTGGCCTTCACTGCTGTTGTTTACATTCAAATCAAGCATGGATGGGGAGCTGCTTTTGGCTCCCTAGCCATCGCCATGGGCTTCTCAAACGTCGTCTTCTTTCTCGGTACTCCTTTGTATCGACATCGGTTGCCAGGAGGTAGCCCTCTCACAAGGGTTGCTCAAGTCCTTGTGGCTGCATTCAAGAAGAGAAACGCCTCGTTTTCAAGCAGCGAGATGGTTGGCTTGTACGAGGTCGCTGGCAAGCATTCCGCTATCAAAGGCAGCGGAAAGATCCTTCACACAAATGATTTCAGGTTTGTCGAATTAGAGTAAGAGAGCGTATTAGTATTTTAGGCACGTTAAGTTTTTGAGTTTAGCGGTGATTTACGCAGGTGTTTAGACAAGGCAGCACTCGAGTTAAAGGATGATGGAGGTAATCCAAGCCCATGGAGGCTTTGCACAGTGACTCAAGTGGAGGAAGTGAAGATTCTTTTGAAGCTGATTCCAATACCAGCCTGCACAATCATTCTCAATTTAGTGCTAACAGAATATCTCACTCTTTCAGTGCAACAAGCATACACCTTGAACACTCACATAGGCCGTCTTAAGCTCCCTGTCACATGCATGCCAGTCTTCCCTGGCCTCAGCATATTCCTCATACTTTCTCTCTACTACTCGGTATTCGTTCCGCTCTCTCGGCGCATCACCGGCCACCCGCACGGCGCTTCTCAGCTCCAGAGGGTTGGCATCGGTCTAGCGATCTCGATAATCTCGGTCGCTTGGGCCGGGGTGTTCGAGAGATACCGAAGGAACTACGCTATAAAAAGTGGATATGAGGCCAGTTTTTTGACCCCTATGCCCAACTTGAGTGCCTACTGGCTGCTCATTCAGTATTGTCTCATTGGCATAGCAGAAGTGTTTTGCATTGTTGGTTTGCTTGAGTTCTTATATGAGGAAGCCCCGGACGCCATGAAGAGCATTGGATCGTCCTACGCCGCTCTCGCGGGCGGGTTGGGTTGCTTCGCGGCGTCGTTGTTAAACAGTATTATCAAATCCGTCACGGGGAACCCAAAGGAAGGAACTCCATCTTGGCTTTCTCAAAATATTAACAAGGGACAATTCGATTACTTCTATTGGCTTCTCACAGTCATGAGTGTCATCAACTTCTGCATTTTTCTGTACTCAGCTCATAGGTACAAATACAGGAAAGATCATGAGGTTGGAGAAGGAATCATGGAAAATGGAAGGCATGGTACAATGTAAGATCTGACCCCACTTAGTTGGTTAGTTTTAGAGTAATTTTTAATTGTCATTCCATATAAGAAAATTCGAGTTTTTAAGTTATCAATCTCTCATTTCTCGATCGATTTTTTATCTCATTATTTCAATCTACACGATCAACTAAACATAAAAATATCACTTTATCCGTATATGAATAAATAGTGACACCTAATTATGATGATGTTCT

mRNA sequence

CACTATGGACAACAATTCTGTTTTGAGTTCTCTGTGAAGACAGTGGTGGATTTTGTTTTTGTTTCCAGGAATTCACAGGTATGGGAAGTACAGAAATCAAATCACCTCAGGAGATGGTTGAAATGCCTGCAAGATTGGACGAGCAGAGGAAGAAGCTTGGGATGTTTTTCGTTGAATCCGACGATCGGAGGACGGCGTTCGGGCGTGGTTACACTGGAGGAACGACTCCGGTTAATATCCGTGGGAAACCGATTGCTGATCTTTCAAAGACTGGCGGCTGGATAGCAGCCTTCTTCATTTTTGGGAATGAAATGGCAGAGAGAATGGCTTATTTTGGGCTGTCAGTGAACATGGTAGCCTTTATGTTCTATGTGATGCATATGCCATTTACAACTTCATCAGATGCAGTTAACAATTTCCTTGGGATCTCACAAGCATCCTCTGTTCTTGGCGGTTTCATCGCGGATGCATATCTCGGTCGTTATTGGACGATTGCGATCTTTACTACAATCTATCTCGGGGGCTTAATAGGAATAACTCTATGTGCCACAATTAGTGCATTTGTACCAAACCAGGAGAAATGTAATGAGTTATCACTGCTTCTTGGCAAATGTGAGCCAGCCAAGGCATGGCAAATGACTTACCTTTACACAGTCCTTTACTTGACAGGCTTTGGGGCTGCAGGTATTAGGCCATGTGTGTCTTCTTTTGGGGCTGATCAGTTTGATGAGAAAAGCAAAGACTACAAGTCTCATTTGGATCGCTTTTTCAACTTCTTCTACCTTTCTGTCACCACTGGAGCCATTGTGGCCTTCACTGCTGTTGTTTACATTCAAATCAAGCATGGATGGGGAGCTGCTTTTGGCTCCCTAGCCATCGCCATGGGCTTCTCAAACGTCGTCTTCTTTCTCGGTACTCCTTTGTATCGACATCGGTTGCCAGGAGGTAGCCCTCTCACAAGGGTTGCTCAAGTCCTTGTGGCTGCATTCAAGAAGAGAAACGCCTCGTTTTCAAGCAGCGAGATGGTTGGCTTGTACGAGGTCGCTGGCAAGCATTCCGCTATCAAAGGCAGCGGAAAGATCCTTCACACAAATGATTTCAGGTGTTTAGACAAGGCAGCACTCGAGTTAAAGGATGATGGAGGTAATCCAAGCCCATGGAGGCTTTGCACAGTGACTCAAGTGGAGGAAGTGAAGATTCTTTTGAAGCTGATTCCAATACCAGCCTGCACAATCATTCTCAATTTAGTGCTAACAGAATATCTCACTCTTTCAGTGCAACAAGCATACACCTTGAACACTCACATAGGCCGTCTTAAGCTCCCTGTCACATGCATGCCAGTCTTCCCTGGCCTCAGCATATTCCTCATACTTTCTCTCTACTACTCGGTATTCGTTCCGCTCTCTCGGCGCATCACCGGCCACCCGCACGGCGCTTCTCAGCTCCAGAGGGTTGGCATCGGTCTAGCGATCTCGATAATCTCGGTCGCTTGGGCCGGGGTGTTCGAGAGATACCGAAGGAACTACGCTATAAAAAGTGGATATGAGGCCAGTTTTTTGACCCCTATGCCCAACTTGAGTGCCTACTGGCTGCTCATTCAGTATTGTCTCATTGGCATAGCAGAAGTGTTTTGCATTGTTGGTTTGCTTGAGTTCTTATATGAGGAAGCCCCGGACGCCATGAAGAGCATTGGATCGTCCTACGCCGCTCTCGCGGGCGGGTTGGGTTGCTTCGCGGCGTCGTTGTTAAACAGTATTATCAAATCCGTCACGGGGAACCCAAAGGAAGGAACTCCATCTTGGCTTTCTCAAAATATTAACAAGGGACAATTCGATTACTTCTATTGGCTTCTCACAGTCATGAGTGTCATCAACTTCTGCATTTTTCTGTACTCAGCTCATAGGTACAAATACAGGAAAGATCATGAGGTTGGAGAAGGAATCATGGAAAATGGAAGGCATGGTACAATGTAAGATCTGACCCCACTTAGTTGGTTAGTTTTAGAGTAATTTTTAATTGTCATTCCATATAAGAAAATTCGAGTTTTTAAGTTATCAATCTCTCATTTCTCGATCGATTTTTTATCTCATTATTTCAATCTACACGATCAACTAAACATAAAAATATCACTTTATCCGTATATGAATAAATAGTGACACCTAATTATGATGATGTTCT

Coding sequence (CDS)

ATGGGAAGTACAGAAATCAAATCACCTCAGGAGATGGTTGAAATGCCTGCAAGATTGGACGAGCAGAGGAAGAAGCTTGGGATGTTTTTCGTTGAATCCGACGATCGGAGGACGGCGTTCGGGCGTGGTTACACTGGAGGAACGACTCCGGTTAATATCCGTGGGAAACCGATTGCTGATCTTTCAAAGACTGGCGGCTGGATAGCAGCCTTCTTCATTTTTGGGAATGAAATGGCAGAGAGAATGGCTTATTTTGGGCTGTCAGTGAACATGGTAGCCTTTATGTTCTATGTGATGCATATGCCATTTACAACTTCATCAGATGCAGTTAACAATTTCCTTGGGATCTCACAAGCATCCTCTGTTCTTGGCGGTTTCATCGCGGATGCATATCTCGGTCGTTATTGGACGATTGCGATCTTTACTACAATCTATCTCGGGGGCTTAATAGGAATAACTCTATGTGCCACAATTAGTGCATTTGTACCAAACCAGGAGAAATGTAATGAGTTATCACTGCTTCTTGGCAAATGTGAGCCAGCCAAGGCATGGCAAATGACTTACCTTTACACAGTCCTTTACTTGACAGGCTTTGGGGCTGCAGGTATTAGGCCATGTGTGTCTTCTTTTGGGGCTGATCAGTTTGATGAGAAAAGCAAAGACTACAAGTCTCATTTGGATCGCTTTTTCAACTTCTTCTACCTTTCTGTCACCACTGGAGCCATTGTGGCCTTCACTGCTGTTGTTTACATTCAAATCAAGCATGGATGGGGAGCTGCTTTTGGCTCCCTAGCCATCGCCATGGGCTTCTCAAACGTCGTCTTCTTTCTCGGTACTCCTTTGTATCGACATCGGTTGCCAGGAGGTAGCCCTCTCACAAGGGTTGCTCAAGTCCTTGTGGCTGCATTCAAGAAGAGAAACGCCTCGTTTTCAAGCAGCGAGATGGTTGGCTTGTACGAGGTCGCTGGCAAGCATTCCGCTATCAAAGGCAGCGGAAAGATCCTTCACACAAATGATTTCAGGTGTTTAGACAAGGCAGCACTCGAGTTAAAGGATGATGGAGGTAATCCAAGCCCATGGAGGCTTTGCACAGTGACTCAAGTGGAGGAAGTGAAGATTCTTTTGAAGCTGATTCCAATACCAGCCTGCACAATCATTCTCAATTTAGTGCTAACAGAATATCTCACTCTTTCAGTGCAACAAGCATACACCTTGAACACTCACATAGGCCGTCTTAAGCTCCCTGTCACATGCATGCCAGTCTTCCCTGGCCTCAGCATATTCCTCATACTTTCTCTCTACTACTCGGTATTCGTTCCGCTCTCTCGGCGCATCACCGGCCACCCGCACGGCGCTTCTCAGCTCCAGAGGGTTGGCATCGGTCTAGCGATCTCGATAATCTCGGTCGCTTGGGCCGGGGTGTTCGAGAGATACCGAAGGAACTACGCTATAAAAAGTGGATATGAGGCCAGTTTTTTGACCCCTATGCCCAACTTGAGTGCCTACTGGCTGCTCATTCAGTATTGTCTCATTGGCATAGCAGAAGTGTTTTGCATTGTTGGTTTGCTTGAGTTCTTATATGAGGAAGCCCCGGACGCCATGAAGAGCATTGGATCGTCCTACGCCGCTCTCGCGGGCGGGTTGGGTTGCTTCGCGGCGTCGTTGTTAAACAGTATTATCAAATCCGTCACGGGGAACCCAAAGGAAGGAACTCCATCTTGGCTTTCTCAAAATATTAACAAGGGACAATTCGATTACTTCTATTGGCTTCTCACAGTCATGAGTGTCATCAACTTCTGCATTTTTCTGTACTCAGCTCATAGGTACAAATACAGGAAAGATCATGAGGTTGGAGAAGGAATCATGGAAAATGGAAGGCATGGTACAATGTAA

Protein sequence

MGSTEIKSPQEMVEMPARLDEQRKKLGMFFVESDDRRTAFGRGYTGGTTPVNIRGKPIADLSKTGGWIAAFFIFGNEMAERMAYFGLSVNMVAFMFYVMHMPFTTSSDAVNNFLGISQASSVLGGFIADAYLGRYWTIAIFTTIYLGGLIGITLCATISAFVPNQEKCNELSLLLGKCEPAKAWQMTYLYTVLYLTGFGAAGIRPCVSSFGADQFDEKSKDYKSHLDRFFNFFYLSVTTGAIVAFTAVVYIQIKHGWGAAFGSLAIAMGFSNVVFFLGTPLYRHRLPGGSPLTRVAQVLVAAFKKRNASFSSSEMVGLYEVAGKHSAIKGSGKILHTNDFRCLDKAALELKDDGGNPSPWRLCTVTQVEEVKILLKLIPIPACTIILNLVLTEYLTLSVQQAYTLNTHIGRLKLPVTCMPVFPGLSIFLILSLYYSVFVPLSRRITGHPHGASQLQRVGIGLAISIISVAWAGVFERYRRNYAIKSGYEASFLTPMPNLSAYWLLIQYCLIGIAEVFCIVGLLEFLYEEAPDAMKSIGSSYAALAGGLGCFAASLLNSIIKSVTGNPKEGTPSWLSQNINKGQFDYFYWLLTVMSVINFCIFLYSAHRYKYRKDHEVGEGIMENGRHGTM
Homology
BLAST of Carg15415 vs. NCBI nr
Match: KAG7029147.1 (Protein NRT1/ PTR FAMILY 6.1 [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 1256.5 bits (3250), Expect = 0.0e+00
Identity = 630/630 (100.00%), Postives = 630/630 (100.00%), Query Frame = 0

Query: 1   MGSTEIKSPQEMVEMPARLDEQRKKLGMFFVESDDRRTAFGRGYTGGTTPVNIRGKPIAD 60
           MGSTEIKSPQEMVEMPARLDEQRKKLGMFFVESDDRRTAFGRGYTGGTTPVNIRGKPIAD
Sbjct: 1   MGSTEIKSPQEMVEMPARLDEQRKKLGMFFVESDDRRTAFGRGYTGGTTPVNIRGKPIAD 60

Query: 61  LSKTGGWIAAFFIFGNEMAERMAYFGLSVNMVAFMFYVMHMPFTTSSDAVNNFLGISQAS 120
           LSKTGGWIAAFFIFGNEMAERMAYFGLSVNMVAFMFYVMHMPFTTSSDAVNNFLGISQAS
Sbjct: 61  LSKTGGWIAAFFIFGNEMAERMAYFGLSVNMVAFMFYVMHMPFTTSSDAVNNFLGISQAS 120

