Carg14852 (gene) Silver-seed gourd (SMH-JMG-627) v2

Overview


Name	Carg14852
Type	gene
Organism	Cucurbita argyrosperma subsp. argyrosperma cv. SMH-JMG-627 (Silver-seed gourd (SMH-JMG-627) v2)
Description	tRNA (Guanine-N(7)-)-methyltransferase
Location	Carg_Chr14: 13882517 .. 13885793 (-)
RNA-Seq Expression	Carg14852
Synteny	Carg14852

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRpolypeptideCDSthree_prime_UTR

Hold the cursor over a type above to highlight its positions in the sequence below.

CGGCAGCGCCATTCTTGAGAGCCCAACGGACGCGGCAAAATGGCTTCGTCCCTTTGCTTCCAGTTACACACTTGCTCCCAGCTTCGTTGCATTGCTGCTGCGAAGGCGGCAGCGGCAGCGGCGCCGTCGAGCGTCGCAACAGCAAACCGAACGTTATCTCTGTTAGTAAAACGACCATTTCTTCAATCCTTCTGTTTACCATCGGGCTCTTCCGCCGAGCTTTGCGAGCGGCAACAACTCCGGAGTCCGGAACTTGTGGCGTTGGAATATGCGGACCTTAATCTCTCTCACAAGATCTTCGAGGTTTGTTTTCTGTTCGTGAATTTTGCCCTTGATTTCTGAAATCGTTGGCGAGTAATTGAAAATTGAATGTGGTGACTAGAATTGATGTGCATTTGACTTGGTTTTGGGAATTGCAGGATTCAGGTCACGTCAGAATCAGGCAGCATGTTAATCCACTTAGCTCTTCTTTCTCTGTATGTAATTTGAGTTTTGAAAGTGGATTTGGTTTCTCTCTGATGAGAAATTTTAGGAGCTGTGGCTTTGAAATTTTAGCTTAGTGTTGCTTAATTGAAGGAAAAGCTCTATCTCTTTTACCTTTCAGGTCCCTACACCAGCACCTAACTGGGATGAAGTCTTCAAGGACCCCACGTTGCCCCTCATGGTCGATATAGGAAGTGGTGAGTGAGCTGATGCATTCCCTGTTATATACCTCTTACATATCTGTTTTCTTCATAATAGCTACTTTCTTAGTACATACTTCGCTTTCACGAGATAATATTTGATTCTGTGTAGCATTTGGCTGAATCCATATTCCTAAAATCGTTTTTAACTTGCTTTTGATTTCTAAGTCAATCCTCAGTCGTTGAAAATCAGTTTTGATGACATGAAAATTGTAACAGCCCAATCCCACCCCCACCGATAGTAGATACTGTCCTCTTTGGGCTTTCCCTTTCGGGCTTCCCCTCAAGGTTTTTAAAATGCGTCGGGTAATGAGAAGTTTCCACACCCTTATAAAAAATGTTTCGTTCTCCTCCCCAACCGATGTGGGACCTCACAATCCATCCCTTTTCGGGGCACAGCATCCTTGCCGCACACCACCTTGTGTCCACCCCCTTCGGGGTCTAACATCCTTGCTGGTACACCACCTTGTGTCCACCCCCCTTCGGGGCTCAGTCTCCTCGTTGGCACATCGCCTGATGTCTGGCTCTGATACCATTTGTAACAACCCAAGTCCACCGCTAGCAAATATTATCCTCTTTGAGCTTTTTCCCTTTCGGGCTTTCCCTCAATATTCTTAAAATGCGTATGCTAGGGAGAGGTTTCCACGCCCTTATAAAGAATAATTCGTTCTCCTCCCTAACCGACGTGGGATCTTACAAAAATAATGTTTAAAAGTGTATTATTTCATTTTGAATGGTTAAACTCATGTTTAGGAGTGATTTTAACCATCACTGATATTGCTTATATTATAATTAGAATCTTACCCATAATCCTCCTGCTTTGGCCAAATAGAGGATAGCCATGAACAAGTGTTTGATGCTACTGCTTGATTTTGAAAACAAGTTCAAAACATAATTTAGAGATCCTACCATTTGCTCAATCTGTTTGGTGATGTTCATTGAACCAGCATGAAGAAGGAAATATTATCCTAAAGCATCATATTGATTACTGCATTCAAAGATCATCTTTACCGTGCACTGTAAATCAAAATTTATCTCGATATGCGTTATAAAACAGCTCTAATCATGTAATCAATACGTTACATGTCGAGCAAAATGCTCCAAGCAATAGCAGCAACGAGCTCGCTAGTAAACATTAAGTTTAATACAATGTAGTCGCCTAAGTCGCTCTGGAGAATTTACACAGGAAAGAATTCTGTTGGTAATGATATTAAGGTTTCAAATGCTAATTGCCTATAGTATCGTATTGTATTCACATGCAAGAAAATAATTTATCAGATTAAGACATGCATGCTGTAATTGTTAGTCGTGAGTCCCGCGTTGGCTAATTTAGGGAATGATCATGGGTTTATAAGTAAAGGAATACATTTCCATTGGTATGAGGCGTTTTGGGAAAGCCCAAAGCAAAGCCATGAGAGCTTATGCTCAAAGTGGATAATATCATACCATTGTTGGGAGTCGTGATTCCTAACAATAATGATTTACGTAACATCTTTACTTTCATGCATTTTTAAGAATTCATGAGACAATTTAATGCCTTTGATTTCCAATCAAGAATCTAACAGAACAAGTATTCCTATTCCCAGGTAGTGGTAGATTTCTCTTGTGGCTTGCAAAAAAACATCTTGATTCAAGAAATTTCTTGGGTCTCGAAATACGACAGAAAGTACGGGACCGAAAATTTTCACTTTTGATGCACCCTTAGTTGGTCTGAACTATTTTATCCTCGTTCTATCGAGGCATTATATCGTGCTTTGATTCCTTACGTGCTCTTGCAGTTGGTCAAGCGTGCCGATGTCTGGGTCAAAGAGTTGGGATTTAACAATGTGTAAGGACCTTAACTTCATAGCAGGTAGTGAAAAACAAGATCACCTTTTTGCTATTGGTACATTAGTAATGAACTTCTTCTTGAACTTGAATGCAGACATTTCATGTTTGCAAATGCTACAATTTCTTTTAAACAGATAATATCTTCTTATCCTGGACCCCTGATGCTGGTTTCCATATTGGTTAGTGCAACCCCCCCTCCCCGAACGATTCAACCCGAACTTTCTCATTGAATATCAACGAAGATAGAACTCACTCGTGCTTAATCTTGTCACAGTGCCCAGATCCCCATTTCAAGAAACGACATCATAAGCGACGGGTTTTGCAGAAGCCATTAGTAGATTCTATAATAGACAGTTTAATGCCAGGAGGGCAGGTATATGATCTGCCAATCCATGACATTCAATGGTTTTTTTTGCCTGTTTGATGAGTCCGATGCTGGAAATTTTGTTGTATATGATTCAACAGGTGTTCATACAATCCGACGTGCTCGAAGTGGCAGTTGACATGAGACTTTGGTTCGATTCTGTTCCCGATGTTTTGAAACACGTCGACGTGCTCGACCAGAGTCTTTTGTGTGATAAAGAGGGATGGTTATTGAAGAATCCAATGGGAATAAGGACTGAAAGAGAAGTTCACGCTGAATCCGAAGGTGCGAACATTTACAGAAGAATGTATCAGAAGTATGTTTGCATTTGAGTCATCATTCACTGTTTTTTTTTTTTTTTGTTCTTTTCTAGAAATATATTTATTGCATCTTTATAATT

