Carg15331 (gene) Silver-seed gourd (SMH-JMG-627) v2

Overview
NameCarg15331
Typegene
OrganismCucurbita argyrosperma subsp. argyrosperma cv. SMH-JMG-627 (Silver-seed gourd (SMH-JMG-627) v2)
DescriptionHXXXD-type acyl-transferase family protein
LocationCarg_Chr03: 9322897 .. 9324549 (-)
RNA-Seq ExpressionCarg15331
SyntenyCarg15331
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideexonCDSthree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGCTCCACGTCGCCCCCAATTACCACACACCAAACGGCAACGTACCCTCCACCAACCCCGCCAAGCGAGAGTCTACAAAATCAAGACCAAAATGCACCATTTGCATCCCCACCGGCCCACCACCTCACCGCCGTTCGAACAGCACAGCCATGCGAGTCACAGTATCAGCAACCACTCCCATTCTTCCTTCCCATCCCGCCTTCGCTCACGGCCATGTCCTCCCTCTTTCTCATCTCGACACAGATCCCAATCTCCGTGTCAGTTTCCGCTACCTTCGGGCCTATGTCTCCACCAAAGCCCACCAATCTCCTACCCCCGATCCCGTCGACGTCATTGCTACTGCCCTCTCCTCCGCCCTCCTCCATTACTACCCACTCGCTGGAACTCTCCGCCGTCAGAATCACCGCCTCGAGCTATTCTGTACTCACGGCCAAGGCGTCCCTCTCATCCGCGCCACCGCGGACTGTGAACTCGCGTCGGTTGATTACCTCGATAACCCGGACGAAACTTTTGTGGAGCAGTTGGTGCCCGATCCGGACGAGAATGAGGGGCTGAACCACCCTTGTATTCTACAAGTTACTCTGTTCGAATGCGGTGGGTTCACTCTCGGGGCTTCCATTCATCATTCTGTGTGCGACGGACTTGGGGCAACTCAGTTTTTCAATGCTGTGGCTGAATTAGCCCGTGGGGCGAGGACGGTGTCGATCGAACCAGTCTGGGACAGGGCTAGTTTGTTGGGCCCGAGGAATCCGCCCCGAATTGAGGTACCAATCGCAGATTTTTTGAGTTTAGAACATGGAAATTTACCCTACAGTCAAGAAGTTGGGGCGGTTGTGAGAGAATGCTTCCCTGTTACGGACGATCAACTGGAGAGGTTCAAGAGCATGTTGTTCAAACAATCTGGCTCTCGATTCACCGCGTTTGAAGCCCTGGGTGCATATATATGGCGCGCCAAGTGAGTCGAATTCTACCATGTCTGTTAATTTTGTGTTCCTGGTCTTTTATTGTTCTAACTTTTGGGGCTTCCTTCCATTCCCAGAGTGAAAGCCTCGGAAATTGCAGGCAATGAGAAGGTGAAATTCGTGTATTCGACAAACATACGGAAACAACTAAAGCCGCCATTACCGGTGGGTTACTATGGCAATGGTTGCGTTCCAATTTACGTTACACTCACGGCGGAGGAGATCAGAGAGCAACCCATTTGGGAGACGGCGAAGAAGATCCAGAAGAGCAAGATCAATATAAGCGACGAGTATGCACGATCTTTCATTGACTTTCAAGAGATTCATTGTGGGGAGGGAATCACTTCGGGGAAAGATGTGAGTGCGTTCACAGACTGGAGACATTTGGGGCATTCGACGGTGGATTTCGGTTGGGGAGGTCCGGCTACGGTGCTGCCACTTTCTCGTTTCCTTCTTGGGAGTGTGGAGCCTTGCTTCTTCTTGCCTCATTCTTCTGCAACTTCGAGCACGGCTGGGTTTAAGGTCTCCGTCGCTTTGAGGAAACCGGCGATGCCCTGTTTCAGAGAGGAGATGAAGAAGTTTGGTGATGATGATTTCGGGGTTATTGAGCTAAATTCGCTTCTCTAGTACCCAAATTTGGATCAAATTATATATGAATGTTTAAATTGTCTATTTGTTCTTGGAAGTTCT

mRNA sequence

ATGCTCCACGTCGCCCCCAATTACCACACACCAAACGGCAACGTACCCTCCACCAACCCCGCCAAGCGAGAGTCTACAAAATCAAGACCAAAATGCACCATTTGCATCCCCACCGGCCCACCACCTCACCGCCGTTCGAACAGCACAGCCATGCGAGTCACAGTATCAGCAACCACTCCCATTCTTCCTTCCCATCCCGCCTTCGCTCACGGCCATGTCCTCCCTCTTTCTCATCTCGACACAGATCCCAATCTCCGTGTCAGTTTCCGCTACCTTCGGGCCTATGTCTCCACCAAAGCCCACCAATCTCCTACCCCCGATCCCGTCGACGTCATTGCTACTGCCCTCTCCTCCGCCCTCCTCCATTACTACCCACTCGCTGGAACTCTCCGCCGTCAGAATCACCGCCTCGAGCTATTCTGTACTCACGGCCAAGGCGTCCCTCTCATCCGCGCCACCGCGGACTGTGAACTCGCGTCGGTTGATTACCTCGATAACCCGGACGAAACTTTTGTGGAGCAGTTGGTGCCCGATCCGGACGAGAATGAGGGGCTGAACCACCCTTGTATTCTACAAGTTACTCTGTTCGAATGCGGTGGGTTCACTCTCGGGGCTTCCATTCATCATTCTGTGTGCGACGGACTTGGGGCAACTCAGTTTTTCAATGCTGTGGCTGAATTAGCCCGTGGGGCGAGGACGGTGTCGATCGAACCAGTCTGGGACAGGGCTAGTTTGTTGGGCCCGAGGAATCCGCCCCGAATTGAGGTACCAATCGCAGATTTTTTGAGTTTAGAACATGGAAATTTACCCTACAGTCAAGAAGTTGGGGCGGTTGTGAGAGAATGCTTCCCTGTTACGGACGATCAACTGGAGAGGTTCAAGAGCATGTTGTTCAAACAATCTGGCTCTCGATTCACCGCGTTTGAAGCCCTGGGTGCATATATATGGCGCGCCAAAGTGAAAGCCTCGGAAATTGCAGGCAATGAGAAGGTGAAATTCGTGTATTCGACAAACATACGGAAACAACTAAAGCCGCCATTACCGGTGGGTTACTATGGCAATGGTTGCGTTCCAATTTACGTTACACTCACGGCGGAGGAGATCAGAGAGCAACCCATTTGGGAGACGGCGAAGAAGATCCAGAAGAGCAAGATCAATATAAGCGACGAGTATGCACGATCTTTCATTGACTTTCAAGAGATTCATTGTGGGGAGGGAATCACTTCGGGGAAAGATGTGAGTGCGTTCACAGACTGGAGACATTTGGGGCATTCGACGGTGGATTTCGGTTGGGGAGGTCCGGCTACGGTGCTGCCACTTTCTCGTTTCCTTCTTGGGAGTGTGGAGCCTTGCTTCTTCTTGCCTCATTCTTCTGCAACTTCGAGCACGGCTGGGTTTAAGGTCTCCGTCGCTTTGAGGAAACCGGCGATGCCCTGTTTCAGAGAGGAGATGAAGAAGTTTGGTGATGATGATTTCGGGGTTATTGAGCTAAATTCGCTTCTCTAGTACCCAAATTTGGATCAAATTATATATGAATGTTTAAATTGTCTATTTGTTCTTGGAAGTTCT

Coding sequence (CDS)

ATGCTCCACGTCGCCCCCAATTACCACACACCAAACGGCAACGTACCCTCCACCAACCCCGCCAAGCGAGAGTCTACAAAATCAAGACCAAAATGCACCATTTGCATCCCCACCGGCCCACCACCTCACCGCCGTTCGAACAGCACAGCCATGCGAGTCACAGTATCAGCAACCACTCCCATTCTTCCTTCCCATCCCGCCTTCGCTCACGGCCATGTCCTCCCTCTTTCTCATCTCGACACAGATCCCAATCTCCGTGTCAGTTTCCGCTACCTTCGGGCCTATGTCTCCACCAAAGCCCACCAATCTCCTACCCCCGATCCCGTCGACGTCATTGCTACTGCCCTCTCCTCCGCCCTCCTCCATTACTACCCACTCGCTGGAACTCTCCGCCGTCAGAATCACCGCCTCGAGCTATTCTGTACTCACGGCCAAGGCGTCCCTCTCATCCGCGCCACCGCGGACTGTGAACTCGCGTCGGTTGATTACCTCGATAACCCGGACGAAACTTTTGTGGAGCAGTTGGTGCCCGATCCGGACGAGAATGAGGGGCTGAACCACCCTTGTATTCTACAAGTTACTCTGTTCGAATGCGGTGGGTTCACTCTCGGGGCTTCCATTCATCATTCTGTGTGCGACGGACTTGGGGCAACTCAGTTTTTCAATGCTGTGGCTGAATTAGCCCGTGGGGCGAGGACGGTGTCGATCGAACCAGTCTGGGACAGGGCTAGTTTGTTGGGCCCGAGGAATCCGCCCCGAATTGAGGTACCAATCGCAGATTTTTTGAGTTTAGAACATGGAAATTTACCCTACAGTCAAGAAGTTGGGGCGGTTGTGAGAGAATGCTTCCCTGTTACGGACGATCAACTGGAGAGGTTCAAGAGCATGTTGTTCAAACAATCTGGCTCTCGATTCACCGCGTTTGAAGCCCTGGGTGCATATATATGGCGCGCCAAAGTGAAAGCCTCGGAAATTGCAGGCAATGAGAAGGTGAAATTCGTGTATTCGACAAACATACGGAAACAACTAAAGCCGCCATTACCGGTGGGTTACTATGGCAATGGTTGCGTTCCAATTTACGTTACACTCACGGCGGAGGAGATCAGAGAGCAACCCATTTGGGAGACGGCGAAGAAGATCCAGAAGAGCAAGATCAATATAAGCGACGAGTATGCACGATCTTTCATTGACTTTCAAGAGATTCATTGTGGGGAGGGAATCACTTCGGGGAAAGATGTGAGTGCGTTCACAGACTGGAGACATTTGGGGCATTCGACGGTGGATTTCGGTTGGGGAGGTCCGGCTACGGTGCTGCCACTTTCTCGTTTCCTTCTTGGGAGTGTGGAGCCTTGCTTCTTCTTGCCTCATTCTTCTGCAACTTCGAGCACGGCTGGGTTTAAGGTCTCCGTCGCTTTGAGGAAACCGGCGATGCCCTGTTTCAGAGAGGAGATGAAGAAGTTTGGTGATGATGATTTCGGGGTTATTGAGCTAAATTCGCTTCTCTAG

