Carg15037 (gene) Silver-seed gourd (SMH-JMG-627) v2

BLAST of Carg15037 vs. NCBI nr
Match: KAG7014677.1 (Subtilisin-like protease SBT3.3 [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 1546.2 bits (4002), Expect = 0.0e+00
Identity = 773/773 (100.00%), Postives = 773/773 (100.00%), Query Frame = 0

Query: 1   MGSFAHGKSSSTTIFLVCISVFTALDHLCTLHIRVAAKSNVYIVYMGQKPHDNEELLVKA 60
           MGSFAHGKSSSTTIFLVCISVFTALDHLCTLHIRVAAKSNVYIVYMGQKPHDNEELLVKA
Sbjct: 1   MGSFAHGKSSSTTIFLVCISVFTALDHLCTLHIRVAAKSNVYIVYMGQKPHDNEELLVKA 60

Query: 61  HHGVLASVLGSQEGSVDSLVYSYKYGFSGFAAKLTMAQAQMISELPSVVEVIPNRLHKMQ 120
           HHGVLASVLGSQEGSVDSLVYSYKYGFSGFAAKLTMAQAQMISELPSVVEVIPNRLHKMQ
Sbjct: 61  HHGVLASVLGSQEGSVDSLVYSYKYGFSGFAAKLTMAQAQMISELPSVVEVIPNRLHKMQ 120

Query: 121 TTRSWDYLQLSPRFPNSLLRKSRMGSGAIIGVLDTGIWPESEVFCDEGLGPVPSRWKGIC 180
           TTRSWDYLQLSPRFPNSLLRKSRMGSGAIIGVLDTGIWPESEVFCDEGLGPVPSRWKGIC
Sbjct: 121 TTRSWDYLQLSPRFPNSLLRKSRMGSGAIIGVLDTGIWPESEVFCDEGLGPVPSRWKGIC 180

Query: 181 ESGELFSPAKACSRKLIGARYFVKGLEAAYGHPLNNSEFQDYWSPRDRSGHGTHVSSVAS 240
           ESGELFSPAKACSRKLIGARYFVKGLEAAYGHPLNNSEFQDYWSPRDRSGHGTHVSSVAS
Sbjct: 181 ESGELFSPAKACSRKLIGARYFVKGLEAAYGHPLNNSEFQDYWSPRDRSGHGTHVSSVAS 240

Query: 241 GFFVPNVSYHGLAVGTVRGGAPHSRIAMYKVCWQVNGGVCADIDILKAIDQAIYDGVDVL 300
           GFFVPNVSYHGLAVGTVRGGAPHSRIAMYKVCWQVNGGVCADIDILKAIDQAIYDGVDVL
Sbjct: 241 GFFVPNVSYHGLAVGTVRGGAPHSRIAMYKVCWQVNGGVCADIDILKAIDQAIYDGVDVL 300

Query: 301 SLSLGPSFPSYSDVDMRNGIAIGAFHAVAKGIVVVGAAGNSGPAAYSVSNIEPWLLTVAA 360
           SLSLGPSFPSYSDVDMRNGIAIGAFHAVAKGIVVVGAAGNSGPAAYSVSNIEPWLLTVAA
Sbjct: 301 SLSLGPSFPSYSDVDMRNGIAIGAFHAVAKGIVVVGAAGNSGPAAYSVSNIEPWLLTVAA 360

Query: 361 SSVDRSFLVAITLGNNWTTMGQGMFSGKLTKFHNLVYPEVSDLDDERACESLSLNDTWAA 420
           SSVDRSFLVAITLGNNWTTMGQGMFSGKLTKFHNLVYPEVSDLDDERACESLSLNDTWAA
Sbjct: 361 SSVDRSFLVAITLGNNWTTMGQGMFSGKLTKFHNLVYPEVSDLDDERACESLSLNDTWAA 420

Query: 421 GNVVLCFASDDYNDDTHNTSSSVKKVGGLGLIVAKNPTKAVEPFINNFPCVQISLDIGMQ 480
           GNVVLCFASDDYNDDTHNTSSSVKKVGGLGLIVAKNPTKAVEPFINNFPCVQISLDIGMQ
Sbjct: 421 GNVVLCFASDDYNDDTHNTSSSVKKVGGLGLIVAKNPTKAVEPFINNFPCVQISLDIGMQ 480

Query: 481 ILNYIRSTRNPQVKIGASTTRVGQPLSTTVAYFSSRGPNSVAPAILKPDIAAPGVAILAA 540
           ILNYIRSTRNPQVKIGASTTRVGQPLSTTVAYFSSRGPNSVAPAILKPDIAAPGVAILAA
Sbjct: 481 ILNYIRSTRNPQVKIGASTTRVGQPLSTTVAYFSSRGPNSVAPAILKPDIAAPGVAILAA 540

Query: 541 VPPSDPKDRNSYAFISGTSMATPHVSAIVALLKTLHSHWSPAAIKSAIVTTAWSSDPYGA 600
           VPPSDPKDRNSYAFISGTSMATPHVSAIVALLKTLHSHWSPAAIKSAIVTTAWSSDPYGA
Sbjct: 541 VPPSDPKDRNSYAFISGTSMATPHVSAIVALLKTLHSHWSPAAIKSAIVTTAWSSDPYGA 600

Query: 601 VIFAEGQPMMSARPFDFGGGIVNPNKAVDPGLVYDMGMADYIQYFCAKGYNDSAISGITK 660
           VIFAEGQPMMSARPFDFGGGIVNPNKAVDPGLVYDMGMADYIQYFCAKGYNDSAISGITK
Sbjct: 601 VIFAEGQPMMSARPFDFGGGIVNPNKAVDPGLVYDMGMADYIQYFCAKGYNDSAISGITK 660

Query: 661 RSISCPKRRPSILDINVPSITIPSLTHPVSLTRTVTNVGAINSSYKAAIEAPPGITIAIK 720
           RSISCPKRRPSILDINVPSITIPSLTHPVSLTRTVTNVGAINSSYKAAIEAPPGITIAIK
Sbjct: 661 RSISCPKRRPSILDINVPSITIPSLTHPVSLTRTVTNVGAINSSYKAAIEAPPGITIAIK 720

Query: 721 PRILKFNHKMKSISFTVTISSNHRVTTGYCFGSLTWLDGVHSVRIPVSVRTNI 774
           PRILKFNHKMKSISFTVTISSNHRVTTGYCFGSLTWLDGVHSVRIPVSVRTNI
Sbjct: 721 PRILKFNHKMKSISFTVTISSNHRVTTGYCFGSLTWLDGVHSVRIPVSVRTNI 773

BLAST of Carg15037 vs. NCBI nr
Match: XP_022922598.1 (subtilisin-like protease SBT3.9 isoform X1 [Cucurbita moschata])

HSP 1 Score: 1540.8 bits (3988), Expect = 0.0e+00
Identity = 770/773 (99.61%), Postives = 772/773 (99.87%), Query Frame = 0

Query: 1   MGSFAHGKSSSTTIFLVCISVFTALDHLCTLHIRVAAKSNVYIVYMGQKPHDNEELLVKA 60
           MGSFAHGKSSSTTIFLVCISVFTALDHLCTLHIRVAAKSNVYIVYMGQKPHDNEELLVKA
Sbjct: 1   MGSFAHGKSSSTTIFLVCISVFTALDHLCTLHIRVAAKSNVYIVYMGQKPHDNEELLVKA 60

Query: 61  HHGVLASVLGSQEGSVDSLVYSYKYGFSGFAAKLTMAQAQMISELPSVVEVIPNRLHKMQ 120
           HHGVLASVLGSQEGSVDSLVYSYKYGFSGFAAKLTMAQAQMISELPSVVEVIPNRLHKMQ
Sbjct: 61  HHGVLASVLGSQEGSVDSLVYSYKYGFSGFAAKLTMAQAQMISELPSVVEVIPNRLHKMQ 120

Query: 121 TTRSWDYLQLSPRFPNSLLRKSRMGSGAIIGVLDTGIWPESEVFCDEGLGPVPSRWKGIC 180
           TTRSWDYLQLSPRFPNSLLRKSRMGSGAIIGVLDTGIWPESEVFCDEGLGPVPSRWKGIC
Sbjct: 121 TTRSWDYLQLSPRFPNSLLRKSRMGSGAIIGVLDTGIWPESEVFCDEGLGPVPSRWKGIC 180

Query: 181 ESGELFSPAKACSRKLIGARYFVKGLEAAYGHPLNNSEFQDYWSPRDRSGHGTHVSSVAS 240
           ESGELFSPAKACSRKLIGARYFVKGLEAAYGHPLNNSEFQDYWSPRDRSGHGTHVSSVAS
Sbjct: 181 ESGELFSPAKACSRKLIGARYFVKGLEAAYGHPLNNSEFQDYWSPRDRSGHGTHVSSVAS 240

Query: 241 GFFVPNVSYHGLAVGTVRGGAPHSRIAMYKVCWQVNGGVCADIDILKAIDQAIYDGVDVL 300
           GFFVPNVSYHGLAVGTVRGGAPHSRIAMYKVCWQVNGGVCADIDILKAIDQAIYDGVDVL
Sbjct: 241 GFFVPNVSYHGLAVGTVRGGAPHSRIAMYKVCWQVNGGVCADIDILKAIDQAIYDGVDVL 300

Query: 301 SLSLGPSFPSYSDVDMRNGIAIGAFHAVAKGIVVVGAAGNSGPAAYSVSNIEPWLLTVAA 360
           SLSLGPSFPSYSDVDMRNGIAIGAFHAVAKGIVVVGAAGNSGPAAYSVSNIEPWLLTVAA
Sbjct: 301 SLSLGPSFPSYSDVDMRNGIAIGAFHAVAKGIVVVGAAGNSGPAAYSVSNIEPWLLTVAA 360

Query: 361 SSVDRSFLVAITLGNNWTTMGQGMFSGKLTKFHNLVYPEVSDLDDERACESLSLNDTWAA 420
           SSVD SFLVAITLGNNWTTMGQGMFSGKLTKFHNLVYPEVSDLDDERACESLSLNDTWAA
Sbjct: 361 SSVDCSFLVAITLGNNWTTMGQGMFSGKLTKFHNLVYPEVSDLDDERACESLSLNDTWAA 420

Query: 421 GNVVLCFASDDYNDDTHNTSSSVKKVGGLGLIVAKNPTKAVEPFINNFPCVQISLDIGMQ 480
           GNVVLCFASDDYNDDTHNTSSSVKKVGGLGLIVAKNPTKAVEPFINNFPCVQISLDIGMQ
Sbjct: 421 GNVVLCFASDDYNDDTHNTSSSVKKVGGLGLIVAKNPTKAVEPFINNFPCVQISLDIGMQ 480

Query: 481 ILNYIRSTRNPQVKIGASTTRVGQPLSTTVAYFSSRGPNSVAPAILKPDIAAPGVAILAA 540
           ILNYIRSTRNPQVKIGASTTRVGQPLSTTVAYFSSRGPNSVAPAILKPDIAAPGVAILAA
Sbjct: 481 ILNYIRSTRNPQVKIGASTTRVGQPLSTTVAYFSSRGPNSVAPAILKPDIAAPGVAILAA 540

Query: 541 VPPSDPKDRNSYAFISGTSMATPHVSAIVALLKTLHSHWSPAAIKSAIVTTAWSSDPYGA 600
           VPPSDPKDRNSYAFISGTSMATPHVSAIVALLKTLHSHWSPAAIKSAIVTTAWSSDPYGA
Sbjct: 541 VPPSDPKDRNSYAFISGTSMATPHVSAIVALLKTLHSHWSPAAIKSAIVTTAWSSDPYGA 600

Query: 601 VIFAEGQPMMSARPFDFGGGIVNPNKAVDPGLVYDMGMADYIQYFCAKGYNDSAISGITK 660
           VIFAEGQPMMSARPFDFGGGIVNPNKAVDPGLVYDMGMADYIQYFCAKGYN+SAISGITK
Sbjct: 601 VIFAEGQPMMSARPFDFGGGIVNPNKAVDPGLVYDMGMADYIQYFCAKGYNNSAISGITK 660

Query: 661 RSISCPKRRPSILDINVPSITIPSLTHPVSLTRTVTNVGAINSSYKAAIEAPPGITIAIK 720
           RSISCPKRRPSILDINVPSITIPSLTHPVSLTRTVTNVGAINSSYKAAIEAPPGITIA+K
Sbjct: 661 RSISCPKRRPSILDINVPSITIPSLTHPVSLTRTVTNVGAINSSYKAAIEAPPGITIAVK 720

Query: 721 PRILKFNHKMKSISFTVTISSNHRVTTGYCFGSLTWLDGVHSVRIPVSVRTNI 774
           PRILKFNHKMKSISFTVTISSNHRVTTGYCFGSLTWLDGVHSVRIPVSVRTNI
Sbjct: 721 PRILKFNHKMKSISFTVTISSNHRVTTGYCFGSLTWLDGVHSVRIPVSVRTNI 773

BLAST of Carg15037 vs. NCBI nr
Match: XP_023551712.1 (subtilisin-like protease SBT3.9 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 1535.4 bits (3974), Expect = 0.0e+00
Identity = 766/773 (99.09%), Postives = 771/773 (99.74%), Query Frame = 0

Query: 1   MGSFAHGKSSSTTIFLVCISVFTALDHLCTLHIRVAAKSNVYIVYMGQKPHDNEELLVKA 60
           MGSFAHGKSSSTTIFLVCISVFTALDHLCTLHIRVAAKSNVYIVYMGQKPHDNEELLVKA
Sbjct: 1   MGSFAHGKSSSTTIFLVCISVFTALDHLCTLHIRVAAKSNVYIVYMGQKPHDNEELLVKA 60

Query: 61  HHGVLASVLGSQEGSVDSLVYSYKYGFSGFAAKLTMAQAQMISELPSVVEVIPNRLHKMQ 120
           HHGVLASVLGSQEGSVDSLVYSYKYGFSGFAAKLTMAQAQMISELPSVVEVIPNRLHK+Q
Sbjct: 61  HHGVLASVLGSQEGSVDSLVYSYKYGFSGFAAKLTMAQAQMISELPSVVEVIPNRLHKIQ 120

Query: 121 TTRSWDYLQLSPRFPNSLLRKSRMGSGAIIGVLDTGIWPESEVFCDEGLGPVPSRWKGIC 180
           TTRSWDYLQLSPRFPNSLLRKSRMGSGAIIGVLDTGIWPESEVFCDEGLGPVPSRWKGIC
Sbjct: 121 TTRSWDYLQLSPRFPNSLLRKSRMGSGAIIGVLDTGIWPESEVFCDEGLGPVPSRWKGIC 180

Query: 181 ESGELFSPAKACSRKLIGARYFVKGLEAAYGHPLNNSEFQDYWSPRDRSGHGTHVSSVAS 240
           ESGELFSPAKACSRKLIGARYFVKGLEAAYGHPLNNS+FQDYWSPRDRSGHGTHVSSVAS
Sbjct: 181 ESGELFSPAKACSRKLIGARYFVKGLEAAYGHPLNNSKFQDYWSPRDRSGHGTHVSSVAS 240