mRNA sequence

CGGCAGCGCCATTCTTGAGAGCCCAACGGACGCGGCAAAATGGCTTCGTCCCTTTGCTTCCAGTTACACACTTGCTCCCAGCTTCGTTGCATTGCTGCTGCGAAGGCGGCAGCGGCAGCGGCGCCGTCGAGCGTCGCAACAGCAAACCGAACGTTATCTCTGTTAGTAAAACGACCATTTCTTCAATCCTTCTGTTTACCATCGGGCTCTTCCGCCGAGCTTTGCGAGCGGCAACAACTCCGGAGTCCGGAACTTGTGGCGTTGGAATATGCGGACCTTAATCTCTCTCACAAGATCTTCGAGGATTCAGGTCACGTCAGAATCAGGCAGCATGTTAATCCACTTAGCTCTTCTTTCTCTGTCCCTACACCAGCACCTAACTGGGATGAAGTCTTCAAGGACCCCACGTTGCCCCTCATGGTCGATATAGGAAGTGGTAGTGGTAGATTTCTCTTGTGGCTTGCAAAAAAACATCTTGATTCAAGAAATTTCTTGGGTCTCGAAATACGACAGAAATTGGTCAAGCGTGCCGATGTCTGGGTCAAAGAGTTGGGATTTAACAATGTACATTTCATGTTTGCAAATGCTACAATTTCTTTTAAACAGATAATATCTTCTTATCCTGGACCCCTGATGCTGGTTTCCATATTGTGCCCAGATCCCCATTTCAAGAAACGACATCATAAGCGACGGGTTTTGCAGAAGCCATTAGTAGATTCTATAATAGACAGTTTAATGCCAGGAGGGCAGGTGTTCATACAATCCGACGTGCTCGAAGTGGCAGTTGACATGAGACTTTGGTTCGATTCTGTTCCCGATGTTTTGAAACACGTCGACGTGCTCGACCAGAGTCTTTTGTGTGATAAAGAGGGATGGTTATTGAAGAATCCAATGGGAATAAGGACTGAAAGAGAAGTTCACGCTGAATCCGAAGGTGCGAACATTTACAGAAGAATGTATCAGAAGTATGTTTGCATTTGAGTCATCATTCACTGTTTTTTTTTTTTTTTGTTCTTTTCTAGAAATATATTTATTGCATCTTTATAATT

Coding sequence (CDS)

ATGGCTTCGTCCCTTTGCTTCCAGTTACACACTTGCTCCCAGCTTCGTTGCATTGCTGCTGCGAAGGCGGCAGCGGCAGCGGCGCCGTCGAGCGTCGCAACAGCAAACCGAACGTTATCTCTGTTAGTAAAACGACCATTTCTTCAATCCTTCTGTTTACCATCGGGCTCTTCCGCCGAGCTTTGCGAGCGGCAACAACTCCGGAGTCCGGAACTTGTGGCGTTGGAATATGCGGACCTTAATCTCTCTCACAAGATCTTCGAGGATTCAGGTCACGTCAGAATCAGGCAGCATGTTAATCCACTTAGCTCTTCTTTCTCTGTCCCTACACCAGCACCTAACTGGGATGAAGTCTTCAAGGACCCCACGTTGCCCCTCATGGTCGATATAGGAAGTGGTAGTGGTAGATTTCTCTTGTGGCTTGCAAAAAAACATCTTGATTCAAGAAATTTCTTGGGTCTCGAAATACGACAGAAATTGGTCAAGCGTGCCGATGTCTGGGTCAAAGAGTTGGGATTTAACAATGTACATTTCATGTTTGCAAATGCTACAATTTCTTTTAAACAGATAATATCTTCTTATCCTGGACCCCTGATGCTGGTTTCCATATTGTGCCCAGATCCCCATTTCAAGAAACGACATCATAAGCGACGGGTTTTGCAGAAGCCATTAGTAGATTCTATAATAGACAGTTTAATGCCAGGAGGGCAGGTGTTCATACAATCCGACGTGCTCGAAGTGGCAGTTGACATGAGACTTTGGTTCGATTCTGTTCCCGATGTTTTGAAACACGTCGACGTGCTCGACCAGAGTCTTTTGTGTGATAAAGAGGGATGGTTATTGAAGAATCCAATGGGAATAAGGACTGAAAGAGAAGTTCACGCTGAATCCGAAGGTGCGAACATTTACAGAAGAATGTATCAGAAGTATGTTTGCATTTGA

Protein sequence

MASSLCFQLHTCSQLRCIAAAKAAAAAAPSSVATANRTLSLLVKRPFLQSFCLPSGSSAELCERQQLRSPELVALEYADLNLSHKIFEDSGHVRIRQHVNPLSSSFSVPTPAPNWDEVFKDPTLPLMVDIGSGSGRFLLWLAKKHLDSRNFLGLEIRQKLVKRADVWVKELGFNNVHFMFANATISFKQIISSYPGPLMLVSILCPDPHFKKRHHKRRVLQKPLVDSIIDSLMPGGQVFIQSDVLEVAVDMRLWFDSVPDVLKHVDVLDQSLLCDKEGWLLKNPMGIRTEREVHAESEGANIYRRMYQKYVCI

Homology

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BLAST of Carg14852 vs. NCBI nr
Match: KAG7018929.1 (trmB [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 629.8 bits (1623), Expect = 1.3e-176
Identity = 313/313 (100.00%), Postives = 313/313 (100.00%), Query Frame = 0


Query: 1   MASSLCFQLHTCSQLRCIAAAKAAAAAAPSSVATANRTLSLLVKRPFLQSFCLPSGSSAE 60
           MASSLCFQLHTCSQLRCIAAAKAAAAAAPSSVATANRTLSLLVKRPFLQSFCLPSGSSAE
Sbjct: 1   MASSLCFQLHTCSQLRCIAAAKAAAAAAPSSVATANRTLSLLVKRPFLQSFCLPSGSSAE 60

Query: 61  LCERQQLRSPELVALEYADLNLSHKIFEDSGHVRIRQHVNPLSSSFSVPTPAPNWDEVFK 120
           LCERQQLRSPELVALEYADLNLSHKIFEDSGHVRIRQHVNPLSSSFSVPTPAPNWDEVFK
Sbjct: 61  LCERQQLRSPELVALEYADLNLSHKIFEDSGHVRIRQHVNPLSSSFSVPTPAPNWDEVFK 120

Query: 121 DPTLPLMVDIGSGSGRFLLWLAKKHLDSRNFLGLEIRQKLVKRADVWVKELGFNNVHFMF 180
           DPTLPLMVDIGSGSGRFLLWLAKKHLDSRNFLGLEIRQKLVKRADVWVKELGFNNVHFMF
Sbjct: 121 DPTLPLMVDIGSGSGRFLLWLAKKHLDSRNFLGLEIRQKLVKRADVWVKELGFNNVHFMF 180

Query: 181 ANATISFKQIISSYPGPLMLVSILCPDPHFKKRHHKRRVLQKPLVDSIIDSLMPGGQVFI 240
           ANATISFKQIISSYPGPLMLVSILCPDPHFKKRHHKRRVLQKPLVDSIIDSLMPGGQVFI
Sbjct: 181 ANATISFKQIISSYPGPLMLVSILCPDPHFKKRHHKRRVLQKPLVDSIIDSLMPGGQVFI 240

