Carg15395 (gene) Silver-seed gourd (SMH-JMG-627) v2

Overview
NameCarg15395
Typegene
OrganismCucurbita argyrosperma subsp. argyrosperma cv. SMH-JMG-627 (Silver-seed gourd (SMH-JMG-627) v2)
Descriptiontwo-pore potassium channel 3-like
LocationCarg_Chr03: 9568600 .. 9572269 (+)
RNA-Seq ExpressionCarg15395
SyntenyCarg15395
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRCDSpolypeptidethree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGAAGCTATTTCTGAAATGTTGGATAAGTACAAAAAAGAAGGAAGAAAAGAAATTCGAAGGGAAAAGAAATTCGATTCCAGTTGGGTTGGGTGATCAGCTGTGGATGATCAATGCTCAAGCTCGAGCGAATTGGGTTTCTTTTTCTTCATCGGATCGTGCCTCATTAATCGCTTCTTCATTAATCCTTCGTCTTCCTCCGCCGTCGCAGTGCGTATGCTGCTACGGCGCTCTGTAATGTAATTATGCCACTGGGTTTCCCCCGTTCTTCACCCTTTTGGAGGCATTCAGTTTCTGGGTCCCAACAGTTTACTCCCATTTGAGCTTTCGCTCTATCTATCCCACGTTGTCTTTGTGACTTGAAATTCATTCACCAAAATCGTTTCCTGGGTTTTCATTTTGACGCGTTCTTCTTCTTGTCCTACGATTGGACCCAACGCTGCTTGTTGCACTGTGGCCTCAGAGGTTAACCAGCATCATATCTGAATGGAGAAAGAGCCTCTTCTTCCGTATATGAGCCCAAGGGGGAAGCCGCTTCCTCCACCTCCTCAATTATTATGTCCACTCCCTGAAAACGATGAAATTACTCTCCCTATGACACCCACGGAGTTCAAAGACCGCCTGATCTTCGGTCCTTCATCGTCTCCGCAAGACACTTCTCCCATATTCGACGCCTTGACGCTCTCCCTTTCCTCCTCAAGACCCTCTGCTTCATCTTCTCTTCAGGACCCATCTACACCTTTGGCCCCAAAATCCCAATCGCCGTCTCAACAGGCATGGCTTGTGGATTCCGATTACTCATGGCACAAAAGCAACCTCCACCGCTCCAAAACCGCACCTGCCATGGCCGTCATCAACGATGTGAACCATTCGCAAGAGCCCAAACCACAATTTGGTAAGCAATCTATTATCCGCCAAGCTGTTGTTCTTCTAATTCTTTACTTATCATTGGGCGTGCTTATCTATTGGATTAATCGTGATAAGTTTTCGGCTCACGAGACTCACCCTGTGGTCGATGCATTGTACTTTTGCATAGTAACTATGTGCACTATTGGGTATGGAGATATCACGCCCAATAGTGCTGCGACGAAGCTGTTCTCCGTATTGTTTGTGTTGGTGGGATTCGGATTTATTGATATATTACTCTCTGGAATGGTCAGTTATGTGCTTGATTTGCAGGAGAGTTATTTGTTAAGGAATGTAAAGAGAGGGGTGCAGCGTGAATCTGGGAAATCCTATATAATTGATGTGAAGAAGGGAAGAATGAGGATTAGAATGAAAGTGGCTCTGGCCTTGGGTGTTGTGGTTCTTTGTATAGGACTTGGCATGGGTGTTATGCATTTTGTTGAAAATCTGGGTTGGTTGGATTCATTTTATCTTTCAGTTATGTCTGTCACAACAGTTGGATATGGTGATCAGGCGTTCAAGTCGATGACTGGTCGTATTTTTGCTTCGATTTGGTTGCTTGTATCGACCCTTGCTGTCGCTCGGGCGTTTCTTTATTTGGCAGAGGCAAGGGTAGATAAGCGGCATAGGATGATGGCAAAGTGGATACTTGGTCAGGATATGACTGTCTCTGAGTTTCTTGCTGCTGATACCGACAACAATGGCTGTGTGAGGTATGTATTAACATTATTCTTTCAAACTAAGTTTAGAGAAGTAGCTTAGAAAATTGGTCTGGATGTTACTGATTCTGCTTGCTGAGATGTCTTACTTGTATTGTTTCATGAACACGCATGAGCATTTTTTGCATAACTTGTATGAAATTGCCTACCCACTTTGCTGTGTTTGAAGAGCCACAGGGGCTAACAGCCCAAACCCACTACCCGACTACCGCTAGCAGAAAGTGTCCTCTTTGGACTTTCCTTTCGAGGGCTCCTCCTCAAGGTTTTAAAACGCGTTTGTTAGGGAGAGGTTTCTACACCCCTTTAAGGAATGTTTCGTTCCCCTCTCCAACCAACGTGGGATCTCACACAACTTATTCCTCCTTGGGGGCCTAACATCCTCGCTGGTACAACGTCCAGTGTTTGCCTCTGATACCATTTGTAATAGCCCAAGCTCACTGCTAGCAGATACTGTCTTCTTTGGTCTTTCCCTTCAAGAGCATCCCCTCAAGGTTTTAATACGCGTCTGTTAGAGAGGTGTTTTCACACCCCTATAAGGAATGTTTCGTTCCCCTCTCCAACCAATGCGAGATTCTACAATTTAATACGTGATTTAACTGTTTGGTACGTAGGTTCAAAAGTCCCACGGTTGAACGAAATCTGTGAGTGTAGTTTGTTAGCCTGTAAAGGTTCTGGCTTCTGAATACCAAATAGTTCTTCATTTGATACAAGTTGAGAAAAGACATCTGAGAAATCTCTCTCCCATGGGTCTTCTGGCAGGTCTCTCACTTCTTTTCTTTAACACGAAATGCCTCCTCTTTAAACTAATGAAAAAGAATAAAAGTTCAAGGAAATTGATAGGTCATGTGCTATGAGAATTGGGTGCATGTGCTATGAGAATGTGTTCTGTTATGAGGGTGGGTCATTAGTTAGCTATCAATGCAAGCAGGGCCGAATAAGAATATCGTTAAAAACTTGAATACGCTAGCTTGAGTCGGGTTCTTATTTTTGGCATTAAGTATTGGATTTGGTATCTTGAGAGATGGGAATTCTGGAATTCTTTGCTGACAATGAACCTATTTTCCTATCAACACTAAAAGAGTTCTTCTTCATCCTGATTCTGTACACGTTTCCATCAACGACGTCGTATGATGTGCACGCCTGCTAAACTTGTTGGAATCTTATTTCATGTCTTAAGTAAACAAGGCATAAGATCTTTGCCTCAACTTTTTTATATCTTCTGGTTTTACTCTGATTGAAACACGCTGCAACTTAGCTCATCCAAAACTGTCCATGATTCTTGTTTGAACATGTCTTTGTTCAATATGTATTTCAATCTCTATTTAGATTTCTAGTACCAGTTTGAGTTCCTTTGTTGAGAACATCTATGTTAGGCATTCCTTCCCATCCTGTACGGTTCGGTCCATGTTCTTCTGTCAACTTAAATATCGCAGCCATGTAGGTTTGGCTGGTACCTCAATCAGCACATGGCTTATTTTGTACACATATCGATGTTGTGAATCAAGTCGGGTAATATAAAACAGAACAACAAAACTGGGTGAGCTGACATTGGGTATTTTATGTCATCCATGTTGCAGTAGGTCAGAGTATGTGTTATACAAGCTGAAGGAGATGGGAAAGGTATCAGAGAAAGATGTGATGCAGATATCTAATAACTTTGATAGACTAGACACTGGCAACTGTGGGAAGATTACACTGGCCGATCTCTTGGAAAGTCATCACTGATATACAAACACAATACCGACAACGATTCCCTCGGCATTTGGATTCGGAAGGATTCGAGTCATGGATCGTCAAAGATTGAAAGAGAGAAAGAGCTTGAATTTCCTACAACATTTGTTTTGCTCCATTCATGTGATGATTCTGTACAAAGTTTTGATAGCATAGGAACTAGGAAGAATCTTCTGTAGAAACTAAAAGATAATCGTTTGAACTTGTTGAACTCTAATTTGTTTATTGGTTTATATCTTTGTATGTGTCCATAGCTCTGCATGACATAATGACTCATACTCTGAATTCGGATTAGAATTCAGCATGATCACATTA

mRNA sequence

ATGAAGCTATTTCTGAAATGTTGGATAAGTACAAAAAAGAAGGAAGAAAAGAAATTCGAAGGGAAAAGAAATTCGATTCCAGTTGGGTTGGGTGATCAGCTGTGGATGATCAATGCTCAAGCTCGAGCGAATTGGGTTTCTTTTTCTTCATCGGATCGTGCCTCATTAATCGCTTCTTCATTAATCCTTCGTCTTCCTCCGCCGTCGCAGTGCGTATGCTGCTACGGCGCTCTGTAATGTAATTATGCCACTGGGTTTCCCCCGTTCTTCACCCTTTTGGAGGCATTCAGTTTCTGGGTCCCAACAGTTTACTCCCATTTGAGCTTTCGCTCTATCTATCCCACGTTGTCTTTGTGACTTGAAATTCATTCACCAAAATCGTTTCCTGGGTTTTCATTTTGACGCGTTCTTCTTCTTGTCCTACGATTGGACCCAACGCTGCTTGTTGCACTGTGGCCTCAGAGGTTAACCAGCATCATATCTGAATGGAGAAAGAGCCTCTTCTTCCGTATATGAGCCCAAGGGGGAAGCCGCTTCCTCCACCTCCTCAATTATTATGTCCACTCCCTGAAAACGATGAAATTACTCTCCCTATGACACCCACGGAGTTCAAAGACCGCCTGATCTTCGGTCCTTCATCGTCTCCGCAAGACACTTCTCCCATATTCGACGCCTTGACGCTCTCCCTTTCCTCCTCAAGACCCTCTGCTTCATCTTCTCTTCAGGACCCATCTACACCTTTGGCCCCAAAATCCCAATCGCCGTCTCAACAGGCATGGCTTGTGGATTCCGATTACTCATGGCACAAAAGCAACCTCCACCGCTCCAAAACCGCACCTGCCATGGCCGTCATCAACGATGTGAACCATTCGCAAGAGCCCAAACCACAATTTGGTAAGCAATCTATTATCCGCCAAGCTGTTGTTCTTCTAATTCTTTACTTATCATTGGGCGTGCTTATCTATTGGATTAATCGTGATAAGTTTTCGGCTCACGAGACTCACCCTGTGGTCGATGCATTGTACTTTTGCATAGTAACTATGTGCACTATTGGGTATGGAGATATCACGCCCAATAGTGCTGCGACGAAGCTGTTCTCCGTATTGTTTGTGTTGGTGGGATTCGGATTTATTGATATATTACTCTCTGGAATGGTCAGTTATGTGCTTGATTTGCAGGAGAGTTATTTGTTAAGGAATGTAAAGAGAGGGGTGCAGCGTGAATCTGGGAAATCCTATATAATTGATGTGAAGAAGGGAAGAATGAGGATTAGAATGAAAGTGGCTCTGGCCTTGGGTGTTGTGGTTCTTTGTATAGGACTTGGCATGGGTGTTATGCATTTTGTTGAAAATCTGGGTTGGTTGGATTCATTTTATCTTTCAGTTATGTCTGTCACAACAGTTGGATATGGTGATCAGGCGTTCAAGTCGATGACTGGTCGTATTTTTGCTTCGATTTGGTTGCTTGTATCGACCCTTGCTGTCGCTCGGGCGTTTCTTTATTTGGCAGAGGCAAGGGTAGATAAGCGGCATAGGATGATGGCAAAGTGGATACTTGGTCAGGATATGACTGTCTCTGAGTTTCTTGCTGCTGATACCGACAACAATGGCTGTGTGAGTAGGTCAGAGTATGTGTTATACAAGCTGAAGGAGATGGGAAAGGTATCAGAGAAAGATGTGATGCAGATATCTAATAACTTTGATAGACTAGACACTGGCAACTGTGGGAAGATTACACTGGCCGATCTCTTGGAAAGTCATCACTGATATACAAACACAATACCGACAACGATTCCCTCGGCATTTGGATTCGGAAGGATTCGAGTCATGGATCGTCAAAGATTGAAAGAGAGAAAGAGCTTGAATTTCCTACAACATTTGTTTTGCTCCATTCATGTGATGATTCTGTACAAAGTTTTGATAGCATAGGAACTAGGAAGAATCTTCTGTAGAAACTAAAAGATAATCGTTTGAACTTGTTGAACTCTAATTTGTTTATTGGTTTATATCTTTGTATGTGTCCATAGCTCTGCATGACATAATGACTCATACTCTGAATTCGGATTAGAATTCAGCATGATCACATTA

