Carg14776 (gene) Silver-seed gourd (SMH-JMG-627) v2

Overview


Name	Carg14776
Type	gene
Organism	Cucurbita argyrosperma subsp. argyrosperma cv. SMH-JMG-627 (Silver-seed gourd (SMH-JMG-627) v2)
Description	Obg-like ATPase 1
Location	Carg_Chr01: 10359601 .. 10362177 (+)
RNA-Seq Expression	Carg14776
Synteny	Carg14776

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

CGTATACACTTTTTGATAATTTTGTTCATCCCAACCGATCTGTTTGGCTTTGGTCCGAGCTCTGCAACAGGTGGCCACAGCCATGGCCAGAATAGCTTGCGCTCACATTATTCCGTCTCTGCTTTCCCCTTCGAAATCCTTCAGTTAACTCGCACTCTCTCCTCAGGAGCTTCTCATTTCAGCCATGTTACGGTGGTTGGCCGTCGTTTCTCTGATACATATAAGTTCAGTACGAGCCTCAGAGCCGGCATCGTTGGCCTCATCAATGTTGGAAAATCAACGCTCTTCAATGTCGTCGTAAGCTAATATCGGTTGCTCTCTCTCTTTATTTCATACTAAATTTACTGTAATTTGGATTTCGAGCTATGGAACTGATGGTTACTTTCGGAAAACTCCGTAGCTACTGAAGAATTCGTTGTTGAATTGTCGTTATAGCTAGTGGATTGTGGAATTGTGCACCAGGATTTGTGAACTATTGGATTATAATGTAGGTAGTTTATAAACGCTACGGACTCACAGCTCTCTTCGAATTTTTCATGGTCTGATTATCGTATTTTTCATGTTAGGTTTTCTACATATCTTGTGCATCTTAAGGCTACGCCTCGAGATTTTGACACGATAGCTCCACATATTCACTCCGAGCGATGAATGTGTTTCAATAATTTGTTTAAAGTGATTTTTTCGGATCATCATCGCTTTTACTAATTTTGATACAAGCTACATCATAGTATATTTAATATGATGATAATCATGCTGTTTCTGGCTAACAATGCAGTGTCATGTGTCCGATTGTGAAAGCTCACTTAACTTCTAACTTCTTTCCACAGATTTAATTTTGTGAATTGGACGGCTTCCTCTTTTAGTTCATACTTCCACTTGGAAATCTACATCAATTTCATATGACGCGTATTGTCTATGATGGAAACCTTTGCAGGTTGAAAATGGCAAGGCTCAAGCAGCTAAATTTCCTTTCTGTACAATAGAGCCCAACGTAGGAGTAGTTGCAGTTCCAGATCCTCGTCTGCATCAACTTTCTGATCTCAGCAAGTCTATACGAGCGGTCCCAGCATCCATAGAGTTTGTAGATACTGCTGGGTTAATGAATGGTGCCAGTCAAGGAGAAGTATGGTTCATTCACACCTCTGTTGAGGATTGTTGGGAGTGAGTCCCACCATGGCTAATTTAGAGAATGATCATGAGTTTATAGGTAAAGGAATACATCTCCATTGGCATGAGGCCTTTTGGGGAAGCCTAAAGCAAAACCATGAGAGCTTATGCTCAAAGTGGACAATATCATATCATTGTGGAGAGTCGTGATTCTTAACACACCTCAGCGTTCTTTGACTAAGATTCCAAATGATTTTTGAATAAAGATGAAATTCATTGTACAGAAATGACAAGAAAACAAACACAATTTTCAAAAACAAAAAATTAAAAATTAAATGGTTGTGGAAATACACATTTTGAAAATTCATTTGTATGTATGTTTTCATTGATGTTATTTTCTAGAATTCTGTAATAGACTTCCCCTTTATTTTGACCACTGCAGGGATTGGGAATATAACTTCTTATCCCATGTTCGTGAGGTGGAGGTCCATACTTCAGGTGATTTTCTATTTTATCATATTTCTTTTGTCATAATACTTAGAAACCTACTTACATAATTGTATTTCTAAAGAGTAATACCGGTTTAACAGATGAATTGGCTAGTATATTTCTCTGCAATATCAACTGCCCGGTGATTCAACAAGAGAAAGAATGGACTTTAATTTGTTGAATTCTTTGTTTTTTTGTCTTACAGAATTGTGTAAGTTTTTGTTCCTATTTATCTACTTAACTTTTCTGTTATATATTTTTGTCCTATAATACGCATGCATGCCAAATAGCCAAAACAATGCCCTGGAAGCTCTAAGAATAACGTGGGCACTTCTTGCTTTTTTCATTACTTAATTTTGTGCTGTTAAATATTTTAATATTATTGTTCCAGGCTGTGCGCTGTTTTGAGGATAACGACATTGTTCACGTGAATGGGAAAATTGATCCAAAAACTGGTATTGATTTGATTAACTTAGAGCTTTATTTTTCAGATCTGGACCAGGTAACATCACTGAAACCTTTTCTATATTCATACGCATGTTTTGCACCTTTTAGTATGCAGGGTTACAATCCTTTGTTATTATCTTGCTACTTTTTATTTAATTGTACATTTGCCTTTTGTACTGCCGTTAACTGAATCCCTATATATTAGAGATATTAGGTAACAATAAATCTAAATAAATTCTTATCTAAAGCGAGCACTGGCTTTGTTTGCCTCTCTCGTGTTCACCCTTTTGGAGTACTTCCATGAGCATTACTGGCCTCTCTCATACTCACTCCACTTGGAGCACTTCTATTGGTATTGTTTGCCTCTCTAGTGCTCACTTCTTAAGGAAAATTGATTGATTAATGTTTGTGAAGAACTCCATCGCTTGCAATGGTGACTTCTTGAAGCAATTTTTTGAACTTCTAATCGTTCCTCACAGCTAGAAGAACATGCCTAGGGTTTATCCTAGTTTTCTAGCCAATTCCAAAATCTGA

