Carg15326 (gene) Silver-seed gourd (SMH-JMG-627) v2

BLAST of Carg15326 vs. NCBI nr
Match: KAG7034626.1 (GDSL esterase/lipase [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 493.4 bits (1269), Expect = 1.1e-135
Identity = 237/237 (100.00%), Postives = 237/237 (100.00%), Query Frame = 0

Query: 1   MCCWQNVIPLRKQLDYLKEYKKRVELGMGKDRAEAHIKKAAFVVSAGTNDFVLNYFLLPF 60
           MCCWQNVIPLRKQLDYLKEYKKRVELGMGKDRAEAHIKKAAFVVSAGTNDFVLNYFLLPF
Sbjct: 1   MCCWQNVIPLRKQLDYLKEYKKRVELGMGKDRAEAHIKKAAFVVSAGTNDFVLNYFLLPF 60

Query: 61  RRQIYSVSSYQRFVIQLLMDFLQGLWEEGGRRIAVVGLPPIGCLPIVITLNLDKTTLTRG 120
           RRQIYSVSSYQRFVIQLLMDFLQGLWEEGGRRIAVVGLPPIGCLPIVITLNLDKTTLTRG
Sbjct: 61  RRQIYSVSSYQRFVIQLLMDFLQGLWEEGGRRIAVVGLPPIGCLPIVITLNLDKTTLTRG 120

Query: 121 CVESYSAAARGFNQILKKELKSMQSKLAKDNVKFYYVDSYGPLADMIHNFHNYGFEEAGS 180
           CVESYSAAARGFNQILKKELKSMQSKLAKDNVKFYYVDSYGPLADMIHNFHNYGFEEAGS
Sbjct: 121 CVESYSAAARGFNQILKKELKSMQSKLAKDNVKFYYVDSYGPLADMIHNFHNYGFEEAGS 180

Query: 181 GCCGSGYVEASFLCNPKTYTCPDASKYVFWDSIHPTQATYHNIFLAGRPIVDAMLKH 238
           GCCGSGYVEASFLCNPKTYTCPDASKYVFWDSIHPTQATYHNIFLAGRPIVDAMLKH
Sbjct: 181 GCCGSGYVEASFLCNPKTYTCPDASKYVFWDSIHPTQATYHNIFLAGRPIVDAMLKH 237

BLAST of Carg15326 vs. NCBI nr
Match: KAG6581342.1 (GDSL esterase/lipase, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 478.8 bits (1231), Expect = 2.7e-131
Identity = 232/232 (100.00%), Postives = 232/232 (100.00%), Query Frame = 0

Query: 6   NVIPLRKQLDYLKEYKKRVELGMGKDRAEAHIKKAAFVVSAGTNDFVLNYFLLPFRRQIY 65
           NVIPLRKQLDYLKEYKKRVELGMGKDRAEAHIKKAAFVVSAGTNDFVLNYFLLPFRRQIY
Sbjct: 142 NVIPLRKQLDYLKEYKKRVELGMGKDRAEAHIKKAAFVVSAGTNDFVLNYFLLPFRRQIY 201

Query: 66  SVSSYQRFVIQLLMDFLQGLWEEGGRRIAVVGLPPIGCLPIVITLNLDKTTLTRGCVESY 125
           SVSSYQRFVIQLLMDFLQGLWEEGGRRIAVVGLPPIGCLPIVITLNLDKTTLTRGCVESY
Sbjct: 202 SVSSYQRFVIQLLMDFLQGLWEEGGRRIAVVGLPPIGCLPIVITLNLDKTTLTRGCVESY 261

Query: 126 SAAARGFNQILKKELKSMQSKLAKDNVKFYYVDSYGPLADMIHNFHNYGFEEAGSGCCGS 185
           SAAARGFNQILKKELKSMQSKLAKDNVKFYYVDSYGPLADMIHNFHNYGFEEAGSGCCGS
Sbjct: 262 SAAARGFNQILKKELKSMQSKLAKDNVKFYYVDSYGPLADMIHNFHNYGFEEAGSGCCGS 321

Query: 186 GYVEASFLCNPKTYTCPDASKYVFWDSIHPTQATYHNIFLAGRPIVDAMLKH 238
           GYVEASFLCNPKTYTCPDASKYVFWDSIHPTQATYHNIFLAGRPIVDAMLKH
Sbjct: 322 GYVEASFLCNPKTYTCPDASKYVFWDSIHPTQATYHNIFLAGRPIVDAMLKH 373

BLAST of Carg15326 vs. NCBI nr
Match: XP_022925836.1 (GDSL esterase/lipase At5g45960-like isoform X1 [Cucurbita moschata])

HSP 1 Score: 476.5 bits (1225), Expect = 1.4e-130
Identity = 231/232 (99.57%), Postives = 231/232 (99.57%), Query Frame = 0

Query: 6   NVIPLRKQLDYLKEYKKRVELGMGKDRAEAHIKKAAFVVSAGTNDFVLNYFLLPFRRQIY 65
           NVIPLRKQLDYLKEYKKRVELGMGKDRAEAHIKKAAFVVSAGTNDFVLNYFLLPFRRQIY
Sbjct: 146 NVIPLRKQLDYLKEYKKRVELGMGKDRAEAHIKKAAFVVSAGTNDFVLNYFLLPFRRQIY 205

