Carg14778 (gene) Silver-seed gourd (SMH-JMG-627) v2

Overview
NameCarg14778
Typegene
OrganismCucurbita argyrosperma subsp. argyrosperma cv. SMH-JMG-627 (Silver-seed gourd (SMH-JMG-627) v2)
DescriptionTrypsin family protein
LocationCarg_Chr01: 10377662 .. 10381463 (+)
RNA-Seq ExpressionCarg14778
SyntenyCarg14778
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
GTGAACTGCATAATGGAGCAGACAAGACACAACAGCAGGATTAACTGCTCTGGTTCGACACCATCCGAGGAATCAGCTCTGGATCTTGAAAGGAATTGCTACGGTCACTCTAATTTACCTTCCTTCAGTCCACCTACACTTCAGCCATTTGCATCAGCTGGACAGCATTGTGAGAGCAATGCAGCTTACTTTTCATGGCCGACGCCCATTCGATTAAGCGTTGCAGCCGAGGAGAGGGCAAACTACTTCGCAAATCTTCAAAAAGGGGTGCTACCAGATATCCTTCGTCCATTACCAAAAGGGCAGCGAGCCACCACGTTACTCGAGCTCATGACAATACGAGCCTTCCATAGCAAGATCCTACGTTGTTACAGTCTCGGAACGGCGATCGGATTCCGTATCCAAAAGGGTGTGCTGACCGACATTCCTGCTATTCTTGTTTTTGTTTCCAGGAAAGTTCATAAGCAATGGCTTAGTCCTATCCAATGCCTGCCCACCGCGTTGGAGGTTTGAAGAAGTCGTTTTACGTTGGTTGCGAAGTAGCTGTACTAAGTTTCATTCTGAATTCCTTTTTTTGTTTCAGGGGCCGGGAGGTGTGTGGTGTGATGTCGATGTCGTAGAATTCTCGTATTTTGGTGCACCCAACCCTGCTCCTAAAGAACAGCTATACACCGAGATTGTCGATGACCTGCGTGGCTCTGATCTATGCATTGGCTCTGGTTCTCAGGTACATTCATGTTTCTTTGCAACTAGTGACCTGAAAACTTGCATTTATATGAAATATAAAGTTCTTGAATTGAATGAAAAAGACCTCGTAATGAACGAGCGGTAACGGGCAATTCATGTGAGAGACGAGCTTTAGAGATGCAGGGATCCTCCAAGATCATCTTGGTTTGGACCCGAATCTGATATCAAGATGCCATACTTTTTTTCGCTTACGTGTTGGCACTTCCCTGTTGTAATCTTTGCTAATGTTACTACGTGAAGAAGTCGTTTTATATACTCGAAATTGAGATATGTGTCCTAAGTCCGAAGTGTGTCTCTAAACATGCATAGTGTCACAATCGCACCCTTGCAATAGCGGAATAACGATTGTACGGCACTTGTTCTAACCAAGCGAACAAGTCAACCGTGAGAAACTCGGACGTCGCGAACGACGTTGGCGTATGTTCGGGCCTTGAGTCACGTTGTGAGAAAAGGTTTAGAAAATGTGAGCGAGGAAATACATTTGAAAACGATTTGAAGAATTGAAGAAAGCAACGTTATATGACTATAAGCAAATAAAGAAATACCATTGGTTGAATAGCATATAACGTGACTACTAGTCATGTCCCCCTATAGTACATTTTGGGTCATTTTAGCCCTGGCGGGTGTAGACATGGTTTTGGTGAACGGTCATACACCTCCGTATTGACCCAAAGTGAAATGGTGAGGCCCATCTAGGATGAAAGCATGTAAAAGGGGTTTGGAACGGGCATGCAACCATGGACAAGAAAGACCGGGACATCCGTCATGCGGCCATAGGCAACACGCCTTGGACAAGCATGACCGTGACACATAGCAACCGTTTGAACCGATAGCAACTTTTGTGAGTATTGGACCTAAATGGTTAGCTCCAATGGCATTGTCAATAGTTCAAAAGTTGGTAGCATTTGTTCATTTTCACTTCCTTTTTGGTGAAATGGAGATTTTAGGGGGCCTCGCCATCTGAGGCATGGCTTCTGATCAACAAAGTTATAATTATATCAGTAAAATTGACTCTACTTGCTCATCAGGTGGCTAGCCAGGAGACTTATGGGACCTTGGGTGCTATCGTAAGGAGTCAAACGGGCAGTCGACAAGTTGGTTTTCTCACGAACCGTCACGTTGCAGTTGATTTGGATTATCCGAATCAGAAGATGTTTCATCCTCTGCCACCGACGCTTGGGCCGGGGGTGTATCTCGGTGCCGTTGAGAGAGCTACTTCGTTCATCACCGATGAGCTTTGGTATGGAATTTTTGCTGGAATAAATCCAGGTAATGATCCTTGTGCTGCCAAAATAAAATACCCCTGGCAGTCCTGGCTGATACACCGCCTCGTGTCCATCCCCTTTTAGAGTTCAGTCTCCTCGCTGGCACATCGCCCGGTGTCTGGCTTTGATACCATTTATAACAACCCGAGTCCACCGCTAGCAGATATTGTCCTCTTTAGGCTTTCTTTTTCGGGCTTTTCCTCAATGTTTTTAAGACACGTCTGCTAGGGAGAGGTTTCCACACCCTTATAAAGAATGTTTTGTTTTCCTCTCCAACCGATGTGAGATCTCACAGTCCACCCCTCTTCGTGGCCCAGCGTCCTTGCTGGCACTCGTTCCCTTCTCCAATCGATGTGGGACCCCCAATCCACCCCCCTTAGGACCCAGCGTCCTTGCTAGCACACCGCCTCGTGTCCACCGCTCTTCGGGGCTCAACCTCCTCACTGACACATTGCCCGATGTTTGGCTCTAATACCATTTGTAACAACCCAAGTCCACCGCTAGCAGATATTGTTCTCTTTAGGCTTTCCCTCAAGATTTTTAAAACGCTTCTACTAGGGAGAGGTTTCCACACCCTTATAAAGAATGCTTTGTTTTTCTCCCTAACCGATGTGCTCTTTGGGTTGTTACAATTTGCTTACTACACTTCACTGTGTTGTTTCATTTCATCAATCTAAACGTGTGGTAACCCGACGCACTTATTCTAGTTTTGTTAAATTGCAGAGACGTTCGTCAGGGCAGATGGGGCGTTTATTCCTTTTGCTGATGATTTCGACATGTCGACCGTCACTACATCTGTAAAAGGTGTGGGAGAGGTCGGTGATGTGAAGTTTATCGACTTGCAGTCGCCTATCAGTACCCTCATAGGGAAGCAGGTCGTGAAAGTCGGAAGAAGTTCGGGCTTGACTACCGGAACTGTTTTGGCCTATGCTCTCGAGTACAATGACGAGAAAGGAATATGCTTCTTAACCGATTTTCTCGTCGTCGGTGAGAATCAACAGACTTTTGATCTCGAAGGAGATAGCGGAAGTCTCATCATTTTAAAGGGCGAGAATCGAGAGAGTTTGCAACCAATTGGGATTATATGGGGTGGAACTGCTAACCGAGGTCGGCTTAAGTTAAAAGTAGGCCAACCTCCTGAGAATTGGACAAGTGGGGTCGATCTCGGGCGCCTTCTCAATCTGCTTGAACTTGATCTAATCACAACTGATGACGGGTTTAAAGGTTAGTTGTCTTTCTTTTGTGTAGGTTAACTCGTTTCGCTCGCACACCGCGCATTTGATAAAGCTCCATTGTAGATCACATAATGATAGATGTGTTCTGATGAATGTTTTTGTTCTGTTTCTATATTGCTATAAGCAGCAGCAGTGCAAGAACAAAGAACTGTCTCGGCAACAGTTATCGGGTCGATTGTCGGAGACTCCTCTCCTCCCGACACAACGCTGCCAAAGGATAAAAGTGAAGAGAAGTTTGAGCCATTGGGTTTCCAAATCCAGCATATGCCAACAGAAGTAGAACCTTCTTCAGCTAAAGACCGACCACTCCTGGAGACCGAGTTTCATCTCGAAGCTGGGACGAGCACAGCTCCCAGCGTCGAACATCAGTTCATTCCAAGCCTTTTTAGTTGCTCTCCCTCCCTTTCAAACAGCACTCTGGGTCGTGCTGTTTCCCAGAACCTGTCTTCGCTCCGGAGCGACTGCGAAGACATCTCCGTTTCCTTGCACTTGGGTGATCACGAAGCTAAGAGACGACGTTCGGATGCTTCTGTTTCCATGGAAGAGCTGAAATAG

mRNA sequence

GTGAACTGCATAATGGAGCAGACAAGACACAACAGCAGGATTAACTGCTCTGGTTCGACACCATCCGAGGAATCAGCTCTGGATCTTGAAAGGAATTGCTACGGTCACTCTAATTTACCTTCCTTCAGTCCACCTACACTTCAGCCATTTGCATCAGCTGGACAGCATTGTGAGAGCAATGCAGCTTACTTTTCATGGCCGACGCCCATTCGATTAAGCGTTGCAGCCGAGGAGAGGGCAAACTACTTCGCAAATCTTCAAAAAGGGGTGCTACCAGATATCCTTCGTCCATTACCAAAAGGGCAGCGAGCCACCACGTTACTCGAGCTCATGACAATACGAGCCTTCCATAGCAAGATCCTACGTTGTTACAGTCTCGGAACGGCGATCGGATTCCGTATCCAAAAGGGTGTGCTGACCGACATTCCTGCTATTCTTGTTTTTGTTTCCAGGAAAGTTCATAAGCAATGGCTTAGTCCTATCCAATGCCTGCCCACCGCGTTGGAGGGGCCGGGAGGTGTGTGGTGTGATGTCGATGTCGTAGAATTCTCGTATTTTGGTGCACCCAACCCTGCTCCTAAAGAACAGCTATACACCGAGATTGTCGATGACCTGCGTGGCTCTGATCTATGCATTGGCTCTGGTTCTCAGGTGGCTAGCCAGGAGACTTATGGGACCTTGGGTGCTATCGTAAGGAGTCAAACGGGCAGTCGACAAGTTGGTTTTCTCACGAACCGTCACGTTGCAGTTGATTTGGATTATCCGAATCAGAAGATGTTTCATCCTCTGCCACCGACGCTTGGGCCGGGGGTGTATCTCGAGACGTTCGTCAGGGCAGATGGGGCGTTTATTCCTTTTGCTGATGATTTCGACATGTCGACCGTCACTACATCTGTAAAAGGTGTGGGAGAGGTCGGTGATGTGAAGTTTATCGACTTGCAGTCGCCTATCAGTACCCTCATAGGGAAGCAGGTCGTGAAAGTCGGAAGAAGTTCGGGCTTGACTACCGGAACTGTTTTGGCCTATGCTCTCGAGTACAATGACGAGAAAGGAATATGCTTCTTAACCGATTTTCTCGTCGTCGGTGAGAATCAACAGACTTTTGATCTCGAAGGAGATAGCGGAAGTCTCATCATTTTAAAGGGCGAGAATCGAGAGAGTTTGCAACCAATTGGGATTATATGGGGTGGAACTGCTAACCGAGGTCGGCTTAAGTTAAAAGTAGGCCAACCTCCTGAGAATTGGACAAGTGGGGTCGATCTCGGGCGCCTTCTCAATCTGCTTGAACTTGATCTAATCACAACTGATGACGGGTTTAAAGCAGCAGTGCAAGAACAAAGAACTGTCTCGGCAACAGTTATCGGGTCGATTGTCGGAGACTCCTCTCCTCCCGACACAACGCTGCCAAAGGATAAAAGTGAAGAGAAGTTTGAGCCATTGGGTTTCCAAATCCAGCATATGCCAACAGAAGTAGAACCTTCTTCAGCTAAAGACCGACCACTCCTGGAGACCGAGTTTCATCTCGAAGCTGGGACGAGCACAGCTCCCAGCGTCGAACATCAGTTCATTCCAAGCCTTTTTAGTTGCTCTCCCTCCCTTTCAAACAGCACTCTGGGTCGTGCTGTTTCCCAGAACCTGTCTTCGCTCCGGAGCGACTGCGAAGACATCTCCGTTTCCTTGCACTTGGGTGATCACGAAGCTAAGAGACGACGTTCGGATGCTTCTGTTTCCATGGAAGAGCTGAAATAG