Coding sequence (CDS)

ATGGAGAAAGAGCCTCTTCTTCCGTATATGAGCCCAAGGGGGAAGCCGCTTCCTCCACCTCCTCAATTATTATGTCCACTCCCTGAAAACGATGAAATTACTCTCCCTATGACACCCACGGAGTTCAAAGACCGCCTGATCTTCGGTCCTTCATCGTCTCCGCAAGACACTTCTCCCATATTCGACGCCTTGACGCTCTCCCTTTCCTCCTCAAGACCCTCTGCTTCATCTTCTCTTCAGGACCCATCTACACCTTTGGCCCCAAAATCCCAATCGCCGTCTCAACAGGCATGGCTTGTGGATTCCGATTACTCATGGCACAAAAGCAACCTCCACCGCTCCAAAACCGCACCTGCCATGGCCGTCATCAACGATGTGAACCATTCGCAAGAGCCCAAACCACAATTTGGTAAGCAATCTATTATCCGCCAAGCTGTTGTTCTTCTAATTCTTTACTTATCATTGGGCGTGCTTATCTATTGGATTAATCGTGATAAGTTTTCGGCTCACGAGACTCACCCTGTGGTCGATGCATTGTACTTTTGCATAGTAACTATGTGCACTATTGGGTATGGAGATATCACGCCCAATAGTGCTGCGACGAAGCTGTTCTCCGTATTGTTTGTGTTGGTGGGATTCGGATTTATTGATATATTACTCTCTGGAATGGTCAGTTATGTGCTTGATTTGCAGGAGAGTTATTTGTTAAGGAATGTAAAGAGAGGGGTGCAGCGTGAATCTGGGAAATCCTATATAATTGATGTGAAGAAGGGAAGAATGAGGATTAGAATGAAAGTGGCTCTGGCCTTGGGTGTTGTGGTTCTTTGTATAGGACTTGGCATGGGTGTTATGCATTTTGTTGAAAATCTGGGTTGGTTGGATTCATTTTATCTTTCAGTTATGTCTGTCACAACAGTTGGATATGGTGATCAGGCGTTCAAGTCGATGACTGGTCGTATTTTTGCTTCGATTTGGTTGCTTGTATCGACCCTTGCTGTCGCTCGGGCGTTTCTTTATTTGGCAGAGGCAAGGGTAGATAAGCGGCATAGGATGATGGCAAAGTGGATACTTGGTCAGGATATGACTGTCTCTGAGTTTCTTGCTGCTGATACCGACAACAATGGCTGTGTGAGTAGGTCAGAGTATGTGTTATACAAGCTGAAGGAGATGGGAAAGGTATCAGAGAAAGATGTGATGCAGATATCTAATAACTTTGATAGACTAGACACTGGCAACTGTGGGAAGATTACACTGGCCGATCTCTTGGAAAGTCATCACTGA

Protein sequence

MEKEPLLPYMSPRGKPLPPPPQLLCPLPENDEITLPMTPTEFKDRLIFGPSSSPQDTSPIFDALTLSLSSSRPSASSSLQDPSTPLAPKSQSPSQQAWLVDSDYSWHKSNLHRSKTAPAMAVINDVNHSQEPKPQFGKQSIIRQAVVLLILYLSLGVLIYWINRDKFSAHETHPVVDALYFCIVTMCTIGYGDITPNSAATKLFSVLFVLVGFGFIDILLSGMVSYVLDLQESYLLRNVKRGVQRESGKSYIIDVKKGRMRIRMKVALALGVVVLCIGLGMGVMHFVENLGWLDSFYLSVMSVTTVGYGDQAFKSMTGRIFASIWLLVSTLAVARAFLYLAEARVDKRHRMMAKWILGQDMTVSEFLAADTDNNGCVSRSEYVLYKLKEMGKVSEKDVMQISNNFDRLDTGNCGKITLADLLESHH
Homology
BLAST of Carg15395 vs. NCBI nr
Match: KAG6581409.1 (Two-pore potassium channel 3, partial [Cucurbita argyrosperma subsp. sororia] >KAG7034695.1 Two-pore potassium channel 3 [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 831.6 bits (2147), Expect = 3.0e-237
Identity = 426/426 (100.00%), Postives = 426/426 (100.00%), Query Frame = 0

Query: 1   MEKEPLLPYMSPRGKPLPPPPQLLCPLPENDEITLPMTPTEFKDRLIFGPSSSPQDTSPI 60
           MEKEPLLPYMSPRGKPLPPPPQLLCPLPENDEITLPMTPTEFKDRLIFGPSSSPQDTSPI
Sbjct: 1   MEKEPLLPYMSPRGKPLPPPPQLLCPLPENDEITLPMTPTEFKDRLIFGPSSSPQDTSPI 60

Query: 61  FDALTLSLSSSRPSASSSLQDPSTPLAPKSQSPSQQAWLVDSDYSWHKSNLHRSKTAPAM 120
           FDALTLSLSSSRPSASSSLQDPSTPLAPKSQSPSQQAWLVDSDYSWHKSNLHRSKTAPAM
Sbjct: 61  FDALTLSLSSSRPSASSSLQDPSTPLAPKSQSPSQQAWLVDSDYSWHKSNLHRSKTAPAM 120

Query: 121 AVINDVNHSQEPKPQFGKQSIIRQAVVLLILYLSLGVLIYWINRDKFSAHETHPVVDALY 180
           AVINDVNHSQEPKPQFGKQSIIRQAVVLLILYLSLGVLIYWINRDKFSAHETHPVVDALY
Sbjct: 121 AVINDVNHSQEPKPQFGKQSIIRQAVVLLILYLSLGVLIYWINRDKFSAHETHPVVDALY 180

Query: 181 FCIVTMCTIGYGDITPNSAATKLFSVLFVLVGFGFIDILLSGMVSYVLDLQESYLLRNVK 240
           FCIVTMCTIGYGDITPNSAATKLFSVLFVLVGFGFIDILLSGMVSYVLDLQESYLLRNVK
Sbjct: 181 FCIVTMCTIGYGDITPNSAATKLFSVLFVLVGFGFIDILLSGMVSYVLDLQESYLLRNVK 240

Query: 241 RGVQRESGKSYIIDVKKGRMRIRMKVALALGVVVLCIGLGMGVMHFVENLGWLDSFYLSV 300
           RGVQRESGKSYIIDVKKGRMRIRMKVALALGVVVLCIGLGMGVMHFVENLGWLDSFYLSV
Sbjct: 241 RGVQRESGKSYIIDVKKGRMRIRMKVALALGVVVLCIGLGMGVMHFVENLGWLDSFYLSV 300

Query: 301 MSVTTVGYGDQAFKSMTGRIFASIWLLVSTLAVARAFLYLAEARVDKRHRMMAKWILGQD 360
           MSVTTVGYGDQAFKSMTGRIFASIWLLVSTLAVARAFLYLAEARVDKRHRMMAKWILGQD
Sbjct: 301 MSVTTVGYGDQAFKSMTGRIFASIWLLVSTLAVARAFLYLAEARVDKRHRMMAKWILGQD 360

Query: 361 MTVSEFLAADTDNNGCVSRSEYVLYKLKEMGKVSEKDVMQISNNFDRLDTGNCGKITLAD 420
           MTVSEFLAADTDNNGCVSRSEYVLYKLKEMGKVSEKDVMQISNNFDRLDTGNCGKITLAD
Sbjct: 361 MTVSEFLAADTDNNGCVSRSEYVLYKLKEMGKVSEKDVMQISNNFDRLDTGNCGKITLAD 420

Query: 421 LLESHH 427
           LLESHH
Sbjct: 421 LLESHH 426

BLAST of Carg15395 vs. NCBI nr
Match: XP_023544618.1 (two-pore potassium channel 3-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 829.3 bits (2141), Expect = 1.5e-236
Identity = 424/426 (99.53%), Postives = 426/426 (100.00%), Query Frame = 0

Query: 1   MEKEPLLPYMSPRGKPLPPPPQLLCPLPENDEITLPMTPTEFKDRLIFGPSSSPQDTSPI 60
           MEKEPLLPYMSPRGKPLPPPPQLLCPLPENDEITLPMTPTEFKDRLIFGPSSSPQDTSPI
Sbjct: 1   MEKEPLLPYMSPRGKPLPPPPQLLCPLPENDEITLPMTPTEFKDRLIFGPSSSPQDTSPI 60

Query: 61  FDALTLSLSSSRPSASSSLQDPSTPLAPKSQSPSQQAWLVDSDYSWHKSNLHRSKTAPAM 120
           FDALTLSLSSSRPSASSSLQDPSTPLAPKSQSPSQQAWLVDS+YSWHKSNLHRSKTAPAM
Sbjct: 61  FDALTLSLSSSRPSASSSLQDPSTPLAPKSQSPSQQAWLVDSNYSWHKSNLHRSKTAPAM 120

Query: 121 AVINDVNHSQEPKPQFGKQSIIRQAVVLLILYLSLGVLIYWINRDKFSAHETHPVVDALY 180
           AVINDVNHSQEPKPQFGKQSIIRQAVVLLILYLSLGVLIYWINRDKFSAHETHPVVDALY
Sbjct: 121 AVINDVNHSQEPKPQFGKQSIIRQAVVLLILYLSLGVLIYWINRDKFSAHETHPVVDALY 180

Query: 181 FCIVTMCTIGYGDITPNSAATKLFSVLFVLVGFGFIDILLSGMVSYVLDLQESYLLRNVK 240
           FCIVTMCTIGYGDITPNSAATKLFSVLFVLVGFGFIDILLSGMVSYVLDLQESYLLRNVK
Sbjct: 181 FCIVTMCTIGYGDITPNSAATKLFSVLFVLVGFGFIDILLSGMVSYVLDLQESYLLRNVK 240

Query: 241 RGVQRESGKSYIIDVKKGRMRIRMKVALALGVVVLCIGLGMGVMHFVENLGWLDSFYLSV 300
           RGVQRESGKSYIIDVKKGRMRIRMKVALALGVV+LCIGLGMGVMHFVENLGWLDSFYLSV
Sbjct: 241 RGVQRESGKSYIIDVKKGRMRIRMKVALALGVVILCIGLGMGVMHFVENLGWLDSFYLSV 300

