Carg15240 (gene) Silver-seed gourd (SMH-JMG-627) v2

Overview
NameCarg15240
Typegene
OrganismCucurbita argyrosperma subsp. argyrosperma cv. SMH-JMG-627 (Silver-seed gourd (SMH-JMG-627) v2)
DescriptionOxysterol-binding protein-related protein 4B-like
LocationCarg_Chr10: 1860071 .. 1860582 (-)
RNA-Seq ExpressionCarg15240
SyntenyCarg15240
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideexonCDS
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGAAGGAGGAGACAAACGGCGGATTTGTTCTGCCATTGGATAGTTTTCCGACGAGAAACTCATCTATGGGTCGTTCCTCTCGTTCGTACTACTACAGAAGGACTTCTGAAGGGGTTCCGTTTCAGTGGGAGATGCTACCGGGGACCCCCAAGAATACTCCGCCGCTCGTGGAGCCGGTTCCAGTGCGCATCCGCCCGCCGCCGGTTGTGGTCAGCCTTAGCCTCTTGAAGCCGGCCACGGTTCAGAATAAACAGCCGCAGTTTCTGTTATTTCGGCCGTGGAGGAAGCAGTCCAAGTCCAACAACAATTCTCGTCCGTTGGATGACCATCGTTCGAAATTTGGATCTCCATGCAAAGCAAAATCTCCTACGCCGGCGGCGGAATCTCCCGGGCACTGGCGGTTTGGATGCCGTCCGTGGAGGATCAACCCTATTAAAATAGGTACTGGAAGGCGAGTTTAATTTCGTGTATTATCGATATCTTTTTTTTGGTAGATTTTGTAAATAAATAA

mRNA sequence

ATGAAGGAGGAGACAAACGGCGGATTTGTTCTGCCATTGGATAGTTTTCCGACGAGAAACTCATCTATGGGTCGTTCCTCTCGTTCGTACTACTACAGAAGGACTTCTGAAGGGGTTCCGTTTCAGTGGGAGATGCTACCGGGGACCCCCAAGAATACTCCGCCGCTCGTGGAGCCGGTTCCAGTGCGCATCCGCCCGCCGCCGGTTGTGGTCAGCCTTAGCCTCTTGAAGCCGGCCACGGTTCAGAATAAACAGCCGCAGTTTCTGTTATTTCGGCCGTGGAGGAAGCAGTCCAAGTCCAACAACAATTCTCGTCCGTTGGATGACCATCGTTCGAAATTTGGATCTCCATGCAAAGCAAAATCTCCTACGCCGGCGGCGGAATCTCCCGGGCACTGGCGGTTTGGATGCCGTCCGTGGAGGATCAACCCTATTAAAATAGATTTTGTAAATAAATAA

Coding sequence (CDS)

ATGAAGGAGGAGACAAACGGCGGATTTGTTCTGCCATTGGATAGTTTTCCGACGAGAAACTCATCTATGGGTCGTTCCTCTCGTTCGTACTACTACAGAAGGACTTCTGAAGGGGTTCCGTTTCAGTGGGAGATGCTACCGGGGACCCCCAAGAATACTCCGCCGCTCGTGGAGCCGGTTCCAGTGCGCATCCGCCCGCCGCCGGTTGTGGTCAGCCTTAGCCTCTTGAAGCCGGCCACGGTTCAGAATAAACAGCCGCAGTTTCTGTTATTTCGGCCGTGGAGGAAGCAGTCCAAGTCCAACAACAATTCTCGTCCGTTGGATGACCATCGTTCGAAATTTGGATCTCCATGCAAAGCAAAATCTCCTACGCCGGCGGCGGAATCTCCCGGGCACTGGCGGTTTGGATGCCGTCCGTGGAGGATCAACCCTATTAAAATAGATTTTGTAAATAAATAA

