Carg14875 (gene) Silver-seed gourd (SMH-JMG-627) v2

Overview
NameCarg14875
Typegene
OrganismCucurbita argyrosperma subsp. argyrosperma cv. SMH-JMG-627 (Silver-seed gourd (SMH-JMG-627) v2)
Descriptionprotein NRT1/ PTR FAMILY 7.3-like
LocationCarg_Chr14: 13762911 .. 13766507 (-)
RNA-Seq ExpressionCarg14875
SyntenyCarg14875
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRpolypeptideCDSthree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
CACTTGCTTGCTCTTCAATGGCTTGTCTCGATATCTCTTGTTTGCGTCAGGTTAGTAACCTAAATTTTTGTTCACGTGTTCGAGCCAGTTTACGTGCACGTACGAATAAGATCTAGAGCTTTCGCTAGCTTTTTCGAGTACTCACGGATGAACATCGGTTACTTTGAATTGTAAACGTGACAATTTGACTACATTTGGTTGTCGAGATAACGAGTAAAAGTTTTATACGTCGAGTTTTAGGGATCAGAAATAAAGTGGAAAACCACTCGTGTTCTTAAAAACTTTACTAATGTTCTTCTAAATTCTTCTTTTGAACTTTTTTTTTTTTTGTTCAGATTTGTAATTTTGAGATTCTTGATTCTGATTTCCATTTTTTAATTTATTTTTTTTATGTTTTTTTAGAGTACTATGCAGAGGAAGAAGACGACGATGACGACGACAGCTGAGAAAGAGGTGCTTACGCTTGACGGAGCTGTTGACCGACATGGTCGTCCCGCTGTTCGGGGCAAAACTGGAACTTGGGTTGCTGGGATCTTGCTACTTGGTACGTTCGTCTTGGAACATACCATGCATTTTGACGTGTTTTTAATGACCCAGAAAGTATTCGTGAGATTTACGTAGATGAGTAACTTGAGCTAATAAGTTTAAATAATTTGTGTATTAATGGCAGTGAACCAAGGGTTGGCTACGTTGGCGTTTTTTGGAGTTGGAGTGAACTTGGTGCTGTTTCTAACGAGGGTTATAGGACAAGACAATGCGGCAGCGGCCAATAACGTTAGCAAGTGGACAGGAACTGTCTACATTTTCTCTCTTCTTGGAGCCTTCCTTAGTGATTCGTACTTAGGAAGGTTCAAGACGTGTGCCATATTTCAAGCTATTTTCGTCTTGGTAAGCCGTTTTTGTTTTATTCTTTTCGAACTAATTACTTTTGTTGAGTGAACTGTTTTCGATTGTTCAATCACTTTTGTCATATGAAACTTGCCGTCACGTTTGTGAATTTTCAATCATTCATCTTTATTGTCATGTTTTTATATGTCAATAATGAAACAATATGACATAACGTAAAGATTTAGCGTGCAGTGTGGATGAAATTATTGAGTTTTTGTAGCTATTTGCTAAAACTATTCGAATCAAAATGGTAGGGCTTTTATGTTAGAAATTGGAATTTAATAATTTTAATAAAATGATAATCTGAATAAAAAATAAATATAATTCTAATTTGTAAAATTTGTAGTGGCTACTTTATGAACGACAGACGGCGTCGTTTAGTGAGAAAAAGTAAAAAAAGGAAAAAAGAAAAAGAAAAAGTTGCCTTTTTCTTTGACATAAACCTAAACAAATATGTTTTTTTTGATGTAAACCACAAAACAAATGTTCAATAAAGTGGAAGCTGAAGGTAAATGTATGTTAGAAAGTGACCCAATAATGTAAATCAATAAATAATAATTATAATATTTGGCCCCCACATATGACTTTTTTAAACTTCAAATTATTTGGGTCGTTTTTTTTTTGTTGGGTTCGATCATGATACTTATGGGTACGAGGATCTGAACTAATATGTCTTGGTTGATGGCTATATCTTAACGTACGAGGATCTTAAGGCTAATGAGCTATATCTTAATTTGAACGTTAACCCTTTAGCTGATAAGCTATATCTTAACGAACGAGAATCTAGGCGTTGACGTAAGTTTTGTTTACCTTTTTATTATTCTTGTTGGGCAGGGTCTAGCTTCACTATCAATAACATCCGGCCTATTTCTGTTGAAACCCAAAGGGTGTGGAGATGAGCATACTCCATGCGATTCTCACTCCTCCTACCACATTGCCCTGTTCTATCTCTCAGTATACTTGGTAGCCCTTGGCAATGGAGGATACCAACCCAACATAGCCACATTTGGTGCAGACCAGTTTGATGAAGAAGACCCTAAGGAAGGCCTCTCCAAAATTGCCTTCTTCAGCTACTTCTACTTGGCTCTCAACCTTGGCTCACTGTTCTCCAACACTATTTTGGGTTACTTTGAAGATGAAGGAATGTGGGTGATTGGTTTCTGGGCTTCTACTGCCTCTGCTGCTATGGCTTTGATCTTGTTCCTCTGTGGAATCCCTAGGTATAGACATTTTACTCCTAAAGGAAATCCCCTCTCTAGGGTTTCTCAAGTGCTGGTTGCAGCTACTAGGAAATGGAAGGTTCAGATTATGCCTAATTCTGAGGGATTGTTTGATGAGGATGAGAAGGATTTGGTTAGTAACAATGGAGCTAGGAAAATTCTACACACCAATGGATTCAGGTAAGTTTCATCATCAATTTCTACTTGGATTGAATGTTTGATTTCCAGAAACATGAATGTAATAGTGTGGATGTGTGATTGTGTCGTTGTTTTACAGATTTTTGGACAGAGCAGCAGCAATTACATCACCTGAGATGGATCAATTTGAAGATGGAACTCGGAATCCATGGCGTCTCTGTACGGTTACTCAAGTCGAAGAAGTCAAATGCGTTCTCAGACTCCTCCCGATTTGGCTTTGCACGATCCTCTACTCCGTCGTCTTCACTCAAATGGCTTCTCTGTTCGTCGAGCAAGGCGCCGTCATGAGAACCAACATTTCTACATTCCACATCCCTCCCGCTAGCATGTCGAGCTTCGACATTCTCAGCGTCGCAGCGTTCATCTTCATCTACCGTAGAGTAATCGATCCAATCTACACTCGATTAACCAAATCGAGCCTCACGGAGCTTCAAAGAATGGGGATCGGACTTGTAATCGCAATCTGTGCCATGGTATCCGCCGGAACCGTCGAGGTTTTTCGATTAAGATACGCCGACAAAGATTGCCTCCATTGTGAGGAATCGAGTTCCCTAAGCATTTTCTGGCAGATTCCTCAGTACGTACTGATCGGAGCATCTGAGGTTTTCATGTACGTCGGCCAACTGGAATTCTTCAACAGTCAGGCACCTGACGGACTCAAAAGCTTCGGTAGTGCTCTCTGTATGACCTCGATTTCCTTCGGCAACTACGTTAGCAGCTTGCTGGTGACGATCGTGATGAAGGTCTCAACGAACGGTAACATGCCGGGATGGATCCCTGGAAACCTAAACAGAGGCCATTTAGACCGATTTTTCTTCCTCTTAGCCATCCTCACGGCGGCGGATCTCGTCGTCTACATCATCTGCGCCAAATGGTACAAATACATCAAATTCGAGAGCCGTGAGGCCGCCGCCGCCACCGCCACCGCCGCTGTCTAGGGCTTCGCTCCGACCGAAAATGGCGGCAGAATAAAGTTGGAGGTAGAAGAATGTCAATTCCAACGCCGTTTGATCCAATTTCGATGGTTCATAATCGAATTATTTGATTAGAAGAAAAATAAATAAATAAGTAGTTTTGAGGTGGTTCGATCGATGACCGTTGAAATTGGACCGGACATAATCGTGGCTGTAGATTTGAAGACGAAACCCACTCCGGTCGGAGGATAACCGGGCGGTTGACGCTATGAGACTTGAGAGATGATTCCGATTACTTCTCCAGTTGAGCGATGTAATATTTAATTCAACGTTTCATTGTTGTGTTATAATGAAATAAAAAATGTCATTTTTCTCTA

mRNA sequence

CACTTGCTTGCTCTTCAATGGCTTGTCTCGATATCTCTTGTTTGCGTCAGAGTACTATGCAGAGGAAGAAGACGACGATGACGACGACAGCTGAGAAAGAGGTGCTTACGCTTGACGGAGCTGTTGACCGACATGGTCGTCCCGCTGTTCGGGGCAAAACTGGAACTTGGGTTGCTGGGATCTTGCTACTTGTGAACCAAGGGTTGGCTACGTTGGCGTTTTTTGGAGTTGGAGTGAACTTGGTGCTGTTTCTAACGAGGGTTATAGGACAAGACAATGCGGCAGCGGCCAATAACGTTAGCAAGTGGACAGGAACTGTCTACATTTTCTCTCTTCTTGGAGCCTTCCTTAGTGATTCGTACTTAGGAAGGTTCAAGACGTGTGCCATATTTCAAGCTATTTTCGTCTTGGGTCTAGCTTCACTATCAATAACATCCGGCCTATTTCTGTTGAAACCCAAAGGGTGTGGAGATGAGCATACTCCATGCGATTCTCACTCCTCCTACCACATTGCCCTGTTCTATCTCTCAGTATACTTGGTAGCCCTTGGCAATGGAGGATACCAACCCAACATAGCCACATTTGGTGCAGACCAGTTTGATGAAGAAGACCCTAAGGAAGGCCTCTCCAAAATTGCCTTCTTCAGCTACTTCTACTTGGCTCTCAACCTTGGCTCACTGTTCTCCAACACTATTTTGGGTTACTTTGAAGATGAAGGAATGTGGGTGATTGGTTTCTGGGCTTCTACTGCCTCTGCTGCTATGGCTTTGATCTTGTTCCTCTGTGGAATCCCTAGGTATAGACATTTTACTCCTAAAGGAAATCCCCTCTCTAGGGTTTCTCAAGTGCTGGTTGCAGCTACTAGGAAATGGAAGGTTCAGATTATGCCTAATTCTGAGGGATTGTTTGATGAGGATGAGAAGGATTTGGTTAGTAACAATGGAGCTAGGAAAATTCTACACACCAATGGATTCAGATTTTTGGACAGAGCAGCAGCAATTACATCACCTGAGATGGATCAATTTGAAGATGGAACTCGGAATCCATGGCGTCTCTGTACGGTTACTCAAGTCGAAGAAGTCAAATGCGTTCTCAGACTCCTCCCGATTTGGCTTTGCACGATCCTCTACTCCGTCGTCTTCACTCAAATGGCTTCTCTGTTCGTCGAGCAAGGCGCCGTCATGAGAACCAACATTTCTACATTCCACATCCCTCCCGCTAGCATGTCGAGCTTCGACATTCTCAGCGTCGCAGCGTTCATCTTCATCTACCGTAGAGTAATCGATCCAATCTACACTCGATTAACCAAATCGAGCCTCACGGAGCTTCAAAGAATGGGGATCGGACTTGTAATCGCAATCTGTGCCATGGTATCCGCCGGAACCGTCGAGGTTTTTCGATTAAGATACGCCGACAAAGATTGCCTCCATTGTGAGGAATCGAGTTCCCTAAGCATTTTCTGGCAGATTCCTCAGTACGTACTGATCGGAGCATCTGAGGTTTTCATGTACGTCGGCCAACTGGAATTCTTCAACAGTCAGGCACCTGACGGACTCAAAAGCTTCGGTAGTGCTCTCTGTATGACCTCGATTTCCTTCGGCAACTACGTTAGCAGCTTGCTGGTGACGATCGTGATGAAGGTCTCAACGAACGGTAACATGCCGGGATGGATCCCTGGAAACCTAAACAGAGGCCATTTAGACCGATTTTTCTTCCTCTTAGCCATCCTCACGGCGGCGGATCTCGTCGTCTACATCATCTGCGCCAAATGGTACAAATACATCAAATTCGAGAGCCGTGAGGCCGCCGCCGCCACCGCCACCGCCGCTGTCTAGGGCTTCGCTCCGACCGAAAATGGCGGCAGAATAAAGTTGGAGGTAGAAGAATGTCAATTCCAACGCCGTTTGATCCAATTTCGATGGTTCATAATCGAATTATTTGATTAGAAGAAAAATAAATAAATAAGTAGTTTTGAGGTGGTTCGATCGATGACCGTTGAAATTGGACCGGACATAATCGTGGCTGTAGATTTGAAGACGAAACCCACTCCGGTCGGAGGATAACCGGGCGGTTGACGCTATGAGACTTGAGAGATGATTCCGATTACTTCTCCAGTTGAGCGATGTAATATTTAATTCAACGTTTCATTGTTGTGTTATAATGAAATAAAAAATGTCATTTTTCTCTA

Coding sequence (CDS)

ATGGCTTGTCTCGATATCTCTTGTTTGCGTCAGAGTACTATGCAGAGGAAGAAGACGACGATGACGACGACAGCTGAGAAAGAGGTGCTTACGCTTGACGGAGCTGTTGACCGACATGGTCGTCCCGCTGTTCGGGGCAAAACTGGAACTTGGGTTGCTGGGATCTTGCTACTTGTGAACCAAGGGTTGGCTACGTTGGCGTTTTTTGGAGTTGGAGTGAACTTGGTGCTGTTTCTAACGAGGGTTATAGGACAAGACAATGCGGCAGCGGCCAATAACGTTAGCAAGTGGACAGGAACTGTCTACATTTTCTCTCTTCTTGGAGCCTTCCTTAGTGATTCGTACTTAGGAAGGTTCAAGACGTGTGCCATATTTCAAGCTATTTTCGTCTTGGGTCTAGCTTCACTATCAATAACATCCGGCCTATTTCTGTTGAAACCCAAAGGGTGTGGAGATGAGCATACTCCATGCGATTCTCACTCCTCCTACCACATTGCCCTGTTCTATCTCTCAGTATACTTGGTAGCCCTTGGCAATGGAGGATACCAACCCAACATAGCCACATTTGGTGCAGACCAGTTTGATGAAGAAGACCCTAAGGAAGGCCTCTCCAAAATTGCCTTCTTCAGCTACTTCTACTTGGCTCTCAACCTTGGCTCACTGTTCTCCAACACTATTTTGGGTTACTTTGAAGATGAAGGAATGTGGGTGATTGGTTTCTGGGCTTCTACTGCCTCTGCTGCTATGGCTTTGATCTTGTTCCTCTGTGGAATCCCTAGGTATAGACATTTTACTCCTAAAGGAAATCCCCTCTCTAGGGTTTCTCAAGTGCTGGTTGCAGCTACTAGGAAATGGAAGGTTCAGATTATGCCTAATTCTGAGGGATTGTTTGATGAGGATGAGAAGGATTTGGTTAGTAACAATGGAGCTAGGAAAATTCTACACACCAATGGATTCAGATTTTTGGACAGAGCAGCAGCAATTACATCACCTGAGATGGATCAATTTGAAGATGGAACTCGGAATCCATGGCGTCTCTGTACGGTTACTCAAGTCGAAGAAGTCAAATGCGTTCTCAGACTCCTCCCGATTTGGCTTTGCACGATCCTCTACTCCGTCGTCTTCACTCAAATGGCTTCTCTGTTCGTCGAGCAAGGCGCCGTCATGAGAACCAACATTTCTACATTCCACATCCCTCCCGCTAGCATGTCGAGCTTCGACATTCTCAGCGTCGCAGCGTTCATCTTCATCTACCGTAGAGTAATCGATCCAATCTACACTCGATTAACCAAATCGAGCCTCACGGAGCTTCAAAGAATGGGGATCGGACTTGTAATCGCAATCTGTGCCATGGTATCCGCCGGAACCGTCGAGGTTTTTCGATTAAGATACGCCGACAAAGATTGCCTCCATTGTGAGGAATCGAGTTCCCTAAGCATTTTCTGGCAGATTCCTCAGTACGTACTGATCGGAGCATCTGAGGTTTTCATGTACGTCGGCCAACTGGAATTCTTCAACAGTCAGGCACCTGACGGACTCAAAAGCTTCGGTAGTGCTCTCTGTATGACCTCGATTTCCTTCGGCAACTACGTTAGCAGCTTGCTGGTGACGATCGTGATGAAGGTCTCAACGAACGGTAACATGCCGGGATGGATCCCTGGAAACCTAAACAGAGGCCATTTAGACCGATTTTTCTTCCTCTTAGCCATCCTCACGGCGGCGGATCTCGTCGTCTACATCATCTGCGCCAAATGGTACAAATACATCAAATTCGAGAGCCGTGAGGCCGCCGCCGCCACCGCCACCGCCGCTGTCTAG