Query: 121 SVLGGFIADAYLGRYWTIAIFTTIYLGGLIGITLCATISAFVPNQEKCNELSLLLGKCEP 180
           SVLGGFIADAYLGRYWTIAIFTTIYLGGLIGITLCATISAFVPNQEKCNELSLLLGKCEP
Sbjct: 121 SVLGGFIADAYLGRYWTIAIFTTIYLGGLIGITLCATISAFVPNQEKCNELSLLLGKCEP 180

Query: 181 AKAWQMTYLYTVLYLTGFGAAGIRPCVSSFGADQFDEKSKDYKSHLDRFFNFFYLSVTTG 240
           AKAWQMTYLYTVLYLTGFGAAGIRPCVSSFGADQFDEKSKDYKSHLDRFFNFFYLSVTTG
Sbjct: 181 AKAWQMTYLYTVLYLTGFGAAGIRPCVSSFGADQFDEKSKDYKSHLDRFFNFFYLSVTTG 240

Query: 241 AIVAFTAVVYIQIKHGWGAAFGSLAIAMGFSNVVFFLGTPLYRHRLPGGSPLTRVAQVLV 300
           AIVAFTAVVYIQIKHGWGAAFGSLAIAMGFSNVVFFLGTPLYRHRLPGGSPLTRVAQVLV
Sbjct: 241 AIVAFTAVVYIQIKHGWGAAFGSLAIAMGFSNVVFFLGTPLYRHRLPGGSPLTRVAQVLV 300

Query: 301 AAFKKRNASFSSSEMVGLYEVAGKHSAIKGSGKILHTNDFRCLDKAALELKDDGGNPSPW 360
           AAFKKRNASFSSSEMVGLYEVAGKHSAIKGSGKILHTNDFRCLDKAALELKDDGGNPSPW
Sbjct: 301 AAFKKRNASFSSSEMVGLYEVAGKHSAIKGSGKILHTNDFRCLDKAALELKDDGGNPSPW 360

Query: 361 RLCTVTQVEEVKILLKLIPIPACTIILNLVLTEYLTLSVQQAYTLNTHIGRLKLPVTCMP 420
           RLCTVTQVEEVKILLKLIPIPACTIILNLVLTEYLTLSVQQAYTLNTHIGRLKLPVTCMP
Sbjct: 361 RLCTVTQVEEVKILLKLIPIPACTIILNLVLTEYLTLSVQQAYTLNTHIGRLKLPVTCMP 420

Query: 421 VFPGLSIFLILSLYYSVFVPLSRRITGHPHGASQLQRVGIGLAISIISVAWAGVFERYRR 480
           VFPGLSIFLILSLYYSVFVPLSRRITGHPHGASQLQRVGIGLAISIISVAWAGVFERYRR
Sbjct: 421 VFPGLSIFLILSLYYSVFVPLSRRITGHPHGASQLQRVGIGLAISIISVAWAGVFERYRR 480

Query: 481 NYAIKSGYEASFLTPMPNLSAYWLLIQYCLIGIAEVFCIVGLLEFLYEEAPDAMKSIGSS 540
           NYAIKSGYEASFLTPMPNLSAYWLLIQYCLIGIAEVFCIVGLLEFLYEEAPDAMKSIGSS
Sbjct: 481 NYAIKSGYEASFLTPMPNLSAYWLLIQYCLIGIAEVFCIVGLLEFLYEEAPDAMKSIGSS 540

Query: 541 YAALAGGLGCFAASLLNSIIKSVTGNPKEGTPSWLSQNINKGQFDYFYWLLTVMSVINFC 600
           YAALAGGLGCFAASLLNSIIKSVTGNPKEGTPSWLSQNINKGQFDYFYWLLTVMSVINFC
Sbjct: 541 YAALAGGLGCFAASLLNSIIKSVTGNPKEGTPSWLSQNINKGQFDYFYWLLTVMSVINFC 600

Query: 601 IFLYSAHRYKYRKDHEVGEGIMENGRHGTM 631
           IFLYSAHRYKYRKDHEVGEGIMENGRHGTM
Sbjct: 601 IFLYSAHRYKYRKDHEVGEGIMENGRHGTM 630

BLAST of Carg15415 vs. NCBI nr
Match: XP_022932773.1 (protein NRT1/ PTR FAMILY 6.1 [Cucurbita moschata])

HSP 1 Score: 1248.8 bits (3230), Expect = 0.0e+00
Identity = 626/630 (99.37%), Postives = 628/630 (99.68%), Query Frame = 0

Query: 1   MGSTEIKSPQEMVEMPARLDEQRKKLGMFFVESDDRRTAFGRGYTGGTTPVNIRGKPIAD 60
           MGSTEIKSPQEMVEMPARLDEQRKKLGMFFVESDDRRTAFGRGYTGGTTPVNIRGKPIAD
Sbjct: 1   MGSTEIKSPQEMVEMPARLDEQRKKLGMFFVESDDRRTAFGRGYTGGTTPVNIRGKPIAD 60

Query: 61  LSKTGGWIAAFFIFGNEMAERMAYFGLSVNMVAFMFYVMHMPFTTSSDAVNNFLGISQAS 120
           LSKTGGWIAAFFIFGNEMAERMAYFGLSVNMVAFMFYVMHMPFTTSSDAVNNFLGISQAS
Sbjct: 61  LSKTGGWIAAFFIFGNEMAERMAYFGLSVNMVAFMFYVMHMPFTTSSDAVNNFLGISQAS 120

Query: 121 SVLGGFIADAYLGRYWTIAIFTTIYLGGLIGITLCATISAFVPNQEKCNELSLLLGKCEP 180
           SVLGGFIADAYLGRYWTIAIFTTIYLGGLIGITLCATISAFVPNQE CNELSLLLGKCEP
Sbjct: 121 SVLGGFIADAYLGRYWTIAIFTTIYLGGLIGITLCATISAFVPNQEICNELSLLLGKCEP 180

Query: 181 AKAWQMTYLYTVLYLTGFGAAGIRPCVSSFGADQFDEKSKDYKSHLDRFFNFFYLSVTTG 240
           AKAWQMTYLYTVLYLTGFGAAGIRPCVSSFGADQFDEKSKDYKSHLDRFFNFFYLSVTTG
Sbjct: 181 AKAWQMTYLYTVLYLTGFGAAGIRPCVSSFGADQFDEKSKDYKSHLDRFFNFFYLSVTTG 240

Query: 241 AIVAFTAVVYIQIKHGWGAAFGSLAIAMGFSNVVFFLGTPLYRHRLPGGSPLTRVAQVLV 300
           AIVAFTAVVYIQIKHGWGAAFGSLAIAMGFSNVVFFLGTPLYRHRLPGGSPLTRVAQVLV
Sbjct: 241 AIVAFTAVVYIQIKHGWGAAFGSLAIAMGFSNVVFFLGTPLYRHRLPGGSPLTRVAQVLV 300

Query: 301 AAFKKRNASFSSSEMVGLYEVAGKHSAIKGSGKILHTNDFRCLDKAALELKDDGGNPSPW 360
           AAFKKRNASFSSSEMVGLYEVAGKHSAIKGSGKILHTNDFRCLDKAALELKDDGGNPSPW
Sbjct: 301 AAFKKRNASFSSSEMVGLYEVAGKHSAIKGSGKILHTNDFRCLDKAALELKDDGGNPSPW 360

Query: 361 RLCTVTQVEEVKILLKLIPIPACTIILNLVLTEYLTLSVQQAYTLNTHIGRLKLPVTCMP 420
           RLCTVTQVEEVKILLKLIPIPACTIILNLVLTEYLTLSVQQAYTLNTHIGRLKLPVTCMP
Sbjct: 361 RLCTVTQVEEVKILLKLIPIPACTIILNLVLTEYLTLSVQQAYTLNTHIGRLKLPVTCMP 420

Query: 421 VFPGLSIFLILSLYYSVFVPLSRRITGHPHGASQLQRVGIGLAISIISVAWAGVFERYRR 480
           VFPGLSIFLILSLYYSVFVPLSRRITGHPHGASQLQRVGIGLAISIISVAWAGVFERYRR
Sbjct: 421 VFPGLSIFLILSLYYSVFVPLSRRITGHPHGASQLQRVGIGLAISIISVAWAGVFERYRR 480

Query: 481 NYAIKSGYEASFLTPMPNLSAYWLLIQYCLIGIAEVFCIVGLLEFLYEEAPDAMKSIGSS 540
           NYAIK+GYEASFLTPMPNLSAYWLLIQYCLIGIAEVFCIVGLLEFLYEEAPDAMKSIGSS
Sbjct: 481 NYAIKNGYEASFLTPMPNLSAYWLLIQYCLIGIAEVFCIVGLLEFLYEEAPDAMKSIGSS 540

Query: 541 YAALAGGLGCFAASLLNSIIKSVTGNPKEGTPSWLSQNINKGQFDYFYWLLTVMSVINFC 600
           YAALAGGLGCFAASLLNSIIKSVTGN KEGTPSWLSQNINKGQFDYFYWLLTVMS+INFC
Sbjct: 541 YAALAGGLGCFAASLLNSIIKSVTGNTKEGTPSWLSQNINKGQFDYFYWLLTVMSIINFC 600

Query: 601 IFLYSAHRYKYRKDHEVGEGIMENGRHGTM 631
           IFLYSAHRYKYRKDHEVGEGIMENGRHGTM
Sbjct: 601 IFLYSAHRYKYRKDHEVGEGIMENGRHGTM 630

BLAST of Carg15415 vs. NCBI nr
Match: XP_022972287.1 (protein NRT1/ PTR FAMILY 6.1 [Cucurbita maxima])

HSP 1 Score: 1243.4 bits (3216), Expect = 0.0e+00
Identity = 622/630 (98.73%), Postives = 627/630 (99.52%), Query Frame = 0

Query: 1   MGSTEIKSPQEMVEMPARLDEQRKKLGMFFVESDDRRTAFGRGYTGGTTPVNIRGKPIAD 60
           MGSTEIKSPQEMVEMPARLDEQRKKLGMFFVESDDRRTAFGRGYTGGTTPVNIRGKPIAD
Sbjct: 1   MGSTEIKSPQEMVEMPARLDEQRKKLGMFFVESDDRRTAFGRGYTGGTTPVNIRGKPIAD 60

Query: 61  LSKTGGWIAAFFIFGNEMAERMAYFGLSVNMVAFMFYVMHMPFTTSSDAVNNFLGISQAS 120
           LSKTGGW AAFFIFGNEMAERMAYFGLSVNMVAFMFYVMHMPFTTSSDAVNNFLGISQAS
Sbjct: 61  LSKTGGWTAAFFIFGNEMAERMAYFGLSVNMVAFMFYVMHMPFTTSSDAVNNFLGISQAS 120

Query: 121 SVLGGFIADAYLGRYWTIAIFTTIYLGGLIGITLCATISAFVPNQEKCNELSLLLGKCEP 180
           SVLGGFIADAYLGRYWTIAIFTTIYLGGL GITLCATISAFVPNQ+ C+ELSLLLGKCEP
Sbjct: 121 SVLGGFIADAYLGRYWTIAIFTTIYLGGLTGITLCATISAFVPNQKICDELSLLLGKCEP 180

Query: 181 AKAWQMTYLYTVLYLTGFGAAGIRPCVSSFGADQFDEKSKDYKSHLDRFFNFFYLSVTTG 240
           AKAWQMTYLYTVLYLTGFGAAGIRPCVSSFGADQFDEKSKDYKSHLDRFFNFFYLSVTTG
Sbjct: 181 AKAWQMTYLYTVLYLTGFGAAGIRPCVSSFGADQFDEKSKDYKSHLDRFFNFFYLSVTTG 240

Query: 241 AIVAFTAVVYIQIKHGWGAAFGSLAIAMGFSNVVFFLGTPLYRHRLPGGSPLTRVAQVLV 300
           AIVAFTAVVYIQIKHGWGAAFGSLAIAMGFSNVVFFLGTPLYRHRLPGGSPLTRVAQVLV
Sbjct: 241 AIVAFTAVVYIQIKHGWGAAFGSLAIAMGFSNVVFFLGTPLYRHRLPGGSPLTRVAQVLV 300

Query: 301 AAFKKRNASFSSSEMVGLYEVAGKHSAIKGSGKILHTNDFRCLDKAALELKDDGGNPSPW 360
           AAF+KRNASFSSSEMVGLYEVAGKHSAIKGSGKILHTNDFRCLDKAALELKDDGGNPSPW
Sbjct: 301 AAFRKRNASFSSSEMVGLYEVAGKHSAIKGSGKILHTNDFRCLDKAALELKDDGGNPSPW 360

Query: 361 RLCTVTQVEEVKILLKLIPIPACTIILNLVLTEYLTLSVQQAYTLNTHIGRLKLPVTCMP 420
           RLCTVTQVEEVKILLKLIPIPACTIILNLVLTEYLTLSVQQAYTLNTH+GRLKLPVTCMP
Sbjct: 361 RLCTVTQVEEVKILLKLIPIPACTIILNLVLTEYLTLSVQQAYTLNTHMGRLKLPVTCMP 420

Query: 421 VFPGLSIFLILSLYYSVFVPLSRRITGHPHGASQLQRVGIGLAISIISVAWAGVFERYRR 480
           VFPGLSIFLILSLYYSVFVPLSRRITGHPHGASQLQRVGIGLAISIISVAWAGVFERYRR
Sbjct: 421 VFPGLSIFLILSLYYSVFVPLSRRITGHPHGASQLQRVGIGLAISIISVAWAGVFERYRR 480

Query: 481 NYAIKSGYEASFLTPMPNLSAYWLLIQYCLIGIAEVFCIVGLLEFLYEEAPDAMKSIGSS 540
           NYAIKSGYEASFLTPMPNLSAYWLLIQYCLIGIAEVFCIVGLLEFLYEEAPDAMKSIGSS
Sbjct: 481 NYAIKSGYEASFLTPMPNLSAYWLLIQYCLIGIAEVFCIVGLLEFLYEEAPDAMKSIGSS 540

Query: 541 YAALAGGLGCFAASLLNSIIKSVTGNPKEGTPSWLSQNINKGQFDYFYWLLTVMSVINFC 600
           YAALAGGLGCFAASLLNSIIKSVTGNPKEGTPSWLSQNINKGQFDYFYWLLTVMS+INFC
Sbjct: 541 YAALAGGLGCFAASLLNSIIKSVTGNPKEGTPSWLSQNINKGQFDYFYWLLTVMSIINFC 600

Query: 601 IFLYSAHRYKYRKDHEVGEGIMENGRHGTM 631
           IFLYSAHRYKYRKDHEVGEGIMENGRHGTM
Sbjct: 601 IFLYSAHRYKYRKDHEVGEGIMENGRHGTM 630

