Carg15327 (gene) Silver-seed gourd (SMH-JMG-627) v2

Overview


Name	Carg15327
Type	gene
Organism	Cucurbita argyrosperma subsp. argyrosperma cv. SMH-JMG-627 (Silver-seed gourd (SMH-JMG-627) v2)
Description	ATP synthase mitochondrial F1 complex assembly factor 1
Location	Carg_Chr03: 9301765 .. 9304159 (+)
RNA-Seq Expression	Carg15327
Synteny	Carg15327

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRCDSpolypeptidethree_prime_UTR

Hold the cursor over a type above to highlight its positions in the sequence below.

TGTGATAAAAGTATTGTTTACCCGATTGATTTTTTAATTTCATTTCTCCGCGATGCGAAGCTCTTCGTTACGACCGTTGAGGACCATTGCTGGTTCTGCGAAATTTTTTCTCGCTAGTGCTTCGCCGGCGGTTCGACAATCTTCTCGTTCCTATTTTGCTAATGCGAGTCTTGAGAAACTGCAGAAGGAAGCTATTCCTTGTGATTTCCTCAAATGGTGTTCGATTGGTTTCTTTCGGACTTCAAGATTCGCTTCGGGGTTCGCCCCCTTGCAACAAAAGCCGTTGGATTCGATTATGGATATGGAGAGGGCGAAAGATCGGTCACCGGAGGACCTCGCTTCCATCTGGGATGATGTAAGGTTTCTTCTTCTTCTTGTTAATTTTGTTTAATTTTAGTCACAATATTCGTTTCTGTTTGTAAGATTCCAAAATTCTTTGTAGTCGAATTGTGTAATCTCTAGGATCGGTTAATTTGGTAGAAGAGATTAATGTGCGAGAAAAGACTTGGAGTATTGTTAGAACTTAAAATCGGCGGAACCTTTGTAGTTTGTAGTCATGAATGAGAGAGCTCCATTTTGAATCTAGTCACTTGCTCCTCCGAATTCCAGATGAAATGAATTTTATACTACACACATCCAAAGTATGTGTGTAGTATAAAATTCATTTATTGGATGGATAATCTAACCTTTGTAGTTTCCAGAATCTAGAACCGAGAAGCGAAAGTGCCCCCCTTTGGATGGATAATCCATTTATTGGGACAACGACAATGGCTTCGTAAATTGTTCTTCAATGATTGTATACATTTTTTCATTTAGAGGTTCTTCAATGGAAGAAATTTTTTAGCTAACTCAAACCTTGGTTAAATGTTATCAGTGTTAGTAACTTGAGTGAAACCATGGATGATTTTGTTGTTGTCACTTTTTCCCCGATATGTGCAATTATTCTCACATTCTCTTGCCGGGTTCTGCGCTACCAGTATCATTTGGGAAGAGGTCATATTGGGGCATCCATGAAAGCTAAACTATATCATTTATTGGAACAACGAGCAGCAGATTGGTAATTATCAAACATTTTATAATTCCCGGTTTGATTCATTTTCAGTATTTTGCCGTTCTAAGTTTAGTGCAATCTTGCAGCCGACACTTCGTCATTCCTTTGTGGCGTGGAAGTGGTTATACTACCATGTTTGTTCAAGGTTTGATCTTTAACTCGTGGCTTGAAGTTATATGTTTATATTAATTGAAAGAAAGGGTCCTTATCTGAAAGTTATATAATTTGATGCCGTTACTTAATCCCTTCATGTGTTTTCTTTTTCTCCCCCTCTCTTTGTTTAATAGAAATAGGAATAATATTTGAAGAAAAAATAAATACATAGAAATTAGAATACCATCAAGGGATTAATCAAAGGGTTATTCTACAGTCATCCATCGGTTTATTTTTTCTTCAAATAAACCTGATATATTTGAAACACCAAAGCTTGATGGTAAAGACCAAGCCTTGAAGAAACATGTGAATTACCATCAAGCTTTGGTGTTTCAAAGAAATCAGGTTTCTTCTACAATAATGAACTTCAGGTTGCTATGGGAGAGGGTCTTAAATCTGGATGAAACGATTTCGTGGTCTTATCTTTGCTTGCTGTAATTGTTTATAGTTTAGAAGTTTGAAGTCTTGCTTAATCAAAACAGTGCTGGAAAATTGTTGGTGAACGAGATCTTATGTATATTTGGGGTGTGGGATATAAAACATAGAATTTAGTCTTGCCAGCAATGCTTATAGTTCAAGTTAAATGCTTAACTCGGTTATATATGCAAATCATTTTGCAGTTCAAATGCCGTATATCCTTTTCACTGGCCTTGAAGATTACAAGGCAAGAGGAACTCAAGCAGCTCCCTACTTCACCGTGGCCTATTACAAAGAATTTTCAGAGAGCAAGGACTTAGTGCTTATTCGAGGTGATATTGTGTTTACAAGTAAGCTCACCGACCAGGAGGCAGAATGGCTTTTAGAGACCACACAATCCTTCTATCTGAACGATGCTCGGTACAAGCTGGTTGAACGGTTTAACAAACAAACACATGAATTTGAGTTCAAGGATGTCTTACAAGCACTGGAAATGCCCATTTTATGATTTCTGCGGAGCTTAGCAGGGAATAGTGCCTTCTTGAAGGTAATAATAAGAAATTTAGGAAAACATGACCAGCCTTTGTAAGGCTATCATTTATAGTTCCTCATAACTTTGCATAGGCTGTTTTGACCTTTAGAAAGAACCATCCTAGTTCATAAATTTGAGGACAACGTCATAAATTGAATCCCAAAATTTAATTTGTCTGATTGGGTGAAAATTGGATTATGATATCCATTTTTTCTTTTAAATTTAATTTATAATTTTTGAGT