Query: 241 GFFVPNVSYHGLAVGTVRGGAPHSRIAMYKVCWQVNGGVCADIDILKAIDQAIYDGVDVL 300
           GFFVPNVSYHGLAVGTVRGGAPHSRIAMYKVCWQVNGGVCADIDILKAIDQAIYDGVDVL
Sbjct: 241 GFFVPNVSYHGLAVGTVRGGAPHSRIAMYKVCWQVNGGVCADIDILKAIDQAIYDGVDVL 300

Query: 301 SLSLGPSFPSYSDVDMRNGIAIGAFHAVAKGIVVVGAAGNSGPAAYSVSNIEPWLLTVAA 360
           SLSLGPSFPSYSDVDMRNGIAIGAFHAVAKGIVVVGAAGNSGPAAYSVSNIEPWLLTVAA
Sbjct: 301 SLSLGPSFPSYSDVDMRNGIAIGAFHAVAKGIVVVGAAGNSGPAAYSVSNIEPWLLTVAA 360

Query: 361 SSVDRSFLVAITLGNNWTTMGQGMFSGKLTKFHNLVYPEVSDLDDERACESLSLNDTWAA 420
           SSVDRSFLVAITLGNNWTTMGQGMFSGKLTKFHNLVYPEVSDLDDERACESLSLNDTWAA
Sbjct: 361 SSVDRSFLVAITLGNNWTTMGQGMFSGKLTKFHNLVYPEVSDLDDERACESLSLNDTWAA 420

Query: 421 GNVVLCFASDDYNDDTHNTSSSVKKVGGLGLIVAKNPTKAVEPFINNFPCVQISLDIGMQ 480
           GNVVLCFASDD+NDDTHNTSSSVKKVGGLGLIVAKNPTKAVEPFINNFPCVQISLD GMQ
Sbjct: 421 GNVVLCFASDDFNDDTHNTSSSVKKVGGLGLIVAKNPTKAVEPFINNFPCVQISLDTGMQ 480

Query: 481 ILNYIRSTRNPQVKIGASTTRVGQPLSTTVAYFSSRGPNSVAPAILKPDIAAPGVAILAA 540
           ILNYIRSTRNPQVKIGASTTRVGQPLSTTVAYFSSRGPNSVAPAILKPDIAAPGVAILAA
Sbjct: 481 ILNYIRSTRNPQVKIGASTTRVGQPLSTTVAYFSSRGPNSVAPAILKPDIAAPGVAILAA 540

Query: 541 VPPSDPKDRNSYAFISGTSMATPHVSAIVALLKTLHSHWSPAAIKSAIVTTAWSSDPYGA 600
           VPPSDPKDRNSYAFISGTSMATPHVSAIVALLKTLHSHWSPAAIKSAIVTTAWSSDPYGA
Sbjct: 541 VPPSDPKDRNSYAFISGTSMATPHVSAIVALLKTLHSHWSPAAIKSAIVTTAWSSDPYGA 600

Query: 601 VIFAEGQPMMSARPFDFGGGIVNPNKAVDPGLVYDMGMADYIQYFCAKGYNDSAISGITK 660
           VIFAEGQPMMSARPFDFGGGIVNPNKAVDPGLVYDMGMADYIQYFCAKGYN+SAISGITK
Sbjct: 601 VIFAEGQPMMSARPFDFGGGIVNPNKAVDPGLVYDMGMADYIQYFCAKGYNNSAISGITK 660

Query: 661 RSISCPKRRPSILDINVPSITIPSLTHPVSLTRTVTNVGAINSSYKAAIEAPPGITIAIK 720
           RSISCPKRRPSILDINVPSITIPSLTHPVSLTRTVTNVGAINSSYKAAIEAPPGITIA+K
Sbjct: 661 RSISCPKRRPSILDINVPSITIPSLTHPVSLTRTVTNVGAINSSYKAAIEAPPGITIAVK 720

Query: 721 PRILKFNHKMKSISFTVTISSNHRVTTGYCFGSLTWLDGVHSVRIPVSVRTNI 774
           PRILKFNHKMKSI FTVTISSNHRVTTGYCFGSLTWLDGVHSVRIPVSVRTNI
Sbjct: 721 PRILKFNHKMKSIPFTVTISSNHRVTTGYCFGSLTWLDGVHSVRIPVSVRTNI 773

BLAST of Carg15037 vs. NCBI nr
Match: XP_022984356.1 (subtilisin-like protease SBT3.9 isoform X1 [Cucurbita maxima])

HSP 1 Score: 1510.4 bits (3909), Expect = 0.0e+00
Identity = 757/773 (97.93%), Postives = 766/773 (99.09%), Query Frame = 0

Query: 1   MGSFAHGKSSSTTIFLVCISVFTALDHLCTLHIRVAAKSNVYIVYMGQKPHDNEELLVKA 60
           MGSFAHGKSSSTTIFLVCISVFTALDHLCTLHIRVAAKSNVYIVYMGQKPHDNEELLVKA
Sbjct: 1   MGSFAHGKSSSTTIFLVCISVFTALDHLCTLHIRVAAKSNVYIVYMGQKPHDNEELLVKA 60

Query: 61  HHGVLASVLGSQEGSVDSLVYSYKYGFSGFAAKLTMAQAQMISELPSVVEVIPNRLHKMQ 120
           HHGVLASVLGSQEGSVDSLVYSYKYGFSGFAAKLTMAQAQMISELPSVVEVIPNRLHK+Q
Sbjct: 61  HHGVLASVLGSQEGSVDSLVYSYKYGFSGFAAKLTMAQAQMISELPSVVEVIPNRLHKIQ 120

Query: 121 TTRSWDYLQLSPRFPNSLLRKSRMGSGAIIGVLDTGIWPESEVFCDEGLGPVPSRWKGIC 180
           TTRSWDYLQLSPRFPNSLLRKSRMGSGAIIGVLDTGIWPESEVFCDEGLGPVPSRWKGIC
Sbjct: 121 TTRSWDYLQLSPRFPNSLLRKSRMGSGAIIGVLDTGIWPESEVFCDEGLGPVPSRWKGIC 180

Query: 181 ESGELFSPAKACSRKLIGARYFVKGLEAAYGHPLNNSEFQDYWSPRDRSGHGTHVSSVAS 240
           ESGELFSPAKACSRKLIGARYFVKGLEAAYGHPLNNSEFQDY SPRDRSGHGTHVSSVAS
Sbjct: 181 ESGELFSPAKACSRKLIGARYFVKGLEAAYGHPLNNSEFQDYLSPRDRSGHGTHVSSVAS 240

Query: 241 GFFVPNVSYHGLAVGTVRGGAPHSRIAMYKVCWQVNGGVCADIDILKAIDQAIYDGVDVL 300
           GFFVPNVSYHGLAVGTVRGGAPHSRIAMYKVCWQVNGGVCADIDILKAIDQAIYDGVDVL
Sbjct: 241 GFFVPNVSYHGLAVGTVRGGAPHSRIAMYKVCWQVNGGVCADIDILKAIDQAIYDGVDVL 300

Query: 301 SLSLGPSFPSYSDVDMRNGIAIGAFHAVAKGIVVVGAAGNSGPAAYSVSNIEPWLLTVAA 360
           SLSLGPSFPSYSDVDMRNGIAIGAFHAVAKGIVVVGAAGNSGPAAYSVSNIEPWLLTVAA
Sbjct: 301 SLSLGPSFPSYSDVDMRNGIAIGAFHAVAKGIVVVGAAGNSGPAAYSVSNIEPWLLTVAA 360

Query: 361 SSVDRSFLVAITLGNNWTTMGQGMFSGKLTKFHNLVYPEVSDLDDERACESLSLNDTWAA 420
           SSVDRSFLVAITLGNNWTTMGQGMFSGKLTKF NLVYPEVSDLDDERACESLSLNDTWA+
Sbjct: 361 SSVDRSFLVAITLGNNWTTMGQGMFSGKLTKFINLVYPEVSDLDDERACESLSLNDTWAS 420

Query: 421 GNVVLCFASDDYNDDTHNTSSSVKKVGGLGLIVAKNPTKAVEPFINNFPCVQISLDIGMQ 480
           GNVVLCFASDDYND+THNTS SVKKVGGLGLIVAKNPTKAVEPFI+NFPCVQISLDIGMQ
Sbjct: 421 GNVVLCFASDDYNDNTHNTSWSVKKVGGLGLIVAKNPTKAVEPFIDNFPCVQISLDIGMQ 480

Query: 481 ILNYIRSTRNPQVKIGASTTRVGQPLSTTVAYFSSRGPNSVAPAILKPDIAAPGVAILAA 540
           ILNYIRSTRNPQVKIGASTTRVGQPLSTTVAYFSSRGPNSVAPAILKPDIAAPGVAILAA
Sbjct: 481 ILNYIRSTRNPQVKIGASTTRVGQPLSTTVAYFSSRGPNSVAPAILKPDIAAPGVAILAA 540

Query: 541 VPPSDPKDRNSYAFISGTSMATPHVSAIVALLKTLHSHWSPAAIKSAIVTTAWSSDPYGA 600
           VPPSDPK+RNSYAFISGTSMATPHVSAIVALLKTLHSHWSPAAIKSAIVTTAWSSDPYGA
Sbjct: 541 VPPSDPKNRNSYAFISGTSMATPHVSAIVALLKTLHSHWSPAAIKSAIVTTAWSSDPYGA 600

Query: 601 VIFAEGQPMMSARPFDFGGGIVNPNKAVDPGLVYDMGMADYIQYFCAKGYNDSAISGITK 660
           VIFAEG+PMMSARPFDFGGGIVNPNKAVDPGLVYDMGMADYIQYFCAKGYN+SAISGI+K
Sbjct: 601 VIFAEGRPMMSARPFDFGGGIVNPNKAVDPGLVYDMGMADYIQYFCAKGYNNSAISGISK 660

Query: 661 RSISCPKRRPSILDINVPSITIPSLTHPVSLTRTVTNVGAINSSYKAAIEAPPGITIAIK 720
           RSISCPKRRPSILDINVPSITIPSLTH VSLTRTVTNVGAINSSYKAAIE PPGITIA+K
Sbjct: 661 RSISCPKRRPSILDINVPSITIPSLTHSVSLTRTVTNVGAINSSYKAAIEVPPGITIAVK 720

Query: 721 PRILKFNHKMKSISFTVTISSNHRVTTGYCFGSLTWLDGVHSVRIPVSVRTNI 774
           PRILKFNHK KSISFTVTISSN RVTTGYCFGSLTWLDGVHSVRIPVSVRTNI
Sbjct: 721 PRILKFNHKTKSISFTVTISSNQRVTTGYCFGSLTWLDGVHSVRIPVSVRTNI 773

BLAST of Carg15037 vs. NCBI nr
Match: KAG6576624.1 (Subtilisin-like protease 3.3, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 1501.1 bits (3885), Expect = 0.0e+00
Identity = 748/750 (99.73%), Postives = 750/750 (100.00%), Query Frame = 0

Query: 24  ALDHLCTLHIRVAAKSNVYIVYMGQKPHDNEELLVKAHHGVLASVLGSQEGSVDSLVYSY 83
           ALDHLCTLHIRVAAKSNVYIVYMGQKPHDNEELLVKAHHGVLASVLGSQEGSVDSLVYSY
Sbjct: 8   ALDHLCTLHIRVAAKSNVYIVYMGQKPHDNEELLVKAHHGVLASVLGSQEGSVDSLVYSY 67

Query: 84  KYGFSGFAAKLTMAQAQMISELPSVVEVIPNRLHKMQTTRSWDYLQLSPRFPNSLLRKSR 143
           KYGFSGFAAKLTMAQAQMISELPSVVEVIPNRLHKMQTTRSWDYLQLSPRFPNSLLRKSR
Sbjct: 68  KYGFSGFAAKLTMAQAQMISELPSVVEVIPNRLHKMQTTRSWDYLQLSPRFPNSLLRKSR 127

Query: 144 MGSGAIIGVLDTGIWPESEVFCDEGLGPVPSRWKGICESGELFSPAKACSRKLIGARYFV 203
           MGSGAIIGVLDTGIWPESEVFCDEGLGPVPSRWKGICESGELFSPAKACSRKLIGARYFV
Sbjct: 128 MGSGAIIGVLDTGIWPESEVFCDEGLGPVPSRWKGICESGELFSPAKACSRKLIGARYFV 187

Query: 204 KGLEAAYGHPLNNSEFQDYWSPRDRSGHGTHVSSVASGFFVPNVSYHGLAVGTVRGGAPH 263
           KGLEAAYGHPLNNSEFQDYWSPRDRSGHGTHVSSVASGFFVPNVSYHGLAVGTVRGGAPH
Sbjct: 188 KGLEAAYGHPLNNSEFQDYWSPRDRSGHGTHVSSVASGFFVPNVSYHGLAVGTVRGGAPH 247

Query: 264 SRIAMYKVCWQVNGGVCADIDILKAIDQAIYDGVDVLSLSLGPSFPSYSDVDMRNGIAIG 323
           SRIAMYKVCWQVNGGVCADIDILKAIDQAIYDGVDVLSLSLGPSFPSYSDVDMRNGIAIG
Sbjct: 248 SRIAMYKVCWQVNGGVCADIDILKAIDQAIYDGVDVLSLSLGPSFPSYSDVDMRNGIAIG 307

Query: 324 AFHAVAKGIVVVGAAGNSGPAAYSVSNIEPWLLTVAASSVDRSFLVAITLGNNWTTMGQG 383
           AFHAVAKGIVVVGAAGNSGPAAYSVSNIEPWLLTVAASSVDRSFLVAITLGNNWTTMGQG
Sbjct: 308 AFHAVAKGIVVVGAAGNSGPAAYSVSNIEPWLLTVAASSVDRSFLVAITLGNNWTTMGQG 367

Query: 384 MFSGKLTKFHNLVYPEVSDLDDERACESLSLNDTWAAGNVVLCFASDDYNDDTHNTSSSV 443
           MFSGKLTKFHNLVYPEVSDLDDERACESLSLNDTWAAGNVVLCFASDDYNDDTHNTSSSV
Sbjct: 368 MFSGKLTKFHNLVYPEVSDLDDERACESLSLNDTWAAGNVVLCFASDDYNDDTHNTSSSV 427

Query: 444 KKVGGLGLIVAKNPTKAVEPFINNFPCVQISLDIGMQILNYIRSTRNPQVKIGASTTRVG 503
           KKVGGLGLIVAKNPTKAVEPF+NNFPCVQISLDIGMQILNYIRSTRNPQVKIGASTTRVG
Sbjct: 428 KKVGGLGLIVAKNPTKAVEPFVNNFPCVQISLDIGMQILNYIRSTRNPQVKIGASTTRVG 487

Query: 504 QPLSTTVAYFSSRGPNSVAPAILKPDIAAPGVAILAAVPPSDPKDRNSYAFISGTSMATP 563
           QPLSTTVAYFSSRGPNSVAPAILKPDIAAPGVAILAAVPPSDPKDRNSYAFISGTSMATP
Sbjct: 488 QPLSTTVAYFSSRGPNSVAPAILKPDIAAPGVAILAAVPPSDPKDRNSYAFISGTSMATP 547

Query: 564 HVSAIVALLKTLHSHWSPAAIKSAIVTTAWSSDPYGAVIFAEGQPMMSARPFDFGGGIVN 623
           HVSAIVALLKTLHSHWSPAAIKSAIVTTAWSSDPYGAVIFAEGQPMMSARPFDFGGGIVN
Sbjct: 548 HVSAIVALLKTLHSHWSPAAIKSAIVTTAWSSDPYGAVIFAEGQPMMSARPFDFGGGIVN 607