Query: 301 MSVTTVGYGDQAFKSMTGRIFASIWLLVSTLAVARAFLYLAEARVDKRHRMMAKWILGQD 360
           MSVTTVGYGDQAFKSMTGRIFASIWLLVSTLAVARAFLYLAEARVDKRHRMMAKWILGQD
Sbjct: 301 MSVTTVGYGDQAFKSMTGRIFASIWLLVSTLAVARAFLYLAEARVDKRHRMMAKWILGQD 360

Query: 361 MTVSEFLAADTDNNGCVSRSEYVLYKLKEMGKVSEKDVMQISNNFDRLDTGNCGKITLAD 420
           MTVSEFLAADTDNNGCVSRSEYVLYKLKEMGKVSEKDVMQISNNFDRLDTGNCGKITLAD
Sbjct: 361 MTVSEFLAADTDNNGCVSRSEYVLYKLKEMGKVSEKDVMQISNNFDRLDTGNCGKITLAD 420

Query: 421 LLESHH 427
           LLESHH
Sbjct: 421 LLESHH 426

BLAST of Carg15395 vs. NCBI nr
Match: XP_022926002.1 (two-pore potassium channel 3-like [Cucurbita moschata])

HSP 1 Score: 827.8 bits (2137), Expect = 4.3e-236
Identity = 423/426 (99.30%), Postives = 426/426 (100.00%), Query Frame = 0

Query: 1   MEKEPLLPYMSPRGKPLPPPPQLLCPLPENDEITLPMTPTEFKDRLIFGPSSSPQDTSPI 60
           MEKEPLLPY+SPRGKPLPPPPQLLCPLPENDEITLPMTPTEFKDRLIFGPSSSPQDTSPI
Sbjct: 1   MEKEPLLPYISPRGKPLPPPPQLLCPLPENDEITLPMTPTEFKDRLIFGPSSSPQDTSPI 60

Query: 61  FDALTLSLSSSRPSASSSLQDPSTPLAPKSQSPSQQAWLVDSDYSWHKSNLHRSKTAPAM 120
           FDALTLSLSSSRPSASSSLQDPSTPLAPKSQSPSQQAWLVDS+YSWHKSNLHRSKTAPAM
Sbjct: 61  FDALTLSLSSSRPSASSSLQDPSTPLAPKSQSPSQQAWLVDSNYSWHKSNLHRSKTAPAM 120

Query: 121 AVINDVNHSQEPKPQFGKQSIIRQAVVLLILYLSLGVLIYWINRDKFSAHETHPVVDALY 180
           AVINDVNHSQEPKPQFGKQSIIRQAVVLLILYLSLGVLIYWINRDKFSAHETHPVVDALY
Sbjct: 121 AVINDVNHSQEPKPQFGKQSIIRQAVVLLILYLSLGVLIYWINRDKFSAHETHPVVDALY 180

Query: 181 FCIVTMCTIGYGDITPNSAATKLFSVLFVLVGFGFIDILLSGMVSYVLDLQESYLLRNVK 240
           FCIVTMCTIGYGDITPNSAATKLFSVLFVLVGFGFIDILLSGMVSYVLDLQESYLLRNVK
Sbjct: 181 FCIVTMCTIGYGDITPNSAATKLFSVLFVLVGFGFIDILLSGMVSYVLDLQESYLLRNVK 240

Query: 241 RGVQRESGKSYIIDVKKGRMRIRMKVALALGVVVLCIGLGMGVMHFVENLGWLDSFYLSV 300
           RGVQRESGKSYIIDVKKGRMRIRMKVALALGVV+LCIGLGMGVMHFVENLGWLDSFYLSV
Sbjct: 241 RGVQRESGKSYIIDVKKGRMRIRMKVALALGVVILCIGLGMGVMHFVENLGWLDSFYLSV 300

Query: 301 MSVTTVGYGDQAFKSMTGRIFASIWLLVSTLAVARAFLYLAEARVDKRHRMMAKWILGQD 360
           MSVTTVGYGDQAFKSMTGRIFASIWLLVSTLAVARAFLYLAEARVDKRHRMMAKWILGQD
Sbjct: 301 MSVTTVGYGDQAFKSMTGRIFASIWLLVSTLAVARAFLYLAEARVDKRHRMMAKWILGQD 360

Query: 361 MTVSEFLAADTDNNGCVSRSEYVLYKLKEMGKVSEKDVMQISNNFDRLDTGNCGKITLAD 420
           MTVSEFLAADTDNNGCVSRSEYVLYKLKEMGKVSEKDVMQISNNFDRLDTGNCGKITLAD
Sbjct: 361 MTVSEFLAADTDNNGCVSRSEYVLYKLKEMGKVSEKDVMQISNNFDRLDTGNCGKITLAD 420

Query: 421 LLESHH 427
           LLESHH
Sbjct: 421 LLESHH 426

BLAST of Carg15395 vs. NCBI nr
Match: XP_022978331.1 (two-pore potassium channel 3-like [Cucurbita maxima])

HSP 1 Score: 826.6 bits (2134), Expect = 9.6e-236
Identity = 423/426 (99.30%), Postives = 425/426 (99.77%), Query Frame = 0

Query: 1   MEKEPLLPYMSPRGKPLPPPPQLLCPLPENDEITLPMTPTEFKDRLIFGPSSSPQDTSPI 60
           MEKEPLLPYMSPRGKPLPPPPQLLCPLPENDEITLPMTPTEFKDRLIFGPSSSPQDTSPI
Sbjct: 1   MEKEPLLPYMSPRGKPLPPPPQLLCPLPENDEITLPMTPTEFKDRLIFGPSSSPQDTSPI 60

Query: 61  FDALTLSLSSSRPSASSSLQDPSTPLAPKSQSPSQQAWLVDSDYSWHKSNLHRSKTAPAM 120
           FDALTLSLSSSRPSASSSLQDPSTPLAPKSQSPSQQAWLVDS+YSWHKSNLHRSKTAPAM
Sbjct: 61  FDALTLSLSSSRPSASSSLQDPSTPLAPKSQSPSQQAWLVDSNYSWHKSNLHRSKTAPAM 120

Query: 121 AVINDVNHSQEPKPQFGKQSIIRQAVVLLILYLSLGVLIYWINRDKFSAHETHPVVDALY 180
           AVINDVNHSQEPKPQFGK SIIRQAVVLLILYLSLGVLIYWINRDKFSAHETHPVVDALY
Sbjct: 121 AVINDVNHSQEPKPQFGKLSIIRQAVVLLILYLSLGVLIYWINRDKFSAHETHPVVDALY 180

Query: 181 FCIVTMCTIGYGDITPNSAATKLFSVLFVLVGFGFIDILLSGMVSYVLDLQESYLLRNVK 240
           FCIVTMCTIGYGDITPNSAATKLFSVLFVLVGFGFIDILLSGMVSYVLDLQESYLLRNVK
Sbjct: 181 FCIVTMCTIGYGDITPNSAATKLFSVLFVLVGFGFIDILLSGMVSYVLDLQESYLLRNVK 240

Query: 241 RGVQRESGKSYIIDVKKGRMRIRMKVALALGVVVLCIGLGMGVMHFVENLGWLDSFYLSV 300
           RGVQRESGKSYIIDVKKGRMRIRMKVALALGVV+LCIGLGMGVMHFVENLGWLDSFYLSV
Sbjct: 241 RGVQRESGKSYIIDVKKGRMRIRMKVALALGVVILCIGLGMGVMHFVENLGWLDSFYLSV 300

Query: 301 MSVTTVGYGDQAFKSMTGRIFASIWLLVSTLAVARAFLYLAEARVDKRHRMMAKWILGQD 360
           MSVTTVGYGDQAFKSMTGRIFASIWLLVSTLAVARAFLYLAEARVDKRHRMMAKWILGQD
Sbjct: 301 MSVTTVGYGDQAFKSMTGRIFASIWLLVSTLAVARAFLYLAEARVDKRHRMMAKWILGQD 360

Query: 361 MTVSEFLAADTDNNGCVSRSEYVLYKLKEMGKVSEKDVMQISNNFDRLDTGNCGKITLAD 420
           MTVSEFLAADTDNNGCVSRSEYVLYKLKEMGKVSEKDVMQISNNFDRLDTGNCGKITLAD
Sbjct: 361 MTVSEFLAADTDNNGCVSRSEYVLYKLKEMGKVSEKDVMQISNNFDRLDTGNCGKITLAD 420

Query: 421 LLESHH 427
           LLESHH
Sbjct: 421 LLESHH 426

BLAST of Carg15395 vs. NCBI nr
Match: XP_008439665.1 (PREDICTED: two-pore potassium channel 3 [Cucumis melo])

HSP 1 Score: 780.8 bits (2015), Expect = 6.0e-222
Identity = 400/426 (93.90%), Postives = 414/426 (97.18%), Query Frame = 0

Query: 1   MEKEPLLPYMSPRGKPLPPPPQLLCPLPENDEITLPMTPTEFKDRLIFGPSSSPQDTSPI 60
           MEKEPLLPY+SPRGKP PPPPQ LCPLPENDEITLPMTPTEFKDRLIFGPSSSPQD SPI
Sbjct: 1   MEKEPLLPYLSPRGKPPPPPPQ-LCPLPENDEITLPMTPTEFKDRLIFGPSSSPQDASPI 60

Query: 61  FDALTLSLSSSRPSASSSLQDPSTPLAPKSQSPSQQAWLVDSDYSWHKSNLHRSKTAPAM 120
           FDALTLSLSSSRPSASSSLQDPSTPL P+SQS SQQAWLVDS+Y+W KSNLHRSKTAPAM
Sbjct: 61  FDALTLSLSSSRPSASSSLQDPSTPLDPRSQSQSQQAWLVDSNYTWPKSNLHRSKTAPAM 120

Query: 121 AVINDVNHSQEPKPQFGKQSIIRQAVVLLILYLSLGVLIYWINRDKFSAHETHPVVDALY 180
           AVINDVNHSQEPKPQFGKQSIIRQAVVLLI+YLSLGVLIYW+NRD F+A ETHPVVDALY
Sbjct: 121 AVINDVNHSQEPKPQFGKQSIIRQAVVLLIVYLSLGVLIYWLNRDNFAAQETHPVVDALY 180

Query: 181 FCIVTMCTIGYGDITPNSAATKLFSVLFVLVGFGFIDILLSGMVSYVLDLQESYLLRNVK 240
           FCIVTMCTIGYGDITPNS ATKLFSVLFVLVGFGFIDILLSGMVSYVLDLQESYLLRNVK
Sbjct: 181 FCIVTMCTIGYGDITPNSTATKLFSVLFVLVGFGFIDILLSGMVSYVLDLQESYLLRNVK 240

Query: 241 RGVQRESGKSYIIDVKKGRMRIRMKVALALGVVVLCIGLGMGVMHFVENLGWLDSFYLSV 300
           RGV+RESGKSYIIDVKKGRMRIRMKVALALGVV+LCIG+GMGVMHFVENLGWLDSFYLSV
Sbjct: 241 RGVKRESGKSYIIDVKKGRMRIRMKVALALGVVILCIGVGMGVMHFVENLGWLDSFYLSV 300

Query: 301 MSVTTVGYGDQAFKSMTGRIFASIWLLVSTLAVARAFLYLAEARVDKRHRMMAKWILGQD 360
           MSVTTVGYGDQAFKSMTGRIFASIWLLVSTLAVARAFLYLAEARVDKRHRMMAKWILGQD
Sbjct: 301 MSVTTVGYGDQAFKSMTGRIFASIWLLVSTLAVARAFLYLAEARVDKRHRMMAKWILGQD 360