Protein sequence

MACLDISCLRQSTMQRKKTTMTTTAEKEVLTLDGAVDRHGRPAVRGKTGTWVAGILLLVNQGLATLAFFGVGVNLVLFLTRVIGQDNAAAANNVSKWTGTVYIFSLLGAFLSDSYLGRFKTCAIFQAIFVLGLASLSITSGLFLLKPKGCGDEHTPCDSHSSYHIALFYLSVYLVALGNGGYQPNIATFGADQFDEEDPKEGLSKIAFFSYFYLALNLGSLFSNTILGYFEDEGMWVIGFWASTASAAMALILFLCGIPRYRHFTPKGNPLSRVSQVLVAATRKWKVQIMPNSEGLFDEDEKDLVSNNGARKILHTNGFRFLDRAAAITSPEMDQFEDGTRNPWRLCTVTQVEEVKCVLRLLPIWLCTILYSVVFTQMASLFVEQGAVMRTNISTFHIPPASMSSFDILSVAAFIFIYRRVIDPIYTRLTKSSLTELQRMGIGLVIAICAMVSAGTVEVFRLRYADKDCLHCEESSSLSIFWQIPQYVLIGASEVFMYVGQLEFFNSQAPDGLKSFGSALCMTSISFGNYVSSLLVTIVMKVSTNGNMPGWIPGNLNRGHLDRFFFLLAILTAADLVVYIICAKWYKYIKFESREAAAATATAAV
Homology
BLAST of Carg14875 vs. NCBI nr
Match: KAG7018911.1 (Protein NRT1/ PTR FAMILY 7.3 [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 1198.7 bits (3100), Expect = 0.0e+00
Identity = 605/605 (100.00%), Postives = 605/605 (100.00%), Query Frame = 0

Query: 1   MACLDISCLRQSTMQRKKTTMTTTAEKEVLTLDGAVDRHGRPAVRGKTGTWVAGILLLVN 60
           MACLDISCLRQSTMQRKKTTMTTTAEKEVLTLDGAVDRHGRPAVRGKTGTWVAGILLLVN
Sbjct: 1   MACLDISCLRQSTMQRKKTTMTTTAEKEVLTLDGAVDRHGRPAVRGKTGTWVAGILLLVN 60

Query: 61  QGLATLAFFGVGVNLVLFLTRVIGQDNAAAANNVSKWTGTVYIFSLLGAFLSDSYLGRFK 120
           QGLATLAFFGVGVNLVLFLTRVIGQDNAAAANNVSKWTGTVYIFSLLGAFLSDSYLGRFK
Sbjct: 61  QGLATLAFFGVGVNLVLFLTRVIGQDNAAAANNVSKWTGTVYIFSLLGAFLSDSYLGRFK 120

Query: 121 TCAIFQAIFVLGLASLSITSGLFLLKPKGCGDEHTPCDSHSSYHIALFYLSVYLVALGNG 180
           TCAIFQAIFVLGLASLSITSGLFLLKPKGCGDEHTPCDSHSSYHIALFYLSVYLVALGNG
Sbjct: 121 TCAIFQAIFVLGLASLSITSGLFLLKPKGCGDEHTPCDSHSSYHIALFYLSVYLVALGNG 180

Query: 181 GYQPNIATFGADQFDEEDPKEGLSKIAFFSYFYLALNLGSLFSNTILGYFEDEGMWVIGF 240
           GYQPNIATFGADQFDEEDPKEGLSKIAFFSYFYLALNLGSLFSNTILGYFEDEGMWVIGF
Sbjct: 181 GYQPNIATFGADQFDEEDPKEGLSKIAFFSYFYLALNLGSLFSNTILGYFEDEGMWVIGF 240

Query: 241 WASTASAAMALILFLCGIPRYRHFTPKGNPLSRVSQVLVAATRKWKVQIMPNSEGLFDED 300
           WASTASAAMALILFLCGIPRYRHFTPKGNPLSRVSQVLVAATRKWKVQIMPNSEGLFDED
Sbjct: 241 WASTASAAMALILFLCGIPRYRHFTPKGNPLSRVSQVLVAATRKWKVQIMPNSEGLFDED 300

Query: 301 EKDLVSNNGARKILHTNGFRFLDRAAAITSPEMDQFEDGTRNPWRLCTVTQVEEVKCVLR 360
           EKDLVSNNGARKILHTNGFRFLDRAAAITSPEMDQFEDGTRNPWRLCTVTQVEEVKCVLR
Sbjct: 301 EKDLVSNNGARKILHTNGFRFLDRAAAITSPEMDQFEDGTRNPWRLCTVTQVEEVKCVLR 360

Query: 361 LLPIWLCTILYSVVFTQMASLFVEQGAVMRTNISTFHIPPASMSSFDILSVAAFIFIYRR 420
           LLPIWLCTILYSVVFTQMASLFVEQGAVMRTNISTFHIPPASMSSFDILSVAAFIFIYRR
Sbjct: 361 LLPIWLCTILYSVVFTQMASLFVEQGAVMRTNISTFHIPPASMSSFDILSVAAFIFIYRR 420

Query: 421 VIDPIYTRLTKSSLTELQRMGIGLVIAICAMVSAGTVEVFRLRYADKDCLHCEESSSLSI 480
           VIDPIYTRLTKSSLTELQRMGIGLVIAICAMVSAGTVEVFRLRYADKDCLHCEESSSLSI
Sbjct: 421 VIDPIYTRLTKSSLTELQRMGIGLVIAICAMVSAGTVEVFRLRYADKDCLHCEESSSLSI 480

Query: 481 FWQIPQYVLIGASEVFMYVGQLEFFNSQAPDGLKSFGSALCMTSISFGNYVSSLLVTIVM 540
           FWQIPQYVLIGASEVFMYVGQLEFFNSQAPDGLKSFGSALCMTSISFGNYVSSLLVTIVM
Sbjct: 481 FWQIPQYVLIGASEVFMYVGQLEFFNSQAPDGLKSFGSALCMTSISFGNYVSSLLVTIVM 540

Query: 541 KVSTNGNMPGWIPGNLNRGHLDRFFFLLAILTAADLVVYIICAKWYKYIKFESREAAAAT 600
           KVSTNGNMPGWIPGNLNRGHLDRFFFLLAILTAADLVVYIICAKWYKYIKFESREAAAAT
Sbjct: 541 KVSTNGNMPGWIPGNLNRGHLDRFFFLLAILTAADLVVYIICAKWYKYIKFESREAAAAT 600

Query: 601 ATAAV 606
           ATAAV
Sbjct: 601 ATAAV 605

BLAST of Carg14875 vs. NCBI nr
Match: KAG6582526.1 (Protein NRT1/ PTR FAMILY 7.3, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 1191.8 bits (3082), Expect = 0.0e+00
Identity = 601/605 (99.34%), Postives = 602/605 (99.50%), Query Frame = 0

Query: 1   MACLDISCLRQSTMQRKKTTMTTTAEKEVLTLDGAVDRHGRPAVRGKTGTWVAGILLLVN 60
           MACLDISCLRQSTMQRKKTTMTTTAEKEVLTLDGAVDRHGRPAVRGKTGTWVAGILLLVN
Sbjct: 1   MACLDISCLRQSTMQRKKTTMTTTAEKEVLTLDGAVDRHGRPAVRGKTGTWVAGILLLVN 60

Query: 61  QGLATLAFFGVGVNLVLFLTRVIGQDNAAAANNVSKWTGTVYIFSLLGAFLSDSYLGRFK 120
           QGLATLAFFGVGVNLVLFLTRVIGQDNAAAANNVSKWTGTVYIFSLLGAFLSDSYLGRFK
Sbjct: 61  QGLATLAFFGVGVNLVLFLTRVIGQDNAAAANNVSKWTGTVYIFSLLGAFLSDSYLGRFK 120

Query: 121 TCAIFQAIFVLGLASLSITSGLFLLKPKGCGDEHTPCDSHSSYHIALFYLSVYLVALGNG 180
           TCAIFQAIFVLGLASLSITSGLFLLKPKGCGDEHTPCDSHSSYHIALFYLSVYLVALGNG
Sbjct: 121 TCAIFQAIFVLGLASLSITSGLFLLKPKGCGDEHTPCDSHSSYHIALFYLSVYLVALGNG 180

Query: 181 GYQPNIATFGADQFDEEDPKEGLSKIAFFSYFYLALNLGSLFSNTILGYFEDEGMWVIGF 240
           GYQPNIATFGADQFDEEDPKEGLSKIAFFSYFYLALNLGSLFSNTILGYFEDEGMWVIGF
Sbjct: 181 GYQPNIATFGADQFDEEDPKEGLSKIAFFSYFYLALNLGSLFSNTILGYFEDEGMWVIGF 240

Query: 241 WASTASAAMALILFLCGIPRYRHFTPKGNPLSRVSQVLVAATRKWKVQIMPNSEGLFDED 300
           WASTASAAMALILFLCGIPRYRHFTPKGNPLSRVSQVL AATRKWKVQIMPNSEGLFDED
Sbjct: 241 WASTASAAMALILFLCGIPRYRHFTPKGNPLSRVSQVLAAATRKWKVQIMPNSEGLFDED 300

Query: 301 EKDLVSNNGARKILHTNGFRFLDRAAAITSPEMDQFEDGTRNPWRLCTVTQVEEVKCVLR 360
           EKDLVSNNGARKILHTNGFRFLDRAAAIT PEMDQF+DGTRNPWRLCTVTQVEEVKCVLR
Sbjct: 301 EKDLVSNNGARKILHTNGFRFLDRAAAITLPEMDQFDDGTRNPWRLCTVTQVEEVKCVLR 360

Query: 361 LLPIWLCTILYSVVFTQMASLFVEQGAVMRTNISTFHIPPASMSSFDILSVAAFIFIYRR 420
           LLPIWLCTILYSVVFTQMASLFVEQGAVMRTNISTFHIPPASMSSFDILSVAAFIFIYRR
Sbjct: 361 LLPIWLCTILYSVVFTQMASLFVEQGAVMRTNISTFHIPPASMSSFDILSVAAFIFIYRR 420

Query: 421 VIDPIYTRLTKSSLTELQRMGIGLVIAICAMVSAGTVEVFRLRYADKDCLHCEESSSLSI 480
           VIDPIYTRLTKSSLTELQRMGIGLVIAICAMVSAGTVEVFRLRYADKDCLHCEESSSLSI
Sbjct: 421 VIDPIYTRLTKSSLTELQRMGIGLVIAICAMVSAGTVEVFRLRYADKDCLHCEESSSLSI 480

Query: 481 FWQIPQYVLIGASEVFMYVGQLEFFNSQAPDGLKSFGSALCMTSISFGNYVSSLLVTIVM 540
           FWQIPQYVLIGASEVFMYVGQLEFFNSQAPDGLKSFGSALCMTSISFGNYVSSLLVTIVM
Sbjct: 481 FWQIPQYVLIGASEVFMYVGQLEFFNSQAPDGLKSFGSALCMTSISFGNYVSSLLVTIVM 540

Query: 541 KVSTNGNMPGWIPGNLNRGHLDRFFFLLAILTAADLVVYIICAKWYKYIKFESREAAAAT 600
           KVSTNGNMPGWIPGNLNRGHLDRFFFLLAILTAADLVVYIICAKWYKYIKFESREAAAA 
Sbjct: 541 KVSTNGNMPGWIPGNLNRGHLDRFFFLLAILTAADLVVYIICAKWYKYIKFESREAAAAA 600

Query: 601 ATAAV 606
           ATAAV
Sbjct: 601 ATAAV 605

BLAST of Carg14875 vs. NCBI nr
Match: XP_023526114.1 (protein NRT1/ PTR FAMILY 7.3-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 1182.5 bits (3058), Expect = 0.0e+00
Identity = 596/605 (98.51%), Postives = 599/605 (99.01%), Query Frame = 0

Query: 1   MACLDISCLRQSTMQRKKTTMTTTAEKEVLTLDGAVDRHGRPAVRGKTGTWVAGILLLVN 60
           MACL+ISCLRQSTMQRKKTT  TTAEKEVLTLDGAVDRHGRPAVRGKTGTWVAGILLLVN
Sbjct: 1   MACLNISCLRQSTMQRKKTTTMTTAEKEVLTLDGAVDRHGRPAVRGKTGTWVAGILLLVN 60

Query: 61  QGLATLAFFGVGVNLVLFLTRVIGQDNAAAANNVSKWTGTVYIFSLLGAFLSDSYLGRFK 120
           QGLATLAFFGVGVNLVLFLTRVIGQDNAAAANNVSKWTGTVYIFSLLGAFLSDSYLGRFK
Sbjct: 61  QGLATLAFFGVGVNLVLFLTRVIGQDNAAAANNVSKWTGTVYIFSLLGAFLSDSYLGRFK 120

Query: 121 TCAIFQAIFVLGLASLSITSGLFLLKPKGCGDEHTPCDSHSSYHIALFYLSVYLVALGNG 180
           TCAIFQAIFVLGLASLSITSGLFLLKPKGCGDEHTPCDSHSSYHIALFYLSVYLVALGNG
Sbjct: 121 TCAIFQAIFVLGLASLSITSGLFLLKPKGCGDEHTPCDSHSSYHIALFYLSVYLVALGNG 180