Query: 624 PNKAVDPGLVYDMGMADYIQYFCAKGYNDSAISGITKRSISCPKRRPSILDINVPSITIP 683
           PNKAVDPGLVYDMGMADYIQYFCAKGYN+SAISGITKRSISCPKRRPSILDINVPSITIP
Sbjct: 608 PNKAVDPGLVYDMGMADYIQYFCAKGYNNSAISGITKRSISCPKRRPSILDINVPSITIP 667

Query: 684 SLTHPVSLTRTVTNVGAINSSYKAAIEAPPGITIAIKPRILKFNHKMKSISFTVTISSNH 743
           SLTHPVSLTRTVTNVGAINSSYKAAIEAPPGITIAIKPRILKFNHKMKSISFTVTISSNH
Sbjct: 668 SLTHPVSLTRTVTNVGAINSSYKAAIEAPPGITIAIKPRILKFNHKMKSISFTVTISSNH 727

Query: 744 RVTTGYCFGSLTWLDGVHSVRIPVSVRTNI 774
           RVTTGYCFGSLTWLDGVHSVRIPVSVRTNI
Sbjct: 728 RVTTGYCFGSLTWLDGVHSVRIPVSVRTNI 757

BLAST of Carg15037 vs. ExPASy Swiss-Prot
Match: Q9MAP5 (Subtilisin-like protease SBT3.3 OS=Arabidopsis thaliana OX=3702 GN=SBT3.3 PE=2 SV=1)

HSP 1 Score: 823.9 bits (2127), Expect = 1.5e-237
Identity = 422/773 (54.59%), Postives = 548/773 (70.89%), Query Frame = 0

Query: 8   KSSSTTIFLVCISVFTALDHLCTLHIRVAAKSNVYIVYMGQKPHDNEELLVKAHHGVLAS 67
           +S  ++I LV +S+ T L+       R   +S V+IVY+G+K H + E + ++HH +LAS
Sbjct: 2   RSFRSSILLVLLSLITVLN---ATRARSETESKVHIVYLGEKKHHDPEFVTESHHQMLAS 61

Query: 68  VLGSQEGSVDSLVYSYKYGFSGFAAKLTMAQAQMISELPSVVEVIPNRLHKMQTTRSWDY 127
           +LGS++ + DS+VYSY++GFSGFAAKLT +QA+ I++LP VV VIP+  H++ TTR+W+Y
Sbjct: 62  LLGSKKDADDSMVYSYRHGFSGFAAKLTKSQAKKIADLPEVVHVIPDGFHELATTRTWEY 121

Query: 128 LQLSPRFPNSLLRKSRMGSGAIIGVLDTGIWPESEVFCDEGLGPVPSRWKGICESGELFS 187
           L LS   P +LL  + MG   IIGV+DTG+WPESE F D G+GP+P +WKG CESGE F 
Sbjct: 122 LGLSSANPKNLLNDTNMGDQVIIGVIDTGVWPESESFNDNGVGPIPRKWKGGCESGENFR 181

Query: 188 PAKACSRKLIGARYFVKGLEAAYGHPLNNSEFQDYWSPRDRSGHGTHVSSVASGFFVPNV 247
            +  C+RKLIGA+YF+ G   A     N +E +DY S RD  GHGTHV+S+A G FVPNV
Sbjct: 182 -STDCNRKLIGAKYFINGF-LAENKGFNTTESRDYISARDFDGHGTHVASIAGGSFVPNV 241

Query: 248 SYHGLAVGTVRGGAPHSRIAMYKVCW---QVNGGVCADIDILKAIDQAIYDGVDVLSLSL 307
           SY GLA GT+RGGAP +RIAMYK CW   ++ G  C+D DI+KAID+AI+DGVDVLS+SL
Sbjct: 242 SYKGLAGGTLRGGAPRARIAMYKACWFHEELKGVTCSDSDIMKAIDEAIHDGVDVLSISL 301

Query: 308 GPSFPSYSDVDMRNGIAIGAFHAVAKGIVVVGAAGNSGPAAYSVSNIEPWLLTVAASSVD 367
               P  S+ D+R+  A G FHAVAKGIVVV A GN GPAA +V NI PW+LTVAA+++D
Sbjct: 302 VGQIPLNSETDIRDEFATGLFHAVAKGIVVVCAGGNDGPAAQTVVNIAPWILTVAATTLD 361

Query: 368 RSFLVAITLGNNWTTMGQGMFSGKLTKFHNLVYPEVSDLDDER---ACESLSLNDTWA-A 427
           RSF   ITLGNN   +GQ  ++G      +LVYPE +  ++E     CESL+LN  +  A
Sbjct: 362 RSFPTPITLGNNKVILGQATYTGPELGLTSLVYPENARNNNETFSGVCESLNLNPNYTMA 421

Query: 428 GNVVLCFASDDYNDDTHNTSSSVKKVGGLGLIVAKNPTKAVEPFINNFPCVQISLDIGMQ 487
             VVLCF +   N      +S VK  GGLGLI+++NP   + P  ++FPCV +  ++G  
Sbjct: 422 MKVVLCFTASRTNAAISRAASFVKAAGGLGLIISRNPVYTLSPCNDDFPCVAVDYELGTD 481

Query: 488 ILNYIRSTRNPQVKIGASTTRVGQPLSTTVAYFSSRGPNSVAPAILKPDIAAPGVAILAA 547
           IL+YIRSTR+P VKI  S T  GQP+ T V  FSSRGPNS++PAILKPDIAAPGV ILAA
Sbjct: 482 ILSYIRSTRSPVVKIQRSRTLSGQPVGTKVVNFSSRGPNSMSPAILKPDIAAPGVRILAA 541

Query: 548 VPPSDPKDRNSYAFISGTSMATPHVSAIVALLKTLHSHWSPAAIKSAIVTTAWSSDPYGA 607
             P+D  +   +A +SGTSMATP +S ++ALLK LH  WSPAA +SAIVTTAW +DP+G 
Sbjct: 542 TSPNDTLNVGGFAMLSGTSMATPVISGVIALLKALHPEWSPAAFRSAIVTTAWRTDPFGE 601

Query: 608 VIFAEGQPMMSARPFDFGGGIVNPNKAVDPGLVYDMGMADYIQYFCAKGYNDSAISGITK 667
            IFAEG     + PFD+GGGIVNP KA +PGL+YDMG  DYI Y C+ GYNDS+IS +  
Sbjct: 602 QIFAEGSSRKVSDPFDYGGGIVNPEKAAEPGLIYDMGPQDYILYLCSAGYNDSSISQLVG 661

Query: 668 RSISCPKRRPSILDINVPSITIPSLTHPVSLTRTVTNVGAINSSYKAAIEAPPGITIAIK 727
           +   C   +PS+LD+N+PSITIP+L   V+LTRTVTNVG ++S YK ++E P G+ + + 
Sbjct: 662 QITVCSNPKPSVLDVNLPSITIPNLKDEVTLTRTVTNVGLVDSVYKVSVEPPLGVRVVVT 721

Query: 728 PRILKFNHKMKSISFTVTISSNHRVTTGYCFGSLTWLDGVHSVRIPVSVRTNI 774
           P  L FN K  S+SFTV +S+ H++ TGY FGSLTW D VH+V IP+SVRT I
Sbjct: 722 PETLVFNSKTISVSFTVRVSTTHKINTGYYFGSLTWTDSVHNVVIPLSVRTQI 769

BLAST of Carg15037 vs. ExPASy Swiss-Prot
Match: Q9ZSB0 (Subtilisin-like protease SBT3.9 OS=Arabidopsis thaliana OX=3702 GN=SBT3.9 PE=3 SV=1)

HSP 1 Score: 815.5 bits (2105), Expect = 5.3e-235
Identity = 422/740 (57.03%), Postives = 540/740 (72.97%), Query Frame = 0

Query: 35  VAAKSNVYIVYMGQKPHDNEELLVKAHHGVLASVLGSQEGSVDSLVYSYKYGFSGFAAKL 94
           V A+S VY+VY+G+K HDN E + ++HH +L S+LGS+E  +DS+VYSY++GFSGFAAKL
Sbjct: 23  VVAESKVYVVYLGEKEHDNPESVTESHHQMLWSLLGSKEAVLDSIVYSYRHGFSGFAAKL 82

Query: 95  TMAQAQMISELPSVVEVIPNRLHKMQTTRSWDYLQLSPRFPNSLLRKSRMGSGAIIGVLD 154
           T +QAQ ISELP VV+VIPN L++M TTR+WDYL +SP   +SLL+K+ MG   I+GV+D
Sbjct: 83  TESQAQQISELPEVVQVIPNTLYEMTTTRTWDYLGVSPGNSDSLLQKANMGYNVIVGVID 142

Query: 155 TGIWPESEVFCDEGLGPVPSRWKGICESGELFSPAKACSRKLIGARYFVKGLEAAYGHPL 214
           +G+WPESE+F D+G GP+PSRWKG CESGELF+ +  C+RKLIGA+YFV GL A +G  +
Sbjct: 143 SGVWPESEMFNDKGFGPIPSRWKGGCESGELFNASIHCNRKLIGAKYFVDGLVAEFG-VV 202

Query: 215 NNSEFQDYWSPRDRSGHGTHVSSVASGFFVPNVSYHGLAVGTVRGGAPHSRIAMYKVCWQ 274
           N ++  +Y SPRD +GHGTHV+S   G F+PNVSY GL  GT RGGAP   IA+YK CW 
Sbjct: 203 NRTQNPEYLSPRDFAGHGTHVASTIGGSFLPNVSYVGLGRGTARGGAPGVHIAVYKACW- 262

Query: 275 VNGGVCADIDILKAIDQAIYDGVDVLSLSLGPSFPSYSDVDMRNGIAIGAFHAVAKGIVV 334
              G C+  D+LKA+D+AI+DGVD+LSLSLGPS P + + +     ++GAFHAVAKGI V
Sbjct: 263 --SGYCSGADVLKAMDEAIHDGVDILSLSLGPSVPLFPETE---HTSVGAFHAVAKGIPV 322

Query: 335 VGAAGNSGPAAYSVSNIEPWLLTVAASSVDRSFLVAITLGNNWTTMGQGMFSGKLTKFHN 394
           V AAGN+GP A ++SN+ PW+LTVAA++ DRSF  AITLGNN T +GQ ++ G    F  
Sbjct: 323 VIAAGNAGPTAQTISNVAPWVLTVAATTQDRSFPTAITLGNNITILGQAIYGGPELGFVG 382

Query: 395 LVYPEVSDLDDERACESLSLN-DTWAAGNVVLCFASDDYNDDTHNTSSSVKKVGGLGLIV 454
           L YPE S L  +  CE LS N ++   G VVLCFA+   +  ++   ++V   GGLGLI+
Sbjct: 383 LTYPE-SPLSGD--CEKLSANPNSTMEGKVVLCFAA---STPSNAAIAAVINAGGLGLIM 442

Query: 455 AKNPTKAVEPFINNFPCVQISLDIGMQILNYIRSTRNPQVKIGASTTRVGQPLSTTVAYF 514
           AKNPT ++ P    FP V I  ++G  IL YIRSTR+P VKI AS T  GQ +ST VA F
Sbjct: 443 AKNPTHSLTP-TRKFPWVSIDFELGTDILFYIRSTRSPIVKIQASKTLFGQSVSTKVATF 502

Query: 515 SSRGPNSVAPAILKPDIAAPGVAILAAVPPSDPKDRNSYAFISGTSMATPHVSAIVALLK 574
           SSRGPNSV+PAILKPDIAAPGV ILAA+ P+   +   +A +SGTSMATP VS +V LLK
Sbjct: 503 SSRGPNSVSPAILKPDIAAPGVNILAAISPNSSINDGGFAMMSGTSMATPVVSGVVVLLK 562

Query: 575 TLHSHWSPAAIKSAIVTTAWSSDPYGAVIFAEGQPMMSARPFDFGGGIVNPNKAVDPGLV 634
           +LH  WSP+AIKSAIVTTAW +DP G  IFA+G     A PFD+GGG++NP KAV PGL+
Sbjct: 563 SLHPDWSPSAIKSAIVTTAWRTDPSGEPIFADGSSRKLADPFDYGGGLINPEKAVKPGLI 622

Query: 635 YDMGMADYIQYFCAKGYNDSAISGITKRSISCPKRRPSILDINVPSITIPSLTHPVSLTR 694
           YDM   DY+ Y C+  Y+D +IS +  +   CP  +PS+LD+N+PSITIP+L   V+LTR
Sbjct: 623 YDMTTDDYVMYMCSVDYSDISISRVLGKITVCPNPKPSVLDLNLPSITIPNLRGEVTLTR 682

Query: 695 TVTNVGAINSSYKAAIEAPPGITIAIKPRILKFNHKMKSISFTVTISSNHRVTTGYCFGS 754
           TVTNVG +NS YK  I+ P GI +A+ P  L F++     SFTV +S+ H+V TGY FGS
Sbjct: 683 TVTNVGPVNSVYKVVIDPPTGINVAVTPAELVFDYTTTKRSFTVRVSTTHKVNTGYYFGS 742

Query: 755 LTWLDGVHSVRIPVSVRTNI 774
           LTW D +H+V IPVSVRT I
Sbjct: 743 LTWTDNMHNVAIPVSVRTQI 748

BLAST of Carg15037 vs. ExPASy Swiss-Prot
Match: Q9ZSB1 (Subtilisin-like protease SBT3.10 OS=Arabidopsis thaliana OX=3702 GN=SBT3.10 PE=3 SV=2)

HSP 1 Score: 812.8 bits (2098), Expect = 3.4e-234
Identity = 422/740 (57.03%), Postives = 534/740 (72.16%), Query Frame = 0

Query: 35  VAAKSNVYIVYMGQKPHDNEELLVKAHHGVLASVLGSQEGSVDSLVYSYKYGFSGFAAKL 94
           V A+S VY+VY+G+K HDN E + ++HH +L S+LGS+E  +DS+VYSY++GFSGFAAKL
Sbjct: 23  VVAESKVYVVYLGEKEHDNPESVTESHHQMLWSLLGSKEAVLDSIVYSYRHGFSGFAAKL 82

Query: 95  TMAQAQMISELPSVVEVIPNRLHKMQTTRSWDYLQLSPRFPNSLLRKSRMGSGAIIGVLD 154
           T +QAQ ISELP VV+VIPN L++M TTR+WDYL +SP   +SLL+K+ MG   I+GV+D
Sbjct: 83  TESQAQQISELPEVVQVIPNTLYEMTTTRTWDYLGVSPGNSDSLLQKANMGYNVIVGVID 142

Query: 155 TGIWPESEVFCDEGLGPVPSRWKGICESGELFSPAKACSRKLIGARYFVKGLEAAYGHPL 214
           TG+WPESE+F D+G GP+PSRWKG CESGELF+ +  C+RKLIGA+YF+    A +G  L
Sbjct: 143 TGVWPESEMFNDKGYGPIPSRWKGGCESGELFNGSIHCNRKLIGAKYFIDANNAQFG-VL 202