mRNA sequence

CGTATACACTTTTTGATAATTTTGTTCATCCCAACCGATCTGTTTGGCTTTGGTCCGAGCTCTGCAACAGGTGGCCACAGCCATGGCCAGAATAGCTTGCGCTCACATTATTCCGTCTCTGCTTTCCCCTTCGAAATCCTTCAGTTAACTCGCACTCTCTCCTCAGGAGCTTCTCATTTCAGCCATGTTACGGTGGTTGGCCGTCGTTTCTCTGATACATATAAGTTCAGTACGAGCCTCAGAGCCGGCATCGTTGGCCTCATCAATGTTGGAAAATCAACGCTCTTCAATGTCGTCGTTGAAAATGGCAAGGCTCAAGCAGCTAAATTTCCTTTCTGTACAATAGAGCCCAACGTAGGAGTAGTTGCAGTTCCAGATCCTCGTCTGCATCAACTTTCTGATCTCAGCAAGTCTATACGAGCGGTCCCAGCATCCATAGAGGATTGGGAATATAACTTCTTATCCCATGTTCGTGAGGTGGAGGTCCATACTTCAGATGAATTGGCTAGTATATTTCTCTGCAATATCAACTGCCCGGCTGTGCGCTGTTTTGAGGATAACGACATTGTTCACGTGAATGGGAAAATTGATCCAAAAACTGGTATTGATTTGATTAACTTAGAGCTTTATTTTTCAGATCTGGACCAGAAGAACATGCCTAGGGTTTATCCTAGTTTTCTAGCCAATTCCAAAATCTGA

Coding sequence (CDS)

CGTATACACTTTTTGATAATTTTGTTCATCCCAACCGATCTGTTTGGCTTTGGTCCGAGCTCTGCAACAGGTGGCCACAGCCATGGCCAGAATAGCTTGCGCTCACATTATTCCGTCTCTGCTTTCCCCTTCGAAATCCTTCAGTTAACTCGCACTCTCTCCTCAGGAGCTTCTCATTTCAGCCATGTTACGGTGGTTGGCCGTCGTTTCTCTGATACATATAAGTTCAGTACGAGCCTCAGAGCCGGCATCGTTGGCCTCATCAATGTTGGAAAATCAACGCTCTTCAATGTCGTCGTTGAAAATGGCAAGGCTCAAGCAGCTAAATTTCCTTTCTGTACAATAGAGCCCAACGTAGGAGTAGTTGCAGTTCCAGATCCTCGTCTGCATCAACTTTCTGATCTCAGCAAGTCTATACGAGCGGTCCCAGCATCCATAGAGGATTGGGAATATAACTTCTTATCCCATGTTCGTGAGGTGGAGGTCCATACTTCAGATGAATTGGCTAGTATATTTCTCTGCAATATCAACTGCCCGGCTGTGCGCTGTTTTGAGGATAACGACATTGTTCACGTGAATGGGAAAATTGATCCAAAAACTGGTATTGATTTGATTAACTTAGAGCTTTATTTTTCAGATCTGGACCAGAAGAACATGCCTAGGGTTTATCCTAGTTTTCTAGCCAATTCCAAAATCTGA

Protein sequence

RIHFLIILFIPTDLFGFGPSSATGGHSHGQNSLRSHYSVSAFPFEILQLTRTLSSGASHFSHVTVVGRRFSDTYKFSTSLRAGIVGLINVGKSTLFNVVVENGKAQAAKFPFCTIEPNVGVVAVPDPRLHQLSDLSKSIRAVPASIEDWEYNFLSHVREVEVHTSDELASIFLCNINCPAVRCFEDNDIVHVNGKIDPKTGIDLINLELYFSDLDQKNMPRVYPSFLANSKI

Homology

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BLAST of Carg14776 vs. NCBI nr
Match: KAG7037484.1 (Ribosome-binding ATPase YchF, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 470.3 bits (1209), Expect = 9.5e-129
Identity = 232/232 (100.00%), Postives = 232/232 (100.00%), Query Frame = 0


Query: 1   RIHFLIILFIPTDLFGFGPSSATGGHSHGQNSLRSHYSVSAFPFEILQLTRTLSSGASHF 60
           RIHFLIILFIPTDLFGFGPSSATGGHSHGQNSLRSHYSVSAFPFEILQLTRTLSSGASHF
Sbjct: 1   RIHFLIILFIPTDLFGFGPSSATGGHSHGQNSLRSHYSVSAFPFEILQLTRTLSSGASHF 60