Protein sequence

MKEETNGGFVLPLDSFPTRNSSMGRSSRSYYYRRTSEGVPFQWEMLPGTPKNTPPLVEPVPVRIRPPPVVVSLSLLKPATVQNKQPQFLLFRPWRKQSKSNNNSRPLDDHRSKFGSPCKAKSPTPAAESPGHWRFGCRPWRINPIKIDFVNK
Homology
BLAST of Carg15240 vs. NCBI nr
Match: KAG7023386.1 (hypothetical protein SDJN02_14411, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 319.3 bits (817), Expect = 1.8e-83
Identity = 152/152 (100.00%), Postives = 152/152 (100.00%), Query Frame = 0

Query: 1   MKEETNGGFVLPLDSFPTRNSSMGRSSRSYYYRRTSEGVPFQWEMLPGTPKNTPPLVEPV 60
           MKEETNGGFVLPLDSFPTRNSSMGRSSRSYYYRRTSEGVPFQWEMLPGTPKNTPPLVEPV
Sbjct: 1   MKEETNGGFVLPLDSFPTRNSSMGRSSRSYYYRRTSEGVPFQWEMLPGTPKNTPPLVEPV 60

Query: 61  PVRIRPPPVVVSLSLLKPATVQNKQPQFLLFRPWRKQSKSNNNSRPLDDHRSKFGSPCKA 120
           PVRIRPPPVVVSLSLLKPATVQNKQPQFLLFRPWRKQSKSNNNSRPLDDHRSKFGSPCKA
Sbjct: 61  PVRIRPPPVVVSLSLLKPATVQNKQPQFLLFRPWRKQSKSNNNSRPLDDHRSKFGSPCKA 120

Query: 121 KSPTPAAESPGHWRFGCRPWRINPIKIDFVNK 153
           KSPTPAAESPGHWRFGCRPWRINPIKIDFVNK
Sbjct: 121 KSPTPAAESPGHWRFGCRPWRINPIKIDFVNK 152

BLAST of Carg15240 vs. NCBI nr
Match: KAG6589706.1 (hypothetical protein SDJN03_15129, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 261.2 bits (666), Expect = 5.7e-66
Identity = 124/125 (99.20%), Postives = 124/125 (99.20%), Query Frame = 0

Query: 23  MGRSSRSYYYRRTSEGVPFQWEMLPGTPKNTPPLVEPVPVRIRPPPVVVSLSLLKPATVQ 82
           MGRSSRSYYYRRTSEGVPFQWEMLPGTPKNTPPLVEPVPVRIRPPPVVVSLSLLKPATVQ
Sbjct: 1   MGRSSRSYYYRRTSEGVPFQWEMLPGTPKNTPPLVEPVPVRIRPPPVVVSLSLLKPATVQ 60

Query: 83  NKQPQFLLFRPWRKQSKSNNNSRPLDDHRSKFGSPCKAKSPTPAAESPGHWRFGCRPWRI 142
           NKQPQFLLFRPWRKQSKSNNNSRPLDDHRSKFGSPCKAKSPTPAAESPGHWRFGC PWRI
Sbjct: 61  NKQPQFLLFRPWRKQSKSNNNSRPLDDHRSKFGSPCKAKSPTPAAESPGHWRFGCSPWRI 120

Query: 143 NPIKI 148
           NPIKI
Sbjct: 121 NPIKI 125

BLAST of Carg15240 vs. NCBI nr
Match: XP_004146363.1 (uncharacterized protein LOC101210202 [Cucumis sativus] >KGN65458.1 hypothetical protein Csa_019691 [Cucumis sativus])

HSP 1 Score: 170.6 bits (431), Expect = 1.0e-38
Identity = 100/167 (59.88%), Postives = 109/167 (65.27%), Query Frame = 0

Query: 1   MKEETNGG--FVLPLDSFPTRNSSMGRSSRSYYYRRTSEGVPFQWEMLPGTPKNTPPLVE 60
           MKEE N    FVLPLD+FP+RNSSM RSSR +YYRRT+EGVPFQWEM PGTPKN PPL E
Sbjct: 1   MKEEKNSNELFVLPLDNFPSRNSSMSRSSR-FYYRRTTEGVPFQWEMQPGTPKNNPPLTE 60