Protein sequence

MLHVAPNYHTPNGNVPSTNPAKRESTKSRPKCTICIPTGPPPHRRSNSTAMRVTVSATTPILPSHPAFAHGHVLPLSHLDTDPNLRVSFRYLRAYVSTKAHQSPTPDPVDVIATALSSALLHYYPLAGTLRRQNHRLELFCTHGQGVPLIRATADCELASVDYLDNPDETFVEQLVPDPDENEGLNHPCILQVTLFECGGFTLGASIHHSVCDGLGATQFFNAVAELARGARTVSIEPVWDRASLLGPRNPPRIEVPIADFLSLEHGNLPYSQEVGAVVRECFPVTDDQLERFKSMLFKQSGSRFTAFEALGAYIWRAKVKASEIAGNEKVKFVYSTNIRKQLKPPLPVGYYGNGCVPIYVTLTAEEIREQPIWETAKKIQKSKINISDEYARSFIDFQEIHCGEGITSGKDVSAFTDWRHLGHSTVDFGWGGPATVLPLSRFLLGSVEPCFFLPHSSATSSTAGFKVSVALRKPAMPCFREEMKKFGDDDFGVIELNSLL
Homology
BLAST of Carg15331 vs. NCBI nr
Match: KAG7034631.1 (Spermidine coumaroyl-CoA acyltransferase, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 1038.1 bits (2683), Expect = 2.5e-299
Identity = 501/501 (100.00%), Postives = 501/501 (100.00%), Query Frame = 0

Query: 1   MLHVAPNYHTPNGNVPSTNPAKRESTKSRPKCTICIPTGPPPHRRSNSTAMRVTVSATTP 60
           MLHVAPNYHTPNGNVPSTNPAKRESTKSRPKCTICIPTGPPPHRRSNSTAMRVTVSATTP
Sbjct: 1   MLHVAPNYHTPNGNVPSTNPAKRESTKSRPKCTICIPTGPPPHRRSNSTAMRVTVSATTP 60

Query: 61  ILPSHPAFAHGHVLPLSHLDTDPNLRVSFRYLRAYVSTKAHQSPTPDPVDVIATALSSAL 120
           ILPSHPAFAHGHVLPLSHLDTDPNLRVSFRYLRAYVSTKAHQSPTPDPVDVIATALSSAL
Sbjct: 61  ILPSHPAFAHGHVLPLSHLDTDPNLRVSFRYLRAYVSTKAHQSPTPDPVDVIATALSSAL 120

Query: 121 LHYYPLAGTLRRQNHRLELFCTHGQGVPLIRATADCELASVDYLDNPDETFVEQLVPDPD 180
           LHYYPLAGTLRRQNHRLELFCTHGQGVPLIRATADCELASVDYLDNPDETFVEQLVPDPD
Sbjct: 121 LHYYPLAGTLRRQNHRLELFCTHGQGVPLIRATADCELASVDYLDNPDETFVEQLVPDPD 180

Query: 181 ENEGLNHPCILQVTLFECGGFTLGASIHHSVCDGLGATQFFNAVAELARGARTVSIEPVW 240
           ENEGLNHPCILQVTLFECGGFTLGASIHHSVCDGLGATQFFNAVAELARGARTVSIEPVW
Sbjct: 181 ENEGLNHPCILQVTLFECGGFTLGASIHHSVCDGLGATQFFNAVAELARGARTVSIEPVW 240

Query: 241 DRASLLGPRNPPRIEVPIADFLSLEHGNLPYSQEVGAVVRECFPVTDDQLERFKSMLFKQ 300
           DRASLLGPRNPPRIEVPIADFLSLEHGNLPYSQEVGAVVRECFPVTDDQLERFKSMLFKQ
Sbjct: 241 DRASLLGPRNPPRIEVPIADFLSLEHGNLPYSQEVGAVVRECFPVTDDQLERFKSMLFKQ 300

Query: 301 SGSRFTAFEALGAYIWRAKVKASEIAGNEKVKFVYSTNIRKQLKPPLPVGYYGNGCVPIY 360
           SGSRFTAFEALGAYIWRAKVKASEIAGNEKVKFVYSTNIRKQLKPPLPVGYYGNGCVPIY
Sbjct: 301 SGSRFTAFEALGAYIWRAKVKASEIAGNEKVKFVYSTNIRKQLKPPLPVGYYGNGCVPIY 360

Query: 361 VTLTAEEIREQPIWETAKKIQKSKINISDEYARSFIDFQEIHCGEGITSGKDVSAFTDWR 420
           VTLTAEEIREQPIWETAKKIQKSKINISDEYARSFIDFQEIHCGEGITSGKDVSAFTDWR
Sbjct: 361 VTLTAEEIREQPIWETAKKIQKSKINISDEYARSFIDFQEIHCGEGITSGKDVSAFTDWR 420

Query: 421 HLGHSTVDFGWGGPATVLPLSRFLLGSVEPCFFLPHSSATSSTAGFKVSVALRKPAMPCF 480
           HLGHSTVDFGWGGPATVLPLSRFLLGSVEPCFFLPHSSATSSTAGFKVSVALRKPAMPCF
Sbjct: 421 HLGHSTVDFGWGGPATVLPLSRFLLGSVEPCFFLPHSSATSSTAGFKVSVALRKPAMPCF 480

Query: 481 REEMKKFGDDDFGVIELNSLL 502
           REEMKKFGDDDFGVIELNSLL
Sbjct: 481 REEMKKFGDDDFGVIELNSLL 501

BLAST of Carg15331 vs. NCBI nr
Match: KAG6581346.1 (Spermidine coumaroyl-CoA acyltransferase, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 1030.8 bits (2664), Expect = 3.9e-297
Identity = 497/501 (99.20%), Postives = 498/501 (99.40%), Query Frame = 0

Query: 1   MLHVAPNYHTPNGNVPSTNPAKRESTKSRPKCTICIPTGPPPHRRSNSTAMRVTVSATTP 60
           MLHVAPNYHTPNGNVPSTNPAKRESTKSRPKCTICIPTGPPPHRRSNSTAMRVTVSATTP
Sbjct: 1   MLHVAPNYHTPNGNVPSTNPAKRESTKSRPKCTICIPTGPPPHRRSNSTAMRVTVSATTP 60

Query: 61  ILPSHPAFAHGHVLPLSHLDTDPNLRVSFRYLRAYVSTKAHQSPTPDPVDVIATALSSAL 120
           ILPSHPAFAHGHVLPLSHLDTDPNLRVSFRYLRAYVST AHQSPTPDPVDVIATALSSAL
Sbjct: 61  ILPSHPAFAHGHVLPLSHLDTDPNLRVSFRYLRAYVSTNAHQSPTPDPVDVIATALSSAL 120

Query: 121 LHYYPLAGTLRRQNHRLELFCTHGQGVPLIRATADCELASVDYLDNPDETFVEQLVPDPD 180
           LHYYPLAGTLRRQNHRLELFCTHGQGVPLIRATADCELASVDYLDNPDETFVEQLVPDPD
Sbjct: 121 LHYYPLAGTLRRQNHRLELFCTHGQGVPLIRATADCELASVDYLDNPDETFVEQLVPDPD 180

Query: 181 ENEGLNHPCILQVTLFECGGFTLGASIHHSVCDGLGATQFFNAVAELARGARTVSIEPVW 240
           ENEGLNHPCILQVTLFECGGFTLGASIHHSVCDGLGATQFFNAVAELARGARTVSIEPVW
Sbjct: 181 ENEGLNHPCILQVTLFECGGFTLGASIHHSVCDGLGATQFFNAVAELARGARTVSIEPVW 240

Query: 241 DRASLLGPRNPPRIEVPIADFLSLEHGNLPYSQEVGAVVRECFPVTDDQLERFKSMLFKQ 300
           DRASLLGPRNPPRIEVPIADFLSLEHGNLPYSQEVGAVVRECFPVTDDQLERFKSMLFKQ
Sbjct: 241 DRASLLGPRNPPRIEVPIADFLSLEHGNLPYSQEVGAVVRECFPVTDDQLERFKSMLFKQ 300

Query: 301 SGSRFTAFEALGAYIWRAKVKASEIAGNEKVKFVYSTNIRKQLKPPLPVGYYGNGCVPIY 360
           SGSRFTAFEALGAYIWRAKVKASEIAGNEKVKFVYSTNIRKQLKPPLPVGYYGNGCVPIY
Sbjct: 301 SGSRFTAFEALGAYIWRAKVKASEIAGNEKVKFVYSTNIRKQLKPPLPVGYYGNGCVPIY 360

Query: 361 VTLTAEEIREQPIWETAKKIQKSKINISDEYARSFIDFQEIHCGEGITSGKDVSAFTDWR 420
           VTLTAEEIREQPIWETAKKIQKSKINISDEY RSFIDFQEIHCGEGITSGKDVSAFTDWR
Sbjct: 361 VTLTAEEIREQPIWETAKKIQKSKINISDEYVRSFIDFQEIHCGEGITSGKDVSAFTDWR 420

Query: 421 HLGHSTVDFGWGGPATVLPLSRFLLGSVEPCFFLPHSSATSSTAGFKVSVALRKPAMPCF 480
           HLGHSTVDFGWGGPATVLPLSRFLLGSVE CFFLPHSSATSSTAGFKVSVALRKPAMPCF
Sbjct: 421 HLGHSTVDFGWGGPATVLPLSRFLLGSVERCFFLPHSSATSSTAGFKVSVALRKPAMPCF 480

Query: 481 REEMKKFGDDDFGVIELNSLL 502
           REEMKKFGDDDFG+IELNSLL
Sbjct: 481 REEMKKFGDDDFGIIELNSLL 501

BLAST of Carg15331 vs. NCBI nr
Match: XP_022925827.1 (spermidine coumaroyl-CoA acyltransferase [Cucurbita moschata])

HSP 1 Score: 932.2 bits (2408), Expect = 1.9e-267
Identity = 450/451 (99.78%), Postives = 450/451 (99.78%), Query Frame = 0

Query: 51  MRVTVSATTPILPSHPAFAHGHVLPLSHLDTDPNLRVSFRYLRAYVSTKAHQSPTPDPVD 110
           MRVTVSATTPILPSHPAFAHGHVLPLSHLDTDPNLRVSFRYLRAYVST AHQSPTPDPVD
Sbjct: 1   MRVTVSATTPILPSHPAFAHGHVLPLSHLDTDPNLRVSFRYLRAYVSTNAHQSPTPDPVD 60

Query: 111 VIATALSSALLHYYPLAGTLRRQNHRLELFCTHGQGVPLIRATADCELASVDYLDNPDET 170
           VIATALSSALLHYYPLAGTLRRQNHRLELFCTHGQGVPLIRATADCELASVDYLDNPDET
Sbjct: 61  VIATALSSALLHYYPLAGTLRRQNHRLELFCTHGQGVPLIRATADCELASVDYLDNPDET 120

Query: 171 FVEQLVPDPDENEGLNHPCILQVTLFECGGFTLGASIHHSVCDGLGATQFFNAVAELARG 230
           FVEQLVPDPDENEGLNHPCILQVTLFECGGFTLGASIHHSVCDGLGATQFFNAVAELARG
Sbjct: 121 FVEQLVPDPDENEGLNHPCILQVTLFECGGFTLGASIHHSVCDGLGATQFFNAVAELARG 180