Coding sequence (CDS)

GTGAACTGCATAATGGAGCAGACAAGACACAACAGCAGGATTAACTGCTCTGGTTCGACACCATCCGAGGAATCAGCTCTGGATCTTGAAAGGAATTGCTACGGTCACTCTAATTTACCTTCCTTCAGTCCACCTACACTTCAGCCATTTGCATCAGCTGGACAGCATTGTGAGAGCAATGCAGCTTACTTTTCATGGCCGACGCCCATTCGATTAAGCGTTGCAGCCGAGGAGAGGGCAAACTACTTCGCAAATCTTCAAAAAGGGGTGCTACCAGATATCCTTCGTCCATTACCAAAAGGGCAGCGAGCCACCACGTTACTCGAGCTCATGACAATACGAGCCTTCCATAGCAAGATCCTACGTTGTTACAGTCTCGGAACGGCGATCGGATTCCGTATCCAAAAGGGTGTGCTGACCGACATTCCTGCTATTCTTGTTTTTGTTTCCAGGAAAGTTCATAAGCAATGGCTTAGTCCTATCCAATGCCTGCCCACCGCGTTGGAGGGGCCGGGAGGTGTGTGGTGTGATGTCGATGTCGTAGAATTCTCGTATTTTGGTGCACCCAACCCTGCTCCTAAAGAACAGCTATACACCGAGATTGTCGATGACCTGCGTGGCTCTGATCTATGCATTGGCTCTGGTTCTCAGGTGGCTAGCCAGGAGACTTATGGGACCTTGGGTGCTATCGTAAGGAGTCAAACGGGCAGTCGACAAGTTGGTTTTCTCACGAACCGTCACGTTGCAGTTGATTTGGATTATCCGAATCAGAAGATGTTTCATCCTCTGCCACCGACGCTTGGGCCGGGGGTGTATCTCGAGACGTTCGTCAGGGCAGATGGGGCGTTTATTCCTTTTGCTGATGATTTCGACATGTCGACCGTCACTACATCTGTAAAAGGTGTGGGAGAGGTCGGTGATGTGAAGTTTATCGACTTGCAGTCGCCTATCAGTACCCTCATAGGGAAGCAGGTCGTGAAAGTCGGAAGAAGTTCGGGCTTGACTACCGGAACTGTTTTGGCCTATGCTCTCGAGTACAATGACGAGAAAGGAATATGCTTCTTAACCGATTTTCTCGTCGTCGGTGAGAATCAACAGACTTTTGATCTCGAAGGAGATAGCGGAAGTCTCATCATTTTAAAGGGCGAGAATCGAGAGAGTTTGCAACCAATTGGGATTATATGGGGTGGAACTGCTAACCGAGGTCGGCTTAAGTTAAAAGTAGGCCAACCTCCTGAGAATTGGACAAGTGGGGTCGATCTCGGGCGCCTTCTCAATCTGCTTGAACTTGATCTAATCACAACTGATGACGGGTTTAAAGCAGCAGTGCAAGAACAAAGAACTGTCTCGGCAACAGTTATCGGGTCGATTGTCGGAGACTCCTCTCCTCCCGACACAACGCTGCCAAAGGATAAAAGTGAAGAGAAGTTTGAGCCATTGGGTTTCCAAATCCAGCATATGCCAACAGAAGTAGAACCTTCTTCAGCTAAAGACCGACCACTCCTGGAGACCGAGTTTCATCTCGAAGCTGGGACGAGCACAGCTCCCAGCGTCGAACATCAGTTCATTCCAAGCCTTTTTAGTTGCTCTCCCTCCCTTTCAAACAGCACTCTGGGTCGTGCTGTTTCCCAGAACCTGTCTTCGCTCCGGAGCGACTGCGAAGACATCTCCGTTTCCTTGCACTTGGGTGATCACGAAGCTAAGAGACGACGTTCGGATGCTTCTGTTTCCATGGAAGAGCTGAAATAG

Protein sequence

VNCIMEQTRHNSRINCSGSTPSEESALDLERNCYGHSNLPSFSPPTLQPFASAGQHCESNAAYFSWPTPIRLSVAAEERANYFANLQKGVLPDILRPLPKGQRATTLLELMTIRAFHSKILRCYSLGTAIGFRIQKGVLTDIPAILVFVSRKVHKQWLSPIQCLPTALEGPGGVWCDVDVVEFSYFGAPNPAPKEQLYTEIVDDLRGSDLCIGSGSQVASQETYGTLGAIVRSQTGSRQVGFLTNRHVAVDLDYPNQKMFHPLPPTLGPGVYLETFVRADGAFIPFADDFDMSTVTTSVKGVGEVGDVKFIDLQSPISTLIGKQVVKVGRSSGLTTGTVLAYALEYNDEKGICFLTDFLVVGENQQTFDLEGDSGSLIILKGENRESLQPIGIIWGGTANRGRLKLKVGQPPENWTSGVDLGRLLNLLELDLITTDDGFKAAVQEQRTVSATVIGSIVGDSSPPDTTLPKDKSEEKFEPLGFQIQHMPTEVEPSSAKDRPLLETEFHLEAGTSTAPSVEHQFIPSLFSCSPSLSNSTLGRAVSQNLSSLRSDCEDISVSLHLGDHEAKRRRSDASVSMEELK
Homology
BLAST of Carg14778 vs. NCBI nr
Match: KAG7037486.1 (hypothetical protein SDJN02_01113, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 1168.7 bits (3022), Expect = 0.0e+00
Identity = 582/582 (100.00%), Postives = 582/582 (100.00%), Query Frame = 0

Query: 1   VNCIMEQTRHNSRINCSGSTPSEESALDLERNCYGHSNLPSFSPPTLQPFASAGQHCESN 60
           VNCIMEQTRHNSRINCSGSTPSEESALDLERNCYGHSNLPSFSPPTLQPFASAGQHCESN
Sbjct: 1   VNCIMEQTRHNSRINCSGSTPSEESALDLERNCYGHSNLPSFSPPTLQPFASAGQHCESN 60

Query: 61  AAYFSWPTPIRLSVAAEERANYFANLQKGVLPDILRPLPKGQRATTLLELMTIRAFHSKI 120
           AAYFSWPTPIRLSVAAEERANYFANLQKGVLPDILRPLPKGQRATTLLELMTIRAFHSKI
Sbjct: 61  AAYFSWPTPIRLSVAAEERANYFANLQKGVLPDILRPLPKGQRATTLLELMTIRAFHSKI 120

Query: 121 LRCYSLGTAIGFRIQKGVLTDIPAILVFVSRKVHKQWLSPIQCLPTALEGPGGVWCDVDV 180
           LRCYSLGTAIGFRIQKGVLTDIPAILVFVSRKVHKQWLSPIQCLPTALEGPGGVWCDVDV
Sbjct: 121 LRCYSLGTAIGFRIQKGVLTDIPAILVFVSRKVHKQWLSPIQCLPTALEGPGGVWCDVDV 180

Query: 181 VEFSYFGAPNPAPKEQLYTEIVDDLRGSDLCIGSGSQVASQETYGTLGAIVRSQTGSRQV 240
           VEFSYFGAPNPAPKEQLYTEIVDDLRGSDLCIGSGSQVASQETYGTLGAIVRSQTGSRQV
Sbjct: 181 VEFSYFGAPNPAPKEQLYTEIVDDLRGSDLCIGSGSQVASQETYGTLGAIVRSQTGSRQV 240

Query: 241 GFLTNRHVAVDLDYPNQKMFHPLPPTLGPGVYLETFVRADGAFIPFADDFDMSTVTTSVK 300
           GFLTNRHVAVDLDYPNQKMFHPLPPTLGPGVYLETFVRADGAFIPFADDFDMSTVTTSVK
Sbjct: 241 GFLTNRHVAVDLDYPNQKMFHPLPPTLGPGVYLETFVRADGAFIPFADDFDMSTVTTSVK 300

Query: 301 GVGEVGDVKFIDLQSPISTLIGKQVVKVGRSSGLTTGTVLAYALEYNDEKGICFLTDFLV 360
           GVGEVGDVKFIDLQSPISTLIGKQVVKVGRSSGLTTGTVLAYALEYNDEKGICFLTDFLV
Sbjct: 301 GVGEVGDVKFIDLQSPISTLIGKQVVKVGRSSGLTTGTVLAYALEYNDEKGICFLTDFLV 360

Query: 361 VGENQQTFDLEGDSGSLIILKGENRESLQPIGIIWGGTANRGRLKLKVGQPPENWTSGVD 420
           VGENQQTFDLEGDSGSLIILKGENRESLQPIGIIWGGTANRGRLKLKVGQPPENWTSGVD
Sbjct: 361 VGENQQTFDLEGDSGSLIILKGENRESLQPIGIIWGGTANRGRLKLKVGQPPENWTSGVD 420

Query: 421 LGRLLNLLELDLITTDDGFKAAVQEQRTVSATVIGSIVGDSSPPDTTLPKDKSEEKFEPL 480
           LGRLLNLLELDLITTDDGFKAAVQEQRTVSATVIGSIVGDSSPPDTTLPKDKSEEKFEPL
Sbjct: 421 LGRLLNLLELDLITTDDGFKAAVQEQRTVSATVIGSIVGDSSPPDTTLPKDKSEEKFEPL 480

Query: 481 GFQIQHMPTEVEPSSAKDRPLLETEFHLEAGTSTAPSVEHQFIPSLFSCSPSLSNSTLGR 540
           GFQIQHMPTEVEPSSAKDRPLLETEFHLEAGTSTAPSVEHQFIPSLFSCSPSLSNSTLGR
Sbjct: 481 GFQIQHMPTEVEPSSAKDRPLLETEFHLEAGTSTAPSVEHQFIPSLFSCSPSLSNSTLGR 540

Query: 541 AVSQNLSSLRSDCEDISVSLHLGDHEAKRRRSDASVSMEELK 583
           AVSQNLSSLRSDCEDISVSLHLGDHEAKRRRSDASVSMEELK
Sbjct: 541 AVSQNLSSLRSDCEDISVSLHLGDHEAKRRRSDASVSMEELK 582

BLAST of Carg14778 vs. NCBI nr
Match: KAG6607973.1 (Protein NARROW LEAF 1, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 1155.2 bits (2987), Expect = 0.0e+00
Identity = 582/606 (96.04%), Postives = 582/606 (96.04%), Query Frame = 0

Query: 1   VNCIMEQTRHNSRINCSGSTPSEESALDLERNCYGHSNLPSFSPPTLQPFASAGQHCESN 60
           VNCIMEQTRHNSRINCSGSTPSEESALDLERNCYGHSNLPSFSPPTLQPFASAGQHCESN
Sbjct: 74  VNCIMEQTRHNSRINCSGSTPSEESALDLERNCYGHSNLPSFSPPTLQPFASAGQHCESN 133