Query: 361 MTVSEFLAADTDNNGCVSRSEYVLYKLKEMGKVSEKDVMQISNNFDRLDTGNCGKITLAD 420
           MT+SEFLAAD DNNG VSRSEYV+YKLKEMG+VSEKDVMQISNNFDRLD+G CGKITLAD
Sbjct: 361 MTISEFLAADIDNNGFVSRSEYVIYKLKEMGRVSEKDVMQISNNFDRLDSGKCGKITLAD 420

Query: 421 LLESHH 427
           LLESHH
Sbjct: 421 LLESHH 425

BLAST of Carg15395 vs. ExPASy Swiss-Prot
Match: Q9SVV6 (Two-pore potassium channel 3 OS=Arabidopsis thaliana OX=3702 GN=TPK3 PE=2 SV=1)

HSP 1 Score: 512.7 bits (1319), Expect = 4.0e-144
Identity = 282/429 (65.73%), Postives = 343/429 (79.95%), Query Frame = 0

Query: 4   EPLLPYM-SPRGKPLPPPPQLLCPLPENDE--ITLPMTPTEFKDRLIFGP-SSSPQDTSP 63
           +PLL YM SPR   L  PPQLL PLPE++E  I +PMTP+EFK+RLIFGP S SP+D+S 
Sbjct: 7   DPLLQYMISPR---LKKPPQLLFPLPEDNEVAIPMPMTPSEFKERLIFGPFSCSPRDSSH 66

Query: 64  IFDALTL-SLSSSRPSASSSLQDPST--PLAPKSQSPSQQAWLVDSDYSWHKSN-LHRSK 123
             D++   S SSS  + ++   D ST  PL P    P  + WL D   S  + + LHRSK
Sbjct: 67  FIDSMKQPSPSSSSTAVNNPFSDSSTLDPLLP-PPPPQPEPWLSDQTSSHCQGHALHRSK 126

Query: 124 TAPAMAVINDVNHS-QEPKPQFGKQSIIRQAVVLLILYLSLGVLIYWINRDKFSAHETHP 183
           TAPAMAVIND++H  ++  P    +S++RQA  LL++YLSLGVLIYW+NRD +  ++THP
Sbjct: 127 TAPAMAVINDLHHPIRQKDPTETSRSVVRQAFALLVVYLSLGVLIYWLNRDHYVVNQTHP 186

Query: 184 VVDALYFCIVTMCTIGYGDITPNSAATKLFSVLFVLVGFGFIDILLSGMVSYVLDLQESY 243
           VVD LYFCIVTMCTIGYGDITPNS  TKLFS++FVLVGFGFIDILLSGMVSYVLDLQESY
Sbjct: 187 VVDGLYFCIVTMCTIGYGDITPNSVVTKLFSIMFVLVGFGFIDILLSGMVSYVLDLQESY 246

Query: 244 LLRNVKRGVQRESGKSYIIDVKKGRMRIRMKVALALGVVVLCIGLGMGVMHFVENLGWLD 303
           +L + KR  + E  +SYIIDVKKGRMRIR+KVALALGVVVLCI +G+G+MHF+E +GWLD
Sbjct: 247 MLDSAKRRDEPEKRRSYIIDVKKGRMRIRLKVALALGVVVLCIAVGVGIMHFIEEIGWLD 306

Query: 304 SFYLSVMSVTTVGYGDQAFKSMTGRIFASIWLLVSTLAVARAFLYLAEARVDKRHRMMAK 363
           SFYLSVMSVTTVGYGD+AFK++ GR+FA+IWLLVSTLAVARAFLYLAEARVDKR+R  AK
Sbjct: 307 SFYLSVMSVTTVGYGDRAFKTLPGRLFAAIWLLVSTLAVARAFLYLAEARVDKRNRERAK 366

Query: 364 WILGQDMTVSEFLAADTDNNGCVSRSEYVLYKLKEMGKVSEKDVMQISNNFDRLDTGNCG 423
            +L + M+VS+F AAD DNNGCVS++EYV+YKLKEM K+++KD++ IS  FD+LD  + G
Sbjct: 367 KVLCETMSVSQFFAADIDNNGCVSKAEYVIYKLKEMEKITDKDILPISKQFDKLDRCSNG 426

BLAST of Carg15395 vs. ExPASy Swiss-Prot
Match: Q9FL25 (Two-pore potassium channel 2 OS=Arabidopsis thaliana OX=3702 GN=TPK2 PE=2 SV=1)

HSP 1 Score: 456.8 bits (1174), Expect = 2.6e-127
Identity = 254/431 (58.93%), Postives = 326/431 (75.64%), Query Frame = 0

Query: 4   EPLLPY-MSPRGKPLPPPPQLLCPLPENDEITLPM--TPTEFKDRLIFGP-SSSPQDTSP 63
           +PL  Y M+PR   + PPP  L  LPEN+++T+PM  TP E K+RLIFG    S +++S 
Sbjct: 12  DPLRQYLMNPR---INPPPPSLLTLPENNDVTIPMPITPLELKNRLIFGSFVRSRKESSL 71

Query: 64  IFDALTLSLSSSRPSASSSLQDPSTPLAPKSQSPSQQAWLVDSDYSWHKSNLHRSKTAPA 123
             DAL+ + S+S  SA++S  D +  L P ++ P++         S    N HRSKTAPA
Sbjct: 72  PIDALSQNPSTS-SSATTSFSDSTDLLLPLTE-PNKPV-----RKSKPTINFHRSKTAPA 131

Query: 124 MAVINDVNHSQEPK--PQFGKQSIIRQAVVLLILYLSLGVLIYWINRDKFSAHETHPVVD 183
           MA IN+++H  +PK   Q   ++I+ QAV LL++YLSLGVLIYW+NRD ++  +THPVVD
Sbjct: 132 MAAINNISHPNDPKTDQQSDSKTIVNQAVALLVVYLSLGVLIYWLNRDSYNVKQTHPVVD 191

Query: 184 ALYFCIVTMCTIGYGDITPNSAATKLFSVLFVLVGFGFIDILLSGMVSYVLDLQESYLLR 243
           ALYFCIVTMCTIGYGDITP+S  TKLFS+ FVLVGFGF+DILLSGMV+YVLDLQE+Y+L 
Sbjct: 192 ALYFCIVTMCTIGYGDITPDSVVTKLFSIFFVLVGFGFMDILLSGMVTYVLDLQENYMLE 251

Query: 244 NVKRGV----QRESGKSYIIDVKKGRMRIRMKVALALGVVVLCIGLGMGVMHFVENLGWL 303
             +        R+  +SYIIDVKKGRMRIR+KV LALGVVVLC+G G+ +MHFVE +GWL
Sbjct: 252 TARNESLNLNDRDKVRSYIIDVKKGRMRIRLKVGLALGVVVLCLGFGVLIMHFVEKIGWL 311

Query: 304 DSFYLSVMSVTTVGYGDQAFKSMTGRIFASIWLLVSTLAVARAFLYLAEARVDKRHRMMA 363
           DSFY SVMSVTTVGYGD+AF ++ GR+ A++WLLVSTLAVARA L+LAE+RVDKR+R  A
Sbjct: 312 DSFYFSVMSVTTVGYGDRAFNTLAGRLLAAMWLLVSTLAVARAILFLAESRVDKRNRERA 371

Query: 364 KWILGQDMTVSEFLAADTDNNGCVSRSEYVLYKLKEMGKVSEKDVMQISNNFDRLDTGNC 423
           K +LG+ M++S+FL AD D NGCVS++E+V+YKLK+M K++EKD+  I   FD+LD  N 
Sbjct: 372 KKVLGESMSISQFLDADIDCNGCVSKAEFVIYKLKKMDKITEKDINPIGFQFDKLDRTNS 431

Query: 424 GKITLADLLES 425
           G+ITL DLLES
Sbjct: 432 GRITLLDLLES 432

BLAST of Carg15395 vs. ExPASy Swiss-Prot
Match: Q69TN4 (Two pore potassium channel c OS=Oryza sativa subsp. japonica OX=39947 GN=TPKC PE=3 SV=1)

HSP 1 Score: 422.9 bits (1086), Expect = 4.2e-117
Identity = 249/455 (54.73%), Postives = 307/455 (67.47%), Query Frame = 0

Query: 1   MEKEPLLPYMSPRGKPLPPPPQLLCPLPENDEITL---PMTP-----TEFKDRLIFG--- 60
           M+ EPLL        PL P P LL PLPE+ E++    P++P     + +K+R+IFG   
Sbjct: 1   MDTEPLL-------SPLSPSPHLLHPLPEHAEVSTFSPPLSPCPSPASSYKERIIFGAHP 60

Query: 61  ----PSSSPQDTSPIFDALTLSLSSSR---PSASSSLQDPSTPLAPKSQSPSQQAWLVDS 120
               P   P    P        +S      PS SSS   PS    P    PS        
Sbjct: 61  PPPPPPPPPPPPPPRGRRYYRRVSGDDLDVPSCSSSPSPPSDEENPPPNPPSL------F 120

Query: 121 DYSWHKSNLHRSKTAPAMAVINDV--------NHSQEPKPQFGKQSIIRQAVVLLILYLS 180
           D+   ++NLHRS+TAPAMA +N            S+ P P   + +I+  A + L+ YL+
Sbjct: 121 DFIGGRTNLHRSRTAPAMAPLNAAAIAAAAASGDSRNPPPPPRRPAIVLHAFLFLLAYLA 180

Query: 181 LGVLIYWINRDKF--SAHETHPVVDALYFCIVTMCTIGYGDITPNSAATKLFSVLFVLVG 240
           +GV  Y      F  SA  THPV DALYFCIVT+CTIGYGDITP + A KLFS+ FVL+G
Sbjct: 181 MGVTFYAALPGNFTSSAGPTHPVADALYFCIVTLCTIGYGDITPATPAAKLFSISFVLIG 240

Query: 241 FGFIDILLSGMVSYVLDLQESYLLRNVK--RGVQRESGKSYIIDVKKGRMRIRMKVALAL 300
           FGF+DILLSGMVSYVLDLQE  L+  +K  R V R+   +YI D+KKGRMR+RMKVALAL
Sbjct: 241 FGFVDILLSGMVSYVLDLQEHLLITALKNPRSV-RKHRHNYIFDLKKGRMRVRMKVALAL 300

Query: 301 GVVVLCIGLGMGVMHFVENLGWLDSFYLSVMSVTTVGYGDQAFKSMTGRIFASIWLLVST 360
            VV +C+G+G  V+  VENLGWLD+ YL+VMSVTTVGYGD AF+++ GR+FAS WLLVST
Sbjct: 301 TVVAICVGVGAAVLKRVENLGWLDAVYLAVMSVTTVGYGDHAFRTLAGRLFASAWLLVST 360

Query: 361 LAVARAFLYLAEARVDKRHRMMAKWILGQDMTVSEFLAADTDNNGCVSRSEYVLYKLKEM 420
           LAVARAFLYLAE R+DKRHR MA W+L +DMTVSEFLAAD DNNG V++SE+V+YKLKEM
Sbjct: 361 LAVARAFLYLAEMRIDKRHRAMANWVLSRDMTVSEFLAADIDNNGYVTKSEFVVYKLKEM 420

Query: 421 GKVSEKDVMQISNNFDRLDTGNCGKITLADLLESH 426
           GK+SEKD+M I + F R+D+GNCGKITL+DLLESH
Sbjct: 421 GKISEKDIMMICDQFQRMDSGNCGKITLSDLLESH 441