Query: 66  SVSSYQRFVIQLLMDFLQGLWEEGGRRIAVVGLPPIGCLPIVITLNLDKTTLTRGCVESY 125
           SVSSYQRFVIQLLMDFLQGLWEEGGRRIAVVGLPPIGCLPIVITLNLDKT LTRGCVESY
Sbjct: 206 SVSSYQRFVIQLLMDFLQGLWEEGGRRIAVVGLPPIGCLPIVITLNLDKTMLTRGCVESY 265

Query: 126 SAAARGFNQILKKELKSMQSKLAKDNVKFYYVDSYGPLADMIHNFHNYGFEEAGSGCCGS 185
           SAAARGFNQILKKELKSMQSKLAKDNVKFYYVDSYGPLADMIHNFHNYGFEEAGSGCCGS
Sbjct: 266 SAAARGFNQILKKELKSMQSKLAKDNVKFYYVDSYGPLADMIHNFHNYGFEEAGSGCCGS 325

Query: 186 GYVEASFLCNPKTYTCPDASKYVFWDSIHPTQATYHNIFLAGRPIVDAMLKH 238
           GYVEASFLCNPKTYTCPDASKYVFWDSIHPTQATYHNIFLAGRPIVDAMLKH
Sbjct: 326 GYVEASFLCNPKTYTCPDASKYVFWDSIHPTQATYHNIFLAGRPIVDAMLKH 377

BLAST of Carg15326 vs. NCBI nr
Match: XP_023544289.1 (GDSL esterase/lipase At5g45960-like isoform X1 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 465.7 bits (1197), Expect = 2.4e-127
Identity = 225/232 (96.98%), Postives = 228/232 (98.28%), Query Frame = 0

Query: 6   NVIPLRKQLDYLKEYKKRVELGMGKDRAEAHIKKAAFVVSAGTNDFVLNYFLLPFRRQIY 65
           NVIPLRKQLDYLKEYKKRVE G+GKDRAEAHIKKAAFVVSAGTNDFVLNYFLLPFRRQIY
Sbjct: 146 NVIPLRKQLDYLKEYKKRVESGIGKDRAEAHIKKAAFVVSAGTNDFVLNYFLLPFRRQIY 205

Query: 66  SVSSYQRFVIQLLMDFLQGLWEEGGRRIAVVGLPPIGCLPIVITLNLDKTTLTRGCVESY 125
           SVSSYQRFVIQLLMDFLQGLWEEGGRRIAVVGLPPIGCLPIVITLN DKTTLTRGCV+SY
Sbjct: 206 SVSSYQRFVIQLLMDFLQGLWEEGGRRIAVVGLPPIGCLPIVITLNFDKTTLTRGCVDSY 265

Query: 126 SAAARGFNQILKKELKSMQSKLAKDNVKFYYVDSYGPLADMIHNFHNYGFEEAGSGCCGS 185
           SAAARGFNQILKKELKSMQSKLAKDNVK YYVDSYGPLADMIHNFHNYGFEEAGSGCCGS
Sbjct: 266 SAAARGFNQILKKELKSMQSKLAKDNVKLYYVDSYGPLADMIHNFHNYGFEEAGSGCCGS 325

Query: 186 GYVEASFLCNPKTYTCPDASKYVFWDSIHPTQATYHNIFLAGRPIVDAMLKH 238
           GYVEASFLCNPKTYTCPDASKYVFWDSIHPTQ TYHNIFLAGRPIVDAMLK+
Sbjct: 326 GYVEASFLCNPKTYTCPDASKYVFWDSIHPTQTTYHNIFLAGRPIVDAMLKN 377

BLAST of Carg15326 vs. NCBI nr
Match: XP_022978719.1 (GDSL esterase/lipase At5g45960-like isoform X1 [Cucurbita maxima])

HSP 1 Score: 460.7 bits (1184), Expect = 7.7e-126
Identity = 223/232 (96.12%), Postives = 228/232 (98.28%), Query Frame = 0

Query: 6   NVIPLRKQLDYLKEYKKRVELGMGKDRAEAHIKKAAFVVSAGTNDFVLNYFLLPFRRQIY 65
           NVIP+RKQLDYLKEYKKRVELG+GKDRAEAH+KKAAFVVSAGTNDFVLNYFLLPFRRQIY
Sbjct: 146 NVIPVRKQLDYLKEYKKRVELGIGKDRAEAHMKKAAFVVSAGTNDFVLNYFLLPFRRQIY 205

Query: 66  SVSSYQRFVIQLLMDFLQGLWEEGGRRIAVVGLPPIGCLPIVITLNLDKTTLTRGCVESY 125
           SVSSYQRFVIQLLMDFLQGLWEEGGRRIAVVGLPPIGCLPIVITLNLDKTTL RGCVESY
Sbjct: 206 SVSSYQRFVIQLLMDFLQGLWEEGGRRIAVVGLPPIGCLPIVITLNLDKTTLPRGCVESY 265

Query: 126 SAAARGFNQILKKELKSMQSKLAKDNVKFYYVDSYGPLADMIHNFHNYGFEEAGSGCCGS 185
           SAAARGFNQIL+ ELKSMQSKLAKDNVKF+YVDSY PLADMIHNFHNYGFEEAGSGCCGS
Sbjct: 266 SAAARGFNQILRMELKSMQSKLAKDNVKFFYVDSYEPLADMIHNFHNYGFEEAGSGCCGS 325