Query: 61  AAYFSWPTPIRLSVAAEERANYFANLQKGVLPDILRPLPKGQRATTLLELMTIRAFHSKI 120
           AAYFSWPTPIRLSVAAEERANYFANLQKGVLPDILRPLPKGQRATTLLELMTIRAFHSKI
Sbjct: 134 AAYFSWPTPIRLSVAAEERANYFANLQKGVLPDILRPLPKGQRATTLLELMTIRAFHSKI 193

Query: 121 LRCYSLGTAIGFRIQKGVLTDIPAILVFVSRKVHKQWLSPIQCLPTALEGPGGVWCDVDV 180
           LRCYSLGTAIGFRIQKGVLTDIPAILVFVSRKVHKQWLSPIQCLPTALEGPGGVWCDVDV
Sbjct: 194 LRCYSLGTAIGFRIQKGVLTDIPAILVFVSRKVHKQWLSPIQCLPTALEGPGGVWCDVDV 253

Query: 181 VEFSYFGAPNPAPKEQLYTEIVDDLRGSDLCIGSGSQVASQETYGTLGAIVRSQTGSRQV 240
           VEFSYFGAPNPAPKEQLYTEIVDDLRGSDLCIGSGSQVASQETYGTLGAIVRSQTGSRQV
Sbjct: 254 VEFSYFGAPNPAPKEQLYTEIVDDLRGSDLCIGSGSQVASQETYGTLGAIVRSQTGSRQV 313

Query: 241 GFLTNRHVAVDLDYPNQKMFHPLPPTLGPGVYL------------------------ETF 300
           GFLTNRHVAVDLDYPNQKMFHPLPPTLGPGVYL                        ETF
Sbjct: 314 GFLTNRHVAVDLDYPNQKMFHPLPPTLGPGVYLGAVERATSFITDELWYGIFAGINPETF 373

Query: 301 VRADGAFIPFADDFDMSTVTTSVKGVGEVGDVKFIDLQSPISTLIGKQVVKVGRSSGLTT 360
           VRADGAFIPFADDFDMSTVTTSVKGVGEVGDVKFIDLQSPISTLIGKQVVKVGRSSGLTT
Sbjct: 374 VRADGAFIPFADDFDMSTVTTSVKGVGEVGDVKFIDLQSPISTLIGKQVVKVGRSSGLTT 433

Query: 361 GTVLAYALEYNDEKGICFLTDFLVVGENQQTFDLEGDSGSLIILKGENRESLQPIGIIWG 420
           GTVLAYALEYNDEKGICFLTDFLVVGENQQTFDLEGDSGSLIILKGENRESLQPIGIIWG
Sbjct: 434 GTVLAYALEYNDEKGICFLTDFLVVGENQQTFDLEGDSGSLIILKGENRESLQPIGIIWG 493

Query: 421 GTANRGRLKLKVGQPPENWTSGVDLGRLLNLLELDLITTDDGFKAAVQEQRTVSATVIGS 480
           GTANRGRLKLKVGQPPENWTSGVDLGRLLNLLELDLITTDDGFKAAVQEQRTVSATVIGS
Sbjct: 494 GTANRGRLKLKVGQPPENWTSGVDLGRLLNLLELDLITTDDGFKAAVQEQRTVSATVIGS 553

Query: 481 IVGDSSPPDTTLPKDKSEEKFEPLGFQIQHMPTEVEPSSAKDRPLLETEFHLEAGTSTAP 540
           IVGDSSPPDTTLPKDKSEEKFEPLGFQIQHMPTEVEPSSAKDRPLLETEFHLEAGTSTAP
Sbjct: 554 IVGDSSPPDTTLPKDKSEEKFEPLGFQIQHMPTEVEPSSAKDRPLLETEFHLEAGTSTAP 613

Query: 541 SVEHQFIPSLFSCSPSLSNSTLGRAVSQNLSSLRSDCEDISVSLHLGDHEAKRRRSDASV 583
           SVEHQFIPSLFSCSPSLSNSTLGRAVSQNLSSLRSDCEDISVSLHLGDHEAKRRRSDASV
Sbjct: 614 SVEHQFIPSLFSCSPSLSNSTLGRAVSQNLSSLRSDCEDISVSLHLGDHEAKRRRSDASV 673

BLAST of Carg14778 vs. NCBI nr
Match: XP_022981089.1 (uncharacterized protein LOC111480347 [Cucurbita maxima] >XP_023524995.1 uncharacterized protein LOC111788750 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 1144.0 bits (2958), Expect = 0.0e+00
Identity = 577/602 (95.85%), Postives = 577/602 (95.85%), Query Frame = 0

Query: 5   MEQTRHNSRINCSGSTPSEESALDLERNCYGHSNLPSFSPPTLQPFASAGQHCESNAAYF 64
           MEQTRHNSRINCSGSTPSEESALDLERNCYGHSNLPSFSPPTLQPFASAGQHCESNAAYF
Sbjct: 1   MEQTRHNSRINCSGSTPSEESALDLERNCYGHSNLPSFSPPTLQPFASAGQHCESNAAYF 60

Query: 65  SWPTPIRLSVAAEERANYFANLQKGVLPDILRPLPKGQRATTLLELMTIRAFHSKILRCY 124
           SWPTPIRLSVAAEERANYFANLQKGVLPDIL PLPKGQRATTLLELMTIRAFHSKILRCY
Sbjct: 61  SWPTPIRLSVAAEERANYFANLQKGVLPDILHPLPKGQRATTLLELMTIRAFHSKILRCY 120

Query: 125 SLGTAIGFRIQKGVLTDIPAILVFVSRKVHKQWLSPIQCLPTALEGPGGVWCDVDVVEFS 184
           SLGTAIGFRIQKGVLTDIPAILVFVSRKVHKQWLSPIQCLPTALEGPGGVWCDVDVVEFS
Sbjct: 121 SLGTAIGFRIQKGVLTDIPAILVFVSRKVHKQWLSPIQCLPTALEGPGGVWCDVDVVEFS 180

Query: 185 YFGAPNPAPKEQLYTEIVDDLRGSDLCIGSGSQVASQETYGTLGAIVRSQTGSRQVGFLT 244
           YFGAPNPAPKEQLYTEIVDDLRGSDLCIGSGSQVASQETYGTLGAIVRSQTGSRQVGFLT
Sbjct: 181 YFGAPNPAPKEQLYTEIVDDLRGSDLCIGSGSQVASQETYGTLGAIVRSQTGSRQVGFLT 240

Query: 245 NRHVAVDLDYPNQKMFHPLPPTLGPGVYL------------------------ETFVRAD 304
           NRHVAVDLDYPNQKMFHPLPPTLGPGVYL                        ETFVRAD
Sbjct: 241 NRHVAVDLDYPNQKMFHPLPPTLGPGVYLGAVERATSFITDELWYGIFAGINPETFVRAD 300

Query: 305 GAFIPFADDFDMSTVTTSVKGVGEVGDVKFIDLQSPISTLIGKQVVKVGRSSGLTTGTVL 364
           GAFIPFADDFDMSTVTTSVKGVGEVGDVKFIDLQSPISTLIGKQVVKVGRSSGLTTGTVL
Sbjct: 301 GAFIPFADDFDMSTVTTSVKGVGEVGDVKFIDLQSPISTLIGKQVVKVGRSSGLTTGTVL 360

Query: 365 AYALEYNDEKGICFLTDFLVVGENQQTFDLEGDSGSLIILKGENRESLQPIGIIWGGTAN 424
           AYALEYNDEKGICFLTDFLVVGENQQTFDLEGDSGSLIILKGENRESLQPIGIIWGGTAN
Sbjct: 361 AYALEYNDEKGICFLTDFLVVGENQQTFDLEGDSGSLIILKGENRESLQPIGIIWGGTAN 420

Query: 425 RGRLKLKVGQPPENWTSGVDLGRLLNLLELDLITTDDGFKAAVQEQRTVSATVIGSIVGD 484
           RGRLKLKVGQPPENWTSGVDLGRLLNLLELDLITTDDGFKAAVQEQRTVSATVIGSIVGD
Sbjct: 421 RGRLKLKVGQPPENWTSGVDLGRLLNLLELDLITTDDGFKAAVQEQRTVSATVIGSIVGD 480

Query: 485 SSPPDTTLPKDKSEEKFEPLGFQIQHMPTEVEPSSAKDRPLLETEFHLEAGTSTAPSVEH 544
           SSPPDTTLPKDKSEEKFEPLGFQIQHMPTEVEPSSAKDRPLLETEFHLEAGTSTAPSVEH
Sbjct: 481 SSPPDTTLPKDKSEEKFEPLGFQIQHMPTEVEPSSAKDRPLLETEFHLEAGTSTAPSVEH 540

Query: 545 QFIPSLFSCSPSLSNSTLGRAVSQNLSSLRSDCEDISVSLHLGDHEAKRRRSDASVSMEE 583
           QFIPSLFSCSPSLSNSTLGRAVSQNLSSLRSDCEDISVSLHLGDHEAKRRRSDASVSMEE
Sbjct: 541 QFIPSLFSCSPSLSNSTLGRAVSQNLSSLRSDCEDISVSLHLGDHEAKRRRSDASVSMEE 600

BLAST of Carg14778 vs. NCBI nr
Match: XP_022940289.1 (uncharacterized protein LOC111445958 [Cucurbita moschata])

HSP 1 Score: 1140.9 bits (2950), Expect = 0.0e+00
Identity = 575/602 (95.51%), Postives = 577/602 (95.85%), Query Frame = 0

Query: 5   MEQTRHNSRINCSGSTPSEESALDLERNCYGHSNLPSFSPPTLQPFASAGQHCESNAAYF 64
           MEQTRHNSRINCSGSTPSEESALDLERNCYGHSNLPSFSPPTLQPFASAGQHCESNAAYF
Sbjct: 1   MEQTRHNSRINCSGSTPSEESALDLERNCYGHSNLPSFSPPTLQPFASAGQHCESNAAYF 60

Query: 65  SWPTPIRLSVAAEERANYFANLQKGVLPDILRPLPKGQRATTLLELMTIRAFHSKILRCY 124
           SWPTPIRLSVAAEERANYFANLQKGVLPDIL PLPKGQRATTLLELMTIRAFHSKILRCY
Sbjct: 61  SWPTPIRLSVAAEERANYFANLQKGVLPDILHPLPKGQRATTLLELMTIRAFHSKILRCY 120

Query: 125 SLGTAIGFRIQKGVLTDIPAILVFVSRKVHKQWLSPIQCLPTALEGPGGVWCDVDVVEFS 184
           SLGTAIGFRIQKGVLTDIPAILVFVSRKVHKQWLSPIQCLPTALEGPGGVWCDVDVVEFS
Sbjct: 121 SLGTAIGFRIQKGVLTDIPAILVFVSRKVHKQWLSPIQCLPTALEGPGGVWCDVDVVEFS 180

Query: 185 YFGAPNPAPKEQLYTEIVDDLRGSDLCIGSGSQVASQETYGTLGAIVRSQTGSRQVGFLT 244
           YFGAPNPAPKEQLYTEIVDDLRGSDLCIGSGSQVASQETYGTLGAIVRSQTGSRQVGFLT
Sbjct: 181 YFGAPNPAPKEQLYTEIVDDLRGSDLCIGSGSQVASQETYGTLGAIVRSQTGSRQVGFLT 240

Query: 245 NRHVAVDLDYPNQKMFHPLPPTLGPGVYL------------------------ETFVRAD 304
           NRHVAVDLDYPNQKMFHPLPPTLGPGVYL                        ETFVRAD
Sbjct: 241 NRHVAVDLDYPNQKMFHPLPPTLGPGVYLGAVERATSFITDELWYGIFAGINPETFVRAD 300