Query: 181 GYQPNIATFGADQFDEEDPKEGLSKIAFFSYFYLALNLGSLFSNTILGYFEDEGMWVIGF 240
           GYQPNIATFGADQFDEEDPKEGLSKIAFFSYFYLALNLGSLFSNTILGYFEDEGMWVIGF
Sbjct: 181 GYQPNIATFGADQFDEEDPKEGLSKIAFFSYFYLALNLGSLFSNTILGYFEDEGMWVIGF 240

Query: 241 WASTASAAMALILFLCGIPRYRHFTPKGNPLSRVSQVLVAATRKWKVQIMPNSEGLFDED 300
           WASTASAAMALILFLCGIPRYRHFTPKGNPLSRVSQVLVAATRKWKVQIMPNSEGLFDED
Sbjct: 241 WASTASAAMALILFLCGIPRYRHFTPKGNPLSRVSQVLVAATRKWKVQIMPNSEGLFDED 300

Query: 301 EKDLVSNNGARKILHTNGFRFLDRAAAITSPEMDQFEDGTRNPWRLCTVTQVEEVKCVLR 360
           EKDLVSNNGARKILHTNGFRFLDRAAAIT PEMDQF+DGTRNPWRLCTVTQVEEVKCVLR
Sbjct: 301 EKDLVSNNGARKILHTNGFRFLDRAAAITLPEMDQFDDGTRNPWRLCTVTQVEEVKCVLR 360

Query: 361 LLPIWLCTILYSVVFTQMASLFVEQGAVMRTNISTFHIPPASMSSFDILSVAAFIFIYRR 420
           LLPIWLCTILYSVVFTQMASLFVEQGAVMRTNISTFHIPPASMSSFDILSVAAFIFIYRR
Sbjct: 361 LLPIWLCTILYSVVFTQMASLFVEQGAVMRTNISTFHIPPASMSSFDILSVAAFIFIYRR 420

Query: 421 VIDPIYTRLTKSSLTELQRMGIGLVIAICAMVSAGTVEVFRLRYADKDCLHCEESSSLSI 480
           VIDPIYTRLTKSSLTELQRMGIGLVIAICAMVSAGTVEVFRLRYADKDCLHCEESSSLSI
Sbjct: 421 VIDPIYTRLTKSSLTELQRMGIGLVIAICAMVSAGTVEVFRLRYADKDCLHCEESSSLSI 480

Query: 481 FWQIPQYVLIGASEVFMYVGQLEFFNSQAPDGLKSFGSALCMTSISFGNYVSSLLVTIVM 540
           FWQIPQYVLIGASEVFMYVGQLEFFNSQAPDGLKSFGSALCMTSISFGNYVSSLLVTIVM
Sbjct: 481 FWQIPQYVLIGASEVFMYVGQLEFFNSQAPDGLKSFGSALCMTSISFGNYVSSLLVTIVM 540

Query: 541 KVSTNGNMPGWIPGNLNRGHLDRFFFLLAILTAADLVVYIICAKWYKYIKFESREAAAAT 600
           KVSTNGNMPGWIPGNLNRGHLDRFFFLLAILTAADL+VYIICAKWYKYIKFESREAAAA 
Sbjct: 541 KVSTNGNMPGWIPGNLNRGHLDRFFFLLAILTAADLIVYIICAKWYKYIKFESREAAAAA 600

Query: 601 ATAAV 606
           A A V
Sbjct: 601 AIATV 605

BLAST of Carg14875 vs. NCBI nr
Match: XP_022924814.1 (protein NRT1/ PTR FAMILY 7.3-like isoform X1 [Cucurbita moschata])

HSP 1 Score: 1181.0 bits (3054), Expect = 0.0e+00
Identity = 600/605 (99.17%), Postives = 601/605 (99.34%), Query Frame = 0

Query: 1   MACLDISCLRQSTMQRKKTTMTTTAEKEVLTLDGAVDRHGRPAVRGKTGTWVAGILLLVN 60
           MACLDISCLRQSTMQRKKTTM TTAEKEVLTLDGAVDRHGRPAVRGKTGTWVAGILLLVN
Sbjct: 1   MACLDISCLRQSTMQRKKTTM-TTAEKEVLTLDGAVDRHGRPAVRGKTGTWVAGILLLVN 60

Query: 61  QGLATLAFFGVGVNLVLFLTRVIGQDNAAAANNVSKWTGTVYIFSLLGAFLSDSYLGRFK 120
           QGLATLAFFGVGVNLVLFLTRVIGQDNAAAANNVSKWTGTVYIFSLLGAFLSDSYLGRFK
Sbjct: 61  QGLATLAFFGVGVNLVLFLTRVIGQDNAAAANNVSKWTGTVYIFSLLGAFLSDSYLGRFK 120

Query: 121 TCAIFQAIFVLGLASLSITSGLFLLKPKGCGDEHTPCDSHSSYHIALFYLSVYLVALGNG 180
           TCAIFQAIFVLGLASLSITSGLFLLKPKGCGDEHTPCDSHSSYHIALFYLSVYLVALGNG
Sbjct: 121 TCAIFQAIFVLGLASLSITSGLFLLKPKGCGDEHTPCDSHSSYHIALFYLSVYLVALGNG 180

Query: 181 GYQPNIATFGADQFDEEDPKEGLSKIAFFSYFYLALNLGSLFSNTILGYFEDEGMWVIGF 240
           GYQPNIATFGADQFDEEDPKEGLSKIAFFSYFYLALNLGSLFSNTILGYFEDEGMWVIGF
Sbjct: 181 GYQPNIATFGADQFDEEDPKEGLSKIAFFSYFYLALNLGSLFSNTILGYFEDEGMWVIGF 240

Query: 241 WASTASAAMALILFLCGIPRYRHFTPKGNPLSRVSQVLVAATRKWKVQIMPNSEGLFDED 300
           WASTASAAMALILFLCGIPRYRHFTPKGNPLSRVSQVLVAATRKWKVQIMPNSEGLFDED
Sbjct: 241 WASTASAAMALILFLCGIPRYRHFTPKGNPLSRVSQVLVAATRKWKVQIMPNSEGLFDED 300

Query: 301 EKDLVSNNGARKILHTNGFRFLDRAAAITSPEMDQFEDGTRNPWRLCTVTQVEEVKCVLR 360
           EKDLVSNNGARKILHTNGFRFLDRAAAIT PEMDQF+DGTRNPWRLCTVTQVEEVKCVLR
Sbjct: 301 EKDLVSNNGARKILHTNGFRFLDRAAAITLPEMDQFDDGTRNPWRLCTVTQVEEVKCVLR 360

Query: 361 LLPIWLCTILYSVVFTQMASLFVEQGAVMRTNISTFHIPPASMSSFDILSVAAFIFIYRR 420
           LLPIWLCTILYSVVFTQMASLFVEQGAVMRTNISTFHIPPASMSSFDILSVAAFIFIYRR
Sbjct: 361 LLPIWLCTILYSVVFTQMASLFVEQGAVMRTNISTFHIPPASMSSFDILSVAAFIFIYRR 420

Query: 421 VIDPIYTRLTKSSLTELQRMGIGLVIAICAMVSAGTVEVFRLRYADKDCLHCEESSSLSI 480
           VIDPIYTRLTKSSLTELQRMGIGLVIAICAMVSAGTVEVFRLRYADKDCLHCEESSSLSI
Sbjct: 421 VIDPIYTRLTKSSLTELQRMGIGLVIAICAMVSAGTVEVFRLRYADKDCLHCEESSSLSI 480

Query: 481 FWQIPQYVLIGASEVFMYVGQLEFFNSQAPDGLKSFGSALCMTSISFGNYVSSLLVTIVM 540
           FWQIPQYVLIGASEVFMYVGQLEFFNSQAPDGLKSFGSALCMTSISFGNYVSSLLVTIVM
Sbjct: 481 FWQIPQYVLIGASEVFMYVGQLEFFNSQAPDGLKSFGSALCMTSISFGNYVSSLLVTIVM 540

Query: 541 KVSTNGNMPGWIPGNLNRGHLDRFFFLLAILTAADLVVYIICAKWYKYIKFESREAAAAT 600
           KVSTNGNMPGWIPGNLNRGHLDRFFFLLAILTAADLVVYIICAKWYKYIKFESREAAAA 
Sbjct: 541 KVSTNGNMPGWIPGNLNRGHLDRFFFLLAILTAADLVVYIICAKWYKYIKFESREAAAA- 600

Query: 601 ATAAV 606
           ATA V
Sbjct: 601 ATATV 603

BLAST of Carg14875 vs. NCBI nr
Match: XP_022980257.1 (protein NRT1/ PTR FAMILY 7.3-like [Cucurbita maxima])

HSP 1 Score: 1172.1 bits (3031), Expect = 0.0e+00
Identity = 592/604 (98.01%), Postives = 597/604 (98.84%), Query Frame = 0

Query: 1   MACLDISCLRQSTMQRKKTTMTTTAEKEVLTLDGAVDRHGRPAVRGKTGTWVAGILLLVN 60
           MACL+ISC RQSTM+R+K TM TTAEKEVLTLDGAVDRHGRPAVRGKTGTWVAGILLLVN
Sbjct: 1   MACLNISCFRQSTMERRKKTM-TTAEKEVLTLDGAVDRHGRPAVRGKTGTWVAGILLLVN 60

Query: 61  QGLATLAFFGVGVNLVLFLTRVIGQDNAAAANNVSKWTGTVYIFSLLGAFLSDSYLGRFK 120
           QGLATLAFFGVGVNLVLFLTRVIGQDNAAAANNVSKWTGTVYIFSLLGAFLSDSYLGRFK
Sbjct: 61  QGLATLAFFGVGVNLVLFLTRVIGQDNAAAANNVSKWTGTVYIFSLLGAFLSDSYLGRFK 120

Query: 121 TCAIFQAIFVLGLASLSITSGLFLLKPKGCGDEHTPCDSHSSYHIALFYLSVYLVALGNG 180
           TCAIFQAIFVLGLASLSITS LFLLKPKGCGDEHTPCDSHSS+HIALFYLSVYLVALGNG
Sbjct: 121 TCAIFQAIFVLGLASLSITSDLFLLKPKGCGDEHTPCDSHSSFHIALFYLSVYLVALGNG 180

Query: 181 GYQPNIATFGADQFDEEDPKEGLSKIAFFSYFYLALNLGSLFSNTILGYFEDEGMWVIGF 240
           GYQPNIATFGADQFDEEDPKEGLSKIAFFSYFYLALNLGSLFSNTILGYFEDEGMWVIGF
Sbjct: 181 GYQPNIATFGADQFDEEDPKEGLSKIAFFSYFYLALNLGSLFSNTILGYFEDEGMWVIGF 240

Query: 241 WASTASAAMALILFLCGIPRYRHFTPKGNPLSRVSQVLVAATRKWKVQIMPNSEGLFDED 300
           WASTASAAMALILFLCGIPRYRHFTPKGNPLSRVSQVLVAATRKWKVQIMPNSEGLFDED
Sbjct: 241 WASTASAAMALILFLCGIPRYRHFTPKGNPLSRVSQVLVAATRKWKVQIMPNSEGLFDED 300

Query: 301 EKDLVSNNGARKILHTNGFRFLDRAAAITSPEMDQFEDGTRNPWRLCTVTQVEEVKCVLR 360
           EKDLVSNNGARKILHTNGFRFLDRAAAITSPEMDQF+DGTRNPWRLCTVTQVEEVKCVLR
Sbjct: 301 EKDLVSNNGARKILHTNGFRFLDRAAAITSPEMDQFDDGTRNPWRLCTVTQVEEVKCVLR 360

Query: 361 LLPIWLCTILYSVVFTQMASLFVEQGAVMRTNISTFHIPPASMSSFDILSVAAFIFIYRR 420
           LLPIWLCTILYSVVFTQMASLFVEQGAVMRTNISTFHIPPASMSSFDILSVAAFIFIYRR
Sbjct: 361 LLPIWLCTILYSVVFTQMASLFVEQGAVMRTNISTFHIPPASMSSFDILSVAAFIFIYRR 420

Query: 421 VIDPIYTRLTKSSLTELQRMGIGLVIAICAMVSAGTVEVFRLRYADKDCLHCEESSSLSI 480
           VIDPIYTRLTKSSLTELQRMGIGLVIAICAMVSAGTVEVFRLRYADKDCLHCEESSSLSI
Sbjct: 421 VIDPIYTRLTKSSLTELQRMGIGLVIAICAMVSAGTVEVFRLRYADKDCLHCEESSSLSI 480

Query: 481 FWQIPQYVLIGASEVFMYVGQLEFFNSQAPDGLKSFGSALCMTSISFGNYVSSLLVTIVM 540
           FWQIPQYVLIGASEVFMYVGQLEFFNSQAPDGLKSFGSALCMTSISFGNYVSSLLVTIVM
Sbjct: 481 FWQIPQYVLIGASEVFMYVGQLEFFNSQAPDGLKSFGSALCMTSISFGNYVSSLLVTIVM 540

Query: 541 KVSTNGNMPGWIPGNLNRGHLDRFFFLLAILTAADLVVYIICAKWYKYIKFESREAAAAT 600
           KVSTNGNMPGWIPGNLNRGHLDRFFFLLAILTAADLVVYIICAKWYKYIKFESREAAAA 
Sbjct: 541 KVSTNGNMPGWIPGNLNRGHLDRFFFLLAILTAADLVVYIICAKWYKYIKFESREAAAAA 600

Query: 601 ATAA 605
           A  A
Sbjct: 601 AATA 603

BLAST of Carg14875 vs. ExPASy Swiss-Prot
Match: Q9LQL2 (Protein NRT1/ PTR FAMILY 7.3 OS=Arabidopsis thaliana OX=3702 GN=NPF7.3 PE=1 SV=2)

HSP 1 Score: 831.2 bits (2146), Expect = 7.3e-240
Identity = 417/600 (69.50%), Postives = 492/600 (82.00%), Query Frame = 0

Query: 1   MACLDISCLRQSTMQRKKTTMTTTAEKEVLTLDGAVDRHGRPAVRGKTGTWVAGILLLVN 60
           M+CL+I    + TM++K        E E  T DG VD +GRP++R  +G WVAGI++L+N
Sbjct: 1   MSCLEI--YNKDTMKKK--------EGEEETRDGTVDYYGRPSIRSNSGQWVAGIVILLN 60

Query: 61  QGLATLAFFGVGVNLVLFLTRVIGQDNAAAANNVSKWTGTVYIFSLLGAFLSDSYLGRFK 120
           QGLATLAFFGVGVNLVLFLTRV+ Q+NA AANNVSKWTGTVYIFSL+GAFLSDSY GR+K
Sbjct: 61  QGLATLAFFGVGVNLVLFLTRVLQQNNADAANNVSKWTGTVYIFSLVGAFLSDSYWGRYK 120