BLAST of Carg15395 vs. ExPASy Swiss-Prot
Match: Q9S6Z8 (Two-pore potassium channel 5 OS=Arabidopsis thaliana OX=3702 GN=TPK5 PE=1 SV=1)

HSP 1 Score: 373.6 bits (958), Expect = 2.9e-102
Identity = 223/422 (52.84%), Postives = 278/422 (65.88%), Query Frame = 0

Query: 18  PPPPQLLCPLPENDEITLPMTPTEFKDRLIFGPSSSPQDTSPIFDALTLSLSSSRPSASS 77
           P P   L P+PEN                   PSSS    S     +T+  S S  S   
Sbjct: 7   PQPRFRLQPIPEN-------------------PSSSSSSAS-----ITIPRSISNTSFFH 66

Query: 78  SLQDPSTPLAPKSQSPSQQAWLVDSDYSWHKSN--------LHRSKTAPAMAVINDVNHS 137
            +      L  +    S Q    D D    ++N        LHRS+TAPAM +I D+   
Sbjct: 67  EISQERLLLHHQDLEQSVQDDKEDQDSDSDETNRFLSQTRPLHRSRTAPAMVIIKDLRTK 126

Query: 138 --QEPKPQFGKQSIIRQAVVLLILYLSLGVLIYWINRDKFSAHETHPVVDALYFCIVTMC 197
             +  KP    +SIIRQA+ LLI+YL+LGV IY  NRD +S  ETHPVVDALYFCIVTMC
Sbjct: 127 PPETKKPSPVSKSIIRQAIFLLIVYLTLGVSIYSFNRDHYSGIETHPVVDALYFCIVTMC 186

Query: 198 TIGYGDITPNSAATKLFSVLFVLVGFGFIDILLSGMVSYVLDLQESYLLRNVK------- 257
           TIGYGDI P +  TK+F+V+FVL GFGF+DILLSG+V+YVLDLQES +L  ++       
Sbjct: 187 TIGYGDIAPLTPWTKIFAVVFVLFGFGFLDILLSGVVNYVLDLQESMILTGIQTRQHHQH 246

Query: 258 RGVQRESGKSYIIDVKKGRMRIRMKVALALGVVVLCIGLGMGVMHFVENLGWLDSFYLSV 317
               R S K YIID +KGRMRIRMKV LAL VVVLCIG+G  V+HFVE LG++DS YLSV
Sbjct: 247 HHHHRFSAKDYIIDFEKGRMRIRMKVCLALCVVVLCIGVGALVLHFVEELGFVDSVYLSV 306

Query: 318 MSVTTVGYGDQAFKSMTGRIFASIWLLVSTLAVARAFLYLAEARVDKRHRMMAKWILGQD 377
           MSVTTVGYGD+AFK++ GR+FA++WLLVSTLAVARAFLYLAEAR+D+RHR   K  L ++
Sbjct: 307 MSVTTVGYGDRAFKTLQGRLFAAVWLLVSTLAVARAFLYLAEARIDRRHRKAVKLALNRE 366

Query: 378 MTVSEFLAADTDNNGCVSRSEYVLYKLKEMGKVSEKDVMQISNNFDRLDTGNCGKITLAD 423
           +TV + L ADT  +G +S+SEY++ KLKEMGK+++KD+ Q+   F++LD    GKITL D
Sbjct: 367 ITVDDLLKADTYQHGFISKSEYIVLKLKEMGKITQKDIDQVVIQFEKLDPNQIGKITLPD 404

BLAST of Carg15395 vs. ExPASy Swiss-Prot
Match: Q8LBL1 (Two-pore potassium channel 1 OS=Arabidopsis thaliana OX=3702 GN=TPK1 PE=1 SV=2)

HSP 1 Score: 219.2 bits (557), Expect = 9.2e-56
Identity = 129/350 (36.86%), Postives = 207/350 (59.14%), Query Frame = 0

Query: 84  TPLAP--KSQSPSQQAWLVDSDYSWHKSNLHRSKTAP-AMAVINDVNHSQEPKPQFGKQS 143
           TPL P  K  + +Q   L     S  K  L RS++AP    + ND     EP P   K  
Sbjct: 8   TPLLPTEKIDTMAQDFNLNSRTSSSRKRRLRRSRSAPRGDCMYNDDVKIDEPPPHPSKIP 67

Query: 144 I-------IRQAVVLLILYLSLGVLIYWINRDKFSAHETHPVVDALYFCIVTMCTIGYGD 203
           +       +R+ ++ L LYL++G L +++ RD+ S H+T  VVDALYFCIVTM T+GYGD
Sbjct: 68  MFSDLNPNLRRVIMFLALYLTIGTLCFYLVRDQISGHKTSGVVDALYFCIVTMTTVGYGD 127

Query: 204 ITPNSAATKLFSVLFVLVGFGFIDILLSGMVSYVLDLQESYLLRNVKRGVQRESGKSYII 263
           + PNS+A++L +  FV  G   +  LLS    Y+++ QE+ L+R     +++  G + I+
Sbjct: 128 LVPNSSASRLLACAFVFSGMVLVGHLLSRAADYLVEKQEALLVRAFH--LRQSFGPTDIL 187

Query: 264 -DVKKGRMRIRMKVALALGVVVLCIGLGMGVMHFVENLGWLDSFYLSVMSVTTVGYGDQA 323
            ++   ++R +      + VV+  +G    VM  VE +  + +FY    +VTT+GYGD++
Sbjct: 188 KELHTNKLRYKCYATCLVLVVLFIVGTIFLVM--VEKMPVISAFYCVCSTVTTLGYGDKS 247

Query: 324 FKSMTGRIFASIWLLVSTLAVARAFLYLAEARVDKRHRMMAKWILGQDMTVSEFLAADTD 383
           F S  GR+FA  W+L S++ +A+ FLY+AE   + + R + KW+L + +T ++  AAD D
Sbjct: 248 FNSEAGRLFAVFWILTSSICLAQFFLYVAELNTENKQRALVKWVLTRRITNNDLEAADLD 307

Query: 384 NNGCVSRSEYVLYKLKEMGKVSEKDVMQISNNFDRLDTGNCGKITLADLL 423
            +G V  +E+++YKLKEMGK+ EKD+  I + F++LD    G +T +D++
Sbjct: 308 EDGVVGAAEFIVYKLKEMGKIDEKDISGIMDEFEQLDYDESGTLTTSDIV 353

BLAST of Carg15395 vs. ExPASy TrEMBL
Match: A0A6J1ED61 (two-pore potassium channel 3-like OS=Cucurbita moschata OX=3662 GN=LOC111433251 PE=3 SV=1)

HSP 1 Score: 827.8 bits (2137), Expect = 2.1e-236
Identity = 423/426 (99.30%), Postives = 426/426 (100.00%), Query Frame = 0

Query: 1   MEKEPLLPYMSPRGKPLPPPPQLLCPLPENDEITLPMTPTEFKDRLIFGPSSSPQDTSPI 60
           MEKEPLLPY+SPRGKPLPPPPQLLCPLPENDEITLPMTPTEFKDRLIFGPSSSPQDTSPI
Sbjct: 1   MEKEPLLPYISPRGKPLPPPPQLLCPLPENDEITLPMTPTEFKDRLIFGPSSSPQDTSPI 60

Query: 61  FDALTLSLSSSRPSASSSLQDPSTPLAPKSQSPSQQAWLVDSDYSWHKSNLHRSKTAPAM 120
           FDALTLSLSSSRPSASSSLQDPSTPLAPKSQSPSQQAWLVDS+YSWHKSNLHRSKTAPAM
Sbjct: 61  FDALTLSLSSSRPSASSSLQDPSTPLAPKSQSPSQQAWLVDSNYSWHKSNLHRSKTAPAM 120

Query: 121 AVINDVNHSQEPKPQFGKQSIIRQAVVLLILYLSLGVLIYWINRDKFSAHETHPVVDALY 180
           AVINDVNHSQEPKPQFGKQSIIRQAVVLLILYLSLGVLIYWINRDKFSAHETHPVVDALY
Sbjct: 121 AVINDVNHSQEPKPQFGKQSIIRQAVVLLILYLSLGVLIYWINRDKFSAHETHPVVDALY 180

Query: 181 FCIVTMCTIGYGDITPNSAATKLFSVLFVLVGFGFIDILLSGMVSYVLDLQESYLLRNVK 240
           FCIVTMCTIGYGDITPNSAATKLFSVLFVLVGFGFIDILLSGMVSYVLDLQESYLLRNVK
Sbjct: 181 FCIVTMCTIGYGDITPNSAATKLFSVLFVLVGFGFIDILLSGMVSYVLDLQESYLLRNVK 240

Query: 241 RGVQRESGKSYIIDVKKGRMRIRMKVALALGVVVLCIGLGMGVMHFVENLGWLDSFYLSV 300
           RGVQRESGKSYIIDVKKGRMRIRMKVALALGVV+LCIGLGMGVMHFVENLGWLDSFYLSV
Sbjct: 241 RGVQRESGKSYIIDVKKGRMRIRMKVALALGVVILCIGLGMGVMHFVENLGWLDSFYLSV 300

Query: 301 MSVTTVGYGDQAFKSMTGRIFASIWLLVSTLAVARAFLYLAEARVDKRHRMMAKWILGQD 360
           MSVTTVGYGDQAFKSMTGRIFASIWLLVSTLAVARAFLYLAEARVDKRHRMMAKWILGQD
Sbjct: 301 MSVTTVGYGDQAFKSMTGRIFASIWLLVSTLAVARAFLYLAEARVDKRHRMMAKWILGQD 360

Query: 361 MTVSEFLAADTDNNGCVSRSEYVLYKLKEMGKVSEKDVMQISNNFDRLDTGNCGKITLAD 420
           MTVSEFLAADTDNNGCVSRSEYVLYKLKEMGKVSEKDVMQISNNFDRLDTGNCGKITLAD
Sbjct: 361 MTVSEFLAADTDNNGCVSRSEYVLYKLKEMGKVSEKDVMQISNNFDRLDTGNCGKITLAD 420

Query: 421 LLESHH 427
           LLESHH
Sbjct: 421 LLESHH 426

BLAST of Carg15395 vs. ExPASy TrEMBL
Match: A0A6J1IME3 (two-pore potassium channel 3-like OS=Cucurbita maxima OX=3661 GN=LOC111478359 PE=3 SV=1)

HSP 1 Score: 826.6 bits (2134), Expect = 4.6e-236
Identity = 423/426 (99.30%), Postives = 425/426 (99.77%), Query Frame = 0

Query: 1   MEKEPLLPYMSPRGKPLPPPPQLLCPLPENDEITLPMTPTEFKDRLIFGPSSSPQDTSPI 60
           MEKEPLLPYMSPRGKPLPPPPQLLCPLPENDEITLPMTPTEFKDRLIFGPSSSPQDTSPI
Sbjct: 1   MEKEPLLPYMSPRGKPLPPPPQLLCPLPENDEITLPMTPTEFKDRLIFGPSSSPQDTSPI 60

Query: 61  FDALTLSLSSSRPSASSSLQDPSTPLAPKSQSPSQQAWLVDSDYSWHKSNLHRSKTAPAM 120
           FDALTLSLSSSRPSASSSLQDPSTPLAPKSQSPSQQAWLVDS+YSWHKSNLHRSKTAPAM
Sbjct: 61  FDALTLSLSSSRPSASSSLQDPSTPLAPKSQSPSQQAWLVDSNYSWHKSNLHRSKTAPAM 120