Query: 231 ARTVSIEPVWDRASLLGPRNPPRIEVPIADFLSLEHGNLPYSQEVGAVVRECFPVTDDQL 290
           ARTVSIEPVWDRASLLGPRNPPRIEVPIADFLSLEHGNLPYSQEVGAVVRECFPVTDDQL
Sbjct: 181 ARTVSIEPVWDRASLLGPRNPPRIEVPIADFLSLEHGNLPYSQEVGAVVRECFPVTDDQL 240

Query: 291 ERFKSMLFKQSGSRFTAFEALGAYIWRAKVKASEIAGNEKVKFVYSTNIRKQLKPPLPVG 350
           ERFKSMLFKQSGSRFTAFEALGAYIWRAKVKASEIAGNEKVKFVYSTNIRKQLKPPLPVG
Sbjct: 241 ERFKSMLFKQSGSRFTAFEALGAYIWRAKVKASEIAGNEKVKFVYSTNIRKQLKPPLPVG 300

Query: 351 YYGNGCVPIYVTLTAEEIREQPIWETAKKIQKSKINISDEYARSFIDFQEIHCGEGITSG 410
           YYGNGCVPIYVTLTAEEIREQPIWETAKKIQKSKINISDEYARSFIDFQEIHCGEGITSG
Sbjct: 301 YYGNGCVPIYVTLTAEEIREQPIWETAKKIQKSKINISDEYARSFIDFQEIHCGEGITSG 360

Query: 411 KDVSAFTDWRHLGHSTVDFGWGGPATVLPLSRFLLGSVEPCFFLPHSSATSSTAGFKVSV 470
           KDVSAFTDWRHLGHSTVDFGWGGPATVLPLSRFLLGSVEPCFFLPHSSATSSTAGFKVSV
Sbjct: 361 KDVSAFTDWRHLGHSTVDFGWGGPATVLPLSRFLLGSVEPCFFLPHSSATSSTAGFKVSV 420

Query: 471 ALRKPAMPCFREEMKKFGDDDFGVIELNSLL 502
           ALRKPAMPCFREEMKKFGDDDFGVIELNSLL
Sbjct: 421 ALRKPAMPCFREEMKKFGDDDFGVIELNSLL 451

BLAST of Carg15331 vs. NCBI nr
Match: XP_023543910.1 (spermidine coumaroyl-CoA acyltransferase [Cucurbita pepo subsp. pepo])

HSP 1 Score: 916.0 bits (2366), Expect = 1.4e-262
Identity = 439/451 (97.34%), Postives = 444/451 (98.45%), Query Frame = 0

Query: 51  MRVTVSATTPILPSHPAFAHGHVLPLSHLDTDPNLRVSFRYLRAYVSTKAHQSPTPDPVD 110
           MRVTVSATTPILPSHPAFAH HVLPLSHLD DPNLRVSFRYLRAYVST AHQSPTPDPVD
Sbjct: 1   MRVTVSATTPILPSHPAFAHSHVLPLSHLDIDPNLRVSFRYLRAYVSTNAHQSPTPDPVD 60

Query: 111 VIATALSSALLHYYPLAGTLRRQNHRLELFCTHGQGVPLIRATADCELASVDYLDNPDET 170
           VIATALSSALLHYYPLAGTLRRQNHRLE+FC HGQGVPLIRATADCELASVDYLDNPDET
Sbjct: 61  VIATALSSALLHYYPLAGTLRRQNHRLEMFCAHGQGVPLIRATADCELASVDYLDNPDET 120

Query: 171 FVEQLVPDPDENEGLNHPCILQVTLFECGGFTLGASIHHSVCDGLGATQFFNAVAELARG 230
           FVEQLVPDPDENEGLNHPCILQVTLFECGGFTLGASIHHSVCDGLGATQFFNAVAELARG
Sbjct: 121 FVEQLVPDPDENEGLNHPCILQVTLFECGGFTLGASIHHSVCDGLGATQFFNAVAELARG 180

Query: 231 ARTVSIEPVWDRASLLGPRNPPRIEVPIADFLSLEHGNLPYSQEVGAVVRECFPVTDDQL 290
           ARTVSIEPVWDRASLLGPRNPPRIEVPIADFLSLEHGNLPYSQEVGAVVRECFPVTDDQL
Sbjct: 181 ARTVSIEPVWDRASLLGPRNPPRIEVPIADFLSLEHGNLPYSQEVGAVVRECFPVTDDQL 240

Query: 291 ERFKSMLFKQSGSRFTAFEALGAYIWRAKVKASEIAGNEKVKFVYSTNIRKQLKPPLPVG 350
           ERFKSMLFKQSGSRFTAFEALGAYIWRAKVKASEIAGNEKVKFVYSTNIRKQLKPPLPVG
Sbjct: 241 ERFKSMLFKQSGSRFTAFEALGAYIWRAKVKASEIAGNEKVKFVYSTNIRKQLKPPLPVG 300

Query: 351 YYGNGCVPIYVTLTAEEIREQPIWETAKKIQKSKINISDEYARSFIDFQEIHCGEGITSG 410
           YYGNGCVPIYVTLTAEE+REQPIWETAKKIQKSKINISDEYARSFIDFQEIHCG+GIT G
Sbjct: 301 YYGNGCVPIYVTLTAEEVREQPIWETAKKIQKSKINISDEYARSFIDFQEIHCGDGITPG 360

Query: 411 KDVSAFTDWRHLGHSTVDFGWGGPATVLPLSRFLLGSVEPCFFLPHSSATSSTAGFKVSV 470
           K+VSAFTDWRHLGHSTVDFGWGGP TVLPLSRFLLGSVEPCFFLPHSSATSS AGFKVSV
Sbjct: 361 KEVSAFTDWRHLGHSTVDFGWGGPVTVLPLSRFLLGSVEPCFFLPHSSATSSAAGFKVSV 420

Query: 471 ALRKPAMPCFREEMKKFGDDDFGVIELNSLL 502
           ALRKPAMPCFREEMKKFGDDDFGV+ELNSLL
Sbjct: 421 ALRKPAMPCFREEMKKFGDDDFGVVELNSLL 451

BLAST of Carg15331 vs. NCBI nr
Match: XP_022978699.1 (spermidine coumaroyl-CoA acyltransferase [Cucurbita maxima])

HSP 1 Score: 902.5 bits (2331), Expect = 1.6e-258
Identity = 434/451 (96.23%), Postives = 438/451 (97.12%), Query Frame = 0

Query: 51  MRVTVSATTPILPSHPAFAHGHVLPLSHLDTDPNLRVSFRYLRAYVSTKAHQSPTPDPVD 110
           MRVTVSATTPILPSHP FAH HVLPLSHLDTDPNLRVSFRYLRAYVST AHQSPTPDP D
Sbjct: 1   MRVTVSATTPILPSHPPFAHSHVLPLSHLDTDPNLRVSFRYLRAYVSTNAHQSPTPDPFD 60

Query: 111 VIATALSSALLHYYPLAGTLRRQNHRLELFCTHGQGVPLIRATADCELASVDYLDNPDET 170
           VIATALSSALLHYYPLAGTLR QNHRLELFC HGQGVPLIRATADCELASVDYLD PDET
Sbjct: 61  VIATALSSALLHYYPLAGTLRCQNHRLELFCAHGQGVPLIRATADCELASVDYLDKPDET 120

Query: 171 FVEQLVPDPDENEGLNHPCILQVTLFECGGFTLGASIHHSVCDGLGATQFFNAVAELARG 230
           FVEQLVPDPDENEGLNHPCILQVTLFECGGFTLGASIHHSVCDGLGATQFFNAVAELARG
Sbjct: 121 FVEQLVPDPDENEGLNHPCILQVTLFECGGFTLGASIHHSVCDGLGATQFFNAVAELARG 180

Query: 231 ARTVSIEPVWDRASLLGPRNPPRIEVPIADFLSLEHGNLPYSQEVGAVVRECFPVTDDQL 290
           ARTVSIEPVWDRASLLGPRNPPRIE+PIADFLSLEHGNLPYSQEVGAVVRECFPVTDDQL
Sbjct: 181 ARTVSIEPVWDRASLLGPRNPPRIELPIADFLSLEHGNLPYSQEVGAVVRECFPVTDDQL 240

Query: 291 ERFKSMLFKQSGSRFTAFEALGAYIWRAKVKASEIAGNEKVKFVYSTNIRKQLKPPLPVG 350
           ERFKSMLFKQSGSRFTAFEALGAYIWRAKVKASEIAGNEKVKFVYSTNIRKQLKPPLPVG
Sbjct: 241 ERFKSMLFKQSGSRFTAFEALGAYIWRAKVKASEIAGNEKVKFVYSTNIRKQLKPPLPVG 300

Query: 351 YYGNGCVPIYVTLTAEEIREQPIWETAKKIQKSKINISDEYARSFIDFQEIHCGEGITSG 410
           YYGNGCVPIYVTLTAEE+REQPIWETAKKIQKSKINISDEY RSFIDFQEIHCG+GIT G
Sbjct: 301 YYGNGCVPIYVTLTAEEVREQPIWETAKKIQKSKINISDEYVRSFIDFQEIHCGDGITPG 360

Query: 411 KDVSAFTDWRHLGHSTVDFGWGGPATVLPLSRFLLGSVEPCFFLPHSSATSSTAGFKVSV 470
           K+VSAFTDWRHLGHSTVDFGWGGP TVLPLSRFLLGSVEPCFFLPHSSAT S AGFKVSV
Sbjct: 361 KEVSAFTDWRHLGHSTVDFGWGGPVTVLPLSRFLLGSVEPCFFLPHSSATWSAAGFKVSV 420

Query: 471 ALRKPAMPCFREEMKKFGDDDFGVIELNSLL 502
           ALRKPAMPCFREEMKKFGDDDFGVIELN LL
Sbjct: 421 ALRKPAMPCFREEMKKFGDDDFGVIELNLLL 451

BLAST of Carg15331 vs. ExPASy Swiss-Prot
Match: Q8GYW8 (Spermidine coumaroyl-CoA acyltransferase OS=Arabidopsis thaliana OX=3702 GN=SCT PE=1 SV=1)

HSP 1 Score: 227.6 bits (579), Expect = 3.0e-58
Identity = 149/445 (33.48%), Postives = 223/445 (50.11%), Query Frame = 0

Query: 70  HGHVLPLSHLDTDPNLRVSFRYLRAYVSTKAHQSPTPDPVDVIATALSSALLHYYPLAGT 129
           H   L LS LD DP   V +  +  +   KA+     DPV ++  ALS  L+HYYPL+G 
Sbjct: 28  HCETLSLSTLDNDPFNEVMYATIYVF---KANGKNLDDPVSLLRKALSELLVHYYPLSGK 87

Query: 130 LRR--QNHRLELFCTHGQGVPLIRATADCELASVDYLDNPDETFVEQLVPDPD---ENEG 189
           L R   N +L+L    G+GVP   AT+  +L+S++Y++N D+    +LVP+ +   E+  
Sbjct: 88  LMRSESNGKLQLVYL-GEGVPFEVATSTLDLSSLNYIENLDDQVALRLVPEIEIDYESNV 147

