Carg15369 (gene) Silver-seed gourd (SMH-JMG-627) v2

Overview
NameCarg15369
Typegene
OrganismCucurbita argyrosperma subsp. argyrosperma cv. SMH-JMG-627 (Silver-seed gourd (SMH-JMG-627) v2)
Descriptionprotein NRT1/ PTR FAMILY 4.5-like
LocationCarg_Chr03: 9467141 .. 9469928 (+)
RNA-Seq ExpressionCarg15369
SyntenyCarg15369
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRCDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
CCTCCAATAATTTCTAGATTTGAATAAATAAAACTTTCAACCATGTCACGTCTCTAATTGAGTGATTAACATTAATATAATAAATGATTAAGATACTCATAGTGAAACAAAATCAACTTTTCTATGAGACTATATCCTATTGGAAAAAAAGTTCACCACATATTTCTATGAATGGAATAACTCACTATCATAACACAACTGTAAGGATTCTCTAAGGTCGATAACTGATATAATAGTGAAGCAGAGGCATGCAGTTATGAACACGCTATCAGAGGTCACACTATTCTTACCATGCAACAAGAAACCGAAAGCTATCAGTTCATGACAGCAATGGAGGATCCTGGCCAGTTCCAATAGTATGTTTGATGTTCTTCATTTGAGCTCCAATTAAGTTCCAATCAAGGAGGTTAGGTCCTTCAGCTGATCTCGTGAGTTGCGAGGTTTATCTGCTAGTTCATTTCTATGGAGGAGATGCTTGAGGTAGTTGAAGGAAAAGTGGACTGGAAGGGAAGATCAGCTTTCAAACAAAAACATGGAGGAACAAAATCTTCTTTGCTCGCACTAGGTACGAAATCATTTACTCATCAACGTTTTTAGATGTAGTGAGCCAAAGTGTAACCGCCCAAGGCCAAAACTAGCCACATATATTGTCTTCTTTGAGCTTTCCCTTTCAGGCTTCCCTCAAAGTTTTAAAACGTGTCTATTAAAAAGAGGTTTTTACACCCTTTTAAAAGAATGTTTCGTTTCTCTCTCCAATCGAGATAGGATCTCACAGAGACAGAACGCGCTCTTCTCTTTAGCAGGCTACATAACCACATGCACCCTTGTATGCTCATTGTAATCACAACACAGTTTCTTGTACCTTCTGGGTTTTCTGTTGCAGTGACATATGGTTTCGATCAAATGGCAAACTTCGCGCTTGCAGTGAACTTGACTACGTATTTCAATACTGTGATGCACTTGGAACTAGCGGATGCGGCCAACCAACTGACGAATTACATGGGAACATGTTACATTCTCTCTATTCCAATGGCTGTTCTCGCAGACACATGCTTGGGCAGAGTCAAAACTGTCATCATTTCTGGCTGTCTTGAGTTTCTGGTCAGCCCAACTTTAGTGGCTTTACTTTGCTGCCCCTACCGATTCCCATATGCTAAACACTGTTACTGTTATTTTAGGCGCTAATATTGCTCATGATACAAGCTCACTACCCGAAGCTCAAGCCACCACTTTGCAATATATTTGATAAACAATCCCACTGCGAGACAGTTGGAGGAGGAAATGCTGCCCTCCTCTATGTTCCTCTATATGCATTAGCTATTGGGAATGCAGGCATTAAGGCTGCATTACCACCGCATGGAGCTGATCAATTTGATGAAAAAGACCCCAAAGAGGCGATGCAAATATCCAGCTTCTTCAATCAACTGTTGCTAGGATCGTCCCTCGGTATAGCTGCTAGTTTAACTCTTGTTGTGTGGATCCAAGACAACAAAGGTTGGGACTGGGGTTTCGGGGTATCTTCTGCAGCCATCTTGTTCGGTATAGTCATGTTTGTTGCCGGATTGCCACTGTACAGAATGCACATTATTTCTGGGTCTAATGCAATCGTCCAAGTTTTACAGGTATGTCCGTGAGACAAGCAAAATACAGAGTTGGAGGCAAATATATTGTTACTTCTGTCCAAATAAATGTTGTTTTTAGGTCTATGTTGCAGCCACTCGTAATAGAAATCTTGTCCTTCCCGAAGACCCTGCAGATCTTTATGAGATTGAAAGGGACAAAGAGGCTGTTATGGAAGAAGACTTTCTGCCTCATAGAAACATTTGCAGGTTTTGTACATGAAACTCTGTTTAAACTTTCTAGTTTGACAGCCCCATAAGCATTGAATACCATGATAACTCGAATATACAGGTTTCTAGACAAAGCGGCAATTCAACAAACACCTTGTAGGCAAGTTGAAACTCCCGAAGCTTCAAGCCCTTGGAAACTATGCACAGTGACCCAAGTAGAGAATGCCAAAGTAATTCTCAGCATGATACCAATTTTCTGTTGCACAGTGAATATGAGCCTCTGCTTTGCCCAGCTCCAAACCTTCTCCATCCAACAGGGCCTCACCATGGACACAAAACTCACACCCTCCTTCAAAATCCCCCCGGCGTCACTCACCATCATCCCAGTCTTCTTTCTCATCATCATGGCGCCAATCTACGACAAGATTCTTGTCCCCTTTGCTCGAAAATTCACAGGCATCCCCACAGGAATCACCCCCTTGCAACGAGTAGGTGTTGGATTGGTTCTGTCCATCATCTCCATGGCCGTCGCGGCACTAGTGGAAGTGAAGCGTAAAGGCGTTGCGAGAGACCACAACATGTTGGATGCAACCCCTGTTTTGCAGCCATTGCCAATCAGCACGTTCTGGCTATCATTTCAATTCTTCATATTCGGAATCGCAGATCTCTTTGCATACGTAGGGCTTCTCGATTTCTTCTACTCCGAGGCGCCAAAAGCCCTAAAATCTGTCTCCACTTGCTTCCTTTGGAGTTCGATGGGCATGGGATACTTCTTGAGCACCATAATTGTGAAGGTTGTGAACAGTGCTACGAAAGGGATTACGAAAAATGGAGGTTGGCTGATTGGAAACAACATTAATCGGAACCATTTGAATCTCTTCTACTGGCTGCTTTCAATCTTGAGCTTCATCAACTTCTTTATCTATATATTTGCTACCCAGAAATACAATTACAGAAACCATAAACCAGCCATCTCCGCCATTGATGACAGAAGGTAG

mRNA sequence

CCTCCAATAATTTCTAGATTTGAATAAATAAAACTTTCAACCATGTCACGTCTCTAATTGAGTGATTAACATTAATATAATAAATGATTAAGATACTCATAGTGAAACAAAATCAACTTTTCTATGAGACTATATCCTATTGGAAAAAAAGTTCACCACATATTTCTATGAATGGAATAACTCACTATCATAACACAACTGTAAGGATTCTCTAAGGTCGATAACTGATATAATAGTGAAGCAGAGGCATGCAGTTATGAACACGCTATCAGAGGTCACACTATTCTTACCATGCAACAAGAAACCGAAAGCTATCAGTTCATGACAGCAATGGAGGATCCTGGCCAGTTCCAATAGTATGTTTGATGTTCTTCATTTGAGCTCCAATTAAGTTCCAATCAAGGAGGTTAGGTCCTTCAGCTGATCTCGTGAGTTGCGAGGTTTATCTGCTAGTTCATTTCTATGGAGGAGATGCTTGAGGTAGTTGAAGGAAAAGTGGACTGGAAGGGAAGATCAGCTTTCAAACAAAAACATGGAGGAACAAAATCTTCTTTGCTCGCACTAGTGACATATGGTTTCGATCAAATGGCAAACTTCGCGCTTGCAGTGAACTTGACTACGTATTTCAATACTGTGATGCACTTGGAACTAGCGGATGCGGCCAACCAACTGACGAATTACATGGGAACATGTTACATTCTCTCTATTCCAATGGCTGTTCTCGCAGACACATGCTTGGGCAGAGTCAAAACTGTCATCATTTCTGGCTGTCTTGAGTTTCTGGCGCTAATATTGCTCATGATACAAGCTCACTACCCGAAGCTCAAGCCACCACTTTGCAATATATTTGATAAACAATCCCACTGCGAGACAGTTGGAGGAGGAAATGCTGCCCTCCTCTATGTTCCTCTATATGCATTAGCTATTGGGAATGCAGGCATTAAGGCTGCATTACCACCGCATGGAGCTGATCAATTTGATGAAAAAGACCCCAAAGAGGCGATGCAAATATCCAGCTTCTTCAATCAACTGTTGCTAGGATCGTCCCTCGGTATAGCTGCTAGTTTAACTCTTGTTGTGTGGATCCAAGACAACAAAGGTTGGGACTGGGGTTTCGGGGTATCTTCTGCAGCCATCTTGTTCGGTATAGTCATGTTTGTTGCCGGATTGCCACTGTACAGAATGCACATTATTTCTGGGTCTAATGCAATCGTCCAAGTTTTACAGGTCTATGTTGCAGCCACTCGTAATAGAAATCTTGTCCTTCCCGAAGACCCTGCAGATCTTTATGAGATTGAAAGGGACAAAGAGGCTGTTATGGAAGAAGACTTTCTGCCTCATAGAAACATTTGCAGGTTTCTAGACAAAGCGGCAATTCAACAAACACCTTGTAGGCAAGTTGAAACTCCCGAAGCTTCAAGCCCTTGGAAACTATGCACAGTGACCCAAGTAGAGAATGCCAAAGTAATTCTCAGCATGATACCAATTTTCTGTTGCACAGTGAATATGAGCCTCTGCTTTGCCCAGCTCCAAACCTTCTCCATCCAACAGGGCCTCACCATGGACACAAAACTCACACCCTCCTTCAAAATCCCCCCGGCGTCACTCACCATCATCCCAGTCTTCTTTCTCATCATCATGGCGCCAATCTACGACAAGATTCTTGTCCCCTTTGCTCGAAAATTCACAGGCATCCCCACAGGAATCACCCCCTTGCAACGAGTAGGTGTTGGATTGGTTCTGTCCATCATCTCCATGGCCGTCGCGGCACTAGTGGAAGTGAAGCGTAAAGGCGTTGCGAGAGACCACAACATGTTGGATGCAACCCCTGTTTTGCAGCCATTGCCAATCAGCACGTTCTGGCTATCATTTCAATTCTTCATATTCGGAATCGCAGATCTCTTTGCATACGTAGGGCTTCTCGATTTCTTCTACTCCGAGGCGCCAAAAGCCCTAAAATCTGTCTCCACTTGCTTCCTTTGGAGTTCGATGGGCATGGGATACTTCTTGAGCACCATAATTGTGAAGGTTGTGAACAGTGCTACGAAAGGGATTACGAAAAATGGAGGTTGGCTGATTGGAAACAACATTAATCGGAACCATTTGAATCTCTTCTACTGGCTGCTTTCAATCTTGAGCTTCATCAACTTCTTTATCTATATATTTGCTACCCAGAAATACAATTACAGAAACCATAAACCAGCCATCTCCGCCATTGATGACAGAAGGTAG

Coding sequence (CDS)

ATGGAGGAGATGCTTGAGGTAGTTGAAGGAAAAGTGGACTGGAAGGGAAGATCAGCTTTCAAACAAAAACATGGAGGAACAAAATCTTCTTTGCTCGCACTAGTGACATATGGTTTCGATCAAATGGCAAACTTCGCGCTTGCAGTGAACTTGACTACGTATTTCAATACTGTGATGCACTTGGAACTAGCGGATGCGGCCAACCAACTGACGAATTACATGGGAACATGTTACATTCTCTCTATTCCAATGGCTGTTCTCGCAGACACATGCTTGGGCAGAGTCAAAACTGTCATCATTTCTGGCTGTCTTGAGTTTCTGGCGCTAATATTGCTCATGATACAAGCTCACTACCCGAAGCTCAAGCCACCACTTTGCAATATATTTGATAAACAATCCCACTGCGAGACAGTTGGAGGAGGAAATGCTGCCCTCCTCTATGTTCCTCTATATGCATTAGCTATTGGGAATGCAGGCATTAAGGCTGCATTACCACCGCATGGAGCTGATCAATTTGATGAAAAAGACCCCAAAGAGGCGATGCAAATATCCAGCTTCTTCAATCAACTGTTGCTAGGATCGTCCCTCGGTATAGCTGCTAGTTTAACTCTTGTTGTGTGGATCCAAGACAACAAAGGTTGGGACTGGGGTTTCGGGGTATCTTCTGCAGCCATCTTGTTCGGTATAGTCATGTTTGTTGCCGGATTGCCACTGTACAGAATGCACATTATTTCTGGGTCTAATGCAATCGTCCAAGTTTTACAGGTCTATGTTGCAGCCACTCGTAATAGAAATCTTGTCCTTCCCGAAGACCCTGCAGATCTTTATGAGATTGAAAGGGACAAAGAGGCTGTTATGGAAGAAGACTTTCTGCCTCATAGAAACATTTGCAGGTTTCTAGACAAAGCGGCAATTCAACAAACACCTTGTAGGCAAGTTGAAACTCCCGAAGCTTCAAGCCCTTGGAAACTATGCACAGTGACCCAAGTAGAGAATGCCAAAGTAATTCTCAGCATGATACCAATTTTCTGTTGCACAGTGAATATGAGCCTCTGCTTTGCCCAGCTCCAAACCTTCTCCATCCAACAGGGCCTCACCATGGACACAAAACTCACACCCTCCTTCAAAATCCCCCCGGCGTCACTCACCATCATCCCAGTCTTCTTTCTCATCATCATGGCGCCAATCTACGACAAGATTCTTGTCCCCTTTGCTCGAAAATTCACAGGCATCCCCACAGGAATCACCCCCTTGCAACGAGTAGGTGTTGGATTGGTTCTGTCCATCATCTCCATGGCCGTCGCGGCACTAGTGGAAGTGAAGCGTAAAGGCGTTGCGAGAGACCACAACATGTTGGATGCAACCCCTGTTTTGCAGCCATTGCCAATCAGCACGTTCTGGCTATCATTTCAATTCTTCATATTCGGAATCGCAGATCTCTTTGCATACGTAGGGCTTCTCGATTTCTTCTACTCCGAGGCGCCAAAAGCCCTAAAATCTGTCTCCACTTGCTTCCTTTGGAGTTCGATGGGCATGGGATACTTCTTGAGCACCATAATTGTGAAGGTTGTGAACAGTGCTACGAAAGGGATTACGAAAAATGGAGGTTGGCTGATTGGAAACAACATTAATCGGAACCATTTGAATCTCTTCTACTGGCTGCTTTCAATCTTGAGCTTCATCAACTTCTTTATCTATATATTTGCTACCCAGAAATACAATTACAGAAACCATAAACCAGCCATCTCCGCCATTGATGACAGAAGGTAG