Query: 186 GYVEASFLCNPKTYTCPDASKYVFWDSIHPTQATYHNIFLAGRPIVDAMLKH 238
           GYVEASFLCNPKT TCPDASKYVFWDSIHPTQATYHNIFLAGRPIVDAMLKH
Sbjct: 326 GYVEASFLCNPKTDTCPDASKYVFWDSIHPTQATYHNIFLAGRPIVDAMLKH 377

BLAST of Carg15326 vs. ExPASy Swiss-Prot
Match: Q9FJ40 (GDSL esterase/lipase At5g45960 OS=Arabidopsis thaliana OX=3702 GN=At5g45960 PE=2 SV=1)

HSP 1 Score: 265.0 bits (676), Expect = 8.1e-70
Identity = 122/230 (53.04%), Postives = 167/230 (72.61%), Query Frame = 0

Query: 6   NVIPLRKQLDYLKEYKKRVELGMGKDRAEAHIKKAAFVVSAGTNDFVLNYFLLPFRRQIY 65
           NVI +  QL+Y +EYK+++E  MGK   E HI++A F VSAGTNDFV+NYF +P RR+ +
Sbjct: 143 NVIDIPTQLEYFREYKRKLEGKMGKQEMEKHIEEAMFCVSAGTNDFVINYFTIPIRRKTF 202

Query: 66  SVSSYQRFVIQLLMDFLQGLWEEGGRRIAVVGLPPIGCLPIVITLNLDKTTLTRGCVESY 125
           ++ +YQ+FVI  L  F+QGLW+EG R+I V GLPPIGCLPIVITL   +    R C++ +
Sbjct: 203 TIEAYQQFVISNLKQFIQGLWKEGARKITVAGLPPIGCLPIVITLFSGEALTNRRCIDRF 262

Query: 126 SAAARGFNQILKKELKSMQSKLAKDNVKFYYVDSYGPLADMIHNFHNYGFEEAGSGCCGS 185
           S  A  +N +L+K+L  MQ  LA    K +Y+D Y P+ ++I +   +GFEE  SGCCGS
Sbjct: 263 STVATNYNFLLQKQLALMQVGLAHLGSKIFYLDVYNPVYEVIRDPRKFGFEEVFSGCCGS 322

Query: 186 GYVEASFLCNPKTYTCPDASKYVFWDSIHPTQATYHNIFLAGRPIVDAML 236
           GY+EASFLCNPK+Y CP+ S YVF+DSIHP++ TY ++F + RPI D++L
Sbjct: 323 GYLEASFLCNPKSYVCPNTSAYVFFDSIHPSEKTYFSLFRSLRPIYDSIL 372

BLAST of Carg15326 vs. ExPASy Swiss-Prot
Match: Q9LMJ3 (GDSL esterase/lipase At1g06990 OS=Arabidopsis thaliana OX=3702 GN=At1g06990 PE=2 SV=2)

HSP 1 Score: 179.9 bits (455), Expect = 3.4e-44
Identity = 90/221 (40.72%), Postives = 137/221 (61.99%), Query Frame = 0

Query: 6   NVIPLRKQLDYLKEYKKRVELGMGKDRAEAHIKKAAFVVSAGTNDFVLNYFLLPFRRQIY 65
           + + + KQ D L+ Y +R+   +G ++A + + +A  +VS+GTNDF LN +  P RRQ  
Sbjct: 133 STLSVDKQADMLRSYVERLSQIVGDEKAASIVSEALVIVSSGTNDFNLNLYDTPSRRQKL 192

Query: 66  SVSSYQRFVIQLLMDFLQGLWEEGGRRIAVVGLPPIGCLPIVITLNLDKTTLTRGCVESY 125
            V  YQ F++  + +F+Q L++ G R+I V+GLPP+GCLPI +T+ + K    R C++  
Sbjct: 193 GVDGYQSFILSNVHNFVQELYDIGCRKIMVLGLPPVGCLPIQMTMAMQKQN-ERRCIDKQ 252

Query: 126 SAAARGFNQILKKELKSMQSKLAKDNVKFYYVDSYGPLADMIHNFHNYGFEEAGSGCCGS 185
           ++ ++ FNQ LK  L  MQS L    +  +Y D YG L DM  N   YG +E   GCCG+
Sbjct: 253 NSDSQEFNQKLKNSLTEMQSNLTGSVI--FYGDIYGALFDMATNPQRYGLKETTRGCCGT 312

Query: 186 GYVEASFLCNPKTYTCPDASKYVFWDSIHPTQATYHNIFLA 227
           G +E ++LCN  T  CP+ ++Y+FWD IHP+Q  Y  I L+
Sbjct: 313 GEIELAYLCNALTRICPNPNQYLFWDDIHPSQIAYIVISLS 350

BLAST of Carg15326 vs. ExPASy Swiss-Prot
Match: O22927 (GDSL esterase/lipase At2g30310 OS=Arabidopsis thaliana OX=3702 GN=At2g30310 PE=2 SV=1)

HSP 1 Score: 178.3 bits (451), Expect = 1.0e-43
Identity = 94/217 (43.32%), Postives = 135/217 (62.21%), Query Frame = 0

Query: 8   IPLRKQLDYLKEYKKRVELGMGKDRAEAHIKKAAFVVSAGTNDFVLNYFLLPFRRQIY-S 67
           IP+ +Q    K Y  R++  +G  +A   I  A  V+SAG NDF+LN++ +P RR  Y +
Sbjct: 133 IPVSQQPSMFKNYIARLKGIVGDKKAMEIINNALVVISAGPNDFILNFYDIPTRRLEYPT 192

