Carg15024 (gene) Silver-seed gourd (SMH-JMG-627) v2

Overview


Name	Carg15024
Type	gene
Organism	Cucurbita argyrosperma subsp. argyrosperma cv. SMH-JMG-627 (Silver-seed gourd (SMH-JMG-627) v2)
Description	Kazal-type serine protease inhibitor
Location	Carg_Chr16: 160520 .. 160912 (+)
RNA-Seq Expression	Carg15024
Synteny	Carg15024

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGGTCCCCTTCTCCAAACCCCCCATTTCCATCCTTCTCTTCTTTTCCCTTCTTATCTTCGTTTTTGAAGTCCGATCGCAGCATGTTTCCTCCGCCATCCGCTTGCCTTCGGAAGCCACCAATAACGACCGCGATCTAGATCTCTGCCCTGTGTCCCTGCCGTCTTCTTGCCCTGTGAAATGCTTTAGGACGGACCCAGTCTGCGGCGTTGATGGCCTTACCTACTGGTGTGGTTGCGCCGATGCTCTGTGTTCAGGTGTCAAGGTCGCCAAGATGGGATTTTGTGAGGTCGGCAACGGCGGTTCTGCTCCTATTCCTGGTCAGGCCCTCCTCCTGGTGCATATTTTGTGGTTGATCATCCTTGGAGTTTCCGTCTTGTTTGGACTCTTCTGA

mRNA sequence

ATGGTCCCCTTCTCCAAACCCCCCATTTCCATCCTTCTCTTCTTTTCCCTTCTTATCTTCGTTTTTGAAGTCCGATCGCAGCATGTTTCCTCCGCCATCCGCTTGCCTTCGGAAGCCACCAATAACGACCGCGATCTAGATCTCTGCCCTGTGTCCCTGCCGTCTTCTTGCCCTGTGAAATGCTTTAGGACGGACCCAGTCTGCGGCGTTGATGGCCTTACCTACTGGTGTGGTTGCGCCGATGCTCTGTGTTCAGGTGTCAAGGTCGCCAAGATGGGATTTTGTGAGGTCGGCAACGGCGGTTCTGCTCCTATTCCTGGTCAGGCCCTCCTCCTGGTGCATATTTTGTGGTTGATCATCCTTGGAGTTTCCGTCTTGTTTGGACTCTTCTGA

Coding sequence (CDS)

ATGGTCCCCTTCTCCAAACCCCCCATTTCCATCCTTCTCTTCTTTTCCCTTCTTATCTTCGTTTTTGAAGTCCGATCGCAGCATGTTTCCTCCGCCATCCGCTTGCCTTCGGAAGCCACCAATAACGACCGCGATCTAGATCTCTGCCCTGTGTCCCTGCCGTCTTCTTGCCCTGTGAAATGCTTTAGGACGGACCCAGTCTGCGGCGTTGATGGCCTTACCTACTGGTGTGGTTGCGCCGATGCTCTGTGTTCAGGTGTCAAGGTCGCCAAGATGGGATTTTGTGAGGTCGGCAACGGCGGTTCTGCTCCTATTCCTGGTCAGGCCCTCCTCCTGGTGCATATTTTGTGGTTGATCATCCTTGGAGTTTCCGTCTTGTTTGGACTCTTCTGA

Protein sequence

MVPFSKPPISILLFFSLLIFVFEVRSQHVSSAIRLPSEATNNDRDLDLCPVSLPSSCPVKCFRTDPVCGVDGLTYWCGCADALCSGVKVAKMGFCEVGNGGSAPIPGQALLLVHILWLIILGVSVLFGLF

Homology

[Close]