Query: 61  PVPVRIRPPPVVVSLSLLKPA-TVQNKQPQFLLFRPWRKQ--SKSNNNSRPLD----DHR 120
            +   I PPP V+SL L KP   VQNKQP F+LF PWRKQ   K   N  PLD    + R
Sbjct: 61  VILPPISPPPAVLSLGLSKPVRAVQNKQPLFVLFGPWRKQPKPKPKKNPCPLDLNNKNER 120

Query: 121 SKFG-----------SPCKAKSPTPAAESPGHWRFGCRPWRINPIKI 148
            +F            S CK KS  PA  S GHWR GC PWRINPIKI
Sbjct: 121 LRFDSCGSQCELMAESSCKGKS-APAGGSSGHWRLGCGPWRINPIKI 165

BLAST of Carg15240 vs. NCBI nr
Match: XP_023530969.1 (uncharacterized protein LOC111793357 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 156.4 bits (394), Expect = 2.0e-34
Identity = 90/166 (54.22%), Postives = 106/166 (63.86%), Query Frame = 0

Query: 3   EETNGGFVLPLDSFPTRNSSMGRSSRSYYYRRTSEGVPFQWEMLPGTPKNTPPLVEPVPV 62
           E+ +  FVLPLD+ P+R SS+G SSR YYYRRT+EGVPFQWEM PGTPK+  P  E V +
Sbjct: 4   EKDDAEFVLPLDNLPSRCSSLGCSSRLYYYRRTTEGVPFQWEMQPGTPKD-QPYTEAVTL 63

Query: 63  RIRPPPVVVSLSLLKPATVQNKQPQFLLFRPWRKQSKSNNN---SRPLDDHRSKFG---- 122
            I PPP  +SL   KPA  Q KQ  FLLFRPWRKQSKS  +   S   +D RS+FG    
Sbjct: 64  LITPPPAALSLRPPKPARTQGKQSLFLLFRPWRKQSKSKKHSCLSDEFEDGRSRFGSCGS 123

Query: 123 ------SPCKAKSPT--------PAAESPGHWRFGCRPWRINPIKI 148
                 SPC+ KS +        PAAES  H R GC PWRINP++I
Sbjct: 124 QCEFIQSPCQPKSSSSSSSWSSLPAAESSAHRRLGCGPWRINPLRI 168

BLAST of Carg15240 vs. NCBI nr
Match: XP_023007086.1 (uncharacterized protein LOC111499680 [Cucurbita maxima])

HSP 1 Score: 153.7 bits (387), Expect = 1.3e-33
Identity = 88/165 (53.33%), Postives = 105/165 (63.64%), Query Frame = 0

Query: 3   EETNGGFVLPLDSFPTRNSSMGRSSRSYYYRRTSEGVPFQWEMLPGTPKNTPPLVEPVPV 62
           E+ +  FVL LD+ P+R SS+G SSR YYYRRT+EGVPFQWEM PGTPK+ PP  E V +
Sbjct: 4   EKDDAEFVLSLDNLPSRCSSLGCSSRLYYYRRTTEGVPFQWEMQPGTPKD-PPYTEAVTL 63

Query: 63  RIRPPPVVVSLSLLKPATVQNKQPQFLLFRPWRKQSKSNNN---SRPLDDHRSKFG---- 122
            I PPP  +SL   KPA    KQ  FLLFRPWRKQSKS  +   S   +D R++FG    
Sbjct: 64  LITPPPAALSLRPPKPARTHGKQSLFLLFRPWRKQSKSKKHSCLSDEFEDGRTRFGSCGS 123

Query: 123 ------SPCKAKSPT-------PAAESPGHWRFGCRPWRINPIKI 148
                 SPC+ KS +       PAAES  H R GC PWRINP++I
Sbjct: 124 QCEFIQSPCEPKSSSSSAWSSLPAAESSAHRRLGCGPWRINPLRI 167

BLAST of Carg15240 vs. ExPASy TrEMBL
Match: A0A0A0LUL8 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_1G423160 PE=4 SV=1)

