Carg15335 (gene) Silver-seed gourd (SMH-JMG-627) v2

BLAST of Carg15335 vs. NCBI nr
Match: KAG7034635.1 (GDSL esterase/lipase, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 744.2 bits (1920), Expect = 5.3e-211
Identity = 361/361 (100.00%), Postives = 361/361 (100.00%), Query Frame = 0

Query: 1   MGSSNSSPVQSLNRLFIVLVLSLLTFAPPPVLGCFTSIFNFGDSLSDTGNLFGNCVSEEP 60
           MGSSNSSPVQSLNRLFIVLVLSLLTFAPPPVLGCFTSIFNFGDSLSDTGNLFGNCVSEEP
Sbjct: 1   MGSSNSSPVQSLNRLFIVLVLSLLTFAPPPVLGCFTSIFNFGDSLSDTGNLFGNCVSEEP 60

Query: 61  PHSCFPPYGDAFFHHPTGRFSNGRLIVDFIAQSLGLPLLPPYLSVGRRRISIQDFERGLN 120
           PHSCFPPYGDAFFHHPTGRFSNGRLIVDFIAQSLGLPLLPPYLSVGRRRISIQDFERGLN
Sbjct: 61  PHSCFPPYGDAFFHHPTGRFSNGRLIVDFIAQSLGLPLLPPYLSVGRRRISIQDFERGLN 120

Query: 121 FAVAGATALDEAVLREKGITNLPTNYSLGVQMEWFKNTYSSVCKSSFTATAKCRAILERS 180
           FAVAGATALDEAVLREKGITNLPTNYSLGVQMEWFKNTYSSVCKSSFTATAKCRAILERS
Sbjct: 121 FAVAGATALDEAVLREKGITNLPTNYSLGVQMEWFKNTYSSVCKSSFTATAKCRAILERS 180

Query: 181 LIVVGEIGGNDYNYAFIDERQNIDEIKSLVAPVVKAIGSAITELIKLGAQTLMVPGNLPI 240
           LIVVGEIGGNDYNYAFIDERQNIDEIKSLVAPVVKAIGSAITELIKLGAQTLMVPGNLPI
Sbjct: 181 LIVVGEIGGNDYNYAFIDERQNIDEIKSLVAPVVKAIGSAITELIKLGAQTLMVPGNLPI 240

Query: 241 GCNPTYLKVYGTSVQNSENGCVNWLNEFSEYHNEQLLQELKQTRALYPHIRIIYADYYNS 300
           GCNPTYLKVYGTSVQNSENGCVNWLNEFSEYHNEQLLQELKQTRALYPHIRIIYADYYNS
Sbjct: 241 GCNPTYLKVYGTSVQNSENGCVNWLNEFSEYHNEQLLQELKQTRALYPHIRIIYADYYNS 300

Query: 301 AMLFYDAPQDFGREVSIPLPEIPLPGMFCGISQSTHMGAGRHWHTTQCLAFIAFTTAQPH 360
           AMLFYDAPQDFGREVSIPLPEIPLPGMFCGISQSTHMGAGRHWHTTQCLAFIAFTTAQPH
Sbjct: 301 AMLFYDAPQDFGREVSIPLPEIPLPGMFCGISQSTHMGAGRHWHTTQCLAFIAFTTAQPH 360

Query: 361 H 362
           H
Sbjct: 361 H 361

BLAST of Carg15335 vs. NCBI nr
Match: XP_022925815.1 (GDSL esterase/lipase At1g28590-like [Cucurbita moschata])

HSP 1 Score: 627.5 bits (1617), Expect = 7.2e-176
Identity = 307/312 (98.40%), Postives = 309/312 (99.04%), Query Frame = 0

Query: 1   MGSSNSSPVQSLNRLFIVLVLSLLTFAPPPVLGCFTSIFNFGDSLSDTGNLFGNCVSEEP 60
           MGSSNSSPVQSLNRLFIVLVLSLLTFAPPPVLGCFTSIFNFGDSLSDTGNLFGNCVSEEP
Sbjct: 1   MGSSNSSPVQSLNRLFIVLVLSLLTFAPPPVLGCFTSIFNFGDSLSDTGNLFGNCVSEEP 60

Query: 61  PHSCFPPYGDAFFHHPTGRFSNGRLIVDFIAQSLGLPLLPPYLSVGRRRISIQDFERGLN 120
           PHSCFPPYGDAFFHHPTGRFSNGRLIVDFIAQSLGLPLLPPYLSVGRRRISIQDFE+GLN
Sbjct: 61  PHSCFPPYGDAFFHHPTGRFSNGRLIVDFIAQSLGLPLLPPYLSVGRRRISIQDFEKGLN 120

Query: 121 FAVAGATALDEAVLREKGITNLPTNYSLGVQMEWFKNTYSSVCKSSFTATAKCRAILERS 180
           FAVAGATALDE+VLREKGITNLPTNYSLGVQMEWFKNTYSSVCKSS TATAKCRAILERS
Sbjct: 121 FAVAGATALDESVLREKGITNLPTNYSLGVQMEWFKNTYSSVCKSSSTATAKCRAILERS 180

Query: 181 LIVVGEIGGNDYNYAFIDERQNIDEIKSLVAPVVKAIGSAITELIKLGAQTLMVPGNLPI 240
           LIVVGEIGGNDYNYAFIDERQNIDEIKSLV PVVKAIGSAITELIKLGAQTLMVPGNLPI
Sbjct: 181 LIVVGEIGGNDYNYAFIDERQNIDEIKSLVTPVVKAIGSAITELIKLGAQTLMVPGNLPI 240

Query: 241 GCNPTYLKVYGTSVQNSENGCVNWLNEFSEYHNEQLLQELKQTRALYPHIRIIYADYYNS 300
           GCNPTYLKVYGTSVQNSENGCVNWLNEFSEYHNEQLLQELKQ RALYPHIRIIYADYYNS
Sbjct: 241 GCNPTYLKVYGTSVQNSENGCVNWLNEFSEYHNEQLLQELKQIRALYPHIRIIYADYYNS 300

Query: 301 AMLFYDAPQDFG 313
           AMLFYDAPQDFG
Sbjct: 301 AMLFYDAPQDFG 312

BLAST of Carg15335 vs. NCBI nr
Match: XP_022978715.1 (GDSL esterase/lipase At1g28570-like [Cucurbita maxima])

HSP 1 Score: 602.8 bits (1553), Expect = 1.9e-168
Identity = 292/312 (93.59%), Postives = 303/312 (97.12%), Query Frame = 0

Query: 1   MGSSNSSPVQSLNRLFIVLVLSLLTFAPPPVLGCFTSIFNFGDSLSDTGNLFGNCVSEEP 60
           MGSSNSSPV SLNRLFI+LVL LLTFAPPPVLGCFTSIFNFGDSLSDTGNLFG CVSEEP
Sbjct: 1   MGSSNSSPVLSLNRLFIILVLPLLTFAPPPVLGCFTSIFNFGDSLSDTGNLFGKCVSEEP 60