Query: 68  VSSYQRFVIQLLMDFLQGLWEEGGRRIAVVGLPPIGCLPIVITLNLDKTTLTRGCVESYS 127
           +  YQ F+++ L  F++ L+  G R I V GLPP+GCLPI +T  +    + R CVE  +
Sbjct: 193 IHGYQEFILKRLDGFVRELYSLGCRNIVVGGLPPMGCLPIQMTAKM--RNILRFCVEQEN 252

Query: 128 AAARGFNQILKKELKSMQSKLAKDNVKFYYVDSYGPLADMIHNFHNYGFEEAGSGCCGSG 187
             +  +NQ L K+L  +Q+ L   N  F Y + Y PL DMI N   YGF+E   GCCG+G
Sbjct: 253 KDSVLYNQKLVKKLPEIQASLPGSN--FLYANVYDPLMDMIQNPSKYGFKETKKGCCGTG 312

Query: 188 YVEASFLCNPKTYTCPDASKYVFWDSIHPTQATYHNI 224
           Y+E +F+CNP T TCP+ S ++FWDSIHP++A Y+ I
Sbjct: 313 YLETTFMCNPLTKTCPNHSDHLFWDSIHPSEAAYNYI 345

BLAST of Carg15326 vs. ExPASy Swiss-Prot
Match: Q8VY93 (GDSL esterase/lipase At4g26790 OS=Arabidopsis thaliana OX=3702 GN=At4g26790 PE=2 SV=1)

HSP 1 Score: 171.8 bits (434), Expect = 9.4e-42
Identity = 85/215 (39.53%), Postives = 136/215 (63.26%), Query Frame = 0

Query: 6   NVIPLRKQLDYLKEYKKRVELGMGKDRAEAHIKKAAFVVSAGTNDFVLNYFLLPFRRQIY 65
           +V+PL K+++Y KEY+ R+   +G+++A   I ++ +++S GTNDF+ NY+LLP + + Y
Sbjct: 125 SVMPLWKEVEYYKEYQTRLRSYLGEEKANEIISESLYLISIGTNDFLENYYLLPRKLRKY 184

Query: 66  SVSSYQRFVIQLLMDFLQGLWEEGGRRIAVVGLPPIGCLPIVITLNLDKTTLTRGCVESY 125
           SV+ YQ F+I +  DF+  ++  G R++++ GL P GCLP+  T  L        C+E Y
Sbjct: 185 SVNEYQYFLIGIAADFVTDIYRLGARKMSLSGLSPFGCLPLERTTQL---FYGSKCIEEY 244

Query: 126 SAAARGFNQILKKELKSMQSKLAKDNVKFYYVDSYGPLADMIHNFHNYGFEEAGSGCCGS 185
           +  AR FN  +K E K  Q     + ++  + + Y  ++++I++   +GFE   S CCG+
Sbjct: 245 NIVARDFN--IKMEEKVFQLNRDLNGIQLVFSNPYDLVSEIIYHPEAFGFENVRSACCGT 304

Query: 186 GYVEASFLCNP-KTYTCPDASKYVFWDSIHPTQAT 220
           GY E S+LC+    +TC DASKYVFWDS HPT+ T
Sbjct: 305 GYYEMSYLCDKMNPFTCSDASKYVFWDSFHPTEKT 334

BLAST of Carg15326 vs. ExPASy Swiss-Prot
Match: O22918 (GDSL esterase/lipase At2g30220 OS=Arabidopsis thaliana OX=3702 GN=At2g30220 PE=3 SV=1)

HSP 1 Score: 170.2 bits (430), Expect = 2.7e-41
Identity = 93/214 (43.46%), Postives = 131/214 (61.21%), Query Frame = 0

Query: 8   IPLRKQLDYLKEYKKRVELGMGKDRAEAHIKKAAFVVSAGTNDFVLNYFLLPFRRQIY-S 67
           IP+ +Q    K Y  R++  +G  +A   I  A  V+SAG NDF+LN++ +P RR  Y +
Sbjct: 132 IPVSQQPSMFKNYIARLKGIVGDKKAMEIINNALVVISAGPNDFILNFYDIPIRRLEYPT 191

Query: 68  VSSYQRFVIQLLMDFLQGLWEEGGRRIAVVGLPPIGCLPIVITLNLDKTTLTRGCVESYS 127
           +  YQ FV++ L  F++ L+  G R I V GLPP+GCLPI +T  L   T+   CVE  +
Sbjct: 192 IYGYQDFVLKRLDGFVRELYSLGCRNILVGGLPPMGCLPIQLTAKL--RTILGICVEQEN 251

Query: 128 AAARGFNQILKKELKSMQSKLAKDNVKFYYVDSYGPLADMIHNFHNYGFEEAGSGCCGSG 187
             +  +NQ L K+L  +Q+ L     KF Y + Y P+ DMI N   YGF+E   GCCG+G
Sbjct: 252 KDSILYNQKLVKKLPEIQASL--PGSKFLYANVYDPVMDMIRNPSKYGFKETKKGCCGTG 311