Query: 61  SHVTVVGRRFSDTYKFSTSLRAGIVGLINVGKSTLFNVVVENGKAQAAKFPFCTIEPNVG 120
           SHVTVVGRRFSDTYKFSTSLRAGIVGLINVGKSTLFNVVVENGKAQAAKFPFCTIEPNVG
Sbjct: 61  SHVTVVGRRFSDTYKFSTSLRAGIVGLINVGKSTLFNVVVENGKAQAAKFPFCTIEPNVG 120

Query: 121 VVAVPDPRLHQLSDLSKSIRAVPASIEDWEYNFLSHVREVEVHTSDELASIFLCNINCPA 180
           VVAVPDPRLHQLSDLSKSIRAVPASIEDWEYNFLSHVREVEVHTSDELASIFLCNINCPA
Sbjct: 121 VVAVPDPRLHQLSDLSKSIRAVPASIEDWEYNFLSHVREVEVHTSDELASIFLCNINCPA 180

Query: 181 VRCFEDNDIVHVNGKIDPKTGIDLINLELYFSDLDQKNMPRVYPSFLANSKI 233
           VRCFEDNDIVHVNGKIDPKTGIDLINLELYFSDLDQKNMPRVYPSFLANSKI
Sbjct: 181 VRCFEDNDIVHVNGKIDPKTGIDLINLELYFSDLDQKNMPRVYPSFLANSKI 232

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BLAST of Carg14776 vs. NCBI nr
Match: XP_022981111.1 (uncharacterized protein LOC111480358 isoform X1 [Cucurbita maxima])

HSP 1 Score: 237.7 bits (605), Expect = 1.0e-58
Identity = 130/164 (79.27%), Postives = 135/164 (82.32%), Query Frame = 0


Query: 34  RSHYSVSAFPFE-ILQLTRTLSSGASHFSHVTVVGRRFSDTYKFSTSLRAGIVGLINVGK 93
           RSHYSVSAFP + +LQL+RTL  GASH SHVTVVGRRFSDT K STSLRAGIVGLINVGK
Sbjct: 3   RSHYSVSAFPSKSLLQLSRTL--GASHLSHVTVVGRRFSDTSKISTSLRAGIVGLINVGK 62

Query: 94  STLFNVVVENGKAQAAKFPFCTIEPNVGVVAVPDPRLHQLSDLSKSIRAVPASIEDWE-- 153
           STLFN VVENGKAQAAK PFCTIEPNVGVVAVPDPRLHQLSDLSKSIRAVPASIE  +  
Sbjct: 63  STLFNAVVENGKAQAAKVPFCTIEPNVGVVAVPDPRLHQLSDLSKSIRAVPASIEFVDTS 122

Query: 154 -------------YNFLSHVREVEVHTSDELASIFLCNINCPAV 182
                         NFLSHV EVEVHTSDELASIFLCNINCP +
Sbjct: 123 GLVNGGSQGEGLGNNFLSHVHEVEVHTSDELASIFLCNINCPVI 164

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BLAST of Carg14776 vs. NCBI nr
Match: XP_038898080.1 (ribosome-binding ATPase YchF isoform X3 [Benincasa hispida])

HSP 1 Score: 219.2 bits (557), Expect = 3.8e-53
Identity = 122/182 (67.03%), Postives = 130/182 (71.43%), Query Frame = 0


Query: 50  TRTLSSGASHFSHVTVVGRRFSDTYKFSTSLRAGIVGLINVGKSTLFNVVVENGKAQAAK 109
           T +L S ASHF HV VVGRRFS   K S SLRAGIVGL NVGKSTLFN VVENGKAQAA 
Sbjct: 24  THSLFSRASHFDHVMVVGRRFSSASKISMSLRAGIVGLPNVGKSTLFNAVVENGKAQAAN 83

Query: 110 FPFCTIEPNVGVVAVPDPRLHQLSDLSKSIRAVPASIEDWEY---------------NFL 169
           FPFCTIEPNVGVVAVPDPRLH+LSDLSKS RAVPASIE  +                 FL
Sbjct: 84  FPFCTIEPNVGVVAVPDPRLHKLSDLSKSQRAVPASIEFVDIAGLVKGASQGEGLGNKFL 143

Query: 170 SHVREVEVHTSDELASIFLCNINCPAVRCFEDNDIVHVNGKIDPKTGIDLINLELYFSDL 217
           SH+REV     D +  +         VRCFEDNDI+HVNGKIDPKT ID+INLEL FSDL
Sbjct: 144 SHIREV-----DSILQV---------VRCFEDNDIIHVNGKIDPKTDIDMINLELVFSDL 191

[Close]

BLAST of Carg14776 vs. NCBI nr
Match: XP_038898079.1 (ribosome-binding ATPase YchF isoform X2 [Benincasa hispida])

HSP 1 Score: 219.2 bits (557), Expect = 3.8e-53
Identity = 122/182 (67.03%), Postives = 130/182 (71.43%), Query Frame = 0