mRNA sequence

TGTGATAAAAGTATTGTTTACCCGATTGATTTTTTAATTTCATTTCTCCGCGATGCGAAGCTCTTCGTTACGACCGTTGAGGACCATTGCTGGTTCTGCGAAATTTTTTCTCGCTAGTGCTTCGCCGGCGGTTCGACAATCTTCTCGTTCCTATTTTGCTAATGCGAGTCTTGAGAAACTGCAGAAGGAAGCTATTCCTTGTGATTTCCTCAAATGGTGTTCGATTGGTTTCTTTCGGACTTCAAGATTCGCTTCGGGGTTCGCCCCCTTGCAACAAAAGCCGTTGGATTCGATTATGGATATGGAGAGGGCGAAAGATCGGTCACCGGAGGACCTCGCTTCCATCTGGGATGATTATCATTTGGGAAGAGGTCATATTGGGGCATCCATGAAAGCTAAACTATATCATTTATTGGAACAACGAGCAGCAGATTGCCGACACTTCGTCATTCCTTTGTGGCGTGGAAGTGGTTATACTACCATGTTTGTTCAAGTTCAAATGCCGTATATCCTTTTCACTGGCCTTGAAGATTACAAGGCAAGAGGAACTCAAGCAGCTCCCTACTTCACCGTGGCCTATTACAAAGAATTTTCAGAGAGCAAGGACTTAGTGCTTATTCGAGGTGATATTGTGTTTACAAGTAAGCTCACCGACCAGGAGGCAGAATGGCTTTTAGAGACCACACAATCCTTCTATCTGAACGATGCTCGGTACAAGCTGGTTGAACGGTTTAACAAACAAACACATGAATTTGAGTTCAAGGATGTCTTACAAGCACTGGAAATGCCCATTTTATGATTTCTGCGGAGCTTAGCAGGGAATAGTGCCTTCTTGAAGGTAATAATAAGAAATTTAGGAAAACATGACCAGCCTTTGTAAGGCTATCATTTATAGTTCCTCATAACTTTGCATAGGCTGTTTTGACCTTTAGAAAGAACCATCCTAGTTCATAAATTTGAGGACAACGTCATAAATTGAATCCCAAAATTTAATTTGTCTGATTGGGTGAAAATTGGATTATGATATCCATTTTTTCTTTTAAATTTAATTTATAATTTTTGAGT

Coding sequence (CDS)

ATGCGAAGCTCTTCGTTACGACCGTTGAGGACCATTGCTGGTTCTGCGAAATTTTTTCTCGCTAGTGCTTCGCCGGCGGTTCGACAATCTTCTCGTTCCTATTTTGCTAATGCGAGTCTTGAGAAACTGCAGAAGGAAGCTATTCCTTGTGATTTCCTCAAATGGTGTTCGATTGGTTTCTTTCGGACTTCAAGATTCGCTTCGGGGTTCGCCCCCTTGCAACAAAAGCCGTTGGATTCGATTATGGATATGGAGAGGGCGAAAGATCGGTCACCGGAGGACCTCGCTTCCATCTGGGATGATTATCATTTGGGAAGAGGTCATATTGGGGCATCCATGAAAGCTAAACTATATCATTTATTGGAACAACGAGCAGCAGATTGCCGACACTTCGTCATTCCTTTGTGGCGTGGAAGTGGTTATACTACCATGTTTGTTCAAGTTCAAATGCCGTATATCCTTTTCACTGGCCTTGAAGATTACAAGGCAAGAGGAACTCAAGCAGCTCCCTACTTCACCGTGGCCTATTACAAAGAATTTTCAGAGAGCAAGGACTTAGTGCTTATTCGAGGTGATATTGTGTTTACAAGTAAGCTCACCGACCAGGAGGCAGAATGGCTTTTAGAGACCACACAATCCTTCTATCTGAACGATGCTCGGTACAAGCTGGTTGAACGGTTTAACAAACAAACACATGAATTTGAGTTCAAGGATGTCTTACAAGCACTGGAAATGCCCATTTTATGA

Protein sequence

MRSSSLRPLRTIAGSAKFFLASASPAVRQSSRSYFANASLEKLQKEAIPCDFLKWCSIGFFRTSRFASGFAPLQQKPLDSIMDMERAKDRSPEDLASIWDDYHLGRGHIGASMKAKLYHLLEQRAADCRHFVIPLWRGSGYTTMFVQVQMPYILFTGLEDYKARGTQAAPYFTVAYYKEFSESKDLVLIRGDIVFTSKLTDQEAEWLLETTQSFYLNDARYKLVERFNKQTHEFEFKDVLQALEMPIL

Homology

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BLAST of Carg15327 vs. NCBI nr
Match: KAG7034627.1 (ATP synthase mitochondrial F1 complex assembly factor 1 [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 499.6 bits (1285), Expect = 1.6e-137
Identity = 248/248 (100.00%), Postives = 248/248 (100.00%), Query Frame = 0


Query: 1   MRSSSLRPLRTIAGSAKFFLASASPAVRQSSRSYFANASLEKLQKEAIPCDFLKWCSIGF 60
           MRSSSLRPLRTIAGSAKFFLASASPAVRQSSRSYFANASLEKLQKEAIPCDFLKWCSIGF
Sbjct: 1   MRSSSLRPLRTIAGSAKFFLASASPAVRQSSRSYFANASLEKLQKEAIPCDFLKWCSIGF 60

Query: 61  FRTSRFASGFAPLQQKPLDSIMDMERAKDRSPEDLASIWDDYHLGRGHIGASMKAKLYHL 120
           FRTSRFASGFAPLQQKPLDSIMDMERAKDRSPEDLASIWDDYHLGRGHIGASMKAKLYHL
Sbjct: 61  FRTSRFASGFAPLQQKPLDSIMDMERAKDRSPEDLASIWDDYHLGRGHIGASMKAKLYHL 120