Query: 241 QSDVLEVAVDMRLWFDSVPDVLKHVDVLDQSLLCDKEGWLLKNPMGIRTEREVHAESEGA 300
           QSDVLEVAVDMRLWFDSVPDVLKHVDVLDQSLLCDKEGWLLKNPMGIRTEREVHAESEGA
Sbjct: 241 QSDVLEVAVDMRLWFDSVPDVLKHVDVLDQSLLCDKEGWLLKNPMGIRTEREVHAESEGA 300

Query: 301 NIYRRMYQKYVCI 314
           NIYRRMYQKYVCI
Sbjct: 301 NIYRRMYQKYVCI 313

[Close]

BLAST of Carg14852 vs. NCBI nr
Match: XP_023527130.1 (uncharacterized protein LOC111790461 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 625.5 bits (1612), Expect = 2.4e-175
Identity = 309/313 (98.72%), Postives = 312/313 (99.68%), Query Frame = 0


Query: 1   MASSLCFQLHTCSQLRCIAAAKAAAAAAPSSVATANRTLSLLVKRPFLQSFCLPSGSSAE 60
           MASSLCFQLHTCSQLRCIAAAKAAA AAPSSVATANRTLSLL+KRPFLQSFCLPSGSSAE
Sbjct: 1   MASSLCFQLHTCSQLRCIAAAKAAAVAAPSSVATANRTLSLLLKRPFLQSFCLPSGSSAE 60

Query: 61  LCERQQLRSPELVALEYADLNLSHKIFEDSGHVRIRQHVNPLSSSFSVPTPAPNWDEVFK 120
           LCERQQLRSPELVALEYADLNLSHKIFEDSGHVRIRQHVNPLSSSFSVPTPAPNWDEVFK
Sbjct: 61  LCERQQLRSPELVALEYADLNLSHKIFEDSGHVRIRQHVNPLSSSFSVPTPAPNWDEVFK 120

Query: 121 DPTLPLMVDIGSGSGRFLLWLAKKHLDSRNFLGLEIRQKLVKRADVWVKELGFNNVHFMF 180
           DPTLPLMVDIGSGSGRFLLWLAKKHLDSRNFLGLEIRQKLVKRADVWVKELGFNNVHFMF
Sbjct: 121 DPTLPLMVDIGSGSGRFLLWLAKKHLDSRNFLGLEIRQKLVKRADVWVKELGFNNVHFMF 180

Query: 181 ANATISFKQIISSYPGPLMLVSILCPDPHFKKRHHKRRVLQKPLVDSIIDSLMPGGQVFI 240
           ANATISFKQIISSYPGPLMLVSILCPDPHFKKRHHKRRVLQKPLVDSIIDSLMPGGQVFI
Sbjct: 181 ANATISFKQIISSYPGPLMLVSILCPDPHFKKRHHKRRVLQKPLVDSIIDSLMPGGQVFI 240

Query: 241 QSDVLEVAVDMRLWFDSVPDVLKHVDVLDQSLLCDKEGWLLKNPMGIRTEREVHAESEGA 300
           QSDVLEVAVDMRLWFDSVPDVLKHVDVLDQSLLCDK+GWLLKNPMGIRTERE+HAESEGA
Sbjct: 241 QSDVLEVAVDMRLWFDSVPDVLKHVDVLDQSLLCDKDGWLLKNPMGIRTEREIHAESEGA 300

Query: 301 NIYRRMYQKYVCI 314
           NIYRRMYQKYVCI
Sbjct: 301 NIYRRMYQKYVCI 313

[Close]

BLAST of Carg14852 vs. NCBI nr
Match: XP_022924717.1 (uncharacterized protein LOC111432133 [Cucurbita moschata])

HSP 1 Score: 623.6 bits (1607), Expect = 9.1e-175
Identity = 309/313 (98.72%), Postives = 312/313 (99.68%), Query Frame = 0


Query: 1   MASSLCFQLHTCSQLRCIAAAKAAAAAAPSSVATANRTLSLLVKRPFLQSFCLPSGSSAE 60
           MASSLCFQLHTCSQLRCIAAAKAAAAAAPSSVATANRTLSLLVKRPFLQSFCLPSGSSAE
Sbjct: 1   MASSLCFQLHTCSQLRCIAAAKAAAAAAPSSVATANRTLSLLVKRPFLQSFCLPSGSSAE 60

Query: 61  LCERQQLRSPELVALEYADLNLSHKIFEDSGHVRIRQHVNPLSSSFSVPTPAPNWDEVFK 120
           LCERQQLRSPELVALEYADLNLSHKIFEDSGHVRIRQHVNPLSSSFSVPTPAPNWDEVFK
Sbjct: 61  LCERQQLRSPELVALEYADLNLSHKIFEDSGHVRIRQHVNPLSSSFSVPTPAPNWDEVFK 120

Query: 121 DPTLPLMVDIGSGSGRFLLWLAKKHLDSRNFLGLEIRQKLVKRADVWVKELGFNNVHFMF 180
           DPTLPLMVDIGSGSGRFLLWLAKKHLDSRNFLGLEIRQKLVKRADVWVKELGFNNVHFMF
Sbjct: 121 DPTLPLMVDIGSGSGRFLLWLAKKHLDSRNFLGLEIRQKLVKRADVWVKELGFNNVHFMF 180

Query: 181 ANATISFKQIISSYPGPLMLVSILCPDPHFKKRHHKRRVLQKPLVDSIIDSLMPGGQVFI 240
           ANATISFKQIISSYPGPLMLVSILCPDPHFKKRHHKRRVLQKPLVDSIIDSLMPGGQVFI
Sbjct: 181 ANATISFKQIISSYPGPLMLVSILCPDPHFKKRHHKRRVLQKPLVDSIIDSLMPGGQVFI 240

Query: 241 QSDVLEVAVDMRLWFDSVPDVLKHVDVLDQSLLCDKEGWLLKNPMGIRTEREVHAESEGA 300
           QSDVLEVAVDMRLWFDSVP+VLKHVDV DQSLLCDK+GWLLKNPMGIRTERE+HAESEGA
Sbjct: 241 QSDVLEVAVDMRLWFDSVPNVLKHVDVHDQSLLCDKDGWLLKNPMGIRTEREIHAESEGA 300

Query: 301 NIYRRMYQKYVCI 314
           NIYRRMYQKYVCI
Sbjct: 301 NIYRRMYQKYVCI 313

[Close]

BLAST of Carg14852 vs. NCBI nr
Match: XP_022980361.1 (uncharacterized protein LOC111479752 [Cucurbita maxima])

HSP 1 Score: 620.2 bits (1598), Expect = 1.0e-173
Identity = 307/313 (98.08%), Postives = 310/313 (99.04%), Query Frame = 0


Query: 1   MASSLCFQLHTCSQLRCIAAAKAAAAAAPSSVATANRTLSLLVKRPFLQSFCLPSGSSAE 60
           MASSLCFQLHTCSQLRCIAAAKAAAAAAPSSVATANRTLSLLVKRPFL SFCLPSGSSAE
Sbjct: 1   MASSLCFQLHTCSQLRCIAAAKAAAAAAPSSVATANRTLSLLVKRPFLHSFCLPSGSSAE 60

Query: 61  LCERQQLRSPELVALEYADLNLSHKIFEDSGHVRIRQHVNPLSSSFSVPTPAPNWDEVFK 120
           LCERQQLRSPELVALEYADLNLSHKIFEDSGHVRIRQHVNPLSSSFSVPTPAPNWDEVFK
Sbjct: 61  LCERQQLRSPELVALEYADLNLSHKIFEDSGHVRIRQHVNPLSSSFSVPTPAPNWDEVFK 120