HSP 1 Score: 170.6 bits (431), Expect = 4.9e-39
Identity = 100/167 (59.88%), Postives = 109/167 (65.27%), Query Frame = 0

Query: 1   MKEETNGG--FVLPLDSFPTRNSSMGRSSRSYYYRRTSEGVPFQWEMLPGTPKNTPPLVE 60
           MKEE N    FVLPLD+FP+RNSSM RSSR +YYRRT+EGVPFQWEM PGTPKN PPL E
Sbjct: 1   MKEEKNSNELFVLPLDNFPSRNSSMSRSSR-FYYRRTTEGVPFQWEMQPGTPKNNPPLTE 60

Query: 61  PVPVRIRPPPVVVSLSLLKPA-TVQNKQPQFLLFRPWRKQ--SKSNNNSRPLD----DHR 120
            +   I PPP V+SL L KP   VQNKQP F+LF PWRKQ   K   N  PLD    + R
Sbjct: 61  VILPPISPPPAVLSLGLSKPVRAVQNKQPLFVLFGPWRKQPKPKPKKNPCPLDLNNKNER 120

Query: 121 SKFG-----------SPCKAKSPTPAAESPGHWRFGCRPWRINPIKI 148
            +F            S CK KS  PA  S GHWR GC PWRINPIKI
Sbjct: 121 LRFDSCGSQCELMAESSCKGKS-APAGGSSGHWRLGCGPWRINPIKI 165

BLAST of Carg15240 vs. ExPASy TrEMBL
Match: A0A6J1KZJ1 (uncharacterized protein LOC111499680 OS=Cucurbita maxima OX=3661 GN=LOC111499680 PE=4 SV=1)

HSP 1 Score: 153.7 bits (387), Expect = 6.3e-34
Identity = 88/165 (53.33%), Postives = 105/165 (63.64%), Query Frame = 0

Query: 3   EETNGGFVLPLDSFPTRNSSMGRSSRSYYYRRTSEGVPFQWEMLPGTPKNTPPLVEPVPV 62
           E+ +  FVL LD+ P+R SS+G SSR YYYRRT+EGVPFQWEM PGTPK+ PP  E V +
Sbjct: 4   EKDDAEFVLSLDNLPSRCSSLGCSSRLYYYRRTTEGVPFQWEMQPGTPKD-PPYTEAVTL 63

Query: 63  RIRPPPVVVSLSLLKPATVQNKQPQFLLFRPWRKQSKSNNN---SRPLDDHRSKFG---- 122
            I PPP  +SL   KPA    KQ  FLLFRPWRKQSKS  +   S   +D R++FG    
Sbjct: 64  LITPPPAALSLRPPKPARTHGKQSLFLLFRPWRKQSKSKKHSCLSDEFEDGRTRFGSCGS 123

Query: 123 ------SPCKAKSPT-------PAAESPGHWRFGCRPWRINPIKI 148
                 SPC+ KS +       PAAES  H R GC PWRINP++I
Sbjct: 124 QCEFIQSPCEPKSSSSSAWSSLPAAESSAHRRLGCGPWRINPLRI 167

BLAST of Carg15240 vs. ExPASy TrEMBL
Match: A0A6J1EQV3 (uncharacterized protein LOC111436686 OS=Cucurbita moschata OX=3662 GN=LOC111436686 PE=4 SV=1)

HSP 1 Score: 153.3 bits (386), Expect = 8.2e-34
Identity = 89/165 (53.94%), Postives = 105/165 (63.64%), Query Frame = 0

Query: 3   EETNGGFVLPLDSFPTRNSSMGRSSRSYYYRRTSEGVPFQWEMLPGTPKNTPPLVEPVPV 62
           E+ +  FVL LD+ P+R SS+G SSR YYYRRT+EGVPFQWEM PGTPK+ PP  E V +
Sbjct: 4   EKDDAEFVLSLDNLPSRCSSLGCSSRLYYYRRTTEGVPFQWEMQPGTPKD-PPYTEAVTL 63