Query: 121 TCAIFQAIFVLGLASLSITSGLFLLKPKGCGDEHTPCDSHSSYHIALFYLSVYLVALGNG 180
           TCAIFQ IFV+GL+SLS++S +FL++P+GCGDE TPC SHS   I +FY S+YL+ALG G
Sbjct: 121 TCAIFQVIFVIGLSSLSLSSYMFLIRPRGCGDEVTPCGSHSMMEITMFYFSIYLIALGYG 180

Query: 181 GYQPNIATFGADQFDEEDPKEGLSKIAFFSYFYLALNLGSLFSNTILGYFEDEGMWVIGF 240
           GYQPNIAT GADQFDEE PKEG SKIAFFSYFYLALNLGSLFSNTILGYFEDEGMW +GF
Sbjct: 181 GYQPNIATLGADQFDEEHPKEGYSKIAFFSYFYLALNLGSLFSNTILGYFEDEGMWALGF 240

Query: 241 WASTASAAMALILFLCGIPRYRHFTPKGNPLSRVSQVLVAATRKWKVQI-MPNSEGLFDE 300
           WAST SA + LILFL G PRYR+F P GNPLSR  QVLVAAT+K  V+  +   E ++D 
Sbjct: 241 WASTGSAIIGLILFLVGTPRYRYFKPTGNPLSRFCQVLVAATKKSSVEAPLRGREEMYDG 300

Query: 301 D-EKDLVSNNGARKILHTNGFRFLDRAAAITSPEMDQFEDGTRNPWRLCTVTQVEEVKCV 360
           D E    S N  R+I+HT+ F+FLD+AA IT+ ++D  +  + NPWRLC VTQVEEVKC+
Sbjct: 301 DSEGKNASVNTGRRIVHTDEFKFLDKAAYITARDLDDKKQDSVNPWRLCPVTQVEEVKCI 360

Query: 361 LRLLPIWLCTILYSVVFTQMASLFVEQGAVMRTNISTFHIPPASMSSFDILSVAAFIFIY 420
           LRL+PIWLCTI+YSVVFTQMASLFVEQGA M T++S F IPPASMSSFDILSVA FIF+Y
Sbjct: 361 LRLMPIWLCTIIYSVVFTQMASLFVEQGAAMNTSVSDFKIPPASMSSFDILSVALFIFLY 420

Query: 421 RRVIDPIYTRLTKS---SLTELQRMGIGLVIAICAMVSAGTVEVFRLRYADKDCLHCEES 480
           RRV++P+  R  K+    +TEL RMGIGLVIA+ AM++AG VE +RL+YADK C HC+ S
Sbjct: 421 RRVLEPVANRFKKNGSKGITELHRMGIGLVIAVIAMIAAGIVECYRLKYADKSCTHCDGS 480

Query: 481 SSLSIFWQIPQYVLIGASEVFMYVGQLEFFNSQAPDGLKSFGSALCMTSISFGNYVSSLL 540
           SSLSIFWQ PQY LIGASEVFMYVGQLEFFN+Q PDGLKSFGSALCM S+S GN+VSSLL
Sbjct: 481 SSLSIFWQAPQYSLIGASEVFMYVGQLEFFNAQTPDGLKSFGSALCMMSMSMGNFVSSLL 540

Query: 541 VTIVMKVSTNGNMPGWIPGNLNRGHLDRFFFLLAILTAADLVVYIICAKWYKYIKFESRE 596
           VT+V+K+ST  +MPGWIP NLN+GHLDRF+FLLA LT+ DLVVYI CAKWYK I+ E ++
Sbjct: 541 VTMVVKISTEDHMPGWIPRNLNKGHLDRFYFLLAALTSIDLVVYIACAKWYKPIQLEGKD 590

BLAST of Carg14875 vs. ExPASy Swiss-Prot
Match: Q8GXN2 (Protein NRT1/ PTR FAMILY 7.2 OS=Arabidopsis thaliana OX=3702 GN=NPF7.2 PE=2 SV=2)

HSP 1 Score: 800.0 bits (2065), Expect = 1.8e-230
Identity = 409/580 (70.52%), Postives = 467/580 (80.52%), Query Frame = 0

Query: 28  EVLTLDGAVDRHGRPAVRGKTGTWVAGILLLVNQGLATLAFFGVGVNLVLFLTRVIGQDN 87
           EV T DG+VDRHG PA+R  TG W+  IL+LVNQGLATLAFFGVGVNLVLFLTRV+GQDN
Sbjct: 9   EVCTQDGSVDRHGNPAIRANTGKWLTAILILVNQGLATLAFFGVGVNLVLFLTRVMGQDN 68

Query: 88  AAAANNVSKWTGTVYIFSLLGAFLSDSYLGRFKTCAIFQAIFVLGLASLSITSGLFLLKP 147
           A AANNVSKWTGTVYIFSLLGAFLSDSY GR+KTCAIFQA FV GL  LS+++G  LL+P
Sbjct: 69  AEAANNVSKWTGTVYIFSLLGAFLSDSYWGRYKTCAIFQASFVAGLMMLSLSTGALLLEP 128

Query: 148 KGCGDEHTPCDSHSSYHIALFYLSVYLVALGNGGYQPNIATFGADQFDEEDPKEGLSKIA 207
            GCG E +PC  HS++   LFYLSVYL+ALG GGYQPNIATFGADQFD ED  EG SKIA
Sbjct: 129 SGCGVEDSPCKPHSTFKTVLFYLSVYLIALGYGGYQPNIATFGADQFDAEDSVEGHSKIA 188

Query: 208 FFSYFYLALNLGSLFSNTILGYFEDEGMWVIGFWASTASAAMALILFLCGIPRYRHFTPK 267
           FFSYFYLALNLGSLFSNT+LGYFED+G W +GFWAS  SA   L+LFL G P+YRHFTP+
Sbjct: 189 FFSYFYLALNLGSLFSNTVLGYFEDQGEWPLGFWASAGSAFAGLVLFLIGTPKYRHFTPR 248

Query: 268 GNPLSRVSQVLVAATRKWKVQIMPNSEGLFDEDEKDLVSNNGARKILHTNGFRFLDRAAA 327
            +P SR  QVLVAATRK K+ +      L+D +        G +KILHT GFRFLDRAA 
Sbjct: 249 ESPWSRFCQVLVAATRKAKIDVHHEELNLYDSE----TQYTGDKKILHTKGFRFLDRAAI 308

Query: 328 IT-SPEMDQFEDGTR-NPWRLCTVTQVEEVKCVLRLLPIWLCTILYSVVFTQMASLFVEQ 387
           +T   E ++ E G++ +PWRLC+VTQVEEVKCVLRLLPIWLCTILYSVVFTQMASLFV Q
Sbjct: 309 VTPDDEAEKVESGSKYDPWRLCSVTQVEEVKCVLRLLPIWLCTILYSVVFTQMASLFVVQ 368

Query: 388 GAVMRTNISTFHIPPASMSSFDILSVAAFIFIYRRVIDPIYTRLTKS----SLTELQRMG 447
           GA M+TNI  F IP +SMSSFDILSVA FIF YRR +DP++ RL K+     LTELQRMG
Sbjct: 369 GAAMKTNIKNFRIPASSMSSFDILSVAFFIFAYRRFLDPLFARLNKTERNKGLTELQRMG 428

Query: 448 IGLVIAICAMVSAGTVEVFRLRYAD-KDCLHCEESSSLSIFWQIPQYVLIGASEVFMYVG 507
           IGLVIAI AM+SAG VE+ RL+  + +       SS+LSIFWQ+PQY+LIGASEVFMYVG
Sbjct: 429 IGLVIAIMAMISAGIVEIHRLKNKEPESATSISSSSTLSIFWQVPQYMLIGASEVFMYVG 488

Query: 508 QLEFFNSQAPDGLKSFGSALCMTSISFGNYVSSLLVTIVMKVSTNGNMPGWIPGNLNRGH 567
           QLEFFNSQAP GLKSF SALCM SIS GNYVSSLLV+IVMK+ST  ++ GWIP NLN+GH
Sbjct: 489 QLEFFNSQAPTGLKSFASALCMASISLGNYVSSLLVSIVMKISTTDDVHGWIPENLNKGH 548

Query: 568 LDRFFFLLAILTAADLVVYIICAKWYKYIKFESREAAAAT 601
           L+RF+FLLA LTAAD VVY+ICAKWYKYIK E+  + + T
Sbjct: 549 LERFYFLLAGLTAADFVVYLICAKWYKYIKSEASFSESVT 584

BLAST of Carg14875 vs. ExPASy Swiss-Prot
Match: Q3E9B5 (Protein NRT1/ PTR FAMILY 7.1 OS=Arabidopsis thaliana OX=3702 GN=NPF7.1 PE=2 SV=1)

HSP 1 Score: 634.8 bits (1636), Expect = 1.0e-180
Identity = 339/548 (61.86%), Postives = 398/548 (72.63%), Query Frame = 0

Query: 47  KTGTWVAGILLLVNQGLATLAFFGVGVNLVLFLTRVIGQDNAAAANNVSKWTGTVYIFSL 106
           K G W   I+LLVNQGLATLAFFGVGVNLVLFLTRV+GQ NA AANNVSKWTGTVY+FSL
Sbjct: 59  KNGGWTNAIILLVNQGLATLAFFGVGVNLVLFLTRVMGQGNAEAANNVSKWTGTVYMFSL 118

Query: 107 LGAFLSDSYLGRFKTCAIFQAIFVLGLASLSITSGLFLLKPKGCGDEHTPCDSHSSYHIA 166
           +GAFLSDSY GR+ TC IFQ IFV+G+  LS  S  FL+KP+GCGD    C+  SS  +A
Sbjct: 119 VGAFLSDSYWGRYLTCTIFQVIFVIGVGLLSFVSWFFLIKPRGCGDGDLECNPPSSLGVA 178

Query: 167 LFYLSVYLVALGNGGYQPNIATFGADQFDEEDPKEGLSKIAFFSYFYLALNLGSLFSNTI 226
           +FYLSVYLVA G GG+QP +ATFGADQ D++      SK AFFSYFY ALN+G+LFSNTI
Sbjct: 179 IFYLSVYLVAFGYGGHQPTLATFGADQLDDDKN----SKAAFFSYFYFALNVGALFSNTI 238

Query: 227 LGYFEDEGMWVIGFWASTASAAMALILFLCGIPRYRHFTPKGNPLSRVSQVLVAATRKWK 286
           L YFED+G+W  GF  S  SA +AL+ FL    +YR+  P GNPL RV+QV VA  RKW 
Sbjct: 239 LVYFEDKGLWTEGFLVSLGSAIVALVAFLAPTRQYRYVKPCGNPLPRVAQVFVATARKWS 298

Query: 287 VQIMPNSEGLFDEDEKDLVSNNGARKILHTNGFRFLDRAAAITSPEMDQFEDGTR-NPWR 346
           V + P       E E    +  G+RKI H+  F FLDRAA IT  +     +GTR N WR
Sbjct: 299 V-VRPGDPHELYELEGPESAIKGSRKIFHSTKFLFLDRAAVITEND----RNGTRSNAWR 358

Query: 347 LCTVTQVEEVKCVLRLLPIWLCTILYSVVFTQMASLFVEQGAVMRTNISTFHIPPASMSS 406
           LC+VTQVEE KCV++LLPIWLCTI+YSV+FTQMASLFVEQG VM   +  FHIP ASMS 
Sbjct: 359 LCSVTQVEEAKCVMKLLPIWLCTIIYSVIFTQMASLFVEQGDVMNAYVGKFHIPAASMSV 418

Query: 407 FDILSVAAFIFIYRRVIDPIYTRLTKSSLTELQRMGIGLVIAICAMVSAGTVEVFRLRYA 466
           FDI SV     IYR +I P Y R      TEL RMGIGL+I I AMV+AG  E+ RL   
Sbjct: 419 FDIFSVFVSTGIYRHIIFP-YVR-----PTELMRMGIGLIIGIMAMVAAGLTEIQRL--- 478

Query: 467 DKDCLHCEESSSLSIFWQIPQYVLIGASEVFMYVGQLEFFNSQAPDGLKSFGSALCMTSI 526
            K  +  ++ S L+I WQIPQYVL+GASEVFMYVGQLEFFN QAPDGLK+ GS+LCM S+
Sbjct: 479 -KRVVPGQKESELTILWQIPQYVLVGASEVFMYVGQLEFFNGQAPDGLKNLGSSLCMASM 538

Query: 527 SFGNYVSSLLVTIVMKVSTNG-NMPGWIPGNLNRGHLDRFFFLLAILTAADLVVYIICAK 586
           + GNYVSSL+V IVM ++  G N PGWIP NLN GH+DRF+FL+A L A D VVY+I AK
Sbjct: 539 ALGNYVSSLMVNIVMAITKRGENSPGWIPENLNEGHMDRFYFLIAALAAIDFVVYLIFAK 587

Query: 587 WYKYIKFE 593
           WY+ I  +
Sbjct: 599 WYQPISHD 587

BLAST of Carg14875 vs. ExPASy Swiss-Prot
Match: Q9M390 (Protein NRT1/ PTR FAMILY 8.1 OS=Arabidopsis thaliana OX=3702 GN=NPF8.1 PE=1 SV=1)

HSP 1 Score: 490.7 bits (1262), Expect = 2.3e-137
Identity = 256/572 (44.76%), Postives = 379/572 (66.26%), Query Frame = 0

Query: 26  EKEVLTLDGAVDRHGRPAVRGKTGTWVAGILLLVNQGLATLAFFGVGVNLVLFLTRVIGQ 85
           EK+V T DG VD H  PA + KTG W A   +L N+    LA++G+G NLV +L   + Q
Sbjct: 3   EKDVYTQDGTVDIHKNPANKEKTGNWKACRFILGNECCERLAYYGMGTNLVNYLESRLNQ 62

Query: 86  DNAAAANNVSKWTGTVYIFSLLGAFLSDSYLGRFKTCAIFQAIFVLGLASLSITSGLFLL 145
            NA AANNV+ W+GT YI  L+GAF++D+YLGR+ T A F  I+V G+  L++++ +  L
Sbjct: 63  GNATAANNVTNWSGTCYITPLIGAFIADAYLGRYWTIATFVFIYVSGMTLLTLSASVPGL 122

Query: 146 KPKGCGDEHTPCDSHSSYHIALFYLSVYLVALGNGGYQPNIATFGADQFDEEDPKEGLSK 205
           KP  C  +   C  +SS   A+F++++Y++ALG GG +P +++FGADQFDE D  E + K
Sbjct: 123 KPGNCNAD--TCHPNSS-QTAVFFVALYMIALGTGGIKPCVSSFGADQFDENDENEKIKK 182

Query: 206 IAFFSYFYLALNLGSLFSNTILGYFEDEGMWVIGFWASTASAAMALILFLCGIPRYRHFT 265
            +FF++FY ++N+G+L + T+L + +    W  GF   T +  +A+  F  G   YR   
Sbjct: 183 SSFFNWFYFSINVGALIAATVLVWIQMNVGWGWGFGVPTVAMVIAVCFFFFGSRFYRLQR 242