Query: 121 DPTLPLMVDIGSGSGRFLLWLAKKHLDSRNFLGLEIRQKLVKRADVWVKELGFNNVHFMF 180
           DPTLPLMVDIGSGSGRFLLWLAKKHLDSRNFLGLEIRQKLVKRADVWVKELGFNNVHFMF
Sbjct: 121 DPTLPLMVDIGSGSGRFLLWLAKKHLDSRNFLGLEIRQKLVKRADVWVKELGFNNVHFMF 180

Query: 181 ANATISFKQIISSYPGPLMLVSILCPDPHFKKRHHKRRVLQKPLVDSIIDSLMPGGQVFI 240
           ANATISFKQIISSYPGPLMLVSILCPDPHFKKRHHKRRVLQKPLVDSIIDSL PGGQVFI
Sbjct: 181 ANATISFKQIISSYPGPLMLVSILCPDPHFKKRHHKRRVLQKPLVDSIIDSLKPGGQVFI 240

Query: 241 QSDVLEVAVDMRLWFDSVPDVLKHVDVLDQSLLCDKEGWLLKNPMGIRTEREVHAESEGA 300
           QSDVLEVAVDMRLWFDSVP+VLKHVDVLDQSLLCDK+GWLL NPMGIRTERE+HAESEGA
Sbjct: 241 QSDVLEVAVDMRLWFDSVPNVLKHVDVLDQSLLCDKDGWLLNNPMGIRTEREIHAESEGA 300

Query: 301 NIYRRMYQKYVCI 314
           NIYRRMYQKYVCI
Sbjct: 301 NIYRRMYQKYVCI 313

[Close]

BLAST of Carg14852 vs. NCBI nr
Match: KAG6582546.1 (hypothetical protein SDJN03_22548, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 619.8 bits (1597), Expect = 1.3e-173
Identity = 307/313 (98.08%), Postives = 310/313 (99.04%), Query Frame = 0


Query: 1   MASSLCFQLHTCSQLRCIAAAKAAAAAAPSSVATANRTLSLLVKRPFLQSFCLPSGSSAE 60
           MASSLCFQLHTCSQLRCIAAAKAAAAAAPSSVATANRTLSLLVKRPFLQSFCLPS SSAE
Sbjct: 1   MASSLCFQLHTCSQLRCIAAAKAAAAAAPSSVATANRTLSLLVKRPFLQSFCLPSDSSAE 60

Query: 61  LCERQQLRSPELVALEYADLNLSHKIFEDSGHVRIRQHVNPLSSSFSVPTPAPNWDEVFK 120
           LCERQQLRSPELVALEYADLNLSHKIFEDSGHVRIRQHVNPLSSSFSVPTPAPNWDEVFK
Sbjct: 61  LCERQQLRSPELVALEYADLNLSHKIFEDSGHVRIRQHVNPLSSSFSVPTPAPNWDEVFK 120

Query: 121 DPTLPLMVDIGSGSGRFLLWLAKKHLDSRNFLGLEIRQKLVKRADVWVKELGFNNVHFMF 180
           DP LPLMVDIGSGSGRFLLWLAKKHLDSRNFLGLEIRQKLVKRADVWVKELGFNNVHFMF
Sbjct: 121 DPKLPLMVDIGSGSGRFLLWLAKKHLDSRNFLGLEIRQKLVKRADVWVKELGFNNVHFMF 180

Query: 181 ANATISFKQIISSYPGPLMLVSILCPDPHFKKRHHKRRVLQKPLVDSIIDSLMPGGQVFI 240
           ANATISFKQIISSYPGPLMLVSILCPDPHFKKRHHKRRVLQKPLVDSIIDSLMPGGQVFI
Sbjct: 181 ANATISFKQIISSYPGPLMLVSILCPDPHFKKRHHKRRVLQKPLVDSIIDSLMPGGQVFI 240

Query: 241 QSDVLEVAVDMRLWFDSVPDVLKHVDVLDQSLLCDKEGWLLKNPMGIRTEREVHAESEGA 300
           QSDVLEVAVDMRLWFDSVP+VLKH DVLDQSLLCDK+GWLLKNPMGIRTERE+HAESEGA
Sbjct: 241 QSDVLEVAVDMRLWFDSVPNVLKHADVLDQSLLCDKDGWLLKNPMGIRTEREIHAESEGA 300

Query: 301 NIYRRMYQKYVCI 314
           NIYRRMYQKYVCI
Sbjct: 301 NIYRRMYQKYVCI 313

[Close]

BLAST of Carg14852 vs. ExPASy Swiss-Prot
Match: Q2JJQ0 (tRNA (guanine-N(7)-)-methyltransferase OS=Synechococcus sp. (strain JA-2-3B'a(2-13)) OX=321332 GN=trmB PE=3 SV=1)

HSP 1 Score: 183.0 bits (463), Expect = 5.4e-45
Identity = 102/214 (47.66%), Postives = 133/214 (62.15%), Query Frame = 0


Query: 91  GHVRIRQHVNPLSSSFSVPTPAPNWDEVFKDPTLPLMVDIGSGSGRFLLWLAKKHLDSRN 150
           GH R+RQHVNPL + +  P P P+W+ V++    P  +DIG+GSGRFLL +A++  D  N
Sbjct: 35  GH-RVRQHVNPLQAQYQQPAPPPHWERVYRRLGQPFHLDIGTGSGRFLLRIAQEQPD-WN 94

Query: 151 FLGLEIRQKLVKRADVWVKELGFNNVHFMFANATISFKQIISSYPGPLMLVSILCPDPHF 210
           FLG+EIRQ LV+RA+ W  ELG +NVHF+FAN  +S + + +  PG L  V+IL PDP F
Sbjct: 95  FLGVEIRQALVERANAWRDELGLDNVHFLFANINVSLRHLFA--PGDLSRVTILFPDPWF 154

Query: 211 KKRHHKRRVLQKPLVDSIIDSLMPGGQVFIQSDVLEVAVDMRLWFDSVPDVLKHVDVLDQ 270
           KKRHHKRR++Q  LV  +   L PG  VF+QSD+ EVA +M   F      L+H    D 
Sbjct: 155 KKRHHKRRIVQPQLVADLALLLRPGSPVFLQSDIQEVAEEMAARF------LEHPQFWD- 214

Query: 271 SLLCDKEGWLLKNPMGIRTEREVHAESEGANIYR 305
                 +G L  NP+GI TERE         IYR
Sbjct: 215 ----PHQGPLDSNPLGIPTEREWQCLQLDLPIYR 233

[Close]

BLAST of Carg14852 vs. ExPASy Swiss-Prot
Match: Q2JRH1 (tRNA (guanine-N(7)-)-methyltransferase OS=Synechococcus sp. (strain JA-3-3Ab) OX=321327 GN=trmB PE=3 SV=1)

HSP 1 Score: 182.2 bits (461), Expect = 9.2e-45
Identity = 102/211 (48.34%), Postives = 132/211 (62.56%), Query Frame = 0


Query: 94  RIRQHVNPLSSSFSVPTPAPNWDEVFKDPTLPLMVDIGSGSGRFLLWLAKKHLDSRNFLG 153
           R+RQHVNPL + +  PTP P+W+ V++    PL +DIGSGSGRFLL +A++  D  NFLG
Sbjct: 34  RLRQHVNPLQAQYQQPTPPPHWERVYRRLGQPLHLDIGSGSGRFLLRMAQEQPD-WNFLG 93