Query: 190 LNHPCILQVTLFECGGFTLGASIHHSVCDGLGATQFFNAVAELARGARTVSIEPVWDRAS 249
             HP  LQVT F CGGFT+G ++ H+VCDG G  Q  +A+ ELA G    S++ VW R  
Sbjct: 148 CYHPLALQVTKFACGGFTIGTALTHAVCDGYGVAQIIHALTELAAGKTEPSVKSVWQRER 207

Query: 250 LLG--PRNPPRIEVPIADFLSLEHGNLPYSQEVGAVVRECFPVTDDQLERFKSMLFKQS- 309
           L+G     P ++     D        LP +     VV E   +    ++R K  + K+  
Sbjct: 208 LVGKIDNKPGKVPGSHIDGFLATSAYLPTTD----VVTETINIRAGDIKRLKDSMMKECE 267

Query: 310 --GSRFTAFEALGAYIWRAKVKASEIAGNEKVKFVYSTNIRKQLKPPLPVGYYGNGCVPI 369
                FT +E L +YIW+ + +A ++  +       +  IR  L PPLP GYYGN  + +
Sbjct: 268 YLKESFTTYEVLSSYIWKLRSRALKLNPDGITVLGVAVGIRHVLDPPLPKGYYGNAYIDV 327

Query: 370 YVTLTAEEIREQPIWETAKKIQKSKINISDEYARSFID---------FQEIHCGEGITSG 429
           YV LT  E+ E  I   A +++K+K      Y + +I+          ++    EG++ G
Sbjct: 328 YVELTVRELEESSISNIANRVKKAK---KTAYEKGYIEEELKNTERLMRDDSMFEGVSDG 387

Query: 430 KDVSAFTDWRHLG-HSTVDFGWGGPATVLPLSRFLLGSVEPCFFLPHSSATSSTAGFKVS 489
             +   TDWR++G   ++DFGW  P  + PL++            P  S  S   G KV 
Sbjct: 388 --LFFLTDWRNIGWFGSMDFGWNEPVNLRPLTQRESTVHVGMILKPSKSDPSMEGGVKVI 447

BLAST of Carg15331 vs. ExPASy Swiss-Prot
Match: O80467 (Spermidine sinapoyl-CoA acyltransferase OS=Arabidopsis thaliana OX=3702 GN=SDT PE=1 SV=1)

HSP 1 Score: 208.8 bits (530), Expect = 1.5e-52
Identity = 144/426 (33.80%), Postives = 211/426 (49.53%), Query Frame = 0

Query: 74  LPLSHLDTDPNLRVSFRYLRAYVSTKAHQSPTPD--PVDVIATALSSALLHYYPLAGTLR 133
           L LS LD DP   V ++    +   KA      D  P  ++  ALS  L +YYPL+G+L+
Sbjct: 32  LNLSTLDNDPYNEVIYKACYVF---KAKNVADDDNRPEALLREALSDLLGYYYPLSGSLK 91

Query: 134 RQ--NHRLELFC-THGQGVPLIRATADCELASVDYLDNPDETFVEQLVPDPDENEGLNHP 193
           RQ  + +L+L C   G GVP   ATA+ EL+S+  L+N D       +P    +     P
Sbjct: 92  RQESDRKLQLSCGGDGGGVPFTVATANVELSSLKNLENIDSDTALNFLPVLHVDIDGYRP 151

Query: 194 CILQVTLFECGGFTLGASIHHSVCDGLGATQFFNAVAELARGARTVSIEPVWDRASLLG- 253
             LQVT FECGGF LG ++ H++CDG G      A+ +LA G +   + P+W+R  L+G 
Sbjct: 152 FALQVTKFECGGFILGMAMSHAMCDGYGEGHIMCALTDLAGGKKKPMVTPIWERERLVGK 211

Query: 254 PRNPPRIEVPIADFLSLEHGNLPYSQEVGAVVRECFPVTDDQLERFKSMLFKQ---SGSR 313
           P +     VP  D  +  +  LP        V E   +  D + R K    K+   S   
Sbjct: 212 PEDDQPPFVPGDDTAASPY--LPTDD----WVTEKITIRADSIRRLKEATLKEYDFSNET 271

Query: 314 FTAFEALGAYIWRAKVKASEIAGNEKVKFVYSTNIRKQLKPPLPVGYYGNGCVPIYVTLT 373
            T FE +GAY+W+++VKA  +  +       S  IR  + PPLP GYYGN  + +YV LT
Sbjct: 272 ITTFEVIGAYLWKSRVKALNLDRDGVTVLGLSVGIRNVVDPPLPDGYYGNAYIDMYVPLT 331

Query: 374 AEEIREQPIWETAKKIQKSKINISD-EYARSFIDFQE--IHCGEGITSGKD-VSAFTDWR 433
           A E+ E  I +  K I+++K N  D +Y +  +   E  I     I   KD +   TDWR
Sbjct: 332 AREVEEFTISDIVKLIKEAKRNAHDKDYLQEELANTEKIIKMNLTIKGKKDGLFCLTDWR 391

Query: 434 HLG-HSTVDFGWGGPATVLPLSRFLLGSVEPCFFLPHSSATSSTAGFKVSVALRKPAMPC 486
           ++G   ++DFGW  P  ++P+           F  P    +    G ++ V L + AM  
Sbjct: 392 NIGIFGSMDFGWDEPVNIVPVVPSETARTVNMFMRPSRLESDMVGGVQIVVTLPRIAMVK 448

BLAST of Carg15331 vs. ExPASy Swiss-Prot
Match: Q8LL69 (3'-N-debenzoyl-2'-deoxytaxol N-benzoyltransferase OS=Taxus canadensis OX=88032 GN=TAX10 PE=1 SV=1)

HSP 1 Score: 206.8 bits (525), Expect = 5.6e-52
Identity = 140/420 (33.33%), Postives = 213/420 (50.71%), Query Frame = 0

Query: 60  PILPSHPAFAHGHVLPLSHLDTDPNLRVSFRYLRAYVSTKAHQSPTPDPVDVIATALSSA 119
           P LPS  A      + LS +D+    R  F  L  +    A  + + DPV +I  ALS  
Sbjct: 21  PSLPSPKA-----TVQLSVVDSLTICRGIFNTLLVF---NAPDNISADPVKIIREALSKV 80

Query: 120 LLHYYPLAGTLR-RQNHRLELFCTHGQGVPLIRATADCELASVDYLDNPDETFVEQLV-- 179
           L++Y+PLAG LR ++   LE+ CT G G   + A  +  ++ +  LD+ + +F +QLV  
Sbjct: 81  LVYYFPLAGRLRSKEIGELEVECT-GDGALFVEAMVEDTISVLRDLDDLNPSF-QQLVFW 140

Query: 180 -PDPDENEGLNHPCILQVTLFECGGFTLGASIHHSVCDGLGATQFFNAVAELARGARTVS 239
            P     E L H  I+QVT F CGG  +G ++ HSVCDG GA QF  A+AE+ARG    S
Sbjct: 141 HPLDTAIEDL-HLVIVQVTRFTCGGIAVGVTLPHSVCDGRGAAQFVTALAEMARGEVKPS 200

Query: 240 IEPVWDRASLLGPRNPPRIEVPIADFLSLEHGNLPYSQEVGAVVRECFPVTDDQLERFKS 299
           +EP+W+R  LL P +P  +++   D +       P  +E+G   +  F +  D +E  K 
Sbjct: 201 LEPIWNR-ELLNPEDPLHLQLNQFDSICPP----PMLEELG---QASFVINVDTIEYMKQ 260

Query: 300 MLFKQSGSRFTAFEALGAYIWRAKVKASEIAGNEKVKFVYSTNIRKQLKPPLPVGYYGNG 359
            + ++     ++FE + A +W A+ KA +I   E VK +++ ++RK   PPLP GYYGN 
Sbjct: 261 CVMEECNEFCSSFEVVAALVWIARTKALQIPHTENVKLLFAMDLRKLFNPPLPNGYYGNA 320

Query: 360 CVPIYVTLTAEEIREQPIWETAKKIQKSKINISDEYARSFIDFQEIHCGEGITSGKDVSA 419
               Y     +++    +      I+K+K ++ D Y+RS +            S  ++ A
Sbjct: 321 IGTAYAMDNVQDLLNGSLLRAIMIIKKAKADLKDNYSRSRVVTNPYSLDVNKKS-DNILA 380

Query: 420 FTDWRHLGHSTVDFGWGGPATVLPLSRFLLGSVEPCFFLPHSSATSSTAGFKVSVALRKP 476
            +DWR LG    DFGWGGP  V  L R   G      FL    A + + G K+ ++   P
Sbjct: 381 LSDWRRLGFYEADFGWGGPLNVSSLQRLENGLPMFSTFLYLLPAKNKSDGIKLLLSCMPP 420

BLAST of Carg15331 vs. ExPASy Swiss-Prot
Match: Q8S9G6 (Taxadien-5-alpha-ol O-acetyltransferase OS=Taxus wallichiana var. chinensis OX=29808 PE=2 SV=1)

HSP 1 Score: 200.7 bits (509), Expect = 4.0e-50
Identity = 134/441 (30.39%), Postives = 221/441 (50.11%), Query Frame = 0

Query: 49  TAMRVTVSATTPILPSHPAFAHGHVLPLSHLDTDPNLRVS-FRYLRAYVSTKAHQSPTPD 108
           T + V ++    + PS P       L LS +D  P +R S F  L  Y ++ +    + D
Sbjct: 4   TDLHVNLNEKVMVGPSLP--LPKTTLQLSSIDNLPGVRGSIFNALLIYNASPSPTMVSAD 63

Query: 109 PVDVIATALSSALLHYYPLAGTLRR-QNHRLELFCTHGQGVPLIRATADCELASVDYLDN 168
           P  +I  AL+  L++Y P AG LR  +N  LE+ CT G+G   + A AD EL+ +   D+
Sbjct: 64  PAKLIREALAKILVYYPPFAGRLRETENGDLEVECT-GEGAMFLEAMADNELSVLGDFDD 123

Query: 169 PDETFVEQLVPDPDENEGLNHP-CILQVTLFECGGFTLGASIHHSVCDGLGATQFFNAVA 228
            + +F + L   P +    + P  ++QVT F CGGF +G S HH VCDG GA QF   +A
Sbjct: 124 SNPSFQQLLFSLPLDTNFKDLPLLVVQVTRFTCGGFVVGVSFHHGVCDGRGAAQFLKGLA 183

Query: 229 ELARGARTVSIEPVWDRASLLGPRNPPRIEVPIADFLSLEHGNLPYSQEVGAVVRECFPV 288
           E+ARG   +S+EP+W+R  L+   +P  ++     F   E    P    V  +V+  F +
Sbjct: 184 EMARGEVKLSLEPIWNR-ELVKLDDPKYLQ-----FFHFEFLRAP--SIVEKIVQTYFII 243

Query: 289 TDDQLERFKSMLFKQSGSRFTAFEALGAYIWRAKVKASEIAGNEKVKFVYSTNIRKQLKP 348
             + +   K  + ++     ++FE   A  W A+ +A +I  +E VK ++  ++R    P
Sbjct: 244 DFETINYIKQSVMEECKEFCSSFEVASAMTWIARTRAFQIPESEYVKILFGMDMRNSFNP 303