BLAST of Carg15024 vs. NCBI nr
Match: XP_022922853.1 (uncharacterized protein LOC111430710 [Cucurbita moschata] >XP_022985062.1 uncharacterized protein LOC111483145 [Cucurbita maxima] >XP_023552497.1 uncharacterized protein LOC111810142 [Cucurbita pepo subsp. pepo] >KAG7014664.1 hypothetical protein SDJN02_22293, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 263.8 bits (673), Expect = 7.6e-67
Identity = 130/130 (100.00%), Postives = 130/130 (100.00%), Query Frame = 0


Query: 1   MVPFSKPPISILLFFSLLIFVFEVRSQHVSSAIRLPSEATNNDRDLDLCPVSLPSSCPVK 60
           MVPFSKPPISILLFFSLLIFVFEVRSQHVSSAIRLPSEATNNDRDLDLCPVSLPSSCPVK
Sbjct: 1   MVPFSKPPISILLFFSLLIFVFEVRSQHVSSAIRLPSEATNNDRDLDLCPVSLPSSCPVK 60

Query: 61  CFRTDPVCGVDGLTYWCGCADALCSGVKVAKMGFCEVGNGGSAPIPGQALLLVHILWLII 120
           CFRTDPVCGVDGLTYWCGCADALCSGVKVAKMGFCEVGNGGSAPIPGQALLLVHILWLII
Sbjct: 61  CFRTDPVCGVDGLTYWCGCADALCSGVKVAKMGFCEVGNGGSAPIPGQALLLVHILWLII 120

Query: 121 LGVSVLFGLF 131
           LGVSVLFGLF
Sbjct: 121 LGVSVLFGLF 130

[Close]

BLAST of Carg15024 vs. NCBI nr
Match: XP_038892451.1 (uncharacterized protein LOC120081544 [Benincasa hispida])

HSP 1 Score: 242.3 bits (617), Expect = 2.4e-60
Identity = 119/130 (91.54%), Postives = 124/130 (95.38%), Query Frame = 0


Query: 1   MVPFSKPPISILLFFSLLIFVFEVRSQHVSSAIRLPSEATNNDRDLDLCPVSLPSSCPVK 60
           M  FS+PPISI+ FFSLLIFVF VRS+ VSSAIRLPSEATNND D+DLCPVS+PSSCPVK
Sbjct: 1   MFLFSRPPISIVFFFSLLIFVFAVRSEDVSSAIRLPSEATNNDGDVDLCPVSVPSSCPVK 60

Query: 61  CFRTDPVCGVDGLTYWCGCADALCSGVKVAKMGFCEVGNGGSAPIPGQALLLVHILWLII 120
           CFRTDPVCGVDGLTYWCGCADALCSGVKVAKMGFCEVGNGGSAPIPGQALLLVHILWLII
Sbjct: 61  CFRTDPVCGVDGLTYWCGCADALCSGVKVAKMGFCEVGNGGSAPIPGQALLLVHILWLII 120

Query: 121 LGVSVLFGLF 131
           LGVSVLFGLF
Sbjct: 121 LGVSVLFGLF 130

[Close]

BLAST of Carg15024 vs. NCBI nr
Match: XP_016902950.1 (PREDICTED: uncharacterized protein LOC107991954 [Cucumis melo] >KAA0056649.1 uncharacterized protein E6C27_scaffold288G001800 [Cucumis melo var. makuwa] >TYK04610.1 uncharacterized protein E5676_scaffold351G00140 [Cucumis melo var. makuwa])

HSP 1 Score: 237.3 bits (604), Expect = 7.6e-59
Identity = 115/130 (88.46%), Postives = 122/130 (93.85%), Query Frame = 0


Query: 1   MVPFSKPPISILLFFSLLIFVFEVRSQHVSSAIRLPSEATNNDRDLDLCPVSLPSSCPVK 60
           M+ FS PP+SI+ FFSLLIFVF VRS+ VSSAIRLPSEATNND D+DLCPVS+PS CPVK
Sbjct: 1   MILFSAPPVSIVFFFSLLIFVFAVRSEDVSSAIRLPSEATNNDGDVDLCPVSVPSYCPVK 60