Query: 61  PHSCFPPYGDAFFHHPTGRFSNGRLIVDFIAQSLGLPLLPPYLSVGRRRISIQDFERGLN 120
           PHSCFPPYGDAFFHHPTGRFSNGRLI+DFIAQSLGLPLLPPYLSVGRRRI+I+DFE+GLN
Sbjct: 61  PHSCFPPYGDAFFHHPTGRFSNGRLIIDFIAQSLGLPLLPPYLSVGRRRINIEDFEKGLN 120

Query: 121 FAVAGATALDEAVLREKGITNLPTNYSLGVQMEWFKNTYSSVCKSSFTATAKCRAILERS 180
           FAVAGATALDEAVLREKGITNL TNYSLGVQ+EWFKNTYSSVCKSS TATAKCRAIL+ S
Sbjct: 121 FAVAGATALDEAVLREKGITNLSTNYSLGVQLEWFKNTYSSVCKSSSTATAKCRAILKSS 180

Query: 181 LIVVGEIGGNDYNYAFIDERQNIDEIKSLVAPVVKAIGSAITELIKLGAQTLMVPGNLPI 240
           LIVVGEIGGNDYNYAFIDERQNI+EIKSLV PVVKAIGSAITELIKLGAQTLMVPGNLPI
Sbjct: 181 LIVVGEIGGNDYNYAFIDERQNIEEIKSLVPPVVKAIGSAITELIKLGAQTLMVPGNLPI 240

Query: 241 GCNPTYLKVYGTSVQNSENGCVNWLNEFSEYHNEQLLQELKQTRALYPHIRIIYADYYNS 300
           GCNPTYLKVYGTSVQN ENGCVNWLNEFS+YHNEQLL+ELKQ RALYPHIRIIYADYYNS
Sbjct: 241 GCNPTYLKVYGTSVQNPENGCVNWLNEFSQYHNEQLLEELKQIRALYPHIRIIYADYYNS 300

Query: 301 AMLFYDAPQDFG 313
           AMLFYDAP+DFG
Sbjct: 301 AMLFYDAPRDFG 312

BLAST of Carg15335 vs. NCBI nr
Match: XP_023544566.1 (GDSL esterase/lipase At1g28590-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 599.7 bits (1545), Expect = 1.6e-167
Identity = 293/312 (93.91%), Postives = 299/312 (95.83%), Query Frame = 0

Query: 1   MGSSNSSPVQSLNRLFIVLVLSLLTFAPPPVLGCFTSIFNFGDSLSDTGNLFGNCVSEEP 60
           MGSSNSSPV SLNRLFI+LVL LLTFAPP V GCFTSIFNFGDSLSDTGNLFGNCVSEEP
Sbjct: 1   MGSSNSSPVLSLNRLFIILVLPLLTFAPPTVFGCFTSIFNFGDSLSDTGNLFGNCVSEEP 60

Query: 61  PHSCFPPYGDAFFHHPTGRFSNGRLIVDFIAQSLGLPLLPPYLSVGRRRISIQDFERGLN 120
           PHSCFPPYGDAFFHHPTGRFSNGRLI+DFIAQSLGLPLL PYL VGRRRISIQDFE+GLN
Sbjct: 61  PHSCFPPYGDAFFHHPTGRFSNGRLIIDFIAQSLGLPLLQPYLGVGRRRISIQDFEKGLN 120

Query: 121 FAVAGATALDEAVLREKGITNLPTNYSLGVQMEWFKNTYSSVCKSSFTATAKCRAILERS 180
           FAVAGATALDEAVLREKGITNLPTNYSLGVQ+EWFKNTYS VCKSS TAT KCRAIL+ S
Sbjct: 121 FAVAGATALDEAVLREKGITNLPTNYSLGVQLEWFKNTYSFVCKSSSTATEKCRAILKSS 180

Query: 181 LIVVGEIGGNDYNYAFIDERQNIDEIKSLVAPVVKAIGSAITELIKLGAQTLMVPGNLPI 240
           LIVVGEIGGNDYNY FIDERQNI+EIKSLVAPVVKAIGSAITELIKLGAQTLMVPGNLPI
Sbjct: 181 LIVVGEIGGNDYNYPFIDERQNIEEIKSLVAPVVKAIGSAITELIKLGAQTLMVPGNLPI 240

Query: 241 GCNPTYLKVYGTSVQNSENGCVNWLNEFSEYHNEQLLQELKQTRALYPHIRIIYADYYNS 300
           GCNPTYLKVYGTSVQNSENGCVNWLNEFSEYHNEQLLQELKQ RALYP IRIIYADYYNS
Sbjct: 241 GCNPTYLKVYGTSVQNSENGCVNWLNEFSEYHNEQLLQELKQIRALYPRIRIIYADYYNS 300

Query: 301 AMLFYDAPQDFG 313
           AMLFYDAPQDFG
Sbjct: 301 AMLFYDAPQDFG 312

BLAST of Carg15335 vs. NCBI nr
Match: KAG6581350.1 (GDSL esterase/lipase, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 448.7 bits (1153), Expect = 4.6e-122
Identity = 221/222 (99.55%), Postives = 222/222 (100.00%), Query Frame = 0

Query: 91  AQSLGLPLLPPYLSVGRRRISIQDFERGLNFAVAGATALDEAVLREKGITNLPTNYSLGV 150
           AQSLGLPLLPPYLSVGRRRISIQDFE+GLNFAVAGATALDEAVLREKGITNLPTNYSLGV
Sbjct: 12  AQSLGLPLLPPYLSVGRRRISIQDFEKGLNFAVAGATALDEAVLREKGITNLPTNYSLGV 71

Query: 151 QMEWFKNTYSSVCKSSFTATAKCRAILERSLIVVGEIGGNDYNYAFIDERQNIDEIKSLV 210
           QMEWFKNTYSSVCKSSFTATAKCRAILERSLIVVGEIGGNDYNYAFIDERQNIDEIKSLV
Sbjct: 72  QMEWFKNTYSSVCKSSFTATAKCRAILERSLIVVGEIGGNDYNYAFIDERQNIDEIKSLV 131

Query: 211 APVVKAIGSAITELIKLGAQTLMVPGNLPIGCNPTYLKVYGTSVQNSENGCVNWLNEFSE 270
           APVVKAIGSAITELIKLGAQTLMVPGNLPIGCNPTYLKVYGTSVQNSENGCVNWLNEFSE
Sbjct: 132 APVVKAIGSAITELIKLGAQTLMVPGNLPIGCNPTYLKVYGTSVQNSENGCVNWLNEFSE 191