Query: 154 LEIRQKLVKRADVWVKELGFNNVHFMFANATISFKQIISSYPGPLMLVSILCPDPHFKKR 213
           +EIRQ LV+RA+ W  ELG +NVHF+FAN  +S + + +  PG L  V+I  PDP FKKR
Sbjct: 94  VEIRQPLVERANAWRDELGLDNVHFLFANINVSLRHLFA--PGDLSRVTIQFPDPWFKKR 153

Query: 214 HHKRRVLQKPLVDSIIDSLMPGGQVFIQSDVLEVAVDMRLWFDSVPDVLKHVDVLDQSLL 273
           HHKRR++Q  LV  +   L PG  VF+QSDV EVA +M      V   L+H    +    
Sbjct: 154 HHKRRIVQPRLVADLALLLQPGSPVFLQSDVREVAEEM------VDRFLEHPQFWN---- 213

Query: 274 CDKEGWLLKNPMGIRTEREVHAESEGANIYR 305
              +G +  NP GI TERE      G  IYR
Sbjct: 214 -PYQGPIDDNPFGIPTEREWQCLQLGLPIYR 230

[Close]

BLAST of Carg14852 vs. ExPASy Swiss-Prot
Match: P73161 (tRNA (guanine-N(7)-)-methyltransferase OS=Synechocystis sp. (strain PCC 6803 / Kazusa) OX=1111708 GN=trmB PE=3 SV=2)

HSP 1 Score: 166.8 bits (421), Expect = 4.0e-40
Identity = 93/217 (42.86%), Postives = 126/217 (58.06%), Query Frame = 0


Query: 93  VRIRQHVNPLSSSFSVPTPAPNWDEVFKDPTLPLMVDIGSGSGRFLLWLAKKHLDSRNFL 152
           VRIRQHVNPLS  +      P+W  V+ D   PL +DIG   GRF L +A++H D  NFL
Sbjct: 4   VRIRQHVNPLSEKYRQVLACPDWATVYDDVQRPLHLDIGCARGRFPLKMAQQHPD-WNFL 63

Query: 153 GLEIRQKLVKRADVWVKELGFNNVHFMFANATISFKQIISSYPGPLMLVSILCPDPHFKK 212
           G+EIRQ LV  A+     LG  N+H++F N  +  ++  S++P  L  VSI  PDP FK+
Sbjct: 64  GVEIRQPLVLEANETGDRLGLKNLHYLFGNINVEPEKFFSAFPPTLQRVSIQFPDPWFKQ 123

Query: 213 RHHKRRVLQKPLVDSIIDSLMPGGQVFIQSDVLEVAVDMRLWFDSVPDVLKHVDVLDQSL 272
           RH+KRRV Q  LV +I ++L PGG+V +QSDV  VA DMR  F             D  +
Sbjct: 124 RHNKRRVAQPELVTAIANALPPGGEVLLQSDVEPVAQDMRDRFAE----------NDNFV 183

Query: 273 LCDKEGWLLKNPMGIRTEREVHAESEGANIYRRMYQK 310
                 WL +NP+G+ TERE+   +    +YR + Q+
Sbjct: 184 FTHDTPWLAENPLGVPTEREIACFNLQRPVYRCLLQR 209

[Close]

BLAST of Carg14852 vs. ExPASy Swiss-Prot
Match: P72546 (tRNA (guanine-N(7)-)-methyltransferase OS=Synechococcus elongatus (strain PCC 7942 / FACHB-805) OX=1140 GN=trmB PE=3 SV=2)

HSP 1 Score: 161.4 bits (407), Expect = 1.7e-38
Identity = 91/216 (42.13%), Postives = 128/216 (59.26%), Query Frame = 0


Query: 93  VRIRQHVNPLSSSFSVPTPAPNWDEVFKDPTLPLMVDIGSGSGRFLLWLAKKHLDSRNFL 152
           VR+RQHVNPLS  F V T  P+W +V+ D   PL +DIG   GRFLL +A       N+L
Sbjct: 4   VRVRQHVNPLSQKFQVVTTWPDWQQVYADCDRPLHLDIGCARGRFLLAMATAQ-PEWNYL 63

Query: 153 GLEIRQKLVKRADVWVKELGFNNVHFMFANATISFKQIISSYP-GPLMLVSILCPDPHFK 212
           GLEIR+ LV  A+   +E    N+++ F+NA +  + ++ S P G L  VSI  PDP FK
Sbjct: 64  GLEIREPLVDEANAIARERELTNLYYHFSNANLDLEPLLRSLPTGILQRVSIQFPDPWFK 123

Query: 213 KRHHKRRVLQKPLVDSIIDSLMPGGQVFIQSDVLEVAVDMRLWFDSVPDVLKHVDVLDQS 272
           KRH KRRV+Q  LV ++  +L  G +VF+QSDVLEV  +M   F + P   +    LD  
Sbjct: 124 KRHQKRRVVQPELVQALATALPAGAEVFLQSDVLEVQAEMCEHFAAEPRFQR--TCLD-- 183

Query: 273 LLCDKEGWLLKNPMGIRTEREVHAESEGANIYRRMY 308
                  WL +NP+ + TERE+  +++   +YR ++
Sbjct: 184 -------WLPENPLPVPTEREIAVQNKQLPVYRALF 207

[Close]

BLAST of Carg14852 vs. ExPASy Swiss-Prot
Match: Q5N2X5 (tRNA (guanine-N(7)-)-methyltransferase OS=Synechococcus sp. (strain ATCC 27144 / PCC 6301 / SAUG 1402/1) OX=269084 GN=trmB PE=3 SV=1)

HSP 1 Score: 161.4 bits (407), Expect = 1.7e-38
Identity = 91/216 (42.13%), Postives = 128/216 (59.26%), Query Frame = 0


Query: 93  VRIRQHVNPLSSSFSVPTPAPNWDEVFKDPTLPLMVDIGSGSGRFLLWLAKKHLDSRNFL 152
           VR+RQHVNPLS  F V T  P+W +V+ D   PL +DIG   GRFLL +A       N+L
Sbjct: 4   VRVRQHVNPLSQKFQVVTTWPDWQQVYADCDRPLHLDIGCARGRFLLAMATAQ-PEWNYL 63

Query: 153 GLEIRQKLVKRADVWVKELGFNNVHFMFANATISFKQIISSYP-GPLMLVSILCPDPHFK 212
           GLEIR+ LV  A+   +E    N+++ F+NA +  + ++ S P G L  VSI  PDP FK
Sbjct: 64  GLEIREPLVDEANAIARERELTNLYYHFSNANLDLEPLLRSLPTGILQRVSIQFPDPWFK 123

Query: 213 KRHHKRRVLQKPLVDSIIDSLMPGGQVFIQSDVLEVAVDMRLWFDSVPDVLKHVDVLDQS 272
           KRH KRRV+Q  LV ++  +L  G +VF+QSDVLEV  +M   F + P   +    LD  
Sbjct: 124 KRHQKRRVVQPELVQALATALPAGAEVFLQSDVLEVQAEMCEHFAAEPRFQR--TCLD-- 183

Query: 273 LLCDKEGWLLKNPMGIRTEREVHAESEGANIYRRMY 308
                  WL +NP+ + TERE+  +++   +YR ++
Sbjct: 184 -------WLPENPLPVPTEREIAVQNKQLPVYRALF 207

[Close]

BLAST of Carg14852 vs. ExPASy TrEMBL
Match: A0A6J1E9S1 (uncharacterized protein LOC111432133 OS=Cucurbita moschata OX=3662 GN=LOC111432133 PE=4 SV=1)

HSP 1 Score: 623.6 bits (1607), Expect = 4.4e-175
Identity = 309/313 (98.72%), Postives = 312/313 (99.68%), Query Frame = 0