BLAST of Carg15415 vs. NCBI nr
Match: XP_023538659.1 (protein NRT1/ PTR FAMILY 6.1 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 1242.6 bits (3214), Expect = 0.0e+00
Identity = 622/630 (98.73%), Postives = 626/630 (99.37%), Query Frame = 0

Query: 1   MGSTEIKSPQEMVEMPARLDEQRKKLGMFFVESDDRRTAFGRGYTGGTTPVNIRGKPIAD 60
           MGSTEIKSPQEMVEMPARLDEQRKKLGMFFVESDDRRTAFGRGYTGGTTPVNIRGKPIAD
Sbjct: 1   MGSTEIKSPQEMVEMPARLDEQRKKLGMFFVESDDRRTAFGRGYTGGTTPVNIRGKPIAD 60

Query: 61  LSKTGGWIAAFFIFGNEMAERMAYFGLSVNMVAFMFYVMHMPFTTSSDAVNNFLGISQAS 120
           LSKTGGWIAAFFIFGNEMAERMAYFGLSVNMVAFMFYVMHMPFTTSSDAVNNFLGISQAS
Sbjct: 61  LSKTGGWIAAFFIFGNEMAERMAYFGLSVNMVAFMFYVMHMPFTTSSDAVNNFLGISQAS 120

Query: 121 SVLGGFIADAYLGRYWTIAIFTTIYLGGLIGITLCATISAFVPNQEKCNELSLLLGKCEP 180
           SVLGGFIADAYLGRYWTIAIFTTIYLGGLIGITLCATISAFVPNQE C+ELSLLLGKCEP
Sbjct: 121 SVLGGFIADAYLGRYWTIAIFTTIYLGGLIGITLCATISAFVPNQEICDELSLLLGKCEP 180

Query: 181 AKAWQMTYLYTVLYLTGFGAAGIRPCVSSFGADQFDEKSKDYKSHLDRFFNFFYLSVTTG 240
           AKAWQMTYLYTVLYLTGFGAAGIRPCVSSFGADQFDEKSKDYKSHLDRFFNFFYLSVTTG
Sbjct: 181 AKAWQMTYLYTVLYLTGFGAAGIRPCVSSFGADQFDEKSKDYKSHLDRFFNFFYLSVTTG 240

Query: 241 AIVAFTAVVYIQIKHGWGAAFGSLAIAMGFSNVVFFLGTPLYRHRLPGGSPLTRVAQVLV 300
           AIVAFTAVVYIQIKHGWGAAFGSLAIAMGFSN VFFLGTPLYRHRLPGGSPLTRVAQVLV
Sbjct: 241 AIVAFTAVVYIQIKHGWGAAFGSLAIAMGFSNAVFFLGTPLYRHRLPGGSPLTRVAQVLV 300

Query: 301 AAFKKRNASFSSSEMVGLYEVAGKHSAIKGSGKILHTNDFRCLDKAALELKDDGGNPSPW 360
           AAF+KRNA FSSSEMVGLYEV GKHSAIKGSGKILHTNDFRCLDKAALELKDDGGNPSPW
Sbjct: 301 AAFRKRNALFSSSEMVGLYEVDGKHSAIKGSGKILHTNDFRCLDKAALELKDDGGNPSPW 360

Query: 361 RLCTVTQVEEVKILLKLIPIPACTIILNLVLTEYLTLSVQQAYTLNTHIGRLKLPVTCMP 420
           RLCTVTQVEEVKILLKLIPIPACTIILNLVLTEYLTLSVQQAYTLNTHIGRLKLPVTCMP
Sbjct: 361 RLCTVTQVEEVKILLKLIPIPACTIILNLVLTEYLTLSVQQAYTLNTHIGRLKLPVTCMP 420

Query: 421 VFPGLSIFLILSLYYSVFVPLSRRITGHPHGASQLQRVGIGLAISIISVAWAGVFERYRR 480
           VFPGLSIFLILSLYYSVFVPLSRRITGHPHGASQLQRVGIGLAISIISVAWAGVFERYRR
Sbjct: 421 VFPGLSIFLILSLYYSVFVPLSRRITGHPHGASQLQRVGIGLAISIISVAWAGVFERYRR 480

Query: 481 NYAIKSGYEASFLTPMPNLSAYWLLIQYCLIGIAEVFCIVGLLEFLYEEAPDAMKSIGSS 540
           NYAIK+GYEASFLTPMPNLSAYWLLIQYCLIGIAEVFCIVGLLEFLYEEAPDAMKSIGSS
Sbjct: 481 NYAIKNGYEASFLTPMPNLSAYWLLIQYCLIGIAEVFCIVGLLEFLYEEAPDAMKSIGSS 540

Query: 541 YAALAGGLGCFAASLLNSIIKSVTGNPKEGTPSWLSQNINKGQFDYFYWLLTVMSVINFC 600
           YAALAGGLGCFAASLLNSIIKSVTGNPKEGTPSWLSQNINKGQFDYFYWLLTVMS+INFC
Sbjct: 541 YAALAGGLGCFAASLLNSIIKSVTGNPKEGTPSWLSQNINKGQFDYFYWLLTVMSIINFC 600

Query: 601 IFLYSAHRYKYRKDHEVGEGIMENGRHGTM 631
           IFLYSAHRYKYRKDHEVGEGIMENGRHGTM
Sbjct: 601 IFLYSAHRYKYRKDHEVGEGIMENGRHGTM 630

BLAST of Carg15415 vs. NCBI nr
Match: KAG6597701.1 (Protein NRT1/ PTR FAMILY 6.1, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 1180.6 bits (3053), Expect = 0.0e+00
Identity = 601/630 (95.40%), Postives = 601/630 (95.40%), Query Frame = 0

Query: 1   MGSTEIKSPQEMVEMPARLDEQRKKLGMFFVESDDRRTAFGRGYTGGTTPVNIRGKPIAD 60
           MGSTEIKSPQEMVEMPARLDEQRKKLGMFFVESDDRRTAFGRGYTGGTTPVNIRGKPIAD
Sbjct: 1   MGSTEIKSPQEMVEMPARLDEQRKKLGMFFVESDDRRTAFGRGYTGGTTPVNIRGKPIAD 60

Query: 61  LSKTGGWIAAFFIFGNEMAERMAYFGLSVNMVAFMFYVMHMPFTTSSDAVNNFLGISQAS 120
           LSKTGGWIAAFFIFGNEMAERMAYFGLSVNMVAFMFYVMHMPFTTSSDAVNNFLGISQAS
Sbjct: 61  LSKTGGWIAAFFIFGNEMAERMAYFGLSVNMVAFMFYVMHMPFTTSSDAVNNFLGISQAS 120

Query: 121 SVLGGFIADAYLGRYWTIAIFTTIYLGGLIGITLCATISAFVPNQEKCNELSLLLGKCEP 180
           SVLGGFIADAYLGRYWTIAIFTTIYLGGLIGITLCATISAFVPNQEKC            
Sbjct: 121 SVLGGFIADAYLGRYWTIAIFTTIYLGGLIGITLCATISAFVPNQEKC------------ 180

Query: 181 AKAWQMTYLYTVLYLTGFGAAGIRPCVSSFGADQFDEKSKDYKSHLDRFFNFFYLSVTTG 240
                            FGAAGIRPCVSSFGADQFDEKSKDYKSHLDRFFNFFYLSVTTG
Sbjct: 181 -----------------FGAAGIRPCVSSFGADQFDEKSKDYKSHLDRFFNFFYLSVTTG 240

Query: 241 AIVAFTAVVYIQIKHGWGAAFGSLAIAMGFSNVVFFLGTPLYRHRLPGGSPLTRVAQVLV 300
           AIVAFTAVVYIQIKHGWGAAFGSLAIAMGFSNVVFFLGTPLYRHRLPGGSPLTRVAQVLV
Sbjct: 241 AIVAFTAVVYIQIKHGWGAAFGSLAIAMGFSNVVFFLGTPLYRHRLPGGSPLTRVAQVLV 300

Query: 301 AAFKKRNASFSSSEMVGLYEVAGKHSAIKGSGKILHTNDFRCLDKAALELKDDGGNPSPW 360
           AAFKKRNASFSSSEMVGLYEVAGKHSAIKGSGKILHTNDFRCLDKAALELKDDGGNPSPW
Sbjct: 301 AAFKKRNASFSSSEMVGLYEVAGKHSAIKGSGKILHTNDFRCLDKAALELKDDGGNPSPW 360

Query: 361 RLCTVTQVEEVKILLKLIPIPACTIILNLVLTEYLTLSVQQAYTLNTHIGRLKLPVTCMP 420
           RLCTVTQVEEVKILLKLIPIPACTIILNLVLTEYLTLSVQQAYTLNTHIGRLKLPVTCMP
Sbjct: 361 RLCTVTQVEEVKILLKLIPIPACTIILNLVLTEYLTLSVQQAYTLNTHIGRLKLPVTCMP 420

Query: 421 VFPGLSIFLILSLYYSVFVPLSRRITGHPHGASQLQRVGIGLAISIISVAWAGVFERYRR 480
           VFPGLSIFLILSLYYSVFVPLSRRITGHPHGASQLQRVGIGLAISIISVAWAGVFERYRR
Sbjct: 421 VFPGLSIFLILSLYYSVFVPLSRRITGHPHGASQLQRVGIGLAISIISVAWAGVFERYRR 480

Query: 481 NYAIKSGYEASFLTPMPNLSAYWLLIQYCLIGIAEVFCIVGLLEFLYEEAPDAMKSIGSS 540
           NYAIKSGYEASFLTPMPNLSAYWLLIQYCLIGIAEVFCIVGLLEFLYEEAPDAMKSIGSS
Sbjct: 481 NYAIKSGYEASFLTPMPNLSAYWLLIQYCLIGIAEVFCIVGLLEFLYEEAPDAMKSIGSS 540

Query: 541 YAALAGGLGCFAASLLNSIIKSVTGNPKEGTPSWLSQNINKGQFDYFYWLLTVMSVINFC 600
           YAALAGGLGCFAASLLNSIIKSVTGNPKEGTPSWLSQNINKGQFDYFYWLLTVMSVINFC
Sbjct: 541 YAALAGGLGCFAASLLNSIIKSVTGNPKEGTPSWLSQNINKGQFDYFYWLLTVMSVINFC 600

Query: 601 IFLYSAHRYKYRKDHEVGEGIMENGRHGTM 631
           IFLYSAHRYKYRKDHEVGEGIMENGRHGTM
Sbjct: 601 IFLYSAHRYKYRKDHEVGEGIMENGRHGTM 601

BLAST of Carg15415 vs. ExPASy Swiss-Prot
Match: Q9LYR6 (Protein NRT1/ PTR FAMILY 6.1 OS=Arabidopsis thaliana OX=3702 GN=NPF6.1 PE=2 SV=1)

HSP 1 Score: 977.6 bits (2526), Expect = 6.5e-284
Identity = 487/613 (79.45%), Postives = 549/613 (89.56%), Query Frame = 0

Query: 1   MGSTEIKSPQEMVEMPARLD-EQRKKLGMFFVESDDRRTAFGRGYTGGTTPVNIRGKPIA 60
           M ++EIKSP  + E P       RK+L +FF+ESD+RR A GRGYTGGTTPVNI GKPIA
Sbjct: 1   MVASEIKSPVSVPETPGSSSVHHRKQLSVFFIESDNRRLALGRGYTGGTTPVNIHGKPIA 60

Query: 61  DLSKTGGWIAAFFIFGNEMAERMAYFGLSVNMVAFMFYVMHMPFTTSSDAVNNFLGISQA 120
           +LSKTGGWIAAFFIFGNEMAERMAYFGLSVNMVAFMFYVMH PF +SS+AVNNFLGISQA
Sbjct: 61  NLSKTGGWIAAFFIFGNEMAERMAYFGLSVNMVAFMFYVMHRPFESSSNAVNNFLGISQA 120

Query: 121 SSVLGGFIADAYLGRYWTIAIFTTIYLGGLIGITLCATISAFVPNQEKCNELSLLLGKCE 180
           SSVLGGF+ADAYLGRYWTIAIFTT+YL GLIGITL A++  FVP+Q  C +LSLLLG CE
Sbjct: 121 SSVLGGFLADAYLGRYWTIAIFTTMYLVGLIGITLGASLKMFVPDQSNCGQLSLLLGNCE 180

Query: 181 PAKAWQMTYLYTVLYLTGFGAAGIRPCVSSFGADQFDEKSKDYKSHLDRFFNFFYLSVTT 240
            AK+WQM YLYTVLY+TGFGAAGIRPCVSSFGADQFDEKSKDYK+HLDRFFNFFYLSVT 
Sbjct: 181 EAKSWQMLYLYTVLYITGFGAAGIRPCVSSFGADQFDEKSKDYKTHLDRFFNFFYLSVTL 240

Query: 241 GAIVAFTAVVYIQIKHGWGAAFGSLAIAMGFSNVVFFLGTPLYRHRLPGGSPLTRVAQVL 300
           GAI+AFT VVY+Q++ GWG AFG+LA+AMG SN +FF GTPLYRHRLPGGSPLTRVAQVL
Sbjct: 241 GAIIAFTLVVYVQMELGWGMAFGTLAVAMGISNALFFAGTPLYRHRLPGGSPLTRVAQVL 300

Query: 301 VAAFKKRNASFSSSEMVGLYEVAGKHSAIKGSGKILHTNDFRCLDKAALELKDDGGNPSP 360
           VAAF+KRNA+F+SSE +GLYEV G  SAI GS KI H+NDF  LDKAALELK+DG  PSP
Sbjct: 301 VAAFRKRNAAFTSSEFIGLYEVPGLKSAINGSRKIPHSNDFIWLDKAALELKEDGLEPSP 360

Query: 361 WRLCTVTQVEEVKILLKLIPIPACTIILNLVLTEYLTLSVQQAYTLNTHIGRLKLPVTCM 420
           W+LCTVTQVEEVKIL++LIPIP CTI+L+LVLTEYLTLSVQQAYTLNTHI  LKLPVTCM
Sbjct: 361 WKLCTVTQVEEVKILIRLIPIPTCTIMLSLVLTEYLTLSVQQAYTLNTHIQHLKLPVTCM 420

Query: 421 PVFPGLSIFLILSLYYSVFVPLSRRITGHPHGASQLQRVGIGLAISIISVAWAGVFERYR 480
           PVFPGLSIFLILSLYYSVFVP++RRITG+PHGASQLQRVGIGLA+SIISVAWAG+FE YR
Sbjct: 421 PVFPGLSIFLILSLYYSVFVPITRRITGNPHGASQLQRVGIGLAVSIISVAWAGLFENYR 480