Query: 271 YHNEQLLQELKQTRALYPHIRIIYADYYNSAMLFYDAPQDFG 313
           YHNEQLLQELKQTRALYPHIRIIYADYYNSAMLFYDAPQDFG
Sbjct: 192 YHNEQLLQELKQTRALYPHIRIIYADYYNSAMLFYDAPQDFG 233

BLAST of Carg15335 vs. ExPASy Swiss-Prot
Match: Q9ZQI3 (GDSL esterase/lipase At2g27360 OS=Arabidopsis thaliana OX=3702 GN=At2g27360 PE=2 SV=1)

HSP 1 Score: 292.7 bits (748), Expect = 5.5e-78
Identity = 150/300 (50.00%), Postives = 201/300 (67.00%), Query Frame = 0

Query: 35  FTSIFNFGDSLSDTGNLFGNCVSEEPPHSCFPPYGDAFFHHPTGRFSNGRLIVDFIAQSL 94
           F SI +FGDS++DTGNL G     + P S FPPYG+ FFHHP+GRFS+GRLI+DFIA+ L
Sbjct: 31  FKSIISFGDSITDTGNLLGLSSPNDLPESAFPPYGETFFHHPSGRFSDGRLIIDFIAEFL 90

Query: 95  GLPLLPPYLSVGRRRISIQDFERGLNFAVAGATALDEAVLREKGITNLPTNYSLGVQMEW 154
           G+P +PP+   G +     +FE+G+NFAV GATAL+ +VL EKG     +N SLG Q++ 
Sbjct: 91  GIPHVPPF--YGSKN---GNFEKGVNFAVGGATALECSVLEEKGTHCSQSNISLGNQLKS 150

Query: 155 FKNTYSSVCKSSFTATAKCRAILERSLIVVGEIGGNDYNYAFIDERQNIDEIKSLVAPVV 214
           FK +   +C SS   +  CR ++E + I++GEIGGNDYN+   D R+NI+E+K LV  V+
Sbjct: 151 FKESLPYLCGSS---SPDCRDMIENAFILIGEIGGNDYNFPLFD-RKNIEEVKELVPLVI 210

Query: 215 KAIGSAITELIKLGAQTLMVPGNLPIGCNPTYLKVYGTSVQNSEN---GCVNWLNEFSEY 274
             I SAI+EL+ +GA+T +VPGN P+GC+  YL +Y T  +   N   GC+ WLN+FS Y
Sbjct: 211 TTISSAISELVDMGARTFLVPGNFPLGCSVAYLTLYETPNKEEYNPLTGCLTWLNDFSVY 270

Query: 275 HNEQLLQELKQTRALYPHIRIIYADYYNSAMLFYDAPQDFGREVSIPLPEIPLPGMFCGI 332
           HNEQL  ELK+ R LYPH+ IIY DYYN+ +     P  FG      L + PLP   CG+
Sbjct: 271 HNEQLQAELKRLRNLYPHVNIIYGDYYNTLLRLMQEPSKFG------LMDRPLPAC-CGL 314

BLAST of Carg15335 vs. ExPASy Swiss-Prot
Match: Q9FXJ2 (GDSL esterase/lipase At1g28580 OS=Arabidopsis thaliana OX=3702 GN=At1g28580 PE=2 SV=1)

HSP 1 Score: 288.9 bits (738), Expect = 8.0e-77
Identity = 145/281 (51.60%), Postives = 189/281 (67.26%), Query Frame = 0

Query: 35  FTSIFNFGDSLSDTGNLFGNCVSEEPPHSCFPPYGDAFFHHPTGRFSNGRLIVDFIAQSL 94
           F SI +FGDS++DTGNL G    ++ PH  FPPYG+ FFHHPTGRFSNGRLI+DFIA+ L
Sbjct: 35  FKSIISFGDSIADTGNLLGLSDPKDLPHMAFPPYGENFFHHPTGRFSNGRLIIDFIAEFL 94

Query: 95  GLPLLPPYLSVGRRRISIQDFERGLNFAVAGATALDEAVLREKGITNLPTNYSLGVQMEW 154
           GLPL+PP+           +FE+G+NFAV GATAL+ + L ++GI    TN SLGVQ+  
Sbjct: 95  GLPLVPPFYGSHN-----ANFEKGVNFAVGGATALERSFLEDRGIHFPYTNVSLGVQLNS 154

Query: 155 FKNTYSSVCKSSFTATAKCRAILERSLIVVGEIGGNDYNYAFIDERQNIDEIKSLVAPVV 214
           FK +  S+C S     + CR ++E +LI++GEIGGNDYNYAF  ++  I+EIK L+  V+
Sbjct: 155 FKESLPSICGS----PSDCRDMIENALILMGEIGGNDYNYAFFVDK-GIEEIKELMPLVI 214

Query: 215 KAIGSAITELIKLGAQTLMVPGNLPIGCNPTYLKVYGTSVQNSEN---GCVNWLNEFSEY 274
             I SAITELI +G +T +VPG  P+GC+  YL  + TS     +   GC+ WLN+F E 
Sbjct: 215 TTISSAITELIGMGGRTFLVPGEFPVGCSVLYLTSHQTSNMEEYDPLTGCLKWLNKFGEN 274

Query: 275 HNEQLLQELKQTRALYPHIRIIYADYYNSAMLFYDAPQDFG 313
           H EQL  EL + + LYPH+ IIYADYYN+    Y  P  FG
Sbjct: 275 HGEQLRAELNRLQKLYPHVNIIYADYYNALFHLYQEPAKFG 305

BLAST of Carg15335 vs. ExPASy Swiss-Prot
Match: Q8RXT9 (GDSL esterase/lipase At1g28590 OS=Arabidopsis thaliana OX=3702 GN=At1g28590 PE=2 SV=2)

HSP 1 Score: 287.7 bits (735), Expect = 1.8e-76
Identity = 155/344 (45.06%), Postives = 215/344 (62.50%), Query Frame = 0

Query: 1   MGSSNSSPVQSLNRLFIVLVLSLLTFAPPPVLGCFTSIFNFGDSLSDTGNLFGNCVSEEP 60
           M S +S P   L R FI+  L + +         F SI +FGDS++DTGNL G     + 
Sbjct: 1   MASLDSLPAMKLVR-FILSTLLVTSVNSQTQCRNFKSIISFGDSIADTGNLLGLSDPNDL 60

Query: 61  PHSCFPPYGDAFFHHPTGRFSNGRLIVDFIAQSLGLPLLPPYLSVGRRRISIQDFERGLN 120
           P S FPPYG+ FFHHPTGR+S+GRLI+DFIA+ LG PL+PP+           +F++G+N
Sbjct: 61  PASAFPPYGETFFHHPTGRYSDGRLIIDFIAEFLGFPLVPPFYGCQN-----ANFKKGVN 120