Query: 121 LEQRAADCRHFVIPLWRGSGYTTMFVQVQMPYILFTGLEDYKARGTQAAPYFTVAYYKEF 180
           LEQRAADCRHFVIPLWRGSGYTTMFVQVQMPYILFTGLEDYKARGTQAAPYFTVAYYKEF
Sbjct: 121 LEQRAADCRHFVIPLWRGSGYTTMFVQVQMPYILFTGLEDYKARGTQAAPYFTVAYYKEF 180

Query: 181 SESKDLVLIRGDIVFTSKLTDQEAEWLLETTQSFYLNDARYKLVERFNKQTHEFEFKDVL 240
           SESKDLVLIRGDIVFTSKLTDQEAEWLLETTQSFYLNDARYKLVERFNKQTHEFEFKDVL
Sbjct: 181 SESKDLVLIRGDIVFTSKLTDQEAEWLLETTQSFYLNDARYKLVERFNKQTHEFEFKDVL 240

Query: 241 QALEMPIL 249
           QALEMPIL
Sbjct: 241 QALEMPIL 248

[Close]

BLAST of Carg15327 vs. NCBI nr
Match: XP_022925840.1 (ATP synthase mitochondrial F1 complex assembly factor 1 [Cucurbita moschata])

HSP 1 Score: 492.7 bits (1267), Expect = 1.9e-135
Identity = 244/248 (98.39%), Postives = 246/248 (99.19%), Query Frame = 0


Query: 1   MRSSSLRPLRTIAGSAKFFLASASPAVRQSSRSYFANASLEKLQKEAIPCDFLKWCSIGF 60
           MRSSSLR LRTIAGSAKFFLASASPAVRQSSRSYFAN SLEKLQKEAIPCDFLKWCSIGF
Sbjct: 1   MRSSSLRTLRTIAGSAKFFLASASPAVRQSSRSYFANVSLEKLQKEAIPCDFLKWCSIGF 60

Query: 61  FRTSRFASGFAPLQQKPLDSIMDMERAKDRSPEDLASIWDDYHLGRGHIGASMKAKLYHL 120
           FRTSRFASGFAPLQQKPLDSIMDMERAKDRSPEDLASIWDDYHLGRGHIGASMKAKLYHL
Sbjct: 61  FRTSRFASGFAPLQQKPLDSIMDMERAKDRSPEDLASIWDDYHLGRGHIGASMKAKLYHL 120

Query: 121 LEQRAADCRHFVIPLWRGSGYTTMFVQVQMPYILFTGLEDYKARGTQAAPYFTVAYYKEF 180
           LEQRAADCRHFVIPLWRGSGYTTMFVQVQMPYILFTGLEDYKARGTQAAPYFTVAYYKEF
Sbjct: 121 LEQRAADCRHFVIPLWRGSGYTTMFVQVQMPYILFTGLEDYKARGTQAAPYFTVAYYKEF 180

Query: 181 SESKDLVLIRGDIVFTSKLTDQEAEWLLETTQSFYLNDARYKLVERFNKQTHEFEFKDVL 240
           +ESKDLVLIRGDIVFTSKLTD+EAEWLLETTQSFYLNDARYKLVERFNKQTHEFEFKDVL
Sbjct: 181 AESKDLVLIRGDIVFTSKLTDEEAEWLLETTQSFYLNDARYKLVERFNKQTHEFEFKDVL 240

Query: 241 QALEMPIL 249
           QALEMPIL
Sbjct: 241 QALEMPIL 248

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BLAST of Carg15327 vs. NCBI nr
Match: XP_023544293.1 (ATP synthase mitochondrial F1 complex assembly factor 1 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 485.7 bits (1249), Expect = 2.3e-133
Identity = 241/248 (97.18%), Postives = 243/248 (97.98%), Query Frame = 0


Query: 1   MRSSSLRPLRTIAGSAKFFLASASPAVRQSSRSYFANASLEKLQKEAIPCDFLKWCSIGF 60
           MRSSSLR LRTIAGSAKFFLASA PAVRQSSRSYF N SLEKLQKEAIPCDFLKWCSIGF
Sbjct: 1   MRSSSLRTLRTIAGSAKFFLASALPAVRQSSRSYFVNVSLEKLQKEAIPCDFLKWCSIGF 60

Query: 61  FRTSRFASGFAPLQQKPLDSIMDMERAKDRSPEDLASIWDDYHLGRGHIGASMKAKLYHL 120
           FRTSRFASGFAPLQQKPLDSIMDME+AKDRSPEDLASIWDDYHLGRGHIGASMKAKLYHL
Sbjct: 61  FRTSRFASGFAPLQQKPLDSIMDMEKAKDRSPEDLASIWDDYHLGRGHIGASMKAKLYHL 120

Query: 121 LEQRAADCRHFVIPLWRGSGYTTMFVQVQMPYILFTGLEDYKARGTQAAPYFTVAYYKEF 180
           LEQRAADCRHFVIPLWRGSGYTTMFVQVQMPYILFTGLEDYKARGTQAAPYFTVAYYKEF
Sbjct: 121 LEQRAADCRHFVIPLWRGSGYTTMFVQVQMPYILFTGLEDYKARGTQAAPYFTVAYYKEF 180

Query: 181 SESKDLVLIRGDIVFTSKLTDQEAEWLLETTQSFYLNDARYKLVERFNKQTHEFEFKDVL 240
           SESKDLVLIRGDIVFTSKLTDQEAEWLLETTQSFYLNDARYKLVERFNKQT +FEFKDVL
Sbjct: 181 SESKDLVLIRGDIVFTSKLTDQEAEWLLETTQSFYLNDARYKLVERFNKQTRDFEFKDVL 240

Query: 241 QALEMPIL 249
           QALEMPIL
Sbjct: 241 QALEMPIL 248

[Close]

BLAST of Carg15327 vs. NCBI nr
Match: XP_022978750.1 (ATP synthase mitochondrial F1 complex assembly factor 1 [Cucurbita maxima])

HSP 1 Score: 481.1 bits (1237), Expect = 5.8e-132
Identity = 239/248 (96.37%), Postives = 243/248 (97.98%), Query Frame = 0