Query: 61  CFRTDPVCGVDGLTYWCGCADALCSGVKVAKMGFCEVGNGGSAPIPGQALLLVHILWLII 120
           CFRTDPVCGVDG+TYWCGCADALCSGVKVAKMGFCEVGNGGSAPIPGQAL LVHILWLII
Sbjct: 61  CFRTDPVCGVDGVTYWCGCADALCSGVKVAKMGFCEVGNGGSAPIPGQALFLVHILWLII 120

Query: 121 LGVSVLFGLF 131
           LGVSVLFGLF
Sbjct: 121 LGVSVLFGLF 130

[Close]

BLAST of Carg15024 vs. NCBI nr
Match: XP_004140750.1 (uncharacterized protein LOC101210178 [Cucumis sativus])

HSP 1 Score: 234.2 bits (596), Expect = 6.4e-58
Identity = 115/130 (88.46%), Postives = 121/130 (93.08%), Query Frame = 0


Query: 1   MVPFSKPPISILLFFSLLIFVFEVRSQHVSSAIRLPSEATNNDRDLDLCPVSLPSSCPVK 60
           M  FS+P ISI+  F LLIFVF VRS+ VSSAIRLPSEATNND D+DLCPVS+PSSCPVK
Sbjct: 1   MTLFSRPQISIIFLFLLLIFVFPVRSEDVSSAIRLPSEATNNDGDVDLCPVSVPSSCPVK 60

Query: 61  CFRTDPVCGVDGLTYWCGCADALCSGVKVAKMGFCEVGNGGSAPIPGQALLLVHILWLII 120
           CFRTDPVCGVDG+TYWCGCADALCSGVKVAKMGFCEVGNGGSAPIPGQALLLVHILWLII
Sbjct: 61  CFRTDPVCGVDGVTYWCGCADALCSGVKVAKMGFCEVGNGGSAPIPGQALLLVHILWLII 120

Query: 121 LGVSVLFGLF 131
           LGVSVLFGLF
Sbjct: 121 LGVSVLFGLF 130

[Close]

BLAST of Carg15024 vs. NCBI nr
Match: TYK23009.1 (Serine-type endopeptidase inhibitor [Cucumis melo var. makuwa])

HSP 1 Score: 233.0 bits (593), Expect = 1.4e-57
Identity = 115/130 (88.46%), Postives = 120/130 (92.31%), Query Frame = 0


Query: 1   MVPFSKPPISILLFFSLLIFVFEVRSQHVSSAIRLPSEATNNDRDLDLCPVSLPSSCPVK 60
           M  FS PPISI+  F LLIFVF VRS+ VSSAIRLPSEATNND D+DLCPVS+PSSCPVK
Sbjct: 1   MTLFSTPPISIIFLFLLLIFVFPVRSEDVSSAIRLPSEATNNDGDVDLCPVSVPSSCPVK 60

Query: 61  CFRTDPVCGVDGLTYWCGCADALCSGVKVAKMGFCEVGNGGSAPIPGQALLLVHILWLII 120
           CFRTDPVCGVDG+TYWCGCADALCSGVKVAKMGFCEVGNGGSA IPGQALLLVHILWLII
Sbjct: 61  CFRTDPVCGVDGVTYWCGCADALCSGVKVAKMGFCEVGNGGSASIPGQALLLVHILWLII 120

Query: 121 LGVSVLFGLF 131
           LGVSVLFGLF
Sbjct: 121 LGVSVLFGLF 130

[Close]

BLAST of Carg15024 vs. ExPASy TrEMBL
Match: A0A6J1J3U9 (uncharacterized protein LOC111483145 OS=Cucurbita maxima OX=3661 GN=LOC111483145 PE=4 SV=1)

HSP 1 Score: 263.8 bits (673), Expect = 3.7e-67
Identity = 130/130 (100.00%), Postives = 130/130 (100.00%), Query Frame = 0