Query: 188 YVEASFLCNPKTYTCPDASKYVFWDSIHPTQATY 221
           Y+E SFLC   + TCP+ S ++FWDSIHP++A Y
Sbjct: 312 YLETSFLCTSLSKTCPNHSDHLFWDSIHPSEAAY 341

BLAST of Carg15326 vs. ExPASy TrEMBL
Match: A0A6J1ED93 (GDSL esterase/lipase At5g45960-like isoform X1 OS=Cucurbita moschata OX=3662 GN=LOC111433124 PE=3 SV=1)

HSP 1 Score: 476.5 bits (1225), Expect = 6.6e-131
Identity = 231/232 (99.57%), Postives = 231/232 (99.57%), Query Frame = 0

Query: 6   NVIPLRKQLDYLKEYKKRVELGMGKDRAEAHIKKAAFVVSAGTNDFVLNYFLLPFRRQIY 65
           NVIPLRKQLDYLKEYKKRVELGMGKDRAEAHIKKAAFVVSAGTNDFVLNYFLLPFRRQIY
Sbjct: 146 NVIPLRKQLDYLKEYKKRVELGMGKDRAEAHIKKAAFVVSAGTNDFVLNYFLLPFRRQIY 205

Query: 66  SVSSYQRFVIQLLMDFLQGLWEEGGRRIAVVGLPPIGCLPIVITLNLDKTTLTRGCVESY 125
           SVSSYQRFVIQLLMDFLQGLWEEGGRRIAVVGLPPIGCLPIVITLNLDKT LTRGCVESY
Sbjct: 206 SVSSYQRFVIQLLMDFLQGLWEEGGRRIAVVGLPPIGCLPIVITLNLDKTMLTRGCVESY 265

Query: 126 SAAARGFNQILKKELKSMQSKLAKDNVKFYYVDSYGPLADMIHNFHNYGFEEAGSGCCGS 185
           SAAARGFNQILKKELKSMQSKLAKDNVKFYYVDSYGPLADMIHNFHNYGFEEAGSGCCGS
Sbjct: 266 SAAARGFNQILKKELKSMQSKLAKDNVKFYYVDSYGPLADMIHNFHNYGFEEAGSGCCGS 325

Query: 186 GYVEASFLCNPKTYTCPDASKYVFWDSIHPTQATYHNIFLAGRPIVDAMLKH 238
           GYVEASFLCNPKTYTCPDASKYVFWDSIHPTQATYHNIFLAGRPIVDAMLKH
Sbjct: 326 GYVEASFLCNPKTYTCPDASKYVFWDSIHPTQATYHNIFLAGRPIVDAMLKH 377

BLAST of Carg15326 vs. ExPASy TrEMBL
Match: A0A6J1IUT9 (GDSL esterase/lipase At5g45960-like isoform X1 OS=Cucurbita maxima OX=3661 GN=LOC111478591 PE=3 SV=1)

HSP 1 Score: 460.7 bits (1184), Expect = 3.7e-126
Identity = 223/232 (96.12%), Postives = 228/232 (98.28%), Query Frame = 0

Query: 6   NVIPLRKQLDYLKEYKKRVELGMGKDRAEAHIKKAAFVVSAGTNDFVLNYFLLPFRRQIY 65
           NVIP+RKQLDYLKEYKKRVELG+GKDRAEAH+KKAAFVVSAGTNDFVLNYFLLPFRRQIY
Sbjct: 146 NVIPVRKQLDYLKEYKKRVELGIGKDRAEAHMKKAAFVVSAGTNDFVLNYFLLPFRRQIY 205

Query: 66  SVSSYQRFVIQLLMDFLQGLWEEGGRRIAVVGLPPIGCLPIVITLNLDKTTLTRGCVESY 125
           SVSSYQRFVIQLLMDFLQGLWEEGGRRIAVVGLPPIGCLPIVITLNLDKTTL RGCVESY
Sbjct: 206 SVSSYQRFVIQLLMDFLQGLWEEGGRRIAVVGLPPIGCLPIVITLNLDKTTLPRGCVESY 265

Query: 126 SAAARGFNQILKKELKSMQSKLAKDNVKFYYVDSYGPLADMIHNFHNYGFEEAGSGCCGS 185
           SAAARGFNQIL+ ELKSMQSKLAKDNVKF+YVDSY PLADMIHNFHNYGFEEAGSGCCGS
Sbjct: 266 SAAARGFNQILRMELKSMQSKLAKDNVKFFYVDSYEPLADMIHNFHNYGFEEAGSGCCGS 325

Query: 186 GYVEASFLCNPKTYTCPDASKYVFWDSIHPTQATYHNIFLAGRPIVDAMLKH 238
           GYVEASFLCNPKT TCPDASKYVFWDSIHPTQATYHNIFLAGRPIVDAMLKH
Sbjct: 326 GYVEASFLCNPKTDTCPDASKYVFWDSIHPTQATYHNIFLAGRPIVDAMLKH 377

BLAST of Carg15326 vs. ExPASy TrEMBL
Match: A0A1S3B072 (GDSL esterase/lipase At5g45960 OS=Cucumis melo OX=3656 GN=LOC103484476 PE=3 SV=1)

HSP 1 Score: 362.8 bits (930), Expect = 1.1e-96
Identity = 169/230 (73.48%), Postives = 198/230 (86.09%), Query Frame = 0