Query: 481 RNYAIKSGYEASFLTPMPNLSAYWLLIQYCLIGIAEVFCIVGLLEFLYEEAPDAMKSIGS 540
           R+YAI++G+E +FLT MP+L+AYWLLIQYCLIGIAEVFCIVGLLEFLYEEAPDAMKSIGS
Sbjct: 481 RHYAIQNGFEFNFLTQMPDLTAYWLLIQYCLIGIAEVFCIVGLLEFLYEEAPDAMKSIGS 540

Query: 541 SYAALAGGLGCFAASLLNSIIKSVTGNPKEGTPSWLSQNINKGQFDYFYWLLTVMSVINF 600
           +YAALAGGLGCFAA++LN+I+K+ T +      SWLSQNIN G+FD  YWLLT++S +NF
Sbjct: 541 AYAALAGGLGCFAATILNNIVKAATRD--SDGKSWLSQNINTGRFDCLYWLLTLLSFLNF 600

Query: 601 CIFLYSAHRYKYR 613
           C+FL+SAHRYKYR
Sbjct: 601 CVFLWSAHRYKYR 611

BLAST of Carg15415 vs. ExPASy Swiss-Prot
Match: Q9M390 (Protein NRT1/ PTR FAMILY 8.1 OS=Arabidopsis thaliana OX=3702 GN=NPF8.1 PE=1 SV=1)

HSP 1 Score: 416.8 bits (1070), Expect = 4.4e-115
Identity = 227/568 (39.96%), Postives = 346/568 (60.92%), Query Frame = 0

Query: 51  VNIRGKPIADLSKTGGWIAAFFIFGNEMAERMAYFGLSVNMVAFMFYVMHMPFTTSSDAV 110
           V+I   P A+  KTG W A  FI GNE  ER+AY+G+  N+V ++   ++    T+++ V
Sbjct: 13  VDIHKNP-ANKEKTGNWKACRFILGNECCERLAYYGMGTNLVNYLESRLNQGNATAANNV 72

Query: 111 NNFLGISQASSVLGGFIADAYLGRYWTIAIFTTIYLGGLIGITLCATISAFVPNQEKCNE 170
            N+ G    + ++G FIADAYLGRYWTIA F  IY+ G+  +TL A++    P    CN 
Sbjct: 73  TNWSGTCYITPLIGAFIADAYLGRYWTIATFVFIYVSGMTLLTLSASVPGLKPG--NCN- 132

Query: 171 LSLLLGKCEPAKAWQMTYLYTVLYLTGFGAAGIRPCVSSFGADQFDEKSKDYKSHLDRFF 230
                  C P  + Q    +  LY+   G  GI+PCVSSFGADQFDE  ++ K     FF
Sbjct: 133 ----ADTCHPNSS-QTAVFFVALYMIALGTGGIKPCVSSFGADQFDENDENEKIKKSSFF 192

Query: 231 NFFYLSVTTGAIVAFTAVVYIQIKHGWGAAFGSLAIAMGFSNVVFFLGTPLYRHRLPGGS 290
           N+FY S+  GA++A T +V+IQ+  GWG  FG   +AM  +   FF G+  YR + PGGS
Sbjct: 193 NWFYFSINVGALIAATVLVWIQMNVGWGWGFGVPTVAMVIAVCFFFFGSRFYRLQRPGGS 252

Query: 291 PLTRVAQVLVAAFKKRNASFSSSEMVGLYEVAGKHSAIKGSGKILHTNDFRCLDKAALEL 350
           PLTR+ QV+VAAF+K +      + + L+E A   S IKGS K++HT++ +  DKAA+E 
Sbjct: 253 PLTRIFQVIVAAFRKISVKVPEDKSL-LFETADDESNIKGSRKLVHTDNLKFFDKAAVES 312

Query: 351 KDDG---GNPSPWRLCTVTQVEEVKILLKLIPIPACTIILNLVLTEYLTLSVQQAYTLNT 410
           + D    G  +PWRLC+VTQVEE+K ++ L+P+ A  I+   V ++  T+ V Q  T++ 
Sbjct: 313 QSDSIKDGEVNPWRLCSVTQVEELKSIITLLPVWATGIVFATVYSQMSTMFVLQGNTMDQ 372

Query: 411 HIGR-LKLPVTCMPVFPGLSIFLILSLYYSVFVPLSRRITGHPHGASQLQRVGIGLAISI 470
           H+G+  ++P   + +F  +S+     +Y    +PL+R+ T +  G +QLQR+GIGL +SI
Sbjct: 373 HMGKNFEIPSASLSLFDTVSVLFWTPVYDQFIIPLARKFTRNERGFTQLQRMGIGLVVSI 432

Query: 471 ISVAWAGVFERYRRNYA-IKSGYEASFLTPMPNLSAYWLLIQYCLIGIAEVFCIVGLLEF 530
            ++  AGV E  R +Y    + Y+   +    ++S +W + QY LIG AEVF  +G LEF
Sbjct: 433 FAMITAGVLEVVRLDYVKTHNAYDQKQI----HMSIFWQIPQYLLIGCAEVFTFIGQLEF 492

Query: 531 LYEEAPDAMKSIGSSYAALAGGLGCFAASLLNSIIKSVTGNPKEGTPSWLSQNINKGQFD 590
            Y++APDAM+S+ S+ +     LG + +++L +++  +T   K G P W+  N+N+G  D
Sbjct: 493 FYDQAPDAMRSLCSALSLTTVALGNYLSTVLVTVVMKIT--KKNGKPGWIPDNLNRGHLD 552

Query: 591 YFYWLLTVMSVINFCIFLYSAHRYKYRK 614
           YF++LL  +S +NF ++L+ + RYKY+K
Sbjct: 553 YFFYLLATLSFLNFLVYLWISKRYKYKK 564

BLAST of Carg15415 vs. ExPASy Swiss-Prot
Match: Q9LFB8 (Protein NRT1/ PTR FAMILY 8.2 OS=Arabidopsis thaliana OX=3702 GN=NPF8.2 PE=2 SV=1)

HSP 1 Score: 408.3 bits (1048), Expect = 1.6e-112
Identity = 231/567 (40.74%), Postives = 336/567 (59.26%), Query Frame = 0

Query: 51  VNIRGKPIADLSKTGGWIAAFFIFGNEMAERMAYFGLSVNMVAFMFYVMHMPFTTSSDAV 110
           ++I  KP A+ +KTG W A  FI G E  ER+AY+G+S N++ ++   M+M   ++S +V
Sbjct: 14  LDIHKKP-ANKNKTGTWKACRFILGTECCERLAYYGMSTNLINYLEKQMNMENVSASKSV 73

Query: 111 NNFLGISQASSVLGGFIADAYLGRYWTIAIFTTIYLGGLIGITLCATISAFVPNQEKCNE 170
           +N+ G   A+ ++G FIADAYLGRYWTIA F  IY+ G+  +T+ A++    P    C+ 
Sbjct: 74  SNWSGTCYATPLIGAFIADAYLGRYWTIASFVVIYIAGMTLLTISASVPGLTPT---CS- 133

Query: 171 LSLLLGKCEPAKAWQMTYLYTVLYLTGFGAAGIRPCVSSFGADQFDEKSKDYKSHLDRFF 230
                G+   A A Q    +  LYL   G  GI+PCVSSFGADQFD+  +  K     FF
Sbjct: 134 -----GETCHATAGQTAITFIALYLIALGTGGIKPCVSSFGADQFDDTDEKEKESKSSFF 193

Query: 231 NFFYLSVTTGAIVAFTAVVYIQIKHGWGAAFGSLAIAMGFSNVVFFLGTPLYRHRLPGGS 290
           N+FY  +  GA++A + +V+IQ+  GWG   G   +AM  + V FF G+  YR + PGGS
Sbjct: 194 NWFYFVINVGAMIASSVLVWIQMNVGWGWGLGVPTVAMAIAVVFFFAGSNFYRLQKPGGS 253

Query: 291 PLTRVAQVLVAAFKKRNASFSSSEMVGLYEVAGKHSAIKGSGKILHTNDFRCLDKAALEL 350
           PLTR+ QV+VA+ +K        E + LYE     S+I GS K+ HT      DKAA+E 
Sbjct: 254 PLTRMLQVIVASCRKSKVKIPEDESL-LYENQDAESSIIGSRKLEHTKILTFFDKAAVET 313

Query: 351 KDD---GGNPSPWRLCTVTQVEEVKILLKLIPIPACTIILNLVLTEYLTLSVQQAYTLNT 410
           + D       S W+LCTVTQVEE+K L++L+PI A  I+   V ++  T+ V Q  TL+ 
Sbjct: 314 ESDNKGAAKSSSWKLCTVTQVEELKALIRLLPIWATGIVFASVYSQMGTVFVLQGNTLDQ 373

Query: 411 HIG-RLKLPVTCMPVFPGLSIFLILSLYYSVFVPLSRRITGHPHGASQLQRVGIGLAISI 470
           H+G   K+P   + +F  LS+     +Y  + VP +R+ TGH  G +QLQR+GIGL ISI
Sbjct: 374 HMGPNFKIPSASLSLFDTLSVLFWAPVYDKLIVPFARKYTGHERGFTQLQRIGIGLVISI 433

Query: 471 ISVAWAGVFERYRRNYAIKSGYEASFLTPMPNLSAYWLLIQYCLIGIAEVFCIVGLLEFL 530
            S+  AG+ E  R NY            PM   + +W + QY L+G AEVF  +G LEF 
Sbjct: 434 FSMVSAGILEVARLNYVQTHNLYNEETIPM---TIFWQVPQYFLVGCAEVFTFIGQLEFF 493

Query: 531 YEEAPDAMKSIGSSYAALAGGLGCFAASLLNSIIKSVTGNPKEGTPSWLSQNINKGQFDY 590
           Y++APDAM+S+ S+ +  A   G + ++ L +++  VT +   G P W+++N+N G  DY
Sbjct: 494 YDQAPDAMRSLCSALSLTAIAFGNYLSTFLVTLVTKVTRS--GGRPGWIAKNLNNGHLDY 553

Query: 591 FYWLLTVMSVINFCIFLYSAHRYKYRK 614
           F+WLL  +S +NF ++L+ A  Y Y+K
Sbjct: 554 FFWLLAGLSFLNFLVYLWIAKWYTYKK 564

BLAST of Carg15415 vs. ExPASy Swiss-Prot
Match: P46032 (Protein NRT1/ PTR FAMILY 8.3 OS=Arabidopsis thaliana OX=3702 GN=NPF8.3 PE=1 SV=1)

HSP 1 Score: 407.9 bits (1047), Expect = 2.1e-112
Identity = 228/566 (40.28%), Postives = 334/566 (59.01%), Query Frame = 0

Query: 51  VNIRGKPIADLSKTGGWIAAFFIFGNEMAERMAYFGLSVNMVAFMFYVMHMPFTTSSDAV 110
           V+  G P     KTG W A  FI GNE  ER+AY+G++ N++ ++   +H    +++  V
Sbjct: 30  VDFNGNPPLK-EKTGNWKACPFILGNECCERLAYYGIAGNLITYLTTKLHQGNVSAATNV 89

Query: 111 NNFLGISQASSVLGGFIADAYLGRYWTIAIFTTIYLGGLIGITLCATISAFVPNQEKCNE 170
             + G    + ++G  +ADAY GRYWTIA F+ IY  G+  +TL A++ A  P +     
Sbjct: 90  TTWQGTCYLTPLIGAVLADAYWGRYWTIACFSGIYFIGMSALTLSASVPALKPAE----- 149

Query: 171 LSLLLGKCEPAKAWQMTYLYTVLYLTGFGAAGIRPCVSSFGADQFDEKSKDYKSHLDRFF 230
              +   C  A   Q    +  LYL   G  GI+PCVSSFGADQFD+     +     FF
Sbjct: 150 --CIGDFCPSATPAQYAMFFGGLYLIALGTGGIKPCVSSFGADQFDDTDSRERVRKASFF 209

Query: 231 NFFYLSVTTGAIVAFTAVVYIQIKHGWGAAFGSLAIAMGFSNVVFFLGTPLYRHRLPGGS 290
           N+FY S+  GA+V+ + +V+IQ   GWG  FG   + MG +   FF GTPLYR + PGGS
Sbjct: 210 NWFYFSINIGALVSSSLLVWIQENRGWGLGFGIPTVFMGLAIASFFFGTPLYRFQKPGGS 269

Query: 291 PLTRVAQVLVAAFKKRNASFSSSEMVGLYEVAGKHSAIKGSGKILHTNDFRCLDKAAL-- 350
           P+TR++QV+VA+F+K +        + LYE   K+SAI GS KI HT+D + LDKAA+  
Sbjct: 270 PITRISQVVVASFRKSSVKVPEDATL-LYETQDKNSAIAGSRKIEHTDDCQYLDKAAVIS 329

Query: 351 -ELKDDGGNPSPWRLCTVTQVEEVKILLKLIPIPACTIILNLVLTEYLTLSVQQAYTLNT 410
            E    G   + WRLCTVTQVEE+KIL+++ PI A  II + V  +  T+ VQQ   +N 
Sbjct: 330 EEESKSGDYSNSWRLCTVTQVEELKILIRMFPIWASGIIFSAVYAQMSTMFVQQGRAMNC 389

Query: 411 HIGRLKLPVTCMPVFPGLSIFLILSLYYSVFVPLSRRITGHPHGASQLQRVGIGLAISII 470
            IG  +LP   +  F   S+ + + LY    VPL+R+ TG   G +++QR+GIGL +S++
Sbjct: 390 KIGSFQLPPAALGTFDTASVIIWVPLYDRFIVPLARKFTGVDKGFTEIQRMGIGLFVSVL 449

Query: 471 SVAWAGVFERYRRNYAIKSGYEASFLTPMPNLSAYWLLIQYCLIGIAEVFCIVGLLEFLY 530
            +A A + E  R + A   G   S   P+P +S  W + QY ++G AEVF  +G LEF Y
Sbjct: 450 CMAAAAIVEIIRLHMANDLGLVESG-APVP-ISVLWQIPQYFILGAAEVFYFIGQLEFFY 509

Query: 531 EEAPDAMKSIGSSYAALAGGLGCFAASLLNSIIKSVTGNPKEGTPSWLSQNINKGQFDYF 590
           +++PDAM+S+ S+ A L   LG + +SL+ +++   T   + G   W+S N+N G  DYF
Sbjct: 510 DQSPDAMRSLCSALALLTNALGNYLSSLILTLVTYFT--TRNGQEGWISDNLNSGHLDYF 569

Query: 591 YWLLTVMSVINFCIFLYSAHRYKYRK 614
           +WLL  +S++N  ++ +SA RYK +K
Sbjct: 570 FWLLAGLSLVNMAVYFFSAARYKQKK 582