Query: 266 PKGNPLSRVSQVLVAATRKWKVQIMPNSEGLFDEDEKDLVSNNGARKILHTNGFRFLDRA 325
           P G+PL+R+ QV+VAA RK  V++ P  + L  E   D  +  G+RK++HT+  +F D+A
Sbjct: 243 PGGSPLTRIFQVIVAAFRKISVKV-PEDKSLLFETADDESNIKGSRKLVHTDNLKFFDKA 302

Query: 326 AAITSPEMDQFEDGTRNPWRLCTVTQVEEVKCVLRLLPIWLCTILYSVVFTQMASLFVEQ 385
           A     + D  +DG  NPWRLC+VTQVEE+K ++ LLP+W   I+++ V++QM+++FV Q
Sbjct: 303 A--VESQSDSIKDGEVNPWRLCSVTQVEELKSIITLLPVWATGIVFATVYSQMSTMFVLQ 362

Query: 386 GAVMRTNI-STFHIPPASMSSFDILSVAAFIFIYRRVIDPIYTRLTKS--SLTELQRMGI 445
           G  M  ++   F IP AS+S FD +SV  +  +Y + I P+  + T++    T+LQRMGI
Sbjct: 363 GNTMDQHMGKNFEIPSASLSLFDTVSVLFWTPVYDQFIIPLARKFTRNERGFTQLQRMGI 422

Query: 446 GLVIAICAMVSAGTVEVFRLRYADKDCLHCEESSSLSIFWQIPQYVLIGASEVFMYVGQL 505
           GLV++I AM++AG +EV RL Y      + ++   +SIFWQIPQY+LIG +EVF ++GQL
Sbjct: 423 GLVVSIFAMITAGVLEVVRLDYVKTHNAYDQKQIHMSIFWQIPQYLLIGCAEVFTFIGQL 482

Query: 506 EFFNSQAPDGLKSFGSALCMTSISFGNYVSSLLVTIVMKVSTNGNMPGWIPGNLNRGHLD 565
           EFF  QAPD ++S  SAL +T+++ GNY+S++LVT+VMK++     PGWIP NLNRGHLD
Sbjct: 483 EFFYDQAPDAMRSLCSALSLTTVALGNYLSTVLVTVVMKITKKNGKPGWIPDNLNRGHLD 542

Query: 566 RFFFLLAILTAADLVVYIICAKWYKYIKFESR 595
            FF+LLA L+  + +VY+  +K YKY K   R
Sbjct: 543 YFFYLLATLSFLNFLVYLWISKRYKYKKAVGR 568

BLAST of Carg14875 vs. ExPASy Swiss-Prot
Match: Q9LFB8 (Protein NRT1/ PTR FAMILY 8.2 OS=Arabidopsis thaliana OX=3702 GN=NPF8.2 PE=2 SV=1)

HSP 1 Score: 459.1 bits (1180), Expect = 7.5e-128
Identity = 246/568 (43.31%), Postives = 372/568 (65.49%), Query Frame = 0

Query: 26  EKEVLTLDGAVDRHGRPAVRGKTGTWVAGILLLVNQGLATLAFFGVGVNLVLFLTRVIGQ 85
           +K++ T DG +D H +PA + KTGTW A   +L  +    LA++G+  NL+ +L + +  
Sbjct: 4   DKDIYTKDGTLDIHKKPANKNKTGTWKACRFILGTECCERLAYYGMSTNLINYLEKQMNM 63

Query: 86  DNAAAANNVSKWTGTVYIFSLLGAFLSDSYLGRFKTCAIFQAIFVLGLASLSITSGLFLL 145
           +N +A+ +VS W+GT Y   L+GAF++D+YLGR+ T A F  I++ G+  L+I++ +  L
Sbjct: 64  ENVSASKSVSNWSGTCYATPLIGAFIADAYLGRYWTIASFVVIYIAGMTLLTISASVPGL 123

Query: 146 KPKGCGDEHTPCDSHSSYHIALFYLSVYLVALGNGGYQPNIATFGADQFDEEDPKEGLSK 205
            P   G+    C + ++   A+ ++++YL+ALG GG +P +++FGADQFD+ D KE  SK
Sbjct: 124 TPTCSGE---TCHA-TAGQTAITFIALYLIALGTGGIKPCVSSFGADQFDDTDEKEKESK 183

Query: 206 IAFFSYFYLALNLGSLFSNTILGYFEDEGMWVIGFWASTASAAMALILFLCGIPRYRHFT 265
            +FF++FY  +N+G++ ++++L + +    W  G    T + A+A++ F  G   YR   
Sbjct: 184 SSFFNWFYFVINVGAMIASSVLVWIQMNVGWGWGLGVPTVAMAIAVVFFFAGSNFYRLQK 243

Query: 266 PKGNPLSRVSQVLVAATRKWKVQIMPNSEGLFDEDEKDLVSNNGARKILHTNGFRFLDRA 325
           P G+PL+R+ QV+VA+ RK KV+I P  E L  E++    S  G+RK+ HT    F D+A
Sbjct: 244 PGGSPLTRMLQVIVASCRKSKVKI-PEDESLLYENQDAESSIIGSRKLEHTKILTFFDKA 303

Query: 326 AAITSPEMDQFEDGTRNPWRLCTVTQVEEVKCVLRLLPIWLCTILYSVVFTQMASLFVEQ 385
           A  T  E D       + W+LCTVTQVEE+K ++RLLPIW   I+++ V++QM ++FV Q
Sbjct: 304 AVET--ESDNKGAAKSSSWKLCTVTQVEELKALIRLLPIWATGIVFASVYSQMGTVFVLQ 363

Query: 386 GAVMRTNIS-TFHIPPASMSSFDILSVAAFIFIYRRVIDPIYTRLT--KSSLTELQRMGI 445
           G  +  ++   F IP AS+S FD LSV  +  +Y ++I P   + T  +   T+LQR+GI
Sbjct: 364 GNTLDQHMGPNFKIPSASLSLFDTLSVLFWAPVYDKLIVPFARKYTGHERGFTQLQRIGI 423

Query: 446 GLVIAICAMVSAGTVEVFRLRYADKDCLHCEESSSLSIFWQIPQYVLIGASEVFMYVGQL 505
           GLVI+I +MVSAG +EV RL Y     L+ EE+  ++IFWQ+PQY L+G +EVF ++GQL
Sbjct: 424 GLVISIFSMVSAGILEVARLNYVQTHNLYNEETIPMTIFWQVPQYFLVGCAEVFTFIGQL 483

Query: 506 EFFNSQAPDGLKSFGSALCMTSISFGNYVSSLLVTIVMKVSTNGNMPGWIPGNLNRGHLD 565
           EFF  QAPD ++S  SAL +T+I+FGNY+S+ LVT+V KV+ +G  PGWI  NLN GHLD
Sbjct: 484 EFFYDQAPDAMRSLCSALSLTAIAFGNYLSTFLVTLVTKVTRSGGRPGWIAKNLNNGHLD 543

Query: 566 RFFFLLAILTAADLVVYIICAKWYKYIK 591
            FF+LLA L+  + +VY+  AKWY Y K
Sbjct: 544 YFFWLLAGLSFLNFLVYLWIAKWYTYKK 564

BLAST of Carg14875 vs. ExPASy TrEMBL
Match: A0A6J1EA96 (protein NRT1/ PTR FAMILY 7.3-like isoform X1 OS=Cucurbita moschata OX=3662 GN=LOC111432194 PE=3 SV=1)

HSP 1 Score: 1181.0 bits (3054), Expect = 0.0e+00
Identity = 600/605 (99.17%), Postives = 601/605 (99.34%), Query Frame = 0

Query: 1   MACLDISCLRQSTMQRKKTTMTTTAEKEVLTLDGAVDRHGRPAVRGKTGTWVAGILLLVN 60
           MACLDISCLRQSTMQRKKTTM TTAEKEVLTLDGAVDRHGRPAVRGKTGTWVAGILLLVN
Sbjct: 1   MACLDISCLRQSTMQRKKTTM-TTAEKEVLTLDGAVDRHGRPAVRGKTGTWVAGILLLVN 60

Query: 61  QGLATLAFFGVGVNLVLFLTRVIGQDNAAAANNVSKWTGTVYIFSLLGAFLSDSYLGRFK 120
           QGLATLAFFGVGVNLVLFLTRVIGQDNAAAANNVSKWTGTVYIFSLLGAFLSDSYLGRFK
Sbjct: 61  QGLATLAFFGVGVNLVLFLTRVIGQDNAAAANNVSKWTGTVYIFSLLGAFLSDSYLGRFK 120

Query: 121 TCAIFQAIFVLGLASLSITSGLFLLKPKGCGDEHTPCDSHSSYHIALFYLSVYLVALGNG 180
           TCAIFQAIFVLGLASLSITSGLFLLKPKGCGDEHTPCDSHSSYHIALFYLSVYLVALGNG
Sbjct: 121 TCAIFQAIFVLGLASLSITSGLFLLKPKGCGDEHTPCDSHSSYHIALFYLSVYLVALGNG 180

Query: 181 GYQPNIATFGADQFDEEDPKEGLSKIAFFSYFYLALNLGSLFSNTILGYFEDEGMWVIGF 240
           GYQPNIATFGADQFDEEDPKEGLSKIAFFSYFYLALNLGSLFSNTILGYFEDEGMWVIGF
Sbjct: 181 GYQPNIATFGADQFDEEDPKEGLSKIAFFSYFYLALNLGSLFSNTILGYFEDEGMWVIGF 240

Query: 241 WASTASAAMALILFLCGIPRYRHFTPKGNPLSRVSQVLVAATRKWKVQIMPNSEGLFDED 300
           WASTASAAMALILFLCGIPRYRHFTPKGNPLSRVSQVLVAATRKWKVQIMPNSEGLFDED
Sbjct: 241 WASTASAAMALILFLCGIPRYRHFTPKGNPLSRVSQVLVAATRKWKVQIMPNSEGLFDED 300

Query: 301 EKDLVSNNGARKILHTNGFRFLDRAAAITSPEMDQFEDGTRNPWRLCTVTQVEEVKCVLR 360
           EKDLVSNNGARKILHTNGFRFLDRAAAIT PEMDQF+DGTRNPWRLCTVTQVEEVKCVLR
Sbjct: 301 EKDLVSNNGARKILHTNGFRFLDRAAAITLPEMDQFDDGTRNPWRLCTVTQVEEVKCVLR 360

Query: 361 LLPIWLCTILYSVVFTQMASLFVEQGAVMRTNISTFHIPPASMSSFDILSVAAFIFIYRR 420
           LLPIWLCTILYSVVFTQMASLFVEQGAVMRTNISTFHIPPASMSSFDILSVAAFIFIYRR
Sbjct: 361 LLPIWLCTILYSVVFTQMASLFVEQGAVMRTNISTFHIPPASMSSFDILSVAAFIFIYRR 420

Query: 421 VIDPIYTRLTKSSLTELQRMGIGLVIAICAMVSAGTVEVFRLRYADKDCLHCEESSSLSI 480
           VIDPIYTRLTKSSLTELQRMGIGLVIAICAMVSAGTVEVFRLRYADKDCLHCEESSSLSI
Sbjct: 421 VIDPIYTRLTKSSLTELQRMGIGLVIAICAMVSAGTVEVFRLRYADKDCLHCEESSSLSI 480

Query: 481 FWQIPQYVLIGASEVFMYVGQLEFFNSQAPDGLKSFGSALCMTSISFGNYVSSLLVTIVM 540
           FWQIPQYVLIGASEVFMYVGQLEFFNSQAPDGLKSFGSALCMTSISFGNYVSSLLVTIVM
Sbjct: 481 FWQIPQYVLIGASEVFMYVGQLEFFNSQAPDGLKSFGSALCMTSISFGNYVSSLLVTIVM 540

Query: 541 KVSTNGNMPGWIPGNLNRGHLDRFFFLLAILTAADLVVYIICAKWYKYIKFESREAAAAT 600
           KVSTNGNMPGWIPGNLNRGHLDRFFFLLAILTAADLVVYIICAKWYKYIKFESREAAAA 
Sbjct: 541 KVSTNGNMPGWIPGNLNRGHLDRFFFLLAILTAADLVVYIICAKWYKYIKFESREAAAA- 600

Query: 601 ATAAV 606
           ATA V
Sbjct: 601 ATATV 603

BLAST of Carg14875 vs. ExPASy TrEMBL
Match: A0A6J1IQW8 (protein NRT1/ PTR FAMILY 7.3-like OS=Cucurbita maxima OX=3661 GN=LOC111479679 PE=3 SV=1)

HSP 1 Score: 1172.1 bits (3031), Expect = 0.0e+00
Identity = 592/604 (98.01%), Postives = 597/604 (98.84%), Query Frame = 0

Query: 1   MACLDISCLRQSTMQRKKTTMTTTAEKEVLTLDGAVDRHGRPAVRGKTGTWVAGILLLVN 60
           MACL+ISC RQSTM+R+K TM TTAEKEVLTLDGAVDRHGRPAVRGKTGTWVAGILLLVN
Sbjct: 1   MACLNISCFRQSTMERRKKTM-TTAEKEVLTLDGAVDRHGRPAVRGKTGTWVAGILLLVN 60

Query: 61  QGLATLAFFGVGVNLVLFLTRVIGQDNAAAANNVSKWTGTVYIFSLLGAFLSDSYLGRFK 120
           QGLATLAFFGVGVNLVLFLTRVIGQDNAAAANNVSKWTGTVYIFSLLGAFLSDSYLGRFK
Sbjct: 61  QGLATLAFFGVGVNLVLFLTRVIGQDNAAAANNVSKWTGTVYIFSLLGAFLSDSYLGRFK 120

Query: 121 TCAIFQAIFVLGLASLSITSGLFLLKPKGCGDEHTPCDSHSSYHIALFYLSVYLVALGNG 180
           TCAIFQAIFVLGLASLSITS LFLLKPKGCGDEHTPCDSHSS+HIALFYLSVYLVALGNG
Sbjct: 121 TCAIFQAIFVLGLASLSITSDLFLLKPKGCGDEHTPCDSHSSFHIALFYLSVYLVALGNG 180

Query: 181 GYQPNIATFGADQFDEEDPKEGLSKIAFFSYFYLALNLGSLFSNTILGYFEDEGMWVIGF 240
           GYQPNIATFGADQFDEEDPKEGLSKIAFFSYFYLALNLGSLFSNTILGYFEDEGMWVIGF
Sbjct: 181 GYQPNIATFGADQFDEEDPKEGLSKIAFFSYFYLALNLGSLFSNTILGYFEDEGMWVIGF 240

Query: 241 WASTASAAMALILFLCGIPRYRHFTPKGNPLSRVSQVLVAATRKWKVQIMPNSEGLFDED 300
           WASTASAAMALILFLCGIPRYRHFTPKGNPLSRVSQVLVAATRKWKVQIMPNSEGLFDED
Sbjct: 241 WASTASAAMALILFLCGIPRYRHFTPKGNPLSRVSQVLVAATRKWKVQIMPNSEGLFDED 300