Protein sequence

MEEMLEVVEGKVDWKGRSAFKQKHGGTKSSLLALVTYGFDQMANFALAVNLTTYFNTVMHLELADAANQLTNYMGTCYILSIPMAVLADTCLGRVKTVIISGCLEFLALILLMIQAHYPKLKPPLCNIFDKQSHCETVGGGNAALLYVPLYALAIGNAGIKAALPPHGADQFDEKDPKEAMQISSFFNQLLLGSSLGIAASLTLVVWIQDNKGWDWGFGVSSAAILFGIVMFVAGLPLYRMHIISGSNAIVQVLQVYVAATRNRNLVLPEDPADLYEIERDKEAVMEEDFLPHRNICRFLDKAAIQQTPCRQVETPEASSPWKLCTVTQVENAKVILSMIPIFCCTVNMSLCFAQLQTFSIQQGLTMDTKLTPSFKIPPASLTIIPVFFLIIMAPIYDKILVPFARKFTGIPTGITPLQRVGVGLVLSIISMAVAALVEVKRKGVARDHNMLDATPVLQPLPISTFWLSFQFFIFGIADLFAYVGLLDFFYSEAPKALKSVSTCFLWSSMGMGYFLSTIIVKVVNSATKGITKNGGWLIGNNINRNHLNLFYWLLSILSFINFFIYIFATQKYNYRNHKPAISAIDDRR
Homology
BLAST of Carg15369 vs. NCBI nr
Match: KAG6581385.1 (Protein NRT1/ PTR FAMILY 4.6, partial [Cucurbita argyrosperma subsp. sororia] >KAG7034669.1 Protein NRT1/ PTR FAMILY 4.6 [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 1156.7 bits (2991), Expect = 0.0e+00
Identity = 589/589 (100.00%), Postives = 589/589 (100.00%), Query Frame = 0

Query: 1   MEEMLEVVEGKVDWKGRSAFKQKHGGTKSSLLALVTYGFDQMANFALAVNLTTYFNTVMH 60
           MEEMLEVVEGKVDWKGRSAFKQKHGGTKSSLLALVTYGFDQMANFALAVNLTTYFNTVMH
Sbjct: 1   MEEMLEVVEGKVDWKGRSAFKQKHGGTKSSLLALVTYGFDQMANFALAVNLTTYFNTVMH 60

Query: 61  LELADAANQLTNYMGTCYILSIPMAVLADTCLGRVKTVIISGCLEFLALILLMIQAHYPK 120
           LELADAANQLTNYMGTCYILSIPMAVLADTCLGRVKTVIISGCLEFLALILLMIQAHYPK
Sbjct: 61  LELADAANQLTNYMGTCYILSIPMAVLADTCLGRVKTVIISGCLEFLALILLMIQAHYPK 120

Query: 121 LKPPLCNIFDKQSHCETVGGGNAALLYVPLYALAIGNAGIKAALPPHGADQFDEKDPKEA 180
           LKPPLCNIFDKQSHCETVGGGNAALLYVPLYALAIGNAGIKAALPPHGADQFDEKDPKEA
Sbjct: 121 LKPPLCNIFDKQSHCETVGGGNAALLYVPLYALAIGNAGIKAALPPHGADQFDEKDPKEA 180

Query: 181 MQISSFFNQLLLGSSLGIAASLTLVVWIQDNKGWDWGFGVSSAAILFGIVMFVAGLPLYR 240
           MQISSFFNQLLLGSSLGIAASLTLVVWIQDNKGWDWGFGVSSAAILFGIVMFVAGLPLYR
Sbjct: 181 MQISSFFNQLLLGSSLGIAASLTLVVWIQDNKGWDWGFGVSSAAILFGIVMFVAGLPLYR 240

Query: 241 MHIISGSNAIVQVLQVYVAATRNRNLVLPEDPADLYEIERDKEAVMEEDFLPHRNICRFL 300
           MHIISGSNAIVQVLQVYVAATRNRNLVLPEDPADLYEIERDKEAVMEEDFLPHRNICRFL
Sbjct: 241 MHIISGSNAIVQVLQVYVAATRNRNLVLPEDPADLYEIERDKEAVMEEDFLPHRNICRFL 300

Query: 301 DKAAIQQTPCRQVETPEASSPWKLCTVTQVENAKVILSMIPIFCCTVNMSLCFAQLQTFS 360
           DKAAIQQTPCRQVETPEASSPWKLCTVTQVENAKVILSMIPIFCCTVNMSLCFAQLQTFS
Sbjct: 301 DKAAIQQTPCRQVETPEASSPWKLCTVTQVENAKVILSMIPIFCCTVNMSLCFAQLQTFS 360

Query: 361 IQQGLTMDTKLTPSFKIPPASLTIIPVFFLIIMAPIYDKILVPFARKFTGIPTGITPLQR 420
           IQQGLTMDTKLTPSFKIPPASLTIIPVFFLIIMAPIYDKILVPFARKFTGIPTGITPLQR
Sbjct: 361 IQQGLTMDTKLTPSFKIPPASLTIIPVFFLIIMAPIYDKILVPFARKFTGIPTGITPLQR 420

Query: 421 VGVGLVLSIISMAVAALVEVKRKGVARDHNMLDATPVLQPLPISTFWLSFQFFIFGIADL 480
           VGVGLVLSIISMAVAALVEVKRKGVARDHNMLDATPVLQPLPISTFWLSFQFFIFGIADL
Sbjct: 421 VGVGLVLSIISMAVAALVEVKRKGVARDHNMLDATPVLQPLPISTFWLSFQFFIFGIADL 480

Query: 481 FAYVGLLDFFYSEAPKALKSVSTCFLWSSMGMGYFLSTIIVKVVNSATKGITKNGGWLIG 540
           FAYVGLLDFFYSEAPKALKSVSTCFLWSSMGMGYFLSTIIVKVVNSATKGITKNGGWLIG
Sbjct: 481 FAYVGLLDFFYSEAPKALKSVSTCFLWSSMGMGYFLSTIIVKVVNSATKGITKNGGWLIG 540

Query: 541 NNINRNHLNLFYWLLSILSFINFFIYIFATQKYNYRNHKPAISAIDDRR 590
           NNINRNHLNLFYWLLSILSFINFFIYIFATQKYNYRNHKPAISAIDDRR
Sbjct: 541 NNINRNHLNLFYWLLSILSFINFFIYIFATQKYNYRNHKPAISAIDDRR 589

BLAST of Carg15369 vs. NCBI nr
Match: XP_022925822.1 (protein NRT1/ PTR FAMILY 4.6-like [Cucurbita moschata])

HSP 1 Score: 1154.4 bits (2985), Expect = 0.0e+00
Identity = 588/589 (99.83%), Postives = 588/589 (99.83%), Query Frame = 0

Query: 1   MEEMLEVVEGKVDWKGRSAFKQKHGGTKSSLLALVTYGFDQMANFALAVNLTTYFNTVMH 60
           MEEMLEVVEGKVDWKGRSAFKQKHGGTKSSLLALVTYGFDQMANFALAVNLTTYFNTVMH
Sbjct: 1   MEEMLEVVEGKVDWKGRSAFKQKHGGTKSSLLALVTYGFDQMANFALAVNLTTYFNTVMH 60

Query: 61  LELADAANQLTNYMGTCYILSIPMAVLADTCLGRVKTVIISGCLEFLALILLMIQAHYPK 120
           LELADAANQLTNYMGTCYILSIPMAVLADTCLGRVKTVIISGCLEFLALILLMIQAHYPK
Sbjct: 61  LELADAANQLTNYMGTCYILSIPMAVLADTCLGRVKTVIISGCLEFLALILLMIQAHYPK 120

Query: 121 LKPPLCNIFDKQSHCETVGGGNAALLYVPLYALAIGNAGIKAALPPHGADQFDEKDPKEA 180
           LKPPLCNIFDKQSHCETVGGGNAALLYVPLYALAIGNAGIKAALPPHGADQFDEKDPKEA
Sbjct: 121 LKPPLCNIFDKQSHCETVGGGNAALLYVPLYALAIGNAGIKAALPPHGADQFDEKDPKEA 180

Query: 181 MQISSFFNQLLLGSSLGIAASLTLVVWIQDNKGWDWGFGVSSAAILFGIVMFVAGLPLYR 240
           MQISSFFNQLLLGSSLGIAASLTLVVWIQDNKGWDWGFGVSSAAILFGIVMFVAGLPLYR
Sbjct: 181 MQISSFFNQLLLGSSLGIAASLTLVVWIQDNKGWDWGFGVSSAAILFGIVMFVAGLPLYR 240

Query: 241 MHIISGSNAIVQVLQVYVAATRNRNLVLPEDPADLYEIERDKEAVMEEDFLPHRNICRFL 300
           MHIISGSNAIVQVLQVYVAATRNRNLVLPEDPADLYEIERDKEAVMEEDFLPHRNICRFL
Sbjct: 241 MHIISGSNAIVQVLQVYVAATRNRNLVLPEDPADLYEIERDKEAVMEEDFLPHRNICRFL 300

Query: 301 DKAAIQQTPCRQVETPEASSPWKLCTVTQVENAKVILSMIPIFCCTVNMSLCFAQLQTFS 360
           DKAAIQQTPCRQVETPEASSPWKLCTVTQVENAKVILSMIPIFCCTVNMSLCFAQLQTFS
Sbjct: 301 DKAAIQQTPCRQVETPEASSPWKLCTVTQVENAKVILSMIPIFCCTVNMSLCFAQLQTFS 360

Query: 361 IQQGLTMDTKLTPSFKIPPASLTIIPVFFLIIMAPIYDKILVPFARKFTGIPTGITPLQR 420
           IQQGLTMDTKLTPSFKIPPASLTIIPVFFLIIMAPIYDKILVPFARKFTGIPTGITPLQR
Sbjct: 361 IQQGLTMDTKLTPSFKIPPASLTIIPVFFLIIMAPIYDKILVPFARKFTGIPTGITPLQR 420

Query: 421 VGVGLVLSIISMAVAALVEVKRKGVARDHNMLDATPVLQPLPISTFWLSFQFFIFGIADL 480
           VGVGLVLSIISMAVAALVEVKRKGVARDHNMLDATPVLQPLPISTFWLSFQFFIFGIADL
Sbjct: 421 VGVGLVLSIISMAVAALVEVKRKGVARDHNMLDATPVLQPLPISTFWLSFQFFIFGIADL 480

Query: 481 FAYVGLLDFFYSEAPKALKSVSTCFLWSSMGMGYFLSTIIVKVVNSATKGITKNGGWLIG 540
           FAYVGLLDFFYSEAPKALKSVSTCFLWSSMGMGYFLSTIIVKVVNSATKGITKNGGWLIG
Sbjct: 481 FAYVGLLDFFYSEAPKALKSVSTCFLWSSMGMGYFLSTIIVKVVNSATKGITKNGGWLIG 540

Query: 541 NNINRNHLNLFYWLLSILSFINFFIYIFATQKYNYRNHKPAISAIDDRR 590
           NNINRNHLNLFYWLLSILSFINFFIYIFATQKYNYRNHKPAISAIDD R
Sbjct: 541 NNINRNHLNLFYWLLSILSFINFFIYIFATQKYNYRNHKPAISAIDDSR 589

BLAST of Carg15369 vs. NCBI nr
Match: XP_023543943.1 (protein NRT1/ PTR FAMILY 4.6-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 1143.6 bits (2957), Expect = 0.0e+00
Identity = 581/589 (98.64%), Postives = 584/589 (99.15%), Query Frame = 0

Query: 1   MEEMLEVVEGKVDWKGRSAFKQKHGGTKSSLLALVTYGFDQMANFALAVNLTTYFNTVMH 60
           MEEMLEVVEGKVDWKGRSAFKQKHGGTKSSLLALVTYGFDQMANFALAVNLTTYFNTVMH
Sbjct: 1   MEEMLEVVEGKVDWKGRSAFKQKHGGTKSSLLALVTYGFDQMANFALAVNLTTYFNTVMH 60

Query: 61  LELADAANQLTNYMGTCYILSIPMAVLADTCLGRVKTVIISGCLEFLALILLMIQAHYPK 120
           LELADAANQLTNYMGTCYILSIPMAVLADTCLGRVKTVIISGCLEFLALILLMIQAHYPK
Sbjct: 61  LELADAANQLTNYMGTCYILSIPMAVLADTCLGRVKTVIISGCLEFLALILLMIQAHYPK 120

Query: 121 LKPPLCNIFDKQSHCETVGGGNAALLYVPLYALAIGNAGIKAALPPHGADQFDEKDPKEA 180
           LKPPLCNIFDKQSHCETVGG  AALLYVPLYALAIGNAGIKAALPPHGADQFDEKDPKEA
Sbjct: 121 LKPPLCNIFDKQSHCETVGGAKAALLYVPLYALAIGNAGIKAALPPHGADQFDEKDPKEA 180

Query: 181 MQISSFFNQLLLGSSLGIAASLTLVVWIQDNKGWDWGFGVSSAAILFGIVMFVAGLPLYR 240
           MQISSFFNQLLLGSSLGIAASLTLVVWIQDNKGWDWGFGVSSAAILFGIVMFVAGLPLYR
Sbjct: 181 MQISSFFNQLLLGSSLGIAASLTLVVWIQDNKGWDWGFGVSSAAILFGIVMFVAGLPLYR 240