Query: 6   NVIPLRKQLDYLKEYKKRVELGMGKDRAEAHIKKAAFVVSAGTNDFVLNYFLLPFRRQIY 65
           NVI +  QL+ LKEYKK++E G+GK+R EAH+KKA +VVSAGTNDFV+NYFLLPFRR+ Y
Sbjct: 145 NVISMSSQLELLKEYKKQIESGIGKNRTEAHMKKAVYVVSAGTNDFVVNYFLLPFRRKTY 204

Query: 66  SVSSYQRFVIQLLMDFLQGLWEEGGRRIAVVGLPPIGCLPIVITLNLDKTTLTRGCVESY 125
           S+SSYQ F+IQLL+ FLQGLW EGGR+IAVVGLPP+GCLP VITLN D T + RGCVE+Y
Sbjct: 205 SLSSYQHFIIQLLLHFLQGLWVEGGRKIAVVGLPPMGCLPAVITLNSDDTLVRRGCVEAY 264

Query: 126 SAAARGFNQILKKELKSMQSKLAKDNVKFYYVDSYGPLADMIHNFHNYGFEEAGSGCCGS 185
           S+AAR FNQIL+KEL+SMQSKLA+   KFYYVDSY PL+DMI   H YGFEE G+GCCG+
Sbjct: 265 SSAARTFNQILQKELQSMQSKLAESGAKFYYVDSYAPLSDMIAGSHKYGFEEVGNGCCGN 324

Query: 186 GYVEASFLCNPKTYTCPDASKYVFWDSIHPTQATYHNIFLAGRPIVDAML 236
           GYVEA FLCN KT TCPDASKYVFWDSIHPTQ  Y+N+FLA RP+VDA++
Sbjct: 325 GYVEAGFLCNTKTETCPDASKYVFWDSIHPTQKAYYNLFLAARPLVDAII 374

BLAST of Carg15326 vs. ExPASy TrEMBL
Match: A0A0A0KNH3 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_6G518310 PE=3 SV=1)

HSP 1 Score: 362.5 bits (929), Expect = 1.4e-96
Identity = 172/231 (74.46%), Postives = 200/231 (86.58%), Query Frame = 0

Query: 6   NVIPLRKQLDYLKEYKKRVELGMGKDRAEAHIKKAAFVVSAGTNDFVLNYFLLPFRRQIY 65
           NVI +  QL+ LKEYKKRVE G+GK+R EAH+KKA +V+SAGTNDFV+NYFLLPFRR+ Y
Sbjct: 143 NVISMSSQLELLKEYKKRVESGIGKNRTEAHMKKAVYVISAGTNDFVVNYFLLPFRRKSY 202

Query: 66  SVSSYQRFVIQLLMDFLQGLWEEGGRRIAVVGLPPIGCLPIVITLNLDKTTL-TRGCVES 125
           +VSSYQ F++QLL+ FLQGLW EGGR+IAVVGLPP+GCLP VITLN D T +  RGCVE+
Sbjct: 203 TVSSYQHFILQLLIHFLQGLWAEGGRKIAVVGLPPMGCLPAVITLNSDDTLVRRRGCVEA 262

Query: 126 YSAAARGFNQILKKELKSMQSKLAKDNVKFYYVDSYGPLADMIHNFHNYGFEEAGSGCCG 185
           YS+AAR FNQIL+KEL+SMQSKLA+   KFYYVDSYGPL+DMI  F+ YGFEE G+GCCG
Sbjct: 263 YSSAARTFNQILQKELQSMQSKLAESGAKFYYVDSYGPLSDMIAGFNKYGFEEVGNGCCG 322

Query: 186 SGYVEASFLCNPKTYTCPDASKYVFWDSIHPTQATYHNIFLAGRPIVDAML 236
           SGYVEA FLCN KT TCPDASKYVFWDSIHPTQ  Y+N+FLA RPIVDA++
Sbjct: 323 SGYVEAGFLCNTKTETCPDASKYVFWDSIHPTQKAYYNLFLATRPIVDAVI 373

BLAST of Carg15326 vs. ExPASy TrEMBL
Match: A0A059CD57 (Uncharacterized protein OS=Eucalyptus grandis OX=71139 GN=EUGRSUZ_D00575 PE=3 SV=1)

HSP 1 Score: 290.4 bits (742), Expect = 6.7e-75
Identity = 131/231 (56.71%), Postives = 176/231 (76.19%), Query Frame = 0

Query: 6   NVIPLRKQLDYLKEYKKRVELGMGKDRAEAHIKKAAFVVSAGTNDFVLNYFLLPFRRQIY 65
           NV+ L +QL+Y KEYKKR+   MG      HI KA F+VSAGTNDFV+NYF +P R++ +
Sbjct: 139 NVVSLPQQLEYFKEYKKRLMSTMGNQSTIQHINKAVFLVSAGTNDFVVNYFTIPIRQRSF 198

Query: 66  SVSSYQRFVIQLLMDFLQGLWEEGGRRIAVVGLPPIGCLPIVITLNLDKTTLTRGCVESY 125
           ++++YQ+F++Q + D ++GLWEEG RRIAV GLPP+GCLPI+I+LN +   L RGC++ Y
Sbjct: 199 TIATYQQFLLQHVKDLIKGLWEEGARRIAVAGLPPMGCLPIMISLNSNSAVLQRGCMDQY 258