Query: 121 FAVAGATALDEAVLREKGITNLPTNYSLGVQMEWFKNTYSSVCKSSFTATAKCRAILERS 180
           FAVAGATAL+ + L E+GI +  TN SL VQ+  F  +  ++C S     + CR ++E +
Sbjct: 121 FAVAGATALEPSFLEERGIHSTITNVSLSVQLRSFTESLPNLCGS----PSDCRDMIENA 180

Query: 181 LIVVGEIGGNDYNYAFIDERQNIDEIKSLVAPVVKAIGSAITELIKLGAQTLMVPGNLPI 240
           LI++GEIGGNDYN+A   +R+ + E++ LV  V+  I SAITEL+ +G +T +VPGN PI
Sbjct: 181 LILMGEIGGNDYNFALF-QRKPVKEVEELVPFVIATISSAITELVCMGGRTFLVPGNFPI 240

Query: 241 GCNPTYLKVYGTSVQNSEN---GCVNWLNEFSEYHNEQLLQELKQTRALYPHIRIIYADY 300
           G + +YL +Y TS +   +   GC+ WLN+FSEY+N+QL +EL   R LYPH+ IIYADY
Sbjct: 241 GYSASYLTLYKTSNKEEYDPLTGCLKWLNDFSEYYNKQLQEELNGLRKLYPHVNIIYADY 300

Query: 301 YNSAMLFYDAPQDFGREVSIPLPEIPLPGMFCGISQSTHMGAGR 342
           YN+ +  +  P  FG          PLP   CG+  S +    R
Sbjct: 301 YNALLRLFQEPAKFG------FMNRPLPAC-CGVGGSYNFNFSR 326

BLAST of Carg15335 vs. ExPASy Swiss-Prot
Match: Q9FXJ1 (GDSL esterase/lipase At1g28570 OS=Arabidopsis thaliana OX=3702 GN=At1g28570 PE=3 SV=1)

HSP 1 Score: 286.2 bits (731), Expect = 5.2e-76
Identity = 153/307 (49.84%), Postives = 197/307 (64.17%), Query Frame = 0

Query: 17  IVLVLSLLTFAPPPVLGCFTSIFNFGDSLSDTGNLFGNCVSEEPPHSCFPPYGDAFFHHP 76
           I+    L T    P    F SI +FGDS++DTGNL         P   F PYG+ FFHHP
Sbjct: 14  ILSTFCLTTVNSEPQCHNFKSIISFGDSIADTGNLLALSDPTNLPKVAFLPYGETFFHHP 73

Query: 77  TGRFSNGRLIVDFIAQSLGLPLLPPYLSVGRRRISIQDFERGLNFAVAGATALDEAVLRE 136
           TGRFSNGRLI+DFIA+ LG PL+PP+           +FE+G+NFAV GATAL+ + L E
Sbjct: 74  TGRFSNGRLIIDFIAEFLGFPLVPPFYGSQN-----ANFEKGVNFAVGGATALERSFLEE 133

Query: 137 KGITNLPTNYSLGVQMEWFKNTYSSVCKSSFTATAKCRAILERSLIVVGEIGGNDYNYAF 196
           +GI    TN SL VQ+  FK +  ++C S     + CR ++E SLI++GEIGGNDYNYAF
Sbjct: 134 RGIHFPYTNVSLAVQLSSFKESLPNLCVS----PSDCRDMIENSLILMGEIGGNDYNYAF 193

Query: 197 IDERQNIDEIKSLVAPVVKAIGSAITELIKLGAQTLMVPGNLPIGCNPTYLKVYGTS--- 256
               +NI+EIK LV  V++ I SAITELI +G +T +VPG  P+GC+  YL +Y TS   
Sbjct: 194 F-VGKNIEEIKELVPLVIETISSAITELIGMGGKTFLVPGEFPLGCSVAYLSLYQTSNIE 253

Query: 257 VQNSENGCVNWLNEFSEYHNEQLLQELKQTRALYPHIRIIYADYYNSAMLFYDAPQDFGR 316
             +   GC+ WLN+FSEYH+EQL  EL + + LYPH+ IIYADYYN+ +     P  FG 
Sbjct: 254 EYDPLTGCLKWLNKFSEYHDEQLQAELNRLQKLYPHVNIIYADYYNTLLRLAQEPAKFG- 309

Query: 317 EVSIPLP 321
            +S PLP
Sbjct: 314 FISRPLP 309

BLAST of Carg15335 vs. ExPASy Swiss-Prot
Match: Q3E7I6 (GDSL esterase/lipase At1g28650 OS=Arabidopsis thaliana OX=3702 GN=At1g28650 PE=2 SV=1)

HSP 1 Score: 278.1 bits (710), Expect = 1.4e-73
Identity = 143/280 (51.07%), Postives = 190/280 (67.86%), Query Frame = 0

Query: 35  FTSIFNFGDSLSDTGNLFGNCVSEEPPHSCFPPYGDAFFHHPTGRFSNGRLIVDFIAQSL 94
           + SI +FGDS++DTGN          P + F PYG++FFH P+GR+S+GRL++DFIA+ L
Sbjct: 35  YKSIISFGDSIADTGNYVHLSNVNNLPQAAFLPYGESFFHPPSGRYSDGRLVIDFIAEFL 94

Query: 95  GLPLLPPYLSVGRRRISIQDFERGLNFAVAGATALDEAVLREKGITNLPTNYSLGVQMEW 154
           GLP +PPY   G + +S   F +G+NFAV GATALD A L ++GI +  TN SL VQ+  
Sbjct: 95  GLPYVPPYF--GSQNVS---FNQGINFAVYGATALDRAFLVKQGIKSDFTNISLSVQLNT 154

Query: 155 FKNTYSSVCKSSFTATAKCRAILERSLIVVGEIGGNDYNYAFIDERQNIDEIKSLVAPVV 214
           FK    ++C SS   T  CR +L  SLI++GEIGGNDYNY F  E ++I+EIK LV  ++
Sbjct: 155 FKQILPNLCASS---TRDCREMLGDSLILMGEIGGNDYNYPFF-EGKSINEIKELVPLII 214

Query: 215 KAIGSAITELIKLGAQTLMVPGNLPIGCNPTYLKVYGTSVQNSE--NGCVNWLNEFSEYH 274
           KAI SAI +LI LG +T +VPGN PIGC+  YL ++ T+    +   GC+ WLN+F E+H
Sbjct: 215 KAISSAIVDLIDLGGKTFLVPGNFPIGCSTAYLTLFQTATVEHDPFTGCIPWLNKFGEHH 274

Query: 275 NEQLLQELKQTRALYPHIRIIYADYYNSAMLFYDAPQDFG 313
           NEQL  ELKQ + LYPH+ IIYADYYNS    +  P  +G
Sbjct: 275 NEQLKIELKQLQKLYPHVNIIYADYYNSLYGLFQEPAKYG 305