Query: 50  TRTLSSGASHFSHVTVVGRRFSDTYKFSTSLRAGIVGLINVGKSTLFNVVVENGKAQAAK 109
           T +L S ASHF HV VVGRRFS   K S SLRAGIVGL NVGKSTLFN VVENGKAQAA 
Sbjct: 24  THSLFSRASHFDHVMVVGRRFSSASKISMSLRAGIVGLPNVGKSTLFNAVVENGKAQAAN 83

Query: 110 FPFCTIEPNVGVVAVPDPRLHQLSDLSKSIRAVPASIEDWEY---------------NFL 169
           FPFCTIEPNVGVVAVPDPRLH+LSDLSKS RAVPASIE  +                 FL
Sbjct: 84  FPFCTIEPNVGVVAVPDPRLHKLSDLSKSQRAVPASIEFVDIAGLVKGASQGEGLGNKFL 143

Query: 170 SHVREVEVHTSDELASIFLCNINCPAVRCFEDNDIVHVNGKIDPKTGIDLINLELYFSDL 217
           SH+REV     D +  +         VRCFEDNDI+HVNGKIDPKT ID+INLEL FSDL
Sbjct: 144 SHIREV-----DSILQV---------VRCFEDNDIIHVNGKIDPKTDIDMINLELVFSDL 191

[Close]

BLAST of Carg14776 vs. NCBI nr
Match: XP_038898078.1 (ribosome-binding ATPase YchF isoform X1 [Benincasa hispida])

HSP 1 Score: 219.2 bits (557), Expect = 3.8e-53
Identity = 122/182 (67.03%), Postives = 130/182 (71.43%), Query Frame = 0


Query: 50  TRTLSSGASHFSHVTVVGRRFSDTYKFSTSLRAGIVGLINVGKSTLFNVVVENGKAQAAK 109
           T +L S ASHF HV VVGRRFS   K S SLRAGIVGL NVGKSTLFN VVENGKAQAA 
Sbjct: 24  THSLFSRASHFDHVMVVGRRFSSASKISMSLRAGIVGLPNVGKSTLFNAVVENGKAQAAN 83

Query: 110 FPFCTIEPNVGVVAVPDPRLHQLSDLSKSIRAVPASIEDWEY---------------NFL 169
           FPFCTIEPNVGVVAVPDPRLH+LSDLSKS RAVPASIE  +                 FL
Sbjct: 84  FPFCTIEPNVGVVAVPDPRLHKLSDLSKSQRAVPASIEFVDIAGLVKGASQGEGLGNKFL 143

Query: 170 SHVREVEVHTSDELASIFLCNINCPAVRCFEDNDIVHVNGKIDPKTGIDLINLELYFSDL 217
           SH+REV     D +  +         VRCFEDNDI+HVNGKIDPKT ID+INLEL FSDL
Sbjct: 144 SHIREV-----DSILQV---------VRCFEDNDIIHVNGKIDPKTDIDMINLELVFSDL 191

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BLAST of Carg14776 vs. ExPASy Swiss-Prot
Match: P37518 (Ribosome-binding ATPase YchF OS=Bacillus subtilis (strain 168) OX=224308 GN=ychF PE=2 SV=1)

HSP 1 Score: 118.2 bits (295), Expect = 1.2e-25
Identity = 71/170 (41.76%), Postives = 98/170 (57.65%), Query Frame = 0


Query: 79  SLRAGIVGLINVGKSTLFNVVVENGKAQAAKFPFCTIEPNVGVVAVPDPRLHQLSDLSKS 138
           +L AGIVGL NVGKSTLFN + + G A++A +PFCTI+PNVG+V VPD RL +L++L   
Sbjct: 2   ALTAGIVGLPNVGKSTLFNAITQAG-AESANYPFCTIDPNVGIVEVPDDRLQKLTELVNP 61

Query: 139 IRAVPASIEDWEY---------------NFLSHVREVEVHTSDELASIFLCNINCPAVRC 198
            + VP + E  +                 FLSH+R+V+                C  VR 
Sbjct: 62  KKTVPTAFEFTDIAGIVKGASKGEGLGNKFLSHIRQVDA--------------ICHVVRA 121

Query: 199 FEDNDIVHVNGKIDPKTGIDLINLELYFSDLD--QKNMPRVYPSFLANSK 232
           F D++I HV+GK+DP   I+ INLEL  +D++  +K + RV  S LA  K
Sbjct: 122 FSDDNITHVSGKVDPIDDIETINLELILADMETVEKRITRV--SKLAKQK 154

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BLAST of Carg14776 vs. ExPASy Swiss-Prot
Match: P0ABU3 (Ribosome-binding ATPase YchF OS=Escherichia coli O157:H7 OX=83334 GN=ychF PE=3 SV=2)

HSP 1 Score: 117.5 bits (293), Expect = 2.1e-25
Identity = 67/141 (47.52%), Postives = 92/141 (65.25%), Query Frame = 0


Query: 81  RAGIVGLINVGKSTLFNVVVENGKAQAAKFPFCTIEPNVGVVAVPDPRLHQLSDLSKSIR 140
           + GIVGL NVGKSTLFN + + G  +AA FPFCTIEPN GVV +PDPRL QL+++ K  R
Sbjct: 4   KCGIVGLPNVGKSTLFNALTKAG-IEAANFPFCTIEPNTGVVPMPDPRLDQLAEIVKPQR 63