Query: 241 MHIISGSNAIVQVLQVYVAATRNRNLVLPEDPADLYEIERDKEAVMEEDFLPHRNICRFL 300
           MHIISGSNAIVQVLQVYVAATRNRNLVLPEDPADLYEIERDKEAVMEEDFLPHRNICRFL
Sbjct: 241 MHIISGSNAIVQVLQVYVAATRNRNLVLPEDPADLYEIERDKEAVMEEDFLPHRNICRFL 300

Query: 301 DKAAIQQTPCRQVETPEASSPWKLCTVTQVENAKVILSMIPIFCCTVNMSLCFAQLQTFS 360
           DKAAIQQTPCRQV+TPEASSPWKLCTVTQVENAKVILSMIPIFCCTVNMSLCFAQLQTFS
Sbjct: 301 DKAAIQQTPCRQVDTPEASSPWKLCTVTQVENAKVILSMIPIFCCTVNMSLCFAQLQTFS 360

Query: 361 IQQGLTMDTKLTPSFKIPPASLTIIPVFFLIIMAPIYDKILVPFARKFTGIPTGITPLQR 420
           IQQGLTMDTKLTPSF IPP SLTIIPVFFLI+MAPIYDKILVPFARKFTGIPTGITPLQR
Sbjct: 361 IQQGLTMDTKLTPSFNIPPPSLTIIPVFFLILMAPIYDKILVPFARKFTGIPTGITPLQR 420

Query: 421 VGVGLVLSIISMAVAALVEVKRKGVARDHNMLDATPVLQPLPISTFWLSFQFFIFGIADL 480
           VGVGLVLSIISMAVAALVEVKRKGVARDHNMLDATPVLQPLPISTFWLSFQFFIFGIADL
Sbjct: 421 VGVGLVLSIISMAVAALVEVKRKGVARDHNMLDATPVLQPLPISTFWLSFQFFIFGIADL 480

Query: 481 FAYVGLLDFFYSEAPKALKSVSTCFLWSSMGMGYFLSTIIVKVVNSATKGITKNGGWLIG 540
           FAYVGLLDFFYSEAPKALKSVSTCFLWSSMGMGYFLSTIIVK+VNSATKGITKNGGWLIG
Sbjct: 481 FAYVGLLDFFYSEAPKALKSVSTCFLWSSMGMGYFLSTIIVKIVNSATKGITKNGGWLIG 540

Query: 541 NNINRNHLNLFYWLLSILSFINFFIYIFATQKYNYRNHKPAISAIDDRR 590
           NNINRNHLNLFYWLLSILSFINFFIYIFATQKYNYRNHKPAISAIDD R
Sbjct: 541 NNINRNHLNLFYWLLSILSFINFFIYIFATQKYNYRNHKPAISAIDDSR 589

BLAST of Carg15369 vs. NCBI nr
Match: XP_022978676.1 (protein NRT1/ PTR FAMILY 4.6-like isoform X1 [Cucurbita maxima])

HSP 1 Score: 1115.5 bits (2884), Expect = 0.0e+00
Identity = 570/589 (96.77%), Postives = 576/589 (97.79%), Query Frame = 0

Query: 1   MEEMLEVVEGKVDWKGRSAFKQKHGGTKSSLLALVTYGFDQMANFALAVNLTTYFNTVMH 60
           MEEMLEVVEGKVDWKGRSAFKQKHGGTKSSLL LVTYGFDQMANFALAVNLTTYFNTVMH
Sbjct: 1   MEEMLEVVEGKVDWKGRSAFKQKHGGTKSSLLTLVTYGFDQMANFALAVNLTTYFNTVMH 60

Query: 61  LELADAANQLTNYMGTCYILSIPMAVLADTCLGRVKTVIISGCLEFLALILLMIQAHYPK 120
           LELADAANQLTNYMGTCYILSIPMAVLADTCLGRVKTVIISGCLEFLALILLMIQAHYPK
Sbjct: 61  LELADAANQLTNYMGTCYILSIPMAVLADTCLGRVKTVIISGCLEFLALILLMIQAHYPK 120

Query: 121 LKPPLCNIFDKQSHCETVGGGNAALLYVPLYALAIGNAGIKAALPPHGADQFDEKDPKEA 180
           LKPPLCNIFDKQSHCETVGGGNAALLYVPLYALAIGNAGIKAALPPHGADQFDEKDPKEA
Sbjct: 121 LKPPLCNIFDKQSHCETVGGGNAALLYVPLYALAIGNAGIKAALPPHGADQFDEKDPKEA 180

Query: 181 MQISSFFNQLLLGSSLGIAASLTLVVWIQDNKGWDWGFGVSSAAILFGIVMFVAGLPLYR 240
           MQISSFFNQLLLGSSLGIAASLTLVVWIQD+KGWDWGFGVSSAAILFG+VMFVAGLP YR
Sbjct: 181 MQISSFFNQLLLGSSLGIAASLTLVVWIQDDKGWDWGFGVSSAAILFGMVMFVAGLPRYR 240

Query: 241 MHIISGSNAIVQVLQVYVAATRNRNLVLPEDPADLYEIERDKEAVMEEDFLPHRNICRFL 300
           MHIISGSNAIVQVLQVYVAATRNRNLVLPEDPADLYEIERDKEAVMEEDFLPHRNI RFL
Sbjct: 241 MHIISGSNAIVQVLQVYVAATRNRNLVLPEDPADLYEIERDKEAVMEEDFLPHRNIFRFL 300

Query: 301 DKAAIQQTPCRQVETPEASSPWKLCTVTQVENAKVILSMIPIFCCTVNMSLCFAQLQTFS 360
           DKAAIQQTPCRQVE PEASSPWKLCTVTQVENAKVILSMIPIFCCTVNMSLCFAQLQTFS
Sbjct: 301 DKAAIQQTPCRQVEIPEASSPWKLCTVTQVENAKVILSMIPIFCCTVNMSLCFAQLQTFS 360

Query: 361 IQQGLTMDTKLTPSFKIPPASLTIIPVFFLIIMAPIYDKILVPFARKFTGIPTGITPLQR 420
           IQQGLTMDTKLTPSF IPP SLTIIPVFFLI+MAPIYDKILVPFARK TGIPTGITPLQR
Sbjct: 361 IQQGLTMDTKLTPSFNIPPPSLTIIPVFFLILMAPIYDKILVPFARKLTGIPTGITPLQR 420

Query: 421 VGVGLVLSIISMAVAALVEVKRKGVARDHNMLDATPVLQPLPISTFWLSFQFFIFGIADL 480
           VGVGLVLSIISMAVAALVEVKRKGVARDHNMLDATPVLQPLPISTFWLSFQFFIFGIADL
Sbjct: 421 VGVGLVLSIISMAVAALVEVKRKGVARDHNMLDATPVLQPLPISTFWLSFQFFIFGIADL 480

Query: 481 FAYVGLLDFFYSEAPKALKSVSTCFLWSSMGMGYFLSTIIVKVVNSATKGITKNGGWLIG 540
           FAYVGLL+FFYSEAPKALKSVSTCFLWSSMGMGYFLSTIIVK VNSATKGIT+NGGWLIG
Sbjct: 481 FAYVGLLEFFYSEAPKALKSVSTCFLWSSMGMGYFLSTIIVKTVNSATKGITRNGGWLIG 540

Query: 541 NNINRNHLNLFYWLLSILSFINFFIYIFATQKYNYRNHKPAISAIDDRR 590
           NNINRNHLNLFYWLLSILSFINFFIYIFATQKY+YRNH P   AIDD R
Sbjct: 541 NNINRNHLNLFYWLLSILSFINFFIYIFATQKYSYRNHNP---AIDDSR 586

BLAST of Carg15369 vs. NCBI nr
Match: XP_023522562.1 (protein NRT1/ PTR FAMILY 4.6-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 1062.4 bits (2746), Expect = 1.4e-306
Identity = 540/548 (98.54%), Postives = 543/548 (99.09%), Query Frame = 0

Query: 42  MANFALAVNLTTYFNTVMHLELADAANQLTNYMGTCYILSIPMAVLADTCLGRVKTVIIS 101
           MANFALAVNLTTYFNTVMHLELADAANQLTNYMGTCYILSIPMAVLADTCLGRVKTVIIS
Sbjct: 1   MANFALAVNLTTYFNTVMHLELADAANQLTNYMGTCYILSIPMAVLADTCLGRVKTVIIS 60

Query: 102 GCLEFLALILLMIQAHYPKLKPPLCNIFDKQSHCETVGGGNAALLYVPLYALAIGNAGIK 161
           GCLEFLALILLMIQAHYPKLKPPLCNIFDKQSHCETVGG  AALLYVPLYALAIGNAGIK
Sbjct: 61  GCLEFLALILLMIQAHYPKLKPPLCNIFDKQSHCETVGGAKAALLYVPLYALAIGNAGIK 120

Query: 162 AALPPHGADQFDEKDPKEAMQISSFFNQLLLGSSLGIAASLTLVVWIQDNKGWDWGFGVS 221
           AALPPHGADQFDEKDPKEAMQISSFFNQLLLGSSLGIAASLTLVVWIQDNKGWDWGFGVS
Sbjct: 121 AALPPHGADQFDEKDPKEAMQISSFFNQLLLGSSLGIAASLTLVVWIQDNKGWDWGFGVS 180

Query: 222 SAAILFGIVMFVAGLPLYRMHIISGSNAIVQVLQVYVAATRNRNLVLPEDPADLYEIERD 281
           SAAILFGIVMFVAGLPLYRMHIISGSNAIVQVLQVYVAATRNRNLVLPEDPADLYEIERD
Sbjct: 181 SAAILFGIVMFVAGLPLYRMHIISGSNAIVQVLQVYVAATRNRNLVLPEDPADLYEIERD 240

Query: 282 KEAVMEEDFLPHRNICRFLDKAAIQQTPCRQVETPEASSPWKLCTVTQVENAKVILSMIP 341
           KEAVMEEDFLPHRNICRFLDKAAIQQTPCRQV+TPEASSPWKLCTVTQVENAKVILSMIP
Sbjct: 241 KEAVMEEDFLPHRNICRFLDKAAIQQTPCRQVDTPEASSPWKLCTVTQVENAKVILSMIP 300

Query: 342 IFCCTVNMSLCFAQLQTFSIQQGLTMDTKLTPSFKIPPASLTIIPVFFLIIMAPIYDKIL 401
           IFCCTVNMSLCFAQLQTFSIQQGLTMDTKLTPSF IPP SLTIIPVFFLI+MAPIYDKIL
Sbjct: 301 IFCCTVNMSLCFAQLQTFSIQQGLTMDTKLTPSFNIPPPSLTIIPVFFLILMAPIYDKIL 360

Query: 402 VPFARKFTGIPTGITPLQRVGVGLVLSIISMAVAALVEVKRKGVARDHNMLDATPVLQPL 461
           VPFARKFTGIPTGITPLQRVGVGLVLSIISMAVAALVEVKRKGVARDHNMLDATPVLQPL
Sbjct: 361 VPFARKFTGIPTGITPLQRVGVGLVLSIISMAVAALVEVKRKGVARDHNMLDATPVLQPL 420

Query: 462 PISTFWLSFQFFIFGIADLFAYVGLLDFFYSEAPKALKSVSTCFLWSSMGMGYFLSTIIV 521
           PISTFWLSFQFFIFGIADLFAYVGLLDFFYSEAPKALKSVSTCFLWSSMGMGYFLSTIIV
Sbjct: 421 PISTFWLSFQFFIFGIADLFAYVGLLDFFYSEAPKALKSVSTCFLWSSMGMGYFLSTIIV 480

Query: 522 KVVNSATKGITKNGGWLIGNNINRNHLNLFYWLLSILSFINFFIYIFATQKYNYRNHKPA 581
           K+VNSATKGITKNGGWLIGNNINRNHLNLFYWLLSILSFINFFIYIFATQKYNYRNHKPA
Sbjct: 481 KIVNSATKGITKNGGWLIGNNINRNHLNLFYWLLSILSFINFFIYIFATQKYNYRNHKPA 540

Query: 582 ISAIDDRR 590
           ISAIDD R
Sbjct: 541 ISAIDDSR 548

BLAST of Carg15369 vs. ExPASy Swiss-Prot
Match: Q8H157 (Protein NRT1/ PTR FAMILY 4.6 OS=Arabidopsis thaliana OX=3702 GN=NPF4.6 PE=1 SV=1)

HSP 1 Score: 448.7 bits (1153), Expect = 9.9e-125
Identity = 256/598 (42.81%), Postives = 370/598 (61.87%), Query Frame = 0

Query: 1   MEEMLEVVEGKVDWKGRSAFKQKHGGTKSSLLALVTYGFDQMANFALAVNLTTYFNTVMH 60
           +EE +   EG  DW+ R+A K +HGG  ++   LV    + +A  A A NL  Y    MH
Sbjct: 3   VEEEVSRWEGYADWRNRAAVKGRHGGMLAASFVLVVEILENLAYLANASNLVLYLREYMH 62

Query: 61  LELADAANQLTNYMGTCYILSIPMAVLADTCLGRVKTVIISGCLEFLALILLMIQAHYPK 120
           +  + +AN +TN+MGT ++L++    L+D      +  +IS  +EFL LI+L IQA  P 
Sbjct: 63  MSPSKSANDVTNFMGTAFLLALLGGFLSDAFFSTFQIFLISASIEFLGLIILTIQARTPS 122

Query: 121 LKPPLCNIFDKQSHCETVGGGNAALLYVPLYALAIGNAGIKAALPPHGADQFDEKDPKEA 180
           L PP C+       CE V G  AA+L+V LY +A+G  GIK +L  HGA+QFDE  PK  
Sbjct: 123 LMPPSCD----SPTCEEVSGSKAAMLFVGLYLVALGVGGIKGSLASHGAEQFDESTPKGR 182

Query: 181 MQISSFFNQLLLGSSLGIAASLTLVVWIQDNKGWDWGFGVSSAAILFGIVMFVAGLPLYR 240
            Q S+FFN  +   + G   ++T VVW++DNKGW+WGFGVS+ AI   I++F++G   YR
Sbjct: 183 KQRSTFFNYFVFCLACGALVAVTFVVWLEDNKGWEWGFGVSTIAIFVSILIFLSGSRFYR 242