Query: 215 NNSEFQDYWSPRDRSGHGTHVSSVASGFFVPNVSYHGLAVGTVRGGAPHSRIAMYKVCWQ 274
           N +E  DY SPRD +GHGTHV+S   G F+PNVSY GL  GT RGGAP   IA+YK CW 
Sbjct: 203 NKTENPDYLSPRDFNGHGTHVASTIGGSFLPNVSYLGLGRGTARGGAPGVHIAVYKACWV 262

Query: 275 VNGGVCADIDILKAIDQAIYDGVDVLSLSLGPSFPSYSDVDMRNGIAIGAFHAVAKGIVV 334
             G  C+  D+LKA+D+AI+DGVD+LSLSL  S P + + D R   ++GAFHAVAKGI V
Sbjct: 263 QRG--CSGADVLKAMDEAIHDGVDILSLSLQTSVPLFPETDARELTSVGAFHAVAKGIPV 322

Query: 335 VGAAGNSGPAAYSVSNIEPWLLTVAASSVDRSFLVAITLGNNWTTMGQGMFSGKLTKFHN 394
           V AA N+GP A ++SN+ PW+LTVAA++ DRSF  AITLGNN T +GQ +F G    F  
Sbjct: 323 VAAASNAGPTAQTLSNVAPWVLTVAATTQDRSFPTAITLGNNITILGQAIFGGSELGFVG 382

Query: 395 LVYPEVSDLDDERACESLSLNDTWA-AGNVVLCFASDDYNDDTHNTSSSVKKVGGLGLIV 454
           L YPE S L  +  CE LS N   A  G VVLCFA+   +  ++   ++V   GGLGLI+
Sbjct: 383 LTYPE-SPLSGD--CEKLSANPKSAMEGKVVLCFAA---STPSNAAITAVINAGGLGLIM 442

Query: 455 AKNPTKAVEPFINNFPCVQISLDIGMQILNYIRSTRNPQVKIGASTTRVGQPLSTTVAYF 514
           A+NPT  + P + NFP V +  ++G  IL YIRSTR+P V I AS T  GQ +ST VA F
Sbjct: 443 ARNPTHLLRP-LRNFPYVSVDFELGTDILFYIRSTRSPIVNIQASRTLFGQSVSTKVATF 502

Query: 515 SSRGPNSVAPAILKPDIAAPGVAILAAVPPSDPKDRNSYAFISGTSMATPHVSAIVALLK 574
           SSRGPNSV+PAILKPDIAAPGV ILAA+ P+   D   +A +SGTSMATP VS +V LLK
Sbjct: 503 SSRGPNSVSPAILKPDIAAPGVNILAAISPNSIND-GGFAMMSGTSMATPVVSGVVVLLK 562

Query: 575 TLHSHWSPAAIKSAIVTTAWSSDPYGAVIFAEGQPMMSARPFDFGGGIVNPNKAVDPGLV 634
           +LH  WSP+AIKSAIVTTAW +DP G  IFA+G     A PFD+GGG++NP KAV PGL+
Sbjct: 563 SLHPDWSPSAIKSAIVTTAWRTDPSGEPIFADGSSRKLADPFDYGGGLINPEKAVKPGLI 622

Query: 635 YDMGMADYIQYFCAKGYNDSAISGITKRSISCPKRRPSILDINVPSITIPSLTHPVSLTR 694
           YDM   DY+ Y C+  Y+D +IS +  +   CP  +PS+LD+N+PSITIP+L   V+LTR
Sbjct: 623 YDMTTDDYVMYMCSVDYSDISISRVLGKITVCPNPKPSVLDLNLPSITIPNLRGEVTLTR 682

Query: 695 TVTNVGAINSSYKAAIEAPPGITIAIKPRILKFNHKMKSISFTVTISSNHRVTTGYCFGS 754
           TVTNVG +NS YK  I+ P G+ +A+ P  L F+      SFTV +S+ H+V TGY FGS
Sbjct: 683 TVTNVGPVNSVYKVVIDPPTGVNVAVTPTELVFDSTTTKRSFTVRVSTTHKVNTGYYFGS 742

Query: 755 LTWLDGVHSVRIPVSVRTNI 774
           LTW D +H+V IPVSVRT I
Sbjct: 743 LTWTDTLHNVAIPVSVRTQI 751

BLAST of Carg15037 vs. ExPASy Swiss-Prot
Match: Q9SZY3 (Subtilisin-like protease SBT3.8 OS=Arabidopsis thaliana OX=3702 GN=SBT3.8 PE=3 SV=1)

HSP 1 Score: 802.7 bits (2072), Expect = 3.5e-231
Identity = 413/773 (53.43%), Postives = 539/773 (69.73%), Query Frame = 0

Query: 8   KSSSTTIFLVCISVFTALDHLCTLHIRVAAKSNVYIVYMGQKPHDNEELLVKAHHGVLAS 67
           KS  T IF+  I     L+ L T      A+S V+IVY+G+K HD+ E + ++HH +L S
Sbjct: 2   KSCRTLIFVAII-----LNGLSTFVAHAGAESKVHIVYLGEKQHDDPEFVTESHHRMLWS 61

Query: 68  VLGSQEGSVDSLVYSYKYGFSGFAAKLTMAQAQMISELPSVVEVIPNRLHKMQTTRSWDY 127
           +LGS+E +  S+V+SY++GFSGFAAKLT +QA+ +++LP VV V P+  +++ TTR+WDY
Sbjct: 62  LLGSKEDAHSSMVHSYRHGFSGFAAKLTKSQAKKLADLPEVVHVTPDSFYQLDTTRTWDY 121

Query: 128 LQLSPRFPNSLLRKSRMGSGAIIGVLDTGIWPESEVFCDEGLGPVPSRWKGICESGELFS 187
           L LS   P +LL  + MG   IIG++D+G+WPESEVF D G+GPVPS WKG C SGE F+
Sbjct: 122 LGLSVANPKNLLNDTNMGEEVIIGIVDSGVWPESEVFNDNGIGPVPSHWKGGCVSGENFT 181

Query: 188 PAKACSRKLIGARYFVKGLEAAYGHPLNNSEFQDYWSPRDRSGHGTHVSSVASGFFVPNV 247
            ++ C++KLIGA+YF+ G  A +    N++E  D+ SPRDRSGHGTHV+++A G +VP++
Sbjct: 182 SSQ-CNKKLIGAKYFINGFLATH-ESFNSTESLDFISPRDRSGHGTHVATIAGGSYVPSI 241

Query: 248 SYHGLAVGTVRGGAPHSRIAMYKVCWQVNG---GVCADIDILKAIDQAIYDGVDVLSLSL 307
           SY GLA GTVRGGAP +RIAMYK CW ++      C+  DILKA+D+A++DGVDVLSLS+
Sbjct: 242 SYKGLAGGTVRGGAPRARIAMYKACWYLDRFDINTCSSADILKAMDEAMHDGVDVLSLSI 301

Query: 308 GPSFPSYSDVDMRNGIAIGAFHAVAKGIVVVGAAGNSGPAAYSVSNIEPWLLTVAASSVD 367
           G  FP + + D+R  IA GAFHAV KGI VV + GNSGPAA +V N  PW+LTVAA+++D
Sbjct: 302 GYRFPYFPETDVRAVIATGAFHAVLKGITVVCSGGNSGPAAQTVGNTAPWILTVAATTLD 361

Query: 368 RSFLVAITLGNNWTTMGQGMFSGKLTKFHNLVYPEVSDLDDER---ACESLSLNDTWA-A 427
           RSF   ITLGNN   +GQ M++G    F +LVYPE     +E     CE L  N     A
Sbjct: 362 RSFPTPITLGNNKLILGQAMYTGPELGFTSLVYPENPGNSNESFSGDCELLFFNSNHTMA 421

Query: 428 GNVVLCFASDDYNDDTHNTSSSVKKVGGLGLIVAKNPTKAVEPFINNFPCVQISLDIGMQ 487
           G VVLCF +        +  S VK+ GGLG+IVA+NP   + P  ++FPCV +  ++G  
Sbjct: 422 GKVVLCFTTSTRYITVSSAVSYVKEAGGLGVIVARNPGDNLSPCEDDFPCVAVDYELGTD 481

Query: 488 ILNYIRSTRNPQVKIGASTTRVGQPLSTTVAYFSSRGPNSVAPAILKPDIAAPGVAILAA 547
           IL YIRST  P VKI  S T VGQP+ T VA FSSRGPNS+ PAILKPDIAAPGV+ILAA
Sbjct: 482 ILLYIRSTGLPVVKIQPSKTLVGQPVGTKVADFSSRGPNSIEPAILKPDIAAPGVSILAA 541

Query: 548 VPPSDPKDRNSYAFISGTSMATPHVSAIVALLKTLHSHWSPAAIKSAIVTTAWSSDPYGA 607
              +   +   + F+SGTSMA P +S +VALLK LH  WSPAAI+SAIVTTAW +DP+G 
Sbjct: 542 TTTNKTFNDRGFIFLSGTSMAAPTISGVVALLKALHRDWSPAAIRSAIVTTAWRTDPFGE 601

Query: 608 VIFAEGQPMMSARPFDFGGGIVNPNKAVDPGLVYDMGMADYIQYFCAKGYNDSAISGITK 667
            IFAEG P   A PFD+GGG+VNP KA  PGLVYD+G+ DY+ Y C+ GYN+++IS +  
Sbjct: 602 QIFAEGSPRKLADPFDYGGGLVNPEKAAKPGLVYDLGLEDYVLYMCSVGYNETSISQLVG 661

Query: 668 RSISCPKRRPSILDINVPSITIPSLTHPVSLTRTVTNVGAINSSYKAAIEAPPGITIAIK 727
           +   C   +PS+LD N+PSITIP+L   V+LTRT+TNVG + S YK  IE P GI + + 
Sbjct: 662 KGTVCSNPKPSVLDFNLPSITIPNLKDEVTLTRTLTNVGQLESVYKVVIEPPIGIQVTVT 721

Query: 728 PRILKFNHKMKSISFTVTISSNHRVTTGYCFGSLTWLDGVHSVRIPVSVRTNI 774
           P  L FN   K +SF V +S+ H++ TGY FGSLTW D +H+V IP+SVRT I
Sbjct: 722 PETLLFNSTTKRVSFKVKVSTTHKINTGYFFGSLTWSDSLHNVTIPLSVRTQI 767

BLAST of Carg15037 vs. ExPASy Swiss-Prot
Match: Q8L7I2 (Subtilisin-like protease SBT3.6 OS=Arabidopsis thaliana OX=3702 GN=SBT3.6 PE=2 SV=1)

HSP 1 Score: 802.4 bits (2071), Expect = 4.6e-231
Identity = 406/769 (52.80%), Postives = 542/769 (70.48%), Query Frame = 0

Query: 12  TTIFLVCISVFTALDHLCTLHIRVAAKSNVYIVYMGQKPHDNEELLVKAHHGVLASVLGS 71
           T+I++V +S+   L+   +     +AK  V+IVY+G+K HD+ E + ++HH +L S+LGS
Sbjct: 6   TSIYVV-LSLVIFLNVQRSFVAESSAKRKVHIVYLGEKQHDDPEFVTESHHRMLWSLLGS 65

Query: 72  QEGSVDSLVYSYKYGFSGFAAKLTMAQAQMISELPSVVEVIPNRLHKMQTTRSWDYLQLS 131
           +E + DS+VYSY++GFSGFAAKLT +QA+ I++LP VV VIP+  +K+ TTR+WDYL LS
Sbjct: 66  KEDANDSMVYSYRHGFSGFAAKLTESQAKKIADLPDVVHVIPDSFYKLATTRTWDYLGLS 125

Query: 132 PRFPNSLLRKSRMGSGAIIGVLDTGIWPESEVFCDEGLGPVPSRWKGICESGELFSPAKA 191
              P SLL ++ MG   IIGV+DTG+WPESEVF D G GPVPS WKG CE+GE F+ +  
Sbjct: 126 AANPKSLLHETNMGEQIIIGVIDTGVWPESEVFNDSGFGPVPSHWKGGCETGENFNSSN- 185

Query: 192 CSRKLIGARYFVKGLEAAYGHPLNNSEFQDYWSPRDRSGHGTHVSSVASGFFVPNVSYHG 251
           C++KLIGA+YF+ G   A     N++   D+ SPRD  GHGTHVS++A G FVPN+SY G
Sbjct: 186 CNKKLIGAKYFINGF-LAENESFNSTNSLDFISPRDLDGHGTHVSTIAGGSFVPNISYKG 245

Query: 252 LAVGTVRGGAPHSRIAMYKVCWQVNGG---VCADIDILKAIDQAIYDGVDVLSLSLGPSF 311
           LA GTVRGGAP + IAMYK CW ++      C+  DILKA+D+A++DGVDVLS+SLG S 
Sbjct: 246 LAGGTVRGGAPRAHIAMYKACWYLDDDDTTTCSSADILKAMDEAMHDGVDVLSISLGSSV 305

Query: 312 PSYSDVDMRNGIAIGAFHAVAKGIVVVGAAGNSGPAAYSVSNIEPWLLTVAASSVDRSFL 371
           P Y + D+R+GI  GAFHAV KGI VV + GNSGP + +V+N  PW++TVAA+++DRSF 
Sbjct: 306 PLYGETDIRDGITTGAFHAVLKGITVVCSGGNSGPDSLTVTNTAPWIITVAATTLDRSFA 365

Query: 372 VAITLGNNWTTMGQGMFSGKLTKFHNLVYPEVSDLDDER---ACESLSLN-DTWAAGNVV 431
             +TLGNN   +GQ M++G    F +LVYPE     +E     CE L  N +    G VV
Sbjct: 366 TPLTLGNNKVILGQAMYTGPGLGFTSLVYPENPGNSNESFSGTCEELLFNSNRTMEGKVV 425

Query: 432 LCFASDDYNDDTHNTSSSVKKVGGLGLIVAKNPTKAVEPFINNFPCVQISLDIGMQILNY 491
           LCF +  Y     + +  VK+ GGLG+I+A++P  A++P +++FPCV +  ++G  IL Y
Sbjct: 426 LCFTTSPYGGAVLSAARYVKRAGGLGVIIARHPGYAIQPCLDDFPCVAVDWELGTDILLY 485

Query: 492 IRSTRNPQVKIGASTTRVGQPLSTTVAYFSSRGPNSVAPAILKPDIAAPGVAILAAVPPS 551
            RS+ +P VKI  S T VGQP+ T VA FSSRGPNS+APAILKPDIAAPGV+ILAA   +
Sbjct: 486 TRSSGSPVVKIQPSKTLVGQPVGTKVATFSSRGPNSIAPAILKPDIAAPGVSILAATTNT 545

Query: 552 DPKDRNSYAFISGTSMATPHVSAIVALLKTLHSHWSPAAIKSAIVTTAWSSDPYGAVIFA 611
              D+  +  +SGTSMA P +S + ALLK LH  WSPAAI+SAIVTTAW +DP+G  IFA
Sbjct: 546 TFSDQ-GFIMLSGTSMAAPAISGVAALLKALHRDWSPAAIRSAIVTTAWKTDPFGEQIFA 605

Query: 612 EGQPMMSARPFDFGGGIVNPNKAVDPGLVYDMGMADYIQYFCAKGYNDSAISGITKRSIS 671
           EG P   A PFD+GGG+VNP K+ +PGLVYDMG+ DY+ Y C+ GYN+++IS +  ++  
Sbjct: 606 EGSPPKLADPFDYGGGLVNPEKSANPGLVYDMGLEDYVLYMCSVGYNETSISQLIGKTTV 665