Query: 1   MRSSSLRPLRTIAGSAKFFLASASPAVRQSSRSYFANASLEKLQKEAIPCDFLKWCSIGF 60
           MRSSSLR LRTIAGSAK FLASASPAVRQSSRSYFAN SLEKLQKEAIPCDFLKWCSIGF
Sbjct: 1   MRSSSLRTLRTIAGSAKNFLASASPAVRQSSRSYFANVSLEKLQKEAIPCDFLKWCSIGF 60

Query: 61  FRTSRFASGFAPLQQKPLDSIMDMERAKDRSPEDLASIWDDYHLGRGHIGASMKAKLYHL 120
           FRTSRFASGFAPLQQKPLDSIMDMERAKDRSPEDLASIWDDYHLGRGHIGASMKAKLYHL
Sbjct: 61  FRTSRFASGFAPLQQKPLDSIMDMERAKDRSPEDLASIWDDYHLGRGHIGASMKAKLYHL 120

Query: 121 LEQRAADCRHFVIPLWRGSGYTTMFVQVQMPYILFTGLEDYKARGTQAAPYFTVAYYKEF 180
           LEQRA+DCRHFVIPLWRGSGYTTMFVQVQMPYIL TGLEDYKARGTQAAPYFTVAYYKEF
Sbjct: 121 LEQRASDCRHFVIPLWRGSGYTTMFVQVQMPYILLTGLEDYKARGTQAAPYFTVAYYKEF 180

Query: 181 SESKDLVLIRGDIVFTSKLTDQEAEWLLETTQSFYLNDARYKLVERFNKQTHEFEFKDVL 240
           +ESKDLVLIRGDIVFTSKLTDQEAEWLLETTQSFYLNDARYKLVE+FNKQT +FEFKDVL
Sbjct: 181 AESKDLVLIRGDIVFTSKLTDQEAEWLLETTQSFYLNDARYKLVEQFNKQTRDFEFKDVL 240

Query: 241 QALEMPIL 249
           QALEMPIL
Sbjct: 241 QALEMPIL 248

[Close]

BLAST of Carg15327 vs. NCBI nr
Match: XP_038883536.1 (ATP synthase mitochondrial F1 complex assembly factor 1 [Benincasa hispida])

HSP 1 Score: 456.1 bits (1172), Expect = 2.0e-124
Identity = 223/248 (89.92%), Postives = 235/248 (94.76%), Query Frame = 0


Query: 1   MRSSSLRPLRTIAGSAKFFLASASPAVRQSSRSYFANASLEKLQKEAIPCDFLKWCSIGF 60
           MRSSSLR  RTIAGS K  LASASP VRQSSRSYFAN  LEKLQKEAIPCDFLKW S+GF
Sbjct: 1   MRSSSLRTSRTIAGSVKTLLASASPVVRQSSRSYFANVRLEKLQKEAIPCDFLKWSSLGF 60

Query: 61  FRTSRFASGFAPLQQKPLDSIMDMERAKDRSPEDLASIWDDYHLGRGHIGASMKAKLYHL 120
           FRTSRFA+GFAPLQ KPLDSI+DMERAKDRSPEDLASIWDDYHLGRGHIG SMKAKLYHL
Sbjct: 61  FRTSRFATGFAPLQPKPLDSIIDMERAKDRSPEDLASIWDDYHLGRGHIGVSMKAKLYHL 120

Query: 121 LEQRAADCRHFVIPLWRGSGYTTMFVQVQMPYILFTGLEDYKARGTQAAPYFTVAYYKEF 180
           LEQRAADCR+FVIP+WRGSGYTTMFVQVQ+PYILFTGLEDYKARGTQAAPYFTV+YYKEF
Sbjct: 121 LEQRAADCRYFVIPMWRGSGYTTMFVQVQLPYILFTGLEDYKARGTQAAPYFTVSYYKEF 180

Query: 181 SESKDLVLIRGDIVFTSKLTDQEAEWLLETTQSFYLNDARYKLVERFNKQTHEFEFKDVL 240
           +ESKDLVL+RGDIVFTSKLTDQEAEWLLETTQSFYLND RYKL+ERFN+QT +FEFKDVL
Sbjct: 181 AESKDLVLVRGDIVFTSKLTDQEAEWLLETTQSFYLNDVRYKLLERFNRQTRDFEFKDVL 240

Query: 241 QALEMPIL 249
           QALEMPIL
Sbjct: 241 QALEMPIL 248

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BLAST of Carg15327 vs. ExPASy Swiss-Prot
Match: Q66JD1 (ATP synthase mitochondrial F1 complex assembly factor 1 OS=Xenopus tropicalis OX=8364 GN=atpaf1 PE=2 SV=1)

HSP 1 Score: 87.0 bits (214), Expect = 3.2e-16
Identity = 57/180 (31.67%), Postives = 91/180 (50.56%), Query Frame = 0


Query: 67  ASGFAPLQQKPLDSIMDMERAKDRSPEDLASIWDDYHLGRGHIGASMKAKLYHLLEQRAA 126
           ASG    + K LDSI+++E  KD+  +++  IW  Y   R  + A +  + + L+ +RA 
Sbjct: 104 ASGKGFSKNKTLDSILNIELIKDKDADEIREIWKQYFSLRNSVYAVIPGESFELIWRRAK 163

Query: 127 DCRHFVIPLWRGSGYTTMFVQVQMPYILFTGLEDYKARGTQAAPYFTVAYYKEFSESKDL 186
            C  F+  L R  GY     Q     + FT L + +  G  A     + +Y EF + K +
Sbjct: 164 TCPSFLYALPRKEGYEFFVGQWSGSELHFTALINIQTAGDAAPSQLILYHYPEFQKDKGI 223

Query: 187 VLIRGDIVFTSKLTDQEAEWLLETTQSFYLNDA--RYKLVERFNKQTHEFEFKDVLQALE 245
           VL+  +I  T  L  Q+A+ L    Q FY +D    + LVE+FN ++ EF++  V+  LE
Sbjct: 224 VLMTSEI-DTKFLNVQDAQCLANQVQLFYGSDGAETFGLVEKFNHKSDEFKYMAVVSFLE 282