Query: 241 MHIISGSNAIVQVLQVYVAA---------TRNRNLVLPEDPAD--------LYEIERDKE 300
             I  GS  +  +L+V +AA         + N    +   P++          E + + E
Sbjct: 243 NKIPCGS-PLTTILKVLLAASVKCCSSGSSSNAVASMSVSPSNHCVSKGKKEVESQGELE 302

Query: 301 AVMEEDFLPHR----NICRFLDKAAIQQTPCRQVETPEASSPWKLCTVTQVENAKVILSM 360
              +E+ LP R    N  + L+ AA ++   R +E          CTV QVE+ K++L M
Sbjct: 303 KPRQEEALPPRAQLTNSLKVLNGAADEKPVHRLLE----------CTVQQVEDVKIVLKM 362

Query: 361 IPIFCCTVNMSLCFAQLQTFSIQQGLTMDTKLTPSFKIPPASLTIIPVFFLIIMAPIYDK 420
           +PIF CT+ ++ C AQL TFS+QQ  +M+TK+  S KIPPASL I PV F++I+APIYD 
Sbjct: 363 LPIFACTIMLNCCLAQLSTFSVQQAASMNTKI-GSLKIPPASLPIFPVVFIMILAPIYDH 422

Query: 421 ILVPFARKFTGIPTGITPLQRVGVGLVLSIISMAVAALVEVKRKGVARDHNMLDATPVLQ 480
           +++PFARK T   TG+T LQR+GVGLVLSI++MAVAALVE+KRKGVA+D  +LD+    +
Sbjct: 423 LIIPFARKATKTETGVTHLQRIGVGLVLSILAMAVAALVEIKRKGVAKDSGLLDSK---E 482

Query: 481 PLPISTFWLSFQFFIFGIADLFAYVGLLDFFYSEAPKALKSVSTCFLWSSMGMGYFLSTI 540
            LP++  W++ Q+   G ADLF   GLL++F++EAP +++S++T   W+S+ MGY+LS++
Sbjct: 483 TLPVTFLWIALQYLFLGSADLFTLAGLLEYFFTEAPSSMRSLATSLSWASLAMGYYLSSV 542

Query: 541 IVKVVNSATKGITKNGGWLIGNNINRNHLNLFYWLLSILSFINFFIYIFATQKYNYRN 578
           IV +VNS T G + N  WL G +INR  L+ FYWL+ +LS  NF  Y+F   +Y YR+
Sbjct: 543 IVSIVNSIT-GSSGNTPWLRGKSINRYKLDYFYWLMCVLSAANFLHYLFWAMRYKYRS 580

BLAST of Carg15369 vs. ExPASy Swiss-Prot
Match: Q8VYE4 (Protein NRT1/ PTR FAMILY 4.5 OS=Arabidopsis thaliana OX=3702 GN=NPF4.5 PE=2 SV=1)

HSP 1 Score: 420.6 bits (1080), Expect = 2.9e-116
Identity = 236/568 (41.55%), Postives = 348/568 (61.27%), Query Frame = 0

Query: 9   EGKVDWKGRSAFKQKHGGTKSSLLALVTYGFDQMANFALAVNLTTYFNTVMHLELADAAN 68
           EG  DW+ ++A + +HGG  ++   L     + +A  A A NL  Y    MH+ LA +++
Sbjct: 13  EGYADWRNKAALRGRHGGMLAASFVLAVEILENLAFLANASNLVLYLKNFMHMSLARSSS 72

Query: 69  QLTNYMGTCYILSIPMAVLADTCLGRVKTVIISGCLEFLALILLMIQAHYPKLKPPLCNI 128
           ++T +M T ++L++    LAD         +IS  +EFL LILL IQA  P L PP C  
Sbjct: 73  EVTTFMATAFLLALLGGFLADAFFSTFVIFLISASIEFLGLILLTIQARRPSLMPPPCK- 132

Query: 129 FDKQSHCETVGGGNAALLYVPLYALAIGNAGIKAALPPHGADQFDEKDPKEAMQISSFFN 188
                 CE VGG  AA L+V LY +++G  GIK +LP HGA+QFDE  PK   Q S+FFN
Sbjct: 133 SSAALRCEVVGGSKAAFLFVGLYLVSLGIGGIKGSLPSHGAEQFDEGTPKGRKQRSTFFN 192

Query: 189 QLLLGSSLGIAASLTLVVWIQDNKGWDWGFGVSSAAILFGIVMFVAGLPLYRMHIISGSN 248
             +   S G   ++T VVWI+DNKGW+WGFGVS+ +I   I++F+ G   Y+  I  GS 
Sbjct: 193 YYVFCLSCGALVAVTFVVWIEDNKGWEWGFGVSTISIFLSILVFLLGSRFYKNKIPRGS- 252

Query: 249 AIVQVLQVYVAAT-RNRNLVLPEDPADLYEIERDKEAVMEEDFLPHRNICRFLDKAAIQQ 308
            +  + +V +AA+  + +     +     E++ + E       L +   C  L+KA   +
Sbjct: 253 PLTTIFKVLLAASIVSCSSKTSSNHFTSREVQSEHEEKTPSQSLTNSLTC--LNKAIEGK 312

Query: 309 TPCRQVETPEASSPWKLCTVTQVENAKVILSMIPIFCCTVNMSLCFAQLQTFSIQQGLTM 368
           T             W  CTV QVE+ K++L M+PIF CT+ ++ C AQL T+S+ Q  TM
Sbjct: 313 T----------HHIWLECTVQQVEDVKIVLKMLPIFGCTIMLNCCLAQLSTYSVHQAATM 372

Query: 369 DTKLTPSFKIPPASLTIIPVFFLIIMAPIYDKILVPFARKFTGIPTGITPLQRVGVGLVL 428
           + K+  +F +P ASL + PV F++I+AP YD +++PFARK T    GIT LQR+GVGLVL
Sbjct: 373 NRKIV-NFNVPSASLPVFPVVFMLILAPTYDHLIIPFARKVTKSEIGITHLQRIGVGLVL 432

Query: 429 SIISMAVAALVEVKRKGVARDHNMLDATPVLQPLPISTFWLSFQFFIFGIADLFAYVGLL 488
           SI++MAVAALVE+KRK VAR+  +LD+    + LPI+  W++ Q+   G ADLF   GLL
Sbjct: 433 SIVAMAVAALVELKRKQVAREAGLLDSE---ETLPITFLWIALQYLFLGSADLFTLAGLL 492

Query: 489 DFFYSEAPKALKSVSTCFLWSSMGMGYFLSTIIVKVVNSATKGITKNGGWLIGNNINRNH 548
           +FF++EAP +++S++T   W+S+ +GY+LS+++V +VN  TK   ++  WL G  +NRN 
Sbjct: 493 EFFFTEAPSSMRSLATSLSWASLALGYYLSSVMVPIVNRVTKSAGQS-PWL-GEKLNRNR 552

Query: 549 LNLFYWLLSILSFINFFIYIFATQKYNY 576
           L+LFYWL+ +LS +NF  Y+F  ++Y Y
Sbjct: 553 LDLFYWLMCVLSVVNFLHYLFWAKRYKY 560

BLAST of Carg15369 vs. ExPASy Swiss-Prot
Match: Q93VV5 (Protein NRT1/ PTR FAMILY 4.3 OS=Arabidopsis thaliana OX=3702 GN=NPF4.3 PE=2 SV=1)

HSP 1 Score: 419.5 bits (1077), Expect = 6.4e-116
Identity = 237/560 (42.32%), Postives = 337/560 (60.18%), Query Frame = 0

Query: 9   EGKVDWKGRSAFKQKHGGTKSSLLALVTYGFDQMANFALAVNLTTYFNTVMHLELADAAN 68
           E  VDW+GR +   KHGG +++L  L    F+ M   A+  NL TY    MH  L+ AAN
Sbjct: 27  EESVDWRGRPSNPNKHGGMRAALFVLGLQAFEIMGIAAVGNNLITYVINEMHFPLSKAAN 86

Query: 69  QLTNYMGTCYILSIPMAVLADTCLGRVKTVIISGCLEFLALILLMIQAHYPKLKPPLCNI 128
            +TN++GT +I ++    L+D  LG   T+II G +E    ILL +QAH P+LKPP CN 
Sbjct: 87  IVTNFVGTIFIFALLGGYLSDAFLGSFWTIIIFGFVELSGFILLSVQAHLPQLKPPKCNP 146

Query: 129 FDKQSHCETVGGGNAALLYVPLYALAIGNAGIKAALPPHGADQFDEKDPKEAMQISSFFN 188
              Q+ CE   G  A + ++ LY +A+G+  +K  +  HGADQF +  PK++ ++SS+FN
Sbjct: 147 LIDQT-CEEAKGFKAMIFFMALYLVALGSGCVKPNMIAHGADQFSQSHPKQSKRLSSYFN 206

Query: 189 QLLLGSSLGIAASLTLVVWIQDNKGWDWGFGVSSAAILFGIVMFVAGLPLYRMHIISGSN 248
                 S+G   +LTL+VW+Q + G D GFGVS+AA+  GI+  V+G  +Y  +     +
Sbjct: 207 AAYFAFSMGELIALTLLVWVQTHSGMDIGFGVSAAAMTMGIISLVSG-TMYFRNKRPRRS 266

Query: 249 AIVQVLQVYVAATRNRNLVLPEDPADLYEIERDKEAVMEEDFLPHRNICRFLDKAAIQQT 308
               +  V VAA   R L  P DP  L+        V+    LPH    RFLDKA I   
Sbjct: 267 IFTPIAHVIVAAILKRKLASPSDPRMLHGDHHVANDVVPSSTLPHTPRFRFLDKACI--- 326

Query: 309 PCRQVETPEASSPWKLCTVTQVENAKVILSMIPIFCCTVNMSLCFAQLQTFSIQQGLTMD 368
             +  +T    SPW+LCTVTQVE  K ++S++PIF  T+  +   AQLQTFS+QQG +M+
Sbjct: 327 --KIQDTNTKESPWRLCTVTQVEQVKTLISLVPIFASTIVFNTILAQLQTFSVQQGSSMN 386

Query: 369 TKLTPSFKIPPASLTIIPVFFLIIMAPIYDKILVPFARKFTGIPTGITPLQRVGVGLVLS 428
           T+L+ SF IPPASL  IP   LI + P+YD  LVPFARK TG  +GI PL R+G+GL LS
Sbjct: 387 TRLSNSFHIPPASLQAIPYIMLIFLVPLYDSFLVPFARKLTGHNSGIPPLTRIGIGLFLS 446

Query: 429 IISMAVAALVEVKRKGVARDHNMLDATPVLQPLPISTFWLSFQFFIFGIADLFAYVGLLD 488
             SM  AA++E KR    RD ++LD         +S FW++ QF IFGI+++F  VGL++
Sbjct: 447 TFSMVSAAMLEKKR----RDSSVLDGR------ILSIFWITPQFLIFGISEMFTAVGLIE 506

Query: 489 FFYSEAPKALKSVSTCFLWSSMGMGYFLSTIIVKVVNSATKGITKNGGWLIGNNINRNHL 548
           FFY ++ K ++S      + S   G++ S+++V VVN  T     + GWL  N++N++ L
Sbjct: 507 FFYKQSAKGMESFLMALTYCSYSFGFYFSSVLVSVVNKITSTSVDSKGWLGENDLNKDRL 566

Query: 549 NLFYWLLSILSFINFFIYIF 569
           +LFYWLL++LS +NF  Y+F
Sbjct: 567 DLFYWLLAVLSLLNFLSYLF 569

BLAST of Carg15369 vs. ExPASy Swiss-Prot
Match: Q56XQ6 (Protein NRT1/ PTR FAMILY 4.4 OS=Arabidopsis thaliana OX=3702 GN=NPF4.4 PE=2 SV=1)

HSP 1 Score: 413.3 bits (1061), Expect = 4.6e-114
Identity = 234/565 (41.42%), Postives = 341/565 (60.35%), Query Frame = 0

Query: 12  VDWKGRSAFKQKHGGTKSSLLALVTYGFDQMANFALAVNLTTYFNTVMHLELADAANQLT 71
           VD++GR     KHGGT+++L  L    F+ MA  A+  NL TY    MH  L+ +AN +T
Sbjct: 27  VDFRGRPCRPSKHGGTRAALFVLGFQAFEMMAIAAVGNNLITYVFNEMHFPLSKSANLVT 86

Query: 72  NYMGTCYILSIPMAVLADTCLGRVKTVIISGCLEFLALILLMIQAHYPKLKPPLCNIFDK 131
           N++GT ++LS+    L+D+ LG  +T+++ G +E    ILL +QAH P+L+PP CN+   
Sbjct: 87  NFIGTVFLLSLLGGFLSDSYLGSFRTMLVFGVIEISGFILLSVQAHLPELRPPECNMKST 146

Query: 132 QSHCETVGGGNAALLYVPLYALAIGNAGIKAALPPHGADQFDEKDPKEAMQISSFFNQLL 191
             HC    G  AA LY  L  +A+G+  +K  +  HGA+QF  KD +   ++SSFFN   
Sbjct: 147 TIHCVEANGYKAATLYTALCLVALGSGCLKPNIISHGANQFQRKDLR---KLSSFFNAAY 206

Query: 192 LGSSLGIAASLTLVVWIQDNKGWDWGFGVSSAAILFGIVMFVAGLPLYRMHIISGSNAIV 251
              S+G   +LTL+VW+Q + G D GFGVS+A +  G++  VAG   YR    SGS    
Sbjct: 207 FAFSMGQLIALTLLVWVQTHSGMDVGFGVSAAVMAAGMISLVAGTSFYRNKPPSGS-IFT 266

Query: 252 QVLQVYVAATRNRNLVLPEDPADLYEIERDKEAVMEEDFLPHRNICRFLDKAAIQQTPCR 311
            + QV+VAA   R  + P +P  +++   D   V     L H N  RFLDKA      C 
Sbjct: 267 PIAQVFVAAITKRKQICPSNPNMVHQPSTDLVRVKP---LLHSNKFRFLDKA------CI 326

Query: 312 QVETPEASSPWKLCTVTQVENAKVILSMIPIFCCTVNMSLCFAQLQTFSIQQGLTMDTKL 371
           + +     SPW+LCT+ QV   K++LS+IPIF CT+  +   AQLQTFS+QQG +M+T +
Sbjct: 327 KTQGKAMESPWRLCTIEQVHQVKILLSVIPIFACTIIFNTILAQLQTFSVQQGSSMNTHI 386