Query: 63  RIRPPPVVVSLSLLKPATVQNKQPQFLLFRPWRKQSKSNNNSRPLD---DHRSKFG---- 122
            I PPP  +SL   KPA    KQ  FLLFRPWRKQSKS  +S   D   D R++FG    
Sbjct: 64  LITPPPAALSLRPPKPARTHGKQSLFLLFRPWRKQSKSQKHSCLSDEFGDGRTRFGSCGS 123

Query: 123 ------SPCKAKSPT-------PAAESPGHWRFGCRPWRINPIKI 148
                 SPC+ KS +       PAAES  H R GC PWRINP++I
Sbjct: 124 QCEFIQSPCEPKSSSSSSWSSLPAAESSAHRRLGCGPWRINPLRI 167

BLAST of Carg15240 vs. ExPASy TrEMBL
Match: A0A5D3CJV8 (Oxysterol-binding protein-related protein 4B-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold152G00380 PE=4 SV=1)

HSP 1 Score: 133.3 bits (334), Expect = 8.7e-28
Identity = 79/145 (54.48%), Postives = 89/145 (61.38%), Query Frame = 0

Query: 23  MGRSSRSYYYRRTSEGVPFQWEMLPGTPKNTPPLVEPVPVRIRPPPVVVSLSLLKPA-TV 82
           M RSSR +YYRRT+EGVPFQWEM PGTPK+ PPL E +P  I PPP V+SL   KP   V
Sbjct: 1   MSRSSR-FYYRRTTEGVPFQWEMQPGTPKSNPPLTEVIPPPISPPPAVLSLGFSKPVRVV 60

Query: 83  QNKQPQFLLFRPWRKQ----SKSNNNSRPLD----DHRSKFG-----------SPCKAKS 142
           QNKQ  F+LF PWRKQ     K   N  PLD    + R +F            S CK +S
Sbjct: 61  QNKQSLFVLFGPWRKQPKPKPKPKKNPCPLDLNKNNERLRFDSCGSQCELMAESSCKDRS 120

Query: 143 PTPAAESPGHWRFGCRPWRINPIKI 148
            +P  ES G WR GC PWRINPIKI
Sbjct: 121 -SPVGESSGQWRLGCGPWRINPIKI 143

BLAST of Carg15240 vs. ExPASy TrEMBL
Match: A0A7J9CW56 (Uncharacterized protein OS=Gossypium gossypioides OX=34282 GN=Gogos_001480 PE=4 SV=1)

HSP 1 Score: 81.6 bits (200), Expect = 3.0e-12
Identity = 58/151 (38.41%), Postives = 79/151 (52.32%), Query Frame = 0

Query: 18  TRNSSMGRSSRSYYYRRTSEGVPFQWEMLPGTPKNTPPLVEPVPVRIRPPPVVVSLSLLK 77
           +R+SS+G SSR YYY R+SEG+PF WEM PGTPK   P  E V   I PPP ++SL L K
Sbjct: 24  SRDSSVGCSSRIYYY-RSSEGIPFNWEMQPGTPKE--PRKEDVLPPISPPPALLSLGLPK 83

Query: 78  P---ATVQNKQPQFLLFRPWRKQSKSNNNSRPLDDHRSK----------------FGSPC 137
           P      +NK  +  +F+ W K  + N+N + +DDH+S                  GSPC
Sbjct: 84  PRIDVVEENKVSKLKVFKFW-KHGRKNDNKKKIDDHQSDKYSNYETCSSDDDGEFVGSPC 143

Query: 138 KA----------KSPTPAAESPGHWRFGCRP 140
            +           S TP+ +S  +  +GC P
Sbjct: 144 VSSSSSSSFSFGSSSTPSRDSSFNRHYGCGP 170

BLAST of Carg15240 vs. TAIR 10
Match: AT4G25845.1 (BEST Arabidopsis thaliana protein match is: OSBP(oxysterol binding protein)-related protein 4B (TAIR:AT4G25850.2); Has 35333 Blast hits to 34131 proteins in 2444 species: Archae - 798; Bacteria - 22429; Metazoa - 974; Fungi - 991; Plants - 531; Viruses - 0; Other Eukaryotes - 9610 (source: NCBI BLink). )