Query: 305 GAFIPFADDFDMSTVTTSVKGVGEVGDVKFIDLQSPISTLIGKQVVKVGRSSGLTTGTVL 364
           GAFIPFADDFDMSTVTTSVKGVGEVGDVKFIDLQSPISTLIGKQVVKVGRSSGLTTGTVL
Sbjct: 301 GAFIPFADDFDMSTVTTSVKGVGEVGDVKFIDLQSPISTLIGKQVVKVGRSSGLTTGTVL 360

Query: 365 AYALEYNDEKGICFLTDFLVVGENQQTFDLEGDSGSLIILKGENRESLQPIGIIWGGTAN 424
           AYALEYNDEKGICFLTDFLVVGENQQTFDLEGDSGSLIILKGENRESLQPIGIIWGGTAN
Sbjct: 361 AYALEYNDEKGICFLTDFLVVGENQQTFDLEGDSGSLIILKGENRESLQPIGIIWGGTAN 420

Query: 425 RGRLKLKVGQPPENWTSGVDLGRLLNLLELDLITTDDGFKAAVQEQRTVSATVIGSIVGD 484
           RGRLKLKVGQPPENWTSGVDLGRLLNLLELDLITTDDGFKAAVQEQRTVSATVIGSIVGD
Sbjct: 421 RGRLKLKVGQPPENWTSGVDLGRLLNLLELDLITTDDGFKAAVQEQRTVSATVIGSIVGD 480

Query: 485 SSPPDTTLPKDKSEEKFEPLGFQIQHMPTEVEPSSAKDRPLLETEFHLEAGTSTAPSVEH 544
           SSPPDTTLPKDKSEEKFEPLGFQIQHMPTEVEPSSAKDRPLLETEFHLEAGTSTAP+VEH
Sbjct: 481 SSPPDTTLPKDKSEEKFEPLGFQIQHMPTEVEPSSAKDRPLLETEFHLEAGTSTAPNVEH 540

Query: 545 QFIPSLFSCSPSLSNSTLGRAVSQNLSSLRSDCEDISVSLHLGDHEAKRRRSDASVSMEE 583
           QFIPSLFSCSPSLSNSTLGRAVSQNLSSLRS+CEDISVSLHLGDHEAKRRRSDASVSMEE
Sbjct: 541 QFIPSLFSCSPSLSNSTLGRAVSQNLSSLRSNCEDISVSLHLGDHEAKRRRSDASVSMEE 600

BLAST of Carg14778 vs. NCBI nr
Match: XP_022136083.1 (uncharacterized protein LOC111007860 isoform X1 [Momordica charantia])

HSP 1 Score: 1106.3 bits (2860), Expect = 0.0e+00
Identity = 558/602 (92.69%), Postives = 567/602 (94.19%), Query Frame = 0

Query: 5   MEQTRHNSRINCSGSTPSEESALDLERNCYGHSNLPSFSPPTLQPFASAGQHCESNAAYF 64
           MEQTRHN RINCSGSTPSEESALDLERNC  HSNLPSFSPPTLQPFASAGQHCESNAAYF
Sbjct: 1   MEQTRHNGRINCSGSTPSEESALDLERNCCSHSNLPSFSPPTLQPFASAGQHCESNAAYF 60

Query: 65  SWPTPIRLSVAAEERANYFANLQKGVLPDILRPLPKGQRATTLLELMTIRAFHSKILRCY 124
           SWPTPIRLSVAAEERANYFANLQKGVLPDIL PLPKGQRATTLLELMTIRAFHSKILRCY
Sbjct: 61  SWPTPIRLSVAAEERANYFANLQKGVLPDILHPLPKGQRATTLLELMTIRAFHSKILRCY 120

Query: 125 SLGTAIGFRIQKGVLTDIPAILVFVSRKVHKQWLSPIQCLPTALEGPGGVWCDVDVVEFS 184
           SLGTAIGFRI+KGVLTDIPAILVFVSRKVHKQWLSPIQCLPTALEGPGGVWCDVDVVEFS
Sbjct: 121 SLGTAIGFRIRKGVLTDIPAILVFVSRKVHKQWLSPIQCLPTALEGPGGVWCDVDVVEFS 180

Query: 185 YFGAPNPAPKEQLYTEIVDDLRGSDLCIGSGSQVASQETYGTLGAIVRSQTGSRQVGFLT 244
           YFGAPNPAPKEQLYTEIVDDLRGSDLCIGSGSQVASQETYGTLGAIVRSQTGSRQVGFLT
Sbjct: 181 YFGAPNPAPKEQLYTEIVDDLRGSDLCIGSGSQVASQETYGTLGAIVRSQTGSRQVGFLT 240

Query: 245 NRHVAVDLDYPNQKMFHPLPPTLGPGVYL------------------------ETFVRAD 304
           NRHVAVDLDYPNQKMFHPLPPTLGPGVYL                        ETFVRAD
Sbjct: 241 NRHVAVDLDYPNQKMFHPLPPTLGPGVYLGAVERATSFITDELWYGIFAGINPETFVRAD 300

Query: 305 GAFIPFADDFDMSTVTTSVKGVGEVGDVKFIDLQSPISTLIGKQVVKVGRSSGLTTGTVL 364
           GAFIPFADDF+MSTVTTSVKGVGEVGDVKFIDLQSPISTLIGK+VVKVGRSSGLTTGTVL
Sbjct: 301 GAFIPFADDFEMSTVTTSVKGVGEVGDVKFIDLQSPISTLIGKRVVKVGRSSGLTTGTVL 360

Query: 365 AYALEYNDEKGICFLTDFLVVGENQQTFDLEGDSGSLIILKGENRESLQPIGIIWGGTAN 424
           AYALEYNDEKGICFLTDFLVVGENQQTFDLEGDSGSLIILKGENRESLQPIGIIWGGTAN
Sbjct: 361 AYALEYNDEKGICFLTDFLVVGENQQTFDLEGDSGSLIILKGENRESLQPIGIIWGGTAN 420

Query: 425 RGRLKLKVGQPPENWTSGVDLGRLLNLLELDLITTDDGFKAAVQEQRTVSATVIGSIVGD 484
           RGRLKLKVGQPPENWTSGVDLGRLLNLLELDLIT+D+G KAAVQEQRTVSATVIGSIVGD
Sbjct: 421 RGRLKLKVGQPPENWTSGVDLGRLLNLLELDLITSDEGLKAAVQEQRTVSATVIGSIVGD 480

Query: 485 SSPPDTTLPKDKSEEKFEPLGFQIQHMPTEVEPSSAKDRPLLETEFHLEAGTSTAPSVEH 544
           SSPPDTTLPK+KSEEKFEPLGFQIQHMPTEVEPSSA++RPLLETEFHLEAGTS APSVEH
Sbjct: 481 SSPPDTTLPKEKSEEKFEPLGFQIQHMPTEVEPSSAEERPLLETEFHLEAGTSMAPSVEH 540

Query: 545 QFIPSLFSCSPSLSNSTLGRAVSQNLSSLRSDCEDISVSLHLGDHEAKRRRSDASVSMEE 583
           QFIPSLFSCSPS  NS+L RAVSQNLSSLRSDCEDI VSL LGDHEAKRRRSDASVSMEE
Sbjct: 541 QFIPSLFSCSPSHQNSSLDRAVSQNLSSLRSDCEDICVSLQLGDHEAKRRRSDASVSMEE 600

BLAST of Carg14778 vs. ExPASy Swiss-Prot
Match: B4XT64 (Protein NARROW LEAF 1 OS=Oryza sativa subsp. japonica OX=39947 GN=NAL1 PE=1 SV=1)

HSP 1 Score: 679.5 bits (1752), Expect = 3.4e-194
Identity = 358/593 (60.37%), Postives = 430/593 (72.51%), Query Frame = 0

Query: 11  NSRINCSGSTPSEESALDLERNCYGHSNLPSFSPPTLQPFASAGQHCESNAAYFSWPTPI 70
           + +   SG   SEES+LD++     H + P    P++QP AS   H E++AAYF WPT  
Sbjct: 5   DDKAQLSGLAQSEESSLDVD-----HQSFP--CSPSIQPVASGCTHTENSAAYFLWPTSN 64

Query: 71  RLSVAAEERANYFANLQKGVLPDILRPLPKGQRATTLLELMTIRAFHSKILRCYSLGTAI 130
               AAE RANYF NLQKG+LP     LPKGQ+A +LL+LMTIRAFHSKILR +SLGTA+
Sbjct: 65  LQHCAAEGRANYFGNLQKGLLPRHPGRLPKGQQANSLLDLMTIRAFHSKILRRFSLGTAV 124

Query: 131 GFRIQKGVLTDIPAILVFVSRKVHKQWLSPIQCLPTALEGPGGVWCDVDVVEFSYFGAPN 190
           GFRI+KG LTDIPAILVFV+RKVHK+WL+P QCLP  LEGPGGVWCDVDVVEFSY+GAP 
Sbjct: 125 GFRIRKGDLTDIPAILVFVARKVHKKWLNPAQCLPAILEGPGGVWCDVDVVEFSYYGAPA 184

Query: 191 PAPKEQLYTEIVDDLRGSDLCIGSGSQVASQETYGTLGAIVRSQTGSRQVGFLTNRHVAV 250
             PKEQ+++E+VD L GSD CIGSGSQVAS ET+GTLGAIV+ +TG++QVGFLTN HVAV
Sbjct: 185 QTPKEQMFSELVDKLCGSDECIGSGSQVASHETFGTLGAIVKRRTGNKQVGFLTNHHVAV 244

Query: 251 DLDYPNQKMFHPLPPTLGPGVYL------------------------ETFVRADGAFIPF 310
           DLDYPNQKMFHPLPP LGPGVYL                        ETFVRADGAFIPF
Sbjct: 245 DLDYPNQKMFHPLPPNLGPGVYLGAVERATSFITDDVWYGIYAGTNPETFVRADGAFIPF 304

Query: 311 ADDFDMSTVTTSVKGVGEVGDVKFIDLQSPISTLIGKQVVKVGRSSGLTTGTVLAYALEY 370
           ADDFD+STVTT V+GVG++GDVK IDLQ P+++LIG+QV KVGRSSG TTGTV+AYALEY
Sbjct: 305 ADDFDISTVTTVVRGVGDIGDVKVIDLQCPLNSLIGRQVCKVGRSSGHTTGTVMAYALEY 364

Query: 371 NDEKGICFLTDFLVVGENQQTFDLEGDSGSLIILKGENRESLQPIGIIWGGTANRGRLKL 430
           NDEKGICF TD LVVGEN+QTFDLEGDSGSLIIL  ++ E  +PIGIIWGGTANRGRLKL
Sbjct: 365 NDEKGICFFTDILVVGENRQTFDLEGDSGSLIILTSQDGEKPRPIGIIWGGTANRGRLKL 424

Query: 431 KVGQPPENWTSGVDLGRLLNLLELDLITTDDGFKAAVQEQRTVSATVIGSIVGDSSPPDT 490
                PENWTSGVDLGRLL+ LELD+I T++  + AVQ+QR      + S VG+SS    
Sbjct: 425 TSDHGPENWTSGVDLGRLLDRLELDIIITNESLQDAVQQQRFALVAAVTSAVGESSGVPV 484

Query: 491 TLPKDKSEEKFEPLGFQIQHMPTEVEPSSAKDRPLLETEFHLEAGTSTAPSVEHQFIPSL 550
            +P++K EE FEPLG QIQ +P     +S  +          EA  +     EHQFI + 
Sbjct: 485 AIPEEKIEEIFEPLGIQIQQLPRHDVAASGTEGE--------EASNTVVNVEEHQFISNF 544