[Close]

BLAST of Carg15327 vs. ExPASy Swiss-Prot
Match: Q5TC12 (ATP synthase mitochondrial F1 complex assembly factor 1 OS=Homo sapiens OX=9606 GN=ATPAF1 PE=1 SV=1)

HSP 1 Score: 85.9 bits (211), Expect = 7.1e-16
Identity = 54/173 (31.21%), Postives = 86/173 (49.71%), Query Frame = 0


Query: 74  QQKPLDSIMDMERAKDRSPEDLASIWDDYHLGRGHIGASMKAKLYHLLEQRAADCRHFVI 133
           + K L SI ++E  K+++ E++  IW  Y   +  + A + A+ + L+  RA  C  F+ 
Sbjct: 140 KDKTLSSIFNIEMVKEKTAEEIKQIWQQYFAAKDTVYAVIPAEKFDLIWNRAQSCPTFLC 199

Query: 134 PLWRGSGYTTMFVQVQMPYILFTGLEDYKARGTQAAPYFTVAYYKEFSESKDLVLIRGDI 193
            L R  GY     Q     + FT L + + RG  AA    + +Y E  E K +VL+  ++
Sbjct: 200 ALPRREGYEFFVGQWTGTELHFTALINIQTRGEAAASQLILYHYPELKEEKGIVLMTAEM 259

Query: 194 VFTSKLTDQEAEWLLETTQSFYLNDAR--YKLVERFNKQTHEFEFKDVLQALE 245
             T  L   EA+ +    Q FY  D +  Y LVE FN + +EF++  V+  LE
Sbjct: 260 DSTF-LNVAEAQCIANQVQLFYATDRKETYGLVETFNLRPNEFKYMSVIAELE 311

[Close]

BLAST of Carg15327 vs. ExPASy Swiss-Prot
Match: Q811I0 (ATP synthase mitochondrial F1 complex assembly factor 1 OS=Mus musculus OX=10090 GN=Atpaf1 PE=1 SV=1)

HSP 1 Score: 85.5 bits (210), Expect = 9.2e-16
Identity = 61/203 (30.05%), Postives = 97/203 (47.78%), Query Frame = 0


Query: 44  QKEAIPCDFLKWCSIGFFRTSRFASGFAPLQQKPLDSIMDMERAKDRSPEDLASIWDDYH 103
           Q + I C   K  ++G    S+   GF   + K L S+ ++E  KD++ E++  IW  Y 
Sbjct: 111 QSDFIKCMEQKTDALGKQPVSK---GFT--KDKTLSSVFNVEMVKDKTAEEIKQIWQQYF 170

Query: 104 LGRGHIGASMKAKLYHLLEQRAADCRHFVIPLWRGSGYTTMFVQVQMPYILFTGLEDYKA 163
             +  + A +  + + L+  RA  C  F+  L R  GY     Q     + FT L + + 
Sbjct: 171 SAKDTVYAVIPKEKFDLIWNRAQSCPTFLCALPRRDGYEFFVGQWTGTELHFTALINIQT 230

Query: 164 RGTQAAPYFTVAYYKEFSESKDLVLIRGDIVFTSKLTDQEAEWLLETTQSFYLNDAR--Y 223
           RG  AA    + +Y E  E K +VL+  ++  T  L   EA+ +    Q FY  D +  Y
Sbjct: 231 RGDAAASQLILYHYPELKEEKGIVLMTAEMDSTF-LNVVEAQCIANQVQLFYATDRKEIY 290

Query: 224 KLVERFNKQTHEFEFKDVLQALE 245
            LVE FN + +EF++  V+  LE
Sbjct: 291 GLVETFNFRPNEFKYMSVIAELE 307

[Close]

BLAST of Carg15327 vs. ExPASy Swiss-Prot
Match: P87127 (Protein atp11, mitochondrial OS=Schizosaccharomyces pombe (strain 972 / ATCC 24843) OX=284812 GN=atp11 PE=3 SV=1)

HSP 1 Score: 62.0 bits (149), Expect = 1.1e-08
Identity = 46/181 (25.41%), Postives = 86/181 (47.51%), Query Frame = 0


Query: 78  LDSIMDMERAKDRSPEDLASIWDDYHLGRGHIGASMKAKLYHLLEQRAADCRHFVIPLWR 137
           L+  +D+E+ K+     +  +W   ++G   + A +  ++Y  +  RA    +FV+PL R
Sbjct: 106 LNRYIDVEKIKELPTSTIEKLWRARNIGDDILSACIPKEIYEKMLSRARMYPYFVLPLPR 165

Query: 138 G-SGYTTMFVQVQMP-----YILFTGLEDYKARGTQAAPYFTVAYYKEFSESKDLVLIRG 197
           G  G  + F+Q   P     ++L T L +YK +G+ AAP+  + ++ +    K + L+R 
Sbjct: 166 GDKGIESHFLQWNFPNKNEAHLLVTSLLEYKLKGSYAAPHTIMLHFADLLNLKGITLMRC 225

Query: 198 DIVFTSKLTDQEAEWLLETTQSFY-------LNDARYKLVERFNKQTHEFEFKDVLQALE 246
                 KL+  + + L+   Q FY       L   R  L+  F+K   +F+   V   ++
Sbjct: 226 QFE-PKKLSANDVQLLVLAIQKFYNASENTPLGKERLALLAAFSKGA-DFDLHKVATHMD 284

[Close]

BLAST of Carg15327 vs. ExPASy TrEMBL
Match: A0A6J1EJC6 (ATP synthase mitochondrial F1 complex assembly factor 1 OS=Cucurbita moschata OX=3662 GN=LOC111433127 PE=3 SV=1)

HSP 1 Score: 492.7 bits (1267), Expect = 9.3e-136
Identity = 244/248 (98.39%), Postives = 246/248 (99.19%), Query Frame = 0