Query: 1   MVPFSKPPISILLFFSLLIFVFEVRSQHVSSAIRLPSEATNNDRDLDLCPVSLPSSCPVK 60
           MVPFSKPPISILLFFSLLIFVFEVRSQHVSSAIRLPSEATNNDRDLDLCPVSLPSSCPVK
Sbjct: 1   MVPFSKPPISILLFFSLLIFVFEVRSQHVSSAIRLPSEATNNDRDLDLCPVSLPSSCPVK 60

Query: 61  CFRTDPVCGVDGLTYWCGCADALCSGVKVAKMGFCEVGNGGSAPIPGQALLLVHILWLII 120
           CFRTDPVCGVDGLTYWCGCADALCSGVKVAKMGFCEVGNGGSAPIPGQALLLVHILWLII
Sbjct: 61  CFRTDPVCGVDGLTYWCGCADALCSGVKVAKMGFCEVGNGGSAPIPGQALLLVHILWLII 120

Query: 121 LGVSVLFGLF 131
           LGVSVLFGLF
Sbjct: 121 LGVSVLFGLF 130

[Close]

BLAST of Carg15024 vs. ExPASy TrEMBL
Match: A0A6J1E7Y6 (uncharacterized protein LOC111430710 OS=Cucurbita moschata OX=3662 GN=LOC111430710 PE=4 SV=1)

HSP 1 Score: 263.8 bits (673), Expect = 3.7e-67
Identity = 130/130 (100.00%), Postives = 130/130 (100.00%), Query Frame = 0


Query: 1   MVPFSKPPISILLFFSLLIFVFEVRSQHVSSAIRLPSEATNNDRDLDLCPVSLPSSCPVK 60
           MVPFSKPPISILLFFSLLIFVFEVRSQHVSSAIRLPSEATNNDRDLDLCPVSLPSSCPVK
Sbjct: 1   MVPFSKPPISILLFFSLLIFVFEVRSQHVSSAIRLPSEATNNDRDLDLCPVSLPSSCPVK 60

Query: 61  CFRTDPVCGVDGLTYWCGCADALCSGVKVAKMGFCEVGNGGSAPIPGQALLLVHILWLII 120
           CFRTDPVCGVDGLTYWCGCADALCSGVKVAKMGFCEVGNGGSAPIPGQALLLVHILWLII
Sbjct: 61  CFRTDPVCGVDGLTYWCGCADALCSGVKVAKMGFCEVGNGGSAPIPGQALLLVHILWLII 120

Query: 121 LGVSVLFGLF 131
           LGVSVLFGLF
Sbjct: 121 LGVSVLFGLF 130

[Close]

BLAST of Carg15024 vs. ExPASy TrEMBL
Match: A0A5A7UT13 (Uncharacterized protein OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold351G00140 PE=4 SV=1)

HSP 1 Score: 237.3 bits (604), Expect = 3.7e-59
Identity = 115/130 (88.46%), Postives = 122/130 (93.85%), Query Frame = 0


Query: 1   MVPFSKPPISILLFFSLLIFVFEVRSQHVSSAIRLPSEATNNDRDLDLCPVSLPSSCPVK 60
           M+ FS PP+SI+ FFSLLIFVF VRS+ VSSAIRLPSEATNND D+DLCPVS+PS CPVK
Sbjct: 1   MILFSAPPVSIVFFFSLLIFVFAVRSEDVSSAIRLPSEATNNDGDVDLCPVSVPSYCPVK 60

Query: 61  CFRTDPVCGVDGLTYWCGCADALCSGVKVAKMGFCEVGNGGSAPIPGQALLLVHILWLII 120
           CFRTDPVCGVDG+TYWCGCADALCSGVKVAKMGFCEVGNGGSAPIPGQAL LVHILWLII
Sbjct: 61  CFRTDPVCGVDGVTYWCGCADALCSGVKVAKMGFCEVGNGGSAPIPGQALFLVHILWLII 120