Query: 301 EKDLVSNNGARKILHTNGFRFLDRAAAITSPEMDQFEDGTRNPWRLCTVTQVEEVKCVLR 360
           EKDLVSNNGARKILHTNGFRFLDRAAAITSPEMDQF+DGTRNPWRLCTVTQVEEVKCVLR
Sbjct: 301 EKDLVSNNGARKILHTNGFRFLDRAAAITSPEMDQFDDGTRNPWRLCTVTQVEEVKCVLR 360

Query: 361 LLPIWLCTILYSVVFTQMASLFVEQGAVMRTNISTFHIPPASMSSFDILSVAAFIFIYRR 420
           LLPIWLCTILYSVVFTQMASLFVEQGAVMRTNISTFHIPPASMSSFDILSVAAFIFIYRR
Sbjct: 361 LLPIWLCTILYSVVFTQMASLFVEQGAVMRTNISTFHIPPASMSSFDILSVAAFIFIYRR 420

Query: 421 VIDPIYTRLTKSSLTELQRMGIGLVIAICAMVSAGTVEVFRLRYADKDCLHCEESSSLSI 480
           VIDPIYTRLTKSSLTELQRMGIGLVIAICAMVSAGTVEVFRLRYADKDCLHCEESSSLSI
Sbjct: 421 VIDPIYTRLTKSSLTELQRMGIGLVIAICAMVSAGTVEVFRLRYADKDCLHCEESSSLSI 480

Query: 481 FWQIPQYVLIGASEVFMYVGQLEFFNSQAPDGLKSFGSALCMTSISFGNYVSSLLVTIVM 540
           FWQIPQYVLIGASEVFMYVGQLEFFNSQAPDGLKSFGSALCMTSISFGNYVSSLLVTIVM
Sbjct: 481 FWQIPQYVLIGASEVFMYVGQLEFFNSQAPDGLKSFGSALCMTSISFGNYVSSLLVTIVM 540

Query: 541 KVSTNGNMPGWIPGNLNRGHLDRFFFLLAILTAADLVVYIICAKWYKYIKFESREAAAAT 600
           KVSTNGNMPGWIPGNLNRGHLDRFFFLLAILTAADLVVYIICAKWYKYIKFESREAAAA 
Sbjct: 541 KVSTNGNMPGWIPGNLNRGHLDRFFFLLAILTAADLVVYIICAKWYKYIKFESREAAAAA 600

Query: 601 ATAA 605
           A  A
Sbjct: 601 AATA 603

BLAST of Carg14875 vs. ExPASy TrEMBL
Match: A0A6J1EG49 (protein NRT1/ PTR FAMILY 7.3-like isoform X2 OS=Cucurbita moschata OX=3662 GN=LOC111432194 PE=3 SV=1)

HSP 1 Score: 1168.3 bits (3021), Expect = 0.0e+00
Identity = 596/605 (98.51%), Postives = 597/605 (98.68%), Query Frame = 0

Query: 1   MACLDISCLRQSTMQRKKTTMTTTAEKEVLTLDGAVDRHGRPAVRGKTGTWVAGILLLVN 60
           MACLDISCLR    QRKKTTM TTAEKEVLTLDGAVDRHGRPAVRGKTGTWVAGILLLVN
Sbjct: 1   MACLDISCLR----QRKKTTM-TTAEKEVLTLDGAVDRHGRPAVRGKTGTWVAGILLLVN 60

Query: 61  QGLATLAFFGVGVNLVLFLTRVIGQDNAAAANNVSKWTGTVYIFSLLGAFLSDSYLGRFK 120
           QGLATLAFFGVGVNLVLFLTRVIGQDNAAAANNVSKWTGTVYIFSLLGAFLSDSYLGRFK
Sbjct: 61  QGLATLAFFGVGVNLVLFLTRVIGQDNAAAANNVSKWTGTVYIFSLLGAFLSDSYLGRFK 120

Query: 121 TCAIFQAIFVLGLASLSITSGLFLLKPKGCGDEHTPCDSHSSYHIALFYLSVYLVALGNG 180
           TCAIFQAIFVLGLASLSITSGLFLLKPKGCGDEHTPCDSHSSYHIALFYLSVYLVALGNG
Sbjct: 121 TCAIFQAIFVLGLASLSITSGLFLLKPKGCGDEHTPCDSHSSYHIALFYLSVYLVALGNG 180

Query: 181 GYQPNIATFGADQFDEEDPKEGLSKIAFFSYFYLALNLGSLFSNTILGYFEDEGMWVIGF 240
           GYQPNIATFGADQFDEEDPKEGLSKIAFFSYFYLALNLGSLFSNTILGYFEDEGMWVIGF
Sbjct: 181 GYQPNIATFGADQFDEEDPKEGLSKIAFFSYFYLALNLGSLFSNTILGYFEDEGMWVIGF 240

Query: 241 WASTASAAMALILFLCGIPRYRHFTPKGNPLSRVSQVLVAATRKWKVQIMPNSEGLFDED 300
           WASTASAAMALILFLCGIPRYRHFTPKGNPLSRVSQVLVAATRKWKVQIMPNSEGLFDED
Sbjct: 241 WASTASAAMALILFLCGIPRYRHFTPKGNPLSRVSQVLVAATRKWKVQIMPNSEGLFDED 300

Query: 301 EKDLVSNNGARKILHTNGFRFLDRAAAITSPEMDQFEDGTRNPWRLCTVTQVEEVKCVLR 360
           EKDLVSNNGARKILHTNGFRFLDRAAAIT PEMDQF+DGTRNPWRLCTVTQVEEVKCVLR
Sbjct: 301 EKDLVSNNGARKILHTNGFRFLDRAAAITLPEMDQFDDGTRNPWRLCTVTQVEEVKCVLR 360

Query: 361 LLPIWLCTILYSVVFTQMASLFVEQGAVMRTNISTFHIPPASMSSFDILSVAAFIFIYRR 420
           LLPIWLCTILYSVVFTQMASLFVEQGAVMRTNISTFHIPPASMSSFDILSVAAFIFIYRR
Sbjct: 361 LLPIWLCTILYSVVFTQMASLFVEQGAVMRTNISTFHIPPASMSSFDILSVAAFIFIYRR 420

Query: 421 VIDPIYTRLTKSSLTELQRMGIGLVIAICAMVSAGTVEVFRLRYADKDCLHCEESSSLSI 480
           VIDPIYTRLTKSSLTELQRMGIGLVIAICAMVSAGTVEVFRLRYADKDCLHCEESSSLSI
Sbjct: 421 VIDPIYTRLTKSSLTELQRMGIGLVIAICAMVSAGTVEVFRLRYADKDCLHCEESSSLSI 480

Query: 481 FWQIPQYVLIGASEVFMYVGQLEFFNSQAPDGLKSFGSALCMTSISFGNYVSSLLVTIVM 540
           FWQIPQYVLIGASEVFMYVGQLEFFNSQAPDGLKSFGSALCMTSISFGNYVSSLLVTIVM
Sbjct: 481 FWQIPQYVLIGASEVFMYVGQLEFFNSQAPDGLKSFGSALCMTSISFGNYVSSLLVTIVM 540

Query: 541 KVSTNGNMPGWIPGNLNRGHLDRFFFLLAILTAADLVVYIICAKWYKYIKFESREAAAAT 600
           KVSTNGNMPGWIPGNLNRGHLDRFFFLLAILTAADLVVYIICAKWYKYIKFESREAAAA 
Sbjct: 541 KVSTNGNMPGWIPGNLNRGHLDRFFFLLAILTAADLVVYIICAKWYKYIKFESREAAAA- 599

Query: 601 ATAAV 606
           ATA V
Sbjct: 601 ATATV 599

BLAST of Carg14875 vs. ExPASy TrEMBL
Match: A0A0A0L6J7 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_3G127170 PE=3 SV=1)

HSP 1 Score: 1065.4 bits (2754), Expect = 8.4e-308
Identity = 528/601 (87.85%), Postives = 566/601 (94.18%), Query Frame = 0

Query: 1   MACLDISCLRQSTMQRKKTTMTTTAEKEVLTLDGAVDRHGRPAVRGKTGTWVAGILLLVN 60
           MACL ISC  QST ++K      T+EKEV TLDGAVDRHG PA+R KTGTWV+GIL+LVN
Sbjct: 1   MACLSISCFDQSTKKKK----VMTSEKEVRTLDGAVDRHGHPAIRDKTGTWVSGILILVN 60

Query: 61  QGLATLAFFGVGVNLVLFLTRVIGQDNAAAANNVSKWTGTVYIFSLLGAFLSDSYLGRFK 120
           QGLATLAFFGVGVNLVLFLTRV+GQDNA AANNVSKWTGTVYIFSLLGAFLSDSY GRFK
Sbjct: 61  QGLATLAFFGVGVNLVLFLTRVVGQDNAEAANNVSKWTGTVYIFSLLGAFLSDSYWGRFK 120

Query: 121 TCAIFQAIFVLGLASLSITSGLFLLKPKGCGDEHTPCDSHSSYHIALFYLSVYLVALGNG 180
           TCAIFQAIFV+GLASLSITS +FL++PKGCGDEHTPC+SHSS HIALFYLSVYLVALGNG
Sbjct: 121 TCAIFQAIFVVGLASLSITSYIFLVRPKGCGDEHTPCNSHSSLHIALFYLSVYLVALGNG 180

Query: 181 GYQPNIATFGADQFDEEDPKEGLSKIAFFSYFYLALNLGSLFSNTILGYFEDEGMWVIGF 240
           GYQPNIATFGADQFDEEDPKEG SKIAFFSYFYLALNLGSLFSNTILGYFEDEGMWV+GF
Sbjct: 181 GYQPNIATFGADQFDEEDPKEGQSKIAFFSYFYLALNLGSLFSNTILGYFEDEGMWVLGF 240

Query: 241 WASTASAAMALILFLCGIPRYRHFTPKGNPLSRVSQVLVAATRKWKVQIMPNSEGLFDED 300
           WASTASAA+AL+LFLCGIPRYRHFTPKGNPLSRVSQV++AATRKWKVQ++PNSEGLFD+D
Sbjct: 241 WASTASAALALLLFLCGIPRYRHFTPKGNPLSRVSQVVIAATRKWKVQMIPNSEGLFDDD 300

Query: 301 EKDLVSNNGARKILHTNGFRFLDRAAAITSPEMDQFEDGTRNPWRLCTVTQVEEVKCVLR 360
           +K+L S NGAR+ILHTNGF+FLD+AA ITS E DQ +DG RNPWRLCTVTQVEEVKC+LR
Sbjct: 301 DKELAS-NGARRILHTNGFKFLDKAAVITSSEYDQLDDGARNPWRLCTVTQVEEVKCILR 360

Query: 361 LLPIWLCTILYSVVFTQMASLFVEQGAVMRTNISTFHIPPASMSSFDILSVAAFIFIYRR 420
           LLPIWLCTILYSVVFTQMASLFV QGA MRTNISTFH+PPASMSSFDILSVAAFIFIYRR
Sbjct: 361 LLPIWLCTILYSVVFTQMASLFVVQGAAMRTNISTFHVPPASMSSFDILSVAAFIFIYRR 420

Query: 421 VIDPIYTRLTKSSLTELQRMGIGLVIAICAMVSAGTVEVFRLRYADKDCLHCEESSSLSI 480
           VIDP++ RLTKSSLTELQRMGIGLVIAICAMVSAGTVE+FRL++A+KDCL C+ SSSLSI
Sbjct: 421 VIDPVFARLTKSSLTELQRMGIGLVIAICAMVSAGTVEIFRLKHANKDCLRCDNSSSLSI 480

Query: 481 FWQIPQYVLIGASEVFMYVGQLEFFNSQAPDGLKSFGSALCMTSISFGNYVSSLLVTIVM 540
           FWQIPQYVLIGASEVFMYVGQLEFFNSQAPDGLKSFGSALCMTSISFGNYVSSLLVTIVM
Sbjct: 481 FWQIPQYVLIGASEVFMYVGQLEFFNSQAPDGLKSFGSALCMTSISFGNYVSSLLVTIVM 540

Query: 541 KVSTNGNMPGWIPGNLNRGHLDRFFFLLAILTAADLVVYIICAKWYKYIKFESREAAAAT 600
           K+S   NMPGWIPGNLNRGHLDRF+FLLA LTAADLVVYI+CAKWYKYIKFESREA AAT
Sbjct: 541 KISATDNMPGWIPGNLNRGHLDRFYFLLAALTAADLVVYILCAKWYKYIKFESREADAAT 596

Query: 601 A 602
           A
Sbjct: 601 A 596

BLAST of Carg14875 vs. ExPASy TrEMBL
Match: A0A6J1CZL9 (protein NRT1/ PTR FAMILY 7.3-like OS=Momordica charantia OX=3673 GN=LOC111016031 PE=3 SV=1)

HSP 1 Score: 1065.1 bits (2753), Expect = 1.1e-307
Identity = 531/599 (88.65%), Postives = 562/599 (93.82%), Query Frame = 0

Query: 1   MACLDISCLRQSTMQRKKTTMTTTAEKEVLTLDGAVDRHGRPAVRGKTGTWVAGILLLVN 60
           MACL+ SC RQ T+  K T   T +EKE  TLDGAVDRHG PA+R KTGTW AGIL+LVN
Sbjct: 1   MACLNTSCFRQ-TVGEKTTKKRTISEKETCTLDGAVDRHGHPAIRDKTGTWFAGILILVN 60

Query: 61  QGLATLAFFGVGVNLVLFLTRVIGQDNAAAANNVSKWTGTVYIFSLLGAFLSDSYLGRFK 120
           QGLATLAFFGVGVNLVLFLTRV+GQDNAAAANNVSKWTGTVYIFSLLGAFLSDSY GRFK
Sbjct: 61  QGLATLAFFGVGVNLVLFLTRVVGQDNAAAANNVSKWTGTVYIFSLLGAFLSDSYWGRFK 120

Query: 121 TCAIFQAIFVLGLASLSITSGLFLLKPKGCGDEHTPCDSHSSYHIALFYLSVYLVALGNG 180
           TCAIFQAIFV+GLASLSITS +FL++PKGCGDE TPC+S SS+H+ALFYLSVYLVALGNG
Sbjct: 121 TCAIFQAIFVIGLASLSITSYIFLVRPKGCGDEQTPCNSRSSFHMALFYLSVYLVALGNG 180

Query: 181 GYQPNIATFGADQFDEEDPKEGLSKIAFFSYFYLALNLGSLFSNTILGYFEDEGMWVIGF 240
           GYQPNIATFGADQFDEEDPKEGLSKIAFFSYFYLALNLGSLFSNTILGYFEDEGMWV+GF
Sbjct: 181 GYQPNIATFGADQFDEEDPKEGLSKIAFFSYFYLALNLGSLFSNTILGYFEDEGMWVLGF 240