BLAST of Carg15415 vs. ExPASy Swiss-Prot
Match: Q93Z20 (Protein NRT1/ PTR FAMILY 8.5 OS=Arabidopsis thaliana OX=3702 GN=NPF8.5 PE=2 SV=1)

HSP 1 Score: 380.9 bits (977), Expect = 2.7e-104
Identity = 221/576 (38.37%), Postives = 330/576 (57.29%), Query Frame = 0

Query: 41  GRGYTGGTTPVNIRGKPIADLSKTGGWIAAFFIFGNEMAERMAYFGLSVNMVAFMFYVMH 100
           G   T     ++I G P     KTG W A  FI GNE  ER+AY+G++ N++ +    +H
Sbjct: 31  GLSSTAEDGSIDIYGNP-PSKKKTGNWKACPFILGNECCERLAYYGIAKNLITYYTSELH 90

Query: 101 MPFTTSSDAVNNFLGISQASSVLGGFIADAYLGRYWTIAIFTTIYLGGLIGITLCATISA 160
               +++  V  + G    + ++G  IAD+Y GRYWTIA F+ IY  G+  +TL A++  
Sbjct: 91  ESNVSAASDVMIWQGTCYITPLIGAVIADSYWGRYWTIASFSAIYFIGMALLTLSASLPV 150

Query: 161 FVPNQEKCNELSLLLGKCEPAKAWQMTYLYTVLYLTGFGAAGIRPCVSSFGADQFDEKSK 220
             P    C  ++  L  C PA   Q    +T LYL   G  GI+PCVSSFGADQFD+   
Sbjct: 151 LKP--AACAGVAAAL--CSPATTVQYAVFFTGLYLIALGTGGIKPCVSSFGADQFDDTDP 210

Query: 221 DYKSHLDRFFNFFYLSVTTGAIVAFTAVVYIQIKHGWGAAFGSLAIAMGFSNVVFFLGTP 280
             +     FFN+FY S+  G+ ++ T +V++Q   GWG  F    + MG S   FF+GTP
Sbjct: 211 RERVRKASFFNWFYFSINIGSFISSTLLVWVQENVGWGLGFLIPTVFMGVSIASFFIGTP 270

Query: 281 LYRHRLPGGSPLTRVAQVLVAAFKKRNASFSSSEMVGLYEVAGKHSAIKGSGKILHTNDF 340
           LYR + PGGSP+TRV QVLVAA++K   +    ++  LYE   K+S I GS KI HT+ +
Sbjct: 271 LYRFQKPGGSPITRVCQVLVAAYRKLKLNL-PEDISFLYETREKNSMIAGSRKIQHTDGY 330

Query: 341 RCLDKAALELK---DDGGNPSPWRLCTVTQVEEVKILLKLIPIPACTIILNLVLTEYLTL 400
           + LDKAA+  +     G   +PW+LCTVTQVEEVK L+++ PI A  I+ +++ ++  TL
Sbjct: 331 KFLDKAAVISEYESKSGAFSNPWKLCTVTQVEEVKTLIRMFPIWASGIVYSVLYSQISTL 390

Query: 401 SVQQAYTLNTHIGRLKLPVTCMPVFPGLSIFLILSLYYSVFVPLSRRITGHPHGASQLQR 460
            VQQ  ++N  I   ++P     VF  L + + + +Y    VP  RR TG P G + LQR
Sbjct: 391 FVQQGRSMNRIIRSFEIPPASFGVFDTLIVLISIPIYDRFLVPFVRRFTGIPKGLTDLQR 450

Query: 461 VGIGLAISIISVAWAGVFERYRRNYAIKSGYEASFLTPMPNLSAYWLLIQYCLIGIAEVF 520
           +GIGL +S++S+A A + E  R   A        F+     +S +W + QY L+GIAEVF
Sbjct: 451 MGIGLFLSVLSIAAAAIVETVRLQLA------QDFVA----MSIFWQIPQYILMGIAEVF 510

Query: 521 CIVGLLEFLYEEAPDAMKSIGSSYAALAGGLGCFAASLLNSIIKSVTGNPKEGTPSWLSQ 580
             +G +EF Y+E+PDAM+S+ S+ A L   +G + +SL+ +++   T     G   W+  
Sbjct: 511 FFIGRVEFFYDESPDAMRSVCSALALLNTAVGSYLSSLILTLVAYFTA--LGGKDGWVPD 570

Query: 581 NINKGQFDYFYWLLTVMSVINFCIFLYSAHRYKYRK 614
           ++NKG  DYF+WLL  + ++N  ++     ++  +K
Sbjct: 571 DLNKGHLDYFFWLLVSLGLVNIPVYALICVKHTKKK 588

BLAST of Carg15415 vs. ExPASy TrEMBL
Match: A0A6J1F2P1 (protein NRT1/ PTR FAMILY 6.1 OS=Cucurbita moschata OX=3662 GN=LOC111439221 PE=3 SV=1)

HSP 1 Score: 1248.8 bits (3230), Expect = 0.0e+00
Identity = 626/630 (99.37%), Postives = 628/630 (99.68%), Query Frame = 0

Query: 1   MGSTEIKSPQEMVEMPARLDEQRKKLGMFFVESDDRRTAFGRGYTGGTTPVNIRGKPIAD 60
           MGSTEIKSPQEMVEMPARLDEQRKKLGMFFVESDDRRTAFGRGYTGGTTPVNIRGKPIAD
Sbjct: 1   MGSTEIKSPQEMVEMPARLDEQRKKLGMFFVESDDRRTAFGRGYTGGTTPVNIRGKPIAD 60

Query: 61  LSKTGGWIAAFFIFGNEMAERMAYFGLSVNMVAFMFYVMHMPFTTSSDAVNNFLGISQAS 120
           LSKTGGWIAAFFIFGNEMAERMAYFGLSVNMVAFMFYVMHMPFTTSSDAVNNFLGISQAS
Sbjct: 61  LSKTGGWIAAFFIFGNEMAERMAYFGLSVNMVAFMFYVMHMPFTTSSDAVNNFLGISQAS 120

Query: 121 SVLGGFIADAYLGRYWTIAIFTTIYLGGLIGITLCATISAFVPNQEKCNELSLLLGKCEP 180
           SVLGGFIADAYLGRYWTIAIFTTIYLGGLIGITLCATISAFVPNQE CNELSLLLGKCEP
Sbjct: 121 SVLGGFIADAYLGRYWTIAIFTTIYLGGLIGITLCATISAFVPNQEICNELSLLLGKCEP 180

Query: 181 AKAWQMTYLYTVLYLTGFGAAGIRPCVSSFGADQFDEKSKDYKSHLDRFFNFFYLSVTTG 240
           AKAWQMTYLYTVLYLTGFGAAGIRPCVSSFGADQFDEKSKDYKSHLDRFFNFFYLSVTTG
Sbjct: 181 AKAWQMTYLYTVLYLTGFGAAGIRPCVSSFGADQFDEKSKDYKSHLDRFFNFFYLSVTTG 240

Query: 241 AIVAFTAVVYIQIKHGWGAAFGSLAIAMGFSNVVFFLGTPLYRHRLPGGSPLTRVAQVLV 300
           AIVAFTAVVYIQIKHGWGAAFGSLAIAMGFSNVVFFLGTPLYRHRLPGGSPLTRVAQVLV
Sbjct: 241 AIVAFTAVVYIQIKHGWGAAFGSLAIAMGFSNVVFFLGTPLYRHRLPGGSPLTRVAQVLV 300

Query: 301 AAFKKRNASFSSSEMVGLYEVAGKHSAIKGSGKILHTNDFRCLDKAALELKDDGGNPSPW 360
           AAFKKRNASFSSSEMVGLYEVAGKHSAIKGSGKILHTNDFRCLDKAALELKDDGGNPSPW
Sbjct: 301 AAFKKRNASFSSSEMVGLYEVAGKHSAIKGSGKILHTNDFRCLDKAALELKDDGGNPSPW 360

Query: 361 RLCTVTQVEEVKILLKLIPIPACTIILNLVLTEYLTLSVQQAYTLNTHIGRLKLPVTCMP 420
           RLCTVTQVEEVKILLKLIPIPACTIILNLVLTEYLTLSVQQAYTLNTHIGRLKLPVTCMP
Sbjct: 361 RLCTVTQVEEVKILLKLIPIPACTIILNLVLTEYLTLSVQQAYTLNTHIGRLKLPVTCMP 420

Query: 421 VFPGLSIFLILSLYYSVFVPLSRRITGHPHGASQLQRVGIGLAISIISVAWAGVFERYRR 480
           VFPGLSIFLILSLYYSVFVPLSRRITGHPHGASQLQRVGIGLAISIISVAWAGVFERYRR
Sbjct: 421 VFPGLSIFLILSLYYSVFVPLSRRITGHPHGASQLQRVGIGLAISIISVAWAGVFERYRR 480

Query: 481 NYAIKSGYEASFLTPMPNLSAYWLLIQYCLIGIAEVFCIVGLLEFLYEEAPDAMKSIGSS 540
           NYAIK+GYEASFLTPMPNLSAYWLLIQYCLIGIAEVFCIVGLLEFLYEEAPDAMKSIGSS
Sbjct: 481 NYAIKNGYEASFLTPMPNLSAYWLLIQYCLIGIAEVFCIVGLLEFLYEEAPDAMKSIGSS 540

Query: 541 YAALAGGLGCFAASLLNSIIKSVTGNPKEGTPSWLSQNINKGQFDYFYWLLTVMSVINFC 600
           YAALAGGLGCFAASLLNSIIKSVTGN KEGTPSWLSQNINKGQFDYFYWLLTVMS+INFC
Sbjct: 541 YAALAGGLGCFAASLLNSIIKSVTGNTKEGTPSWLSQNINKGQFDYFYWLLTVMSIINFC 600

Query: 601 IFLYSAHRYKYRKDHEVGEGIMENGRHGTM 631
           IFLYSAHRYKYRKDHEVGEGIMENGRHGTM
Sbjct: 601 IFLYSAHRYKYRKDHEVGEGIMENGRHGTM 630

BLAST of Carg15415 vs. ExPASy TrEMBL
Match: A0A6J1I4E7 (protein NRT1/ PTR FAMILY 6.1 OS=Cucurbita maxima OX=3661 GN=LOC111470868 PE=3 SV=1)

HSP 1 Score: 1243.4 bits (3216), Expect = 0.0e+00
Identity = 622/630 (98.73%), Postives = 627/630 (99.52%), Query Frame = 0

Query: 1   MGSTEIKSPQEMVEMPARLDEQRKKLGMFFVESDDRRTAFGRGYTGGTTPVNIRGKPIAD 60
           MGSTEIKSPQEMVEMPARLDEQRKKLGMFFVESDDRRTAFGRGYTGGTTPVNIRGKPIAD
Sbjct: 1   MGSTEIKSPQEMVEMPARLDEQRKKLGMFFVESDDRRTAFGRGYTGGTTPVNIRGKPIAD 60

Query: 61  LSKTGGWIAAFFIFGNEMAERMAYFGLSVNMVAFMFYVMHMPFTTSSDAVNNFLGISQAS 120
           LSKTGGW AAFFIFGNEMAERMAYFGLSVNMVAFMFYVMHMPFTTSSDAVNNFLGISQAS
Sbjct: 61  LSKTGGWTAAFFIFGNEMAERMAYFGLSVNMVAFMFYVMHMPFTTSSDAVNNFLGISQAS 120

Query: 121 SVLGGFIADAYLGRYWTIAIFTTIYLGGLIGITLCATISAFVPNQEKCNELSLLLGKCEP 180
           SVLGGFIADAYLGRYWTIAIFTTIYLGGL GITLCATISAFVPNQ+ C+ELSLLLGKCEP
Sbjct: 121 SVLGGFIADAYLGRYWTIAIFTTIYLGGLTGITLCATISAFVPNQKICDELSLLLGKCEP 180

Query: 181 AKAWQMTYLYTVLYLTGFGAAGIRPCVSSFGADQFDEKSKDYKSHLDRFFNFFYLSVTTG 240
           AKAWQMTYLYTVLYLTGFGAAGIRPCVSSFGADQFDEKSKDYKSHLDRFFNFFYLSVTTG
Sbjct: 181 AKAWQMTYLYTVLYLTGFGAAGIRPCVSSFGADQFDEKSKDYKSHLDRFFNFFYLSVTTG 240

Query: 241 AIVAFTAVVYIQIKHGWGAAFGSLAIAMGFSNVVFFLGTPLYRHRLPGGSPLTRVAQVLV 300
           AIVAFTAVVYIQIKHGWGAAFGSLAIAMGFSNVVFFLGTPLYRHRLPGGSPLTRVAQVLV
Sbjct: 241 AIVAFTAVVYIQIKHGWGAAFGSLAIAMGFSNVVFFLGTPLYRHRLPGGSPLTRVAQVLV 300

Query: 301 AAFKKRNASFSSSEMVGLYEVAGKHSAIKGSGKILHTNDFRCLDKAALELKDDGGNPSPW 360
           AAF+KRNASFSSSEMVGLYEVAGKHSAIKGSGKILHTNDFRCLDKAALELKDDGGNPSPW
Sbjct: 301 AAFRKRNASFSSSEMVGLYEVAGKHSAIKGSGKILHTNDFRCLDKAALELKDDGGNPSPW 360

Query: 361 RLCTVTQVEEVKILLKLIPIPACTIILNLVLTEYLTLSVQQAYTLNTHIGRLKLPVTCMP 420
           RLCTVTQVEEVKILLKLIPIPACTIILNLVLTEYLTLSVQQAYTLNTH+GRLKLPVTCMP
Sbjct: 361 RLCTVTQVEEVKILLKLIPIPACTIILNLVLTEYLTLSVQQAYTLNTHMGRLKLPVTCMP 420

Query: 421 VFPGLSIFLILSLYYSVFVPLSRRITGHPHGASQLQRVGIGLAISIISVAWAGVFERYRR 480
           VFPGLSIFLILSLYYSVFVPLSRRITGHPHGASQLQRVGIGLAISIISVAWAGVFERYRR
Sbjct: 421 VFPGLSIFLILSLYYSVFVPLSRRITGHPHGASQLQRVGIGLAISIISVAWAGVFERYRR 480

Query: 481 NYAIKSGYEASFLTPMPNLSAYWLLIQYCLIGIAEVFCIVGLLEFLYEEAPDAMKSIGSS 540
           NYAIKSGYEASFLTPMPNLSAYWLLIQYCLIGIAEVFCIVGLLEFLYEEAPDAMKSIGSS
Sbjct: 481 NYAIKSGYEASFLTPMPNLSAYWLLIQYCLIGIAEVFCIVGLLEFLYEEAPDAMKSIGSS 540