Query: 551 FSCSPSLSNSTLGRAVSQNLSSLRSDCEDISVSLHLGDHEAKRRRSDASVSME 580
              SP   +    R+++ NL++     E++++SLHLGD E KR RSD+  S++
Sbjct: 545 VGMSPVRDDQDAPRSIT-NLNNPSE--EELAMSLHLGDREPKRLRSDSGSSLD 579

BLAST of Carg14778 vs. ExPASy TrEMBL
Match: A0A6J1J109 (uncharacterized protein LOC111480347 OS=Cucurbita maxima OX=3661 GN=LOC111480347 PE=4 SV=1)

HSP 1 Score: 1144.0 bits (2958), Expect = 0.0e+00
Identity = 577/602 (95.85%), Postives = 577/602 (95.85%), Query Frame = 0

Query: 5   MEQTRHNSRINCSGSTPSEESALDLERNCYGHSNLPSFSPPTLQPFASAGQHCESNAAYF 64
           MEQTRHNSRINCSGSTPSEESALDLERNCYGHSNLPSFSPPTLQPFASAGQHCESNAAYF
Sbjct: 1   MEQTRHNSRINCSGSTPSEESALDLERNCYGHSNLPSFSPPTLQPFASAGQHCESNAAYF 60

Query: 65  SWPTPIRLSVAAEERANYFANLQKGVLPDILRPLPKGQRATTLLELMTIRAFHSKILRCY 124
           SWPTPIRLSVAAEERANYFANLQKGVLPDIL PLPKGQRATTLLELMTIRAFHSKILRCY
Sbjct: 61  SWPTPIRLSVAAEERANYFANLQKGVLPDILHPLPKGQRATTLLELMTIRAFHSKILRCY 120

Query: 125 SLGTAIGFRIQKGVLTDIPAILVFVSRKVHKQWLSPIQCLPTALEGPGGVWCDVDVVEFS 184
           SLGTAIGFRIQKGVLTDIPAILVFVSRKVHKQWLSPIQCLPTALEGPGGVWCDVDVVEFS
Sbjct: 121 SLGTAIGFRIQKGVLTDIPAILVFVSRKVHKQWLSPIQCLPTALEGPGGVWCDVDVVEFS 180

Query: 185 YFGAPNPAPKEQLYTEIVDDLRGSDLCIGSGSQVASQETYGTLGAIVRSQTGSRQVGFLT 244
           YFGAPNPAPKEQLYTEIVDDLRGSDLCIGSGSQVASQETYGTLGAIVRSQTGSRQVGFLT
Sbjct: 181 YFGAPNPAPKEQLYTEIVDDLRGSDLCIGSGSQVASQETYGTLGAIVRSQTGSRQVGFLT 240

Query: 245 NRHVAVDLDYPNQKMFHPLPPTLGPGVYL------------------------ETFVRAD 304
           NRHVAVDLDYPNQKMFHPLPPTLGPGVYL                        ETFVRAD
Sbjct: 241 NRHVAVDLDYPNQKMFHPLPPTLGPGVYLGAVERATSFITDELWYGIFAGINPETFVRAD 300

Query: 305 GAFIPFADDFDMSTVTTSVKGVGEVGDVKFIDLQSPISTLIGKQVVKVGRSSGLTTGTVL 364
           GAFIPFADDFDMSTVTTSVKGVGEVGDVKFIDLQSPISTLIGKQVVKVGRSSGLTTGTVL
Sbjct: 301 GAFIPFADDFDMSTVTTSVKGVGEVGDVKFIDLQSPISTLIGKQVVKVGRSSGLTTGTVL 360

Query: 365 AYALEYNDEKGICFLTDFLVVGENQQTFDLEGDSGSLIILKGENRESLQPIGIIWGGTAN 424
           AYALEYNDEKGICFLTDFLVVGENQQTFDLEGDSGSLIILKGENRESLQPIGIIWGGTAN
Sbjct: 361 AYALEYNDEKGICFLTDFLVVGENQQTFDLEGDSGSLIILKGENRESLQPIGIIWGGTAN 420

Query: 425 RGRLKLKVGQPPENWTSGVDLGRLLNLLELDLITTDDGFKAAVQEQRTVSATVIGSIVGD 484
           RGRLKLKVGQPPENWTSGVDLGRLLNLLELDLITTDDGFKAAVQEQRTVSATVIGSIVGD
Sbjct: 421 RGRLKLKVGQPPENWTSGVDLGRLLNLLELDLITTDDGFKAAVQEQRTVSATVIGSIVGD 480

Query: 485 SSPPDTTLPKDKSEEKFEPLGFQIQHMPTEVEPSSAKDRPLLETEFHLEAGTSTAPSVEH 544
           SSPPDTTLPKDKSEEKFEPLGFQIQHMPTEVEPSSAKDRPLLETEFHLEAGTSTAPSVEH
Sbjct: 481 SSPPDTTLPKDKSEEKFEPLGFQIQHMPTEVEPSSAKDRPLLETEFHLEAGTSTAPSVEH 540

Query: 545 QFIPSLFSCSPSLSNSTLGRAVSQNLSSLRSDCEDISVSLHLGDHEAKRRRSDASVSMEE 583
           QFIPSLFSCSPSLSNSTLGRAVSQNLSSLRSDCEDISVSLHLGDHEAKRRRSDASVSMEE
Sbjct: 541 QFIPSLFSCSPSLSNSTLGRAVSQNLSSLRSDCEDISVSLHLGDHEAKRRRSDASVSMEE 600

BLAST of Carg14778 vs. ExPASy TrEMBL
Match: A0A6J1FJM9 (uncharacterized protein LOC111445958 OS=Cucurbita moschata OX=3662 GN=LOC111445958 PE=4 SV=1)

HSP 1 Score: 1140.9 bits (2950), Expect = 0.0e+00
Identity = 575/602 (95.51%), Postives = 577/602 (95.85%), Query Frame = 0

Query: 5   MEQTRHNSRINCSGSTPSEESALDLERNCYGHSNLPSFSPPTLQPFASAGQHCESNAAYF 64
           MEQTRHNSRINCSGSTPSEESALDLERNCYGHSNLPSFSPPTLQPFASAGQHCESNAAYF
Sbjct: 1   MEQTRHNSRINCSGSTPSEESALDLERNCYGHSNLPSFSPPTLQPFASAGQHCESNAAYF 60

Query: 65  SWPTPIRLSVAAEERANYFANLQKGVLPDILRPLPKGQRATTLLELMTIRAFHSKILRCY 124
           SWPTPIRLSVAAEERANYFANLQKGVLPDIL PLPKGQRATTLLELMTIRAFHSKILRCY
Sbjct: 61  SWPTPIRLSVAAEERANYFANLQKGVLPDILHPLPKGQRATTLLELMTIRAFHSKILRCY 120

Query: 125 SLGTAIGFRIQKGVLTDIPAILVFVSRKVHKQWLSPIQCLPTALEGPGGVWCDVDVVEFS 184
           SLGTAIGFRIQKGVLTDIPAILVFVSRKVHKQWLSPIQCLPTALEGPGGVWCDVDVVEFS
Sbjct: 121 SLGTAIGFRIQKGVLTDIPAILVFVSRKVHKQWLSPIQCLPTALEGPGGVWCDVDVVEFS 180

Query: 185 YFGAPNPAPKEQLYTEIVDDLRGSDLCIGSGSQVASQETYGTLGAIVRSQTGSRQVGFLT 244
           YFGAPNPAPKEQLYTEIVDDLRGSDLCIGSGSQVASQETYGTLGAIVRSQTGSRQVGFLT
Sbjct: 181 YFGAPNPAPKEQLYTEIVDDLRGSDLCIGSGSQVASQETYGTLGAIVRSQTGSRQVGFLT 240

Query: 245 NRHVAVDLDYPNQKMFHPLPPTLGPGVYL------------------------ETFVRAD 304
           NRHVAVDLDYPNQKMFHPLPPTLGPGVYL                        ETFVRAD
Sbjct: 241 NRHVAVDLDYPNQKMFHPLPPTLGPGVYLGAVERATSFITDELWYGIFAGINPETFVRAD 300

Query: 305 GAFIPFADDFDMSTVTTSVKGVGEVGDVKFIDLQSPISTLIGKQVVKVGRSSGLTTGTVL 364
           GAFIPFADDFDMSTVTTSVKGVGEVGDVKFIDLQSPISTLIGKQVVKVGRSSGLTTGTVL
Sbjct: 301 GAFIPFADDFDMSTVTTSVKGVGEVGDVKFIDLQSPISTLIGKQVVKVGRSSGLTTGTVL 360

Query: 365 AYALEYNDEKGICFLTDFLVVGENQQTFDLEGDSGSLIILKGENRESLQPIGIIWGGTAN 424
           AYALEYNDEKGICFLTDFLVVGENQQTFDLEGDSGSLIILKGENRESLQPIGIIWGGTAN
Sbjct: 361 AYALEYNDEKGICFLTDFLVVGENQQTFDLEGDSGSLIILKGENRESLQPIGIIWGGTAN 420

Query: 425 RGRLKLKVGQPPENWTSGVDLGRLLNLLELDLITTDDGFKAAVQEQRTVSATVIGSIVGD 484
           RGRLKLKVGQPPENWTSGVDLGRLLNLLELDLITTDDGFKAAVQEQRTVSATVIGSIVGD
Sbjct: 421 RGRLKLKVGQPPENWTSGVDLGRLLNLLELDLITTDDGFKAAVQEQRTVSATVIGSIVGD 480

Query: 485 SSPPDTTLPKDKSEEKFEPLGFQIQHMPTEVEPSSAKDRPLLETEFHLEAGTSTAPSVEH 544
           SSPPDTTLPKDKSEEKFEPLGFQIQHMPTEVEPSSAKDRPLLETEFHLEAGTSTAP+VEH
Sbjct: 481 SSPPDTTLPKDKSEEKFEPLGFQIQHMPTEVEPSSAKDRPLLETEFHLEAGTSTAPNVEH 540

Query: 545 QFIPSLFSCSPSLSNSTLGRAVSQNLSSLRSDCEDISVSLHLGDHEAKRRRSDASVSMEE 583
           QFIPSLFSCSPSLSNSTLGRAVSQNLSSLRS+CEDISVSLHLGDHEAKRRRSDASVSMEE
Sbjct: 541 QFIPSLFSCSPSLSNSTLGRAVSQNLSSLRSNCEDISVSLHLGDHEAKRRRSDASVSMEE 600

BLAST of Carg14778 vs. ExPASy TrEMBL
Match: A0A6J1C6M7 (uncharacterized protein LOC111007860 isoform X1 OS=Momordica charantia OX=3673 GN=LOC111007860 PE=4 SV=1)

HSP 1 Score: 1106.3 bits (2860), Expect = 0.0e+00
Identity = 558/602 (92.69%), Postives = 567/602 (94.19%), Query Frame = 0

Query: 5   MEQTRHNSRINCSGSTPSEESALDLERNCYGHSNLPSFSPPTLQPFASAGQHCESNAAYF 64
           MEQTRHN RINCSGSTPSEESALDLERNC  HSNLPSFSPPTLQPFASAGQHCESNAAYF
Sbjct: 1   MEQTRHNGRINCSGSTPSEESALDLERNCCSHSNLPSFSPPTLQPFASAGQHCESNAAYF 60

Query: 65  SWPTPIRLSVAAEERANYFANLQKGVLPDILRPLPKGQRATTLLELMTIRAFHSKILRCY 124
           SWPTPIRLSVAAEERANYFANLQKGVLPDIL PLPKGQRATTLLELMTIRAFHSKILRCY
Sbjct: 61  SWPTPIRLSVAAEERANYFANLQKGVLPDILHPLPKGQRATTLLELMTIRAFHSKILRCY 120