Query: 241 WASTASAAMALILFLCGIPRYRHFTPKGNPLSRVSQVLVAATRKWKVQIMPNSEGLFDED 300
           WASTASAAMAL+LFLCGIPRYRHFTPKGNPLSRVSQV++AAT KWKVQIM +SEGLFDED
Sbjct: 241 WASTASAAMALLLFLCGIPRYRHFTPKGNPLSRVSQVVIAATTKWKVQIMTDSEGLFDED 300

Query: 301 EKDLVSNNGARKILHTNGFRFLDRAAAITSPEMDQFEDGTRNPWRLCTVTQVEEVKCVLR 360
           +K+LVS NGARKILHTNGF+FLDRAA ITS E DQ EDG RNPWRLCTVTQVEEVKC+LR
Sbjct: 301 DKELVS-NGARKILHTNGFKFLDRAAVITSSESDQLEDGARNPWRLCTVTQVEEVKCILR 360

Query: 361 LLPIWLCTILYSVVFTQMASLFVEQGAVMRTNISTFHIPPASMSSFDILSVAAFIFIYRR 420
           LLPIWLCTILYSVVFTQMASLFVEQGA MRTNISTFHIPPASMSSFDILSVAAFIFIYRR
Sbjct: 361 LLPIWLCTILYSVVFTQMASLFVEQGAAMRTNISTFHIPPASMSSFDILSVAAFIFIYRR 420

Query: 421 VIDPIYTRLTKSSLTELQRMGIGLVIAICAMVSAGTVEVFRLRYADKDCLHCEESSSLSI 480
           VIDP+Y RLTKSS+TELQRMGIGLVIAICAMVSAGTVE+FRL++A +DCLHC+ SSSLSI
Sbjct: 421 VIDPVYARLTKSSVTELQRMGIGLVIAICAMVSAGTVEIFRLKHATRDCLHCDGSSSLSI 480

Query: 481 FWQIPQYVLIGASEVFMYVGQLEFFNSQAPDGLKSFGSALCMTSISFGNYVSSLLVTIVM 540
           FWQIPQYVLIGASEVFMYVGQLEFFNSQAPDGLKSFGSALCMTSISFGNYVSSLLVTIVM
Sbjct: 481 FWQIPQYVLIGASEVFMYVGQLEFFNSQAPDGLKSFGSALCMTSISFGNYVSSLLVTIVM 540

Query: 541 KVSTNGNMPGWIPGNLNRGHLDRFFFLLAILTAADLVVYIICAKWYKYIKFESREAAAA 600
           K+S   NMPGWIPGNLNRGHLDRFFFLLA LTAADL VYI+CAKWYKYIKFESREA AA
Sbjct: 541 KISATDNMPGWIPGNLNRGHLDRFFFLLAALTAADLAVYILCAKWYKYIKFESREADAA 597

BLAST of Carg14875 vs. TAIR 10
Match: AT1G32450.1 (nitrate transporter 1.5 )

HSP 1 Score: 831.2 bits (2146), Expect = 5.2e-241
Identity = 417/600 (69.50%), Postives = 492/600 (82.00%), Query Frame = 0

Query: 1   MACLDISCLRQSTMQRKKTTMTTTAEKEVLTLDGAVDRHGRPAVRGKTGTWVAGILLLVN 60
           M+CL+I    + TM++K        E E  T DG VD +GRP++R  +G WVAGI++L+N
Sbjct: 1   MSCLEI--YNKDTMKKK--------EGEEETRDGTVDYYGRPSIRSNSGQWVAGIVILLN 60

Query: 61  QGLATLAFFGVGVNLVLFLTRVIGQDNAAAANNVSKWTGTVYIFSLLGAFLSDSYLGRFK 120
           QGLATLAFFGVGVNLVLFLTRV+ Q+NA AANNVSKWTGTVYIFSL+GAFLSDSY GR+K
Sbjct: 61  QGLATLAFFGVGVNLVLFLTRVLQQNNADAANNVSKWTGTVYIFSLVGAFLSDSYWGRYK 120

Query: 121 TCAIFQAIFVLGLASLSITSGLFLLKPKGCGDEHTPCDSHSSYHIALFYLSVYLVALGNG 180
           TCAIFQ IFV+GL+SLS++S +FL++P+GCGDE TPC SHS   I +FY S+YL+ALG G
Sbjct: 121 TCAIFQVIFVIGLSSLSLSSYMFLIRPRGCGDEVTPCGSHSMMEITMFYFSIYLIALGYG 180

Query: 181 GYQPNIATFGADQFDEEDPKEGLSKIAFFSYFYLALNLGSLFSNTILGYFEDEGMWVIGF 240
           GYQPNIAT GADQFDEE PKEG SKIAFFSYFYLALNLGSLFSNTILGYFEDEGMW +GF
Sbjct: 181 GYQPNIATLGADQFDEEHPKEGYSKIAFFSYFYLALNLGSLFSNTILGYFEDEGMWALGF 240

Query: 241 WASTASAAMALILFLCGIPRYRHFTPKGNPLSRVSQVLVAATRKWKVQI-MPNSEGLFDE 300
           WAST SA + LILFL G PRYR+F P GNPLSR  QVLVAAT+K  V+  +   E ++D 
Sbjct: 241 WASTGSAIIGLILFLVGTPRYRYFKPTGNPLSRFCQVLVAATKKSSVEAPLRGREEMYDG 300

Query: 301 D-EKDLVSNNGARKILHTNGFRFLDRAAAITSPEMDQFEDGTRNPWRLCTVTQVEEVKCV 360
           D E    S N  R+I+HT+ F+FLD+AA IT+ ++D  +  + NPWRLC VTQVEEVKC+
Sbjct: 301 DSEGKNASVNTGRRIVHTDEFKFLDKAAYITARDLDDKKQDSVNPWRLCPVTQVEEVKCI 360

Query: 361 LRLLPIWLCTILYSVVFTQMASLFVEQGAVMRTNISTFHIPPASMSSFDILSVAAFIFIY 420
           LRL+PIWLCTI+YSVVFTQMASLFVEQGA M T++S F IPPASMSSFDILSVA FIF+Y
Sbjct: 361 LRLMPIWLCTIIYSVVFTQMASLFVEQGAAMNTSVSDFKIPPASMSSFDILSVALFIFLY 420

Query: 421 RRVIDPIYTRLTKS---SLTELQRMGIGLVIAICAMVSAGTVEVFRLRYADKDCLHCEES 480
           RRV++P+  R  K+    +TEL RMGIGLVIA+ AM++AG VE +RL+YADK C HC+ S
Sbjct: 421 RRVLEPVANRFKKNGSKGITELHRMGIGLVIAVIAMIAAGIVECYRLKYADKSCTHCDGS 480

Query: 481 SSLSIFWQIPQYVLIGASEVFMYVGQLEFFNSQAPDGLKSFGSALCMTSISFGNYVSSLL 540
           SSLSIFWQ PQY LIGASEVFMYVGQLEFFN+Q PDGLKSFGSALCM S+S GN+VSSLL
Sbjct: 481 SSLSIFWQAPQYSLIGASEVFMYVGQLEFFNAQTPDGLKSFGSALCMMSMSMGNFVSSLL 540

Query: 541 VTIVMKVSTNGNMPGWIPGNLNRGHLDRFFFLLAILTAADLVVYIICAKWYKYIKFESRE 596
           VT+V+K+ST  +MPGWIP NLN+GHLDRF+FLLA LT+ DLVVYI CAKWYK I+ E ++
Sbjct: 541 VTMVVKISTEDHMPGWIPRNLNKGHLDRFYFLLAALTSIDLVVYIACAKWYKPIQLEGKD 590

BLAST of Carg14875 vs. TAIR 10
Match: AT4G21680.1 (NITRATE TRANSPORTER 1.8 )

HSP 1 Score: 800.0 bits (2065), Expect = 1.3e-231
Identity = 409/580 (70.52%), Postives = 467/580 (80.52%), Query Frame = 0

Query: 28  EVLTLDGAVDRHGRPAVRGKTGTWVAGILLLVNQGLATLAFFGVGVNLVLFLTRVIGQDN 87
           EV T DG+VDRHG PA+R  TG W+  IL+LVNQGLATLAFFGVGVNLVLFLTRV+GQDN
Sbjct: 9   EVCTQDGSVDRHGNPAIRANTGKWLTAILILVNQGLATLAFFGVGVNLVLFLTRVMGQDN 68

Query: 88  AAAANNVSKWTGTVYIFSLLGAFLSDSYLGRFKTCAIFQAIFVLGLASLSITSGLFLLKP 147
           A AANNVSKWTGTVYIFSLLGAFLSDSY GR+KTCAIFQA FV GL  LS+++G  LL+P
Sbjct: 69  AEAANNVSKWTGTVYIFSLLGAFLSDSYWGRYKTCAIFQASFVAGLMMLSLSTGALLLEP 128

Query: 148 KGCGDEHTPCDSHSSYHIALFYLSVYLVALGNGGYQPNIATFGADQFDEEDPKEGLSKIA 207
            GCG E +PC  HS++   LFYLSVYL+ALG GGYQPNIATFGADQFD ED  EG SKIA
Sbjct: 129 SGCGVEDSPCKPHSTFKTVLFYLSVYLIALGYGGYQPNIATFGADQFDAEDSVEGHSKIA 188

Query: 208 FFSYFYLALNLGSLFSNTILGYFEDEGMWVIGFWASTASAAMALILFLCGIPRYRHFTPK 267
           FFSYFYLALNLGSLFSNT+LGYFED+G W +GFWAS  SA   L+LFL G P+YRHFTP+
Sbjct: 189 FFSYFYLALNLGSLFSNTVLGYFEDQGEWPLGFWASAGSAFAGLVLFLIGTPKYRHFTPR 248

Query: 268 GNPLSRVSQVLVAATRKWKVQIMPNSEGLFDEDEKDLVSNNGARKILHTNGFRFLDRAAA 327
            +P SR  QVLVAATRK K+ +      L+D +        G +KILHT GFRFLDRAA 
Sbjct: 249 ESPWSRFCQVLVAATRKAKIDVHHEELNLYDSE----TQYTGDKKILHTKGFRFLDRAAI 308

Query: 328 IT-SPEMDQFEDGTR-NPWRLCTVTQVEEVKCVLRLLPIWLCTILYSVVFTQMASLFVEQ 387
           +T   E ++ E G++ +PWRLC+VTQVEEVKCVLRLLPIWLCTILYSVVFTQMASLFV Q
Sbjct: 309 VTPDDEAEKVESGSKYDPWRLCSVTQVEEVKCVLRLLPIWLCTILYSVVFTQMASLFVVQ 368

Query: 388 GAVMRTNISTFHIPPASMSSFDILSVAAFIFIYRRVIDPIYTRLTKS----SLTELQRMG 447
           GA M+TNI  F IP +SMSSFDILSVA FIF YRR +DP++ RL K+     LTELQRMG
Sbjct: 369 GAAMKTNIKNFRIPASSMSSFDILSVAFFIFAYRRFLDPLFARLNKTERNKGLTELQRMG 428

Query: 448 IGLVIAICAMVSAGTVEVFRLRYAD-KDCLHCEESSSLSIFWQIPQYVLIGASEVFMYVG 507
           IGLVIAI AM+SAG VE+ RL+  + +       SS+LSIFWQ+PQY+LIGASEVFMYVG
Sbjct: 429 IGLVIAIMAMISAGIVEIHRLKNKEPESATSISSSSTLSIFWQVPQYMLIGASEVFMYVG 488

Query: 508 QLEFFNSQAPDGLKSFGSALCMTSISFGNYVSSLLVTIVMKVSTNGNMPGWIPGNLNRGH 567
           QLEFFNSQAP GLKSF SALCM SIS GNYVSSLLV+IVMK+ST  ++ GWIP NLN+GH
Sbjct: 489 QLEFFNSQAPTGLKSFASALCMASISLGNYVSSLLVSIVMKISTTDDVHGWIPENLNKGH 548

Query: 568 LDRFFFLLAILTAADLVVYIICAKWYKYIKFESREAAAAT 601
           L+RF+FLLA LTAAD VVY+ICAKWYKYIK E+  + + T
Sbjct: 549 LERFYFLLAGLTAADFVVYLICAKWYKYIKSEASFSESVT 584

BLAST of Carg14875 vs. TAIR 10
Match: AT5G19640.1 (Major facilitator superfamily protein )

HSP 1 Score: 634.8 bits (1636), Expect = 7.1e-182
Identity = 339/548 (61.86%), Postives = 398/548 (72.63%), Query Frame = 0

Query: 47  KTGTWVAGILLLVNQGLATLAFFGVGVNLVLFLTRVIGQDNAAAANNVSKWTGTVYIFSL 106
           K G W   I+LLVNQGLATLAFFGVGVNLVLFLTRV+GQ NA AANNVSKWTGTVY+FSL
Sbjct: 59  KNGGWTNAIILLVNQGLATLAFFGVGVNLVLFLTRVMGQGNAEAANNVSKWTGTVYMFSL 118

Query: 107 LGAFLSDSYLGRFKTCAIFQAIFVLGLASLSITSGLFLLKPKGCGDEHTPCDSHSSYHIA 166
           +GAFLSDSY GR+ TC IFQ IFV+G+  LS  S  FL+KP+GCGD    C+  SS  +A
Sbjct: 119 VGAFLSDSYWGRYLTCTIFQVIFVIGVGLLSFVSWFFLIKPRGCGDGDLECNPPSSLGVA 178

Query: 167 LFYLSVYLVALGNGGYQPNIATFGADQFDEEDPKEGLSKIAFFSYFYLALNLGSLFSNTI 226
           +FYLSVYLVA G GG+QP +ATFGADQ D++      SK AFFSYFY ALN+G+LFSNTI
Sbjct: 179 IFYLSVYLVAFGYGGHQPTLATFGADQLDDDKN----SKAAFFSYFYFALNVGALFSNTI 238

Query: 227 LGYFEDEGMWVIGFWASTASAAMALILFLCGIPRYRHFTPKGNPLSRVSQVLVAATRKWK 286
           L YFED+G+W  GF  S  SA +AL+ FL    +YR+  P GNPL RV+QV VA  RKW 
Sbjct: 239 LVYFEDKGLWTEGFLVSLGSAIVALVAFLAPTRQYRYVKPCGNPLPRVAQVFVATARKWS 298

Query: 287 VQIMPNSEGLFDEDEKDLVSNNGARKILHTNGFRFLDRAAAITSPEMDQFEDGTR-NPWR 346
           V + P       E E    +  G+RKI H+  F FLDRAA IT  +     +GTR N WR
Sbjct: 299 V-VRPGDPHELYELEGPESAIKGSRKIFHSTKFLFLDRAAVITEND----RNGTRSNAWR 358