Query: 126 SAAARGFNQILKKELKSMQSKLAKDNVKFYYVDSYGPLADMIHNFHNYGFEEAGSGCCGS 185
           S+ AR +N +L+ EL SMQS LA    + YY D Y P++DMI  F +YGF+EA SGCCG+
Sbjct: 259 SSVARDYNALLQTELASMQSSLANQGSRIYYADIYKPISDMILRFPSYGFDEALSGCCGT 318

Query: 186 GYVEASFLCNPKTYTCPDASKYVFWDSIHPTQATYHNIFLAGRPIVDAMLK 237
           G +EA+FLCNP +Y CPDASKYVFWDSIHPT+  Y+ IF   RPIV+A+++
Sbjct: 319 GLLEAAFLCNPNSYVCPDASKYVFWDSIHPTEKAYYLIFQLLRPIVNALIQ 369

BLAST of Carg15326 vs. TAIR 10
Match: AT5G45960.1 (GDSL-like Lipase/Acylhydrolase superfamily protein )

HSP 1 Score: 265.0 bits (676), Expect = 5.8e-71
Identity = 122/230 (53.04%), Postives = 167/230 (72.61%), Query Frame = 0

Query: 6   NVIPLRKQLDYLKEYKKRVELGMGKDRAEAHIKKAAFVVSAGTNDFVLNYFLLPFRRQIY 65
           NVI +  QL+Y +EYK+++E  MGK   E HI++A F VSAGTNDFV+NYF +P RR+ +
Sbjct: 143 NVIDIPTQLEYFREYKRKLEGKMGKQEMEKHIEEAMFCVSAGTNDFVINYFTIPIRRKTF 202

Query: 66  SVSSYQRFVIQLLMDFLQGLWEEGGRRIAVVGLPPIGCLPIVITLNLDKTTLTRGCVESY 125
           ++ +YQ+FVI  L  F+QGLW+EG R+I V GLPPIGCLPIVITL   +    R C++ +
Sbjct: 203 TIEAYQQFVISNLKQFIQGLWKEGARKITVAGLPPIGCLPIVITLFSGEALTNRRCIDRF 262

Query: 126 SAAARGFNQILKKELKSMQSKLAKDNVKFYYVDSYGPLADMIHNFHNYGFEEAGSGCCGS 185
           S  A  +N +L+K+L  MQ  LA    K +Y+D Y P+ ++I +   +GFEE  SGCCGS
Sbjct: 263 STVATNYNFLLQKQLALMQVGLAHLGSKIFYLDVYNPVYEVIRDPRKFGFEEVFSGCCGS 322

Query: 186 GYVEASFLCNPKTYTCPDASKYVFWDSIHPTQATYHNIFLAGRPIVDAML 236
           GY+EASFLCNPK+Y CP+ S YVF+DSIHP++ TY ++F + RPI D++L
Sbjct: 323 GYLEASFLCNPKSYVCPNTSAYVFFDSIHPSEKTYFSLFRSLRPIYDSIL 372

BLAST of Carg15326 vs. TAIR 10
Match: AT1G06990.1 (GDSL-like Lipase/Acylhydrolase superfamily protein )

HSP 1 Score: 179.9 bits (455), Expect = 2.4e-45
Identity = 90/221 (40.72%), Postives = 137/221 (61.99%), Query Frame = 0

Query: 6   NVIPLRKQLDYLKEYKKRVELGMGKDRAEAHIKKAAFVVSAGTNDFVLNYFLLPFRRQIY 65
           + + + KQ D L+ Y +R+   +G ++A + + +A  +VS+GTNDF LN +  P RRQ  
Sbjct: 133 STLSVDKQADMLRSYVERLSQIVGDEKAASIVSEALVIVSSGTNDFNLNLYDTPSRRQKL 192

Query: 66  SVSSYQRFVIQLLMDFLQGLWEEGGRRIAVVGLPPIGCLPIVITLNLDKTTLTRGCVESY 125
            V  YQ F++  + +F+Q L++ G R+I V+GLPP+GCLPI +T+ + K    R C++  
Sbjct: 193 GVDGYQSFILSNVHNFVQELYDIGCRKIMVLGLPPVGCLPIQMTMAMQKQN-ERRCIDKQ 252

Query: 126 SAAARGFNQILKKELKSMQSKLAKDNVKFYYVDSYGPLADMIHNFHNYGFEEAGSGCCGS 185
           ++ ++ FNQ LK  L  MQS L    +  +Y D YG L DM  N   YG +E   GCCG+
Sbjct: 253 NSDSQEFNQKLKNSLTEMQSNLTGSVI--FYGDIYGALFDMATNPQRYGLKETTRGCCGT 312

Query: 186 GYVEASFLCNPKTYTCPDASKYVFWDSIHPTQATYHNIFLA 227
           G +E ++LCN  T  CP+ ++Y+FWD IHP+Q  Y  I L+
Sbjct: 313 GEIELAYLCNALTRICPNPNQYLFWDDIHPSQIAYIVISLS 350

BLAST of Carg15326 vs. TAIR 10
Match: AT2G30310.1 (GDSL-like Lipase/Acylhydrolase family protein )

HSP 1 Score: 178.3 bits (451), Expect = 7.1e-45
Identity = 94/217 (43.32%), Postives = 135/217 (62.21%), Query Frame = 0