Query: 349 PLPVGYYGNGCVPIYVTLTAEEIREQPIWETAKKIQKSKINISDEY-ARSFIDFQEIHCG 408
           PLP GYYGN           +++    +      I+KSK++++D + +R+ +   E+   
Sbjct: 304 PLPSGYYGNSIGTACAVDNVQDLLSGSLLRAIMIIKKSKVSLNDNFKSRAVVKPSELDVN 363

Query: 409 EGITSGKDVSAFTDWRHLGHSTVDFGWGGPATVLPLSRFLLGSVEPCFFLPHSSATSSTA 468
               + ++V AF DW  LG   VDFGWG   +V P+ +    +++  +FL    + +   
Sbjct: 364 ---MNHENVVAFADWSRLGFDEVDFGWGNAVSVSPVQQQCELAMQN-YFLFLKPSKNKPD 423

Query: 469 GFKVSVALRKPAMPCFREEMK 486
           G K+ + L    M  F+ EM+
Sbjct: 424 GIKILMFLPLSKMKSFKIEME 429

BLAST of Carg15331 vs. ExPASy Swiss-Prot
Match: Q9M6F0 (Taxadien-5-alpha-ol O-acetyltransferase OS=Taxus cuspidata OX=99806 GN=TAT PE=1 SV=1)

HSP 1 Score: 199.1 bits (505), Expect = 1.2e-49
Identity = 130/416 (31.25%), Postives = 210/416 (50.48%), Query Frame = 0

Query: 74  LPLSHLDTDPNLRVS-FRYLRAYVSTKAHQSPTPDPVDVIATALSSALLHYYPLAGTLRR 133
           L LS +D  P +R S F  L  Y ++ +    + DP   I  AL+  L++Y P AG LR 
Sbjct: 27  LQLSSIDNLPGVRGSIFNALLIYNASPSPTMISADPAKPIREALAKILVYYPPFAGRLRE 86

Query: 134 -QNHRLELFCTHGQGVPLIRATADCELASVDYLDNPDETFVEQLVPDP-DENEGLNHPCI 193
            +N  LE+ CT G+G   + A AD EL+ +   D+ + +F + L   P D N       +
Sbjct: 87  TENGDLEVECT-GEGAMFLEAMADNELSVLGDFDDSNPSFQQLLFSLPLDTNFKDLSLLV 146

Query: 194 LQVTLFECGGFTLGASIHHSVCDGLGATQFFNAVAELARGARTVSIEPVWDRASLLGPRN 253
           +QVT F CGGF +G S HH VCDG GA QF   +AE+ARG   +S+EP+W+R  L+   +
Sbjct: 147 VQVTRFTCGGFVVGVSFHHGVCDGRGAAQFLKGLAEMARGEVKLSLEPIWNR-ELVKLDD 206

Query: 254 PPRIEVPIADFLSLEHGNLPYSQEVGAVVRECFPVTDDQLERFKSMLFKQSGSRFTAFEA 313
           P  ++     F   E    P    V  +V+  F +  + +   K  + ++     ++FE 
Sbjct: 207 PKYLQ-----FFHFEFLRAP--SIVEKIVQTYFIIDFETINYIKQSVMEECKEFCSSFEV 266

Query: 314 LGAYIWRAKVKASEIAGNEKVKFVYSTNIRKQLKPPLPVGYYGNGCVPIYVTLTAEEIRE 373
             A  W A+ +A +I  +E VK ++  ++R    PPLP GYYGN           +++  
Sbjct: 267 ASAMTWIARTRAFQIPESEYVKILFGMDMRNSFNPPLPSGYYGNSIGTACAVDNVQDLLS 326

Query: 374 QPIWETAKKIQKSKINISDEY-ARSFIDFQEIHCGEGITSGKDVSAFTDWRHLGHSTVDF 433
             +      I+KSK++++D + +R+ +   E+       + ++V AF DW  LG   VDF
Sbjct: 327 GSLLRAIMIIKKSKVSLNDNFKSRAVVKPSELDVN---MNHENVVAFADWSRLGFDEVDF 386

Query: 434 GWGGPATVLPLSRFLLGSVEPCFFLPHSSATSSTAGFKVSVALRKPAMPCFREEMK 486
           GWG   +V P+ +    +++  +FL    + +   G K+ + L    M  F+ EM+
Sbjct: 387 GWGNAVSVSPVQQQSALAMQN-YFLFLKPSKNKPDGIKILMFLPLSKMKSFKIEME 429

BLAST of Carg15331 vs. ExPASy TrEMBL
Match: A0A6J1ECP4 (spermidine coumaroyl-CoA acyltransferase OS=Cucurbita moschata OX=3662 GN=LOC111433118 PE=3 SV=1)

HSP 1 Score: 932.2 bits (2408), Expect = 9.2e-268
Identity = 450/451 (99.78%), Postives = 450/451 (99.78%), Query Frame = 0

Query: 51  MRVTVSATTPILPSHPAFAHGHVLPLSHLDTDPNLRVSFRYLRAYVSTKAHQSPTPDPVD 110
           MRVTVSATTPILPSHPAFAHGHVLPLSHLDTDPNLRVSFRYLRAYVST AHQSPTPDPVD
Sbjct: 1   MRVTVSATTPILPSHPAFAHGHVLPLSHLDTDPNLRVSFRYLRAYVSTNAHQSPTPDPVD 60

Query: 111 VIATALSSALLHYYPLAGTLRRQNHRLELFCTHGQGVPLIRATADCELASVDYLDNPDET 170
           VIATALSSALLHYYPLAGTLRRQNHRLELFCTHGQGVPLIRATADCELASVDYLDNPDET
Sbjct: 61  VIATALSSALLHYYPLAGTLRRQNHRLELFCTHGQGVPLIRATADCELASVDYLDNPDET 120

Query: 171 FVEQLVPDPDENEGLNHPCILQVTLFECGGFTLGASIHHSVCDGLGATQFFNAVAELARG 230
           FVEQLVPDPDENEGLNHPCILQVTLFECGGFTLGASIHHSVCDGLGATQFFNAVAELARG
Sbjct: 121 FVEQLVPDPDENEGLNHPCILQVTLFECGGFTLGASIHHSVCDGLGATQFFNAVAELARG 180

Query: 231 ARTVSIEPVWDRASLLGPRNPPRIEVPIADFLSLEHGNLPYSQEVGAVVRECFPVTDDQL 290
           ARTVSIEPVWDRASLLGPRNPPRIEVPIADFLSLEHGNLPYSQEVGAVVRECFPVTDDQL
Sbjct: 181 ARTVSIEPVWDRASLLGPRNPPRIEVPIADFLSLEHGNLPYSQEVGAVVRECFPVTDDQL 240

Query: 291 ERFKSMLFKQSGSRFTAFEALGAYIWRAKVKASEIAGNEKVKFVYSTNIRKQLKPPLPVG 350
           ERFKSMLFKQSGSRFTAFEALGAYIWRAKVKASEIAGNEKVKFVYSTNIRKQLKPPLPVG
Sbjct: 241 ERFKSMLFKQSGSRFTAFEALGAYIWRAKVKASEIAGNEKVKFVYSTNIRKQLKPPLPVG 300

Query: 351 YYGNGCVPIYVTLTAEEIREQPIWETAKKIQKSKINISDEYARSFIDFQEIHCGEGITSG 410
           YYGNGCVPIYVTLTAEEIREQPIWETAKKIQKSKINISDEYARSFIDFQEIHCGEGITSG
Sbjct: 301 YYGNGCVPIYVTLTAEEIREQPIWETAKKIQKSKINISDEYARSFIDFQEIHCGEGITSG 360

Query: 411 KDVSAFTDWRHLGHSTVDFGWGGPATVLPLSRFLLGSVEPCFFLPHSSATSSTAGFKVSV 470
           KDVSAFTDWRHLGHSTVDFGWGGPATVLPLSRFLLGSVEPCFFLPHSSATSSTAGFKVSV
Sbjct: 361 KDVSAFTDWRHLGHSTVDFGWGGPATVLPLSRFLLGSVEPCFFLPHSSATSSTAGFKVSV 420

Query: 471 ALRKPAMPCFREEMKKFGDDDFGVIELNSLL 502
           ALRKPAMPCFREEMKKFGDDDFGVIELNSLL
Sbjct: 421 ALRKPAMPCFREEMKKFGDDDFGVIELNSLL 451

BLAST of Carg15331 vs. ExPASy TrEMBL
Match: A0A6J1IUR9 (spermidine coumaroyl-CoA acyltransferase OS=Cucurbita maxima OX=3661 GN=LOC111478580 PE=3 SV=1)

HSP 1 Score: 902.5 bits (2331), Expect = 7.8e-259
Identity = 434/451 (96.23%), Postives = 438/451 (97.12%), Query Frame = 0

Query: 51  MRVTVSATTPILPSHPAFAHGHVLPLSHLDTDPNLRVSFRYLRAYVSTKAHQSPTPDPVD 110
           MRVTVSATTPILPSHP FAH HVLPLSHLDTDPNLRVSFRYLRAYVST AHQSPTPDP D
Sbjct: 1   MRVTVSATTPILPSHPPFAHSHVLPLSHLDTDPNLRVSFRYLRAYVSTNAHQSPTPDPFD 60

Query: 111 VIATALSSALLHYYPLAGTLRRQNHRLELFCTHGQGVPLIRATADCELASVDYLDNPDET 170
           VIATALSSALLHYYPLAGTLR QNHRLELFC HGQGVPLIRATADCELASVDYLD PDET
Sbjct: 61  VIATALSSALLHYYPLAGTLRCQNHRLELFCAHGQGVPLIRATADCELASVDYLDKPDET 120

Query: 171 FVEQLVPDPDENEGLNHPCILQVTLFECGGFTLGASIHHSVCDGLGATQFFNAVAELARG 230
           FVEQLVPDPDENEGLNHPCILQVTLFECGGFTLGASIHHSVCDGLGATQFFNAVAELARG
Sbjct: 121 FVEQLVPDPDENEGLNHPCILQVTLFECGGFTLGASIHHSVCDGLGATQFFNAVAELARG 180

Query: 231 ARTVSIEPVWDRASLLGPRNPPRIEVPIADFLSLEHGNLPYSQEVGAVVRECFPVTDDQL 290
           ARTVSIEPVWDRASLLGPRNPPRIE+PIADFLSLEHGNLPYSQEVGAVVRECFPVTDDQL
Sbjct: 181 ARTVSIEPVWDRASLLGPRNPPRIELPIADFLSLEHGNLPYSQEVGAVVRECFPVTDDQL 240

Query: 291 ERFKSMLFKQSGSRFTAFEALGAYIWRAKVKASEIAGNEKVKFVYSTNIRKQLKPPLPVG 350
           ERFKSMLFKQSGSRFTAFEALGAYIWRAKVKASEIAGNEKVKFVYSTNIRKQLKPPLPVG
Sbjct: 241 ERFKSMLFKQSGSRFTAFEALGAYIWRAKVKASEIAGNEKVKFVYSTNIRKQLKPPLPVG 300