Query: 372 TPSFKIPPASLTIIPVFFLIIMAPIYDKILVPFARKFTGIPTGITPLQRVGVGLVLSIIS 431
           T +F+IPPASL  IP   LI   P+Y+   VP ARK TG  +GI+PLQR+G GL L+  S
Sbjct: 387 TKTFQIPPASLQAIPYIILIFFVPLYETFFVPLARKLTGNDSGISPLQRIGTGLFLATFS 446

Query: 432 MAVAALVEVKRKGVARDHNMLDATPVLQPLPISTFWLSFQFFIFGIADLFAYVGLLDFFY 491
           M  AALVE KR+    + N++          +S FW++ QF IFG++++F  VGL++FFY
Sbjct: 447 MVAAALVEKKRRESFLEQNVM----------LSIFWIAPQFLIFGLSEMFTAVGLVEFFY 506

Query: 492 SEAPKALKSVSTCFLWSSMGMGYFLSTIIVKVVNSAT--KGITKNGGWLIGNNINRNHLN 551
            ++ ++++S  T   + S   G++LS+++V  VN  T   G     GWL  N++N++ L+
Sbjct: 507 KQSSQSMQSFLTAMTYCSYSFGFYLSSVLVSTVNRVTSSNGSGTKEGWLGDNDLNKDRLD 566

Query: 552 LFYWLLSILSFINFFIYIFATQKYN 575
            FYWLL+ LSFINFF Y+F ++ Y+
Sbjct: 567 HFYWLLASLSFINFFNYLFWSRWYS 568

BLAST of Carg15369 vs. ExPASy Swiss-Prot
Match: Q9FM20 (Protein NRT1/ PTR FAMILY 4.7 OS=Arabidopsis thaliana OX=3702 GN=NPF4.7 PE=2 SV=2)

HSP 1 Score: 378.6 bits (971), Expect = 1.3e-103
Identity = 229/588 (38.95%), Postives = 337/588 (57.31%), Query Frame = 0

Query: 5   LEVVEGKVDWKGRSAFKQKHGGTKSSLLALVTYGFDQMANFALAVNLTTYFNTVMHLELA 64
           L    G VDW+ R A + +HGG  ++   LV    + +A  A A NL  Y +T M    +
Sbjct: 38  LSAWNGYVDWRSRPALRGRHGGMLAASFVLVVEVLENLAFLANASNLVLYLSTKMGFSPS 97

Query: 65  DAANQLTNYMGTCYILSIPMAVLADTCLGRVKTVIISGCLEFLALILLMIQAHYPKLKPP 124
            AAN +T +MGT + L++    LAD         ++S  +EFL L++L +QAH    +P 
Sbjct: 98  GAANAVTAFMGTAFFLALLGGFLADAFFTTFHIYLVSAAIEFLGLMVLTVQAHEHSTEP- 157

Query: 125 LCNIFDKQSHCETVGGGNAALLYVPLYALAIGNAGIKAALPPHGADQFDEKDPKEAMQIS 184
                            +   L+V LY +A+G  GIK +LPPHGA+QFDE+      Q S
Sbjct: 158 ----------------WSRVFLFVGLYLVALGVGGIKGSLPPHGAEQFDEETSSGRRQRS 217

Query: 185 SFFNQLLLGSSLGIAASLTLVVWIQDNKGWDWGFGVSSAAILFGIVMFVAGLPLYRMHII 244
            FFN  +   S G   ++T+VVW++DNKGW +GFGVS+AAIL  + +F+AG  +YR+ + 
Sbjct: 218 FFFNYFIFSLSCGALIAVTVVVWLEDNKGWSYGFGVSTAAILISVPVFLAGSRVYRLKVP 277

Query: 245 SGSNAIVQVLQVYVAATRNRNLVLPEDPADLYEIERDKEAVMEEDFLPHRNICRFLDKAA 304
           SGS  I  + +V  AA   +           Y+  R    V+       RN C   D + 
Sbjct: 278 SGS-PITTLFKVLTAALYAK-----------YKKRRTSRIVVT---CHTRNDC---DDSV 337

Query: 305 IQQ----------TPCRQVETPEASSPWKL-CTVTQVENAKVILSMIPIFCCTVNMSLCF 364
            +Q          +   +V     S P  L CT  QV++ K+++ ++PIF  T+ ++ C 
Sbjct: 338 TKQNCDGDDGFLGSFLGEVVRERESLPRPLRCTEEQVKDVKIVIKILPIFMSTIMLNCCL 397

Query: 365 AQLQTFSIQQGLTMDTKLTPSFKIPPASLTIIPVFFLIIMAPIYDKILVPFARKFTGIPT 424
           AQL TFS+QQ  TM+TKL  SF +PPA+L + PV F++I+AP Y+ +L+P ARK T   T
Sbjct: 398 AQLSTFSVQQASTMNTKL-GSFTVPPAALPVFPVVFMMILAPTYNHLLLPLARKSTKTET 457

Query: 425 GITPLQRVGVGLVLSIISMAVAALVEVKRKGVARD--HNMLDATPVLQPLPISTFWLSFQ 484
           GIT LQR+G GLVLSI++MAVAALVE KRK V      N   ++    PLPI+  W++ Q
Sbjct: 458 GITHLQRIGTGLVLSIVAMAVAALVETKRKHVVVSCCSNNNSSSYSSSPLPITFLWVAIQ 517

Query: 485 FFIFGIADLFAYVGLLDFFYSEAPKALKSVSTCFLWSSMGMGYFLSTIIVKVVNSATKGI 544
           +   G ADLF   G+++FF++EAP  ++S++T   W+S+ MGY+ S+++V  VN  T G+
Sbjct: 518 YVFLGSADLFTLAGMMEFFFTEAPSTMRSLATSLSWASLAMGYYFSSVLVSAVNFVT-GL 577

Query: 545 TKNGGWLIGNNINRNHLNLFYWLLSILSFINFFIYIFATQKYNYRNHK 580
             +  WL+G N+N+ HL  FYWL+ +LS INF  Y+F   +Y YR+++
Sbjct: 578 NHHNPWLLGENLNQYHLERFYWLMCVLSGINFLHYLFWASRYVYRSNQ 588

BLAST of Carg15369 vs. ExPASy TrEMBL
Match: A0A6J1ECP0 (protein NRT1/ PTR FAMILY 4.6-like OS=Cucurbita moschata OX=3662 GN=LOC111433115 PE=3 SV=1)

HSP 1 Score: 1154.4 bits (2985), Expect = 0.0e+00
Identity = 588/589 (99.83%), Postives = 588/589 (99.83%), Query Frame = 0

Query: 1   MEEMLEVVEGKVDWKGRSAFKQKHGGTKSSLLALVTYGFDQMANFALAVNLTTYFNTVMH 60
           MEEMLEVVEGKVDWKGRSAFKQKHGGTKSSLLALVTYGFDQMANFALAVNLTTYFNTVMH
Sbjct: 1   MEEMLEVVEGKVDWKGRSAFKQKHGGTKSSLLALVTYGFDQMANFALAVNLTTYFNTVMH 60

Query: 61  LELADAANQLTNYMGTCYILSIPMAVLADTCLGRVKTVIISGCLEFLALILLMIQAHYPK 120
           LELADAANQLTNYMGTCYILSIPMAVLADTCLGRVKTVIISGCLEFLALILLMIQAHYPK
Sbjct: 61  LELADAANQLTNYMGTCYILSIPMAVLADTCLGRVKTVIISGCLEFLALILLMIQAHYPK 120

Query: 121 LKPPLCNIFDKQSHCETVGGGNAALLYVPLYALAIGNAGIKAALPPHGADQFDEKDPKEA 180
           LKPPLCNIFDKQSHCETVGGGNAALLYVPLYALAIGNAGIKAALPPHGADQFDEKDPKEA
Sbjct: 121 LKPPLCNIFDKQSHCETVGGGNAALLYVPLYALAIGNAGIKAALPPHGADQFDEKDPKEA 180

Query: 181 MQISSFFNQLLLGSSLGIAASLTLVVWIQDNKGWDWGFGVSSAAILFGIVMFVAGLPLYR 240
           MQISSFFNQLLLGSSLGIAASLTLVVWIQDNKGWDWGFGVSSAAILFGIVMFVAGLPLYR
Sbjct: 181 MQISSFFNQLLLGSSLGIAASLTLVVWIQDNKGWDWGFGVSSAAILFGIVMFVAGLPLYR 240

Query: 241 MHIISGSNAIVQVLQVYVAATRNRNLVLPEDPADLYEIERDKEAVMEEDFLPHRNICRFL 300
           MHIISGSNAIVQVLQVYVAATRNRNLVLPEDPADLYEIERDKEAVMEEDFLPHRNICRFL
Sbjct: 241 MHIISGSNAIVQVLQVYVAATRNRNLVLPEDPADLYEIERDKEAVMEEDFLPHRNICRFL 300

Query: 301 DKAAIQQTPCRQVETPEASSPWKLCTVTQVENAKVILSMIPIFCCTVNMSLCFAQLQTFS 360
           DKAAIQQTPCRQVETPEASSPWKLCTVTQVENAKVILSMIPIFCCTVNMSLCFAQLQTFS
Sbjct: 301 DKAAIQQTPCRQVETPEASSPWKLCTVTQVENAKVILSMIPIFCCTVNMSLCFAQLQTFS 360

Query: 361 IQQGLTMDTKLTPSFKIPPASLTIIPVFFLIIMAPIYDKILVPFARKFTGIPTGITPLQR 420
           IQQGLTMDTKLTPSFKIPPASLTIIPVFFLIIMAPIYDKILVPFARKFTGIPTGITPLQR
Sbjct: 361 IQQGLTMDTKLTPSFKIPPASLTIIPVFFLIIMAPIYDKILVPFARKFTGIPTGITPLQR 420

Query: 421 VGVGLVLSIISMAVAALVEVKRKGVARDHNMLDATPVLQPLPISTFWLSFQFFIFGIADL 480
           VGVGLVLSIISMAVAALVEVKRKGVARDHNMLDATPVLQPLPISTFWLSFQFFIFGIADL
Sbjct: 421 VGVGLVLSIISMAVAALVEVKRKGVARDHNMLDATPVLQPLPISTFWLSFQFFIFGIADL 480

Query: 481 FAYVGLLDFFYSEAPKALKSVSTCFLWSSMGMGYFLSTIIVKVVNSATKGITKNGGWLIG 540
           FAYVGLLDFFYSEAPKALKSVSTCFLWSSMGMGYFLSTIIVKVVNSATKGITKNGGWLIG
Sbjct: 481 FAYVGLLDFFYSEAPKALKSVSTCFLWSSMGMGYFLSTIIVKVVNSATKGITKNGGWLIG 540

Query: 541 NNINRNHLNLFYWLLSILSFINFFIYIFATQKYNYRNHKPAISAIDDRR 590
           NNINRNHLNLFYWLLSILSFINFFIYIFATQKYNYRNHKPAISAIDD R
Sbjct: 541 NNINRNHLNLFYWLLSILSFINFFIYIFATQKYNYRNHKPAISAIDDSR 589

BLAST of Carg15369 vs. ExPASy TrEMBL
Match: A0A6J1INK1 (protein NRT1/ PTR FAMILY 4.6-like isoform X1 OS=Cucurbita maxima OX=3661 GN=LOC111478568 PE=3 SV=1)

HSP 1 Score: 1115.5 bits (2884), Expect = 0.0e+00
Identity = 570/589 (96.77%), Postives = 576/589 (97.79%), Query Frame = 0

Query: 1   MEEMLEVVEGKVDWKGRSAFKQKHGGTKSSLLALVTYGFDQMANFALAVNLTTYFNTVMH 60
           MEEMLEVVEGKVDWKGRSAFKQKHGGTKSSLL LVTYGFDQMANFALAVNLTTYFNTVMH
Sbjct: 1   MEEMLEVVEGKVDWKGRSAFKQKHGGTKSSLLTLVTYGFDQMANFALAVNLTTYFNTVMH 60

Query: 61  LELADAANQLTNYMGTCYILSIPMAVLADTCLGRVKTVIISGCLEFLALILLMIQAHYPK 120
           LELADAANQLTNYMGTCYILSIPMAVLADTCLGRVKTVIISGCLEFLALILLMIQAHYPK
Sbjct: 61  LELADAANQLTNYMGTCYILSIPMAVLADTCLGRVKTVIISGCLEFLALILLMIQAHYPK 120

Query: 121 LKPPLCNIFDKQSHCETVGGGNAALLYVPLYALAIGNAGIKAALPPHGADQFDEKDPKEA 180
           LKPPLCNIFDKQSHCETVGGGNAALLYVPLYALAIGNAGIKAALPPHGADQFDEKDPKEA
Sbjct: 121 LKPPLCNIFDKQSHCETVGGGNAALLYVPLYALAIGNAGIKAALPPHGADQFDEKDPKEA 180

Query: 181 MQISSFFNQLLLGSSLGIAASLTLVVWIQDNKGWDWGFGVSSAAILFGIVMFVAGLPLYR 240
           MQISSFFNQLLLGSSLGIAASLTLVVWIQD+KGWDWGFGVSSAAILFG+VMFVAGLP YR
Sbjct: 181 MQISSFFNQLLLGSSLGIAASLTLVVWIQDDKGWDWGFGVSSAAILFGMVMFVAGLPRYR 240

Query: 241 MHIISGSNAIVQVLQVYVAATRNRNLVLPEDPADLYEIERDKEAVMEEDFLPHRNICRFL 300
           MHIISGSNAIVQVLQVYVAATRNRNLVLPEDPADLYEIERDKEAVMEEDFLPHRNI RFL
Sbjct: 241 MHIISGSNAIVQVLQVYVAATRNRNLVLPEDPADLYEIERDKEAVMEEDFLPHRNIFRFL 300