Query: 8   IPLRKQLDYLKEYKKRVELGMGKDRAEAHIKKAAFVVSAGTNDFVLNYFLLPFRRQIY-S 67
           IP+ +Q    K Y  R++  +G  +A   I  A  V+SAG NDF+LN++ +P RR  Y +
Sbjct: 133 IPVSQQPSMFKNYIARLKGIVGDKKAMEIINNALVVISAGPNDFILNFYDIPTRRLEYPT 192

Query: 68  VSSYQRFVIQLLMDFLQGLWEEGGRRIAVVGLPPIGCLPIVITLNLDKTTLTRGCVESYS 127
           +  YQ F+++ L  F++ L+  G R I V GLPP+GCLPI +T  +    + R CVE  +
Sbjct: 193 IHGYQEFILKRLDGFVRELYSLGCRNIVVGGLPPMGCLPIQMTAKM--RNILRFCVEQEN 252

Query: 128 AAARGFNQILKKELKSMQSKLAKDNVKFYYVDSYGPLADMIHNFHNYGFEEAGSGCCGSG 187
             +  +NQ L K+L  +Q+ L   N  F Y + Y PL DMI N   YGF+E   GCCG+G
Sbjct: 253 KDSVLYNQKLVKKLPEIQASLPGSN--FLYANVYDPLMDMIQNPSKYGFKETKKGCCGTG 312

Query: 188 YVEASFLCNPKTYTCPDASKYVFWDSIHPTQATYHNI 224
           Y+E +F+CNP T TCP+ S ++FWDSIHP++A Y+ I
Sbjct: 313 YLETTFMCNPLTKTCPNHSDHLFWDSIHPSEAAYNYI 345

BLAST of Carg15326 vs. TAIR 10
Match: AT4G26790.1 (GDSL-like Lipase/Acylhydrolase superfamily protein )

HSP 1 Score: 171.8 bits (434), Expect = 6.7e-43
Identity = 85/215 (39.53%), Postives = 136/215 (63.26%), Query Frame = 0

Query: 6   NVIPLRKQLDYLKEYKKRVELGMGKDRAEAHIKKAAFVVSAGTNDFVLNYFLLPFRRQIY 65
           +V+PL K+++Y KEY+ R+   +G+++A   I ++ +++S GTNDF+ NY+LLP + + Y
Sbjct: 125 SVMPLWKEVEYYKEYQTRLRSYLGEEKANEIISESLYLISIGTNDFLENYYLLPRKLRKY 184

Query: 66  SVSSYQRFVIQLLMDFLQGLWEEGGRRIAVVGLPPIGCLPIVITLNLDKTTLTRGCVESY 125
           SV+ YQ F+I +  DF+  ++  G R++++ GL P GCLP+  T  L        C+E Y
Sbjct: 185 SVNEYQYFLIGIAADFVTDIYRLGARKMSLSGLSPFGCLPLERTTQL---FYGSKCIEEY 244

Query: 126 SAAARGFNQILKKELKSMQSKLAKDNVKFYYVDSYGPLADMIHNFHNYGFEEAGSGCCGS 185
           +  AR FN  +K E K  Q     + ++  + + Y  ++++I++   +GFE   S CCG+
Sbjct: 245 NIVARDFN--IKMEEKVFQLNRDLNGIQLVFSNPYDLVSEIIYHPEAFGFENVRSACCGT 304

Query: 186 GYVEASFLCNP-KTYTCPDASKYVFWDSIHPTQAT 220
           GY E S+LC+    +TC DASKYVFWDS HPT+ T
Sbjct: 305 GYYEMSYLCDKMNPFTCSDASKYVFWDSFHPTEKT 334

BLAST of Carg15326 vs. TAIR 10
Match: AT4G26790.2 (GDSL-like Lipase/Acylhydrolase superfamily protein )

HSP 1 Score: 171.8 bits (434), Expect = 6.7e-43
Identity = 85/215 (39.53%), Postives = 136/215 (63.26%), Query Frame = 0

Query: 6   NVIPLRKQLDYLKEYKKRVELGMGKDRAEAHIKKAAFVVSAGTNDFVLNYFLLPFRRQIY 65
           +V+PL K+++Y KEY+ R+   +G+++A   I ++ +++S GTNDF+ NY+LLP + + Y
Sbjct: 125 SVMPLWKEVEYYKEYQTRLRSYLGEEKANEIISESLYLISIGTNDFLENYYLLPRKLRKY 184

Query: 66  SVSSYQRFVIQLLMDFLQGLWEEGGRRIAVVGLPPIGCLPIVITLNLDKTTLTRGCVESY 125
           SV+ YQ F+I +  DF+  ++  G R++++ GL P GCLP+  T  L        C+E Y
Sbjct: 185 SVNEYQYFLIGIAADFVTDIYRLGARKMSLSGLSPFGCLPLERTTQL---FYGSKCIEEY 244

Query: 126 SAAARGFNQILKKELKSMQSKLAKDNVKFYYVDSYGPLADMIHNFHNYGFEEAGSGCCGS 185
           +  AR FN  +K E K  Q     + ++  + + Y  ++++I++   +GFE   S CCG+
Sbjct: 245 NIVARDFN--IKMEEKVFQLNRDLNGIQLVFSNPYDLVSEIIYHPEAFGFENVRSACCGT 304

Query: 186 GYVEASFLCNP-KTYTCPDASKYVFWDSIHPTQAT 220
           GY E S+LC+    +TC DASKYVFWDS HPT+ T
Sbjct: 305 GYYEMSYLCDKMNPFTCSDASKYVFWDSFHPTEKT 334