Query: 351 YYGNGCVPIYVTLTAEEIREQPIWETAKKIQKSKINISDEYARSFIDFQEIHCGEGITSG 410
           YYGNGCVPIYVTLTAEE+REQPIWETAKKIQKSKINISDEY RSFIDFQEIHCG+GIT G
Sbjct: 301 YYGNGCVPIYVTLTAEEVREQPIWETAKKIQKSKINISDEYVRSFIDFQEIHCGDGITPG 360

Query: 411 KDVSAFTDWRHLGHSTVDFGWGGPATVLPLSRFLLGSVEPCFFLPHSSATSSTAGFKVSV 470
           K+VSAFTDWRHLGHSTVDFGWGGP TVLPLSRFLLGSVEPCFFLPHSSAT S AGFKVSV
Sbjct: 361 KEVSAFTDWRHLGHSTVDFGWGGPVTVLPLSRFLLGSVEPCFFLPHSSATWSAAGFKVSV 420

Query: 471 ALRKPAMPCFREEMKKFGDDDFGVIELNSLL 502
           ALRKPAMPCFREEMKKFGDDDFGVIELN LL
Sbjct: 421 ALRKPAMPCFREEMKKFGDDDFGVIELNLLL 451

BLAST of Carg15331 vs. ExPASy TrEMBL
Match: A0A5D3CPT5 (Spermidine coumaroyl-CoA acyltransferase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold255G008000 PE=3 SV=1)

HSP 1 Score: 839.3 bits (2167), Expect = 8.2e-240
Identity = 402/454 (88.55%), Postives = 425/454 (93.61%), Query Frame = 0

Query: 46  SNSTAMRVTVSATTPILPSHPAFAHGHVLPLSHLDTDPNLRVSFRYLRAYVSTKAHQSPT 105
           SN T MRVTVSATT I PS PAFAH HVL LS LDTDPNLRV+FRYLRAYVST A QSP+
Sbjct: 114 SNKTTMRVTVSATTTIFPSRPAFAHNHVLSLSPLDTDPNLRVNFRYLRAYVSTNATQSPS 173

Query: 106 PDPVDVIATALSSALLHYYPLAGTLRRQNHRLELFCTHGQGVPLIRATADCELASVDYLD 165
           PDP DVIATALSSALLHYYPLAGTLRR +HRLE+FCTH QGVPLIRAT DCELASVDYLD
Sbjct: 174 PDPFDVIATALSSALLHYYPLAGTLRRHSHRLEVFCTHVQGVPLIRATVDCELASVDYLD 233

Query: 166 NPDETFVEQLVPDPDENEGLNHPCILQVTLFECGGFTLGASIHHSVCDGLGATQFFNAVA 225
           NPDETFVEQLVPDPDENEGLNHPCILQVTLFECGGFTLGASIHHSVCDGLGATQFFNAVA
Sbjct: 234 NPDETFVEQLVPDPDENEGLNHPCILQVTLFECGGFTLGASIHHSVCDGLGATQFFNAVA 293

Query: 226 ELARGARTVSIEPVWDRASLLGPRNPPRIEVPIADFLSLEHGNLPYSQEVGAVVRECFPV 285
           ELARGARTVSIEPVWDRA+LL PRN PRIE PI +FLSLEHG+LPYSQ+VGAVVRECFPV
Sbjct: 294 ELARGARTVSIEPVWDRANLLAPRNLPRIETPIGEFLSLEHGDLPYSQKVGAVVRECFPV 353

Query: 286 TDDQLERFKSMLFKQSGSRFTAFEALGAYIWRAKVKASEIAGNEKVKFVYSTNIRKQLKP 345
           TDDQLE+FKS+LFKQSGSRFTAFEALGAYIWRAKVKAS+IAGNEKVKFVYSTNIRKQLKP
Sbjct: 354 TDDQLEKFKSILFKQSGSRFTAFEALGAYIWRAKVKASKIAGNEKVKFVYSTNIRKQLKP 413

Query: 346 PLPVGYYGNGCVPIYVTLTAEEIREQPIWETAKKIQKSKINISDEYARSFIDFQEIHCGE 405
           PLPVGY+GNGCVPIYVT+TAEE+REQPIWETAKKIQKSKINI+DEY RSF+DFQEIH G+
Sbjct: 414 PLPVGYWGNGCVPIYVTVTAEELREQPIWETAKKIQKSKININDEYVRSFVDFQEIHRGD 473

Query: 406 GITSGKDVSAFTDWRHLGHSTVDFGWGGPATVLPLSRFLLGSVEPCFFLPHSSATSSTAG 465
           GIT+GK+VSAFTDWRHLGHSTVDFGWGGP  VLP+SRFLLGSVEPCFFLPHSSA +S AG
Sbjct: 474 GITAGKEVSAFTDWRHLGHSTVDFGWGGPVAVLPVSRFLLGSVEPCFFLPHSSAAASEAG 533

Query: 466 FKVSVALRKPAMPCFREEMKKFGDDDFGVIELNS 500
           FKVSV LRK AMP FREEMK+FGDD FGV+E NS
Sbjct: 534 FKVSVTLRKTAMPAFREEMKRFGDDHFGVVEQNS 567

BLAST of Carg15331 vs. ExPASy TrEMBL
Match: A0A5A7UBR6 (Spermidine coumaroyl-CoA acyltransferase OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold120G002480 PE=3 SV=1)

HSP 1 Score: 839.3 bits (2167), Expect = 8.2e-240
Identity = 402/454 (88.55%), Postives = 425/454 (93.61%), Query Frame = 0

Query: 46  SNSTAMRVTVSATTPILPSHPAFAHGHVLPLSHLDTDPNLRVSFRYLRAYVSTKAHQSPT 105
           SN T MRVTVSATT I PS PAFAH HVL LS LDTDPNLRV+FRYLRAYVST A QSP+
Sbjct: 94  SNKTTMRVTVSATTTIFPSRPAFAHNHVLSLSPLDTDPNLRVNFRYLRAYVSTNATQSPS 153

Query: 106 PDPVDVIATALSSALLHYYPLAGTLRRQNHRLELFCTHGQGVPLIRATADCELASVDYLD 165
           PDP DVIATALSSALLHYYPLAGTLRR +HRLE+FCTH QGVPLIRAT DCELASVDYLD
Sbjct: 154 PDPFDVIATALSSALLHYYPLAGTLRRHSHRLEVFCTHVQGVPLIRATVDCELASVDYLD 213

Query: 166 NPDETFVEQLVPDPDENEGLNHPCILQVTLFECGGFTLGASIHHSVCDGLGATQFFNAVA 225
           NPDETFVEQLVPDPDENEGLNHPCILQVTLFECGGFTLGASIHHSVCDGLGATQFFNAVA
Sbjct: 214 NPDETFVEQLVPDPDENEGLNHPCILQVTLFECGGFTLGASIHHSVCDGLGATQFFNAVA 273

Query: 226 ELARGARTVSIEPVWDRASLLGPRNPPRIEVPIADFLSLEHGNLPYSQEVGAVVRECFPV 285
           ELARGARTVSIEPVWDRA+LL PRN PRIE PI +FLSLEHG+LPYSQ+VGAVVRECFPV
Sbjct: 274 ELARGARTVSIEPVWDRANLLAPRNLPRIETPIGEFLSLEHGDLPYSQKVGAVVRECFPV 333

Query: 286 TDDQLERFKSMLFKQSGSRFTAFEALGAYIWRAKVKASEIAGNEKVKFVYSTNIRKQLKP 345
           TDDQLE+FKS+LFKQSGSRFTAFEALGAYIWRAKVKAS+IAGNEKVKFVYSTNIRKQLKP
Sbjct: 334 TDDQLEKFKSILFKQSGSRFTAFEALGAYIWRAKVKASKIAGNEKVKFVYSTNIRKQLKP 393

Query: 346 PLPVGYYGNGCVPIYVTLTAEEIREQPIWETAKKIQKSKINISDEYARSFIDFQEIHCGE 405
           PLPVGY+GNGCVPIYVT+TAEE+REQPIWETAKKIQKSKINI+DEY RSF+DFQEIH G+
Sbjct: 394 PLPVGYWGNGCVPIYVTVTAEELREQPIWETAKKIQKSKININDEYVRSFVDFQEIHRGD 453

Query: 406 GITSGKDVSAFTDWRHLGHSTVDFGWGGPATVLPLSRFLLGSVEPCFFLPHSSATSSTAG 465
           GIT+GK+VSAFTDWRHLGHSTVDFGWGGP  VLP+SRFLLGSVEPCFFLPHSSA +S AG
Sbjct: 454 GITAGKEVSAFTDWRHLGHSTVDFGWGGPVAVLPVSRFLLGSVEPCFFLPHSSAAASEAG 513

Query: 466 FKVSVALRKPAMPCFREEMKKFGDDDFGVIELNS 500
           FKVSV LRK AMP FREEMK+FGDD FGV+E NS
Sbjct: 514 FKVSVTLRKTAMPAFREEMKRFGDDHFGVVEQNS 547

BLAST of Carg15331 vs. ExPASy TrEMBL
Match: A0A1S3AZ43 (spermidine coumaroyl-CoA acyltransferase OS=Cucumis melo OX=3656 GN=LOC103484468 PE=3 SV=1)

HSP 1 Score: 837.8 bits (2163), Expect = 2.4e-239
Identity = 400/449 (89.09%), Postives = 423/449 (94.21%), Query Frame = 0

Query: 51  MRVTVSATTPILPSHPAFAHGHVLPLSHLDTDPNLRVSFRYLRAYVSTKAHQSPTPDPVD 110
           MRVTVSATTPI PS PAFAH HVL LS LDTDPNLRV+FRYLRAYVST A QSP+PDP D
Sbjct: 1   MRVTVSATTPIFPSRPAFAHNHVLSLSPLDTDPNLRVNFRYLRAYVSTNATQSPSPDPFD 60

Query: 111 VIATALSSALLHYYPLAGTLRRQNHRLELFCTHGQGVPLIRATADCELASVDYLDNPDET 170
           VIATALSSALLHYYPLAGT+RR +HRLE+FCTH QGVPLIRAT DCELASVDYLDNPDET
Sbjct: 61  VIATALSSALLHYYPLAGTIRRHSHRLEVFCTHVQGVPLIRATVDCELASVDYLDNPDET 120

Query: 171 FVEQLVPDPDENEGLNHPCILQVTLFECGGFTLGASIHHSVCDGLGATQFFNAVAELARG 230
           FVEQLVPDPDENEGLNHPCILQVTLFECGGFTLGASIHHSVCDGLGATQFFNAVAELARG
Sbjct: 121 FVEQLVPDPDENEGLNHPCILQVTLFECGGFTLGASIHHSVCDGLGATQFFNAVAELARG 180

Query: 231 ARTVSIEPVWDRASLLGPRNPPRIEVPIADFLSLEHGNLPYSQEVGAVVRECFPVTDDQL 290
           ARTVSIEPVWDRA+LL PRN PRIE PI +FLSLEHGNLPYSQ+VGAVVRECFPVTDDQL
Sbjct: 181 ARTVSIEPVWDRANLLAPRNLPRIETPIGEFLSLEHGNLPYSQKVGAVVRECFPVTDDQL 240