BLAST of Carg15335 vs. ExPASy TrEMBL
Match: A0A6J1EJ98 (GDSL esterase/lipase At1g28590-like OS=Cucurbita moschata OX=3662 GN=LOC111433109 PE=3 SV=1)

HSP 1 Score: 627.5 bits (1617), Expect = 3.5e-176
Identity = 307/312 (98.40%), Postives = 309/312 (99.04%), Query Frame = 0

Query: 1   MGSSNSSPVQSLNRLFIVLVLSLLTFAPPPVLGCFTSIFNFGDSLSDTGNLFGNCVSEEP 60
           MGSSNSSPVQSLNRLFIVLVLSLLTFAPPPVLGCFTSIFNFGDSLSDTGNLFGNCVSEEP
Sbjct: 1   MGSSNSSPVQSLNRLFIVLVLSLLTFAPPPVLGCFTSIFNFGDSLSDTGNLFGNCVSEEP 60

Query: 61  PHSCFPPYGDAFFHHPTGRFSNGRLIVDFIAQSLGLPLLPPYLSVGRRRISIQDFERGLN 120
           PHSCFPPYGDAFFHHPTGRFSNGRLIVDFIAQSLGLPLLPPYLSVGRRRISIQDFE+GLN
Sbjct: 61  PHSCFPPYGDAFFHHPTGRFSNGRLIVDFIAQSLGLPLLPPYLSVGRRRISIQDFEKGLN 120

Query: 121 FAVAGATALDEAVLREKGITNLPTNYSLGVQMEWFKNTYSSVCKSSFTATAKCRAILERS 180
           FAVAGATALDE+VLREKGITNLPTNYSLGVQMEWFKNTYSSVCKSS TATAKCRAILERS
Sbjct: 121 FAVAGATALDESVLREKGITNLPTNYSLGVQMEWFKNTYSSVCKSSSTATAKCRAILERS 180

Query: 181 LIVVGEIGGNDYNYAFIDERQNIDEIKSLVAPVVKAIGSAITELIKLGAQTLMVPGNLPI 240
           LIVVGEIGGNDYNYAFIDERQNIDEIKSLV PVVKAIGSAITELIKLGAQTLMVPGNLPI
Sbjct: 181 LIVVGEIGGNDYNYAFIDERQNIDEIKSLVTPVVKAIGSAITELIKLGAQTLMVPGNLPI 240

Query: 241 GCNPTYLKVYGTSVQNSENGCVNWLNEFSEYHNEQLLQELKQTRALYPHIRIIYADYYNS 300
           GCNPTYLKVYGTSVQNSENGCVNWLNEFSEYHNEQLLQELKQ RALYPHIRIIYADYYNS
Sbjct: 241 GCNPTYLKVYGTSVQNSENGCVNWLNEFSEYHNEQLLQELKQIRALYPHIRIIYADYYNS 300

Query: 301 AMLFYDAPQDFG 313
           AMLFYDAPQDFG
Sbjct: 301 AMLFYDAPQDFG 312

BLAST of Carg15335 vs. ExPASy TrEMBL
Match: A0A6J1IR01 (GDSL esterase/lipase At1g28570-like OS=Cucurbita maxima OX=3661 GN=LOC111478589 PE=3 SV=1)

HSP 1 Score: 602.8 bits (1553), Expect = 9.3e-169
Identity = 292/312 (93.59%), Postives = 303/312 (97.12%), Query Frame = 0

Query: 1   MGSSNSSPVQSLNRLFIVLVLSLLTFAPPPVLGCFTSIFNFGDSLSDTGNLFGNCVSEEP 60
           MGSSNSSPV SLNRLFI+LVL LLTFAPPPVLGCFTSIFNFGDSLSDTGNLFG CVSEEP
Sbjct: 1   MGSSNSSPVLSLNRLFIILVLPLLTFAPPPVLGCFTSIFNFGDSLSDTGNLFGKCVSEEP 60

Query: 61  PHSCFPPYGDAFFHHPTGRFSNGRLIVDFIAQSLGLPLLPPYLSVGRRRISIQDFERGLN 120
           PHSCFPPYGDAFFHHPTGRFSNGRLI+DFIAQSLGLPLLPPYLSVGRRRI+I+DFE+GLN
Sbjct: 61  PHSCFPPYGDAFFHHPTGRFSNGRLIIDFIAQSLGLPLLPPYLSVGRRRINIEDFEKGLN 120

Query: 121 FAVAGATALDEAVLREKGITNLPTNYSLGVQMEWFKNTYSSVCKSSFTATAKCRAILERS 180
           FAVAGATALDEAVLREKGITNL TNYSLGVQ+EWFKNTYSSVCKSS TATAKCRAIL+ S
Sbjct: 121 FAVAGATALDEAVLREKGITNLSTNYSLGVQLEWFKNTYSSVCKSSSTATAKCRAILKSS 180

Query: 181 LIVVGEIGGNDYNYAFIDERQNIDEIKSLVAPVVKAIGSAITELIKLGAQTLMVPGNLPI 240
           LIVVGEIGGNDYNYAFIDERQNI+EIKSLV PVVKAIGSAITELIKLGAQTLMVPGNLPI
Sbjct: 181 LIVVGEIGGNDYNYAFIDERQNIEEIKSLVPPVVKAIGSAITELIKLGAQTLMVPGNLPI 240

Query: 241 GCNPTYLKVYGTSVQNSENGCVNWLNEFSEYHNEQLLQELKQTRALYPHIRIIYADYYNS 300
           GCNPTYLKVYGTSVQN ENGCVNWLNEFS+YHNEQLL+ELKQ RALYPHIRIIYADYYNS
Sbjct: 241 GCNPTYLKVYGTSVQNPENGCVNWLNEFSQYHNEQLLEELKQIRALYPHIRIIYADYYNS 300

Query: 301 AMLFYDAPQDFG 313
           AMLFYDAP+DFG
Sbjct: 301 AMLFYDAPRDFG 312

BLAST of Carg15335 vs. ExPASy TrEMBL
Match: A0A6J1CMY9 (GDSL esterase/lipase At1g28600 OS=Momordica charantia OX=3673 GN=LOC111012455 PE=3 SV=1)

HSP 1 Score: 411.4 bits (1056), Expect = 4.0e-111
Identity = 210/313 (67.09%), Postives = 249/313 (79.55%), Query Frame = 0

Query: 1   MGSSNSSPVQSLNRLFIVLVLSLLTFAPPPVLGC-FTSIFNFGDSLSDTGNLFGNCVSEE 60
           M SSNSS + SL  +F + V S   FA P   GC ++SIFNFGDSLSDTGNLF  C S E
Sbjct: 1   MDSSNSSSIISL-IIFSIAVSSAFAFA-PRASGCGYSSIFNFGDSLSDTGNLFDECSSGE 60