Query: 1   MRSSSLRPLRTIAGSAKFFLASASPAVRQSSRSYFANASLEKLQKEAIPCDFLKWCSIGF 60
           MRSSSLR LRTIAGSAKFFLASASPAVRQSSRSYFAN SLEKLQKEAIPCDFLKWCSIGF
Sbjct: 1   MRSSSLRTLRTIAGSAKFFLASASPAVRQSSRSYFANVSLEKLQKEAIPCDFLKWCSIGF 60

Query: 61  FRTSRFASGFAPLQQKPLDSIMDMERAKDRSPEDLASIWDDYHLGRGHIGASMKAKLYHL 120
           FRTSRFASGFAPLQQKPLDSIMDMERAKDRSPEDLASIWDDYHLGRGHIGASMKAKLYHL
Sbjct: 61  FRTSRFASGFAPLQQKPLDSIMDMERAKDRSPEDLASIWDDYHLGRGHIGASMKAKLYHL 120

Query: 121 LEQRAADCRHFVIPLWRGSGYTTMFVQVQMPYILFTGLEDYKARGTQAAPYFTVAYYKEF 180
           LEQRAADCRHFVIPLWRGSGYTTMFVQVQMPYILFTGLEDYKARGTQAAPYFTVAYYKEF
Sbjct: 121 LEQRAADCRHFVIPLWRGSGYTTMFVQVQMPYILFTGLEDYKARGTQAAPYFTVAYYKEF 180

Query: 181 SESKDLVLIRGDIVFTSKLTDQEAEWLLETTQSFYLNDARYKLVERFNKQTHEFEFKDVL 240
           +ESKDLVLIRGDIVFTSKLTD+EAEWLLETTQSFYLNDARYKLVERFNKQTHEFEFKDVL
Sbjct: 181 AESKDLVLIRGDIVFTSKLTDEEAEWLLETTQSFYLNDARYKLVERFNKQTHEFEFKDVL 240

Query: 241 QALEMPIL 249
           QALEMPIL
Sbjct: 241 QALEMPIL 248

[Close]

BLAST of Carg15327 vs. ExPASy TrEMBL
Match: A0A6J1IR42 (ATP synthase mitochondrial F1 complex assembly factor 1 OS=Cucurbita maxima OX=3661 GN=LOC111478614 PE=3 SV=1)

HSP 1 Score: 481.1 bits (1237), Expect = 2.8e-132
Identity = 239/248 (96.37%), Postives = 243/248 (97.98%), Query Frame = 0


Query: 1   MRSSSLRPLRTIAGSAKFFLASASPAVRQSSRSYFANASLEKLQKEAIPCDFLKWCSIGF 60
           MRSSSLR LRTIAGSAK FLASASPAVRQSSRSYFAN SLEKLQKEAIPCDFLKWCSIGF
Sbjct: 1   MRSSSLRTLRTIAGSAKNFLASASPAVRQSSRSYFANVSLEKLQKEAIPCDFLKWCSIGF 60

Query: 61  FRTSRFASGFAPLQQKPLDSIMDMERAKDRSPEDLASIWDDYHLGRGHIGASMKAKLYHL 120
           FRTSRFASGFAPLQQKPLDSIMDMERAKDRSPEDLASIWDDYHLGRGHIGASMKAKLYHL
Sbjct: 61  FRTSRFASGFAPLQQKPLDSIMDMERAKDRSPEDLASIWDDYHLGRGHIGASMKAKLYHL 120

Query: 121 LEQRAADCRHFVIPLWRGSGYTTMFVQVQMPYILFTGLEDYKARGTQAAPYFTVAYYKEF 180
           LEQRA+DCRHFVIPLWRGSGYTTMFVQVQMPYIL TGLEDYKARGTQAAPYFTVAYYKEF
Sbjct: 121 LEQRASDCRHFVIPLWRGSGYTTMFVQVQMPYILLTGLEDYKARGTQAAPYFTVAYYKEF 180

Query: 181 SESKDLVLIRGDIVFTSKLTDQEAEWLLETTQSFYLNDARYKLVERFNKQTHEFEFKDVL 240
           +ESKDLVLIRGDIVFTSKLTDQEAEWLLETTQSFYLNDARYKLVE+FNKQT +FEFKDVL
Sbjct: 181 AESKDLVLIRGDIVFTSKLTDQEAEWLLETTQSFYLNDARYKLVEQFNKQTRDFEFKDVL 240

Query: 241 QALEMPIL 249
           QALEMPIL
Sbjct: 241 QALEMPIL 248

[Close]

BLAST of Carg15327 vs. ExPASy TrEMBL
Match: A0A6J1CLK5 (ATP synthase mitochondrial F1 complex assembly factor 1 OS=Momordica charantia OX=3673 GN=LOC111012283 PE=3 SV=1)

HSP 1 Score: 442.6 bits (1137), Expect = 1.1e-120
Identity = 219/252 (86.90%), Postives = 236/252 (93.65%), Query Frame = 0


Query: 1   MRSSSLRPLRTIAGSAKFFLASASPAV---RQSSRSYFANASLEKLQ-KEAIPCDFLKWC 60
           MRSSS R LRTIAGSA+  LAS+SPAV    QSSR YFANASL+KLQ KEAIPCDF+KWC
Sbjct: 1   MRSSSFRTLRTIAGSARSLLASSSPAVPCLGQSSRCYFANASLQKLQKKEAIPCDFIKWC 60

Query: 61  SIGFFRTSRFASGFAPLQQKPLDSIMDMERAKDRSPEDLASIWDDYHLGRGHIGASMKAK 120
           S+GFFRTS+FA+GF PLQ KPLDSI+DMERAKDRSPEDLASIWDDYHLGRGHIGASMKAK
Sbjct: 61  SLGFFRTSKFATGFTPLQPKPLDSIIDMERAKDRSPEDLASIWDDYHLGRGHIGASMKAK 120