Query: 347 LCTVTQVEEVKCVLRLLPIWLCTILYSVVFTQMASLFVEQGAVMRTNISTFHIPPASMSS 406
           LC+VTQVEE KCV++LLPIWLCTI+YSV+FTQMASLFVEQG VM   +  FHIP ASMS 
Sbjct: 359 LCSVTQVEEAKCVMKLLPIWLCTIIYSVIFTQMASLFVEQGDVMNAYVGKFHIPAASMSV 418

Query: 407 FDILSVAAFIFIYRRVIDPIYTRLTKSSLTELQRMGIGLVIAICAMVSAGTVEVFRLRYA 466
           FDI SV     IYR +I P Y R      TEL RMGIGL+I I AMV+AG  E+ RL   
Sbjct: 419 FDIFSVFVSTGIYRHIIFP-YVR-----PTELMRMGIGLIIGIMAMVAAGLTEIQRL--- 478

Query: 467 DKDCLHCEESSSLSIFWQIPQYVLIGASEVFMYVGQLEFFNSQAPDGLKSFGSALCMTSI 526
            K  +  ++ S L+I WQIPQYVL+GASEVFMYVGQLEFFN QAPDGLK+ GS+LCM S+
Sbjct: 479 -KRVVPGQKESELTILWQIPQYVLVGASEVFMYVGQLEFFNGQAPDGLKNLGSSLCMASM 538

Query: 527 SFGNYVSSLLVTIVMKVSTNG-NMPGWIPGNLNRGHLDRFFFLLAILTAADLVVYIICAK 586
           + GNYVSSL+V IVM ++  G N PGWIP NLN GH+DRF+FL+A L A D VVY+I AK
Sbjct: 539 ALGNYVSSLMVNIVMAITKRGENSPGWIPENLNEGHMDRFYFLIAALAAIDFVVYLIFAK 587

Query: 587 WYKYIKFE 593
           WY+ I  +
Sbjct: 599 WYQPISHD 587

BLAST of Carg14875 vs. TAIR 10
Match: AT3G54140.1 (peptide transporter 1 )

HSP 1 Score: 490.7 bits (1262), Expect = 1.7e-138
Identity = 256/572 (44.76%), Postives = 379/572 (66.26%), Query Frame = 0

Query: 26  EKEVLTLDGAVDRHGRPAVRGKTGTWVAGILLLVNQGLATLAFFGVGVNLVLFLTRVIGQ 85
           EK+V T DG VD H  PA + KTG W A   +L N+    LA++G+G NLV +L   + Q
Sbjct: 3   EKDVYTQDGTVDIHKNPANKEKTGNWKACRFILGNECCERLAYYGMGTNLVNYLESRLNQ 62

Query: 86  DNAAAANNVSKWTGTVYIFSLLGAFLSDSYLGRFKTCAIFQAIFVLGLASLSITSGLFLL 145
            NA AANNV+ W+GT YI  L+GAF++D+YLGR+ T A F  I+V G+  L++++ +  L
Sbjct: 63  GNATAANNVTNWSGTCYITPLIGAFIADAYLGRYWTIATFVFIYVSGMTLLTLSASVPGL 122

Query: 146 KPKGCGDEHTPCDSHSSYHIALFYLSVYLVALGNGGYQPNIATFGADQFDEEDPKEGLSK 205
           KP  C  +   C  +SS   A+F++++Y++ALG GG +P +++FGADQFDE D  E + K
Sbjct: 123 KPGNCNAD--TCHPNSS-QTAVFFVALYMIALGTGGIKPCVSSFGADQFDENDENEKIKK 182

Query: 206 IAFFSYFYLALNLGSLFSNTILGYFEDEGMWVIGFWASTASAAMALILFLCGIPRYRHFT 265
            +FF++FY ++N+G+L + T+L + +    W  GF   T +  +A+  F  G   YR   
Sbjct: 183 SSFFNWFYFSINVGALIAATVLVWIQMNVGWGWGFGVPTVAMVIAVCFFFFGSRFYRLQR 242

Query: 266 PKGNPLSRVSQVLVAATRKWKVQIMPNSEGLFDEDEKDLVSNNGARKILHTNGFRFLDRA 325
           P G+PL+R+ QV+VAA RK  V++ P  + L  E   D  +  G+RK++HT+  +F D+A
Sbjct: 243 PGGSPLTRIFQVIVAAFRKISVKV-PEDKSLLFETADDESNIKGSRKLVHTDNLKFFDKA 302

Query: 326 AAITSPEMDQFEDGTRNPWRLCTVTQVEEVKCVLRLLPIWLCTILYSVVFTQMASLFVEQ 385
           A     + D  +DG  NPWRLC+VTQVEE+K ++ LLP+W   I+++ V++QM+++FV Q
Sbjct: 303 A--VESQSDSIKDGEVNPWRLCSVTQVEELKSIITLLPVWATGIVFATVYSQMSTMFVLQ 362

Query: 386 GAVMRTNI-STFHIPPASMSSFDILSVAAFIFIYRRVIDPIYTRLTKS--SLTELQRMGI 445
           G  M  ++   F IP AS+S FD +SV  +  +Y + I P+  + T++    T+LQRMGI
Sbjct: 363 GNTMDQHMGKNFEIPSASLSLFDTVSVLFWTPVYDQFIIPLARKFTRNERGFTQLQRMGI 422

Query: 446 GLVIAICAMVSAGTVEVFRLRYADKDCLHCEESSSLSIFWQIPQYVLIGASEVFMYVGQL 505
           GLV++I AM++AG +EV RL Y      + ++   +SIFWQIPQY+LIG +EVF ++GQL
Sbjct: 423 GLVVSIFAMITAGVLEVVRLDYVKTHNAYDQKQIHMSIFWQIPQYLLIGCAEVFTFIGQL 482

Query: 506 EFFNSQAPDGLKSFGSALCMTSISFGNYVSSLLVTIVMKVSTNGNMPGWIPGNLNRGHLD 565
           EFF  QAPD ++S  SAL +T+++ GNY+S++LVT+VMK++     PGWIP NLNRGHLD
Sbjct: 483 EFFYDQAPDAMRSLCSALSLTTVALGNYLSTVLVTVVMKITKKNGKPGWIPDNLNRGHLD 542

Query: 566 RFFFLLAILTAADLVVYIICAKWYKYIKFESR 595
            FF+LLA L+  + +VY+  +K YKY K   R
Sbjct: 543 YFFYLLATLSFLNFLVYLWISKRYKYKKAVGR 568

BLAST of Carg14875 vs. TAIR 10
Match: AT5G01180.1 (peptide transporter 5 )

HSP 1 Score: 459.1 bits (1180), Expect = 5.3e-129
Identity = 246/568 (43.31%), Postives = 372/568 (65.49%), Query Frame = 0

Query: 26  EKEVLTLDGAVDRHGRPAVRGKTGTWVAGILLLVNQGLATLAFFGVGVNLVLFLTRVIGQ 85
           +K++ T DG +D H +PA + KTGTW A   +L  +    LA++G+  NL+ +L + +  
Sbjct: 4   DKDIYTKDGTLDIHKKPANKNKTGTWKACRFILGTECCERLAYYGMSTNLINYLEKQMNM 63

Query: 86  DNAAAANNVSKWTGTVYIFSLLGAFLSDSYLGRFKTCAIFQAIFVLGLASLSITSGLFLL 145
           +N +A+ +VS W+GT Y   L+GAF++D+YLGR+ T A F  I++ G+  L+I++ +  L
Sbjct: 64  ENVSASKSVSNWSGTCYATPLIGAFIADAYLGRYWTIASFVVIYIAGMTLLTISASVPGL 123

Query: 146 KPKGCGDEHTPCDSHSSYHIALFYLSVYLVALGNGGYQPNIATFGADQFDEEDPKEGLSK 205
            P   G+    C + ++   A+ ++++YL+ALG GG +P +++FGADQFD+ D KE  SK
Sbjct: 124 TPTCSGE---TCHA-TAGQTAITFIALYLIALGTGGIKPCVSSFGADQFDDTDEKEKESK 183

Query: 206 IAFFSYFYLALNLGSLFSNTILGYFEDEGMWVIGFWASTASAAMALILFLCGIPRYRHFT 265
            +FF++FY  +N+G++ ++++L + +    W  G    T + A+A++ F  G   YR   
Sbjct: 184 SSFFNWFYFVINVGAMIASSVLVWIQMNVGWGWGLGVPTVAMAIAVVFFFAGSNFYRLQK 243

Query: 266 PKGNPLSRVSQVLVAATRKWKVQIMPNSEGLFDEDEKDLVSNNGARKILHTNGFRFLDRA 325
           P G+PL+R+ QV+VA+ RK KV+I P  E L  E++    S  G+RK+ HT    F D+A
Sbjct: 244 PGGSPLTRMLQVIVASCRKSKVKI-PEDESLLYENQDAESSIIGSRKLEHTKILTFFDKA 303

Query: 326 AAITSPEMDQFEDGTRNPWRLCTVTQVEEVKCVLRLLPIWLCTILYSVVFTQMASLFVEQ 385
           A  T  E D       + W+LCTVTQVEE+K ++RLLPIW   I+++ V++QM ++FV Q
Sbjct: 304 AVET--ESDNKGAAKSSSWKLCTVTQVEELKALIRLLPIWATGIVFASVYSQMGTVFVLQ 363

Query: 386 GAVMRTNIS-TFHIPPASMSSFDILSVAAFIFIYRRVIDPIYTRLT--KSSLTELQRMGI 445
           G  +  ++   F IP AS+S FD LSV  +  +Y ++I P   + T  +   T+LQR+GI
Sbjct: 364 GNTLDQHMGPNFKIPSASLSLFDTLSVLFWAPVYDKLIVPFARKYTGHERGFTQLQRIGI 423

Query: 446 GLVIAICAMVSAGTVEVFRLRYADKDCLHCEESSSLSIFWQIPQYVLIGASEVFMYVGQL 505
           GLVI+I +MVSAG +EV RL Y     L+ EE+  ++IFWQ+PQY L+G +EVF ++GQL
Sbjct: 424 GLVISIFSMVSAGILEVARLNYVQTHNLYNEETIPMTIFWQVPQYFLVGCAEVFTFIGQL 483

Query: 506 EFFNSQAPDGLKSFGSALCMTSISFGNYVSSLLVTIVMKVSTNGNMPGWIPGNLNRGHLD 565
           EFF  QAPD ++S  SAL +T+I+FGNY+S+ LVT+V KV+ +G  PGWI  NLN GHLD
Sbjct: 484 EFFYDQAPDAMRSLCSALSLTAIAFGNYLSTFLVTLVTKVTRSGGRPGWIAKNLNNGHLD 543

Query: 566 RFFFLLAILTAADLVVYIICAKWYKYIK 591
            FF+LLA L+  + +VY+  AKWY Y K
Sbjct: 544 YFFWLLAGLSFLNFLVYLWIAKWYTYKK 564

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
KAG7018911.10.0e+00100.00Protein NRT1/ PTR FAMILY 7.3 [Cucurbita argyrosperma subsp. argyrosperma][more]
KAG6582526.10.0e+0099.34Protein NRT1/ PTR FAMILY 7.3, partial [Cucurbita argyrosperma subsp. sororia][more]
XP_023526114.10.0e+0098.51protein NRT1/ PTR FAMILY 7.3-like [Cucurbita pepo subsp. pepo][more]
XP_022924814.10.0e+0099.17protein NRT1/ PTR FAMILY 7.3-like isoform X1 [Cucurbita moschata][more]
XP_022980257.10.0e+0098.01protein NRT1/ PTR FAMILY 7.3-like [Cucurbita maxima][more]
Match NameE-valueIdentityDescription
Q9LQL27.3e-24069.50Protein NRT1/ PTR FAMILY 7.3 OS=Arabidopsis thaliana OX=3702 GN=NPF7.3 PE=1 SV=2[more]
Q8GXN21.8e-23070.52Protein NRT1/ PTR FAMILY 7.2 OS=Arabidopsis thaliana OX=3702 GN=NPF7.2 PE=2 SV=2[more]
Q3E9B51.0e-18061.86Protein NRT1/ PTR FAMILY 7.1 OS=Arabidopsis thaliana OX=3702 GN=NPF7.1 PE=2 SV=1[more]
Q9M3902.3e-13744.76Protein NRT1/ PTR FAMILY 8.1 OS=Arabidopsis thaliana OX=3702 GN=NPF8.1 PE=1 SV=1[more]
Q9LFB87.5e-12843.31Protein NRT1/ PTR FAMILY 8.2 OS=Arabidopsis thaliana OX=3702 GN=NPF8.2 PE=2 SV=1[more]
Match NameE-valueIdentityDescription
A0A6J1EA960.0e+0099.17protein NRT1/ PTR FAMILY 7.3-like isoform X1 OS=Cucurbita moschata OX=3662 GN=LO... [more]
A0A6J1IQW80.0e+0098.01protein NRT1/ PTR FAMILY 7.3-like OS=Cucurbita maxima OX=3661 GN=LOC111479679 PE... [more]
A0A6J1EG490.0e+0098.51protein NRT1/ PTR FAMILY 7.3-like isoform X2 OS=Cucurbita moschata OX=3662 GN=LO... [more]
A0A0A0L6J78.4e-30887.85Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_3G127170 PE=3 SV=1[more]
A0A6J1CZL91.1e-30788.65protein NRT1/ PTR FAMILY 7.3-like OS=Momordica charantia OX=3673 GN=LOC111016031... [more]
Match NameE-valueIdentityDescription
AT1G32450.15.2e-24169.50nitrate transporter 1.5 [more]
AT4G21680.11.3e-23170.52NITRATE TRANSPORTER 1.8 [more]
AT5G19640.17.1e-18261.86Major facilitator superfamily protein [more]
AT3G54140.11.7e-13844.76peptide transporter 1 [more]
AT5G01180.15.3e-12943.31peptide transporter 5 [more]
InterPro
Analysis Name: InterPro Annotations of Silver-seed gourd (SMH-JMG-627) v2
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR000109Proton-dependent oligopeptide transporter familyPFAMPF00854PTR2coord: 119..547
e-value: 7.1E-95
score: 318.1
IPR000109Proton-dependent oligopeptide transporter familyPANTHERPTHR11654OLIGOPEPTIDE TRANSPORTER-RELATEDcoord: 28..596
IPR036259MFS transporter superfamilyGENE3D1.20.1250.20MFS general substrate transporter like domainscoord: 22..595
e-value: 6.2E-178
score: 594.2
IPR036259MFS transporter superfamilySUPERFAMILY103473MFS general substrate transportercoord: 42..592
NoneNo IPR availablePANTHERPTHR11654:SF90PROTEIN NRT1/ PTR FAMILY 7.3coord: 28..596
NoneNo IPR availableCDDcd17419MFS_NPF7coord: 51..585
e-value: 0.0
score: 689.882

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Carg14875-RACarg14875-RAmRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0006817 phosphate ion transport
biological_process GO:0050896 response to stimulus
biological_process GO:0055085 transmembrane transport
cellular_component GO:0016021 integral component of membrane
cellular_component GO:0016020 membrane
molecular_function GO:0022857 transmembrane transporter activity