Query: 541 YAALAGGLGCFAASLLNSIIKSVTGNPKEGTPSWLSQNINKGQFDYFYWLLTVMSVINFC 600
           YAALAGGLGCFAASLLNSIIKSVTGNPKEGTPSWLSQNINKGQFDYFYWLLTVMS+INFC
Sbjct: 541 YAALAGGLGCFAASLLNSIIKSVTGNPKEGTPSWLSQNINKGQFDYFYWLLTVMSIINFC 600

Query: 601 IFLYSAHRYKYRKDHEVGEGIMENGRHGTM 631
           IFLYSAHRYKYRKDHEVGEGIMENGRHGTM
Sbjct: 601 IFLYSAHRYKYRKDHEVGEGIMENGRHGTM 630

BLAST of Carg15415 vs. ExPASy TrEMBL
Match: A0A0A0KYN3 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_4G007610 PE=3 SV=1)

HSP 1 Score: 1163.7 bits (3009), Expect = 0.0e+00
Identity = 581/637 (91.21%), Postives = 608/637 (95.45%), Query Frame = 0

Query: 1   MGSTEIKSPQEMVEMP--ARLDE-----QRKKLGMFFVESDDRRTAFGRGYTGGTTPVNI 60
           MGS EIKS  E+ E P   RLDE     QRKKLGMFF+ESDDRRTAFGRGYTGGTTPVNI
Sbjct: 1   MGSREIKSLHEVNETPVLGRLDEHSESFQRKKLGMFFIESDDRRTAFGRGYTGGTTPVNI 60

Query: 61  RGKPIADLSKTGGWIAAFFIFGNEMAERMAYFGLSVNMVAFMFYVMHMPFTTSSDAVNNF 120
           RGKPIADLSKTGGWIAAFFIFGNEMAERMAYFGLSVNMVAFMFYVMHMPFTTSSDAVNNF
Sbjct: 61  RGKPIADLSKTGGWIAAFFIFGNEMAERMAYFGLSVNMVAFMFYVMHMPFTTSSDAVNNF 120

Query: 121 LGISQASSVLGGFIADAYLGRYWTIAIFTTIYLGGLIGITLCATISAFVPNQEKCNELSL 180
           LGISQASSVLGGF+ADAYLGRYWTIAIFTTIYLGGL+GITLCATIS FVPNQ KC++LSL
Sbjct: 121 LGISQASSVLGGFLADAYLGRYWTIAIFTTIYLGGLVGITLCATISTFVPNQAKCDQLSL 180

Query: 181 LLGKCEPAKAWQMTYLYTVLYLTGFGAAGIRPCVSSFGADQFDEKSKDYKSHLDRFFNFF 240
           LLG+CEPAK WQMTYLYTVLYLTGFGAAGIRPCVSSFGADQFDEKSKDYKSHLDRFFNFF
Sbjct: 181 LLGRCEPAKPWQMTYLYTVLYLTGFGAAGIRPCVSSFGADQFDEKSKDYKSHLDRFFNFF 240

Query: 241 YLSVTTGAIVAFTAVVYIQIKHGWGAAFGSLAIAMGFSNVVFFLGTPLYRHRLPGGSPLT 300
           YLSVT GAI+AFTAVVYIQIKHGWGAAFGSLAIAMGFSNVVFFLGTPLYRHRLPGGSPLT
Sbjct: 241 YLSVTVGAIIAFTAVVYIQIKHGWGAAFGSLAIAMGFSNVVFFLGTPLYRHRLPGGSPLT 300

Query: 301 RVAQVLVAAFKKRNASFSSSEMVGLYEVAGKHSAIKGSGKILHTNDFRCLDKAALELKDD 360
           RVAQVLVAA++KRNASFS+SE VGL+EV GK SAIKGSGKILHT+DFRCLDKAAL+LK+D
Sbjct: 301 RVAQVLVAAYRKRNASFSNSEFVGLFEVPGKQSAIKGSGKILHTDDFRCLDKAALQLKED 360

Query: 361 GGNPSPWRLCTVTQVEEVKILLKLIPIPACTIILNLVLTEYLTLSVQQAYTLNTHIGRLK 420
           GGNPSPW+LCTVTQVEEVKILLKLIPIPACTI+LNLVLTEYLTLSVQQAYT+NTHIGRLK
Sbjct: 361 GGNPSPWKLCTVTQVEEVKILLKLIPIPACTIMLNLVLTEYLTLSVQQAYTMNTHIGRLK 420

Query: 421 LPVTCMPVFPGLSIFLILSLYYSVFVPLSRRITGHPHGASQLQRVGIGLAISIISVAWAG 480
           LPVTCMPVFPGLSIFLILSLYYSVFVPLSRRITGHPHGASQLQRVGIGLAISI+SVAWAG
Sbjct: 421 LPVTCMPVFPGLSIFLILSLYYSVFVPLSRRITGHPHGASQLQRVGIGLAISILSVAWAG 480

Query: 481 VFERYRRNYAIKSGYEASFLTPMPNLSAYWLLIQYCLIGIAEVFCIVGLLEFLYEEAPDA 540
            FERYRRN+AI+SGYEASFL+PMPNLSAYWLLIQYCLIGIAEVFCIVGLLEFLYEEAPDA
Sbjct: 481 AFERYRRNFAIRSGYEASFLSPMPNLSAYWLLIQYCLIGIAEVFCIVGLLEFLYEEAPDA 540

Query: 541 MKSIGSSYAALAGGLGCFAASLLNSIIKSVTGNPKEGTPSWLSQNINKGQFDYFYWLLTV 600
           MKSIGS+YAALAGGLGCFAASLLNSIIKSVTG+P    PSWLSQNIN G+FDYFYWLLTV
Sbjct: 541 MKSIGSAYAALAGGLGCFAASLLNSIIKSVTGSPNGRNPSWLSQNINTGRFDYFYWLLTV 600

Query: 601 MSVINFCIFLYSAHRYKYRKDHEVGEGIMENGRHGTM 631
           MS+INFCIFLYSAH+YKYRKDHEVGEGIMENG H  M
Sbjct: 601 MSIINFCIFLYSAHKYKYRKDHEVGEGIMENGMHDKM 637

BLAST of Carg15415 vs. ExPASy TrEMBL
Match: A0A5A7TN06 (Protein NRT1/ PTR FAMILY 6.1 OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold46G002320 PE=3 SV=1)

HSP 1 Score: 1161.0 bits (3002), Expect = 0.0e+00
Identity = 579/637 (90.89%), Postives = 608/637 (95.45%), Query Frame = 0

Query: 1   MGSTEIKSPQEMVEMP--ARLDE-----QRKKLGMFFVESDDRRTAFGRGYTGGTTPVNI 60
           MGS EIKS QE+ E P   RLDE     QRKKLGMFF+ESDDRRTAFGRGYTGGTTPVNI
Sbjct: 1   MGSREIKSLQEVNETPVLGRLDEHSESFQRKKLGMFFIESDDRRTAFGRGYTGGTTPVNI 60

Query: 61  RGKPIADLSKTGGWIAAFFIFGNEMAERMAYFGLSVNMVAFMFYVMHMPFTTSSDAVNNF 120
           RGKPI DLSKTGGWIAAFFIFGNEMAERMAYFGLSVNMVAFMFYVMHMPFT+SSDAVNNF
Sbjct: 61  RGKPIVDLSKTGGWIAAFFIFGNEMAERMAYFGLSVNMVAFMFYVMHMPFTSSSDAVNNF 120

Query: 121 LGISQASSVLGGFIADAYLGRYWTIAIFTTIYLGGLIGITLCATISAFVPNQEKCNELSL 180
           LGISQASSVLGGF+ADAYLGRYWTIAIFTTIYLGGL+GITLCATIS FVPNQ KC++LSL
Sbjct: 121 LGISQASSVLGGFLADAYLGRYWTIAIFTTIYLGGLVGITLCATISTFVPNQAKCDQLSL 180

Query: 181 LLGKCEPAKAWQMTYLYTVLYLTGFGAAGIRPCVSSFGADQFDEKSKDYKSHLDRFFNFF 240
           LLG+CEPAK+WQMTYLYTVLYLTGFGAAGIRPCVSSFGADQFDEKSKDYKSHLDRFFNFF
Sbjct: 181 LLGRCEPAKSWQMTYLYTVLYLTGFGAAGIRPCVSSFGADQFDEKSKDYKSHLDRFFNFF 240

Query: 241 YLSVTTGAIVAFTAVVYIQIKHGWGAAFGSLAIAMGFSNVVFFLGTPLYRHRLPGGSPLT 300
           YLSVT GAI+AFTAVVYIQI+HGWGAAFGSLAIAMGFSNVVFFLGTPLYRHRLPGGSPLT
Sbjct: 241 YLSVTVGAIIAFTAVVYIQIQHGWGAAFGSLAIAMGFSNVVFFLGTPLYRHRLPGGSPLT 300

Query: 301 RVAQVLVAAFKKRNASFSSSEMVGLYEVAGKHSAIKGSGKILHTNDFRCLDKAALELKDD 360
           RVAQVLVAA++KRNASFS+SE VGLYEV GK SAIKGSGKILHT+DFRCLDKAAL+LK+D
Sbjct: 301 RVAQVLVAAYRKRNASFSNSEFVGLYEVPGKQSAIKGSGKILHTDDFRCLDKAALQLKED 360

Query: 361 GGNPSPWRLCTVTQVEEVKILLKLIPIPACTIILNLVLTEYLTLSVQQAYTLNTHIGRLK 420
           GGNPSPW+LCTVTQVEEVKILLKLIPIPACTI+LNLVLTEYLTLSVQQAYT+NTHIGRLK
Sbjct: 361 GGNPSPWKLCTVTQVEEVKILLKLIPIPACTIMLNLVLTEYLTLSVQQAYTMNTHIGRLK 420

Query: 421 LPVTCMPVFPGLSIFLILSLYYSVFVPLSRRITGHPHGASQLQRVGIGLAISIISVAWAG 480
           LPVTCMPVFPGLSIFLILSLYYSVFVPLSRRITGHPHGASQLQRVGIGLAISI+SVAWAG
Sbjct: 421 LPVTCMPVFPGLSIFLILSLYYSVFVPLSRRITGHPHGASQLQRVGIGLAISILSVAWAG 480

Query: 481 VFERYRRNYAIKSGYEASFLTPMPNLSAYWLLIQYCLIGIAEVFCIVGLLEFLYEEAPDA 540
            FERYRRN+A++SGYEASFL+PMPNLSAYWLLIQYCLIGIAEVFCIVGLLEFLYEEAPDA
Sbjct: 481 AFERYRRNFAVRSGYEASFLSPMPNLSAYWLLIQYCLIGIAEVFCIVGLLEFLYEEAPDA 540

Query: 541 MKSIGSSYAALAGGLGCFAASLLNSIIKSVTGNPKEGTPSWLSQNINKGQFDYFYWLLTV 600
           MKSIGS+YAALAGGLGCFAASLLNSIIKSVTG+     PSWLSQNIN G+FDYFYWLLTV
Sbjct: 541 MKSIGSAYAALAGGLGCFAASLLNSIIKSVTGSTNGRNPSWLSQNINTGRFDYFYWLLTV 600

Query: 601 MSVINFCIFLYSAHRYKYRKDHEVGEGIMENGRHGTM 631
           MS+INFCIFLYSAHRYKYRKDHEVGEGIMENG H  M
Sbjct: 601 MSIINFCIFLYSAHRYKYRKDHEVGEGIMENGMHDKM 637

BLAST of Carg15415 vs. ExPASy TrEMBL
Match: A0A1S3BXF6 (protein NRT1/ PTR FAMILY 6.1 OS=Cucumis melo OX=3656 GN=LOC103494642 PE=3 SV=1)

HSP 1 Score: 1160.6 bits (3001), Expect = 0.0e+00
Identity = 579/637 (90.89%), Postives = 608/637 (95.45%), Query Frame = 0

Query: 1   MGSTEIKSPQEMVEMP--ARLDE-----QRKKLGMFFVESDDRRTAFGRGYTGGTTPVNI 60
           MGS EIKS QE+ E P   RLDE     QRKKLGMFF+ESDDRRTAFGRGYTGGTTPVNI
Sbjct: 1   MGSREIKSLQEVNETPVLGRLDEHSESFQRKKLGMFFIESDDRRTAFGRGYTGGTTPVNI 60

Query: 61  RGKPIADLSKTGGWIAAFFIFGNEMAERMAYFGLSVNMVAFMFYVMHMPFTTSSDAVNNF 120
           RGKPI DLSKTGGWIAAFFIFGNEMAERMAYFGLSVNMVAFMFYVMHMPFT+SSDAVNNF
Sbjct: 61  RGKPIVDLSKTGGWIAAFFIFGNEMAERMAYFGLSVNMVAFMFYVMHMPFTSSSDAVNNF 120

Query: 121 LGISQASSVLGGFIADAYLGRYWTIAIFTTIYLGGLIGITLCATISAFVPNQEKCNELSL 180
           LGISQASSVLGGF+ADAYLGRYWTIAIFTTIYLGGL+GITLCATIS FVPNQ KC++LSL
Sbjct: 121 LGISQASSVLGGFLADAYLGRYWTIAIFTTIYLGGLVGITLCATISRFVPNQAKCDQLSL 180

Query: 181 LLGKCEPAKAWQMTYLYTVLYLTGFGAAGIRPCVSSFGADQFDEKSKDYKSHLDRFFNFF 240
           LLG+CEPAK+WQMTYLYTVLYLTGFGAAGIRPCVSSFGADQFDEKSKDYKSHLDRFFNFF
Sbjct: 181 LLGRCEPAKSWQMTYLYTVLYLTGFGAAGIRPCVSSFGADQFDEKSKDYKSHLDRFFNFF 240

Query: 241 YLSVTTGAIVAFTAVVYIQIKHGWGAAFGSLAIAMGFSNVVFFLGTPLYRHRLPGGSPLT 300
           YLSVT GAI+AFTAVVYIQI+HGWGAAFGSLAIAMGFSNVVFFLGTPLYRHRLPGGSPLT
Sbjct: 241 YLSVTVGAIIAFTAVVYIQIQHGWGAAFGSLAIAMGFSNVVFFLGTPLYRHRLPGGSPLT 300

Query: 301 RVAQVLVAAFKKRNASFSSSEMVGLYEVAGKHSAIKGSGKILHTNDFRCLDKAALELKDD 360
           RVAQVLVAA++KRNASFS+SE VGLYEV GK SAIKGSGKILHT+DFRCLDKAAL+LK+D
Sbjct: 301 RVAQVLVAAYRKRNASFSNSEFVGLYEVPGKQSAIKGSGKILHTDDFRCLDKAALQLKED 360