Query: 121 LYHLLEQRAADCRHFVIPLWRGSGYTTMFVQVQMPYILFTGLEDYKARGTQAAPYFTVAY 180
           LYHLLEQRA+DCR+FV+PLWRGSGYTTMF+QV MP+IL TGLEDYKARGTQAAPYFTV+Y
Sbjct: 121 LYHLLEQRASDCRYFVVPLWRGSGYTTMFIQVHMPHILITGLEDYKARGTQAAPYFTVSY 180

Query: 181 YKEFSESKDLVLIRGDIVFTSKLTDQEAEWLLETTQSFYLNDARYKLVERFNKQTHEFEF 240
           YKEF+ESKDLVLIRGDIVFTSKLTDQEAEWLLETTQSFYLND RYKLVERFN+QT +FEF
Sbjct: 181 YKEFAESKDLVLIRGDIVFTSKLTDQEAEWLLETTQSFYLNDVRYKLVERFNRQTRDFEF 240

Query: 241 KDVLQALEMPIL 249
           KDVLQALEMPIL
Sbjct: 241 KDVLQALEMPIL 252

[Close]

BLAST of Carg15327 vs. ExPASy TrEMBL
Match: A0A1S3AZK5 (ATP synthase mitochondrial F1 complex assembly factor 1 OS=Cucumis melo OX=3656 GN=LOC103484474 PE=3 SV=1)

HSP 1 Score: 439.1 bits (1128), Expect = 1.2e-119
Identity = 216/248 (87.10%), Postives = 231/248 (93.15%), Query Frame = 0


Query: 1   MRSSSLRPLRTIAGSAKFFLASASPAVRQSSRSYFANASLEKLQKEAIPCDFLKWCSIGF 60
           MRSSSLR LRTIAGS K  LAS+SP VRQSSRSYFAN SL KLQKEA P DFLKW S+GF
Sbjct: 1   MRSSSLRTLRTIAGSVKTVLASSSPVVRQSSRSYFANLSLGKLQKEANPSDFLKWSSLGF 60

Query: 61  FRTSRFASGFAPLQQKPLDSIMDMERAKDRSPEDLASIWDDYHLGRGHIGASMKAKLYHL 120
           FRTS+FA+GF PLQ KPL+SI+DMER KDRSPEDLASIWDDYHLGRGHIG SMKAKLYHL
Sbjct: 61  FRTSKFATGFTPLQPKPLNSIIDMERVKDRSPEDLASIWDDYHLGRGHIGISMKAKLYHL 120

Query: 121 LEQRAADCRHFVIPLWRGSGYTTMFVQVQMPYILFTGLEDYKARGTQAAPYFTVAYYKEF 180
           LE RAADCR+FVIPLWRGSGYTTMFVQVQMP+I+FTGLEDYKARGTQAAPYFTV+YYKEF
Sbjct: 121 LEHRAADCRYFVIPLWRGSGYTTMFVQVQMPHIIFTGLEDYKARGTQAAPYFTVSYYKEF 180

Query: 181 SESKDLVLIRGDIVFTSKLTDQEAEWLLETTQSFYLNDARYKLVERFNKQTHEFEFKDVL 240
           +ESKDLVLIRGDIVFTSKLTD+EAEWLLETTQSFYLND RYKLVERFN+QT +FEFKDVL
Sbjct: 181 AESKDLVLIRGDIVFTSKLTDEEAEWLLETTQSFYLNDVRYKLVERFNRQTRDFEFKDVL 240

Query: 241 QALEMPIL 249
           QAL+MPIL
Sbjct: 241 QALDMPIL 248

[Close]

BLAST of Carg15327 vs. ExPASy TrEMBL
Match: A0A0A0KI74 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_6G518330 PE=3 SV=1)

HSP 1 Score: 437.6 bits (1124), Expect = 3.5e-119
Identity = 215/248 (86.69%), Postives = 231/248 (93.15%), Query Frame = 0


Query: 1   MRSSSLRPLRTIAGSAKFFLASASPAVRQSSRSYFANASLEKLQKEAIPCDFLKWCSIGF 60
           MRSSSL+ LRTIAGS K  +AS+SP VRQSSRSYFAN SL KLQKE  P DFLKW S+GF
Sbjct: 1   MRSSSLQTLRTIAGSVKTVIASSSPVVRQSSRSYFANPSLGKLQKETNPSDFLKWSSLGF 60

Query: 61  FRTSRFASGFAPLQQKPLDSIMDMERAKDRSPEDLASIWDDYHLGRGHIGASMKAKLYHL 120
           FRTS+FA+GF PLQ KPLDSI+DMERAKDRSPEDLASIWDDYHLGRGHIG SMKAKLYHL
Sbjct: 61  FRTSKFATGFNPLQPKPLDSIIDMERAKDRSPEDLASIWDDYHLGRGHIGISMKAKLYHL 120

Query: 121 LEQRAADCRHFVIPLWRGSGYTTMFVQVQMPYILFTGLEDYKARGTQAAPYFTVAYYKEF 180
           LEQRAADCR+FVIPLWRGSGYTTMFVQVQ P+I+FTGLEDYKARGTQAAPYFTV+YYKEF
Sbjct: 121 LEQRAADCRYFVIPLWRGSGYTTMFVQVQTPHIIFTGLEDYKARGTQAAPYFTVSYYKEF 180

Query: 181 SESKDLVLIRGDIVFTSKLTDQEAEWLLETTQSFYLNDARYKLVERFNKQTHEFEFKDVL 240
           +ESKDLVLIRGDIVFTSKLTD+EAEWLLETTQSFYLND RYKLVERFN+QT +FEFKDVL
Sbjct: 181 AESKDLVLIRGDIVFTSKLTDEEAEWLLETTQSFYLNDVRYKLVERFNRQTRDFEFKDVL 240

Query: 241 QALEMPIL 249
           QAL+MPIL
Sbjct: 241 QALDMPIL 248

[Close]