Query: 61  PPHSCFPPYGDAFFHHPTGRFSNGRLIVDFIAQSLGLPLLPPYLSVGRRRISIQDFERGL 120
           P  SCF PYG+ FF  PTGR S+GRLI+DFIA+SLGLPL+ PYL V R+ I+ +DFE+GL
Sbjct: 61  PHKSCFAPYGETFFRRPTGRCSDGRLILDFIAESLGLPLVQPYLGVRRKGITAEDFEKGL 120

Query: 121 NFAVAGATALDEAVLREKGITNLPTNYSLGVQMEWFKNTYSSVCKSSFTATAKCRAILER 180
           NFAVAGATALD + LRE     + TNYSL VQ+EWF++ YSSVC+SS  +TA+CR IL+ 
Sbjct: 121 NFAVAGATALDASFLRENMSCKVHTNYSLAVQLEWFRDAYSSVCRSS--STARCREILKS 180

Query: 181 SLIVVGEIGGNDYNYAFIDERQNIDEIKSLVAPVVKAIGSAITELIKLGAQTLMVPGNLP 240
           SL++VGEIGGNDYN  F  ER++ ++IKSLV  VVK I S I ELIKLGA+TLMVPGNLP
Sbjct: 181 SLVLVGEIGGNDYNDPFF-ERRSFEDIKSLVPLVVKQIASTIIELIKLGAETLMVPGNLP 240

Query: 241 IGCNPTYLKVYGTSVQNSENGCVNWLNEFSEYHNEQLLQELKQTRALYPHIRIIYADYYN 300
           IGCNPTYLK+YGTSV+NSENGC+ WLNEFSEYHNE L +ELKQ RA YPH+ +IYADY+N
Sbjct: 241 IGCNPTYLKLYGTSVKNSENGCLEWLNEFSEYHNELLKEELKQIRANYPHVHLIYADYHN 300

Query: 301 SAMLFYDAPQDFG 313
           SAM F++APQDFG
Sbjct: 301 SAMRFFNAPQDFG 308

BLAST of Carg15335 vs. ExPASy TrEMBL
Match: A0A1S3AZH8 (GDSL esterase/lipase At1g28570-like OS=Cucumis melo OX=3656 GN=LOC103484464 PE=3 SV=1)

HSP 1 Score: 406.0 bits (1042), Expect = 1.7e-109
Identity = 200/313 (63.90%), Postives = 246/313 (78.59%), Query Frame = 0

Query: 1   MGSSNSSPVQSLNRLFIVLVLSLLTFAPPPVLG-CFTSIFNFGDSLSDTGNLFGNCVSEE 60
           M SSN     SLNR  I+  ++        VLG CF SIFNFGDSLSDTGNLF NC S +
Sbjct: 1   MDSSN----LSLNRWLIIFFVATALSPSTNVLGNCFNSIFNFGDSLSDTGNLFNNCNSNK 60

Query: 61  PPHSCFPPYGDAFFHHPTGRFSNGRLIVDFIAQSLGLPLLPPYLSVGRRRISIQDFERGL 120
           PP SCF PYGD FF HPTGRFS+GRLI+DFIAQSLGLPLL PYL V  +R+S+ +FE+GL
Sbjct: 61  PPKSCFTPYGDTFFRHPTGRFSDGRLIIDFIAQSLGLPLLQPYLGVETQRMSVAEFEKGL 120

Query: 121 NFAVAGATALDEAVLREKGITNLPTNYSLGVQMEWFKNTYSSVCKSSFTATAKCRAILER 180
           NFAVAGATALD + LREK    +PTNYSL VQ+EWFK  YS  C SS  ++ +C  IL++
Sbjct: 121 NFAVAGATALDASYLREKAFVEVPTNYSLSVQLEWFKKAYSLACPSS--SSTRCTKILKK 180

Query: 181 SLIVVGEIGGNDYNYAFIDERQNIDEIKSLVAPVVKAIGSAITELIKLGAQTLMVPGNLP 240
           SL VVGEIGGNDYNY F  ++ + +EIKSLV  VVK+IGSAITELI+LGAQ+L+VPGNLP
Sbjct: 181 SLFVVGEIGGNDYNYPFF-KQHSFEEIKSLVPVVVKSIGSAITELIQLGAQSLIVPGNLP 240

Query: 241 IGCNPTYLKVYGTSVQNSENGCVNWLNEFSEYHNEQLLQELKQTRALYPHIRIIYADYYN 300
           IGC P YLK+Y TS+Q+S+NGC++WLN+FSEYHN+ L +EL + R+ +P+++IIYADY+N
Sbjct: 241 IGCCPKYLKIYSTSIQDSKNGCLDWLNQFSEYHNKHLQEELNRIRSQHPNVQIIYADYHN 300

Query: 301 SAMLFYDAPQDFG 313
           SAM FY+ P++FG
Sbjct: 301 SAMQFYNHPENFG 306

BLAST of Carg15335 vs. ExPASy TrEMBL
Match: A0A6J1EFN7 (GDSL esterase/lipase At1g28570-like OS=Cucurbita moschata OX=3662 GN=LOC111433889 PE=3 SV=1)

HSP 1 Score: 348.6 bits (893), Expect = 3.1e-92
Identity = 171/293 (58.36%), Postives = 225/293 (76.79%), Query Frame = 0

Query: 26  FAPPPVLGCFTSIFNFGDSLSDTGNLFGNCVSEEPPHSCFPPYGDAFFHHPTGRFSNGRL 85
           FA   V G +TSIFNFGDSLSDTGNL+  C S  P H CF PYG+ FFH PTGRFS+GR+
Sbjct: 26  FAVHSVFGRYTSIFNFGDSLSDTGNLYYTCSSPNPVHVCFLPYGETFFHRPTGRFSDGRV 85

Query: 86  IVDFIAQSLGLPLLPPYLSV--GRRRISIQDFERGLNFAVAGATALDEAVLREKGITNLP 145
           I+DFIA SLGLPL+ PY SV  G   IS ++F +GLNFAV GATAL+ +   E+G+ N+P
Sbjct: 86  ILDFIATSLGLPLVRPYRSVGLGEEGISAEEFRKGLNFAVGGATALNLSYFEERGVHNVP 145

Query: 146 TN-YSLGVQMEWFKNTYSSVCKSSFTATAKCRAILERSLIVVGEIGGNDYNYAFIDERQN 205
           ++  SL +Q++WFK TYSSVC SS  A++ C+ + ++SL ++GEIGGNDYN  F+ +++N
Sbjct: 146 SHAVSLEIQLDWFKKTYSSVCASS--ASSGCKDMFKKSLFIMGEIGGNDYN-DFLFDKRN 205