Query: 301 DKAAIQQTPCRQVETPEASSPWKLCTVTQVENAKVILSMIPIFCCTVNMSLCFAQLQTFS 360
           DKAAIQQTPCRQVE PEASSPWKLCTVTQVENAKVILSMIPIFCCTVNMSLCFAQLQTFS
Sbjct: 301 DKAAIQQTPCRQVEIPEASSPWKLCTVTQVENAKVILSMIPIFCCTVNMSLCFAQLQTFS 360

Query: 361 IQQGLTMDTKLTPSFKIPPASLTIIPVFFLIIMAPIYDKILVPFARKFTGIPTGITPLQR 420
           IQQGLTMDTKLTPSF IPP SLTIIPVFFLI+MAPIYDKILVPFARK TGIPTGITPLQR
Sbjct: 361 IQQGLTMDTKLTPSFNIPPPSLTIIPVFFLILMAPIYDKILVPFARKLTGIPTGITPLQR 420

Query: 421 VGVGLVLSIISMAVAALVEVKRKGVARDHNMLDATPVLQPLPISTFWLSFQFFIFGIADL 480
           VGVGLVLSIISMAVAALVEVKRKGVARDHNMLDATPVLQPLPISTFWLSFQFFIFGIADL
Sbjct: 421 VGVGLVLSIISMAVAALVEVKRKGVARDHNMLDATPVLQPLPISTFWLSFQFFIFGIADL 480

Query: 481 FAYVGLLDFFYSEAPKALKSVSTCFLWSSMGMGYFLSTIIVKVVNSATKGITKNGGWLIG 540
           FAYVGLL+FFYSEAPKALKSVSTCFLWSSMGMGYFLSTIIVK VNSATKGIT+NGGWLIG
Sbjct: 481 FAYVGLLEFFYSEAPKALKSVSTCFLWSSMGMGYFLSTIIVKTVNSATKGITRNGGWLIG 540

Query: 541 NNINRNHLNLFYWLLSILSFINFFIYIFATQKYNYRNHKPAISAIDDRR 590
           NNINRNHLNLFYWLLSILSFINFFIYIFATQKY+YRNH P   AIDD R
Sbjct: 541 NNINRNHLNLFYWLLSILSFINFFIYIFATQKYSYRNHNP---AIDDSR 586

BLAST of Carg15369 vs. ExPASy TrEMBL
Match: A0A6J1ILR5 (protein NRT1/ PTR FAMILY 4.6-like isoform X2 OS=Cucurbita maxima OX=3661 GN=LOC111478568 PE=3 SV=1)

HSP 1 Score: 1035.8 bits (2677), Expect = 7.0e-299
Identity = 530/548 (96.72%), Postives = 536/548 (97.81%), Query Frame = 0

Query: 42  MANFALAVNLTTYFNTVMHLELADAANQLTNYMGTCYILSIPMAVLADTCLGRVKTVIIS 101
           MANFALAVNLTTYFNTVMHLELADAANQLTNYMGTCYILSIPMAVLADTCLGRVKTVIIS
Sbjct: 1   MANFALAVNLTTYFNTVMHLELADAANQLTNYMGTCYILSIPMAVLADTCLGRVKTVIIS 60

Query: 102 GCLEFLALILLMIQAHYPKLKPPLCNIFDKQSHCETVGGGNAALLYVPLYALAIGNAGIK 161
           GCLEFLALILLMIQAHYPKLKPPLCNIFDKQSHCETVGGGNAALLYVPLYALAIGNAGIK
Sbjct: 61  GCLEFLALILLMIQAHYPKLKPPLCNIFDKQSHCETVGGGNAALLYVPLYALAIGNAGIK 120

Query: 162 AALPPHGADQFDEKDPKEAMQISSFFNQLLLGSSLGIAASLTLVVWIQDNKGWDWGFGVS 221
           AALPPHGADQFDEKDPKEAMQISSFFNQLLLGSSLGIAASLTLVVWIQD+KGWDWGFGVS
Sbjct: 121 AALPPHGADQFDEKDPKEAMQISSFFNQLLLGSSLGIAASLTLVVWIQDDKGWDWGFGVS 180

Query: 222 SAAILFGIVMFVAGLPLYRMHIISGSNAIVQVLQVYVAATRNRNLVLPEDPADLYEIERD 281
           SAAILFG+VMFVAGLP YRMHIISGSNAIVQVLQVYVAATRNRNLVLPEDPADLYEIERD
Sbjct: 181 SAAILFGMVMFVAGLPRYRMHIISGSNAIVQVLQVYVAATRNRNLVLPEDPADLYEIERD 240

Query: 282 KEAVMEEDFLPHRNICRFLDKAAIQQTPCRQVETPEASSPWKLCTVTQVENAKVILSMIP 341
           KEAVMEEDFLPHRNI RFLDKAAIQQTPCRQVE PEASSPWKLCTVTQVENAKVILSMIP
Sbjct: 241 KEAVMEEDFLPHRNIFRFLDKAAIQQTPCRQVEIPEASSPWKLCTVTQVENAKVILSMIP 300

Query: 342 IFCCTVNMSLCFAQLQTFSIQQGLTMDTKLTPSFKIPPASLTIIPVFFLIIMAPIYDKIL 401
           IFCCTVNMSLCFAQLQTFSIQQGLTMDTKLTPSF IPP SLTIIPVFFLI+MAPIYDKIL
Sbjct: 301 IFCCTVNMSLCFAQLQTFSIQQGLTMDTKLTPSFNIPPPSLTIIPVFFLILMAPIYDKIL 360

Query: 402 VPFARKFTGIPTGITPLQRVGVGLVLSIISMAVAALVEVKRKGVARDHNMLDATPVLQPL 461
           VPFARK TGIPTGITPLQRVGVGLVLSIISMAVAALVEVKRKGVARDHNMLDATPVLQPL
Sbjct: 361 VPFARKLTGIPTGITPLQRVGVGLVLSIISMAVAALVEVKRKGVARDHNMLDATPVLQPL 420

Query: 462 PISTFWLSFQFFIFGIADLFAYVGLLDFFYSEAPKALKSVSTCFLWSSMGMGYFLSTIIV 521
           PISTFWLSFQFFIFGIADLFAYVGLL+FFYSEAPKALKSVSTCFLWSSMGMGYFLSTIIV
Sbjct: 421 PISTFWLSFQFFIFGIADLFAYVGLLEFFYSEAPKALKSVSTCFLWSSMGMGYFLSTIIV 480

Query: 522 KVVNSATKGITKNGGWLIGNNINRNHLNLFYWLLSILSFINFFIYIFATQKYNYRNHKPA 581
           K VNSATKGIT+NGGWLIGNNINRNHLNLFYWLLSILSFINFFIYIFATQKY+YRNH P 
Sbjct: 481 KTVNSATKGITRNGGWLIGNNINRNHLNLFYWLLSILSFINFFIYIFATQKYSYRNHNP- 540

Query: 582 ISAIDDRR 590
             AIDD R
Sbjct: 541 --AIDDSR 545

BLAST of Carg15369 vs. ExPASy TrEMBL
Match: A0A6J1KVN2 (protein NRT1/ PTR FAMILY 4.5-like OS=Cucurbita maxima OX=3661 GN=LOC111496854 PE=3 SV=1)

HSP 1 Score: 966.1 bits (2496), Expect = 6.8e-278
Identity = 491/579 (84.80%), Postives = 522/579 (90.16%), Query Frame = 0

Query: 1   MEEMLEVVEGKVDWKGRSAFKQKHGGTKSSLLALVTYGFDQMANFALAVNLTTYFNTVMH 60
           M+E  EVVEGKVDWKGR AFK KHGGT+SSLL LV +GF+ MA FALAVNL TYFNTVMH
Sbjct: 1   MQEKFEVVEGKVDWKGRPAFKHKHGGTRSSLLILVAFGFENMATFALAVNLITYFNTVMH 60

Query: 61  LELADAANQLTNYMGTCYILSIPMAVLADTCLGRVKTVIISGCLEFLALILLMIQAHYPK 120
            ELADAANQLTNYMG  YILSI MAVLADT LGR KTVIISGCLEFLALILLMIQAHYPK
Sbjct: 61  FELADAANQLTNYMGAGYILSILMAVLADTFLGRFKTVIISGCLEFLALILLMIQAHYPK 120

Query: 121 LKPPLCNIFDKQSHCETVGGGNAALLYVPLYALAIGNAGIKAALPPHGADQFDEKDPKEA 180
           LKP  CN+FDKQ+HCETVGGGNAA LYV LY LAIG+AGIKAALP HGADQFDEKDPKEA
Sbjct: 121 LKPQPCNMFDKQAHCETVGGGNAAFLYVALYTLAIGSAGIKAALPSHGADQFDEKDPKEA 180

Query: 181 MQISSFFNQLLLGSSLGIAASLTLVVWIQDNKGWDWGFGVSSAAILFGIVMFVAGLPLYR 240
           +Q+SSFFNQLLLG   G AASLTL+VWIQD KGWDWG G+SSAAI FG+V+F AGLPLYR
Sbjct: 181 LQMSSFFNQLLLGVCGGGAASLTLIVWIQDYKGWDWGLGISSAAIFFGVVIFTAGLPLYR 240

Query: 241 MHIISGSNAIVQVLQVYVAATRNRNLVLPEDPADLYEIERDKEAVMEEDFLPHRNICRFL 300
           MHI+S S+AIVQ+LQVYVAA RNRNLVLPED ADLYEIERDKEA +EEDFLPHRNI RFL
Sbjct: 241 MHIVSESSAIVQILQVYVAAIRNRNLVLPEDSADLYEIERDKEASVEEDFLPHRNIYRFL 300

Query: 301 DKAAIQQTPCRQVETPEASSPWKLCTVTQVENAKVILSMIPIFCCTVNMSLCFAQLQTFS 360
           DKAAIQQTPC QVETPE SSPWKLC VTQVENAKVILSM+PIFCCT+ M+LC AQLQTFS
Sbjct: 301 DKAAIQQTPCGQVETPEVSSPWKLCRVTQVENAKVILSMVPIFCCTIVMTLCLAQLQTFS 360

Query: 361 IQQGLTMDTKLTPSFKIPPASLTIIPVFFLIIMAPIYDKILVPFARKFTGIPTGITPLQR 420
           IQQGLTMDTKLT SF IPPASL IIPV F+I++ PIYDKI VPFARK TGIPTGIT LQR
Sbjct: 361 IQQGLTMDTKLTNSFHIPPASLVIIPVSFIILIVPIYDKIFVPFARKLTGIPTGITHLQR 420

Query: 421 VGVGLVLSIISMAVAALVEVKRKGVARDHNMLDATPVLQPLPISTFWLSFQFFIFGIADL 480
           VGVGLVLS ISMAVAAL+E KRKGVARDHNMLDATPVLQPLPISTFWLSFQFFIFGIADL
Sbjct: 421 VGVGLVLSSISMAVAALMEAKRKGVARDHNMLDATPVLQPLPISTFWLSFQFFIFGIADL 480

Query: 481 FAYVGLLDFFYSEAPKALKSVSTCFLWSSMGMGYFLSTIIVKVVNSATKGITKNGGWLIG 540
           F YVGLL+FFYSEAPK+LKSVSTCFLWSSM +GYFLSTI+VK+VNSATKGIT NGGWLIG
Sbjct: 481 FTYVGLLEFFYSEAPKSLKSVSTCFLWSSMALGYFLSTIVVKIVNSATKGITSNGGWLIG 540

Query: 541 NNINRNHLNLFYWLLSILSFINFFIYIFATQKYNYRNHK 580
           NNINRNHLNLFYWLLSILSFINF IYI   ++Y YRNHK
Sbjct: 541 NNINRNHLNLFYWLLSILSFINFCIYISVAKRYKYRNHK 579

BLAST of Carg15369 vs. ExPASy TrEMBL
Match: A0A6J1EKR5 (protein NRT1/ PTR FAMILY 4.5-like OS=Cucurbita moschata OX=3662 GN=LOC111433503 PE=3 SV=1)

HSP 1 Score: 964.9 bits (2493), Expect = 1.5e-277
Identity = 488/579 (84.28%), Postives = 523/579 (90.33%), Query Frame = 0

Query: 1   MEEMLEVVEGKVDWKGRSAFKQKHGGTKSSLLALVTYGFDQMANFALAVNLTTYFNTVMH 60
           M+E  EVVEGKVDWKGR AFK KHGGT+SSLL LV +GF+ MA FALAVNL TYFNTVMH
Sbjct: 1   MQEKFEVVEGKVDWKGRPAFKHKHGGTRSSLLILVAFGFENMATFALAVNLITYFNTVMH 60

Query: 61  LELADAANQLTNYMGTCYILSIPMAVLADTCLGRVKTVIISGCLEFLALILLMIQAHYPK 120
            ELADAANQLTNYMG  YILSI MAVLADT LGR KTVIISGCLEFLALILLMIQAHYPK
Sbjct: 61  FELADAANQLTNYMGAGYILSILMAVLADTFLGRFKTVIISGCLEFLALILLMIQAHYPK 120

Query: 121 LKPPLCNIFDKQSHCETVGGGNAALLYVPLYALAIGNAGIKAALPPHGADQFDEKDPKEA 180
           LKP  CN+FDKQ+HCETVGGGNAA LYV LY LAIG+AGIKAALP HGADQFDEKDPKEA
Sbjct: 121 LKPQPCNMFDKQAHCETVGGGNAAFLYVALYTLAIGSAGIKAALPSHGADQFDEKDPKEA 180

Query: 181 MQISSFFNQLLLGSSLGIAASLTLVVWIQDNKGWDWGFGVSSAAILFGIVMFVAGLPLYR 240
           +Q+SSFFNQLLLG   G AASLTL+VWIQD KGWDWG G+SSAAI FG+V+F AGLPLYR
Sbjct: 181 LQMSSFFNQLLLGVCGGGAASLTLIVWIQDYKGWDWGLGISSAAIFFGVVIFTAGLPLYR 240

Query: 241 MHIISGSNAIVQVLQVYVAATRNRNLVLPEDPADLYEIERDKEAVMEEDFLPHRNICRFL 300
           MHI+S S+AIVQ+LQVYVAA RNRNLVLPED ADLYEIERDKEA +EEDFLPHRNI RFL
Sbjct: 241 MHIVSESSAIVQILQVYVAAIRNRNLVLPEDSADLYEIERDKEASVEEDFLPHRNIYRFL 300