BLAST of Carg15327 vs. TAIR 10
Match: AT2G34050.1 (INVOLVED IN: protein complex assembly; LOCATED IN: mitochondrion, chloroplast; EXPRESSED IN: 19 plant structures; EXPRESSED DURING: 9 growth stages; CONTAINS InterPro DOMAIN/s: ATP11 (InterPro:IPR010591); Has 304 Blast hits to 304 proteins in 167 species: Archae - 0; Bacteria - 0; Metazoa - 101; Fungi - 112; Plants - 39; Viruses - 0; Other Eukaryotes - 52 (source: NCBI BLink). )

HSP 1 Score: 333.2 bits (853), Expect = 1.8e-91
Identity = 163/249 (65.46%), Postives = 199/249 (79.92%), Query Frame = 0


Query: 7   RPLRTIAGSAKFFLASASPAVRQSSRSYFANAS-------LEKLQKEAIPCDFLKWCSIG 66
           R + +I+  AK  L+S+S     SSR  F   S         KL + ++P + +KW S+G
Sbjct: 3   RIVGSISSLAKDKLSSSS---YSSSRQIFTARSSSWQRDASSKLSEASLPGNHIKWASLG 62

Query: 67  FFRTSRFASGFAPLQQKPLDSIMDMERAKDRSPEDLASIWDDYHLGRGHIGASMKAKLYH 126
             R SRFASGF PLQQKPLDSIMD+ RAK +SPE+L SIWDDYHLGRGHIG +MKA+LY 
Sbjct: 63  SVRNSRFASGFTPLQQKPLDSIMDLARAKTKSPEELTSIWDDYHLGRGHIGLTMKAQLYR 122

Query: 127 LLEQRAADCRHFVIPLWRGSGYTTMFVQVQMPYILFTGLEDYKARGTQAAPYFTVAYYKE 186
           LLEQRA++CR+FVIPLWRG+GY TMF QV+ P+++FTGLEDYKARGTQAAPY T  +Y E
Sbjct: 123 LLEQRASECRYFVIPLWRGNGYITMFAQVEAPHMIFTGLEDYKARGTQAAPYLTTTFYTE 182

Query: 187 FSESKDLVLIRGDIVFTSKLTDQEAEWLLETTQSFYLNDARYKLVERFNKQTHEFEFKDV 246
            SE+KDLV IRGD+VFTSKLTD+EA+W++ET QSFYLND+RYKL+ERFNK TH+FEFKDV
Sbjct: 183 LSETKDLVFIRGDVVFTSKLTDEEAKWIMETAQSFYLNDSRYKLLERFNKHTHDFEFKDV 242

Query: 247 LQALEMPIL 249
           LQAL+MP+L
Sbjct: 243 LQALDMPLL 248

The following BLAST results are available for this feature:

BLAST of Carg15327 vs. NCBI nr


Match Name	E-value	Identity	Description
KAG7034627.1	1.6e-137	100.00	ATP synthase mitochondrial F1 complex assembly factor 1 [Cucurbita argyrosperma ...	[more]
XP_022925840.1	1.9e-135	98.39	ATP synthase mitochondrial F1 complex assembly factor 1 [Cucurbita moschata]	[more]
XP_023544293.1	2.3e-133	97.18	ATP synthase mitochondrial F1 complex assembly factor 1 [Cucurbita pepo subsp. p...	[more]
XP_022978750.1	5.8e-132	96.37	ATP synthase mitochondrial F1 complex assembly factor 1 [Cucurbita maxima]	[more]
XP_038883536.1	2.0e-124	89.92	ATP synthase mitochondrial F1 complex assembly factor 1 [Benincasa hispida]	[more]

BLAST of Carg15327 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q66JD1	3.2e-16	31.67	ATP synthase mitochondrial F1 complex assembly factor 1 OS=Xenopus tropicalis OX...	[more]
Q5TC12	7.1e-16	31.21	ATP synthase mitochondrial F1 complex assembly factor 1 OS=Homo sapiens OX=9606 ...	[more]
Q811I0	9.2e-16	30.05	ATP synthase mitochondrial F1 complex assembly factor 1 OS=Mus musculus OX=10090...	[more]
P87127	1.1e-08	25.41	Protein atp11, mitochondrial OS=Schizosaccharomyces pombe (strain 972 / ATCC 248...	[more]

BLAST of Carg15327 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1EJC6	9.3e-136	98.39	ATP synthase mitochondrial F1 complex assembly factor 1 OS=Cucurbita moschata OX...	[more]
A0A6J1IR42	2.8e-132	96.37	ATP synthase mitochondrial F1 complex assembly factor 1 OS=Cucurbita maxima OX=3...	[more]
A0A6J1CLK5	1.1e-120	86.90	ATP synthase mitochondrial F1 complex assembly factor 1 OS=Momordica charantia O...	[more]
A0A1S3AZK5	1.2e-119	87.10	ATP synthase mitochondrial F1 complex assembly factor 1 OS=Cucumis melo OX=3656 ...	[more]
A0A0A0KI74	3.5e-119	86.69	Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_6G518330 PE=3 SV=1	[more]

BLAST of Carg15327 vs. TAIR 10


Match Name	E-value	Identity	Description
AT2G34050.1	1.8e-91	65.46	INVOLVED IN: protein complex assembly; LOCATED IN: mitochondrion, chloroplast; E...	[more]

InterPro

Analysis Name: InterPro Annotations of Silver-seed gourd (SMH-JMG-627) v2
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR010591	ATP11	PFAM	PF06644	ATP11	coord: 72..239 e-value: 2.0E-38 score: 132.7
IPR010591	ATP11	PANTHER	PTHR13126	CHAPERONE ATP11	coord: 23..248
None	No IPR available	PANTHER	PTHR13126:SF1	BNAA04G19940D PROTEIN	coord: 23..248

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Carg15327-RA	Carg15327-RA	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0033615	mitochondrial proton-transporting ATP synthase complex assembly
biological_process	GO:0065003	protein-containing complex assembly
cellular_component	GO:0009570	chloroplast stroma
cellular_component	GO:0005739	mitochondrion