Query: 125 SLGTAIGFRIQKGVLTDIPAILVFVSRKVHKQWLSPIQCLPTALEGPGGVWCDVDVVEFS 184
           SLGTAIGFRI+KGVLTDIPAILVFVSRKVHKQWLSPIQCLPTALEGPGGVWCDVDVVEFS
Sbjct: 121 SLGTAIGFRIRKGVLTDIPAILVFVSRKVHKQWLSPIQCLPTALEGPGGVWCDVDVVEFS 180

Query: 185 YFGAPNPAPKEQLYTEIVDDLRGSDLCIGSGSQVASQETYGTLGAIVRSQTGSRQVGFLT 244
           YFGAPNPAPKEQLYTEIVDDLRGSDLCIGSGSQVASQETYGTLGAIVRSQTGSRQVGFLT
Sbjct: 181 YFGAPNPAPKEQLYTEIVDDLRGSDLCIGSGSQVASQETYGTLGAIVRSQTGSRQVGFLT 240

Query: 245 NRHVAVDLDYPNQKMFHPLPPTLGPGVYL------------------------ETFVRAD 304
           NRHVAVDLDYPNQKMFHPLPPTLGPGVYL                        ETFVRAD
Sbjct: 241 NRHVAVDLDYPNQKMFHPLPPTLGPGVYLGAVERATSFITDELWYGIFAGINPETFVRAD 300

Query: 305 GAFIPFADDFDMSTVTTSVKGVGEVGDVKFIDLQSPISTLIGKQVVKVGRSSGLTTGTVL 364
           GAFIPFADDF+MSTVTTSVKGVGEVGDVKFIDLQSPISTLIGK+VVKVGRSSGLTTGTVL
Sbjct: 301 GAFIPFADDFEMSTVTTSVKGVGEVGDVKFIDLQSPISTLIGKRVVKVGRSSGLTTGTVL 360

Query: 365 AYALEYNDEKGICFLTDFLVVGENQQTFDLEGDSGSLIILKGENRESLQPIGIIWGGTAN 424
           AYALEYNDEKGICFLTDFLVVGENQQTFDLEGDSGSLIILKGENRESLQPIGIIWGGTAN
Sbjct: 361 AYALEYNDEKGICFLTDFLVVGENQQTFDLEGDSGSLIILKGENRESLQPIGIIWGGTAN 420

Query: 425 RGRLKLKVGQPPENWTSGVDLGRLLNLLELDLITTDDGFKAAVQEQRTVSATVIGSIVGD 484
           RGRLKLKVGQPPENWTSGVDLGRLLNLLELDLIT+D+G KAAVQEQRTVSATVIGSIVGD
Sbjct: 421 RGRLKLKVGQPPENWTSGVDLGRLLNLLELDLITSDEGLKAAVQEQRTVSATVIGSIVGD 480

Query: 485 SSPPDTTLPKDKSEEKFEPLGFQIQHMPTEVEPSSAKDRPLLETEFHLEAGTSTAPSVEH 544
           SSPPDTTLPK+KSEEKFEPLGFQIQHMPTEVEPSSA++RPLLETEFHLEAGTS APSVEH
Sbjct: 481 SSPPDTTLPKEKSEEKFEPLGFQIQHMPTEVEPSSAEERPLLETEFHLEAGTSMAPSVEH 540

Query: 545 QFIPSLFSCSPSLSNSTLGRAVSQNLSSLRSDCEDISVSLHLGDHEAKRRRSDASVSMEE 583
           QFIPSLFSCSPS  NS+L RAVSQNLSSLRSDCEDI VSL LGDHEAKRRRSDASVSMEE
Sbjct: 541 QFIPSLFSCSPSHQNSSLDRAVSQNLSSLRSDCEDICVSLQLGDHEAKRRRSDASVSMEE 600

BLAST of Carg14778 vs. ExPASy TrEMBL
Match: A0A6J1C3A8 (uncharacterized protein LOC111007860 isoform X2 OS=Momordica charantia OX=3673 GN=LOC111007860 PE=4 SV=1)

HSP 1 Score: 1100.1 bits (2844), Expect = 0.0e+00
Identity = 557/602 (92.52%), Postives = 566/602 (94.02%), Query Frame = 0

Query: 5   MEQTRHNSRINCSGSTPSEESALDLERNCYGHSNLPSFSPPTLQPFASAGQHCESNAAYF 64
           MEQTRHN RINCSGSTPSEESALDLERNC  HSNLPSFSPPTLQPFASAGQHCESNAAYF
Sbjct: 1   MEQTRHNGRINCSGSTPSEESALDLERNCCSHSNLPSFSPPTLQPFASAGQHCESNAAYF 60

Query: 65  SWPTPIRLSVAAEERANYFANLQKGVLPDILRPLPKGQRATTLLELMTIRAFHSKILRCY 124
           SWPTPIRLSVAAEERANYFANLQKGVLPDIL PLPKGQRATTLLELMTIRAFHSKILRCY
Sbjct: 61  SWPTPIRLSVAAEERANYFANLQKGVLPDILHPLPKGQRATTLLELMTIRAFHSKILRCY 120

Query: 125 SLGTAIGFRIQKGVLTDIPAILVFVSRKVHKQWLSPIQCLPTALEGPGGVWCDVDVVEFS 184
           SLGTAIGFRI+KGVLTDIPAILVFVSRKVHKQWLSPIQCLPTALEGPGGVWCDVDVVEFS
Sbjct: 121 SLGTAIGFRIRKGVLTDIPAILVFVSRKVHKQWLSPIQCLPTALEGPGGVWCDVDVVEFS 180

Query: 185 YFGAPNPAPKEQLYTEIVDDLRGSDLCIGSGSQVASQETYGTLGAIVRSQTGSRQVGFLT 244
           YFGAPNPAPKEQLYTEIVDDLRGSDLCIGSGSQVASQETYGTLGAIVRSQTGSRQVGFLT
Sbjct: 181 YFGAPNPAPKEQLYTEIVDDLRGSDLCIGSGSQVASQETYGTLGAIVRSQTGSRQVGFLT 240

Query: 245 NRHVAVDLDYPNQKMFHPLPPTLGPGVYL------------------------ETFVRAD 304
           NRHVAVDLDYPNQKMFHPLPPTLGPGVYL                        ETFVRAD
Sbjct: 241 NRHVAVDLDYPNQKMFHPLPPTLGPGVYLGAVERATSFITDELWYGIFAGINPETFVRAD 300

Query: 305 GAFIPFADDFDMSTVTTSVKGVGEVGDVKFIDLQSPISTLIGKQVVKVGRSSGLTTGTVL 364
           GAFIPFADDF+MSTVTTSVKGVGEVGDVKFIDLQSPISTLIGK+VVKVGRSSGLTTGTVL
Sbjct: 301 GAFIPFADDFEMSTVTTSVKGVGEVGDVKFIDLQSPISTLIGKRVVKVGRSSGLTTGTVL 360

Query: 365 AYALEYNDEKGICFLTDFLVVGENQQTFDLEGDSGSLIILKGENRESLQPIGIIWGGTAN 424
           AYALEYNDEKGICFLTDFLVVGENQQTFDLEGDSGSLIILKGENRESLQPIGIIWGGTAN
Sbjct: 361 AYALEYNDEKGICFLTDFLVVGENQQTFDLEGDSGSLIILKGENRESLQPIGIIWGGTAN 420

Query: 425 RGRLKLKVGQPPENWTSGVDLGRLLNLLELDLITTDDGFKAAVQEQRTVSATVIGSIVGD 484
           RGRLKLKVGQPPENWTSGVDLGRLLNLLELDLIT+D+G K AVQEQRTVSATVIGSIVGD
Sbjct: 421 RGRLKLKVGQPPENWTSGVDLGRLLNLLELDLITSDEGLK-AVQEQRTVSATVIGSIVGD 480

Query: 485 SSPPDTTLPKDKSEEKFEPLGFQIQHMPTEVEPSSAKDRPLLETEFHLEAGTSTAPSVEH 544
           SSPPDTTLPK+KSEEKFEPLGFQIQHMPTEVEPSSA++RPLLETEFHLEAGTS APSVEH
Sbjct: 481 SSPPDTTLPKEKSEEKFEPLGFQIQHMPTEVEPSSAEERPLLETEFHLEAGTSMAPSVEH 540

Query: 545 QFIPSLFSCSPSLSNSTLGRAVSQNLSSLRSDCEDISVSLHLGDHEAKRRRSDASVSMEE 583
           QFIPSLFSCSPS  NS+L RAVSQNLSSLRSDCEDI VSL LGDHEAKRRRSDASVSMEE
Sbjct: 541 QFIPSLFSCSPSHQNSSLDRAVSQNLSSLRSDCEDICVSLQLGDHEAKRRRSDASVSMEE 600

BLAST of Carg14778 vs. ExPASy TrEMBL
Match: A0A6J1F9R8 (uncharacterized protein LOC111443385 OS=Cucurbita moschata OX=3662 GN=LOC111443385 PE=4 SV=1)

HSP 1 Score: 1067.8 bits (2760), Expect = 1.6e-308
Identity = 542/603 (89.88%), Postives = 558/603 (92.54%), Query Frame = 0

Query: 5   MEQTRHNSRINCSGSTPSEESALDLERNCYGHSNLPSFSPPTLQPFASAGQHCESNAAYF 64
           MEQTR ++RINCSGSTPSEESAL+LERN   HSNLPSFS PTLQPFASAGQHCESNAAYF
Sbjct: 1   MEQTRRHNRINCSGSTPSEESALNLERNGCSHSNLPSFSSPTLQPFASAGQHCESNAAYF 60

Query: 65  SWPTPIRLSVAAEERANYFANLQKGVLPDILRPLPKGQRATTLLELMTIRAFHSKILRCY 124
           SWPTPIR+SV  EERANYFANLQKGVLPDIL PLPKGQRA TLLELMTIRAFHSKILRCY
Sbjct: 61  SWPTPIRISVGTEERANYFANLQKGVLPDILHPLPKGQRANTLLELMTIRAFHSKILRCY 120

Query: 125 SLGTAIGFRIQKGVLTDIPAILVFVSRKVHKQWLSPIQCLPTALEGPGGVWCDVDVVEFS 184
           SLGTAIGFRI+KGVLTDIPAILVFVSRKVHKQWLSPIQCLPTALEGPGGVWCDVDVVEFS
Sbjct: 121 SLGTAIGFRIRKGVLTDIPAILVFVSRKVHKQWLSPIQCLPTALEGPGGVWCDVDVVEFS 180

Query: 185 YFGAPNPAPKEQLYTEIVDDLRGSDLCIGSGSQVASQETYGTLGAIVRSQTGSRQVGFLT 244
           YFGAPNPAPKEQLYTEIVDDLRGSD CIGSGSQVASQETYGTLGAIVRSQTGSRQVGFLT
Sbjct: 181 YFGAPNPAPKEQLYTEIVDDLRGSDPCIGSGSQVASQETYGTLGAIVRSQTGSRQVGFLT 240

Query: 245 NRHVAVDLDYPNQKMFHPLPPTLGPGVYL------------------------ETFVRAD 304
           NRHVAVDLDYPNQKMFHPLPPTLGPGVYL                        ETFVRAD
Sbjct: 241 NRHVAVDLDYPNQKMFHPLPPTLGPGVYLGAVERATSFITDELWYGIFAGINPETFVRAD 300