Query: 301 DKAAIQQTPCRQVETPEASSPWKLCTVTQVENAKVILSMIPIFCCTVNMSLCFAQLQTFS 360
           DKAAIQQTPC QVETPE SSPWK+C VTQVENAKVILSM+PIFCCT+ M+LC AQLQTFS
Sbjct: 301 DKAAIQQTPCGQVETPEVSSPWKICRVTQVENAKVILSMVPIFCCTIVMTLCLAQLQTFS 360

Query: 361 IQQGLTMDTKLTPSFKIPPASLTIIPVFFLIIMAPIYDKILVPFARKFTGIPTGITPLQR 420
           IQQGLTMDTKLT SF IPPASL IIPV F+I++ PIYDK+ VPFARK TGIPTGIT LQR
Sbjct: 361 IQQGLTMDTKLTNSFHIPPASLVIIPVSFIILIVPIYDKLFVPFARKLTGIPTGITHLQR 420

Query: 421 VGVGLVLSIISMAVAALVEVKRKGVARDHNMLDATPVLQPLPISTFWLSFQFFIFGIADL 480
           VGVGLVLS ISMAVAAL+E KRKGVARDHNMLDATPVLQPLPISTFWLSFQFFIFGIADL
Sbjct: 421 VGVGLVLSSISMAVAALMEAKRKGVARDHNMLDATPVLQPLPISTFWLSFQFFIFGIADL 480

Query: 481 FAYVGLLDFFYSEAPKALKSVSTCFLWSSMGMGYFLSTIIVKVVNSATKGITKNGGWLIG 540
           F YVGLL+FFYSEAPK+LKSVSTCFLWSSM +GYFLSTI+VK+VNSATKGIT+NGGWLIG
Sbjct: 481 FTYVGLLEFFYSEAPKSLKSVSTCFLWSSMALGYFLSTIVVKIVNSATKGITRNGGWLIG 540

Query: 541 NNINRNHLNLFYWLLSILSFINFFIYIFATQKYNYRNHK 580
           NNINRNHLNLFYWLLSILSFINF IY+   ++Y YRNHK
Sbjct: 541 NNINRNHLNLFYWLLSILSFINFCIYVSVAKRYKYRNHK 579

BLAST of Carg15369 vs. TAIR 10
Match: AT1G69850.1 (nitrate transporter 1:2 )

HSP 1 Score: 448.7 bits (1153), Expect = 7.0e-126
Identity = 256/598 (42.81%), Postives = 370/598 (61.87%), Query Frame = 0

Query: 1   MEEMLEVVEGKVDWKGRSAFKQKHGGTKSSLLALVTYGFDQMANFALAVNLTTYFNTVMH 60
           +EE +   EG  DW+ R+A K +HGG  ++   LV    + +A  A A NL  Y    MH
Sbjct: 3   VEEEVSRWEGYADWRNRAAVKGRHGGMLAASFVLVVEILENLAYLANASNLVLYLREYMH 62

Query: 61  LELADAANQLTNYMGTCYILSIPMAVLADTCLGRVKTVIISGCLEFLALILLMIQAHYPK 120
           +  + +AN +TN+MGT ++L++    L+D      +  +IS  +EFL LI+L IQA  P 
Sbjct: 63  MSPSKSANDVTNFMGTAFLLALLGGFLSDAFFSTFQIFLISASIEFLGLIILTIQARTPS 122

Query: 121 LKPPLCNIFDKQSHCETVGGGNAALLYVPLYALAIGNAGIKAALPPHGADQFDEKDPKEA 180
           L PP C+       CE V G  AA+L+V LY +A+G  GIK +L  HGA+QFDE  PK  
Sbjct: 123 LMPPSCD----SPTCEEVSGSKAAMLFVGLYLVALGVGGIKGSLASHGAEQFDESTPKGR 182

Query: 181 MQISSFFNQLLLGSSLGIAASLTLVVWIQDNKGWDWGFGVSSAAILFGIVMFVAGLPLYR 240
            Q S+FFN  +   + G   ++T VVW++DNKGW+WGFGVS+ AI   I++F++G   YR
Sbjct: 183 KQRSTFFNYFVFCLACGALVAVTFVVWLEDNKGWEWGFGVSTIAIFVSILIFLSGSRFYR 242

Query: 241 MHIISGSNAIVQVLQVYVAA---------TRNRNLVLPEDPAD--------LYEIERDKE 300
             I  GS  +  +L+V +AA         + N    +   P++          E + + E
Sbjct: 243 NKIPCGS-PLTTILKVLLAASVKCCSSGSSSNAVASMSVSPSNHCVSKGKKEVESQGELE 302

Query: 301 AVMEEDFLPHR----NICRFLDKAAIQQTPCRQVETPEASSPWKLCTVTQVENAKVILSM 360
              +E+ LP R    N  + L+ AA ++   R +E          CTV QVE+ K++L M
Sbjct: 303 KPRQEEALPPRAQLTNSLKVLNGAADEKPVHRLLE----------CTVQQVEDVKIVLKM 362

Query: 361 IPIFCCTVNMSLCFAQLQTFSIQQGLTMDTKLTPSFKIPPASLTIIPVFFLIIMAPIYDK 420
           +PIF CT+ ++ C AQL TFS+QQ  +M+TK+  S KIPPASL I PV F++I+APIYD 
Sbjct: 363 LPIFACTIMLNCCLAQLSTFSVQQAASMNTKI-GSLKIPPASLPIFPVVFIMILAPIYDH 422

Query: 421 ILVPFARKFTGIPTGITPLQRVGVGLVLSIISMAVAALVEVKRKGVARDHNMLDATPVLQ 480
           +++PFARK T   TG+T LQR+GVGLVLSI++MAVAALVE+KRKGVA+D  +LD+    +
Sbjct: 423 LIIPFARKATKTETGVTHLQRIGVGLVLSILAMAVAALVEIKRKGVAKDSGLLDSK---E 482

Query: 481 PLPISTFWLSFQFFIFGIADLFAYVGLLDFFYSEAPKALKSVSTCFLWSSMGMGYFLSTI 540
            LP++  W++ Q+   G ADLF   GLL++F++EAP +++S++T   W+S+ MGY+LS++
Sbjct: 483 TLPVTFLWIALQYLFLGSADLFTLAGLLEYFFTEAPSSMRSLATSLSWASLAMGYYLSSV 542

Query: 541 IVKVVNSATKGITKNGGWLIGNNINRNHLNLFYWLLSILSFINFFIYIFATQKYNYRN 578
           IV +VNS T G + N  WL G +INR  L+ FYWL+ +LS  NF  Y+F   +Y YR+
Sbjct: 543 IVSIVNSIT-GSSGNTPWLRGKSINRYKLDYFYWLMCVLSAANFLHYLFWAMRYKYRS 580

BLAST of Carg15369 vs. TAIR 10
Match: AT1G27040.2 (Major facilitator superfamily protein )

HSP 1 Score: 420.6 bits (1080), Expect = 2.0e-117
Identity = 236/568 (41.55%), Postives = 348/568 (61.27%), Query Frame = 0

Query: 9   EGKVDWKGRSAFKQKHGGTKSSLLALVTYGFDQMANFALAVNLTTYFNTVMHLELADAAN 68
           EG  DW+ ++A + +HGG  ++   L     + +A  A A NL  Y    MH+ LA +++
Sbjct: 9   EGYADWRNKAALRGRHGGMLAASFVLAVEILENLAFLANASNLVLYLKNFMHMSLARSSS 68

Query: 69  QLTNYMGTCYILSIPMAVLADTCLGRVKTVIISGCLEFLALILLMIQAHYPKLKPPLCNI 128
           ++T +M T ++L++    LAD         +IS  +EFL LILL IQA  P L PP C  
Sbjct: 69  EVTTFMATAFLLALLGGFLADAFFSTFVIFLISASIEFLGLILLTIQARRPSLMPPPCK- 128

Query: 129 FDKQSHCETVGGGNAALLYVPLYALAIGNAGIKAALPPHGADQFDEKDPKEAMQISSFFN 188
                 CE VGG  AA L+V LY +++G  GIK +LP HGA+QFDE  PK   Q S+FFN
Sbjct: 129 SSAALRCEVVGGSKAAFLFVGLYLVSLGIGGIKGSLPSHGAEQFDEGTPKGRKQRSTFFN 188

Query: 189 QLLLGSSLGIAASLTLVVWIQDNKGWDWGFGVSSAAILFGIVMFVAGLPLYRMHIISGSN 248
             +   S G   ++T VVWI+DNKGW+WGFGVS+ +I   I++F+ G   Y+  I  GS 
Sbjct: 189 YYVFCLSCGALVAVTFVVWIEDNKGWEWGFGVSTISIFLSILVFLLGSRFYKNKIPRGS- 248

Query: 249 AIVQVLQVYVAAT-RNRNLVLPEDPADLYEIERDKEAVMEEDFLPHRNICRFLDKAAIQQ 308
            +  + +V +AA+  + +     +     E++ + E       L +   C  L+KA   +
Sbjct: 249 PLTTIFKVLLAASIVSCSSKTSSNHFTSREVQSEHEEKTPSQSLTNSLTC--LNKAIEGK 308

Query: 309 TPCRQVETPEASSPWKLCTVTQVENAKVILSMIPIFCCTVNMSLCFAQLQTFSIQQGLTM 368
           T             W  CTV QVE+ K++L M+PIF CT+ ++ C AQL T+S+ Q  TM
Sbjct: 309 T----------HHIWLECTVQQVEDVKIVLKMLPIFGCTIMLNCCLAQLSTYSVHQAATM 368

Query: 369 DTKLTPSFKIPPASLTIIPVFFLIIMAPIYDKILVPFARKFTGIPTGITPLQRVGVGLVL 428
           + K+  +F +P ASL + PV F++I+AP YD +++PFARK T    GIT LQR+GVGLVL
Sbjct: 369 NRKIV-NFNVPSASLPVFPVVFMLILAPTYDHLIIPFARKVTKSEIGITHLQRIGVGLVL 428

Query: 429 SIISMAVAALVEVKRKGVARDHNMLDATPVLQPLPISTFWLSFQFFIFGIADLFAYVGLL 488
           SI++MAVAALVE+KRK VAR+  +LD+    + LPI+  W++ Q+   G ADLF   GLL
Sbjct: 429 SIVAMAVAALVELKRKQVAREAGLLDSE---ETLPITFLWIALQYLFLGSADLFTLAGLL 488

Query: 489 DFFYSEAPKALKSVSTCFLWSSMGMGYFLSTIIVKVVNSATKGITKNGGWLIGNNINRNH 548
           +FF++EAP +++S++T   W+S+ +GY+LS+++V +VN  TK   ++  WL G  +NRN 
Sbjct: 489 EFFFTEAPSSMRSLATSLSWASLALGYYLSSVMVPIVNRVTKSAGQS-PWL-GEKLNRNR 548

Query: 549 LNLFYWLLSILSFINFFIYIFATQKYNY 576
           L+LFYWL+ +LS +NF  Y+F  ++Y Y
Sbjct: 549 LDLFYWLMCVLSVVNFLHYLFWAKRYKY 556

BLAST of Carg15369 vs. TAIR 10
Match: AT1G27040.1 (Major facilitator superfamily protein )

HSP 1 Score: 420.6 bits (1080), Expect = 2.0e-117
Identity = 236/568 (41.55%), Postives = 348/568 (61.27%), Query Frame = 0

Query: 9   EGKVDWKGRSAFKQKHGGTKSSLLALVTYGFDQMANFALAVNLTTYFNTVMHLELADAAN 68
           EG  DW+ ++A + +HGG  ++   L     + +A  A A NL  Y    MH+ LA +++
Sbjct: 13  EGYADWRNKAALRGRHGGMLAASFVLAVEILENLAFLANASNLVLYLKNFMHMSLARSSS 72

Query: 69  QLTNYMGTCYILSIPMAVLADTCLGRVKTVIISGCLEFLALILLMIQAHYPKLKPPLCNI 128
           ++T +M T ++L++    LAD         +IS  +EFL LILL IQA  P L PP C  
Sbjct: 73  EVTTFMATAFLLALLGGFLADAFFSTFVIFLISASIEFLGLILLTIQARRPSLMPPPCK- 132

Query: 129 FDKQSHCETVGGGNAALLYVPLYALAIGNAGIKAALPPHGADQFDEKDPKEAMQISSFFN 188
                 CE VGG  AA L+V LY +++G  GIK +LP HGA+QFDE  PK   Q S+FFN
Sbjct: 133 SSAALRCEVVGGSKAAFLFVGLYLVSLGIGGIKGSLPSHGAEQFDEGTPKGRKQRSTFFN 192

Query: 189 QLLLGSSLGIAASLTLVVWIQDNKGWDWGFGVSSAAILFGIVMFVAGLPLYRMHIISGSN 248
             +   S G   ++T VVWI+DNKGW+WGFGVS+ +I   I++F+ G   Y+  I  GS 
Sbjct: 193 YYVFCLSCGALVAVTFVVWIEDNKGWEWGFGVSTISIFLSILVFLLGSRFYKNKIPRGS- 252

Query: 249 AIVQVLQVYVAAT-RNRNLVLPEDPADLYEIERDKEAVMEEDFLPHRNICRFLDKAAIQQ 308
            +  + +V +AA+  + +     +     E++ + E       L +   C  L+KA   +
Sbjct: 253 PLTTIFKVLLAASIVSCSSKTSSNHFTSREVQSEHEEKTPSQSLTNSLTC--LNKAIEGK 312

Query: 309 TPCRQVETPEASSPWKLCTVTQVENAKVILSMIPIFCCTVNMSLCFAQLQTFSIQQGLTM 368
           T             W  CTV QVE+ K++L M+PIF CT+ ++ C AQL T+S+ Q  TM
Sbjct: 313 T----------HHIWLECTVQQVEDVKIVLKMLPIFGCTIMLNCCLAQLSTYSVHQAATM 372

Query: 369 DTKLTPSFKIPPASLTIIPVFFLIIMAPIYDKILVPFARKFTGIPTGITPLQRVGVGLVL 428
           + K+  +F +P ASL + PV F++I+AP YD +++PFARK T    GIT LQR+GVGLVL
Sbjct: 373 NRKIV-NFNVPSASLPVFPVVFMLILAPTYDHLIIPFARKVTKSEIGITHLQRIGVGLVL 432