Query: 672 CPKRRPSILDINVPSITIPSLTHPVSLTRTVTNVGAINSSYKAAIEAPPGITIAIKPRIL 731
           C   +PS+LD N+PSITIP+L   V++TRTVTNVG +NS Y+  +E P G  + + P  L
Sbjct: 666 CSNPKPSVLDFNLPSITIPNLKDEVTITRTVTNVGPLNSVYRVTVEPPLGFQVTVTPETL 725

Query: 732 KFNHKMKSISFTVTISSNHRVTTGYCFGSLTWLDGVHSVRIPVSVRTNI 774
            FN   K + F V +S+ H+  TGY FGSLTW D +H+V IP+SVRT I
Sbjct: 726 VFNSTTKKVYFKVKVSTTHKTNTGYYFGSLTWSDSLHNVTIPLSVRTQI 770

BLAST of Carg15037 vs. ExPASy TrEMBL
Match: A0A6J1E757 (subtilisin-like protease SBT3.9 isoform X1 OS=Cucurbita moschata OX=3662 GN=LOC111430558 PE=3 SV=1)

HSP 1 Score: 1540.8 bits (3988), Expect = 0.0e+00
Identity = 770/773 (99.61%), Postives = 772/773 (99.87%), Query Frame = 0

Query: 1   MGSFAHGKSSSTTIFLVCISVFTALDHLCTLHIRVAAKSNVYIVYMGQKPHDNEELLVKA 60
           MGSFAHGKSSSTTIFLVCISVFTALDHLCTLHIRVAAKSNVYIVYMGQKPHDNEELLVKA
Sbjct: 1   MGSFAHGKSSSTTIFLVCISVFTALDHLCTLHIRVAAKSNVYIVYMGQKPHDNEELLVKA 60

Query: 61  HHGVLASVLGSQEGSVDSLVYSYKYGFSGFAAKLTMAQAQMISELPSVVEVIPNRLHKMQ 120
           HHGVLASVLGSQEGSVDSLVYSYKYGFSGFAAKLTMAQAQMISELPSVVEVIPNRLHKMQ
Sbjct: 61  HHGVLASVLGSQEGSVDSLVYSYKYGFSGFAAKLTMAQAQMISELPSVVEVIPNRLHKMQ 120

Query: 121 TTRSWDYLQLSPRFPNSLLRKSRMGSGAIIGVLDTGIWPESEVFCDEGLGPVPSRWKGIC 180
           TTRSWDYLQLSPRFPNSLLRKSRMGSGAIIGVLDTGIWPESEVFCDEGLGPVPSRWKGIC
Sbjct: 121 TTRSWDYLQLSPRFPNSLLRKSRMGSGAIIGVLDTGIWPESEVFCDEGLGPVPSRWKGIC 180

Query: 181 ESGELFSPAKACSRKLIGARYFVKGLEAAYGHPLNNSEFQDYWSPRDRSGHGTHVSSVAS 240
           ESGELFSPAKACSRKLIGARYFVKGLEAAYGHPLNNSEFQDYWSPRDRSGHGTHVSSVAS
Sbjct: 181 ESGELFSPAKACSRKLIGARYFVKGLEAAYGHPLNNSEFQDYWSPRDRSGHGTHVSSVAS 240

Query: 241 GFFVPNVSYHGLAVGTVRGGAPHSRIAMYKVCWQVNGGVCADIDILKAIDQAIYDGVDVL 300
           GFFVPNVSYHGLAVGTVRGGAPHSRIAMYKVCWQVNGGVCADIDILKAIDQAIYDGVDVL
Sbjct: 241 GFFVPNVSYHGLAVGTVRGGAPHSRIAMYKVCWQVNGGVCADIDILKAIDQAIYDGVDVL 300

Query: 301 SLSLGPSFPSYSDVDMRNGIAIGAFHAVAKGIVVVGAAGNSGPAAYSVSNIEPWLLTVAA 360
           SLSLGPSFPSYSDVDMRNGIAIGAFHAVAKGIVVVGAAGNSGPAAYSVSNIEPWLLTVAA
Sbjct: 301 SLSLGPSFPSYSDVDMRNGIAIGAFHAVAKGIVVVGAAGNSGPAAYSVSNIEPWLLTVAA 360

Query: 361 SSVDRSFLVAITLGNNWTTMGQGMFSGKLTKFHNLVYPEVSDLDDERACESLSLNDTWAA 420
           SSVD SFLVAITLGNNWTTMGQGMFSGKLTKFHNLVYPEVSDLDDERACESLSLNDTWAA
Sbjct: 361 SSVDCSFLVAITLGNNWTTMGQGMFSGKLTKFHNLVYPEVSDLDDERACESLSLNDTWAA 420

Query: 421 GNVVLCFASDDYNDDTHNTSSSVKKVGGLGLIVAKNPTKAVEPFINNFPCVQISLDIGMQ 480
           GNVVLCFASDDYNDDTHNTSSSVKKVGGLGLIVAKNPTKAVEPFINNFPCVQISLDIGMQ
Sbjct: 421 GNVVLCFASDDYNDDTHNTSSSVKKVGGLGLIVAKNPTKAVEPFINNFPCVQISLDIGMQ 480

Query: 481 ILNYIRSTRNPQVKIGASTTRVGQPLSTTVAYFSSRGPNSVAPAILKPDIAAPGVAILAA 540
           ILNYIRSTRNPQVKIGASTTRVGQPLSTTVAYFSSRGPNSVAPAILKPDIAAPGVAILAA
Sbjct: 481 ILNYIRSTRNPQVKIGASTTRVGQPLSTTVAYFSSRGPNSVAPAILKPDIAAPGVAILAA 540

Query: 541 VPPSDPKDRNSYAFISGTSMATPHVSAIVALLKTLHSHWSPAAIKSAIVTTAWSSDPYGA 600
           VPPSDPKDRNSYAFISGTSMATPHVSAIVALLKTLHSHWSPAAIKSAIVTTAWSSDPYGA
Sbjct: 541 VPPSDPKDRNSYAFISGTSMATPHVSAIVALLKTLHSHWSPAAIKSAIVTTAWSSDPYGA 600

Query: 601 VIFAEGQPMMSARPFDFGGGIVNPNKAVDPGLVYDMGMADYIQYFCAKGYNDSAISGITK 660
           VIFAEGQPMMSARPFDFGGGIVNPNKAVDPGLVYDMGMADYIQYFCAKGYN+SAISGITK
Sbjct: 601 VIFAEGQPMMSARPFDFGGGIVNPNKAVDPGLVYDMGMADYIQYFCAKGYNNSAISGITK 660

Query: 661 RSISCPKRRPSILDINVPSITIPSLTHPVSLTRTVTNVGAINSSYKAAIEAPPGITIAIK 720
           RSISCPKRRPSILDINVPSITIPSLTHPVSLTRTVTNVGAINSSYKAAIEAPPGITIA+K
Sbjct: 661 RSISCPKRRPSILDINVPSITIPSLTHPVSLTRTVTNVGAINSSYKAAIEAPPGITIAVK 720

Query: 721 PRILKFNHKMKSISFTVTISSNHRVTTGYCFGSLTWLDGVHSVRIPVSVRTNI 774
           PRILKFNHKMKSISFTVTISSNHRVTTGYCFGSLTWLDGVHSVRIPVSVRTNI
Sbjct: 721 PRILKFNHKMKSISFTVTISSNHRVTTGYCFGSLTWLDGVHSVRIPVSVRTNI 773

BLAST of Carg15037 vs. ExPASy TrEMBL
Match: A0A6J1J508 (subtilisin-like protease SBT3.9 isoform X1 OS=Cucurbita maxima OX=3661 GN=LOC111482683 PE=3 SV=1)

HSP 1 Score: 1510.4 bits (3909), Expect = 0.0e+00
Identity = 757/773 (97.93%), Postives = 766/773 (99.09%), Query Frame = 0

Query: 1   MGSFAHGKSSSTTIFLVCISVFTALDHLCTLHIRVAAKSNVYIVYMGQKPHDNEELLVKA 60
           MGSFAHGKSSSTTIFLVCISVFTALDHLCTLHIRVAAKSNVYIVYMGQKPHDNEELLVKA
Sbjct: 1   MGSFAHGKSSSTTIFLVCISVFTALDHLCTLHIRVAAKSNVYIVYMGQKPHDNEELLVKA 60

Query: 61  HHGVLASVLGSQEGSVDSLVYSYKYGFSGFAAKLTMAQAQMISELPSVVEVIPNRLHKMQ 120
           HHGVLASVLGSQEGSVDSLVYSYKYGFSGFAAKLTMAQAQMISELPSVVEVIPNRLHK+Q
Sbjct: 61  HHGVLASVLGSQEGSVDSLVYSYKYGFSGFAAKLTMAQAQMISELPSVVEVIPNRLHKIQ 120

Query: 121 TTRSWDYLQLSPRFPNSLLRKSRMGSGAIIGVLDTGIWPESEVFCDEGLGPVPSRWKGIC 180
           TTRSWDYLQLSPRFPNSLLRKSRMGSGAIIGVLDTGIWPESEVFCDEGLGPVPSRWKGIC
Sbjct: 121 TTRSWDYLQLSPRFPNSLLRKSRMGSGAIIGVLDTGIWPESEVFCDEGLGPVPSRWKGIC 180

Query: 181 ESGELFSPAKACSRKLIGARYFVKGLEAAYGHPLNNSEFQDYWSPRDRSGHGTHVSSVAS 240
           ESGELFSPAKACSRKLIGARYFVKGLEAAYGHPLNNSEFQDY SPRDRSGHGTHVSSVAS
Sbjct: 181 ESGELFSPAKACSRKLIGARYFVKGLEAAYGHPLNNSEFQDYLSPRDRSGHGTHVSSVAS 240

Query: 241 GFFVPNVSYHGLAVGTVRGGAPHSRIAMYKVCWQVNGGVCADIDILKAIDQAIYDGVDVL 300
           GFFVPNVSYHGLAVGTVRGGAPHSRIAMYKVCWQVNGGVCADIDILKAIDQAIYDGVDVL
Sbjct: 241 GFFVPNVSYHGLAVGTVRGGAPHSRIAMYKVCWQVNGGVCADIDILKAIDQAIYDGVDVL 300

Query: 301 SLSLGPSFPSYSDVDMRNGIAIGAFHAVAKGIVVVGAAGNSGPAAYSVSNIEPWLLTVAA 360
           SLSLGPSFPSYSDVDMRNGIAIGAFHAVAKGIVVVGAAGNSGPAAYSVSNIEPWLLTVAA
Sbjct: 301 SLSLGPSFPSYSDVDMRNGIAIGAFHAVAKGIVVVGAAGNSGPAAYSVSNIEPWLLTVAA 360

Query: 361 SSVDRSFLVAITLGNNWTTMGQGMFSGKLTKFHNLVYPEVSDLDDERACESLSLNDTWAA 420
           SSVDRSFLVAITLGNNWTTMGQGMFSGKLTKF NLVYPEVSDLDDERACESLSLNDTWA+
Sbjct: 361 SSVDRSFLVAITLGNNWTTMGQGMFSGKLTKFINLVYPEVSDLDDERACESLSLNDTWAS 420

Query: 421 GNVVLCFASDDYNDDTHNTSSSVKKVGGLGLIVAKNPTKAVEPFINNFPCVQISLDIGMQ 480
           GNVVLCFASDDYND+THNTS SVKKVGGLGLIVAKNPTKAVEPFI+NFPCVQISLDIGMQ
Sbjct: 421 GNVVLCFASDDYNDNTHNTSWSVKKVGGLGLIVAKNPTKAVEPFIDNFPCVQISLDIGMQ 480

Query: 481 ILNYIRSTRNPQVKIGASTTRVGQPLSTTVAYFSSRGPNSVAPAILKPDIAAPGVAILAA 540
           ILNYIRSTRNPQVKIGASTTRVGQPLSTTVAYFSSRGPNSVAPAILKPDIAAPGVAILAA
Sbjct: 481 ILNYIRSTRNPQVKIGASTTRVGQPLSTTVAYFSSRGPNSVAPAILKPDIAAPGVAILAA 540

Query: 541 VPPSDPKDRNSYAFISGTSMATPHVSAIVALLKTLHSHWSPAAIKSAIVTTAWSSDPYGA 600
           VPPSDPK+RNSYAFISGTSMATPHVSAIVALLKTLHSHWSPAAIKSAIVTTAWSSDPYGA
Sbjct: 541 VPPSDPKNRNSYAFISGTSMATPHVSAIVALLKTLHSHWSPAAIKSAIVTTAWSSDPYGA 600

Query: 601 VIFAEGQPMMSARPFDFGGGIVNPNKAVDPGLVYDMGMADYIQYFCAKGYNDSAISGITK 660
           VIFAEG+PMMSARPFDFGGGIVNPNKAVDPGLVYDMGMADYIQYFCAKGYN+SAISGI+K
Sbjct: 601 VIFAEGRPMMSARPFDFGGGIVNPNKAVDPGLVYDMGMADYIQYFCAKGYNNSAISGISK 660

Query: 661 RSISCPKRRPSILDINVPSITIPSLTHPVSLTRTVTNVGAINSSYKAAIEAPPGITIAIK 720
           RSISCPKRRPSILDINVPSITIPSLTH VSLTRTVTNVGAINSSYKAAIE PPGITIA+K
Sbjct: 661 RSISCPKRRPSILDINVPSITIPSLTHSVSLTRTVTNVGAINSSYKAAIEVPPGITIAVK 720

Query: 721 PRILKFNHKMKSISFTVTISSNHRVTTGYCFGSLTWLDGVHSVRIPVSVRTNI 774
           PRILKFNHK KSISFTVTISSN RVTTGYCFGSLTWLDGVHSVRIPVSVRTNI
Sbjct: 721 PRILKFNHKTKSISFTVTISSNQRVTTGYCFGSLTWLDGVHSVRIPVSVRTNI 773

BLAST of Carg15037 vs. ExPASy TrEMBL
Match: A0A6J1CIU2 (subtilisin-like protease SBT3.9 OS=Momordica charantia OX=3673 GN=LOC111011560 PE=3 SV=1)

HSP 1 Score: 1354.0 bits (3503), Expect = 0.0e+00
Identity = 668/775 (86.19%), Postives = 718/775 (92.65%), Query Frame = 0

Query: 1   MGSFAHGKSSSTTIFLVCISVFTALDHLCTLHIRVAAKSNVYIVYMGQKPHDNEELLVKA 60
           M SFAHGKSSSTTI L+ IS FT L  LCTL + V AKS+VY+VYMG++PHDNEELLVK 
Sbjct: 1   MSSFAHGKSSSTTILLLFISFFTLLSDLCTLLVPVDAKSHVYVVYMGKRPHDNEELLVKT 60

Query: 61  HHGVLASVLGSQEGSVDSLVYSYKYGFSGFAAKLTMAQAQMISELPSVVEVIPNRLHKMQ 120
           HHGVLASVLGSQE   DS+VY+YKYGFSGFAAKLTMAQAQ ISELP+V++VIPNRLHKMQ
Sbjct: 61  HHGVLASVLGSQEAPADSMVYNYKYGFSGFAAKLTMAQAQKISELPTVIKVIPNRLHKMQ 120