Query: 121 AVINDVNHSQEPKPQFGKQSIIRQAVVLLILYLSLGVLIYWINRDKFSAHETHPVVDALY 180
           AVINDVNHSQEPKPQFGK SIIRQAVVLLILYLSLGVLIYWINRDKFSAHETHPVVDALY
Sbjct: 121 AVINDVNHSQEPKPQFGKLSIIRQAVVLLILYLSLGVLIYWINRDKFSAHETHPVVDALY 180

Query: 181 FCIVTMCTIGYGDITPNSAATKLFSVLFVLVGFGFIDILLSGMVSYVLDLQESYLLRNVK 240
           FCIVTMCTIGYGDITPNSAATKLFSVLFVLVGFGFIDILLSGMVSYVLDLQESYLLRNVK
Sbjct: 181 FCIVTMCTIGYGDITPNSAATKLFSVLFVLVGFGFIDILLSGMVSYVLDLQESYLLRNVK 240

Query: 241 RGVQRESGKSYIIDVKKGRMRIRMKVALALGVVVLCIGLGMGVMHFVENLGWLDSFYLSV 300
           RGVQRESGKSYIIDVKKGRMRIRMKVALALGVV+LCIGLGMGVMHFVENLGWLDSFYLSV
Sbjct: 241 RGVQRESGKSYIIDVKKGRMRIRMKVALALGVVILCIGLGMGVMHFVENLGWLDSFYLSV 300

Query: 301 MSVTTVGYGDQAFKSMTGRIFASIWLLVSTLAVARAFLYLAEARVDKRHRMMAKWILGQD 360
           MSVTTVGYGDQAFKSMTGRIFASIWLLVSTLAVARAFLYLAEARVDKRHRMMAKWILGQD
Sbjct: 301 MSVTTVGYGDQAFKSMTGRIFASIWLLVSTLAVARAFLYLAEARVDKRHRMMAKWILGQD 360

Query: 361 MTVSEFLAADTDNNGCVSRSEYVLYKLKEMGKVSEKDVMQISNNFDRLDTGNCGKITLAD 420
           MTVSEFLAADTDNNGCVSRSEYVLYKLKEMGKVSEKDVMQISNNFDRLDTGNCGKITLAD
Sbjct: 361 MTVSEFLAADTDNNGCVSRSEYVLYKLKEMGKVSEKDVMQISNNFDRLDTGNCGKITLAD 420

Query: 421 LLESHH 427
           LLESHH
Sbjct: 421 LLESHH 426

BLAST of Carg15395 vs. ExPASy TrEMBL
Match: A0A1S3AYW0 (two-pore potassium channel 3 OS=Cucumis melo OX=3656 GN=LOC103484391 PE=3 SV=1)

HSP 1 Score: 780.8 bits (2015), Expect = 2.9e-222
Identity = 400/426 (93.90%), Postives = 414/426 (97.18%), Query Frame = 0

Query: 1   MEKEPLLPYMSPRGKPLPPPPQLLCPLPENDEITLPMTPTEFKDRLIFGPSSSPQDTSPI 60
           MEKEPLLPY+SPRGKP PPPPQ LCPLPENDEITLPMTPTEFKDRLIFGPSSSPQD SPI
Sbjct: 1   MEKEPLLPYLSPRGKPPPPPPQ-LCPLPENDEITLPMTPTEFKDRLIFGPSSSPQDASPI 60

Query: 61  FDALTLSLSSSRPSASSSLQDPSTPLAPKSQSPSQQAWLVDSDYSWHKSNLHRSKTAPAM 120
           FDALTLSLSSSRPSASSSLQDPSTPL P+SQS SQQAWLVDS+Y+W KSNLHRSKTAPAM
Sbjct: 61  FDALTLSLSSSRPSASSSLQDPSTPLDPRSQSQSQQAWLVDSNYTWPKSNLHRSKTAPAM 120

Query: 121 AVINDVNHSQEPKPQFGKQSIIRQAVVLLILYLSLGVLIYWINRDKFSAHETHPVVDALY 180
           AVINDVNHSQEPKPQFGKQSIIRQAVVLLI+YLSLGVLIYW+NRD F+A ETHPVVDALY
Sbjct: 121 AVINDVNHSQEPKPQFGKQSIIRQAVVLLIVYLSLGVLIYWLNRDNFAAQETHPVVDALY 180

Query: 181 FCIVTMCTIGYGDITPNSAATKLFSVLFVLVGFGFIDILLSGMVSYVLDLQESYLLRNVK 240
           FCIVTMCTIGYGDITPNS ATKLFSVLFVLVGFGFIDILLSGMVSYVLDLQESYLLRNVK
Sbjct: 181 FCIVTMCTIGYGDITPNSTATKLFSVLFVLVGFGFIDILLSGMVSYVLDLQESYLLRNVK 240

Query: 241 RGVQRESGKSYIIDVKKGRMRIRMKVALALGVVVLCIGLGMGVMHFVENLGWLDSFYLSV 300
           RGV+RESGKSYIIDVKKGRMRIRMKVALALGVV+LCIG+GMGVMHFVENLGWLDSFYLSV
Sbjct: 241 RGVKRESGKSYIIDVKKGRMRIRMKVALALGVVILCIGVGMGVMHFVENLGWLDSFYLSV 300

Query: 301 MSVTTVGYGDQAFKSMTGRIFASIWLLVSTLAVARAFLYLAEARVDKRHRMMAKWILGQD 360
           MSVTTVGYGDQAFKSMTGRIFASIWLLVSTLAVARAFLYLAEARVDKRHRMMAKWILGQD
Sbjct: 301 MSVTTVGYGDQAFKSMTGRIFASIWLLVSTLAVARAFLYLAEARVDKRHRMMAKWILGQD 360

Query: 361 MTVSEFLAADTDNNGCVSRSEYVLYKLKEMGKVSEKDVMQISNNFDRLDTGNCGKITLAD 420
           MT+SEFLAAD DNNG VSRSEYV+YKLKEMG+VSEKDVMQISNNFDRLD+G CGKITLAD
Sbjct: 361 MTISEFLAADIDNNGFVSRSEYVIYKLKEMGRVSEKDVMQISNNFDRLDSGKCGKITLAD 420

Query: 421 LLESHH 427
           LLESHH
Sbjct: 421 LLESHH 425

BLAST of Carg15395 vs. ExPASy TrEMBL
Match: A0A6J1CLR2 (two-pore potassium channel 3-like OS=Momordica charantia OX=3673 GN=LOC111012441 PE=3 SV=1)

HSP 1 Score: 763.1 bits (1969), Expect = 6.3e-217
Identity = 395/426 (92.72%), Postives = 406/426 (95.31%), Query Frame = 0

Query: 1   MEKEPLLPYMSPRGKPLPPPPQLLCPLPENDEITLPMTPTEFKDRLIFGPSSSPQDTSPI 60
           MEKEPLLPY SPR K LPPP  LLCPLPENDEITLP+TPTEFKDRLIFGPSSSPQD SPI
Sbjct: 1   MEKEPLLPYQSPRAK-LPPPIPLLCPLPENDEITLPLTPTEFKDRLIFGPSSSPQDASPI 60

Query: 61  FDALTLSLSSSRPSASSSLQDPSTPLAPKSQSPSQQAWLVDSDYSWHKSNLHRSKTAPAM 120
           FDALTLSLSSSR S SSSL DPSTPL PKSQS SQQAWLVDS+Y+W K+NLHRSKTAPAM
Sbjct: 61  FDALTLSLSSSRSSPSSSLYDPSTPLDPKSQSQSQQAWLVDSNYAWPKTNLHRSKTAPAM 120

Query: 121 AVINDVNHSQEPKPQFGKQSIIRQAVVLLILYLSLGVLIYWINRDKFSAHETHPVVDALY 180
           AVINDVNHSQE KPQFG QSIIRQAVVLLI+YLSLGVLIYWIN D F+AHETHPVVDALY
Sbjct: 121 AVINDVNHSQERKPQFGNQSIIRQAVVLLIIYLSLGVLIYWINLDNFTAHETHPVVDALY 180

Query: 181 FCIVTMCTIGYGDITPNSAATKLFSVLFVLVGFGFIDILLSGMVSYVLDLQESYLLRNVK 240
           FCIVTMCTIGYGDITPNS ATKLFSVLFVLVGFGFIDILLSGMVSYVLDLQESYLLRNVK
Sbjct: 181 FCIVTMCTIGYGDITPNSTATKLFSVLFVLVGFGFIDILLSGMVSYVLDLQESYLLRNVK 240

Query: 241 RGVQRESGKSYIIDVKKGRMRIRMKVALALGVVVLCIGLGMGVMHFVENLGWLDSFYLSV 300
           RGVQRESGKSYIIDVKKGRMRIRMKVALALGVV+LCIG+GMGVMHFVE+LGWLDSFYLSV
Sbjct: 241 RGVQRESGKSYIIDVKKGRMRIRMKVALALGVVILCIGVGMGVMHFVEDLGWLDSFYLSV 300

Query: 301 MSVTTVGYGDQAFKSMTGRIFASIWLLVSTLAVARAFLYLAEARVDKRHRMMAKWILGQD 360
           MSVTTVGYGDQAFKS+TGRIFASIWLLVSTLAVARAFLYLAEARVDKRHRMMAKWILGQD
Sbjct: 301 MSVTTVGYGDQAFKSLTGRIFASIWLLVSTLAVARAFLYLAEARVDKRHRMMAKWILGQD 360

Query: 361 MTVSEFLAADTDNNGCVSRSEYVLYKLKEMGKVSEKDVMQISNNFDRLDTGNCGKITLAD 420
           MTVSEFLAAD DNNG VSRSEYV+YKLKEMGKVSEKDVMQISNNFDRLDTG CGKITLAD
Sbjct: 361 MTVSEFLAADIDNNGFVSRSEYVIYKLKEMGKVSEKDVMQISNNFDRLDTGKCGKITLAD 420

Query: 421 LLESHH 427
           LLESHH
Sbjct: 421 LLESHH 425

BLAST of Carg15395 vs. ExPASy TrEMBL
Match: A0A6J1EM72 (two-pore potassium channel 3-like OS=Cucurbita moschata OX=3662 GN=LOC111433827 PE=3 SV=1)

HSP 1 Score: 761.5 bits (1965), Expect = 1.8e-216
Identity = 396/426 (92.96%), Postives = 407/426 (95.54%), Query Frame = 0

Query: 1   MEKEPLLPYMSPRGKPLPPPPQLLCPLPENDEITLPMTPTEFKDRLIFGPSSSPQDTSPI 60
           ME EPLLPYMSPRGKP P  PQL  PLPENDEI LPMTPTEFKDRLIFGPSSSPQD S  
Sbjct: 1   METEPLLPYMSPRGKP-PSLPQLY-PLPENDEIILPMTPTEFKDRLIFGPSSSPQDASSA 60

Query: 61  FDALTLSLSSSRPSASSSLQDPSTPLAPKSQSPSQQAWLVDSDYSWHKSNLHRSKTAPAM 120
           FDALTLSL+S RPSASSS QDPSTPL PKSQSPSQQ WLVDS +S HKSNLHRSKTAPAM
Sbjct: 61  FDALTLSLASPRPSASSSRQDPSTPLDPKSQSPSQQGWLVDSKHSCHKSNLHRSKTAPAM 120