Query: 305 GAFIPFADDFDMSTVTTSVKGVGEVGDVKFIDLQSPISTLIGKQVVKVGRSSGLTTGTVL 364
           G+FIPFADDFDMSTVTTSVKGVGE+GDVKFIDLQSPISTLIGKQVVKVGRSSGLTTGTVL
Sbjct: 301 GSFIPFADDFDMSTVTTSVKGVGEIGDVKFIDLQSPISTLIGKQVVKVGRSSGLTTGTVL 360

Query: 365 AYALEYNDEKGICFLTDFLVVGENQQTFDLEGDSGSLIILKGENRESLQPIGIIWGGTAN 424
           AYALEYNDEKGICFLTDFLVVGENQQTFDLEGDSGSLIILKGENRESL+PIGIIWGGTAN
Sbjct: 361 AYALEYNDEKGICFLTDFLVVGENQQTFDLEGDSGSLIILKGENRESLKPIGIIWGGTAN 420

Query: 425 RGRLKLKVGQPPENWTSGVDLGRLLNLLELDLITTDDGFKAAVQEQRTVSATVIGSIVGD 484
           RGRLKLKVGQPPENWTSGVDLGRLLNLLELDLIT+D+G KAAVQEQRTVSATVIGSIVGD
Sbjct: 421 RGRLKLKVGQPPENWTSGVDLGRLLNLLELDLITSDEGLKAAVQEQRTVSATVIGSIVGD 480

Query: 485 SSPPDTTLPKDKSEEKFEPLGFQIQHMPTEVEPSSAKDRP-LLETEFHLEAGTSTAPSVE 544
           SSPPDTTLP++KSEEKFEPLGFQIQHM TEVEPSSAKD+  LLETEFHLEAGTSTAPSVE
Sbjct: 481 SSPPDTTLPREKSEEKFEPLGFQIQHMRTEVEPSSAKDQSLLLETEFHLEAGTSTAPSVE 540

Query: 545 HQFIPSLFSCSPSLSNSTLGRAVSQNLSSLRSDCEDISVSLHLGDHEAKRRRSDASVSME 583
           HQFIPSLFSCSPS  NS+L RAVSQNLSSLR+DCEDI VSL LGDHEAKR+R D SVSME
Sbjct: 541 HQFIPSLFSCSPSHQNSSLVRAVSQNLSSLRNDCEDICVSLQLGDHEAKRQRLDGSVSME 600

BLAST of Carg14778 vs. TAIR 10
Match: AT5G45030.1 (Trypsin family protein )

HSP 1 Score: 701.8 bits (1810), Expect = 4.5e-202
Identity = 382/611 (62.52%), Postives = 457/611 (74.80%), Query Frame = 0

Query: 5   MEQTRHNSRINCSGSTPSEES-ALDLERNCYGHSNLPSFSPPTLQPFASAGQHCESN--A 64
           ME  R + R + S S+ S ES ALDL++N Y H  L S SP  LQPF S  QH E++  A
Sbjct: 1   MEGKRLDLRFHHSTSSQSVESAALDLDKNVYNHIKLASSSP--LQPFPSGAQHPETSAAA 60

Query: 65  AYFSWPTPIRLSVAAEERANYFANLQKGVLPDILRPLPKGQRATTLLELMTIRAFHSKIL 124
           AYFSWPT  RL+ +AE+RANYFANLQKGVLP+    LP G++ATTLLELM IRAFHSK L
Sbjct: 61  AYFSWPTSSRLNDSAEDRANYFANLQKGVLPESFDGLPTGKKATTLLELMMIRAFHSKNL 120

Query: 125 RCYSLGTAIGFRIQKGVLTDIPAILVFVSRKVHKQWLSPIQCLPTALEGPGGVWCDVDVV 184
           R +SLGTAIGFRI++GVLT+I AILVFV+RKVHKQWL+P+QCLPTALEGPGGVWCDVDVV
Sbjct: 121 RRFSLGTAIGFRIRRGVLTNIAAILVFVARKVHKQWLNPLQCLPTALEGPGGVWCDVDVV 180

Query: 185 EFSYFGAPNPAPKEQLYTEIVDDLRGSDLCIGSGSQVASQETYGTLGAIVRSQTGSRQVG 244
           EF Y+GAP   PKEQ+YTE+VDDLRGS   IGSGSQVASQETYGTLGAIV+S+TG RQVG
Sbjct: 181 EFQYYGAPAQTPKEQVYTELVDDLRGSGSSIGSGSQVASQETYGTLGAIVKSKTGIRQVG 240

Query: 245 FLTNRHVAVDLDYPNQKMFHPLPPTLGPGVYL------------------------ETFV 304
           FLTNRHVAVDLDYP+QKMFHPLPP+LGPGVYL                        ETFV
Sbjct: 241 FLTNRHVAVDLDYPSQKMFHPLPPSLGPGVYLGAVERATSFITDDLWYGIFAGTNPETFV 300

Query: 305 RADGAFIPFADDFDMSTVTTSVKGVGEVGDVKFIDLQSPISTLIGKQVVKVGRSSGLTTG 364
           RADGAFIPFA+DF+ + VTT+VKG+GE+GD+   DLQSP+++LIG++VVKVGRSSGLTTG
Sbjct: 301 RADGAFIPFAEDFNTNNVTTTVKGIGEIGDIHATDLQSPVNSLIGRKVVKVGRSSGLTTG 360

Query: 365 TVLAYALEYNDEKGICFLTDFLVVGENQQTFDLEGDSGSLIILKG--ENRESLQPIGIIW 424
           T++AYALEYNDEKGICFLTDFLVVGENQQTFDLEGDSGSLI+L    E  E  +P+GIIW
Sbjct: 361 TIMAYALEYNDEKGICFLTDFLVVGENQQTFDLEGDSGSLILLAAGDEKNEKPRPVGIIW 420

Query: 425 GGTANRGRLKLKVGQPPENWTSGVDLGRLLNLLELDLITTDDGFKAAVQEQRT-VSATVI 484
           GGTANRGRLKLKVG+ PENWTSGVDLGR+LNLLELDLIT+++G +AAV EQR  +    +
Sbjct: 421 GGTANRGRLKLKVGEQPENWTSGVDLGRVLNLLELDLITSNEGLQAAVLEQRNGIMCAAV 480

Query: 485 GSIVGDSSPPDTTLPKDKSEEKFEPLGFQIQHMPTEVEPSSAKDRPLLETEFHLEAGTST 544
            S V +SSP    + + K+ E FEP+   +Q +   +E  ++   P  + E  LE   S 
Sbjct: 481 DSTVVESSPGVCNISRCKTGENFEPINLNVQQV--LIEDDNSNIHPEFQIEDVLE---SV 540

Query: 545 APSVEHQFIPSLFSCSPSLSNSTLG--RAVSQNLSSLR--SDCEDISVSLHLGDHEA-KR 581
           A   EHQFIPS  +   +L     G     S+NLSSL+  S  ++I  SL LG+ +  KR
Sbjct: 541 AVIEEHQFIPSSSNNGSALHQKPNGPENLESKNLSSLKTSSSGDEIGFSLQLGESDTKKR 600

BLAST of Carg14778 vs. TAIR 10
Match: AT5G45030.2 (Trypsin family protein )

HSP 1 Score: 701.8 bits (1810), Expect = 4.5e-202
Identity = 382/611 (62.52%), Postives = 457/611 (74.80%), Query Frame = 0

Query: 5   MEQTRHNSRINCSGSTPSEES-ALDLERNCYGHSNLPSFSPPTLQPFASAGQHCESN--A 64
           ME  R + R + S S+ S ES ALDL++N Y H  L S SP  LQPF S  QH E++  A
Sbjct: 1   MEGKRLDLRFHHSTSSQSVESAALDLDKNVYNHIKLASSSP--LQPFPSGAQHPETSAAA 60

Query: 65  AYFSWPTPIRLSVAAEERANYFANLQKGVLPDILRPLPKGQRATTLLELMTIRAFHSKIL 124
           AYFSWPT  RL+ +AE+RANYFANLQKGVLP+    LP G++ATTLLELM IRAFHSK L
Sbjct: 61  AYFSWPTSSRLNDSAEDRANYFANLQKGVLPESFDGLPTGKKATTLLELMMIRAFHSKNL 120

Query: 125 RCYSLGTAIGFRIQKGVLTDIPAILVFVSRKVHKQWLSPIQCLPTALEGPGGVWCDVDVV 184
           R +SLGTAIGFRI++GVLT+I AILVFV+RKVHKQWL+P+QCLPTALEGPGGVWCDVDVV
Sbjct: 121 RRFSLGTAIGFRIRRGVLTNIAAILVFVARKVHKQWLNPLQCLPTALEGPGGVWCDVDVV 180

Query: 185 EFSYFGAPNPAPKEQLYTEIVDDLRGSDLCIGSGSQVASQETYGTLGAIVRSQTGSRQVG 244
           EF Y+GAP   PKEQ+YTE+VDDLRGS   IGSGSQVASQETYGTLGAIV+S+TG RQVG
Sbjct: 181 EFQYYGAPAQTPKEQVYTELVDDLRGSGSSIGSGSQVASQETYGTLGAIVKSKTGIRQVG 240

Query: 245 FLTNRHVAVDLDYPNQKMFHPLPPTLGPGVYL------------------------ETFV 304
           FLTNRHVAVDLDYP+QKMFHPLPP+LGPGVYL                        ETFV
Sbjct: 241 FLTNRHVAVDLDYPSQKMFHPLPPSLGPGVYLGAVERATSFITDDLWYGIFAGTNPETFV 300

Query: 305 RADGAFIPFADDFDMSTVTTSVKGVGEVGDVKFIDLQSPISTLIGKQVVKVGRSSGLTTG 364
           RADGAFIPFA+DF+ + VTT+VKG+GE+GD+   DLQSP+++LIG++VVKVGRSSGLTTG
Sbjct: 301 RADGAFIPFAEDFNTNNVTTTVKGIGEIGDIHATDLQSPVNSLIGRKVVKVGRSSGLTTG 360

Query: 365 TVLAYALEYNDEKGICFLTDFLVVGENQQTFDLEGDSGSLIILKG--ENRESLQPIGIIW 424
           T++AYALEYNDEKGICFLTDFLVVGENQQTFDLEGDSGSLI+L    E  E  +P+GIIW
Sbjct: 361 TIMAYALEYNDEKGICFLTDFLVVGENQQTFDLEGDSGSLILLAAGDEKNEKPRPVGIIW 420

Query: 425 GGTANRGRLKLKVGQPPENWTSGVDLGRLLNLLELDLITTDDGFKAAVQEQRT-VSATVI 484
           GGTANRGRLKLKVG+ PENWTSGVDLGR+LNLLELDLIT+++G +AAV EQR  +    +
Sbjct: 421 GGTANRGRLKLKVGEQPENWTSGVDLGRVLNLLELDLITSNEGLQAAVLEQRNGIMCAAV 480

Query: 485 GSIVGDSSPPDTTLPKDKSEEKFEPLGFQIQHMPTEVEPSSAKDRPLLETEFHLEAGTST 544
            S V +SSP    + + K+ E FEP+   +Q +   +E  ++   P  + E  LE   S 
Sbjct: 481 DSTVVESSPGVCNISRCKTGENFEPINLNVQQV--LIEDDNSNIHPEFQIEDVLE---SV 540

Query: 545 APSVEHQFIPSLFSCSPSLSNSTLG--RAVSQNLSSLR--SDCEDISVSLHLGDHEA-KR 581
           A   EHQFIPS  +   +L     G     S+NLSSL+  S  ++I  SL LG+ +  KR
Sbjct: 541 AVIEEHQFIPSSSNNGSALHQKPNGPENLESKNLSSLKTSSSGDEIGFSLQLGESDTKKR 600