Query: 1   MASSLCFQLHTCSQLRCIAAAKAAAAAAPSSVATANRTLSLLVKRPFLQSFCLPSGSSAE 60
           MASSLCFQLHTCSQLRCIAAAKAAAAAAPSSVATANRTLSLLVKRPFLQSFCLPSGSSAE
Sbjct: 1   MASSLCFQLHTCSQLRCIAAAKAAAAAAPSSVATANRTLSLLVKRPFLQSFCLPSGSSAE 60

Query: 61  LCERQQLRSPELVALEYADLNLSHKIFEDSGHVRIRQHVNPLSSSFSVPTPAPNWDEVFK 120
           LCERQQLRSPELVALEYADLNLSHKIFEDSGHVRIRQHVNPLSSSFSVPTPAPNWDEVFK
Sbjct: 61  LCERQQLRSPELVALEYADLNLSHKIFEDSGHVRIRQHVNPLSSSFSVPTPAPNWDEVFK 120

Query: 121 DPTLPLMVDIGSGSGRFLLWLAKKHLDSRNFLGLEIRQKLVKRADVWVKELGFNNVHFMF 180
           DPTLPLMVDIGSGSGRFLLWLAKKHLDSRNFLGLEIRQKLVKRADVWVKELGFNNVHFMF
Sbjct: 121 DPTLPLMVDIGSGSGRFLLWLAKKHLDSRNFLGLEIRQKLVKRADVWVKELGFNNVHFMF 180

Query: 181 ANATISFKQIISSYPGPLMLVSILCPDPHFKKRHHKRRVLQKPLVDSIIDSLMPGGQVFI 240
           ANATISFKQIISSYPGPLMLVSILCPDPHFKKRHHKRRVLQKPLVDSIIDSLMPGGQVFI
Sbjct: 181 ANATISFKQIISSYPGPLMLVSILCPDPHFKKRHHKRRVLQKPLVDSIIDSLMPGGQVFI 240

Query: 241 QSDVLEVAVDMRLWFDSVPDVLKHVDVLDQSLLCDKEGWLLKNPMGIRTEREVHAESEGA 300
           QSDVLEVAVDMRLWFDSVP+VLKHVDV DQSLLCDK+GWLLKNPMGIRTERE+HAESEGA
Sbjct: 241 QSDVLEVAVDMRLWFDSVPNVLKHVDVHDQSLLCDKDGWLLKNPMGIRTEREIHAESEGA 300

Query: 301 NIYRRMYQKYVCI 314
           NIYRRMYQKYVCI
Sbjct: 301 NIYRRMYQKYVCI 313

[Close]

BLAST of Carg14852 vs. ExPASy TrEMBL
Match: A0A6J1ITE1 (uncharacterized protein LOC111479752 OS=Cucurbita maxima OX=3661 GN=LOC111479752 PE=4 SV=1)

HSP 1 Score: 620.2 bits (1598), Expect = 4.9e-174
Identity = 307/313 (98.08%), Postives = 310/313 (99.04%), Query Frame = 0


Query: 1   MASSLCFQLHTCSQLRCIAAAKAAAAAAPSSVATANRTLSLLVKRPFLQSFCLPSGSSAE 60
           MASSLCFQLHTCSQLRCIAAAKAAAAAAPSSVATANRTLSLLVKRPFL SFCLPSGSSAE
Sbjct: 1   MASSLCFQLHTCSQLRCIAAAKAAAAAAPSSVATANRTLSLLVKRPFLHSFCLPSGSSAE 60

Query: 61  LCERQQLRSPELVALEYADLNLSHKIFEDSGHVRIRQHVNPLSSSFSVPTPAPNWDEVFK 120
           LCERQQLRSPELVALEYADLNLSHKIFEDSGHVRIRQHVNPLSSSFSVPTPAPNWDEVFK
Sbjct: 61  LCERQQLRSPELVALEYADLNLSHKIFEDSGHVRIRQHVNPLSSSFSVPTPAPNWDEVFK 120

Query: 121 DPTLPLMVDIGSGSGRFLLWLAKKHLDSRNFLGLEIRQKLVKRADVWVKELGFNNVHFMF 180
           DPTLPLMVDIGSGSGRFLLWLAKKHLDSRNFLGLEIRQKLVKRADVWVKELGFNNVHFMF
Sbjct: 121 DPTLPLMVDIGSGSGRFLLWLAKKHLDSRNFLGLEIRQKLVKRADVWVKELGFNNVHFMF 180

Query: 181 ANATISFKQIISSYPGPLMLVSILCPDPHFKKRHHKRRVLQKPLVDSIIDSLMPGGQVFI 240
           ANATISFKQIISSYPGPLMLVSILCPDPHFKKRHHKRRVLQKPLVDSIIDSL PGGQVFI
Sbjct: 181 ANATISFKQIISSYPGPLMLVSILCPDPHFKKRHHKRRVLQKPLVDSIIDSLKPGGQVFI 240

Query: 241 QSDVLEVAVDMRLWFDSVPDVLKHVDVLDQSLLCDKEGWLLKNPMGIRTEREVHAESEGA 300
           QSDVLEVAVDMRLWFDSVP+VLKHVDVLDQSLLCDK+GWLL NPMGIRTERE+HAESEGA
Sbjct: 241 QSDVLEVAVDMRLWFDSVPNVLKHVDVLDQSLLCDKDGWLLNNPMGIRTEREIHAESEGA 300

Query: 301 NIYRRMYQKYVCI 314
           NIYRRMYQKYVCI
Sbjct: 301 NIYRRMYQKYVCI 313

[Close]

BLAST of Carg14852 vs. ExPASy TrEMBL
Match: A0A6J1CZT2 (uncharacterized protein LOC111015991 OS=Momordica charantia OX=3673 GN=LOC111015991 PE=4 SV=1)

HSP 1 Score: 535.8 bits (1379), Expect = 1.2e-148
Identity = 266/312 (85.26%), Postives = 285/312 (91.35%), Query Frame = 0


Query: 1   MASSLCFQLHTCSQLRCIAAAKAAAAAAPSSVATANRTLS--LLVKRPFLQSFCLPSGSS 60
           MA SLCFQLHTCSQL CIAAAK   AAAPSSVATANRTLS  L ++RPFL SFC+  G S
Sbjct: 1   MAPSLCFQLHTCSQLGCIAAAK---AAAPSSVATANRTLSPFLRLRRPFLHSFCVRLGPS 60

Query: 61  AELCERQQLRSPELVALEYADLNLSHKIFEDSGHVRIRQHVNPLSSSFSVPTPAPNWDEV 120
           AE+ E Q LRSPELVALEYADLNL H+IFEDSGHVRIRQHVNPLSSSF+VP PAPNW+EV
Sbjct: 61  AEVFEPQHLRSPELVALEYADLNLPHRIFEDSGHVRIRQHVNPLSSSFAVPAPAPNWNEV 120

Query: 121 FKDPTLPLMVDIGSGSGRFLLWLAKKHLDSRNFLGLEIRQKLVKRADVWVKELGFNNVHF 180
           FKDPTLPLMVDIGSGSGRFLLWLAK+HLD RNFLGLEIR KLVKRAD+WVKELG +NVHF
Sbjct: 121 FKDPTLPLMVDIGSGSGRFLLWLAKRHLDMRNFLGLEIRHKLVKRADIWVKELGLSNVHF 180

Query: 181 MFANATISFKQIISSYPGPLMLVSILCPDPHFKKRHHKRRVLQKPLVDSIIDSLMPGGQV 240
           MFANATISFKQIISSYPGPLMLVSILCPDPHFKKRHHKRRV+QKPLV+SI+DSL PGGQV
Sbjct: 181 MFANATISFKQIISSYPGPLMLVSILCPDPHFKKRHHKRRVVQKPLVESIVDSLTPGGQV 240