Query: 121 AVINDVNHSQEPKPQFGKQSIIRQAVVLLILYLSLGVLIYWINRDKFSAHETHPVVDALY 180
           AVIND+NHSQEPKPQFGKQSIIRQAVVLLI+YLSLGV+IYWINRD FSA ETHPVVDALY
Sbjct: 121 AVINDINHSQEPKPQFGKQSIIRQAVVLLIIYLSLGVVIYWINRDNFSASETHPVVDALY 180

Query: 181 FCIVTMCTIGYGDITPNSAATKLFSVLFVLVGFGFIDILLSGMVSYVLDLQESYLLRNVK 240
           FCIVTMCTIGYGDITPNSAATKLFSVLFVLVGFGFIDILLSGMVSYVLDLQESYLLRNVK
Sbjct: 181 FCIVTMCTIGYGDITPNSAATKLFSVLFVLVGFGFIDILLSGMVSYVLDLQESYLLRNVK 240

Query: 241 RGVQRESGKSYIIDVKKGRMRIRMKVALALGVVVLCIGLGMGVMHFVENLGWLDSFYLSV 300
           RGVQRESGKSYIIDVKKGRMRIRMKVALALGVV+LCIG+GMGVMHFVE+LGWLDSFYLSV
Sbjct: 241 RGVQRESGKSYIIDVKKGRMRIRMKVALALGVVILCIGIGMGVMHFVEDLGWLDSFYLSV 300

Query: 301 MSVTTVGYGDQAFKSMTGRIFASIWLLVSTLAVARAFLYLAEARVDKRHRMMAKWILGQD 360
           MSVTTVGYGDQAFKSMTGRIFASIWLLVSTLAVARAFLYLAEARVDKRHRMMAKWILGQD
Sbjct: 301 MSVTTVGYGDQAFKSMTGRIFASIWLLVSTLAVARAFLYLAEARVDKRHRMMAKWILGQD 360

Query: 361 MTVSEFLAADTDNNGCVSRSEYVLYKLKEMGKVSEKDVMQISNNFDRLDTGNCGKITLAD 420
           MTVSEFLAADTDNNGCVSRSEYV+YKLKEMGKVSEKDVMQISNNFDRLDTG CGKITLAD
Sbjct: 361 MTVSEFLAADTDNNGCVSRSEYVVYKLKEMGKVSEKDVMQISNNFDRLDTGKCGKITLAD 420

Query: 421 LLESHH 427
           LL++HH
Sbjct: 421 LLQNHH 424

BLAST of Carg15395 vs. TAIR 10
Match: AT4G18160.1 (Ca2+ activated outward rectifying K+ channel 6 )

HSP 1 Score: 512.7 bits (1319), Expect = 2.9e-145
Identity = 282/429 (65.73%), Postives = 343/429 (79.95%), Query Frame = 0

Query: 4   EPLLPYM-SPRGKPLPPPPQLLCPLPENDE--ITLPMTPTEFKDRLIFGP-SSSPQDTSP 63
           +PLL YM SPR   L  PPQLL PLPE++E  I +PMTP+EFK+RLIFGP S SP+D+S 
Sbjct: 7   DPLLQYMISPR---LKKPPQLLFPLPEDNEVAIPMPMTPSEFKERLIFGPFSCSPRDSSH 66

Query: 64  IFDALTL-SLSSSRPSASSSLQDPST--PLAPKSQSPSQQAWLVDSDYSWHKSN-LHRSK 123
             D++   S SSS  + ++   D ST  PL P    P  + WL D   S  + + LHRSK
Sbjct: 67  FIDSMKQPSPSSSSTAVNNPFSDSSTLDPLLP-PPPPQPEPWLSDQTSSHCQGHALHRSK 126

Query: 124 TAPAMAVINDVNHS-QEPKPQFGKQSIIRQAVVLLILYLSLGVLIYWINRDKFSAHETHP 183
           TAPAMAVIND++H  ++  P    +S++RQA  LL++YLSLGVLIYW+NRD +  ++THP
Sbjct: 127 TAPAMAVINDLHHPIRQKDPTETSRSVVRQAFALLVVYLSLGVLIYWLNRDHYVVNQTHP 186

Query: 184 VVDALYFCIVTMCTIGYGDITPNSAATKLFSVLFVLVGFGFIDILLSGMVSYVLDLQESY 243
           VVD LYFCIVTMCTIGYGDITPNS  TKLFS++FVLVGFGFIDILLSGMVSYVLDLQESY
Sbjct: 187 VVDGLYFCIVTMCTIGYGDITPNSVVTKLFSIMFVLVGFGFIDILLSGMVSYVLDLQESY 246

Query: 244 LLRNVKRGVQRESGKSYIIDVKKGRMRIRMKVALALGVVVLCIGLGMGVMHFVENLGWLD 303
           +L + KR  + E  +SYIIDVKKGRMRIR+KVALALGVVVLCI +G+G+MHF+E +GWLD
Sbjct: 247 MLDSAKRRDEPEKRRSYIIDVKKGRMRIRLKVALALGVVVLCIAVGVGIMHFIEEIGWLD 306

Query: 304 SFYLSVMSVTTVGYGDQAFKSMTGRIFASIWLLVSTLAVARAFLYLAEARVDKRHRMMAK 363
           SFYLSVMSVTTVGYGD+AFK++ GR+FA+IWLLVSTLAVARAFLYLAEARVDKR+R  AK
Sbjct: 307 SFYLSVMSVTTVGYGDRAFKTLPGRLFAAIWLLVSTLAVARAFLYLAEARVDKRNRERAK 366

Query: 364 WILGQDMTVSEFLAADTDNNGCVSRSEYVLYKLKEMGKVSEKDVMQISNNFDRLDTGNCG 423
            +L + M+VS+F AAD DNNGCVS++EYV+YKLKEM K+++KD++ IS  FD+LD  + G
Sbjct: 367 KVLCETMSVSQFFAADIDNNGCVSKAEYVIYKLKEMEKITDKDILPISKQFDKLDRCSNG 426

BLAST of Carg15395 vs. TAIR 10
Match: AT5G46370.1 (Ca2+ activated outward rectifying K+ channel 2 )

HSP 1 Score: 456.8 bits (1174), Expect = 1.9e-128
Identity = 254/431 (58.93%), Postives = 326/431 (75.64%), Query Frame = 0

Query: 4   EPLLPY-MSPRGKPLPPPPQLLCPLPENDEITLPM--TPTEFKDRLIFGP-SSSPQDTSP 63
           +PL  Y M+PR   + PPP  L  LPEN+++T+PM  TP E K+RLIFG    S +++S 
Sbjct: 12  DPLRQYLMNPR---INPPPPSLLTLPENNDVTIPMPITPLELKNRLIFGSFVRSRKESSL 71

Query: 64  IFDALTLSLSSSRPSASSSLQDPSTPLAPKSQSPSQQAWLVDSDYSWHKSNLHRSKTAPA 123
             DAL+ + S+S  SA++S  D +  L P ++ P++         S    N HRSKTAPA
Sbjct: 72  PIDALSQNPSTS-SSATTSFSDSTDLLLPLTE-PNKPV-----RKSKPTINFHRSKTAPA 131

Query: 124 MAVINDVNHSQEPK--PQFGKQSIIRQAVVLLILYLSLGVLIYWINRDKFSAHETHPVVD 183
           MA IN+++H  +PK   Q   ++I+ QAV LL++YLSLGVLIYW+NRD ++  +THPVVD
Sbjct: 132 MAAINNISHPNDPKTDQQSDSKTIVNQAVALLVVYLSLGVLIYWLNRDSYNVKQTHPVVD 191

Query: 184 ALYFCIVTMCTIGYGDITPNSAATKLFSVLFVLVGFGFIDILLSGMVSYVLDLQESYLLR 243
           ALYFCIVTMCTIGYGDITP+S  TKLFS+ FVLVGFGF+DILLSGMV+YVLDLQE+Y+L 
Sbjct: 192 ALYFCIVTMCTIGYGDITPDSVVTKLFSIFFVLVGFGFMDILLSGMVTYVLDLQENYMLE 251

Query: 244 NVKRGV----QRESGKSYIIDVKKGRMRIRMKVALALGVVVLCIGLGMGVMHFVENLGWL 303
             +        R+  +SYIIDVKKGRMRIR+KV LALGVVVLC+G G+ +MHFVE +GWL
Sbjct: 252 TARNESLNLNDRDKVRSYIIDVKKGRMRIRLKVGLALGVVVLCLGFGVLIMHFVEKIGWL 311

Query: 304 DSFYLSVMSVTTVGYGDQAFKSMTGRIFASIWLLVSTLAVARAFLYLAEARVDKRHRMMA 363
           DSFY SVMSVTTVGYGD+AF ++ GR+ A++WLLVSTLAVARA L+LAE+RVDKR+R  A
Sbjct: 312 DSFYFSVMSVTTVGYGDRAFNTLAGRLLAAMWLLVSTLAVARAILFLAESRVDKRNRERA 371

Query: 364 KWILGQDMTVSEFLAADTDNNGCVSRSEYVLYKLKEMGKVSEKDVMQISNNFDRLDTGNC 423
           K +LG+ M++S+FL AD D NGCVS++E+V+YKLK+M K++EKD+  I   FD+LD  N 
Sbjct: 372 KKVLGESMSISQFLDADIDCNGCVSKAEFVIYKLKKMDKITEKDINPIGFQFDKLDRTNS 431

Query: 424 GKITLADLLES 425
           G+ITL DLLES
Sbjct: 432 GRITLLDLLES 432

BLAST of Carg15395 vs. TAIR 10
Match: AT4G01840.1 (Ca2+ activated outward rectifying K+ channel 5 )

HSP 1 Score: 373.6 bits (958), Expect = 2.1e-103
Identity = 223/422 (52.84%), Postives = 278/422 (65.88%), Query Frame = 0

Query: 18  PPPPQLLCPLPENDEITLPMTPTEFKDRLIFGPSSSPQDTSPIFDALTLSLSSSRPSASS 77
           P P   L P+PEN                   PSSS    S     +T+  S S  S   
Sbjct: 7   PQPRFRLQPIPEN-------------------PSSSSSSAS-----ITIPRSISNTSFFH 66

Query: 78  SLQDPSTPLAPKSQSPSQQAWLVDSDYSWHKSN--------LHRSKTAPAMAVINDVNHS 137
            +      L  +    S Q    D D    ++N        LHRS+TAPAM +I D+   
Sbjct: 67  EISQERLLLHHQDLEQSVQDDKEDQDSDSDETNRFLSQTRPLHRSRTAPAMVIIKDLRTK 126

Query: 138 --QEPKPQFGKQSIIRQAVVLLILYLSLGVLIYWINRDKFSAHETHPVVDALYFCIVTMC 197
             +  KP    +SIIRQA+ LLI+YL+LGV IY  NRD +S  ETHPVVDALYFCIVTMC
Sbjct: 127 PPETKKPSPVSKSIIRQAIFLLIVYLTLGVSIYSFNRDHYSGIETHPVVDALYFCIVTMC 186

Query: 198 TIGYGDITPNSAATKLFSVLFVLVGFGFIDILLSGMVSYVLDLQESYLLRNVK------- 257
           TIGYGDI P +  TK+F+V+FVL GFGF+DILLSG+V+YVLDLQES +L  ++       
Sbjct: 187 TIGYGDIAPLTPWTKIFAVVFVLFGFGFLDILLSGVVNYVLDLQESMILTGIQTRQHHQH 246

Query: 258 RGVQRESGKSYIIDVKKGRMRIRMKVALALGVVVLCIGLGMGVMHFVENLGWLDSFYLSV 317
               R S K YIID +KGRMRIRMKV LAL VVVLCIG+G  V+HFVE LG++DS YLSV
Sbjct: 247 HHHHRFSAKDYIIDFEKGRMRIRMKVCLALCVVVLCIGVGALVLHFVEELGFVDSVYLSV 306