Query: 121 LGVSVLFGLF 131
           LGVSVLFGLF
Sbjct: 121 LGVSVLFGLF 130

[Close]

BLAST of Carg15024 vs. ExPASy TrEMBL
Match: A0A1S4E4Q0 (uncharacterized protein LOC107991954 OS=Cucumis melo OX=3656 GN=LOC107991954 PE=4 SV=1)

HSP 1 Score: 237.3 bits (604), Expect = 3.7e-59
Identity = 115/130 (88.46%), Postives = 122/130 (93.85%), Query Frame = 0


Query: 1   MVPFSKPPISILLFFSLLIFVFEVRSQHVSSAIRLPSEATNNDRDLDLCPVSLPSSCPVK 60
           M+ FS PP+SI+ FFSLLIFVF VRS+ VSSAIRLPSEATNND D+DLCPVS+PS CPVK
Sbjct: 1   MILFSAPPVSIVFFFSLLIFVFAVRSEDVSSAIRLPSEATNNDGDVDLCPVSVPSYCPVK 60

Query: 61  CFRTDPVCGVDGLTYWCGCADALCSGVKVAKMGFCEVGNGGSAPIPGQALLLVHILWLII 120
           CFRTDPVCGVDG+TYWCGCADALCSGVKVAKMGFCEVGNGGSAPIPGQAL LVHILWLII
Sbjct: 61  CFRTDPVCGVDGVTYWCGCADALCSGVKVAKMGFCEVGNGGSAPIPGQALFLVHILWLII 120

Query: 121 LGVSVLFGLF 131
           LGVSVLFGLF
Sbjct: 121 LGVSVLFGLF 130

[Close]

BLAST of Carg15024 vs. ExPASy TrEMBL
Match: A0A0A0L616 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_3G184020 PE=4 SV=1)

HSP 1 Score: 234.2 bits (596), Expect = 3.1e-58
Identity = 115/130 (88.46%), Postives = 121/130 (93.08%), Query Frame = 0


Query: 1   MVPFSKPPISILLFFSLLIFVFEVRSQHVSSAIRLPSEATNNDRDLDLCPVSLPSSCPVK 60
           M  FS+P ISI+  F LLIFVF VRS+ VSSAIRLPSEATNND D+DLCPVS+PSSCPVK
Sbjct: 1   MTLFSRPQISIIFLFLLLIFVFPVRSEDVSSAIRLPSEATNNDGDVDLCPVSVPSSCPVK 60

Query: 61  CFRTDPVCGVDGLTYWCGCADALCSGVKVAKMGFCEVGNGGSAPIPGQALLLVHILWLII 120
           CFRTDPVCGVDG+TYWCGCADALCSGVKVAKMGFCEVGNGGSAPIPGQALLLVHILWLII
Sbjct: 61  CFRTDPVCGVDGVTYWCGCADALCSGVKVAKMGFCEVGNGGSAPIPGQALLLVHILWLII 120

Query: 121 LGVSVLFGLF 131
           LGVSVLFGLF
Sbjct: 121 LGVSVLFGLF 130

[Close]

BLAST of Carg15024 vs. TAIR 10
Match: AT4G01575.1 (serine protease inhibitor, Kazal-type family protein )

HSP 1 Score: 137.9 bits (346), Expect = 5.8e-33
Identity = 67/130 (51.54%), Postives = 91/130 (70.00%), Query Frame = 0


Query: 7   PPISILLFFSLLIF----VFEVRSQHVSSAIRLPSEATNNDRDL-DLCP-VSLPSSCPVK 66
           P ++++ F  L++     VF   S      IRLPSE  N +++  + C  ++ P+SCPV+
Sbjct: 15  PSLAVIAFLFLILLNLSSVFADPSTEGGEIIRLPSEKINGEKNRGEFCEGIAKPASCPVQ 74