Query: 121 TTRSWDYLQLSPRFPNSLLRKSRMGSGAIIGVLDTGIWPESEVFCDEGLGPVPSRWKGIC 180
           TTRSWDYLQLSPR  NSLL+KSRMG+GAIIG+LDTGIWPESEVF D GLGPVPSRWKGIC
Sbjct: 121 TTRSWDYLQLSPRLQNSLLQKSRMGNGAIIGLLDTGIWPESEVFSDNGLGPVPSRWKGIC 180

Query: 181 ESGELFSPAKACSRKLIGARYFVKGLEAAYGHPLNNSEFQDYWSPRDRSGHGTHVSSVAS 240
           ESGELFSP KACSRKLIGARYF+KGLEAAYGHP NNS FQDY SPRD +GHGTHVSSVAS
Sbjct: 181 ESGELFSPGKACSRKLIGARYFIKGLEAAYGHPYNNSGFQDYLSPRDYAGHGTHVSSVAS 240

Query: 241 GFFVPNVSYHGLAVGTVRGGAPHSRIAMYKVCWQVNGGVCADIDILKAIDQAIYDGVDVL 300
           G FV NVSYHGLAVGTVRGGAP SR+AMYKVCWQ+NGGVC+D DILKAIDQAI+DGVDVL
Sbjct: 241 GSFVANVSYHGLAVGTVRGGAPQSRLAMYKVCWQLNGGVCSDADILKAIDQAIHDGVDVL 300

Query: 301 SLSLGPSFPSYSDVDMRNGIAIGAFHAVAKGIVVVGAAGNSGPAAYSVSNIEPWLLTVAA 360
           SLSLGP+FPSYSDVDM NG+AIG FHA+ KGIVVVGAAGN GPAAYSV+NIEPWLLTVAA
Sbjct: 301 SLSLGPTFPSYSDVDMHNGVAIGTFHAIVKGIVVVGAAGNYGPAAYSVANIEPWLLTVAA 360

Query: 361 SSVDRSFLVAITLGNNWTTMGQGMFSGKLTKFHNLVYPEVSDLDDERACESLSLNDTWAA 420
           SSVDRSFLVAITLGNNWTT+GQGMFSGKL +FHNLVYPEVSDL+DERACESLSLNDTWAA
Sbjct: 361 SSVDRSFLVAITLGNNWTTVGQGMFSGKLRRFHNLVYPEVSDLEDERACESLSLNDTWAA 420

Query: 421 GNVVLCFASDDYNDDTHNTSSSVKKVGGLGLIVAKNPTKAVEPFINNFPCVQISLDIGMQ 480
           GNVVLCFASDD+ND+TH+TS SVKKVG LGLIVAKNPTK VEPFIN+FPCVQ+SL+IGM 
Sbjct: 421 GNVVLCFASDDFNDNTHHTSRSVKKVGALGLIVAKNPTKDVEPFINHFPCVQVSLEIGMH 480

Query: 481 ILNYIRSTRNPQVKIGASTTRVGQPLSTTVAYFSSRGPNSVAPAILKPDIAAPGVAILAA 540
           ILNYIRSTRNPQV IG S TR+GQ LSTTVAYFSSRGPNSVAPAILKPDIAAPGVAILAA
Sbjct: 481 ILNYIRSTRNPQVNIGPSRTRIGQSLSTTVAYFSSRGPNSVAPAILKPDIAAPGVAILAA 540

Query: 541 VPPSDPKDRNSYAFISGTSMATPHVSAIVALLKTLHSHWSPAAIKSAIVTTAWSSDPYGA 600
           VPPSDPKD+NSYAFISGTSMATPHVSAIVALLK+LHSHWSPAAIKSAIVTTAW+SDPYG 
Sbjct: 541 VPPSDPKDKNSYAFISGTSMATPHVSAIVALLKSLHSHWSPAAIKSAIVTTAWTSDPYGE 600

Query: 601 VIFAEGQPMMSARPFDFGGGIVNPNKAVDPGLVYDMGMADYIQYFCAKGYNDSAISGITK 660
            IFAEG+P+ SA PFDFGGGIVNPNKAVDPGLVYDMGMADYI+YFCA GYNDSAISGITK
Sbjct: 601 TIFAEGRPIKSAGPFDFGGGIVNPNKAVDPGLVYDMGMADYIRYFCAMGYNDSAISGITK 660

Query: 661 RSISCPKRRPSILDINVPSITIPSLTHPVSLTRTVTNVGAINSSYKAAIEAP--PGITIA 720
           + ISCPKRRPSILD NVPSITIP L H V++TRTVTNVGA+NS+YKAAIE P  PGI IA
Sbjct: 661 KPISCPKRRPSILDTNVPSITIPGLKHSVTVTRTVTNVGAVNSTYKAAIETPPGPGIAIA 720

Query: 721 IKPRILKFNHKMKSISFTVTISSNHRVTTGYCFGSLTWLDGVHSVRIPVSVRTNI 774
           +KPR+LKFNHK K ISFTVT+SSNHRVTTGYCFGSLTWLDGVHSVRIP+SVRT+I
Sbjct: 721 VKPRMLKFNHKRKIISFTVTVSSNHRVTTGYCFGSLTWLDGVHSVRIPISVRTDI 775

BLAST of Carg15037 vs. ExPASy TrEMBL
Match: A0A6J1E3V3 (subtilisin-like protease SBT3.3 isoform X2 OS=Cucurbita moschata OX=3662 GN=LOC111430558 PE=3 SV=1)

HSP 1 Score: 1312.4 bits (3395), Expect = 0.0e+00
Identity = 652/655 (99.54%), Postives = 654/655 (99.85%), Query Frame = 0

Query: 119 MQTTRSWDYLQLSPRFPNSLLRKSRMGSGAIIGVLDTGIWPESEVFCDEGLGPVPSRWKG 178
           MQTTRSWDYLQLSPRFPNSLLRKSRMGSGAIIGVLDTGIWPESEVFCDEGLGPVPSRWKG
Sbjct: 1   MQTTRSWDYLQLSPRFPNSLLRKSRMGSGAIIGVLDTGIWPESEVFCDEGLGPVPSRWKG 60

Query: 179 ICESGELFSPAKACSRKLIGARYFVKGLEAAYGHPLNNSEFQDYWSPRDRSGHGTHVSSV 238
           ICESGELFSPAKACSRKLIGARYFVKGLEAAYGHPLNNSEFQDYWSPRDRSGHGTHVSSV
Sbjct: 61  ICESGELFSPAKACSRKLIGARYFVKGLEAAYGHPLNNSEFQDYWSPRDRSGHGTHVSSV 120

Query: 239 ASGFFVPNVSYHGLAVGTVRGGAPHSRIAMYKVCWQVNGGVCADIDILKAIDQAIYDGVD 298
           ASGFFVPNVSYHGLAVGTVRGGAPHSRIAMYKVCWQVNGGVCADIDILKAIDQAIYDGVD
Sbjct: 121 ASGFFVPNVSYHGLAVGTVRGGAPHSRIAMYKVCWQVNGGVCADIDILKAIDQAIYDGVD 180

Query: 299 VLSLSLGPSFPSYSDVDMRNGIAIGAFHAVAKGIVVVGAAGNSGPAAYSVSNIEPWLLTV 358
           VLSLSLGPSFPSYSDVDMRNGIAIGAFHAVAKGIVVVGAAGNSGPAAYSVSNIEPWLLTV
Sbjct: 181 VLSLSLGPSFPSYSDVDMRNGIAIGAFHAVAKGIVVVGAAGNSGPAAYSVSNIEPWLLTV 240

Query: 359 AASSVDRSFLVAITLGNNWTTMGQGMFSGKLTKFHNLVYPEVSDLDDERACESLSLNDTW 418
           AASSVD SFLVAITLGNNWTTMGQGMFSGKLTKFHNLVYPEVSDLDDERACESLSLNDTW
Sbjct: 241 AASSVDCSFLVAITLGNNWTTMGQGMFSGKLTKFHNLVYPEVSDLDDERACESLSLNDTW 300

Query: 419 AAGNVVLCFASDDYNDDTHNTSSSVKKVGGLGLIVAKNPTKAVEPFINNFPCVQISLDIG 478
           AAGNVVLCFASDDYNDDTHNTSSSVKKVGGLGLIVAKNPTKAVEPFINNFPCVQISLDIG
Sbjct: 301 AAGNVVLCFASDDYNDDTHNTSSSVKKVGGLGLIVAKNPTKAVEPFINNFPCVQISLDIG 360

Query: 479 MQILNYIRSTRNPQVKIGASTTRVGQPLSTTVAYFSSRGPNSVAPAILKPDIAAPGVAIL 538
           MQILNYIRSTRNPQVKIGASTTRVGQPLSTTVAYFSSRGPNSVAPAILKPDIAAPGVAIL
Sbjct: 361 MQILNYIRSTRNPQVKIGASTTRVGQPLSTTVAYFSSRGPNSVAPAILKPDIAAPGVAIL 420

Query: 539 AAVPPSDPKDRNSYAFISGTSMATPHVSAIVALLKTLHSHWSPAAIKSAIVTTAWSSDPY 598
           AAVPPSDPKDRNSYAFISGTSMATPHVSAIVALLKTLHSHWSPAAIKSAIVTTAWSSDPY
Sbjct: 421 AAVPPSDPKDRNSYAFISGTSMATPHVSAIVALLKTLHSHWSPAAIKSAIVTTAWSSDPY 480

Query: 599 GAVIFAEGQPMMSARPFDFGGGIVNPNKAVDPGLVYDMGMADYIQYFCAKGYNDSAISGI 658
           GAVIFAEGQPMMSARPFDFGGGIVNPNKAVDPGLVYDMGMADYIQYFCAKGYN+SAISGI
Sbjct: 481 GAVIFAEGQPMMSARPFDFGGGIVNPNKAVDPGLVYDMGMADYIQYFCAKGYNNSAISGI 540

Query: 659 TKRSISCPKRRPSILDINVPSITIPSLTHPVSLTRTVTNVGAINSSYKAAIEAPPGITIA 718
           TKRSISCPKRRPSILDINVPSITIPSLTHPVSLTRTVTNVGAINSSYKAAIEAPPGITIA
Sbjct: 541 TKRSISCPKRRPSILDINVPSITIPSLTHPVSLTRTVTNVGAINSSYKAAIEAPPGITIA 600

Query: 719 IKPRILKFNHKMKSISFTVTISSNHRVTTGYCFGSLTWLDGVHSVRIPVSVRTNI 774
           +KPRILKFNHKMKSISFTVTISSNHRVTTGYCFGSLTWLDGVHSVRIPVSVRTNI
Sbjct: 601 VKPRILKFNHKMKSISFTVTISSNHRVTTGYCFGSLTWLDGVHSVRIPVSVRTNI 655

BLAST of Carg15037 vs. ExPASy TrEMBL
Match: A0A6J1J1Z1 (subtilisin-like protease SBT3.3 isoform X2 OS=Cucurbita maxima OX=3661 GN=LOC111482683 PE=3 SV=1)

HSP 1 Score: 1231.9 bits (3186), Expect = 0.0e+00
Identity = 615/630 (97.62%), Postives = 623/630 (98.89%), Query Frame = 0

Query: 144 MGSGAIIGVLDTGIWPESEVFCDEGLGPVPSRWKGICESGELFSPAKACSRKLIGARYFV 203
           MGSGAIIGVLDTGIWPESEVFCDEGLGPVPSRWKGICESGELFSPAKACSRKLIGARYFV
Sbjct: 1   MGSGAIIGVLDTGIWPESEVFCDEGLGPVPSRWKGICESGELFSPAKACSRKLIGARYFV 60

Query: 204 KGLEAAYGHPLNNSEFQDYWSPRDRSGHGTHVSSVASGFFVPNVSYHGLAVGTVRGGAPH 263
           KGLEAAYGHPLNNSEFQDY SPRDRSGHGTHVSSVASGFFVPNVSYHGLAVGTVRGGAPH
Sbjct: 61  KGLEAAYGHPLNNSEFQDYLSPRDRSGHGTHVSSVASGFFVPNVSYHGLAVGTVRGGAPH 120

Query: 264 SRIAMYKVCWQVNGGVCADIDILKAIDQAIYDGVDVLSLSLGPSFPSYSDVDMRNGIAIG 323
           SRIAMYKVCWQVNGGVCADIDILKAIDQAIYDGVDVLSLSLGPSFPSYSDVDMRNGIAIG
Sbjct: 121 SRIAMYKVCWQVNGGVCADIDILKAIDQAIYDGVDVLSLSLGPSFPSYSDVDMRNGIAIG 180

Query: 324 AFHAVAKGIVVVGAAGNSGPAAYSVSNIEPWLLTVAASSVDRSFLVAITLGNNWTTMGQG 383
           AFHAVAKGIVVVGAAGNSGPAAYSVSNIEPWLLTVAASSVDRSFLVAITLGNNWTTMGQG
Sbjct: 181 AFHAVAKGIVVVGAAGNSGPAAYSVSNIEPWLLTVAASSVDRSFLVAITLGNNWTTMGQG 240

Query: 384 MFSGKLTKFHNLVYPEVSDLDDERACESLSLNDTWAAGNVVLCFASDDYNDDTHNTSSSV 443
           MFSGKLTKF NLVYPEVSDLDDERACESLSLNDTWA+GNVVLCFASDDYND+THNTS SV
Sbjct: 241 MFSGKLTKFINLVYPEVSDLDDERACESLSLNDTWASGNVVLCFASDDYNDNTHNTSWSV 300

Query: 444 KKVGGLGLIVAKNPTKAVEPFINNFPCVQISLDIGMQILNYIRSTRNPQVKIGASTTRVG 503
           KKVGGLGLIVAKNPTKAVEPFI+NFPCVQISLDIGMQILNYIRSTRNPQVKIGASTTRVG
Sbjct: 301 KKVGGLGLIVAKNPTKAVEPFIDNFPCVQISLDIGMQILNYIRSTRNPQVKIGASTTRVG 360

Query: 504 QPLSTTVAYFSSRGPNSVAPAILKPDIAAPGVAILAAVPPSDPKDRNSYAFISGTSMATP 563
           QPLSTTVAYFSSRGPNSVAPAILKPDIAAPGVAILAAVPPSDPK+RNSYAFISGTSMATP
Sbjct: 361 QPLSTTVAYFSSRGPNSVAPAILKPDIAAPGVAILAAVPPSDPKNRNSYAFISGTSMATP 420

Query: 564 HVSAIVALLKTLHSHWSPAAIKSAIVTTAWSSDPYGAVIFAEGQPMMSARPFDFGGGIVN 623
           HVSAIVALLKTLHSHWSPAAIKSAIVTTAWSSDPYGAVIFAEG+PMMSARPFDFGGGIVN
Sbjct: 421 HVSAIVALLKTLHSHWSPAAIKSAIVTTAWSSDPYGAVIFAEGRPMMSARPFDFGGGIVN 480

Query: 624 PNKAVDPGLVYDMGMADYIQYFCAKGYNDSAISGITKRSISCPKRRPSILDINVPSITIP 683
           PNKAVDPGLVYDMGMADYIQYFCAKGYN+SAISGI+KRSISCPKRRPSILDINVPSITIP
Sbjct: 481 PNKAVDPGLVYDMGMADYIQYFCAKGYNNSAISGISKRSISCPKRRPSILDINVPSITIP 540