Query: 318 MSVTTVGYGDQAFKSMTGRIFASIWLLVSTLAVARAFLYLAEARVDKRHRMMAKWILGQD 377
           MSVTTVGYGD+AFK++ GR+FA++WLLVSTLAVARAFLYLAEAR+D+RHR   K  L ++
Sbjct: 307 MSVTTVGYGDRAFKTLQGRLFAAVWLLVSTLAVARAFLYLAEARIDRRHRKAVKLALNRE 366

Query: 378 MTVSEFLAADTDNNGCVSRSEYVLYKLKEMGKVSEKDVMQISNNFDRLDTGNCGKITLAD 423
           +TV + L ADT  +G +S+SEY++ KLKEMGK+++KD+ Q+   F++LD    GKITL D
Sbjct: 367 ITVDDLLKADTYQHGFISKSEYIVLKLKEMGKITQKDIDQVVIQFEKLDPNQIGKITLPD 404

BLAST of Carg15395 vs. TAIR 10
Match: AT5G55630.1 (Outward rectifying potassium channel protein )

HSP 1 Score: 219.2 bits (557), Expect = 6.5e-57
Identity = 129/350 (36.86%), Postives = 207/350 (59.14%), Query Frame = 0

Query: 84  TPLAP--KSQSPSQQAWLVDSDYSWHKSNLHRSKTAP-AMAVINDVNHSQEPKPQFGKQS 143
           TPL P  K  + +Q   L     S  K  L RS++AP    + ND     EP P   K  
Sbjct: 8   TPLLPTEKIDTMAQDFNLNSRTSSSRKRRLRRSRSAPRGDCMYNDDVKIDEPPPHPSKIP 67

Query: 144 I-------IRQAVVLLILYLSLGVLIYWINRDKFSAHETHPVVDALYFCIVTMCTIGYGD 203
           +       +R+ ++ L LYL++G L +++ RD+ S H+T  VVDALYFCIVTM T+GYGD
Sbjct: 68  MFSDLNPNLRRVIMFLALYLTIGTLCFYLVRDQISGHKTSGVVDALYFCIVTMTTVGYGD 127

Query: 204 ITPNSAATKLFSVLFVLVGFGFIDILLSGMVSYVLDLQESYLLRNVKRGVQRESGKSYII 263
           + PNS+A++L +  FV  G   +  LLS    Y+++ QE+ L+R     +++  G + I+
Sbjct: 128 LVPNSSASRLLACAFVFSGMVLVGHLLSRAADYLVEKQEALLVRAFH--LRQSFGPTDIL 187

Query: 264 -DVKKGRMRIRMKVALALGVVVLCIGLGMGVMHFVENLGWLDSFYLSVMSVTTVGYGDQA 323
            ++   ++R +      + VV+  +G    VM  VE +  + +FY    +VTT+GYGD++
Sbjct: 188 KELHTNKLRYKCYATCLVLVVLFIVGTIFLVM--VEKMPVISAFYCVCSTVTTLGYGDKS 247

Query: 324 FKSMTGRIFASIWLLVSTLAVARAFLYLAEARVDKRHRMMAKWILGQDMTVSEFLAADTD 383
           F S  GR+FA  W+L S++ +A+ FLY+AE   + + R + KW+L + +T ++  AAD D
Sbjct: 248 FNSEAGRLFAVFWILTSSICLAQFFLYVAELNTENKQRALVKWVLTRRITNNDLEAADLD 307

Query: 384 NNGCVSRSEYVLYKLKEMGKVSEKDVMQISNNFDRLDTGNCGKITLADLL 423
            +G V  +E+++YKLKEMGK+ EKD+  I + F++LD    G +T +D++
Sbjct: 308 EDGVVGAAEFIVYKLKEMGKIDEKDISGIMDEFEQLDYDESGTLTTSDIV 353

BLAST of Carg15395 vs. TAIR 10
Match: AT5G55630.2 (Outward rectifying potassium channel protein )

HSP 1 Score: 219.2 bits (557), Expect = 6.5e-57
Identity = 129/350 (36.86%), Postives = 207/350 (59.14%), Query Frame = 0

Query: 84  TPLAP--KSQSPSQQAWLVDSDYSWHKSNLHRSKTAP-AMAVINDVNHSQEPKPQFGKQS 143
           TPL P  K  + +Q   L     S  K  L RS++AP    + ND     EP P   K  
Sbjct: 8   TPLLPTEKIDTMAQDFNLNSRTSSSRKRRLRRSRSAPRGDCMYNDDVKIDEPPPHPSKIP 67

Query: 144 I-------IRQAVVLLILYLSLGVLIYWINRDKFSAHETHPVVDALYFCIVTMCTIGYGD 203
           +       +R+ ++ L LYL++G L +++ RD+ S H+T  VVDALYFCIVTM T+GYGD
Sbjct: 68  MFSDLNPNLRRVIMFLALYLTIGTLCFYLVRDQISGHKTSGVVDALYFCIVTMTTVGYGD 127

Query: 204 ITPNSAATKLFSVLFVLVGFGFIDILLSGMVSYVLDLQESYLLRNVKRGVQRESGKSYII 263
           + PNS+A++L +  FV  G   +  LLS    Y+++ QE+ L+R     +++  G + I+
Sbjct: 128 LVPNSSASRLLACAFVFSGMVLVGHLLSRAADYLVEKQEALLVRAFH--LRQSFGPTDIL 187

Query: 264 -DVKKGRMRIRMKVALALGVVVLCIGLGMGVMHFVENLGWLDSFYLSVMSVTTVGYGDQA 323
            ++   ++R +      + VV+  +G    VM  VE +  + +FY    +VTT+GYGD++
Sbjct: 188 KELHTNKLRYKCYATCLVLVVLFIVGTIFLVM--VEKMPVISAFYCVCSTVTTLGYGDKS 247

Query: 324 FKSMTGRIFASIWLLVSTLAVARAFLYLAEARVDKRHRMMAKWILGQDMTVSEFLAADTD 383
           F S  GR+FA  W+L S++ +A+ FLY+AE   + + R + KW+L + +T ++  AAD D
Sbjct: 248 FNSEAGRLFAVFWILTSSICLAQFFLYVAELNTENKQRALVKWVLTRRITNNDLEAADLD 307

Query: 384 NNGCVSRSEYVLYKLKEMGKVSEKDVMQISNNFDRLDTGNCGKITLADLL 423
            +G V  +E+++YKLKEMGK+ EKD+  I + F++LD    G +T +D++
Sbjct: 308 EDGVVGAAEFIVYKLKEMGKIDEKDISGIMDEFEQLDYDESGTLTTSDIV 353

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
KAG6581409.13.0e-237100.00Two-pore potassium channel 3, partial [Cucurbita argyrosperma subsp. sororia] >K... [more]
XP_023544618.11.5e-23699.53two-pore potassium channel 3-like [Cucurbita pepo subsp. pepo][more]
XP_022926002.14.3e-23699.30two-pore potassium channel 3-like [Cucurbita moschata][more]
XP_022978331.19.6e-23699.30two-pore potassium channel 3-like [Cucurbita maxima][more]
XP_008439665.16.0e-22293.90PREDICTED: two-pore potassium channel 3 [Cucumis melo][more]
Match NameE-valueIdentityDescription
Q9SVV64.0e-14465.73Two-pore potassium channel 3 OS=Arabidopsis thaliana OX=3702 GN=TPK3 PE=2 SV=1[more]
Q9FL252.6e-12758.93Two-pore potassium channel 2 OS=Arabidopsis thaliana OX=3702 GN=TPK2 PE=2 SV=1[more]
Q69TN44.2e-11754.73Two pore potassium channel c OS=Oryza sativa subsp. japonica OX=39947 GN=TPKC PE... [more]
Q9S6Z82.9e-10252.84Two-pore potassium channel 5 OS=Arabidopsis thaliana OX=3702 GN=TPK5 PE=1 SV=1[more]
Q8LBL19.2e-5636.86Two-pore potassium channel 1 OS=Arabidopsis thaliana OX=3702 GN=TPK1 PE=1 SV=2[more]
Match NameE-valueIdentityDescription
A0A6J1ED612.1e-23699.30two-pore potassium channel 3-like OS=Cucurbita moschata OX=3662 GN=LOC111433251 ... [more]
A0A6J1IME34.6e-23699.30two-pore potassium channel 3-like OS=Cucurbita maxima OX=3661 GN=LOC111478359 PE... [more]
A0A1S3AYW02.9e-22293.90two-pore potassium channel 3 OS=Cucumis melo OX=3656 GN=LOC103484391 PE=3 SV=1[more]
A0A6J1CLR26.3e-21792.72two-pore potassium channel 3-like OS=Momordica charantia OX=3673 GN=LOC111012441... [more]
A0A6J1EM721.8e-21692.96two-pore potassium channel 3-like OS=Cucurbita moschata OX=3662 GN=LOC111433827 ... [more]
Match NameE-valueIdentityDescription
AT4G18160.12.9e-14565.73Ca2+ activated outward rectifying K+ channel 6 [more]
AT5G46370.11.9e-12858.93Ca2+ activated outward rectifying K+ channel 2 [more]
AT4G01840.12.1e-10352.84Ca2+ activated outward rectifying K+ channel 5 [more]
AT5G55630.16.5e-5736.86Outward rectifying potassium channel protein [more]
AT5G55630.26.5e-5736.86Outward rectifying potassium channel protein [more]
InterPro
Analysis Name: InterPro Annotations of Silver-seed gourd (SMH-JMG-627) v2
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR003280Two pore domain potassium channelPRINTSPR013332POREKCHANELcoord: 304..313
score: 47.04
coord: 184..212
score: 35.57
IPR003280Two pore domain potassium channelPANTHERPTHR11003POTASSIUM CHANNEL, SUBFAMILY Kcoord: 91..421
NoneNo IPR availableGENE3D1.10.287.70coord: 271..334
e-value: 9.5E-14
score: 53.2
NoneNo IPR availableGENE3D1.10.287.70coord: 138..238
e-value: 2.9E-21
score: 77.1
NoneNo IPR availableGENE3D1.10.238.10coord: 335..426
e-value: 5.1E-6
score: 28.4
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 1..33
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 72..94
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 11..26
NoneNo IPR availablePANTHERPTHR11003:SF282TWO-PORE POTASSIUM CHANNEL 3coord: 91..421
NoneNo IPR availableSUPERFAMILY81324Voltage-gated potassium channelscoord: 256..362
NoneNo IPR availableSUPERFAMILY81324Voltage-gated potassium channelscoord: 141..227
IPR013099Potassium channel domainPFAMPF07885Ion_trans_2coord: 148..227
e-value: 2.6E-16
score: 59.3
coord: 272..341
e-value: 4.3E-11
score: 42.6
IPR018247EF-Hand 1, calcium-binding sitePROSITEPS00018EF_HAND_1coord: 370..382
IPR011992EF-hand domain pairSUPERFAMILY47473EF-handcoord: 363..423

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Carg15395-RACarg15395-RAmRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0071805 potassium ion transmembrane transport
biological_process GO:0030322 stabilization of membrane potential
cellular_component GO:0005887 integral component of plasma membrane
cellular_component GO:0009705 plant-type vacuole membrane
cellular_component GO:0016020 membrane
molecular_function GO:0022841 potassium ion leak channel activity
molecular_function GO:0005267 potassium channel activity