Query: 141 AVPASIEDWEYNFLSHVREVEVHTSDE-LASIFLCNIN-----CPAVRCFEDNDIVHVNG 200
            +P ++E     F+     V+  +  E L + FL NI         VRCFE+++I+HV+G
Sbjct: 64  TLPTTME-----FVDIAGLVKGASKGEGLGNQFLTNIRETEAIGHVVRCFENDNIIHVSG 123

Query: 201 KIDPKTGIDLINLELYFSDLD 216
           K++P   I++IN EL  +DLD
Sbjct: 124 KVNPADDIEVINTELALADLD 138

[Close]

BLAST of Carg14776 vs. ExPASy Swiss-Prot
Match: P0ABU2 (Ribosome-binding ATPase YchF OS=Escherichia coli (strain K12) OX=83333 GN=ychF PE=1 SV=2)

HSP 1 Score: 117.5 bits (293), Expect = 2.1e-25
Identity = 67/141 (47.52%), Postives = 92/141 (65.25%), Query Frame = 0


Query: 81  RAGIVGLINVGKSTLFNVVVENGKAQAAKFPFCTIEPNVGVVAVPDPRLHQLSDLSKSIR 140
           + GIVGL NVGKSTLFN + + G  +AA FPFCTIEPN GVV +PDPRL QL+++ K  R
Sbjct: 4   KCGIVGLPNVGKSTLFNALTKAG-IEAANFPFCTIEPNTGVVPMPDPRLDQLAEIVKPQR 63

Query: 141 AVPASIEDWEYNFLSHVREVEVHTSDE-LASIFLCNIN-----CPAVRCFEDNDIVHVNG 200
            +P ++E     F+     V+  +  E L + FL NI         VRCFE+++I+HV+G
Sbjct: 64  TLPTTME-----FVDIAGLVKGASKGEGLGNQFLTNIRETEAIGHVVRCFENDNIIHVSG 123

Query: 201 KIDPKTGIDLINLELYFSDLD 216
           K++P   I++IN EL  +DLD
Sbjct: 124 KVNPADDIEVINTELALADLD 138

[Close]

BLAST of Carg14776 vs. ExPASy Swiss-Prot
Match: P0ABU4 (Ribosome-binding ATPase YchF OS=Shigella flexneri OX=623 GN=ychF PE=3 SV=2)

HSP 1 Score: 117.5 bits (293), Expect = 2.1e-25
Identity = 67/141 (47.52%), Postives = 92/141 (65.25%), Query Frame = 0


Query: 81  RAGIVGLINVGKSTLFNVVVENGKAQAAKFPFCTIEPNVGVVAVPDPRLHQLSDLSKSIR 140
           + GIVGL NVGKSTLFN + + G  +AA FPFCTIEPN GVV +PDPRL QL+++ K  R
Sbjct: 4   KCGIVGLPNVGKSTLFNALTKAG-IEAANFPFCTIEPNTGVVPMPDPRLDQLAEIVKPQR 63

Query: 141 AVPASIEDWEYNFLSHVREVEVHTSDE-LASIFLCNIN-----CPAVRCFEDNDIVHVNG 200
            +P ++E     F+     V+  +  E L + FL NI         VRCFE+++I+HV+G
Sbjct: 64  TLPTTME-----FVDIAGLVKGASKGEGLGNQFLTNIRETEAIGHVVRCFENDNIIHVSG 123

Query: 201 KIDPKTGIDLINLELYFSDLD 216
           K++P   I++IN EL  +DLD
Sbjct: 124 KVNPADDIEVINTELALADLD 138

[Close]

BLAST of Carg14776 vs. ExPASy Swiss-Prot
Match: P44681 (Ribosome-binding ATPase YchF OS=Haemophilus influenzae (strain ATCC 51907 / DSM 11121 / KW20 / Rd) OX=71421 GN=ychF PE=1 SV=2)

HSP 1 Score: 117.1 bits (292), Expect = 2.7e-25
Identity = 70/141 (49.65%), Postives = 88/141 (62.41%), Query Frame = 0


Query: 81  RAGIVGLINVGKSTLFNVVVENGKAQAAKFPFCTIEPNVGVVAVPDPRLHQLSDLSKSIR 140
           + GIVGL NVGKSTLFN + + G  +AA +PFCTIEPN GVV +PDPRL  L+++ K  R
Sbjct: 4   KCGIVGLPNVGKSTLFNALTKAG-IEAANYPFCTIEPNTGVVPMPDPRLDALAEIVKPER 63

Query: 141 AVPASIEDWEYNFLSHVREVEVHTSDE-LASIFLCNIN-----CPAVRCFEDNDIVHVNG 200
            +P ++E     F+     V   +  E L + FL NI         VRCFE++DIVHV G
Sbjct: 64  ILPTTME-----FVDIAGLVAGASKGEGLGNKFLANIRETDAIGHVVRCFENDDIVHVAG 123

Query: 201 KIDPKTGIDLINLELYFSDLD 216
           KIDP   ID IN EL  +DLD
Sbjct: 124 KIDPLDDIDTINTELALADLD 138

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BLAST of Carg14776 vs. ExPASy TrEMBL
Match: A0A6J1IVM0 (uncharacterized protein LOC111480358 isoform X1 OS=Cucurbita maxima OX=3661 GN=LOC111480358 PE=4 SV=1)