Query: 429 SIISMAVAALVEVKRKGVARDHNMLDATPVLQPLPISTFWLSFQFFIFGIADLFAYVGLL 488
           SI++MAVAALVE+KRK VAR+  +LD+    + LPI+  W++ Q+   G ADLF   GLL
Sbjct: 433 SIVAMAVAALVELKRKQVAREAGLLDSE---ETLPITFLWIALQYLFLGSADLFTLAGLL 492

Query: 489 DFFYSEAPKALKSVSTCFLWSSMGMGYFLSTIIVKVVNSATKGITKNGGWLIGNNINRNH 548
           +FF++EAP +++S++T   W+S+ +GY+LS+++V +VN  TK   ++  WL G  +NRN 
Sbjct: 493 EFFFTEAPSSMRSLATSLSWASLALGYYLSSVMVPIVNRVTKSAGQS-PWL-GEKLNRNR 552

Query: 549 LNLFYWLLSILSFINFFIYIFATQKYNY 576
           L+LFYWL+ +LS +NF  Y+F  ++Y Y
Sbjct: 553 LDLFYWLMCVLSVVNFLHYLFWAKRYKY 560

BLAST of Carg15369 vs. TAIR 10
Match: AT1G59740.1 (Major facilitator superfamily protein )

HSP 1 Score: 419.5 bits (1077), Expect = 4.6e-117
Identity = 237/560 (42.32%), Postives = 337/560 (60.18%), Query Frame = 0

Query: 9   EGKVDWKGRSAFKQKHGGTKSSLLALVTYGFDQMANFALAVNLTTYFNTVMHLELADAAN 68
           E  VDW+GR +   KHGG +++L  L    F+ M   A+  NL TY    MH  L+ AAN
Sbjct: 27  EESVDWRGRPSNPNKHGGMRAALFVLGLQAFEIMGIAAVGNNLITYVINEMHFPLSKAAN 86

Query: 69  QLTNYMGTCYILSIPMAVLADTCLGRVKTVIISGCLEFLALILLMIQAHYPKLKPPLCNI 128
            +TN++GT +I ++    L+D  LG   T+II G +E    ILL +QAH P+LKPP CN 
Sbjct: 87  IVTNFVGTIFIFALLGGYLSDAFLGSFWTIIIFGFVELSGFILLSVQAHLPQLKPPKCNP 146

Query: 129 FDKQSHCETVGGGNAALLYVPLYALAIGNAGIKAALPPHGADQFDEKDPKEAMQISSFFN 188
              Q+ CE   G  A + ++ LY +A+G+  +K  +  HGADQF +  PK++ ++SS+FN
Sbjct: 147 LIDQT-CEEAKGFKAMIFFMALYLVALGSGCVKPNMIAHGADQFSQSHPKQSKRLSSYFN 206

Query: 189 QLLLGSSLGIAASLTLVVWIQDNKGWDWGFGVSSAAILFGIVMFVAGLPLYRMHIISGSN 248
                 S+G   +LTL+VW+Q + G D GFGVS+AA+  GI+  V+G  +Y  +     +
Sbjct: 207 AAYFAFSMGELIALTLLVWVQTHSGMDIGFGVSAAAMTMGIISLVSG-TMYFRNKRPRRS 266

Query: 249 AIVQVLQVYVAATRNRNLVLPEDPADLYEIERDKEAVMEEDFLPHRNICRFLDKAAIQQT 308
               +  V VAA   R L  P DP  L+        V+    LPH    RFLDKA I   
Sbjct: 267 IFTPIAHVIVAAILKRKLASPSDPRMLHGDHHVANDVVPSSTLPHTPRFRFLDKACI--- 326

Query: 309 PCRQVETPEASSPWKLCTVTQVENAKVILSMIPIFCCTVNMSLCFAQLQTFSIQQGLTMD 368
             +  +T    SPW+LCTVTQVE  K ++S++PIF  T+  +   AQLQTFS+QQG +M+
Sbjct: 327 --KIQDTNTKESPWRLCTVTQVEQVKTLISLVPIFASTIVFNTILAQLQTFSVQQGSSMN 386

Query: 369 TKLTPSFKIPPASLTIIPVFFLIIMAPIYDKILVPFARKFTGIPTGITPLQRVGVGLVLS 428
           T+L+ SF IPPASL  IP   LI + P+YD  LVPFARK TG  +GI PL R+G+GL LS
Sbjct: 387 TRLSNSFHIPPASLQAIPYIMLIFLVPLYDSFLVPFARKLTGHNSGIPPLTRIGIGLFLS 446

Query: 429 IISMAVAALVEVKRKGVARDHNMLDATPVLQPLPISTFWLSFQFFIFGIADLFAYVGLLD 488
             SM  AA++E KR    RD ++LD         +S FW++ QF IFGI+++F  VGL++
Sbjct: 447 TFSMVSAAMLEKKR----RDSSVLDGR------ILSIFWITPQFLIFGISEMFTAVGLIE 506

Query: 489 FFYSEAPKALKSVSTCFLWSSMGMGYFLSTIIVKVVNSATKGITKNGGWLIGNNINRNHL 548
           FFY ++ K ++S      + S   G++ S+++V VVN  T     + GWL  N++N++ L
Sbjct: 507 FFYKQSAKGMESFLMALTYCSYSFGFYFSSVLVSVVNKITSTSVDSKGWLGENDLNKDRL 566

Query: 549 NLFYWLLSILSFINFFIYIF 569
           +LFYWLL++LS +NF  Y+F
Sbjct: 567 DLFYWLLAVLSLLNFLSYLF 569

BLAST of Carg15369 vs. TAIR 10
Match: AT1G33440.1 (Major facilitator superfamily protein )

HSP 1 Score: 413.3 bits (1061), Expect = 3.3e-115
Identity = 234/565 (41.42%), Postives = 341/565 (60.35%), Query Frame = 0

Query: 12  VDWKGRSAFKQKHGGTKSSLLALVTYGFDQMANFALAVNLTTYFNTVMHLELADAANQLT 71
           VD++GR     KHGGT+++L  L    F+ MA  A+  NL TY    MH  L+ +AN +T
Sbjct: 27  VDFRGRPCRPSKHGGTRAALFVLGFQAFEMMAIAAVGNNLITYVFNEMHFPLSKSANLVT 86

Query: 72  NYMGTCYILSIPMAVLADTCLGRVKTVIISGCLEFLALILLMIQAHYPKLKPPLCNIFDK 131
           N++GT ++LS+    L+D+ LG  +T+++ G +E    ILL +QAH P+L+PP CN+   
Sbjct: 87  NFIGTVFLLSLLGGFLSDSYLGSFRTMLVFGVIEISGFILLSVQAHLPELRPPECNMKST 146

Query: 132 QSHCETVGGGNAALLYVPLYALAIGNAGIKAALPPHGADQFDEKDPKEAMQISSFFNQLL 191
             HC    G  AA LY  L  +A+G+  +K  +  HGA+QF  KD +   ++SSFFN   
Sbjct: 147 TIHCVEANGYKAATLYTALCLVALGSGCLKPNIISHGANQFQRKDLR---KLSSFFNAAY 206

Query: 192 LGSSLGIAASLTLVVWIQDNKGWDWGFGVSSAAILFGIVMFVAGLPLYRMHIISGSNAIV 251
              S+G   +LTL+VW+Q + G D GFGVS+A +  G++  VAG   YR    SGS    
Sbjct: 207 FAFSMGQLIALTLLVWVQTHSGMDVGFGVSAAVMAAGMISLVAGTSFYRNKPPSGS-IFT 266

Query: 252 QVLQVYVAATRNRNLVLPEDPADLYEIERDKEAVMEEDFLPHRNICRFLDKAAIQQTPCR 311
            + QV+VAA   R  + P +P  +++   D   V     L H N  RFLDKA      C 
Sbjct: 267 PIAQVFVAAITKRKQICPSNPNMVHQPSTDLVRVKP---LLHSNKFRFLDKA------CI 326

Query: 312 QVETPEASSPWKLCTVTQVENAKVILSMIPIFCCTVNMSLCFAQLQTFSIQQGLTMDTKL 371
           + +     SPW+LCT+ QV   K++LS+IPIF CT+  +   AQLQTFS+QQG +M+T +
Sbjct: 327 KTQGKAMESPWRLCTIEQVHQVKILLSVIPIFACTIIFNTILAQLQTFSVQQGSSMNTHI 386

Query: 372 TPSFKIPPASLTIIPVFFLIIMAPIYDKILVPFARKFTGIPTGITPLQRVGVGLVLSIIS 431
           T +F+IPPASL  IP   LI   P+Y+   VP ARK TG  +GI+PLQR+G GL L+  S
Sbjct: 387 TKTFQIPPASLQAIPYIILIFFVPLYETFFVPLARKLTGNDSGISPLQRIGTGLFLATFS 446

Query: 432 MAVAALVEVKRKGVARDHNMLDATPVLQPLPISTFWLSFQFFIFGIADLFAYVGLLDFFY 491
           M  AALVE KR+    + N++          +S FW++ QF IFG++++F  VGL++FFY
Sbjct: 447 MVAAALVEKKRRESFLEQNVM----------LSIFWIAPQFLIFGLSEMFTAVGLVEFFY 506

Query: 492 SEAPKALKSVSTCFLWSSMGMGYFLSTIIVKVVNSAT--KGITKNGGWLIGNNINRNHLN 551
            ++ ++++S  T   + S   G++LS+++V  VN  T   G     GWL  N++N++ L+
Sbjct: 507 KQSSQSMQSFLTAMTYCSYSFGFYLSSVLVSTVNRVTSSNGSGTKEGWLGDNDLNKDRLD 566

Query: 552 LFYWLLSILSFINFFIYIFATQKYN 575
            FYWLL+ LSFINFF Y+F ++ Y+
Sbjct: 567 HFYWLLASLSFINFFNYLFWSRWYS 568

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
KAG6581385.10.0e+00100.00Protein NRT1/ PTR FAMILY 4.6, partial [Cucurbita argyrosperma subsp. sororia] >K... [more]
XP_022925822.10.0e+0099.83protein NRT1/ PTR FAMILY 4.6-like [Cucurbita moschata][more]
XP_023543943.10.0e+0098.64protein NRT1/ PTR FAMILY 4.6-like [Cucurbita pepo subsp. pepo][more]
XP_022978676.10.0e+0096.77protein NRT1/ PTR FAMILY 4.6-like isoform X1 [Cucurbita maxima][more]
XP_023522562.11.4e-30698.54protein NRT1/ PTR FAMILY 4.6-like [Cucurbita pepo subsp. pepo][more]
Match NameE-valueIdentityDescription
Q8H1579.9e-12542.81Protein NRT1/ PTR FAMILY 4.6 OS=Arabidopsis thaliana OX=3702 GN=NPF4.6 PE=1 SV=1[more]
Q8VYE42.9e-11641.55Protein NRT1/ PTR FAMILY 4.5 OS=Arabidopsis thaliana OX=3702 GN=NPF4.5 PE=2 SV=1[more]
Q93VV56.4e-11642.32Protein NRT1/ PTR FAMILY 4.3 OS=Arabidopsis thaliana OX=3702 GN=NPF4.3 PE=2 SV=1[more]
Q56XQ64.6e-11441.42Protein NRT1/ PTR FAMILY 4.4 OS=Arabidopsis thaliana OX=3702 GN=NPF4.4 PE=2 SV=1[more]
Q9FM201.3e-10338.95Protein NRT1/ PTR FAMILY 4.7 OS=Arabidopsis thaliana OX=3702 GN=NPF4.7 PE=2 SV=2[more]
Match NameE-valueIdentityDescription
A0A6J1ECP00.0e+0099.83protein NRT1/ PTR FAMILY 4.6-like OS=Cucurbita moschata OX=3662 GN=LOC111433115 ... [more]
A0A6J1INK10.0e+0096.77protein NRT1/ PTR FAMILY 4.6-like isoform X1 OS=Cucurbita maxima OX=3661 GN=LOC1... [more]
A0A6J1ILR57.0e-29996.72protein NRT1/ PTR FAMILY 4.6-like isoform X2 OS=Cucurbita maxima OX=3661 GN=LOC1... [more]
A0A6J1KVN26.8e-27884.80protein NRT1/ PTR FAMILY 4.5-like OS=Cucurbita maxima OX=3661 GN=LOC111496854 PE... [more]
A0A6J1EKR51.5e-27784.28protein NRT1/ PTR FAMILY 4.5-like OS=Cucurbita moschata OX=3662 GN=LOC111433503 ... [more]
Match NameE-valueIdentityDescription
AT1G69850.17.0e-12642.81nitrate transporter 1:2 [more]
AT1G27040.22.0e-11741.55Major facilitator superfamily protein [more]
AT1G27040.12.0e-11741.55Major facilitator superfamily protein [more]
AT1G59740.14.6e-11742.32Major facilitator superfamily protein [more]
AT1G33440.13.3e-11541.42Major facilitator superfamily protein [more]
InterPro
Analysis Name: InterPro Annotations of Silver-seed gourd (SMH-JMG-627) v2
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR036259MFS transporter superfamilyGENE3D1.20.1250.20MFS general substrate transporter like domainscoord: 2..581
e-value: 1.2E-160
score: 537.2
IPR036259MFS transporter superfamilySUPERFAMILY103473MFS general substrate transportercoord: 31..570
IPR000109Proton-dependent oligopeptide transporter familyPFAMPF00854PTR2coord: 96..533
e-value: 4.1E-83
score: 279.4
IPR000109Proton-dependent oligopeptide transporter familyPANTHERPTHR11654OLIGOPEPTIDE TRANSPORTER-RELATEDcoord: 11..580
NoneNo IPR availablePANTHERPTHR11654:SF336PROTEIN NRT1/ PTR FAMILY 4.2coord: 11..580

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Carg15369-RACarg15369-RAmRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0042981 regulation of apoptotic process
biological_process GO:0055085 transmembrane transport
cellular_component GO:0016021 integral component of membrane
cellular_component GO:0016020 membrane
molecular_function GO:0022857 transmembrane transporter activity