Query: 291 ERFKSMLFKQSGSRFTAFEALGAYIWRAKVKASEIAGNEKVKFVYSTNIRKQLKPPLPVG 350
           E+FKS+LFKQSGSRFTAFEALGAYIWRAKVKAS+IAGNEKVKFVYSTNIRKQLKPPLPVG
Sbjct: 241 EKFKSILFKQSGSRFTAFEALGAYIWRAKVKASKIAGNEKVKFVYSTNIRKQLKPPLPVG 300

Query: 351 YYGNGCVPIYVTLTAEEIREQPIWETAKKIQKSKINISDEYARSFIDFQEIHCGEGITSG 410
           Y+GNGCVPIYVT+TAEE+REQPIWETAKKIQKSKINI+DEY RSF+DFQEIH G+GIT+G
Sbjct: 301 YWGNGCVPIYVTVTAEELREQPIWETAKKIQKSKININDEYVRSFVDFQEIHRGDGITAG 360

Query: 411 KDVSAFTDWRHLGHSTVDFGWGGPATVLPLSRFLLGSVEPCFFLPHSSATSSTAGFKVSV 470
           K+VSAFTDWRHLGHSTVDFGWGGP  VLP+SRFLLGSVEPCFFLPHSSA +S AGFKVSV
Sbjct: 361 KEVSAFTDWRHLGHSTVDFGWGGPVAVLPVSRFLLGSVEPCFFLPHSSAAASEAGFKVSV 420

Query: 471 ALRKPAMPCFREEMKKFGDDDFGVIELNS 500
            LRK AMP FREEMK+FGDD FGV+E NS
Sbjct: 421 TLRKTAMPAFREEMKRFGDDHFGVVEQNS 449

BLAST of Carg15331 vs. TAIR 10
Match: AT1G28680.1 (HXXXD-type acyl-transferase family protein )

HSP 1 Score: 539.7 bits (1389), Expect = 2.6e-153
Identity = 261/443 (58.92%), Postives = 327/443 (73.81%), Query Frame = 0

Query: 61  ILPSHPAFAHGHVLPLSHLDTDPNLRVSFRYLRAY---VSTKAHQSPTPDPVDVIATALS 120
           + PSH   ++   L LSHLD D NL VSFRYLR Y    ST A +SP+     V++ +L+
Sbjct: 12  VQPSHQPLSNDQTLSLSHLDNDNNLHVSFRYLRVYSSSSSTVAGESPSA----VVSASLA 71

Query: 121 SALLHYYPLAGTLRR--QNHRLELFCTHGQGVPLIRATADCELASVDYLDNPDETFVEQL 180
           +AL+HYYPLAG+LRR   ++R EL C+ GQ VPL+ AT +C L SV YLD PD  FVE+L
Sbjct: 72  TALVHYYPLAGSLRRSASDNRFELLCSAGQSVPLVNATVNCTLESVGYLDGPDPGFVERL 131

Query: 181 VPDPDENEGLNHPCILQVTLFECGGFTLGASIHHSVCDGLGATQFFNAVAELARGARTVS 240
           VPDP   EG+ +PCILQVT+F+CGG+ LGASIHH++CDGLGA+ FFNA+AELARGA  +S
Sbjct: 132 VPDPTREEGMVNPCILQVTMFQCGGWVLGASIHHAICDGLGASLFFNAMAELARGATKIS 191

Query: 241 IEPVWDRASLLGPRNPPRIEVPIADFLSLEHGNLPYSQEVGAVVRECFPVTDDQLERFKS 300
           IEPVWDR  LLGPR  P +  P+ DFLSL+    PY Q +G V R+CF VTDD L++ K+
Sbjct: 192 IEPVWDRERLLGPREKPWVGAPVRDFLSLDKDFDPYGQAIGDVKRDCFFVTDDSLDQLKA 251

Query: 301 MLFKQSGSRFTAFEALGAYIWRAKVKASEIAGNEKVKFVYSTNIRKQLKPPLPVGYYGNG 360
            L ++SG  FT FEALGAYIWRAKV+A++    E VKFVYS NIR+ + PPLP GY+GNG
Sbjct: 252 QLLEKSGLNFTTFEALGAYIWRAKVRAAKTEEKENVKFVYSINIRRLMNPPLPKGYWGNG 311

Query: 361 CVPIYVTLTAEEIREQPIWETAKKIQKSKINISDEYARSFIDFQEIHCGEGITSGKDVSA 420
           CVP+Y  + A E+ EQPIW+TA+ I++SK N SDEY RSFIDFQE+H  +GI +G  V+ 
Sbjct: 312 CVPMYAQIKAGELIEQPIWKTAELIKQSKSNTSDEYVRSFIDFQELHHKDGINAGTGVTG 371

Query: 421 FTDWRHLGHSTVDFGWGGPATVLPLSRFLLGSVEPCFFLPHS----SATSSTAGFKVSVA 480
           FTDWR+LGHST+DFGWGGP TVLPLS  LLGS+EPCFFLP+S    + +   +GFKV V 
Sbjct: 372 FTDWRYLGHSTIDFGWGGPVTVLPLSNKLLGSMEPCFFLPYSTDAAAGSKKDSGFKVLVN 431

Query: 481 LRKPAMPCFREEMKKFGDDDFGV 495
           LR+ AMP F+E M KF   +F +
Sbjct: 432 LRESAMPEFKEAMDKFHKGEFAL 450

BLAST of Carg15331 vs. TAIR 10
Match: AT3G47170.1 (HXXXD-type acyl-transferase family protein )

HSP 1 Score: 228.8 bits (582), Expect = 9.7e-60
Identity = 157/473 (33.19%), Postives = 224/473 (47.36%), Query Frame = 0

Query: 44  RRSNSTAMRVTVSATTPILPSHPAFAHGHVLPLSHLDTDPNLRVSFRYLRAYVSTKAHQS 103
           R  NS    V  S    + PS P       L LS +D DPN  V    L  +      + 
Sbjct: 3   RSDNSCPFVVEKSEVVLVKPSKP--TPDVTLSLSTIDNDPNNEVILDILCVFSPNPYVRD 62

Query: 104 PTP-DPVDVIATALSSALLHYYPLAGTLRRQ--NHRLELFCTHGQGVPLIRATADCELAS 163
            T   P   I  ALS+AL++YYPLAG L R+  +  L+L CT G GVPLI+ATA C L+S
Sbjct: 63  HTNYHPASFIQLALSNALVYYYPLAGKLHRRTSDQTLQLDCTQGDGVPLIKATASCTLSS 122

Query: 164 VDYLDNPDE-TFVEQLVPDPDENEGLN---HPCILQVTLFECGGFTLGASIHHSVCDGLG 223
           ++YL++ +      QLVP  D  +G N    P  LQ+T F CGG T+G+   H+VCDG+G
Sbjct: 123 LNYLESGNHLEATYQLVPSHDTLKGCNLGYRPLALQITKFTCGGMTIGSVHSHTVCDGIG 182

Query: 224 ATQFFNAVAELARGARTVSIEPVWDR-----------ASLLGPRNPPRIEVPIADFLSLE 283
             QF  A+ ELA G    ++ PVWDR             L   +N P++       + +E
Sbjct: 183 MAQFSQAILELAAGRAQPTVIPVWDRHMITSNQISTLCKLGNDKNNPKL-------VDVE 242

Query: 284 HGNLPYSQEVGAVVRECFPVTDDQLERFKSMLF--------KQSGSRFTAFEALGAYIWR 343
                       +VRE   +T + + + K+++         K+   + T  E L AY+WR
Sbjct: 243 KDCSSPDTPTEDMVREILNITSEDITKLKNIIIEDENLTNEKEKNMKITTVEVLAAYVWR 302

Query: 344 AKVKASEIAGNEKVKFVYSTNIRKQLKPPLPVGYYGNGCVPIYVTLTAEEIREQPIWETA 403
           A+ +A ++  +     V S +IR  ++PPLP GYYGN      V LTA+E+ + PI    
Sbjct: 303 ARCRAMKLNPDTITDLVISVSIRSSIEPPLPEGYYGNAFTHASVALTAKELSKTPISRLV 362

Query: 404 KKIQKSKINISD-----EYARSFIDFQEIHCGEGITSGKDVSAFTDWRHLGHSTVDFGWG 463
           + I+ +K    D     E  R   +  ++        G      TDWRHLG       WG
Sbjct: 363 RLIKDAKRAALDNGYVCEQLREMENTMKLKLASKEIHGGVFMMLTDWRHLGLD--QDVWG 422

Query: 464 GPATVLPLSRFLLGSVEPCFFLPHSSAT-SSTAGFKVSVALRKPAMPCFREEM 485
           G   ++PL    L  +  C  LP S A    + G +V   L + AM  F+EE+
Sbjct: 423 GLVNIIPLVPLTLPFL--CVLLPASKAVPGKSGGVRVLTTLPRDAMAKFKEEI 462

BLAST of Carg15331 vs. TAIR 10
Match: AT2G25150.1 (HXXXD-type acyl-transferase family protein )

HSP 1 Score: 227.6 bits (579), Expect = 2.2e-59
Identity = 149/445 (33.48%), Postives = 223/445 (50.11%), Query Frame = 0

Query: 70  HGHVLPLSHLDTDPNLRVSFRYLRAYVSTKAHQSPTPDPVDVIATALSSALLHYYPLAGT 129
           H   L LS LD DP   V +  +  +   KA+     DPV ++  ALS  L+HYYPL+G 
Sbjct: 28  HCETLSLSTLDNDPFNEVMYATIYVF---KANGKNLDDPVSLLRKALSELLVHYYPLSGK 87

Query: 130 LRR--QNHRLELFCTHGQGVPLIRATADCELASVDYLDNPDETFVEQLVPDPD---ENEG 189
           L R   N +L+L    G+GVP   AT+  +L+S++Y++N D+    +LVP+ +   E+  
Sbjct: 88  LMRSESNGKLQLVYL-GEGVPFEVATSTLDLSSLNYIENLDDQVALRLVPEIEIDYESNV 147

Query: 190 LNHPCILQVTLFECGGFTLGASIHHSVCDGLGATQFFNAVAELARGARTVSIEPVWDRAS 249
             HP  LQVT F CGGFT+G ++ H+VCDG G  Q  +A+ ELA G    S++ VW R  
Sbjct: 148 CYHPLALQVTKFACGGFTIGTALTHAVCDGYGVAQIIHALTELAAGKTEPSVKSVWQRER 207

Query: 250 LLG--PRNPPRIEVPIADFLSLEHGNLPYSQEVGAVVRECFPVTDDQLERFKSMLFKQS- 309
           L+G     P ++     D        LP +     VV E   +    ++R K  + K+  
Sbjct: 208 LVGKIDNKPGKVPGSHIDGFLATSAYLPTTD----VVTETINIRAGDIKRLKDSMMKECE 267

Query: 310 --GSRFTAFEALGAYIWRAKVKASEIAGNEKVKFVYSTNIRKQLKPPLPVGYYGNGCVPI 369
                FT +E L +YIW+ + +A ++  +       +  IR  L PPLP GYYGN  + +
Sbjct: 268 YLKESFTTYEVLSSYIWKLRSRALKLNPDGITVLGVAVGIRHVLDPPLPKGYYGNAYIDV 327