HSP 1 Score: 237.7 bits (605), Expect = 5.0e-59
Identity = 130/164 (79.27%), Postives = 135/164 (82.32%), Query Frame = 0


Query: 34  RSHYSVSAFPFE-ILQLTRTLSSGASHFSHVTVVGRRFSDTYKFSTSLRAGIVGLINVGK 93
           RSHYSVSAFP + +LQL+RTL  GASH SHVTVVGRRFSDT K STSLRAGIVGLINVGK
Sbjct: 3   RSHYSVSAFPSKSLLQLSRTL--GASHLSHVTVVGRRFSDTSKISTSLRAGIVGLINVGK 62

Query: 94  STLFNVVVENGKAQAAKFPFCTIEPNVGVVAVPDPRLHQLSDLSKSIRAVPASIEDWE-- 153
           STLFN VVENGKAQAAK PFCTIEPNVGVVAVPDPRLHQLSDLSKSIRAVPASIE  +  
Sbjct: 63  STLFNAVVENGKAQAAKVPFCTIEPNVGVVAVPDPRLHQLSDLSKSIRAVPASIEFVDTS 122

Query: 154 -------------YNFLSHVREVEVHTSDELASIFLCNINCPAV 182
                         NFLSHV EVEVHTSDELASIFLCNINCP +
Sbjct: 123 GLVNGGSQGEGLGNNFLSHVHEVEVHTSDELASIFLCNINCPVI 164

[Close]

BLAST of Carg14776 vs. ExPASy TrEMBL
Match: A0A6J1FB19 (Obg-like ATPase 1 OS=Cucurbita moschata OX=3662 GN=LOC111443686 PE=3 SV=1)

HSP 1 Score: 215.7 bits (548), Expect = 2.0e-52
Identity = 122/182 (67.03%), Postives = 130/182 (71.43%), Query Frame = 0


Query: 50  TRTLSSGASHFSHVTVVGRRFSDTYKFSTSLRAGIVGLINVGKSTLFNVVVENGKAQAAK 109
           T +L S ASHF  V VVGRRFS + K S SLRAGIVGL NVGKSTLFN VVENGKAQAA 
Sbjct: 24  TPSLFSRASHFDRVMVVGRRFSSSSKISMSLRAGIVGLPNVGKSTLFNAVVENGKAQAAN 83

Query: 110 FPFCTIEPNVGVVAVPDPRLHQLSDLSKSIRAVPASIEDWEY---------------NFL 169
           FPFCTIEPNVGVVAVPDPRLH+LSDLSKS RAVPASIE  +                 FL
Sbjct: 84  FPFCTIEPNVGVVAVPDPRLHKLSDLSKSQRAVPASIEFVDIAGLVKGASQGEGLGNKFL 143

Query: 170 SHVREVEVHTSDELASIFLCNINCPAVRCFEDNDIVHVNGKIDPKTGIDLINLELYFSDL 217
           SH+REV     D +  +         VRCFEDNDIVHVNGKIDPKT ID+INLEL FSDL
Sbjct: 144 SHIREV-----DSILQV---------VRCFEDNDIVHVNGKIDPKTDIDVINLELVFSDL 191

[Close]

BLAST of Carg14776 vs. ExPASy TrEMBL
Match: A0A6J1IHV4 (Obg-like ATPase 1 OS=Cucurbita maxima OX=3661 GN=LOC111477062 PE=3 SV=1)

HSP 1 Score: 213.0 bits (541), Expect = 1.3e-51
Identity = 120/182 (65.93%), Postives = 130/182 (71.43%), Query Frame = 0


Query: 50  TRTLSSGASHFSHVTVVGRRFSDTYKFSTSLRAGIVGLINVGKSTLFNVVVENGKAQAAK 109
           T +L S ASHF  V VVGRRFS + K S SLRAGIVGL NVGKSTLFN VVENG+AQAA 
Sbjct: 24  TPSLFSRASHFDRVMVVGRRFSSSSKISMSLRAGIVGLPNVGKSTLFNAVVENGRAQAAN 83

Query: 110 FPFCTIEPNVGVVAVPDPRLHQLSDLSKSIRAVPASIEDWEY---------------NFL 169
           FPFCTIEPNVGVVAVPDPRLH+LS+LSKS RAVPASIE  +                 FL
Sbjct: 84  FPFCTIEPNVGVVAVPDPRLHKLSELSKSQRAVPASIEFVDIAGLVKGASQGEGLGNKFL 143

Query: 170 SHVREVEVHTSDELASIFLCNINCPAVRCFEDNDIVHVNGKIDPKTGIDLINLELYFSDL 217
           SH+REV     D +  +         VRCFEDNDIVHVNGKIDPKT ID+INLEL FSDL
Sbjct: 144 SHIREV-----DSILQV---------VRCFEDNDIVHVNGKIDPKTDIDVINLELVFSDL 191

[Close]

BLAST of Carg14776 vs. ExPASy TrEMBL
Match: A0A6J1CLP3 (Obg-like ATPase 1 OS=Momordica charantia OX=3673 GN=LOC111012101 PE=3 SV=1)