Query: 206 IDEIKSLVAPVVKAIGSAITELIKLGAQTLMVPGNLPIGCNPTYLKVYGTS---VQNSEN 265
           I+E+ SLV  V+K IGSAI ELI+LG  T+MVPGN PIGC P YLK++ TS     +S+N
Sbjct: 206 IEEVASLVPLVIKQIGSAIRELIELGVDTIMVPGNFPIGCVPMYLKLFKTSNGEHYDSKN 265

Query: 266 GCVNWLNEFSEYHNEQLLQELKQTRALYPHIRIIYADYYNSAMLFYDAPQDFG 313
           GC+ WLN+FSEYHNEQL QEL++  AL+PH+ +IYADY+N+AM  ++AP++FG
Sbjct: 266 GCLKWLNQFSEYHNEQLQQELERIGALHPHVHLIYADYFNAAMRLFNAPKNFG 315

BLAST of Carg15335 vs. TAIR 10
Match: AT2G27360.1 (GDSL-like Lipase/Acylhydrolase superfamily protein )

HSP 1 Score: 292.7 bits (748), Expect = 3.9e-79
Identity = 150/300 (50.00%), Postives = 201/300 (67.00%), Query Frame = 0

Query: 35  FTSIFNFGDSLSDTGNLFGNCVSEEPPHSCFPPYGDAFFHHPTGRFSNGRLIVDFIAQSL 94
           F SI +FGDS++DTGNL G     + P S FPPYG+ FFHHP+GRFS+GRLI+DFIA+ L
Sbjct: 31  FKSIISFGDSITDTGNLLGLSSPNDLPESAFPPYGETFFHHPSGRFSDGRLIIDFIAEFL 90

Query: 95  GLPLLPPYLSVGRRRISIQDFERGLNFAVAGATALDEAVLREKGITNLPTNYSLGVQMEW 154
           G+P +PP+   G +     +FE+G+NFAV GATAL+ +VL EKG     +N SLG Q++ 
Sbjct: 91  GIPHVPPF--YGSKN---GNFEKGVNFAVGGATALECSVLEEKGTHCSQSNISLGNQLKS 150

Query: 155 FKNTYSSVCKSSFTATAKCRAILERSLIVVGEIGGNDYNYAFIDERQNIDEIKSLVAPVV 214
           FK +   +C SS   +  CR ++E + I++GEIGGNDYN+   D R+NI+E+K LV  V+
Sbjct: 151 FKESLPYLCGSS---SPDCRDMIENAFILIGEIGGNDYNFPLFD-RKNIEEVKELVPLVI 210

Query: 215 KAIGSAITELIKLGAQTLMVPGNLPIGCNPTYLKVYGTSVQNSEN---GCVNWLNEFSEY 274
             I SAI+EL+ +GA+T +VPGN P+GC+  YL +Y T  +   N   GC+ WLN+FS Y
Sbjct: 211 TTISSAISELVDMGARTFLVPGNFPLGCSVAYLTLYETPNKEEYNPLTGCLTWLNDFSVY 270

Query: 275 HNEQLLQELKQTRALYPHIRIIYADYYNSAMLFYDAPQDFGREVSIPLPEIPLPGMFCGI 332
           HNEQL  ELK+ R LYPH+ IIY DYYN+ +     P  FG      L + PLP   CG+
Sbjct: 271 HNEQLQAELKRLRNLYPHVNIIYGDYYNTLLRLMQEPSKFG------LMDRPLPAC-CGL 314

BLAST of Carg15335 vs. TAIR 10
Match: AT1G28580.1 (GDSL-like Lipase/Acylhydrolase superfamily protein )

HSP 1 Score: 288.9 bits (738), Expect = 5.7e-78
Identity = 145/281 (51.60%), Postives = 189/281 (67.26%), Query Frame = 0

Query: 35  FTSIFNFGDSLSDTGNLFGNCVSEEPPHSCFPPYGDAFFHHPTGRFSNGRLIVDFIAQSL 94
           F SI +FGDS++DTGNL G    ++ PH  FPPYG+ FFHHPTGRFSNGRLI+DFIA+ L
Sbjct: 35  FKSIISFGDSIADTGNLLGLSDPKDLPHMAFPPYGENFFHHPTGRFSNGRLIIDFIAEFL 94

Query: 95  GLPLLPPYLSVGRRRISIQDFERGLNFAVAGATALDEAVLREKGITNLPTNYSLGVQMEW 154
           GLPL+PP+           +FE+G+NFAV GATAL+ + L ++GI    TN SLGVQ+  
Sbjct: 95  GLPLVPPFYGSHN-----ANFEKGVNFAVGGATALERSFLEDRGIHFPYTNVSLGVQLNS 154

Query: 155 FKNTYSSVCKSSFTATAKCRAILERSLIVVGEIGGNDYNYAFIDERQNIDEIKSLVAPVV 214
           FK +  S+C S     + CR ++E +LI++GEIGGNDYNYAF  ++  I+EIK L+  V+
Sbjct: 155 FKESLPSICGS----PSDCRDMIENALILMGEIGGNDYNYAFFVDK-GIEEIKELMPLVI 214

Query: 215 KAIGSAITELIKLGAQTLMVPGNLPIGCNPTYLKVYGTSVQNSEN---GCVNWLNEFSEY 274
             I SAITELI +G +T +VPG  P+GC+  YL  + TS     +   GC+ WLN+F E 
Sbjct: 215 TTISSAITELIGMGGRTFLVPGEFPVGCSVLYLTSHQTSNMEEYDPLTGCLKWLNKFGEN 274

Query: 275 HNEQLLQELKQTRALYPHIRIIYADYYNSAMLFYDAPQDFG 313
           H EQL  EL + + LYPH+ IIYADYYN+    Y  P  FG
Sbjct: 275 HGEQLRAELNRLQKLYPHVNIIYADYYNALFHLYQEPAKFG 305

BLAST of Carg15335 vs. TAIR 10
Match: AT1G28590.1 (GDSL-like Lipase/Acylhydrolase superfamily protein )

HSP 1 Score: 287.7 bits (735), Expect = 1.3e-77
Identity = 155/344 (45.06%), Postives = 215/344 (62.50%), Query Frame = 0

Query: 1   MGSSNSSPVQSLNRLFIVLVLSLLTFAPPPVLGCFTSIFNFGDSLSDTGNLFGNCVSEEP 60
           M S +S P   L R FI+  L + +         F SI +FGDS++DTGNL G     + 
Sbjct: 1   MASLDSLPAMKLVR-FILSTLLVTSVNSQTQCRNFKSIISFGDSIADTGNLLGLSDPNDL 60

Query: 61  PHSCFPPYGDAFFHHPTGRFSNGRLIVDFIAQSLGLPLLPPYLSVGRRRISIQDFERGLN 120
           P S FPPYG+ FFHHPTGR+S+GRLI+DFIA+ LG PL+PP+           +F++G+N
Sbjct: 61  PASAFPPYGETFFHHPTGRYSDGRLIIDFIAEFLGFPLVPPFYGCQN-----ANFKKGVN 120