Query: 684 SLTHPVSLTRTVTNVGAINSSYKAAIEAPPGITIAIKPRILKFNHKMKSISFTVTISSNH 743
           SLTH VSLTRTVTNVGAINSSYKAAIE PPGITIA+KPRILKFNHK KSISFTVTISSN 
Sbjct: 541 SLTHSVSLTRTVTNVGAINSSYKAAIEVPPGITIAVKPRILKFNHKTKSISFTVTISSNQ 600

Query: 744 RVTTGYCFGSLTWLDGVHSVRIPVSVRTNI 774
           RVTTGYCFGSLTWLDGVHSVRIPVSVRTNI
Sbjct: 601 RVTTGYCFGSLTWLDGVHSVRIPVSVRTNI 630

BLAST of Carg15037 vs. TAIR 10
Match: AT1G32960.1 (Subtilase family protein )

HSP 1 Score: 823.9 bits (2127), Expect = 1.1e-238
Identity = 422/773 (54.59%), Postives = 548/773 (70.89%), Query Frame = 0

Query: 8   KSSSTTIFLVCISVFTALDHLCTLHIRVAAKSNVYIVYMGQKPHDNEELLVKAHHGVLAS 67
           +S  ++I LV +S+ T L+       R   +S V+IVY+G+K H + E + ++HH +LAS
Sbjct: 2   RSFRSSILLVLLSLITVLN---ATRARSETESKVHIVYLGEKKHHDPEFVTESHHQMLAS 61

Query: 68  VLGSQEGSVDSLVYSYKYGFSGFAAKLTMAQAQMISELPSVVEVIPNRLHKMQTTRSWDY 127
           +LGS++ + DS+VYSY++GFSGFAAKLT +QA+ I++LP VV VIP+  H++ TTR+W+Y
Sbjct: 62  LLGSKKDADDSMVYSYRHGFSGFAAKLTKSQAKKIADLPEVVHVIPDGFHELATTRTWEY 121

Query: 128 LQLSPRFPNSLLRKSRMGSGAIIGVLDTGIWPESEVFCDEGLGPVPSRWKGICESGELFS 187
           L LS   P +LL  + MG   IIGV+DTG+WPESE F D G+GP+P +WKG CESGE F 
Sbjct: 122 LGLSSANPKNLLNDTNMGDQVIIGVIDTGVWPESESFNDNGVGPIPRKWKGGCESGENFR 181

Query: 188 PAKACSRKLIGARYFVKGLEAAYGHPLNNSEFQDYWSPRDRSGHGTHVSSVASGFFVPNV 247
            +  C+RKLIGA+YF+ G   A     N +E +DY S RD  GHGTHV+S+A G FVPNV
Sbjct: 182 -STDCNRKLIGAKYFINGF-LAENKGFNTTESRDYISARDFDGHGTHVASIAGGSFVPNV 241

Query: 248 SYHGLAVGTVRGGAPHSRIAMYKVCW---QVNGGVCADIDILKAIDQAIYDGVDVLSLSL 307
           SY GLA GT+RGGAP +RIAMYK CW   ++ G  C+D DI+KAID+AI+DGVDVLS+SL
Sbjct: 242 SYKGLAGGTLRGGAPRARIAMYKACWFHEELKGVTCSDSDIMKAIDEAIHDGVDVLSISL 301

Query: 308 GPSFPSYSDVDMRNGIAIGAFHAVAKGIVVVGAAGNSGPAAYSVSNIEPWLLTVAASSVD 367
               P  S+ D+R+  A G FHAVAKGIVVV A GN GPAA +V NI PW+LTVAA+++D
Sbjct: 302 VGQIPLNSETDIRDEFATGLFHAVAKGIVVVCAGGNDGPAAQTVVNIAPWILTVAATTLD 361

Query: 368 RSFLVAITLGNNWTTMGQGMFSGKLTKFHNLVYPEVSDLDDER---ACESLSLNDTWA-A 427
           RSF   ITLGNN   +GQ  ++G      +LVYPE +  ++E     CESL+LN  +  A
Sbjct: 362 RSFPTPITLGNNKVILGQATYTGPELGLTSLVYPENARNNNETFSGVCESLNLNPNYTMA 421

Query: 428 GNVVLCFASDDYNDDTHNTSSSVKKVGGLGLIVAKNPTKAVEPFINNFPCVQISLDIGMQ 487
             VVLCF +   N      +S VK  GGLGLI+++NP   + P  ++FPCV +  ++G  
Sbjct: 422 MKVVLCFTASRTNAAISRAASFVKAAGGLGLIISRNPVYTLSPCNDDFPCVAVDYELGTD 481

Query: 488 ILNYIRSTRNPQVKIGASTTRVGQPLSTTVAYFSSRGPNSVAPAILKPDIAAPGVAILAA 547
           IL+YIRSTR+P VKI  S T  GQP+ T V  FSSRGPNS++PAILKPDIAAPGV ILAA
Sbjct: 482 ILSYIRSTRSPVVKIQRSRTLSGQPVGTKVVNFSSRGPNSMSPAILKPDIAAPGVRILAA 541

Query: 548 VPPSDPKDRNSYAFISGTSMATPHVSAIVALLKTLHSHWSPAAIKSAIVTTAWSSDPYGA 607
             P+D  +   +A +SGTSMATP +S ++ALLK LH  WSPAA +SAIVTTAW +DP+G 
Sbjct: 542 TSPNDTLNVGGFAMLSGTSMATPVISGVIALLKALHPEWSPAAFRSAIVTTAWRTDPFGE 601

Query: 608 VIFAEGQPMMSARPFDFGGGIVNPNKAVDPGLVYDMGMADYIQYFCAKGYNDSAISGITK 667
            IFAEG     + PFD+GGGIVNP KA +PGL+YDMG  DYI Y C+ GYNDS+IS +  
Sbjct: 602 QIFAEGSSRKVSDPFDYGGGIVNPEKAAEPGLIYDMGPQDYILYLCSAGYNDSSISQLVG 661

Query: 668 RSISCPKRRPSILDINVPSITIPSLTHPVSLTRTVTNVGAINSSYKAAIEAPPGITIAIK 727
           +   C   +PS+LD+N+PSITIP+L   V+LTRTVTNVG ++S YK ++E P G+ + + 
Sbjct: 662 QITVCSNPKPSVLDVNLPSITIPNLKDEVTLTRTVTNVGLVDSVYKVSVEPPLGVRVVVT 721

Query: 728 PRILKFNHKMKSISFTVTISSNHRVTTGYCFGSLTWLDGVHSVRIPVSVRTNI 774
           P  L FN K  S+SFTV +S+ H++ TGY FGSLTW D VH+V IP+SVRT I
Sbjct: 722 PETLVFNSKTISVSFTVRVSTTHKINTGYYFGSLTWTDSVHNVVIPLSVRTQI 769

BLAST of Carg15037 vs. TAIR 10
Match: AT4G10520.1 (Subtilase family protein )

HSP 1 Score: 815.5 bits (2105), Expect = 3.7e-236
Identity = 422/740 (57.03%), Postives = 540/740 (72.97%), Query Frame = 0

Query: 35  VAAKSNVYIVYMGQKPHDNEELLVKAHHGVLASVLGSQEGSVDSLVYSYKYGFSGFAAKL 94
           V A+S VY+VY+G+K HDN E + ++HH +L S+LGS+E  +DS+VYSY++GFSGFAAKL
Sbjct: 23  VVAESKVYVVYLGEKEHDNPESVTESHHQMLWSLLGSKEAVLDSIVYSYRHGFSGFAAKL 82

Query: 95  TMAQAQMISELPSVVEVIPNRLHKMQTTRSWDYLQLSPRFPNSLLRKSRMGSGAIIGVLD 154
           T +QAQ ISELP VV+VIPN L++M TTR+WDYL +SP   +SLL+K+ MG   I+GV+D
Sbjct: 83  TESQAQQISELPEVVQVIPNTLYEMTTTRTWDYLGVSPGNSDSLLQKANMGYNVIVGVID 142

Query: 155 TGIWPESEVFCDEGLGPVPSRWKGICESGELFSPAKACSRKLIGARYFVKGLEAAYGHPL 214
           +G+WPESE+F D+G GP+PSRWKG CESGELF+ +  C+RKLIGA+YFV GL A +G  +
Sbjct: 143 SGVWPESEMFNDKGFGPIPSRWKGGCESGELFNASIHCNRKLIGAKYFVDGLVAEFG-VV 202

Query: 215 NNSEFQDYWSPRDRSGHGTHVSSVASGFFVPNVSYHGLAVGTVRGGAPHSRIAMYKVCWQ 274
           N ++  +Y SPRD +GHGTHV+S   G F+PNVSY GL  GT RGGAP   IA+YK CW 
Sbjct: 203 NRTQNPEYLSPRDFAGHGTHVASTIGGSFLPNVSYVGLGRGTARGGAPGVHIAVYKACW- 262

Query: 275 VNGGVCADIDILKAIDQAIYDGVDVLSLSLGPSFPSYSDVDMRNGIAIGAFHAVAKGIVV 334
              G C+  D+LKA+D+AI+DGVD+LSLSLGPS P + + +     ++GAFHAVAKGI V
Sbjct: 263 --SGYCSGADVLKAMDEAIHDGVDILSLSLGPSVPLFPETE---HTSVGAFHAVAKGIPV 322

Query: 335 VGAAGNSGPAAYSVSNIEPWLLTVAASSVDRSFLVAITLGNNWTTMGQGMFSGKLTKFHN 394
           V AAGN+GP A ++SN+ PW+LTVAA++ DRSF  AITLGNN T +GQ ++ G    F  
Sbjct: 323 VIAAGNAGPTAQTISNVAPWVLTVAATTQDRSFPTAITLGNNITILGQAIYGGPELGFVG 382

Query: 395 LVYPEVSDLDDERACESLSLN-DTWAAGNVVLCFASDDYNDDTHNTSSSVKKVGGLGLIV 454
           L YPE S L  +  CE LS N ++   G VVLCFA+   +  ++   ++V   GGLGLI+
Sbjct: 383 LTYPE-SPLSGD--CEKLSANPNSTMEGKVVLCFAA---STPSNAAIAAVINAGGLGLIM 442

Query: 455 AKNPTKAVEPFINNFPCVQISLDIGMQILNYIRSTRNPQVKIGASTTRVGQPLSTTVAYF 514
           AKNPT ++ P    FP V I  ++G  IL YIRSTR+P VKI AS T  GQ +ST VA F
Sbjct: 443 AKNPTHSLTP-TRKFPWVSIDFELGTDILFYIRSTRSPIVKIQASKTLFGQSVSTKVATF 502

Query: 515 SSRGPNSVAPAILKPDIAAPGVAILAAVPPSDPKDRNSYAFISGTSMATPHVSAIVALLK 574
           SSRGPNSV+PAILKPDIAAPGV ILAA+ P+   +   +A +SGTSMATP VS +V LLK
Sbjct: 503 SSRGPNSVSPAILKPDIAAPGVNILAAISPNSSINDGGFAMMSGTSMATPVVSGVVVLLK 562

Query: 575 TLHSHWSPAAIKSAIVTTAWSSDPYGAVIFAEGQPMMSARPFDFGGGIVNPNKAVDPGLV 634
           +LH  WSP+AIKSAIVTTAW +DP G  IFA+G     A PFD+GGG++NP KAV PGL+
Sbjct: 563 SLHPDWSPSAIKSAIVTTAWRTDPSGEPIFADGSSRKLADPFDYGGGLINPEKAVKPGLI 622

Query: 635 YDMGMADYIQYFCAKGYNDSAISGITKRSISCPKRRPSILDINVPSITIPSLTHPVSLTR 694
           YDM   DY+ Y C+  Y+D +IS +  +   CP  +PS+LD+N+PSITIP+L   V+LTR
Sbjct: 623 YDMTTDDYVMYMCSVDYSDISISRVLGKITVCPNPKPSVLDLNLPSITIPNLRGEVTLTR 682

Query: 695 TVTNVGAINSSYKAAIEAPPGITIAIKPRILKFNHKMKSISFTVTISSNHRVTTGYCFGS 754
           TVTNVG +NS YK  I+ P GI +A+ P  L F++     SFTV +S+ H+V TGY FGS
Sbjct: 683 TVTNVGPVNSVYKVVIDPPTGINVAVTPAELVFDYTTTKRSFTVRVSTTHKVNTGYYFGS 742

Query: 755 LTWLDGVHSVRIPVSVRTNI 774
           LTW D +H+V IPVSVRT I
Sbjct: 743 LTWTDNMHNVAIPVSVRTQI 748

BLAST of Carg15037 vs. TAIR 10
Match: AT4G10550.3 (Subtilase family protein )

HSP 1 Score: 806.6 bits (2082), Expect = 1.7e-233
Identity = 410/773 (53.04%), Postives = 539/773 (69.73%), Query Frame = 0

Query: 8   KSSSTTIFLVCISVFTALDHLCTLHIRVAAKSNVYIVYMGQKPHDNEELLVKAHHGVLAS 67
           KSS  TIFL     F A           +AK  V+IVY+G+K HD+ E + ++HH +L S
Sbjct: 26  KSSKETIFLTKERSFVA---------ESSAKRKVHIVYLGEKQHDDPEFVTESHHRMLWS 85

Query: 68  VLGSQEGSVDSLVYSYKYGFSGFAAKLTMAQAQMISELPSVVEVIPNRLHKMQTTRSWDY 127
           +LGS+E + DS+VYSY++GFSGFAAKLT +QA+ I++LP VV VIP+  +K+ TTR+WDY
Sbjct: 86  LLGSKEDANDSMVYSYRHGFSGFAAKLTESQAKKIADLPDVVHVIPDSFYKLATTRTWDY 145

Query: 128 LQLSPRFPNSLLRKSRMGSGAIIGVLDTGIWPESEVFCDEGLGPVPSRWKGICESGELFS 187
           L LS   P SLL ++ MG   IIGV+DTG+WPESEVF D G GPVPS WKG CE+GE F+
Sbjct: 146 LGLSAANPKSLLHETNMGEQIIIGVIDTGVWPESEVFNDSGFGPVPSHWKGGCETGENFN 205

Query: 188 PAKACSRKLIGARYFVKGLEAAYGHPLNNSEFQDYWSPRDRSGHGTHVSSVASGFFVPNV 247
            +  C++KLIGA+YF+ G   A     N++   D+ SPRD  GHGTHVS++A G FVPN+
Sbjct: 206 SSN-CNKKLIGAKYFINGF-LAENESFNSTNSLDFISPRDLDGHGTHVSTIAGGSFVPNI 265

Query: 248 SYHGLAVGTVRGGAPHSRIAMYKVCWQVNGG---VCADIDILKAIDQAIYDGVDVLSLSL 307
           SY GLA GTVRGGAP + IAMYK CW ++      C+  DILKA+D+A++DGVDVLS+SL
Sbjct: 266 SYKGLAGGTVRGGAPRAHIAMYKACWYLDDDDTTTCSSADILKAMDEAMHDGVDVLSISL 325