Query: 67  CFRTDPVCGVDGLTYWCGCADALCSGVKVAKMGFCEVGNGGSAPIPGQALLLVHILWLII 126
           CFR DPVCG D +TYWCGCADALC GV+V K G C+VGNG    +PGQALLL+HI+W+++
Sbjct: 75  CFRPDPVCGEDSVTYWCGCADALCHGVRVVKQGACDVGNGVGLSVPGQALLLIHIVWMML 134

Query: 127 LGVSVLFGLF 131
           LG S+LFGLF
Sbjct: 135 LGFSILFGLF 144

[Close]

BLAST of Carg15024 vs. TAIR 10
Match: AT3G61980.1 (serine protease inhibitor, Kazal-type family protein )

HSP 1 Score: 115.9 bits (289), Expect = 2.4e-26
Identity = 51/85 (60.00%), Postives = 62/85 (72.94%), Query Frame = 0


Query: 47  DLCP-VSLPSSCPVKCFRTDPVCGVDGLTYWCGCADALCSGVKVAKMGFCEVGNGGSAPI 106
           D+CP V     C + CFR DPVCG DG+TYWCGC DA C G +V K G C+ GN GSA +
Sbjct: 33  DVCPRVKDRGGCTINCFRADPVCGTDGVTYWCGCPDAACHGARVVKKGACDTGNAGSASV 92

Query: 107 PGQALLLVHILWLIILGVSVLFGLF 131
           PGQALLL+HI+WL +LG+S+L G F
Sbjct: 93  PGQALLLIHIVWLFLLGLSLLVGGF 117

The following BLAST results are available for this feature:

BLAST of Carg15024 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_022922853.1	7.6e-67	100.00	uncharacterized protein LOC111430710 [Cucurbita moschata] >XP_022985062.1 unchar...	[more]
XP_038892451.1	2.4e-60	91.54	uncharacterized protein LOC120081544 [Benincasa hispida]	[more]
XP_016902950.1	7.6e-59	88.46	PREDICTED: uncharacterized protein LOC107991954 [Cucumis melo] >KAA0056649.1 unc...	[more]
XP_004140750.1	6.4e-58	88.46	uncharacterized protein LOC101210178 [Cucumis sativus]	[more]
TYK23009.1	1.4e-57	88.46	Serine-type endopeptidase inhibitor [Cucumis melo var. makuwa]	[more]

BLAST of Carg15024 vs. NCBI nr


Match Name	E-value	Identity	Description

BLAST of Carg15024 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1J3U9	3.7e-67	100.00	uncharacterized protein LOC111483145 OS=Cucurbita maxima OX=3661 GN=LOC111483145...	[more]
A0A6J1E7Y6	3.7e-67	100.00	uncharacterized protein LOC111430710 OS=Cucurbita moschata OX=3662 GN=LOC1114307...	[more]
A0A5A7UT13	3.7e-59	88.46	Uncharacterized protein OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold...	[more]
A0A1S4E4Q0	3.7e-59	88.46	uncharacterized protein LOC107991954 OS=Cucumis melo OX=3656 GN=LOC107991954 PE=...	[more]
A0A0A0L616	3.1e-58	88.46	Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_3G184020 PE=4 SV=1	[more]

BLAST of Carg15024 vs. TAIR 10


Match Name	E-value	Identity	Description
AT4G01575.1	5.8e-33	51.54	serine protease inhibitor, Kazal-type family protein	[more]
AT3G61980.1	2.4e-26	60.00	serine protease inhibitor, Kazal-type family protein	[more]

InterPro

Analysis Name: InterPro Annotations of Silver-seed gourd (SMH-JMG-627) v2
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
None	No IPR available	PANTHER	PTHR34376	SERINE PROTEASE INHIBITOR, KAZAL-TYPE FAMILY PROTEIN	coord: 4..130

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Carg15024-RA	Carg15024-RA	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
cellular_component	GO:0016021	integral component of membrane