Query: 241 FIQSDVLEVAVDMRLWFDSVPDVLKHVDVLDQSLLCDKEGWLLKNPMGIRTEREVHAESE 300
            IQSDVLEVAVDMRLWFDS  DVLKH+DVLD S+LCD++GWLL NPMGIRTERE+HAESE
Sbjct: 241 LIQSDVLEVAVDMRLWFDSEADVLKHIDVLDPSVLCDEDGWLLNNPMGIRTEREIHAESE 300

Query: 301 GANIYRRMYQKY 311
           GA IYRRMYQK+
Sbjct: 301 GAKIYRRMYQKF 309

[Close]

BLAST of Carg14852 vs. ExPASy TrEMBL
Match: A0A1S3AVT2 (tRNA (Guanine-N(7)-)-methyltransferase OS=Cucumis melo OX=3656 GN=LOC103483376 PE=4 SV=1)

HSP 1 Score: 530.0 bits (1364), Expect = 6.6e-147
Identity = 262/309 (84.79%), Postives = 282/309 (91.26%), Query Frame = 0


Query: 1   MASSLCFQLHTCSQLRCIAAAKAAAAAAPSSVATANRTLSLLVKRPFLQSFCLPSGSSAE 60
           MASSLC  L TCS LRC+AAAK    AAPS VATAN+ LS   KRPFL++F LPSGSSA+
Sbjct: 1   MASSLCLHLQTCSHLRCLAAAK---PAAPSVVATANKILS---KRPFLRNFSLPSGSSAD 60

Query: 61  LCERQQLRSPELVALEYADLNLSHKIFEDSGHVRIRQHVNPLSSSFSVPTPAPNWDEVFK 120
           LCERQ LRS ELVALEYADLNLSHK+ ED GHVRIRQHVNPLSSSFSVPTPAPNWDEVF+
Sbjct: 61  LCERQPLRSSELVALEYADLNLSHKMVEDLGHVRIRQHVNPLSSSFSVPTPAPNWDEVFR 120

Query: 121 DPTLPLMVDIGSGSGRFLLWLAKKHLDSRNFLGLEIRQKLVKRADVWVKELGFNNVHFMF 180
           DPTLPLMVDIGSGSGRFLLWLAK HL+SRNFLGLEIRQKLVKRA  WVKELGF+NVHFMF
Sbjct: 121 DPTLPLMVDIGSGSGRFLLWLAKSHLESRNFLGLEIRQKLVKRAGFWVKELGFSNVHFMF 180

Query: 181 ANATISFKQIISSYPGPLMLVSILCPDPHFKKRHHKRRVLQKPLVDSIIDSLMPGGQVFI 240
           ANATISFKQIISSYPGPL+LVSILCPDPHFKKRHHKRRVLQKPLV+SI+DSLMPGGQVFI
Sbjct: 181 ANATISFKQIISSYPGPLLLVSILCPDPHFKKRHHKRRVLQKPLVESIVDSLMPGGQVFI 240

Query: 241 QSDVLEVAVDMRLWFDSVPDVLKHVDVLDQSLLCDKEGWLLKNPMGIRTEREVHAESEGA 300
           QSDVLEVA DMRLWFDS  D+L+H+D +D+S+LCDK+GWLL NPMGIRTERE+HAESEGA
Sbjct: 241 QSDVLEVAADMRLWFDSEVDILEHIDKIDKSVLCDKDGWLLNNPMGIRTEREIHAESEGA 300

Query: 301 NIYRRMYQK 310
           NIYRRMY K
Sbjct: 301 NIYRRMYSK 303

[Close]

BLAST of Carg14852 vs. ExPASy TrEMBL
Match: A0A5A7U1F8 (tRNA (Guanine-N(7)-)-methyltransferase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold434G003710 PE=4 SV=1)

HSP 1 Score: 529.3 bits (1362), Expect = 1.1e-146
Identity = 261/309 (84.47%), Postives = 282/309 (91.26%), Query Frame = 0


Query: 1   MASSLCFQLHTCSQLRCIAAAKAAAAAAPSSVATANRTLSLLVKRPFLQSFCLPSGSSAE 60
           MASSLC  L TCS LRC+ AAK    AAPS VATAN+ LS   KRPFL++F LPSGSSA+
Sbjct: 1   MASSLCLHLQTCSHLRCLGAAK---PAAPSVVATANKILS---KRPFLRNFSLPSGSSAD 60

Query: 61  LCERQQLRSPELVALEYADLNLSHKIFEDSGHVRIRQHVNPLSSSFSVPTPAPNWDEVFK 120
           LCERQ LRS ELVALEYADLNLSHK+ ED GHVRIRQHVNPLSSSFSVPTPAPNWDEVF+
Sbjct: 61  LCERQPLRSSELVALEYADLNLSHKMVEDLGHVRIRQHVNPLSSSFSVPTPAPNWDEVFR 120

Query: 121 DPTLPLMVDIGSGSGRFLLWLAKKHLDSRNFLGLEIRQKLVKRADVWVKELGFNNVHFMF 180
           DPTLPLMVDIGSGSGRFLLWLAK+HL+SRNFLGLEIRQKLVKRA  WVKELGF+NVHFMF
Sbjct: 121 DPTLPLMVDIGSGSGRFLLWLAKRHLESRNFLGLEIRQKLVKRAGFWVKELGFSNVHFMF 180

Query: 181 ANATISFKQIISSYPGPLMLVSILCPDPHFKKRHHKRRVLQKPLVDSIIDSLMPGGQVFI 240
           ANATISFKQIISSYPGPL+LVSILCPDPHFKKRHHKRRVLQKPLV+SI+DSLMPGGQVFI
Sbjct: 181 ANATISFKQIISSYPGPLLLVSILCPDPHFKKRHHKRRVLQKPLVESIVDSLMPGGQVFI 240

Query: 241 QSDVLEVAVDMRLWFDSVPDVLKHVDVLDQSLLCDKEGWLLKNPMGIRTEREVHAESEGA 300
           QSDVLEVA DMRLWFDS  D+L+H+D +D+S+LCDK+GWLL NPMGIRTERE+HAESEGA
Sbjct: 241 QSDVLEVAADMRLWFDSEVDILEHIDKIDKSVLCDKDGWLLNNPMGIRTEREIHAESEGA 300

Query: 301 NIYRRMYQK 310
           NIYRRMY K
Sbjct: 301 NIYRRMYSK 303

[Close]

BLAST of Carg14852 vs. TAIR 10
Match: AT5G17660.1 (tRNA (guanine-N-7) methyltransferase )

HSP 1 Score: 369.0 bits (946), Expect = 3.8e-102
Identity = 177/256 (69.14%), Postives = 214/256 (83.59%), Query Frame = 0


Query: 55  SGSSAELC-ERQQLRSPELVALEYADLNLSHKIFEDSGHVRIRQHVNPLSSSFSVPTPAP 114
           S +S  +C + + LRS ELV LEYA+LNL+HKI E+ GHVRIRQHVNPLSSSFS P P P
Sbjct: 55  SSASTSVCVDFKDLRSNELVELEYAELNLNHKISEEVGHVRIRQHVNPLSSSFSKPAPVP 114