Query: 308 GPSFPSYSDVDMRNGIAIGAFHAVAKGIVVVGAAGNSGPAAYSVSNIEPWLLTVAASSVD 367
           G S P Y + D+R+GI  GAFHAV KGI VV + GNSGP + +V+N  PW++TVAA+++D
Sbjct: 326 GSSVPLYGETDIRDGITTGAFHAVLKGITVVCSGGNSGPDSLTVTNTAPWIITVAATTLD 385

Query: 368 RSFLVAITLGNNWTTMGQGMFSGKLTKFHNLVYPEVSDLDDER---ACESLSLN-DTWAA 427
           RSF   +TLGNN   +GQ M++G    F +LVYPE     +E     CE L  N +    
Sbjct: 386 RSFATPLTLGNNKVILGQAMYTGPGLGFTSLVYPENPGNSNESFSGTCEELLFNSNRTME 445

Query: 428 GNVVLCFASDDYNDDTHNTSSSVKKVGGLGLIVAKNPTKAVEPFINNFPCVQISLDIGMQ 487
           G VVLCF +  Y     + +  VK+ GGLG+I+A++P  A++P +++FPCV +  ++G  
Sbjct: 446 GKVVLCFTTSPYGGAVLSAARYVKRAGGLGVIIARHPGYAIQPCLDDFPCVAVDWELGTD 505

Query: 488 ILNYIRSTRNPQVKIGASTTRVGQPLSTTVAYFSSRGPNSVAPAILKPDIAAPGVAILAA 547
           IL Y RS+ +P VKI  S T VGQP+ T VA FSSRGPNS+APAILKPDIAAPGV+ILAA
Sbjct: 506 ILLYTRSSGSPVVKIQPSKTLVGQPVGTKVATFSSRGPNSIAPAILKPDIAAPGVSILAA 565

Query: 548 VPPSDPKDRNSYAFISGTSMATPHVSAIVALLKTLHSHWSPAAIKSAIVTTAWSSDPYGA 607
              +   D+  +  +SGTSMA P +S + ALLK LH  WSPAAI+SAIVTTAW +DP+G 
Sbjct: 566 TTNTTFSDQ-GFIMLSGTSMAAPAISGVAALLKALHRDWSPAAIRSAIVTTAWKTDPFGE 625

Query: 608 VIFAEGQPMMSARPFDFGGGIVNPNKAVDPGLVYDMGMADYIQYFCAKGYNDSAISGITK 667
            IFAEG P   A PFD+GGG+VNP K+ +PGLVYDMG+ DY+ Y C+ GYN+++IS +  
Sbjct: 626 QIFAEGSPPKLADPFDYGGGLVNPEKSANPGLVYDMGLEDYVLYMCSVGYNETSISQLIG 685

Query: 668 RSISCPKRRPSILDINVPSITIPSLTHPVSLTRTVTNVGAINSSYKAAIEAPPGITIAIK 727
           ++  C   +PS+LD N+PSITIP+L   V++TRTVTNVG +NS Y+  +E P G  + + 
Sbjct: 686 KTTVCSNPKPSVLDFNLPSITIPNLKDEVTITRTVTNVGPLNSVYRVTVEPPLGFQVTVT 745

Query: 728 PRILKFNHKMKSISFTVTISSNHRVTTGYCFGSLTWLDGVHSVRIPVSVRTNI 774
           P  L FN   K + F V +S+ H+  TGY FGSLTW D +H+V IP+SVRT I
Sbjct: 746 PETLVFNSTTKKVYFKVKVSTTHKTNTGYYFGSLTWSDSLHNVTIPLSVRTQI 786

BLAST of Carg15037 vs. TAIR 10
Match: AT4G10540.1 (Subtilase family protein )

HSP 1 Score: 802.7 bits (2072), Expect = 2.5e-232
Identity = 413/773 (53.43%), Postives = 539/773 (69.73%), Query Frame = 0

Query: 8   KSSSTTIFLVCISVFTALDHLCTLHIRVAAKSNVYIVYMGQKPHDNEELLVKAHHGVLAS 67
           KS  T IF+  I     L+ L T      A+S V+IVY+G+K HD+ E + ++HH +L S
Sbjct: 2   KSCRTLIFVAII-----LNGLSTFVAHAGAESKVHIVYLGEKQHDDPEFVTESHHRMLWS 61

Query: 68  VLGSQEGSVDSLVYSYKYGFSGFAAKLTMAQAQMISELPSVVEVIPNRLHKMQTTRSWDY 127
           +LGS+E +  S+V+SY++GFSGFAAKLT +QA+ +++LP VV V P+  +++ TTR+WDY
Sbjct: 62  LLGSKEDAHSSMVHSYRHGFSGFAAKLTKSQAKKLADLPEVVHVTPDSFYQLDTTRTWDY 121

Query: 128 LQLSPRFPNSLLRKSRMGSGAIIGVLDTGIWPESEVFCDEGLGPVPSRWKGICESGELFS 187
           L LS   P +LL  + MG   IIG++D+G+WPESEVF D G+GPVPS WKG C SGE F+
Sbjct: 122 LGLSVANPKNLLNDTNMGEEVIIGIVDSGVWPESEVFNDNGIGPVPSHWKGGCVSGENFT 181

Query: 188 PAKACSRKLIGARYFVKGLEAAYGHPLNNSEFQDYWSPRDRSGHGTHVSSVASGFFVPNV 247
            ++ C++KLIGA+YF+ G  A +    N++E  D+ SPRDRSGHGTHV+++A G +VP++
Sbjct: 182 SSQ-CNKKLIGAKYFINGFLATH-ESFNSTESLDFISPRDRSGHGTHVATIAGGSYVPSI 241

Query: 248 SYHGLAVGTVRGGAPHSRIAMYKVCWQVNG---GVCADIDILKAIDQAIYDGVDVLSLSL 307
           SY GLA GTVRGGAP +RIAMYK CW ++      C+  DILKA+D+A++DGVDVLSLS+
Sbjct: 242 SYKGLAGGTVRGGAPRARIAMYKACWYLDRFDINTCSSADILKAMDEAMHDGVDVLSLSI 301

Query: 308 GPSFPSYSDVDMRNGIAIGAFHAVAKGIVVVGAAGNSGPAAYSVSNIEPWLLTVAASSVD 367
           G  FP + + D+R  IA GAFHAV KGI VV + GNSGPAA +V N  PW+LTVAA+++D
Sbjct: 302 GYRFPYFPETDVRAVIATGAFHAVLKGITVVCSGGNSGPAAQTVGNTAPWILTVAATTLD 361

Query: 368 RSFLVAITLGNNWTTMGQGMFSGKLTKFHNLVYPEVSDLDDER---ACESLSLNDTWA-A 427
           RSF   ITLGNN   +GQ M++G    F +LVYPE     +E     CE L  N     A
Sbjct: 362 RSFPTPITLGNNKLILGQAMYTGPELGFTSLVYPENPGNSNESFSGDCELLFFNSNHTMA 421

Query: 428 GNVVLCFASDDYNDDTHNTSSSVKKVGGLGLIVAKNPTKAVEPFINNFPCVQISLDIGMQ 487
           G VVLCF +        +  S VK+ GGLG+IVA+NP   + P  ++FPCV +  ++G  
Sbjct: 422 GKVVLCFTTSTRYITVSSAVSYVKEAGGLGVIVARNPGDNLSPCEDDFPCVAVDYELGTD 481

Query: 488 ILNYIRSTRNPQVKIGASTTRVGQPLSTTVAYFSSRGPNSVAPAILKPDIAAPGVAILAA 547
           IL YIRST  P VKI  S T VGQP+ T VA FSSRGPNS+ PAILKPDIAAPGV+ILAA
Sbjct: 482 ILLYIRSTGLPVVKIQPSKTLVGQPVGTKVADFSSRGPNSIEPAILKPDIAAPGVSILAA 541

Query: 548 VPPSDPKDRNSYAFISGTSMATPHVSAIVALLKTLHSHWSPAAIKSAIVTTAWSSDPYGA 607
              +   +   + F+SGTSMA P +S +VALLK LH  WSPAAI+SAIVTTAW +DP+G 
Sbjct: 542 TTTNKTFNDRGFIFLSGTSMAAPTISGVVALLKALHRDWSPAAIRSAIVTTAWRTDPFGE 601

Query: 608 VIFAEGQPMMSARPFDFGGGIVNPNKAVDPGLVYDMGMADYIQYFCAKGYNDSAISGITK 667
            IFAEG P   A PFD+GGG+VNP KA  PGLVYD+G+ DY+ Y C+ GYN+++IS +  
Sbjct: 602 QIFAEGSPRKLADPFDYGGGLVNPEKAAKPGLVYDLGLEDYVLYMCSVGYNETSISQLVG 661

Query: 668 RSISCPKRRPSILDINVPSITIPSLTHPVSLTRTVTNVGAINSSYKAAIEAPPGITIAIK 727
           +   C   +PS+LD N+PSITIP+L   V+LTRT+TNVG + S YK  IE P GI + + 
Sbjct: 662 KGTVCSNPKPSVLDFNLPSITIPNLKDEVTLTRTLTNVGQLESVYKVVIEPPIGIQVTVT 721

Query: 728 PRILKFNHKMKSISFTVTISSNHRVTTGYCFGSLTWLDGVHSVRIPVSVRTNI 774
           P  L FN   K +SF V +S+ H++ TGY FGSLTW D +H+V IP+SVRT I
Sbjct: 722 PETLLFNSTTKRVSFKVKVSTTHKINTGYFFGSLTWSDSLHNVTIPLSVRTQI 767

BLAST of Carg15037 vs. TAIR 10
Match: AT4G10550.1 (Subtilase family protein )

HSP 1 Score: 802.4 bits (2071), Expect = 3.3e-232
Identity = 406/769 (52.80%), Postives = 542/769 (70.48%), Query Frame = 0

Query: 12  TTIFLVCISVFTALDHLCTLHIRVAAKSNVYIVYMGQKPHDNEELLVKAHHGVLASVLGS 71
           T+I++V +S+   L+   +     +AK  V+IVY+G+K HD+ E + ++HH +L S+LGS
Sbjct: 6   TSIYVV-LSLVIFLNVQRSFVAESSAKRKVHIVYLGEKQHDDPEFVTESHHRMLWSLLGS 65

Query: 72  QEGSVDSLVYSYKYGFSGFAAKLTMAQAQMISELPSVVEVIPNRLHKMQTTRSWDYLQLS 131
           +E + DS+VYSY++GFSGFAAKLT +QA+ I++LP VV VIP+  +K+ TTR+WDYL LS
Sbjct: 66  KEDANDSMVYSYRHGFSGFAAKLTESQAKKIADLPDVVHVIPDSFYKLATTRTWDYLGLS 125

Query: 132 PRFPNSLLRKSRMGSGAIIGVLDTGIWPESEVFCDEGLGPVPSRWKGICESGELFSPAKA 191
              P SLL ++ MG   IIGV+DTG+WPESEVF D G GPVPS WKG CE+GE F+ +  
Sbjct: 126 AANPKSLLHETNMGEQIIIGVIDTGVWPESEVFNDSGFGPVPSHWKGGCETGENFNSSN- 185

Query: 192 CSRKLIGARYFVKGLEAAYGHPLNNSEFQDYWSPRDRSGHGTHVSSVASGFFVPNVSYHG 251
           C++KLIGA+YF+ G   A     N++   D+ SPRD  GHGTHVS++A G FVPN+SY G
Sbjct: 186 CNKKLIGAKYFINGF-LAENESFNSTNSLDFISPRDLDGHGTHVSTIAGGSFVPNISYKG 245

Query: 252 LAVGTVRGGAPHSRIAMYKVCWQVNGG---VCADIDILKAIDQAIYDGVDVLSLSLGPSF 311
           LA GTVRGGAP + IAMYK CW ++      C+  DILKA+D+A++DGVDVLS+SLG S 
Sbjct: 246 LAGGTVRGGAPRAHIAMYKACWYLDDDDTTTCSSADILKAMDEAMHDGVDVLSISLGSSV 305

Query: 312 PSYSDVDMRNGIAIGAFHAVAKGIVVVGAAGNSGPAAYSVSNIEPWLLTVAASSVDRSFL 371
           P Y + D+R+GI  GAFHAV KGI VV + GNSGP + +V+N  PW++TVAA+++DRSF 
Sbjct: 306 PLYGETDIRDGITTGAFHAVLKGITVVCSGGNSGPDSLTVTNTAPWIITVAATTLDRSFA 365

Query: 372 VAITLGNNWTTMGQGMFSGKLTKFHNLVYPEVSDLDDER---ACESLSLN-DTWAAGNVV 431
             +TLGNN   +GQ M++G    F +LVYPE     +E     CE L  N +    G VV
Sbjct: 366 TPLTLGNNKVILGQAMYTGPGLGFTSLVYPENPGNSNESFSGTCEELLFNSNRTMEGKVV 425

Query: 432 LCFASDDYNDDTHNTSSSVKKVGGLGLIVAKNPTKAVEPFINNFPCVQISLDIGMQILNY 491
           LCF +  Y     + +  VK+ GGLG+I+A++P  A++P +++FPCV +  ++G  IL Y
Sbjct: 426 LCFTTSPYGGAVLSAARYVKRAGGLGVIIARHPGYAIQPCLDDFPCVAVDWELGTDILLY 485

Query: 492 IRSTRNPQVKIGASTTRVGQPLSTTVAYFSSRGPNSVAPAILKPDIAAPGVAILAAVPPS 551
            RS+ +P VKI  S T VGQP+ T VA FSSRGPNS+APAILKPDIAAPGV+ILAA   +
Sbjct: 486 TRSSGSPVVKIQPSKTLVGQPVGTKVATFSSRGPNSIAPAILKPDIAAPGVSILAATTNT 545

Query: 552 DPKDRNSYAFISGTSMATPHVSAIVALLKTLHSHWSPAAIKSAIVTTAWSSDPYGAVIFA 611
              D+  +  +SGTSMA P +S + ALLK LH  WSPAAI+SAIVTTAW +DP+G  IFA
Sbjct: 546 TFSDQ-GFIMLSGTSMAAPAISGVAALLKALHRDWSPAAIRSAIVTTAWKTDPFGEQIFA 605

Query: 612 EGQPMMSARPFDFGGGIVNPNKAVDPGLVYDMGMADYIQYFCAKGYNDSAISGITKRSIS 671
           EG P   A PFD+GGG+VNP K+ +PGLVYDMG+ DY+ Y C+ GYN+++IS +  ++  
Sbjct: 606 EGSPPKLADPFDYGGGLVNPEKSANPGLVYDMGLEDYVLYMCSVGYNETSISQLIGKTTV 665

Query: 672 CPKRRPSILDINVPSITIPSLTHPVSLTRTVTNVGAINSSYKAAIEAPPGITIAIKPRIL 731
           C   +PS+LD N+PSITIP+L   V++TRTVTNVG +NS Y+  +E P G  + + P  L
Sbjct: 666 CSNPKPSVLDFNLPSITIPNLKDEVTITRTVTNVGPLNSVYRVTVEPPLGFQVTVTPETL 725

Query: 732 KFNHKMKSISFTVTISSNHRVTTGYCFGSLTWLDGVHSVRIPVSVRTNI 774
            FN   K + F V +S+ H+  TGY FGSLTW D +H+V IP+SVRT I
Sbjct: 726 VFNSTTKKVYFKVKVSTTHKTNTGYYFGSLTWSDSLHNVTIPLSVRTQI 770