HSP 1 Score: 213.0 bits (541), Expect = 1.3e-51
Identity = 120/182 (65.93%), Postives = 128/182 (70.33%), Query Frame = 0


Query: 50  TRTLSSGASHFSHVTVVGRRFSDTYKFSTSLRAGIVGLINVGKSTLFNVVVENGKAQAAK 109
           T +L S ASHF  V VVGRRFS   K S SLRAGIVGL NVGKSTLFN VVENGKAQAA 
Sbjct: 24  TPSLFSRASHFDRVMVVGRRFSSASKISMSLRAGIVGLPNVGKSTLFNAVVENGKAQAAN 83

Query: 110 FPFCTIEPNVGVVAVPDPRLHQLSDLSKSIRAVPASIEDWEY---------------NFL 169
           FPFCTIEPNVG+VAVPDPRLH+LSDLSKS RAVPASIE  +                 FL
Sbjct: 84  FPFCTIEPNVGIVAVPDPRLHKLSDLSKSQRAVPASIEFVDIAGLVKGASQGEGLGNKFL 143

Query: 170 SHVREVEVHTSDELASIFLCNINCPAVRCFEDNDIVHVNGKIDPKTGIDLINLELYFSDL 217
           SH+REV     D +  +         VRCFEDNDIVHVNGKIDPK  ID+INLEL FSDL
Sbjct: 144 SHIREV-----DSILQV---------VRCFEDNDIVHVNGKIDPKADIDVINLELVFSDL 191

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BLAST of Carg14776 vs. ExPASy TrEMBL
Match: A0A5A7UAY0 (Ribosome-binding ATPase YchF OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold207G001780 PE=4 SV=1)

HSP 1 Score: 211.1 bits (536), Expect = 5.0e-51
Identity = 119/182 (65.38%), Postives = 128/182 (70.33%), Query Frame = 0


Query: 50  TRTLSSGASHFSHVTVVGRRFSDTYKFSTSLRAGIVGLINVGKSTLFNVVVENGKAQAAK 109
           T +L S  SHF HV V+GRRFS + K S SLRAGIVGL NVGKSTLFN VVENGKAQAA 
Sbjct: 24  THSLFSRPSHFDHVMVLGRRFSSSSKISMSLRAGIVGLPNVGKSTLFNAVVENGKAQAAN 83

Query: 110 FPFCTIEPNVGVVAVPDPRLHQLSDLSKSIRAVPASIEDWEY---------------NFL 169
           FPFCTIEPNVGVVAVPDPRLH+LSDLSKS RAV ASIE  +                 FL
Sbjct: 84  FPFCTIEPNVGVVAVPDPRLHKLSDLSKSQRAVAASIEFVDIAGLVKGASQGEGLGNKFL 143

Query: 170 SHVREVEVHTSDELASIFLCNINCPAVRCFEDNDIVHVNGKIDPKTGIDLINLELYFSDL 217
           SH+REV     D +  +         VRCFEDNDIVHVNGKIDPKT ID+INLEL  SDL
Sbjct: 144 SHIREV-----DSILQV---------VRCFEDNDIVHVNGKIDPKTDIDVINLELVLSDL 191

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BLAST of Carg14776 vs. TAIR 10
Match: AT1G56050.1 (GTP-binding protein-related )

HSP 1 Score: 172.9 bits (437), Expect = 2.9e-43
Identity = 103/186 (55.38%), Postives = 119/186 (63.98%), Query Frame = 0


Query: 46  ILQLTRTLSSGASHFSHVTVVGRRFSDTYKFSTSLRAGIVGLINVGKSTLFNVVVENGKA 105
           +L   + L SG S F  V  + +R   + K S SL+AGIVGL NVGKSTLFN VVENGKA
Sbjct: 21  LLCRNQLLFSGNSKFVGVLTLQKRCFSS-KVSMSLKAGIVGLPNVGKSTLFNAVVENGKA 80

Query: 106 QAAKFPFCTIEPNVGVVAVPDPRLHQLSDLSKSIRAVPASIEDWEY-------------- 165
           QAA FPFCTIEPNVG+VAVPD RL  LS LS S + VPASIE  +               
Sbjct: 81  QAANFPFCTIEPNVGIVAVPDSRLQVLSKLSGSQKTVPASIEFVDIAGLVKGASQGEGLG 140

Query: 166 -NFLSHVREVEVHTSDELASIFLCNINCPAVRCFEDNDIVHVNGKIDPKTGIDLINLELY 217
             FLSH+REV     D +  +         VRCFEDNDI+HVNGK+DPK+ ID+INLEL 
Sbjct: 141 NKFLSHIREV-----DSILQV---------VRCFEDNDIIHVNGKVDPKSDIDVINLELI 191

[Close]

BLAST of Carg14776 vs. TAIR 10
Match: AT1G30580.1 (GTP binding )

HSP 1 Score: 90.5 bits (223), Expect = 1.9e-18
Identity = 59/156 (37.82%), Postives = 78/156 (50.00%), Query Frame = 0