Query: 115 NWDEVFKDPTLPLMVDIGSGSGRFLLWLAKKHLDSRNFLGLEIRQKLVKRADVWVKELGF 174
            WDEV+KDP+LPLMVDIGSGSGRFLLW A K+++SRN+LGLEIRQKLVKRA+ WV ELG 
Sbjct: 115 VWDEVYKDPSLPLMVDIGSGSGRFLLWQANKNVESRNYLGLEIRQKLVKRANFWVNELGL 174

Query: 175 NNVHFMFANATISFKQIISSYPGPLMLVSILCPDPHFKKRHHKRRVLQKPLVDSIIDSLM 234
           +NVHF+FANA +SF+ +ISSYPGPL +VSILCPDPHFKKRH KRRV+QKPLV+SI+ +L 
Sbjct: 175 SNVHFIFANAMVSFEHLISSYPGPLEIVSILCPDPHFKKRHQKRRVVQKPLVNSILQNLK 234

Query: 235 PGGQVFIQSDVLEVAVDMRLWFDSVPDVLKHVDVLDQSLLCDKEGWLLKNPMGIRTEREV 294
           PGG++F+QSDVL+VA DMR   D   +VL+H+D +D      ++GWL +NPMGIRTERE+
Sbjct: 235 PGGKIFVQSDVLDVAQDMRDQLDEESNVLQHMDTVD-----TEDGWLTENPMGIRTEREI 294

Query: 295 HAESEGANIYRRMYQK 310
           HAE EGA IYRR+YQK
Sbjct: 295 HAEFEGARIYRRLYQK 305

[Close]

BLAST of Carg14852 vs. TAIR 10
Match: AT5G24840.1 (tRNA (guanine-N-7) methyltransferase )

HSP 1 Score: 65.9 bits (159), Expect = 6.8e-11
Identity = 53/182 (29.12%), Postives = 82/182 (45.05%), Query Frame = 0


Query: 94  RIRQHVNPLSSS-FSVPTPAPNWDEVFKDPTL----------PLMVDIGSGSGRFLLWLA 153
           R R H NPLS S F +P    + D     P                DIG G G  L+ LA
Sbjct: 23  RARAHSNPLSDSHFPIPISPAHVDYSLHFPKFVEADNKFIKKVEFADIGCGFGGLLISLA 82

Query: 154 KKHLDSRNFLGLEIRQKLVKRADVWVKELGFNNVHFMFANATI----SFKQIISSY-PGP 213
               D+   +G+E+R K+ +     +  L   +    + N ++    S K I + +  G 
Sbjct: 83  TLFPDTL-MIGMELRDKVTEYVKERILALRRTSSEGQYENISVVRTNSMKYIPNYFEKGQ 142

Query: 214 LMLVSILCPDPHFKKRHHKRRVLQKPLVDSIIDSLMPGGQVFIQSDVLEVAVDMRLWFDS 260
           L  +  L PDPHFK+++H+RRV+   L+D     L  GG ++  +DV E+   M+   + 
Sbjct: 143 LSKMFFLFPDPHFKEKNHRRRVISTHLLDEYAYVLRAGGIIYTITDVEELGEWMKSCLEK 202

The following BLAST results are available for this feature:

BLAST of Carg14852 vs. NCBI nr


Match Name	E-value	Identity	Description
KAG7018929.1	1.3e-176	100.00	trmB [Cucurbita argyrosperma subsp. argyrosperma]	[more]
XP_023527130.1	2.4e-175	98.72	uncharacterized protein LOC111790461 [Cucurbita pepo subsp. pepo]	[more]
XP_022924717.1	9.1e-175	98.72	uncharacterized protein LOC111432133 [Cucurbita moschata]	[more]
XP_022980361.1	1.0e-173	98.08	uncharacterized protein LOC111479752 [Cucurbita maxima]	[more]
KAG6582546.1	1.3e-173	98.08	hypothetical protein SDJN03_22548, partial [Cucurbita argyrosperma subsp. sorori...	[more]

BLAST of Carg14852 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q2JJQ0	5.4e-45	47.66	tRNA (guanine-N(7)-)-methyltransferase OS=Synechococcus sp. (strain JA-2-3B'a(2-...	[more]
Q2JRH1	9.2e-45	48.34	tRNA (guanine-N(7)-)-methyltransferase OS=Synechococcus sp. (strain JA-3-3Ab) OX...	[more]
P73161	4.0e-40	42.86	tRNA (guanine-N(7)-)-methyltransferase OS=Synechocystis sp. (strain PCC 6803 / K...	[more]
P72546	1.7e-38	42.13	tRNA (guanine-N(7)-)-methyltransferase OS=Synechococcus elongatus (strain PCC 79...	[more]
Q5N2X5	1.7e-38	42.13	tRNA (guanine-N(7)-)-methyltransferase OS=Synechococcus sp. (strain ATCC 27144 /...	[more]

BLAST of Carg14852 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1E9S1	4.4e-175	98.72	uncharacterized protein LOC111432133 OS=Cucurbita moschata OX=3662 GN=LOC1114321...	[more]
A0A6J1ITE1	4.9e-174	98.08	uncharacterized protein LOC111479752 OS=Cucurbita maxima OX=3661 GN=LOC111479752...	[more]
A0A6J1CZT2	1.2e-148	85.26	uncharacterized protein LOC111015991 OS=Momordica charantia OX=3673 GN=LOC111015...	[more]
A0A1S3AVT2	6.6e-147	84.79	tRNA (Guanine-N(7)-)-methyltransferase OS=Cucumis melo OX=3656 GN=LOC103483376 P...	[more]
A0A5A7U1F8	1.1e-146	84.47	tRNA (Guanine-N(7)-)-methyltransferase OS=Cucumis melo var. makuwa OX=1194695 GN...	[more]

BLAST of Carg14852 vs. TAIR 10


Match Name	E-value	Identity	Description
AT5G17660.1	3.8e-102	69.14	tRNA (guanine-N-7) methyltransferase	[more]
AT5G24840.1	6.8e-11	29.12	tRNA (guanine-N-7) methyltransferase	[more]

InterPro

Analysis Name: InterPro Annotations of Silver-seed gourd (SMH-JMG-627) v2
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
None	No IPR available	GENE3D	3.40.50.150	Vaccinia Virus protein VP39	coord: 94..311 e-value: 2.6E-53 score: 182.3
None	No IPR available	PANTHER	PTHR23417:SF21	TRNA (GUANINE-N(7)-)-METHYLTRANSFERASE	coord: 55..309
None	No IPR available	CDD	cd02440	AdoMet_MTases	coord: 128..241 e-value: 7.24693E-4 score: 36.6391
IPR003358	tRNA (guanine-N-7) methyltransferase, Trmb type	PFAM	PF02390	Methyltransf_4	coord: 125..304 e-value: 2.3E-32 score: 111.8
IPR003358	tRNA (guanine-N-7) methyltransferase, Trmb type	PANTHER	PTHR23417	3-DEOXY-D-MANNO-OCTULOSONIC-ACID TRANSFERASE/TRNA GUANINE-N 7 - -METHYLTRANSFERASE	coord: 55..309
IPR003358	tRNA (guanine-N-7) methyltransferase, Trmb type	PROSITE	PS51625	SAM_MT_TRMB	coord: 83..309 score: 52.839981
IPR029063	S-adenosyl-L-methionine-dependent methyltransferase	SUPERFAMILY	53335	S-adenosyl-L-methionine-dependent methyltransferases	coord: 125..308

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Carg14852-RA	Carg14852-RA	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0106004	tRNA (guanine-N7)-methylation
biological_process	GO:0006400	tRNA modification
cellular_component	GO:0043527	tRNA methyltransferase complex
molecular_function	GO:0008176	tRNA (guanine-N7-)-methyltransferase activity