Query: 361 GGNPSPWRLCTVTQVEEVKILLKLIPIPACTIILNLVLTEYLTLSVQQAYTLNTHIGRLK 420
           GGNPSPW+LCTVTQVEEVKILLKLIPIPACTI+LNLVLTEYLTLSVQQAYT+NTHIGRLK
Sbjct: 361 GGNPSPWKLCTVTQVEEVKILLKLIPIPACTIMLNLVLTEYLTLSVQQAYTMNTHIGRLK 420

Query: 421 LPVTCMPVFPGLSIFLILSLYYSVFVPLSRRITGHPHGASQLQRVGIGLAISIISVAWAG 480
           LPVTCMPVFPGLSIFLILSLYYSVFVPLSRRITGHPHGASQLQRVGIGLAISI+SVAWAG
Sbjct: 421 LPVTCMPVFPGLSIFLILSLYYSVFVPLSRRITGHPHGASQLQRVGIGLAISILSVAWAG 480

Query: 481 VFERYRRNYAIKSGYEASFLTPMPNLSAYWLLIQYCLIGIAEVFCIVGLLEFLYEEAPDA 540
            FERYRRN+A++SGYEASFL+PMPNLSAYWLLIQYCLIGIAEVFCIVGLLEFLYEEAPDA
Sbjct: 481 AFERYRRNFAVRSGYEASFLSPMPNLSAYWLLIQYCLIGIAEVFCIVGLLEFLYEEAPDA 540

Query: 541 MKSIGSSYAALAGGLGCFAASLLNSIIKSVTGNPKEGTPSWLSQNINKGQFDYFYWLLTV 600
           MKSIGS+YAALAGGLGCFAASLLNSIIKSVTG+     PSWLSQNIN G+FDYFYWLLTV
Sbjct: 541 MKSIGSAYAALAGGLGCFAASLLNSIIKSVTGSTNGRNPSWLSQNINTGRFDYFYWLLTV 600

Query: 601 MSVINFCIFLYSAHRYKYRKDHEVGEGIMENGRHGTM 631
           MS+INFCIFLYSAHRYKYRKDHEVGEGIMENG H  M
Sbjct: 601 MSIINFCIFLYSAHRYKYRKDHEVGEGIMENGMHDKM 637

BLAST of Carg15415 vs. TAIR 10
Match: AT5G13400.1 (Major facilitator superfamily protein )

HSP 1 Score: 977.6 bits (2526), Expect = 4.6e-285
Identity = 487/613 (79.45%), Postives = 549/613 (89.56%), Query Frame = 0

Query: 1   MGSTEIKSPQEMVEMPARLD-EQRKKLGMFFVESDDRRTAFGRGYTGGTTPVNIRGKPIA 60
           M ++EIKSP  + E P       RK+L +FF+ESD+RR A GRGYTGGTTPVNI GKPIA
Sbjct: 1   MVASEIKSPVSVPETPGSSSVHHRKQLSVFFIESDNRRLALGRGYTGGTTPVNIHGKPIA 60

Query: 61  DLSKTGGWIAAFFIFGNEMAERMAYFGLSVNMVAFMFYVMHMPFTTSSDAVNNFLGISQA 120
           +LSKTGGWIAAFFIFGNEMAERMAYFGLSVNMVAFMFYVMH PF +SS+AVNNFLGISQA
Sbjct: 61  NLSKTGGWIAAFFIFGNEMAERMAYFGLSVNMVAFMFYVMHRPFESSSNAVNNFLGISQA 120

Query: 121 SSVLGGFIADAYLGRYWTIAIFTTIYLGGLIGITLCATISAFVPNQEKCNELSLLLGKCE 180
           SSVLGGF+ADAYLGRYWTIAIFTT+YL GLIGITL A++  FVP+Q  C +LSLLLG CE
Sbjct: 121 SSVLGGFLADAYLGRYWTIAIFTTMYLVGLIGITLGASLKMFVPDQSNCGQLSLLLGNCE 180

Query: 181 PAKAWQMTYLYTVLYLTGFGAAGIRPCVSSFGADQFDEKSKDYKSHLDRFFNFFYLSVTT 240
            AK+WQM YLYTVLY+TGFGAAGIRPCVSSFGADQFDEKSKDYK+HLDRFFNFFYLSVT 
Sbjct: 181 EAKSWQMLYLYTVLYITGFGAAGIRPCVSSFGADQFDEKSKDYKTHLDRFFNFFYLSVTL 240

Query: 241 GAIVAFTAVVYIQIKHGWGAAFGSLAIAMGFSNVVFFLGTPLYRHRLPGGSPLTRVAQVL 300
           GAI+AFT VVY+Q++ GWG AFG+LA+AMG SN +FF GTPLYRHRLPGGSPLTRVAQVL
Sbjct: 241 GAIIAFTLVVYVQMELGWGMAFGTLAVAMGISNALFFAGTPLYRHRLPGGSPLTRVAQVL 300

Query: 301 VAAFKKRNASFSSSEMVGLYEVAGKHSAIKGSGKILHTNDFRCLDKAALELKDDGGNPSP 360
           VAAF+KRNA+F+SSE +GLYEV G  SAI GS KI H+NDF  LDKAALELK+DG  PSP
Sbjct: 301 VAAFRKRNAAFTSSEFIGLYEVPGLKSAINGSRKIPHSNDFIWLDKAALELKEDGLEPSP 360

Query: 361 WRLCTVTQVEEVKILLKLIPIPACTIILNLVLTEYLTLSVQQAYTLNTHIGRLKLPVTCM 420
           W+LCTVTQVEEVKIL++LIPIP CTI+L+LVLTEYLTLSVQQAYTLNTHI  LKLPVTCM
Sbjct: 361 WKLCTVTQVEEVKILIRLIPIPTCTIMLSLVLTEYLTLSVQQAYTLNTHIQHLKLPVTCM 420

Query: 421 PVFPGLSIFLILSLYYSVFVPLSRRITGHPHGASQLQRVGIGLAISIISVAWAGVFERYR 480
           PVFPGLSIFLILSLYYSVFVP++RRITG+PHGASQLQRVGIGLA+SIISVAWAG+FE YR
Sbjct: 421 PVFPGLSIFLILSLYYSVFVPITRRITGNPHGASQLQRVGIGLAVSIISVAWAGLFENYR 480

Query: 481 RNYAIKSGYEASFLTPMPNLSAYWLLIQYCLIGIAEVFCIVGLLEFLYEEAPDAMKSIGS 540
           R+YAI++G+E +FLT MP+L+AYWLLIQYCLIGIAEVFCIVGLLEFLYEEAPDAMKSIGS
Sbjct: 481 RHYAIQNGFEFNFLTQMPDLTAYWLLIQYCLIGIAEVFCIVGLLEFLYEEAPDAMKSIGS 540

Query: 541 SYAALAGGLGCFAASLLNSIIKSVTGNPKEGTPSWLSQNINKGQFDYFYWLLTVMSVINF 600
           +YAALAGGLGCFAA++LN+I+K+ T +      SWLSQNIN G+FD  YWLLT++S +NF
Sbjct: 541 AYAALAGGLGCFAATILNNIVKAATRD--SDGKSWLSQNINTGRFDCLYWLLTLLSFLNF 600

Query: 601 CIFLYSAHRYKYR 613
           C+FL+SAHRYKYR
Sbjct: 601 CVFLWSAHRYKYR 611

BLAST of Carg15415 vs. TAIR 10
Match: AT3G54140.1 (peptide transporter 1 )

HSP 1 Score: 416.8 bits (1070), Expect = 3.2e-116
Identity = 227/568 (39.96%), Postives = 346/568 (60.92%), Query Frame = 0

Query: 51  VNIRGKPIADLSKTGGWIAAFFIFGNEMAERMAYFGLSVNMVAFMFYVMHMPFTTSSDAV 110
           V+I   P A+  KTG W A  FI GNE  ER+AY+G+  N+V ++   ++    T+++ V
Sbjct: 13  VDIHKNP-ANKEKTGNWKACRFILGNECCERLAYYGMGTNLVNYLESRLNQGNATAANNV 72

Query: 111 NNFLGISQASSVLGGFIADAYLGRYWTIAIFTTIYLGGLIGITLCATISAFVPNQEKCNE 170
            N+ G    + ++G FIADAYLGRYWTIA F  IY+ G+  +TL A++    P    CN 
Sbjct: 73  TNWSGTCYITPLIGAFIADAYLGRYWTIATFVFIYVSGMTLLTLSASVPGLKPG--NCN- 132

Query: 171 LSLLLGKCEPAKAWQMTYLYTVLYLTGFGAAGIRPCVSSFGADQFDEKSKDYKSHLDRFF 230
                  C P  + Q    +  LY+   G  GI+PCVSSFGADQFDE  ++ K     FF
Sbjct: 133 ----ADTCHPNSS-QTAVFFVALYMIALGTGGIKPCVSSFGADQFDENDENEKIKKSSFF 192

Query: 231 NFFYLSVTTGAIVAFTAVVYIQIKHGWGAAFGSLAIAMGFSNVVFFLGTPLYRHRLPGGS 290
           N+FY S+  GA++A T +V+IQ+  GWG  FG   +AM  +   FF G+  YR + PGGS
Sbjct: 193 NWFYFSINVGALIAATVLVWIQMNVGWGWGFGVPTVAMVIAVCFFFFGSRFYRLQRPGGS 252

Query: 291 PLTRVAQVLVAAFKKRNASFSSSEMVGLYEVAGKHSAIKGSGKILHTNDFRCLDKAALEL 350
           PLTR+ QV+VAAF+K +      + + L+E A   S IKGS K++HT++ +  DKAA+E 
Sbjct: 253 PLTRIFQVIVAAFRKISVKVPEDKSL-LFETADDESNIKGSRKLVHTDNLKFFDKAAVES 312

Query: 351 KDDG---GNPSPWRLCTVTQVEEVKILLKLIPIPACTIILNLVLTEYLTLSVQQAYTLNT 410
           + D    G  +PWRLC+VTQVEE+K ++ L+P+ A  I+   V ++  T+ V Q  T++ 
Sbjct: 313 QSDSIKDGEVNPWRLCSVTQVEELKSIITLLPVWATGIVFATVYSQMSTMFVLQGNTMDQ 372

Query: 411 HIGR-LKLPVTCMPVFPGLSIFLILSLYYSVFVPLSRRITGHPHGASQLQRVGIGLAISI 470
           H+G+  ++P   + +F  +S+     +Y    +PL+R+ T +  G +QLQR+GIGL +SI
Sbjct: 373 HMGKNFEIPSASLSLFDTVSVLFWTPVYDQFIIPLARKFTRNERGFTQLQRMGIGLVVSI 432

Query: 471 ISVAWAGVFERYRRNYA-IKSGYEASFLTPMPNLSAYWLLIQYCLIGIAEVFCIVGLLEF 530
            ++  AGV E  R +Y    + Y+   +    ++S +W + QY LIG AEVF  +G LEF
Sbjct: 433 FAMITAGVLEVVRLDYVKTHNAYDQKQI----HMSIFWQIPQYLLIGCAEVFTFIGQLEF 492

Query: 531 LYEEAPDAMKSIGSSYAALAGGLGCFAASLLNSIIKSVTGNPKEGTPSWLSQNINKGQFD 590
            Y++APDAM+S+ S+ +     LG + +++L +++  +T   K G P W+  N+N+G  D
Sbjct: 493 FYDQAPDAMRSLCSALSLTTVALGNYLSTVLVTVVMKIT--KKNGKPGWIPDNLNRGHLD 552

Query: 591 YFYWLLTVMSVINFCIFLYSAHRYKYRK 614
           YF++LL  +S +NF ++L+ + RYKY+K
Sbjct: 553 YFFYLLATLSFLNFLVYLWISKRYKYKK 564

BLAST of Carg15415 vs. TAIR 10
Match: AT5G01180.1 (peptide transporter 5 )

HSP 1 Score: 408.3 bits (1048), Expect = 1.1e-113
Identity = 231/567 (40.74%), Postives = 336/567 (59.26%), Query Frame = 0

Query: 51  VNIRGKPIADLSKTGGWIAAFFIFGNEMAERMAYFGLSVNMVAFMFYVMHMPFTTSSDAV 110
           ++I  KP A+ +KTG W A  FI G E  ER+AY+G+S N++ ++   M+M   ++S +V
Sbjct: 14  LDIHKKP-ANKNKTGTWKACRFILGTECCERLAYYGMSTNLINYLEKQMNMENVSASKSV 73

Query: 111 NNFLGISQASSVLGGFIADAYLGRYWTIAIFTTIYLGGLIGITLCATISAFVPNQEKCNE 170
           +N+ G   A+ ++G FIADAYLGRYWTIA F  IY+ G+  +T+ A++    P    C+ 
Sbjct: 74  SNWSGTCYATPLIGAFIADAYLGRYWTIASFVVIYIAGMTLLTISASVPGLTPT---CS- 133

Query: 171 LSLLLGKCEPAKAWQMTYLYTVLYLTGFGAAGIRPCVSSFGADQFDEKSKDYKSHLDRFF 230
                G+   A A Q    +  LYL   G  GI+PCVSSFGADQFD+  +  K     FF
Sbjct: 134 -----GETCHATAGQTAITFIALYLIALGTGGIKPCVSSFGADQFDDTDEKEKESKSSFF 193

Query: 231 NFFYLSVTTGAIVAFTAVVYIQIKHGWGAAFGSLAIAMGFSNVVFFLGTPLYRHRLPGGS 290
           N+FY  +  GA++A + +V+IQ+  GWG   G   +AM  + V FF G+  YR + PGGS
Sbjct: 194 NWFYFVINVGAMIASSVLVWIQMNVGWGWGLGVPTVAMAIAVVFFFAGSNFYRLQKPGGS 253

Query: 291 PLTRVAQVLVAAFKKRNASFSSSEMVGLYEVAGKHSAIKGSGKILHTNDFRCLDKAALEL 350
           PLTR+ QV+VA+ +K        E + LYE     S+I GS K+ HT      DKAA+E 
Sbjct: 254 PLTRMLQVIVASCRKSKVKIPEDESL-LYENQDAESSIIGSRKLEHTKILTFFDKAAVET 313

Query: 351 KDD---GGNPSPWRLCTVTQVEEVKILLKLIPIPACTIILNLVLTEYLTLSVQQAYTLNT 410
           + D       S W+LCTVTQVEE+K L++L+PI A  I+   V ++  T+ V Q  TL+ 
Sbjct: 314 ESDNKGAAKSSSWKLCTVTQVEELKALIRLLPIWATGIVFASVYSQMGTVFVLQGNTLDQ 373