Query: 121 FAVAGATALDEAVLREKGITNLPTNYSLGVQMEWFKNTYSSVCKSSFTATAKCRAILERS 180
           FAVAGATAL+ + L E+GI +  TN SL VQ+  F  +  ++C S     + CR ++E +
Sbjct: 121 FAVAGATALEPSFLEERGIHSTITNVSLSVQLRSFTESLPNLCGS----PSDCRDMIENA 180

Query: 181 LIVVGEIGGNDYNYAFIDERQNIDEIKSLVAPVVKAIGSAITELIKLGAQTLMVPGNLPI 240
           LI++GEIGGNDYN+A   +R+ + E++ LV  V+  I SAITEL+ +G +T +VPGN PI
Sbjct: 181 LILMGEIGGNDYNFALF-QRKPVKEVEELVPFVIATISSAITELVCMGGRTFLVPGNFPI 240

Query: 241 GCNPTYLKVYGTSVQNSEN---GCVNWLNEFSEYHNEQLLQELKQTRALYPHIRIIYADY 300
           G + +YL +Y TS +   +   GC+ WLN+FSEY+N+QL +EL   R LYPH+ IIYADY
Sbjct: 241 GYSASYLTLYKTSNKEEYDPLTGCLKWLNDFSEYYNKQLQEELNGLRKLYPHVNIIYADY 300

Query: 301 YNSAMLFYDAPQDFGREVSIPLPEIPLPGMFCGISQSTHMGAGR 342
           YN+ +  +  P  FG          PLP   CG+  S +    R
Sbjct: 301 YNALLRLFQEPAKFG------FMNRPLPAC-CGVGGSYNFNFSR 326

BLAST of Carg15335 vs. TAIR 10
Match: AT1G28570.1 (SGNH hydrolase-type esterase superfamily protein )

HSP 1 Score: 286.2 bits (731), Expect = 3.7e-77
Identity = 153/307 (49.84%), Postives = 197/307 (64.17%), Query Frame = 0

Query: 17  IVLVLSLLTFAPPPVLGCFTSIFNFGDSLSDTGNLFGNCVSEEPPHSCFPPYGDAFFHHP 76
           I+    L T    P    F SI +FGDS++DTGNL         P   F PYG+ FFHHP
Sbjct: 14  ILSTFCLTTVNSEPQCHNFKSIISFGDSIADTGNLLALSDPTNLPKVAFLPYGETFFHHP 73

Query: 77  TGRFSNGRLIVDFIAQSLGLPLLPPYLSVGRRRISIQDFERGLNFAVAGATALDEAVLRE 136
           TGRFSNGRLI+DFIA+ LG PL+PP+           +FE+G+NFAV GATAL+ + L E
Sbjct: 74  TGRFSNGRLIIDFIAEFLGFPLVPPFYGSQN-----ANFEKGVNFAVGGATALERSFLEE 133

Query: 137 KGITNLPTNYSLGVQMEWFKNTYSSVCKSSFTATAKCRAILERSLIVVGEIGGNDYNYAF 196
           +GI    TN SL VQ+  FK +  ++C S     + CR ++E SLI++GEIGGNDYNYAF
Sbjct: 134 RGIHFPYTNVSLAVQLSSFKESLPNLCVS----PSDCRDMIENSLILMGEIGGNDYNYAF 193

Query: 197 IDERQNIDEIKSLVAPVVKAIGSAITELIKLGAQTLMVPGNLPIGCNPTYLKVYGTS--- 256
               +NI+EIK LV  V++ I SAITELI +G +T +VPG  P+GC+  YL +Y TS   
Sbjct: 194 F-VGKNIEEIKELVPLVIETISSAITELIGMGGKTFLVPGEFPLGCSVAYLSLYQTSNIE 253

Query: 257 VQNSENGCVNWLNEFSEYHNEQLLQELKQTRALYPHIRIIYADYYNSAMLFYDAPQDFGR 316
             +   GC+ WLN+FSEYH+EQL  EL + + LYPH+ IIYADYYN+ +     P  FG 
Sbjct: 254 EYDPLTGCLKWLNKFSEYHDEQLQAELNRLQKLYPHVNIIYADYYNTLLRLAQEPAKFG- 309

Query: 317 EVSIPLP 321
            +S PLP
Sbjct: 314 FISRPLP 309

BLAST of Carg15335 vs. TAIR 10
Match: AT1G28650.1 (GDSL-like Lipase/Acylhydrolase superfamily protein )

HSP 1 Score: 278.1 bits (710), Expect = 1.0e-74
Identity = 143/280 (51.07%), Postives = 190/280 (67.86%), Query Frame = 0

Query: 35  FTSIFNFGDSLSDTGNLFGNCVSEEPPHSCFPPYGDAFFHHPTGRFSNGRLIVDFIAQSL 94
           + SI +FGDS++DTGN          P + F PYG++FFH P+GR+S+GRL++DFIA+ L
Sbjct: 35  YKSIISFGDSIADTGNYVHLSNVNNLPQAAFLPYGESFFHPPSGRYSDGRLVIDFIAEFL 94

Query: 95  GLPLLPPYLSVGRRRISIQDFERGLNFAVAGATALDEAVLREKGITNLPTNYSLGVQMEW 154
           GLP +PPY   G + +S   F +G+NFAV GATALD A L ++GI +  TN SL VQ+  
Sbjct: 95  GLPYVPPYF--GSQNVS---FNQGINFAVYGATALDRAFLVKQGIKSDFTNISLSVQLNT 154

Query: 155 FKNTYSSVCKSSFTATAKCRAILERSLIVVGEIGGNDYNYAFIDERQNIDEIKSLVAPVV 214
           FK    ++C SS   T  CR +L  SLI++GEIGGNDYNY F  E ++I+EIK LV  ++
Sbjct: 155 FKQILPNLCASS---TRDCREMLGDSLILMGEIGGNDYNYPFF-EGKSINEIKELVPLII 214

Query: 215 KAIGSAITELIKLGAQTLMVPGNLPIGCNPTYLKVYGTSVQNSE--NGCVNWLNEFSEYH 274
           KAI SAI +LI LG +T +VPGN PIGC+  YL ++ T+    +   GC+ WLN+F E+H
Sbjct: 215 KAISSAIVDLIDLGGKTFLVPGNFPIGCSTAYLTLFQTATVEHDPFTGCIPWLNKFGEHH 274

Query: 275 NEQLLQELKQTRALYPHIRIIYADYYNSAMLFYDAPQDFG 313
           NEQL  ELKQ + LYPH+ IIYADYYNS    +  P  +G
Sbjct: 275 NEQLKIELKQLQKLYPHVNIIYADYYNSLYGLFQEPAKYG 305