HSP 1 Score: 56.2 bits (134), Expect = 2.6e-08
Identity = 36/74 (48.65%), Postives = 48/74 (64.86%), Query Frame = 0

Query: 18 TRNSSMGRSSRSYYYRRTSEGVPFQWEMLPGTPKNTPPLVEPVPVRIRPPPVVVSLSLLK 77
          +RNSS+G     YY  R  EGVPF+WEM PGTP NT P  E VP  + PPP ++SL L K
Sbjct: 13 SRNSSVG-----YYDERMPEGVPFEWEMQPGTPINTQPR-EVVP-PLSPPPAMLSLGLPK 72

Query: 78 PATVQNKQPQFLLF 92
          P ++  ++P+  +F
Sbjct: 73 P-SISIEEPKNFVF 78

BLAST of Carg15240 vs. TAIR 10
Match: AT4G25850.2 (OSBP(oxysterol binding protein)-related protein 4B )

HSP 1 Score: 56.2 bits (134), Expect = 2.6e-08
Identity = 36/74 (48.65%), Postives = 48/74 (64.86%), Query Frame = 0

Query: 18 TRNSSMGRSSRSYYYRRTSEGVPFQWEMLPGTPKNTPPLVEPVPVRIRPPPVVVSLSLLK 77
          +RNSS+G     YY  R  EGVPF+WEM PGTP NT P  E VP  + PPP ++SL L K
Sbjct: 13 SRNSSVG-----YYDERMPEGVPFEWEMQPGTPINTQPR-EVVP-PLSPPPAMLSLGLPK 72

Query: 78 PATVQNKQPQFLLF 92
          P ++  ++P+  +F
Sbjct: 73 P-SISIEEPKNFVF 78

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
KAG7023386.11.8e-83100.00hypothetical protein SDJN02_14411, partial [Cucurbita argyrosperma subsp. argyro... [more]
KAG6589706.15.7e-6699.20hypothetical protein SDJN03_15129, partial [Cucurbita argyrosperma subsp. sorori... [more]
XP_004146363.11.0e-3859.88uncharacterized protein LOC101210202 [Cucumis sativus] >KGN65458.1 hypothetical ... [more]
XP_023530969.12.0e-3454.22uncharacterized protein LOC111793357 [Cucurbita pepo subsp. pepo][more]
XP_023007086.11.3e-3353.33uncharacterized protein LOC111499680 [Cucurbita maxima][more]
Match NameE-valueIdentityDescription
Match NameE-valueIdentityDescription
A0A0A0LUL84.9e-3959.88Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_1G423160 PE=4 SV=1[more]
A0A6J1KZJ16.3e-3453.33uncharacterized protein LOC111499680 OS=Cucurbita maxima OX=3661 GN=LOC111499680... [more]
A0A6J1EQV38.2e-3453.94uncharacterized protein LOC111436686 OS=Cucurbita moschata OX=3662 GN=LOC1114366... [more]
A0A5D3CJV88.7e-2854.48Oxysterol-binding protein-related protein 4B-like OS=Cucumis melo var. makuwa OX... [more]
A0A7J9CW563.0e-1238.41Uncharacterized protein OS=Gossypium gossypioides OX=34282 GN=Gogos_001480 PE=4 ... [more]
Match NameE-valueIdentityDescription
AT4G25845.12.6e-0848.65BEST Arabidopsis thaliana protein match is: OSBP(oxysterol binding protein)-rela... [more]
AT4G25850.22.6e-0848.65OSBP(oxysterol binding protein)-related protein 4B [more]
InterPro
Analysis Name: InterPro Annotations of Silver-seed gourd (SMH-JMG-627) v2
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 1..27
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 102..116
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 90..130
NoneNo IPR availablePANTHERPTHR33257:SF6OXYSTEROL-BINDING 4B-LIKE PROTEIN-RELATEDcoord: 16..111
NoneNo IPR availablePANTHERPTHR33257OS05G0165500 PROTEINcoord: 16..111

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Carg15240-RACarg15240-RAmRNA