Query: 75  KFSTSLRAGIVGLINVGKSTLFNVVVENGKAQAAKFPFCTIEPNVGVVAVPDPRLHQLSD 134
           +FS+ L+ GIVGL NVGKSTLFN + +     A  FPFCTIEPN   V +PD R   L  
Sbjct: 20  RFSSHLKIGIVGLPNVGKSTLFNTLTKL-SIPAENFPFCTIEPNEARVNIPDERFDWLCQ 79

Query: 135 LSKSIRAVPASIEDWEY---------------NFLSHVREVEVHTSDELASIFLCNINCP 194
             K    +PA +E  +                NFLSH+R V+         IF       
Sbjct: 80  TYKPKSEIPAFLEIHDIAGLVRGAHEGQGLGNNFLSHIRAVD--------GIF------H 139

Query: 195 AVRCFEDNDIVHVNGKIDPKTGIDLINLELYFSDLD 216
            +R FED DI+HV+  +DP   ++ I  EL   D++
Sbjct: 140 VLRAFEDADIIHVDDIVDPVRDLETITEELRLKDIE 160

The following BLAST results are available for this feature:

BLAST of Carg14776 vs. NCBI nr


Match Name	E-value	Identity	Description
KAG7037484.1	9.5e-129	100.00	Ribosome-binding ATPase YchF, partial [Cucurbita argyrosperma subsp. argyrosperm...	[more]
XP_022981111.1	1.0e-58	79.27	uncharacterized protein LOC111480358 isoform X1 [Cucurbita maxima]	[more]
XP_038898080.1	3.8e-53	67.03	ribosome-binding ATPase YchF isoform X3 [Benincasa hispida]	[more]
XP_038898079.1	3.8e-53	67.03	ribosome-binding ATPase YchF isoform X2 [Benincasa hispida]	[more]
XP_038898078.1	3.8e-53	67.03	ribosome-binding ATPase YchF isoform X1 [Benincasa hispida]	[more]

BLAST of Carg14776 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
P37518	1.2e-25	41.76	Ribosome-binding ATPase YchF OS=Bacillus subtilis (strain 168) OX=224308 GN=ychF...	[more]
P0ABU3	2.1e-25	47.52	Ribosome-binding ATPase YchF OS=Escherichia coli O157:H7 OX=83334 GN=ychF PE=3 S...	[more]
P0ABU2	2.1e-25	47.52	Ribosome-binding ATPase YchF OS=Escherichia coli (strain K12) OX=83333 GN=ychF P...	[more]
P0ABU4	2.1e-25	47.52	Ribosome-binding ATPase YchF OS=Shigella flexneri OX=623 GN=ychF PE=3 SV=2	[more]
P44681	2.7e-25	49.65	Ribosome-binding ATPase YchF OS=Haemophilus influenzae (strain ATCC 51907 / DSM ...	[more]

BLAST of Carg14776 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1IVM0	5.0e-59	79.27	uncharacterized protein LOC111480358 isoform X1 OS=Cucurbita maxima OX=3661 GN=L...	[more]
A0A6J1FB19	2.0e-52	67.03	Obg-like ATPase 1 OS=Cucurbita moschata OX=3662 GN=LOC111443686 PE=3 SV=1	[more]
A0A6J1IHV4	1.3e-51	65.93	Obg-like ATPase 1 OS=Cucurbita maxima OX=3661 GN=LOC111477062 PE=3 SV=1	[more]
A0A6J1CLP3	1.3e-51	65.93	Obg-like ATPase 1 OS=Momordica charantia OX=3673 GN=LOC111012101 PE=3 SV=1	[more]
A0A5A7UAY0	5.0e-51	65.38	Ribosome-binding ATPase YchF OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_sca...	[more]

BLAST of Carg14776 vs. TAIR 10


Match Name	E-value	Identity	Description
AT1G56050.1	2.9e-43	55.38	GTP-binding protein-related	[more]
AT1G30580.1	1.9e-18	37.82	GTP binding	[more]

InterPro

Analysis Name: InterPro Annotations of Silver-seed gourd (SMH-JMG-627) v2
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR006073	GTP binding domain	PRINTS	PR00326	GTP1OBG	coord: 104..122 score: 43.47 coord: 82..102 score: 48.11
IPR006073	GTP binding domain	PFAM	PF01926	MMR_HSR1	coord: 82..134 e-value: 9.0E-11 score: 41.9
IPR027417	P-loop containing nucleoside triphosphate hydrolase	GENE3D	3.40.50.300		coord: 81..165 e-value: 2.1E-17 score: 65.7
IPR027417	P-loop containing nucleoside triphosphate hydrolase	SUPERFAMILY	52540	P-loop containing nucleoside triphosphate hydrolases	coord: 77..218
IPR023192	TGS-like domain superfamily	GENE3D	1.10.150.300		coord: 187..220 e-value: 5.3E-5 score: 25.6
None	No IPR available	PANTHER	PTHR23305:SF18	OBG-LIKE ATPASE 1	coord: 79..216
None	No IPR available	PANTHER	PTHR23305	OBG GTPASE FAMILY	coord: 79..216

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Carg14776-RA	Carg14776-RA	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
cellular_component	GO:0005737	cytoplasm
cellular_component	GO:0016021	integral component of membrane
molecular_function	GO:0016887	ATP hydrolysis activity
molecular_function	GO:0005525	GTP binding
molecular_function	GO:0043021	ribonucleoprotein complex binding