Query: 411 HIG-RLKLPVTCMPVFPGLSIFLILSLYYSVFVPLSRRITGHPHGASQLQRVGIGLAISI 470
           H+G   K+P   + +F  LS+     +Y  + VP +R+ TGH  G +QLQR+GIGL ISI
Sbjct: 374 HMGPNFKIPSASLSLFDTLSVLFWAPVYDKLIVPFARKYTGHERGFTQLQRIGIGLVISI 433

Query: 471 ISVAWAGVFERYRRNYAIKSGYEASFLTPMPNLSAYWLLIQYCLIGIAEVFCIVGLLEFL 530
            S+  AG+ E  R NY            PM   + +W + QY L+G AEVF  +G LEF 
Sbjct: 434 FSMVSAGILEVARLNYVQTHNLYNEETIPM---TIFWQVPQYFLVGCAEVFTFIGQLEFF 493

Query: 531 YEEAPDAMKSIGSSYAALAGGLGCFAASLLNSIIKSVTGNPKEGTPSWLSQNINKGQFDY 590
           Y++APDAM+S+ S+ +  A   G + ++ L +++  VT +   G P W+++N+N G  DY
Sbjct: 494 YDQAPDAMRSLCSALSLTAIAFGNYLSTFLVTLVTKVTRS--GGRPGWIAKNLNNGHLDY 553

Query: 591 FYWLLTVMSVINFCIFLYSAHRYKYRK 614
           F+WLL  +S +NF ++L+ A  Y Y+K
Sbjct: 554 FFWLLAGLSFLNFLVYLWIAKWYTYKK 564

BLAST of Carg15415 vs. TAIR 10
Match: AT2G02040.1 (peptide transporter 2 )

HSP 1 Score: 407.9 bits (1047), Expect = 1.5e-113
Identity = 228/566 (40.28%), Postives = 334/566 (59.01%), Query Frame = 0

Query: 51  VNIRGKPIADLSKTGGWIAAFFIFGNEMAERMAYFGLSVNMVAFMFYVMHMPFTTSSDAV 110
           V+  G P     KTG W A  FI GNE  ER+AY+G++ N++ ++   +H    +++  V
Sbjct: 30  VDFNGNPPLK-EKTGNWKACPFILGNECCERLAYYGIAGNLITYLTTKLHQGNVSAATNV 89

Query: 111 NNFLGISQASSVLGGFIADAYLGRYWTIAIFTTIYLGGLIGITLCATISAFVPNQEKCNE 170
             + G    + ++G  +ADAY GRYWTIA F+ IY  G+  +TL A++ A  P +     
Sbjct: 90  TTWQGTCYLTPLIGAVLADAYWGRYWTIACFSGIYFIGMSALTLSASVPALKPAE----- 149

Query: 171 LSLLLGKCEPAKAWQMTYLYTVLYLTGFGAAGIRPCVSSFGADQFDEKSKDYKSHLDRFF 230
              +   C  A   Q    +  LYL   G  GI+PCVSSFGADQFD+     +     FF
Sbjct: 150 --CIGDFCPSATPAQYAMFFGGLYLIALGTGGIKPCVSSFGADQFDDTDSRERVRKASFF 209

Query: 231 NFFYLSVTTGAIVAFTAVVYIQIKHGWGAAFGSLAIAMGFSNVVFFLGTPLYRHRLPGGS 290
           N+FY S+  GA+V+ + +V+IQ   GWG  FG   + MG +   FF GTPLYR + PGGS
Sbjct: 210 NWFYFSINIGALVSSSLLVWIQENRGWGLGFGIPTVFMGLAIASFFFGTPLYRFQKPGGS 269

Query: 291 PLTRVAQVLVAAFKKRNASFSSSEMVGLYEVAGKHSAIKGSGKILHTNDFRCLDKAAL-- 350
           P+TR++QV+VA+F+K +        + LYE   K+SAI GS KI HT+D + LDKAA+  
Sbjct: 270 PITRISQVVVASFRKSSVKVPEDATL-LYETQDKNSAIAGSRKIEHTDDCQYLDKAAVIS 329

Query: 351 -ELKDDGGNPSPWRLCTVTQVEEVKILLKLIPIPACTIILNLVLTEYLTLSVQQAYTLNT 410
            E    G   + WRLCTVTQVEE+KIL+++ PI A  II + V  +  T+ VQQ   +N 
Sbjct: 330 EEESKSGDYSNSWRLCTVTQVEELKILIRMFPIWASGIIFSAVYAQMSTMFVQQGRAMNC 389

Query: 411 HIGRLKLPVTCMPVFPGLSIFLILSLYYSVFVPLSRRITGHPHGASQLQRVGIGLAISII 470
            IG  +LP   +  F   S+ + + LY    VPL+R+ TG   G +++QR+GIGL +S++
Sbjct: 390 KIGSFQLPPAALGTFDTASVIIWVPLYDRFIVPLARKFTGVDKGFTEIQRMGIGLFVSVL 449

Query: 471 SVAWAGVFERYRRNYAIKSGYEASFLTPMPNLSAYWLLIQYCLIGIAEVFCIVGLLEFLY 530
            +A A + E  R + A   G   S   P+P +S  W + QY ++G AEVF  +G LEF Y
Sbjct: 450 CMAAAAIVEIIRLHMANDLGLVESG-APVP-ISVLWQIPQYFILGAAEVFYFIGQLEFFY 509

Query: 531 EEAPDAMKSIGSSYAALAGGLGCFAASLLNSIIKSVTGNPKEGTPSWLSQNINKGQFDYF 590
           +++PDAM+S+ S+ A L   LG + +SL+ +++   T   + G   W+S N+N G  DYF
Sbjct: 510 DQSPDAMRSLCSALALLTNALGNYLSSLILTLVTYFT--TRNGQEGWISDNLNSGHLDYF 569

Query: 591 YWLLTVMSVINFCIFLYSAHRYKYRK 614
           +WLL  +S++N  ++ +SA RYK +K
Sbjct: 570 FWLLAGLSLVNMAVYFFSAARYKQKK 582

BLAST of Carg15415 vs. TAIR 10
Match: AT1G62200.1 (Major facilitator superfamily protein )

HSP 1 Score: 380.9 bits (977), Expect = 1.9e-105
Identity = 221/576 (38.37%), Postives = 330/576 (57.29%), Query Frame = 0

Query: 41  GRGYTGGTTPVNIRGKPIADLSKTGGWIAAFFIFGNEMAERMAYFGLSVNMVAFMFYVMH 100
           G   T     ++I G P     KTG W A  FI GNE  ER+AY+G++ N++ +    +H
Sbjct: 31  GLSSTAEDGSIDIYGNP-PSKKKTGNWKACPFILGNECCERLAYYGIAKNLITYYTSELH 90

Query: 101 MPFTTSSDAVNNFLGISQASSVLGGFIADAYLGRYWTIAIFTTIYLGGLIGITLCATISA 160
               +++  V  + G    + ++G  IAD+Y GRYWTIA F+ IY  G+  +TL A++  
Sbjct: 91  ESNVSAASDVMIWQGTCYITPLIGAVIADSYWGRYWTIASFSAIYFIGMALLTLSASLPV 150

Query: 161 FVPNQEKCNELSLLLGKCEPAKAWQMTYLYTVLYLTGFGAAGIRPCVSSFGADQFDEKSK 220
             P    C  ++  L  C PA   Q    +T LYL   G  GI+PCVSSFGADQFD+   
Sbjct: 151 LKP--AACAGVAAAL--CSPATTVQYAVFFTGLYLIALGTGGIKPCVSSFGADQFDDTDP 210

Query: 221 DYKSHLDRFFNFFYLSVTTGAIVAFTAVVYIQIKHGWGAAFGSLAIAMGFSNVVFFLGTP 280
             +     FFN+FY S+  G+ ++ T +V++Q   GWG  F    + MG S   FF+GTP
Sbjct: 211 RERVRKASFFNWFYFSINIGSFISSTLLVWVQENVGWGLGFLIPTVFMGVSIASFFIGTP 270

Query: 281 LYRHRLPGGSPLTRVAQVLVAAFKKRNASFSSSEMVGLYEVAGKHSAIKGSGKILHTNDF 340
           LYR + PGGSP+TRV QVLVAA++K   +    ++  LYE   K+S I GS KI HT+ +
Sbjct: 271 LYRFQKPGGSPITRVCQVLVAAYRKLKLNL-PEDISFLYETREKNSMIAGSRKIQHTDGY 330

Query: 341 RCLDKAALELK---DDGGNPSPWRLCTVTQVEEVKILLKLIPIPACTIILNLVLTEYLTL 400
           + LDKAA+  +     G   +PW+LCTVTQVEEVK L+++ PI A  I+ +++ ++  TL
Sbjct: 331 KFLDKAAVISEYESKSGAFSNPWKLCTVTQVEEVKTLIRMFPIWASGIVYSVLYSQISTL 390

Query: 401 SVQQAYTLNTHIGRLKLPVTCMPVFPGLSIFLILSLYYSVFVPLSRRITGHPHGASQLQR 460
            VQQ  ++N  I   ++P     VF  L + + + +Y    VP  RR TG P G + LQR
Sbjct: 391 FVQQGRSMNRIIRSFEIPPASFGVFDTLIVLISIPIYDRFLVPFVRRFTGIPKGLTDLQR 450

Query: 461 VGIGLAISIISVAWAGVFERYRRNYAIKSGYEASFLTPMPNLSAYWLLIQYCLIGIAEVF 520
           +GIGL +S++S+A A + E  R   A        F+     +S +W + QY L+GIAEVF
Sbjct: 451 MGIGLFLSVLSIAAAAIVETVRLQLA------QDFVA----MSIFWQIPQYILMGIAEVF 510

Query: 521 CIVGLLEFLYEEAPDAMKSIGSSYAALAGGLGCFAASLLNSIIKSVTGNPKEGTPSWLSQ 580
             +G +EF Y+E+PDAM+S+ S+ A L   +G + +SL+ +++   T     G   W+  
Sbjct: 511 FFIGRVEFFYDESPDAMRSVCSALALLNTAVGSYLSSLILTLVAYFTA--LGGKDGWVPD 570

Query: 581 NINKGQFDYFYWLLTVMSVINFCIFLYSAHRYKYRK 614
           ++NKG  DYF+WLL  + ++N  ++     ++  +K
Sbjct: 571 DLNKGHLDYFFWLLVSLGLVNIPVYALICVKHTKKK 588

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
KAG7029147.10.0e+00100.00Protein NRT1/ PTR FAMILY 6.1 [Cucurbita argyrosperma subsp. argyrosperma][more]
XP_022932773.10.0e+0099.37protein NRT1/ PTR FAMILY 6.1 [Cucurbita moschata][more]
XP_022972287.10.0e+0098.73protein NRT1/ PTR FAMILY 6.1 [Cucurbita maxima][more]
XP_023538659.10.0e+0098.73protein NRT1/ PTR FAMILY 6.1 [Cucurbita pepo subsp. pepo][more]
KAG6597701.10.0e+0095.40Protein NRT1/ PTR FAMILY 6.1, partial [Cucurbita argyrosperma subsp. sororia][more]
Match NameE-valueIdentityDescription
Q9LYR66.5e-28479.45Protein NRT1/ PTR FAMILY 6.1 OS=Arabidopsis thaliana OX=3702 GN=NPF6.1 PE=2 SV=1[more]
Q9M3904.4e-11539.96Protein NRT1/ PTR FAMILY 8.1 OS=Arabidopsis thaliana OX=3702 GN=NPF8.1 PE=1 SV=1[more]
Q9LFB81.6e-11240.74Protein NRT1/ PTR FAMILY 8.2 OS=Arabidopsis thaliana OX=3702 GN=NPF8.2 PE=2 SV=1[more]
P460322.1e-11240.28Protein NRT1/ PTR FAMILY 8.3 OS=Arabidopsis thaliana OX=3702 GN=NPF8.3 PE=1 SV=1[more]
Q93Z202.7e-10438.37Protein NRT1/ PTR FAMILY 8.5 OS=Arabidopsis thaliana OX=3702 GN=NPF8.5 PE=2 SV=1[more]
Match NameE-valueIdentityDescription
A0A6J1F2P10.0e+0099.37protein NRT1/ PTR FAMILY 6.1 OS=Cucurbita moschata OX=3662 GN=LOC111439221 PE=3 ... [more]
A0A6J1I4E70.0e+0098.73protein NRT1/ PTR FAMILY 6.1 OS=Cucurbita maxima OX=3661 GN=LOC111470868 PE=3 SV... [more]
A0A0A0KYN30.0e+0091.21Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_4G007610 PE=3 SV=1[more]
A0A5A7TN060.0e+0090.89Protein NRT1/ PTR FAMILY 6.1 OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_sca... [more]
A0A1S3BXF60.0e+0090.89protein NRT1/ PTR FAMILY 6.1 OS=Cucumis melo OX=3656 GN=LOC103494642 PE=3 SV=1[more]
Match NameE-valueIdentityDescription
AT5G13400.14.6e-28579.45Major facilitator superfamily protein [more]
AT3G54140.13.2e-11639.96peptide transporter 1 [more]
AT5G01180.11.1e-11340.74peptide transporter 5 [more]
AT2G02040.11.5e-11340.28peptide transporter 2 [more]
AT1G62200.11.9e-10538.37Major facilitator superfamily protein [more]
InterPro
Analysis Name: InterPro Annotations of Silver-seed gourd (SMH-JMG-627) v2
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR036259MFS transporter superfamilyGENE3D1.20.1250.20MFS general substrate transporter like domainscoord: 46..617
e-value: 2.3E-162
score: 542.9
IPR036259MFS transporter superfamilySUPERFAMILY103473MFS general substrate transportercoord: 62..613
IPR000109Proton-dependent oligopeptide transporter familyPFAMPF00854PTR2coord: 135..566
e-value: 1.3E-80
score: 271.2
IPR000109Proton-dependent oligopeptide transporter familyPANTHERPTHR11654OLIGOPEPTIDE TRANSPORTER-RELATEDcoord: 56..612
NoneNo IPR availablePANTHERPTHR11654:SF112PROTEIN NRT1/ PTR FAMILY 6.1coord: 56..612
NoneNo IPR availableCDDcd17351MFS_NPFcoord: 70..608
e-value: 0.0
score: 546.836
IPR018456PTR2 family proton/oligopeptide symporter, conserved sitePROSITEPS01022PTR2_1coord: 124..148

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Carg15415-RACarg15415-RAmRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0006857 oligopeptide transport
biological_process GO:0055085 transmembrane transport
cellular_component GO:0016021 integral component of membrane
cellular_component GO:0016020 membrane
molecular_function GO:0022857 transmembrane transporter activity