BLAST of Carg14778 vs. TAIR 10
Match: AT2G35155.1 (Trypsin family protein )

HSP 1 Score: 700.7 bits (1807), Expect = 1.0e-201
Identity = 368/587 (62.69%), Postives = 435/587 (74.11%), Query Frame = 0

Query: 14  INCSGSTPSEESALDLERNCY-GHSNLPSFSPPT-LQPFASAGQHCESNAAYFSWPTPIR 73
           I  + S+ SE+SALDLERN +  H +LPS S P+ LQPF    QH ESNA YFSWPT  R
Sbjct: 11  IQAAASSESEDSALDLERNHHCNHLSLPSSSSPSPLQPFTLNIQHAESNAPYFSWPTLSR 70

Query: 74  LSVAAEERANYFANLQKGVLPDILRPLPKGQRATTLLELMTIRAFHSKILRCYSLGTAIG 133
           L+   E+RANYF NLQKGVLP+ +  LP GQ+ATTLLELMTIRAFHSKILR +SLGTA+G
Sbjct: 71  LNDTVEDRANYFGNLQKGVLPETVGRLPSGQQATTLLELMTIRAFHSKILRRFSLGTAVG 130

Query: 134 FRIQKGVLTDIPAILVFVSRKVHKQWLSPIQCLPTALEGPGGVWCDVDVVEFSYFGAPNP 193
           FRI +GVLT++PAILVFV+RKVH+QWL+P+QCLP+ALEGPGGVWCDVDVVEF Y+GAP  
Sbjct: 131 FRISRGVLTNVPAILVFVARKVHRQWLNPMQCLPSALEGPGGVWCDVDVVEFQYYGAPAA 190

Query: 194 APKEQLYTEIVDDLRGSDLCIGSGSQVASQETYGTLGAIVRSQTGSRQVGFLTNRHVAVD 253
            PKEQ+Y E+VD LRGSD CIGSGSQVASQETYGTLGAIV+S+TG+ QVGFLTNRHVAVD
Sbjct: 191 TPKEQVYNELVDGLRGSDPCIGSGSQVASQETYGTLGAIVKSRTGNHQVGFLTNRHVAVD 250

Query: 254 LDYPNQKMFHPLPPTLGPGVYL------------------------ETFVRADGAFIPFA 313
           LDYP+QKMFHPLPP+LGPGVYL                        ETFVRADGAFIPFA
Sbjct: 251 LDYPSQKMFHPLPPSLGPGVYLGAVERATSFITDDQWYGIFAGTNPETFVRADGAFIPFA 310

Query: 314 DDFDMSTVTTSVKGVGEVGDVKFIDLQSPISTLIGKQVVKVGRSSGLTTGTVLAYALEYN 373
           +DF+ S VTT +KG+GE+GDV  IDLQSPI +LIGKQVVKVGRSSG TTGT++AYALEYN
Sbjct: 311 EDFNTSNVTTLIKGIGEIGDVHVIDLQSPIDSLIGKQVVKVGRSSGYTTGTIMAYALEYN 370

Query: 374 DEKGICFLTDFLVVGENQQTFDLEGDSGSLIILKGENRESLQPIGIIWGGTANRGRLKLK 433
           DEKGICFLTDFLV+GENQQTFDLEGDSGSLI+L G N +  +P+GIIWGGTANRGRLKL 
Sbjct: 371 DEKGICFLTDFLVIGENQQTFDLEGDSGSLILLTGPNGQKPRPVGIIWGGTANRGRLKLI 430

Query: 434 VGQPPENWTSGVDLGRLLNLLELDLITTDDGFK--AAVQEQRTVSATVIGSIVGDSSPPD 493
            GQ PENWTSGVDLGRLL+LLELDLIT++   +  AA +E+R  S T + S V  SSPPD
Sbjct: 431 AGQEPENWTSGVDLGRLLDLLELDLITSNHELEAAAAAREERNTSVTALDSTVSQSSPPD 490

Query: 494 TTLPKDKSEEKFEPLGFQIQHMPTEVEPSSAKDRPLLETEFHLEAGTSTAPSVEHQFIPS 553
                DK +E FE                     P +  EFH+E        VE     +
Sbjct: 491 PVPSGDKQDESFE---------------------PFIPPEFHIEEAIKPTLEVEEHIFIA 550

Query: 554 LFSCSPSLSNSTLGRAVSQ--NLSSLR-SDCEDISVSLHLGDHEAKR 570
             S + S S +  G+ + +  NL +L+ S  E++++SLHLG+ + K+
Sbjct: 551 PISVNESTS-AIKGQEIPKLDNLMALKNSSEEEVNISLHLGEPKLKK 575

BLAST of Carg14778 vs. TAIR 10
Match: AT3G12950.1 (Trypsin family protein )

HSP 1 Score: 674.1 bits (1738), Expect = 1.0e-193
Identity = 371/566 (65.55%), Postives = 423/566 (74.73%), Query Frame = 0

Query: 50  FASAGQHCESNAA-YFSWPTPIRLSVAAEERANYFANLQK------GVLPDILRPLPKGQ 109
           + S GQHCE  AA YFSWPT  RLS AAEERANYF+NLQK       V P+ +   PKGQ
Sbjct: 4   YGSTGQHCEFTAASYFSWPTSSRLSNAAEERANYFSNLQKEEDDDDEVSPEPVSTEPKGQ 63

Query: 110 RATTLLELMTIRAFHSKILRCYSLGTAIGFRIQKGVLTDIPAILVFVSRKVHKQWLSPIQ 169
           RATTLLELMTIRAFHSK+LRCYSLGTAIGFRI++GVLTDIPAI+VFVSRKVHKQWLSP+Q
Sbjct: 64  RATTLLELMTIRAFHSKMLRCYSLGTAIGFRIRRGVLTDIPAIIVFVSRKVHKQWLSPLQ 123

Query: 170 CLPTALEGPGGVWCDVDVVEFSYFGAPN--PAPKEQLYTEIVDDLRGSDLCIGSGSQVAS 229
           CLPTALEG GG+WCDVDVVEFSYFG P+  P PK+   T+IVD L+GSD  IGSGSQVAS
Sbjct: 124 CLPTALEGAGGIWCDVDVVEFSYFGEPDHQPTPKQTFTTDIVDHLQGSDPFIGSGSQVAS 183

Query: 230 QETYGTLGAIVRSQTGSRQVGFLTNRHVAVDLDYPNQKMFHPLPPTLGPGVYL------- 289
           QET GTLGAIVRSQTG RQVGF+TNRHVAV+LDYP+QKMFHPLPP LGPGVYL       
Sbjct: 184 QETCGTLGAIVRSQTGGRQVGFVTNRHVAVNLDYPSQKMFHPLPPALGPGVYLGAVERAT 243

Query: 290 -----------------ETFVRADGAFIPFADDFDMSTVTTSVK-GVGEVGDVKFIDLQS 349
                            ETFVRADGAFIPFADD+D+S VTTSVK GVGE+G+VK I+LQS
Sbjct: 244 SFITDDLWFGIFAGTNPETFVRADGAFIPFADDYDLSRVTTSVKGGVGEIGEVKAIELQS 303

Query: 350 PISTLIGKQVVKVGRSSGLTTGTVLAYALEYNDEKGICFLTDFLVVGENQQT-FDLEGDS 409
           P+ +L+GKQVVKVGRSSGLTTGTVLAYALEYNDE+G+CFLTDFLVVGEN ++ FDLEGDS
Sbjct: 304 PVGSLVGKQVVKVGRSSGLTTGTVLAYALEYNDERGVCFLTDFLVVGENHRSPFDLEGDS 363

Query: 410 GSLIILKGENRESLQPIGIIWGGTANRGRLKLKVGQPPENWTSGVDLGRLLNLLELDLIT 469
           GSLI++KGE  E  +PIGIIWGGT +RGRLKLKVG+ PE+WT+GVDLGRLL  L+LDLIT
Sbjct: 364 GSLIVMKGE--EKARPIGIIWGGTGSRGRLKLKVGECPESWTTGVDLGRLLTHLQLDLIT 423

Query: 470 TDDGFKAAVQEQRTVSATVIGSIVGDSSPPDTTLPKDK--SEEKFE-PLG-FQIQHMPTE 529
           TD+G KAAVQEQR  S T + S+V DSSPP   L K+K   EEK E  LG  Q+QH+   
Sbjct: 424 TDEGLKAAVQEQRAASTTGMSSMVADSSPPYVNLKKEKRSPEEKLEASLGPLQVQHID-- 483

Query: 530 VEPSSAKDRPLLETEFHLEAGTSTAPSVEHQFIPSLF-SCSPSLSNSTLGRAVSQNLSSL 576
                      LE     + G   APSVEHQF+P+    CS S    T    +    ++ 
Sbjct: 484 -----------LEERIETKGG---APSVEHQFMPTFSGQCSASAWPETAREDLVAGFTNG 543

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
KAG7037486.10.0e+00100.00hypothetical protein SDJN02_01113, partial [Cucurbita argyrosperma subsp. argyro... [more]
KAG6607973.10.0e+0096.04Protein NARROW LEAF 1, partial [Cucurbita argyrosperma subsp. sororia][more]
XP_022981089.10.0e+0095.85uncharacterized protein LOC111480347 [Cucurbita maxima] >XP_023524995.1 uncharac... [more]
XP_022940289.10.0e+0095.51uncharacterized protein LOC111445958 [Cucurbita moschata][more]
XP_022136083.10.0e+0092.69uncharacterized protein LOC111007860 isoform X1 [Momordica charantia][more]
Match NameE-valueIdentityDescription
B4XT643.4e-19460.37Protein NARROW LEAF 1 OS=Oryza sativa subsp. japonica OX=39947 GN=NAL1 PE=1 SV=1[more]
Match NameE-valueIdentityDescription
A0A6J1J1090.0e+0095.85uncharacterized protein LOC111480347 OS=Cucurbita maxima OX=3661 GN=LOC111480347... [more]
A0A6J1FJM90.0e+0095.51uncharacterized protein LOC111445958 OS=Cucurbita moschata OX=3662 GN=LOC1114459... [more]
A0A6J1C6M70.0e+0092.69uncharacterized protein LOC111007860 isoform X1 OS=Momordica charantia OX=3673 G... [more]
A0A6J1C3A80.0e+0092.52uncharacterized protein LOC111007860 isoform X2 OS=Momordica charantia OX=3673 G... [more]
A0A6J1F9R81.6e-30889.88uncharacterized protein LOC111443385 OS=Cucurbita moschata OX=3662 GN=LOC1114433... [more]
Match NameE-valueIdentityDescription
AT5G45030.14.5e-20262.52Trypsin family protein [more]
AT5G45030.24.5e-20262.52Trypsin family protein [more]
AT2G35155.11.0e-20162.69Trypsin family protein [more]
AT3G12950.11.0e-19365.55Trypsin family protein [more]
InterPro
Analysis Name: InterPro Annotations of Silver-seed gourd (SMH-JMG-627) v2
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availablePANTHERPTHR31521:SF3BNAA01G30180D PROTEINcoord: 274..581
NoneNo IPR availablePANTHERPTHR31521EXPRESSED PROTEINcoord: 5..273
NoneNo IPR availablePANTHERPTHR31521:SF3BNAA01G30180D PROTEINcoord: 5..273
NoneNo IPR availablePANTHERPTHR31521EXPRESSED PROTEINcoord: 274..581
IPR009003Peptidase S1, PA clanSUPERFAMILY50494Trypsin-like serine proteasescoord: 218..405

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Carg14778-RACarg14778-RAmRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
cellular_component GO:0043231 intracellular membrane-bounded organelle