Query: 370 YVTLTAEEIREQPIWETAKKIQKSKINISDEYARSFID---------FQEIHCGEGITSG 429
           YV LT  E+ E  I   A +++K+K      Y + +I+          ++    EG++ G
Sbjct: 328 YVELTVRELEESSISNIANRVKKAK---KTAYEKGYIEEELKNTERLMRDDSMFEGVSDG 387

Query: 430 KDVSAFTDWRHLG-HSTVDFGWGGPATVLPLSRFLLGSVEPCFFLPHSSATSSTAGFKVS 489
             +   TDWR++G   ++DFGW  P  + PL++            P  S  S   G KV 
Sbjct: 388 --LFFLTDWRNIGWFGSMDFGWNEPVNLRPLTQRESTVHVGMILKPSKSDPSMEGGVKVI 447

BLAST of Carg15331 vs. TAIR 10
Match: AT5G07080.1 (HXXXD-type acyl-transferase family protein )

HSP 1 Score: 226.1 bits (575), Expect = 6.3e-59
Identity = 151/427 (35.36%), Postives = 213/427 (49.88%), Query Frame = 0

Query: 74  LPLSHLDTDPNLRVSFRYLRAYVSTKAHQSPTPDPVDVIATALSSALLHYYPLAGTLRR- 133
           L LS LD DP L    + +  Y     +     DP  +   ALS AL++YYPLAG L R 
Sbjct: 33  LSLSTLDNDPYLETLAKTIYVYAPPSPNDH-VHDPASLFQQALSDALVYYYPLAGKLHRG 92

Query: 134 -QNHRLELFCTHGQGVPLIRATADCELASVDYLDNPDETFVEQLVP-DPDENEGLNHPCI 193
             +HRLEL C+  +GVP +RATADC L+S++YL + D T + QLVP D     G  +P  
Sbjct: 93  SHDHRLELRCSPAEGVPFVRATADCTLSSLNYLKDMD-TDLYQLVPCDVAAPSGDYNPLA 152

Query: 194 LQVTLFECGGFTLGASIHHSVCDGLGATQFFNAVAELARGARTVSIEPVWDRASLLGPRN 253
           LQ+TLF CGG TL  ++ HS+CDG GA+QFF  + ELA G    SI PVWDR  L     
Sbjct: 153 LQITLFACGGLTLATALSHSLCDGFGASQFFKTLTELAAGKTQPSIIPVWDRHRLTSNNF 212

Query: 254 PPRIEV------PIADF--LSLEHGNLPYSQEVGAVVRECFPVTDDQLERFKSMLFKQSG 313
               +V       + DF          PY+     +V E   VT + + + K    ++  
Sbjct: 213 SLNDQVEEGQAPKLVDFGEACSSAATSPYTPS-NDMVCEILNVTSEDITQLK----EKVA 272

Query: 314 SRFTAFEALGAYIWRAKVKASEIAGNEKVKFVYSTNIRKQLKPPLPVGYYGNGCVPIYVT 373
              T  E L A++WRA+ +A +++ +    F  +  IR+ ++PPLP GYYGN  V   V 
Sbjct: 273 GVVTTLEILAAHVWRARCRALKLSPDGTSLFGMAVGIRRIVEPPLPEGYYGNAFVKANVA 332

Query: 374 LTAEEIREQPIWETAKKIQKSKINISDE-YARSFIDFQEIHCGEGITSGKDVSAF---TD 433
           + A E+   P+    + I+++K    ++ Y    +   E      +       AF   TD
Sbjct: 333 MKAGELSNSPLSHVVQLIKEAKKAAQEKRYVLEQLRETEKTLKMNVACEGGKGAFMLLTD 392

Query: 434 WRHLG-HSTVDFGWGGPATVLPLSRFLLGSVEPCFFLPHSSATSSTAGFKVSVALRKPAM 485
           WR LG    +DFG+GG   ++PL    L  +  C FLP         G +V V L K  M
Sbjct: 393 WRQLGLLDEIDFGYGGSVNIIPLVPKYLPDI--CIFLPRKQ-----GGVRVLVTLPKSVM 445

BLAST of Carg15331 vs. TAIR 10
Match: AT2G23510.1 (spermidine disinapoyl acyltransferase )

HSP 1 Score: 208.8 bits (530), Expect = 1.0e-53
Identity = 144/426 (33.80%), Postives = 211/426 (49.53%), Query Frame = 0

Query: 74  LPLSHLDTDPNLRVSFRYLRAYVSTKAHQSPTPD--PVDVIATALSSALLHYYPLAGTLR 133
           L LS LD DP   V ++    +   KA      D  P  ++  ALS  L +YYPL+G+L+
Sbjct: 32  LNLSTLDNDPYNEVIYKACYVF---KAKNVADDDNRPEALLREALSDLLGYYYPLSGSLK 91

Query: 134 RQ--NHRLELFC-THGQGVPLIRATADCELASVDYLDNPDETFVEQLVPDPDENEGLNHP 193
           RQ  + +L+L C   G GVP   ATA+ EL+S+  L+N D       +P    +     P
Sbjct: 92  RQESDRKLQLSCGGDGGGVPFTVATANVELSSLKNLENIDSDTALNFLPVLHVDIDGYRP 151

Query: 194 CILQVTLFECGGFTLGASIHHSVCDGLGATQFFNAVAELARGARTVSIEPVWDRASLLG- 253
             LQVT FECGGF LG ++ H++CDG G      A+ +LA G +   + P+W+R  L+G 
Sbjct: 152 FALQVTKFECGGFILGMAMSHAMCDGYGEGHIMCALTDLAGGKKKPMVTPIWERERLVGK 211

Query: 254 PRNPPRIEVPIADFLSLEHGNLPYSQEVGAVVRECFPVTDDQLERFKSMLFKQ---SGSR 313
           P +     VP  D  +  +  LP        V E   +  D + R K    K+   S   
Sbjct: 212 PEDDQPPFVPGDDTAASPY--LPTDD----WVTEKITIRADSIRRLKEATLKEYDFSNET 271

Query: 314 FTAFEALGAYIWRAKVKASEIAGNEKVKFVYSTNIRKQLKPPLPVGYYGNGCVPIYVTLT 373
            T FE +GAY+W+++VKA  +  +       S  IR  + PPLP GYYGN  + +YV LT
Sbjct: 272 ITTFEVIGAYLWKSRVKALNLDRDGVTVLGLSVGIRNVVDPPLPDGYYGNAYIDMYVPLT 331

Query: 374 AEEIREQPIWETAKKIQKSKINISD-EYARSFIDFQE--IHCGEGITSGKD-VSAFTDWR 433
           A E+ E  I +  K I+++K N  D +Y +  +   E  I     I   KD +   TDWR
Sbjct: 332 AREVEEFTISDIVKLIKEAKRNAHDKDYLQEELANTEKIIKMNLTIKGKKDGLFCLTDWR 391

Query: 434 HLG-HSTVDFGWGGPATVLPLSRFLLGSVEPCFFLPHSSATSSTAGFKVSVALRKPAMPC 486
           ++G   ++DFGW  P  ++P+           F  P    +    G ++ V L + AM  
Sbjct: 392 NIGIFGSMDFGWDEPVNIVPVVPSETARTVNMFMRPSRLESDMVGGVQIVVTLPRIAMVK 448

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
KAG7034631.12.5e-299100.00Spermidine coumaroyl-CoA acyltransferase, partial [Cucurbita argyrosperma subsp.... [more]
KAG6581346.13.9e-29799.20Spermidine coumaroyl-CoA acyltransferase, partial [Cucurbita argyrosperma subsp.... [more]
XP_022925827.11.9e-26799.78spermidine coumaroyl-CoA acyltransferase [Cucurbita moschata][more]
XP_023543910.11.4e-26297.34spermidine coumaroyl-CoA acyltransferase [Cucurbita pepo subsp. pepo][more]
XP_022978699.11.6e-25896.23spermidine coumaroyl-CoA acyltransferase [Cucurbita maxima][more]
Match NameE-valueIdentityDescription
Q8GYW83.0e-5833.48Spermidine coumaroyl-CoA acyltransferase OS=Arabidopsis thaliana OX=3702 GN=SCT ... [more]
O804671.5e-5233.80Spermidine sinapoyl-CoA acyltransferase OS=Arabidopsis thaliana OX=3702 GN=SDT P... [more]
Q8LL695.6e-5233.333'-N-debenzoyl-2'-deoxytaxol N-benzoyltransferase OS=Taxus canadensis OX=88032 G... [more]
Q8S9G64.0e-5030.39Taxadien-5-alpha-ol O-acetyltransferase OS=Taxus wallichiana var. chinensis OX=2... [more]
Q9M6F01.2e-4931.25Taxadien-5-alpha-ol O-acetyltransferase OS=Taxus cuspidata OX=99806 GN=TAT PE=1 ... [more]
Match NameE-valueIdentityDescription
A0A6J1ECP49.2e-26899.78spermidine coumaroyl-CoA acyltransferase OS=Cucurbita moschata OX=3662 GN=LOC111... [more]
A0A6J1IUR97.8e-25996.23spermidine coumaroyl-CoA acyltransferase OS=Cucurbita maxima OX=3661 GN=LOC11147... [more]
A0A5D3CPT58.2e-24088.55Spermidine coumaroyl-CoA acyltransferase OS=Cucumis melo var. makuwa OX=1194695 ... [more]
A0A5A7UBR68.2e-24088.55Spermidine coumaroyl-CoA acyltransferase OS=Cucumis melo var. makuwa OX=1194695 ... [more]
A0A1S3AZ432.4e-23989.09spermidine coumaroyl-CoA acyltransferase OS=Cucumis melo OX=3656 GN=LOC103484468... [more]
Match NameE-valueIdentityDescription
AT1G28680.12.6e-15358.92HXXXD-type acyl-transferase family protein [more]
AT3G47170.19.7e-6033.19HXXXD-type acyl-transferase family protein [more]
AT2G25150.12.2e-5933.48HXXXD-type acyl-transferase family protein [more]
AT5G07080.16.3e-5935.36HXXXD-type acyl-transferase family protein [more]
AT2G23510.11.0e-5333.80spermidine disinapoyl acyltransferase [more]
InterPro
Analysis Name: InterPro Annotations of Silver-seed gourd (SMH-JMG-627) v2
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR023213Chloramphenicol acetyltransferase-like domain superfamilyGENE3D3.30.559.10coord: 47..262
e-value: 5.6E-50
score: 171.7
IPR023213Chloramphenicol acetyltransferase-like domain superfamilyGENE3D3.30.559.10coord: 271..484
e-value: 1.1E-40
score: 141.1
IPR003480TransferasePFAMPF02458Transferasecoord: 51..485
e-value: 6.8E-73
score: 245.8
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 1..32
NoneNo IPR availablePANTHERPTHR31147:SF33N-HYDROXYCINNAMOYL/BENZOYLTRANSFERASE, PUTATIVE-RELATEDcoord: 51..489
NoneNo IPR availablePANTHERPTHR31147ACYL TRANSFERASE 4coord: 51..489

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Carg15331-RACarg15331-RAmRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0009805 coumarin biosynthetic process
molecular_function GO:0016410 N-acyltransferase activity
molecular_function GO:0016747 acyltransferase activity, transferring groups other than amino-acyl groups