Carg14758 (gene) Silver-seed gourd (SMH-JMG-627) v2

Overview


Name	Carg14758
Type	gene
Organism	Cucurbita argyrosperma subsp. argyrosperma cv. SMH-JMG-627 (Silver-seed gourd (SMH-JMG-627) v2)
Description	glucan endo-1,3-beta-glucosidase-like
Location	Carg_Chr01: 10229290 .. 10230145 (+)
RNA-Seq Expression	Carg14758
Synteny	Carg14758

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRCDSpolypeptidethree_prime_UTR

Hold the cursor over a type above to highlight its positions in the sequence below.

TTCTCCACTTTTTCCAATCATCTCACACTCCAAATCGCCTTTTTCCTCATCGCTTTTCTCCAGATCTCTGCCTCTTCCTCCAATGGCGAAAGCAGCCGCAGTCGCTCTCTCCTTTCTGCTTCTGTTCCTCTCAGGTTCGTTCTCCTGACCTCTTCGAATTCCGCGTCGTTTTTTGCGTACCTAACTTTCCTTCTTTCTTCTTTCTTCTTCCAATTCTTTTTTCAGGAGATCTCTTGGCGGTAAATGGATATCAGGTGAATCGATTGTTATAGAAATGCAGAGCAACAGATTAGAATAATCCGATGAAATCTGAATGATTTTTGTTTTTTGTTTTTGTTTGCAGAAGACGTGGTGCGTTGCGAGGCCTTCGTCGGATCACGCGACGCTGTTAGCGAATATCAATTATGCTTGTTCACAAGTGGATTGCAGGATTATGCAGAAAGGATGCCCCTGCTTTTATCCTGATAATCTGATTAACCATGCCTCCATCGCTATGAATCTGTATTACAATTCGAAAGGGAAGAATCAGTGGAACTGTGATTTCAGAGGATCTGGTCTCATCGTCATCACTGATCCAAGTTAGTTCATGATTTAAACAGATTTTGCTGTTGTGTTTGTCCTTTTGTTCTTGATTTTGATTTTGTGGTTCGATTTTCGCTGCTGCAGGTTATGGGAACTGCCTTTACCCTTGAATTGAAGTTTGAAGCATAGAAGTGTAGGAGAATCACACAGCTCAAAAGTAAATTTTATTGTTAGTTTATTGATTATATATACTTAGGTGGGAAGAGATAGTTACTGAATTAATGCTATTTATCTTTGATTCAATTCAATGCCAAAATTAAATTAAATTAGGGTT

mRNA sequence

TTCTCCACTTTTTCCAATCATCTCACACTCCAAATCGCCTTTTTCCTCATCGCTTTTCTCCAGATCTCTGCCTCTTCCTCCAATGGCGAAAGCAGCCGCAGTCGCTCTCTCCTTTCTGCTTCTGTTCCTCTCAGGAGATCTCTTGGCGGTAAATGGATATCAGAAGACGTGGTGCGTTGCGAGGCCTTCGTCGGATCACGCGACGCTGTTAGCGAATATCAATTATGCTTGTTCACAAGTGGATTGCAGGATTATGCAGAAAGGATGCCCCTGCTTTTATCCTGATAATCTGATTAACCATGCCTCCATCGCTATGAATCTGTATTACAATTCGAAAGGGAAGAATCAGTGGAACTGTGATTTCAGAGGATCTGGTCTCATCGTCATCACTGATCCAAGTTATGGGAACTGCCTTTACCCTTGAATTGAAGTTTGAAGCATAGAAGTGTAGGAGAATCACACAGCTCAAAAGTAAATTTTATTGTTAGTTTATTGATTATATATACTTAGGTGGGAAGAGATAGTTACTGAATTAATGCTATTTATCTTTGATTCAATTCAATGCCAAAATTAAATTAAATTAGGGTT

Coding sequence (CDS)

ATGGCGAAAGCAGCCGCAGTCGCTCTCTCCTTTCTGCTTCTGTTCCTCTCAGGAGATCTCTTGGCGGTAAATGGATATCAGAAGACGTGGTGCGTTGCGAGGCCTTCGTCGGATCACGCGACGCTGTTAGCGAATATCAATTATGCTTGTTCACAAGTGGATTGCAGGATTATGCAGAAAGGATGCCCCTGCTTTTATCCTGATAATCTGATTAACCATGCCTCCATCGCTATGAATCTGTATTACAATTCGAAAGGGAAGAATCAGTGGAACTGTGATTTCAGAGGATCTGGTCTCATCGTCATCACTGATCCAAGTTATGGGAACTGCCTTTACCCTTGA

Protein sequence

MAKAAAVALSFLLLFLSGDLLAVNGYQKTWCVARPSSDHATLLANINYACSQVDCRIMQKGCPCFYPDNLINHASIAMNLYYNSKGKNQWNCDFRGSGLIVITDPSYGNCLYP

Homology

[Close]

BLAST of Carg14758 vs. NCBI nr
Match: XP_022941529.1 (glucan endo-1,3-beta-glucosidase-like isoform X1 [Cucurbita moschata] >KAG7037466.1 Glucan endo-1,3-beta-D-glucosidase [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 243.8 bits (621), Expect = 7.1e-61
Identity = 113/113 (100.00%), Postives = 113/113 (100.00%), Query Frame = 0


Query: 1   MAKAAAVALSFLLLFLSGDLLAVNGYQKTWCVARPSSDHATLLANINYACSQVDCRIMQK 60
           MAKAAAVALSFLLLFLSGDLLAVNGYQKTWCVARPSSDHATLLANINYACSQVDCRIMQK
Sbjct: 1   MAKAAAVALSFLLLFLSGDLLAVNGYQKTWCVARPSSDHATLLANINYACSQVDCRIMQK 60

Query: 61  GCPCFYPDNLINHASIAMNLYYNSKGKNQWNCDFRGSGLIVITDPSYGNCLYP 114
           GCPCFYPDNLINHASIAMNLYYNSKGKNQWNCDFRGSGLIVITDPSYGNCLYP
Sbjct: 61  GCPCFYPDNLINHASIAMNLYYNSKGKNQWNCDFRGSGLIVITDPSYGNCLYP 113

[Close]

BLAST of Carg14758 vs. NCBI nr
Match: KAG6607951.1 (Glucan endo-1,3-beta-D-glucosidase, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 243.0 bits (619), Expect = 1.2e-60
Identity = 112/113 (99.12%), Postives = 113/113 (100.00%), Query Frame = 0


Query: 1   MAKAAAVALSFLLLFLSGDLLAVNGYQKTWCVARPSSDHATLLANINYACSQVDCRIMQK 60
           MAKAAAVA+SFLLLFLSGDLLAVNGYQKTWCVARPSSDHATLLANINYACSQVDCRIMQK
Sbjct: 1   MAKAAAVAISFLLLFLSGDLLAVNGYQKTWCVARPSSDHATLLANINYACSQVDCRIMQK 60

Query: 61  GCPCFYPDNLINHASIAMNLYYNSKGKNQWNCDFRGSGLIVITDPSYGNCLYP 114
           GCPCFYPDNLINHASIAMNLYYNSKGKNQWNCDFRGSGLIVITDPSYGNCLYP
Sbjct: 61  GCPCFYPDNLINHASIAMNLYYNSKGKNQWNCDFRGSGLIVITDPSYGNCLYP 113

[Close]

BLAST of Carg14758 vs. NCBI nr
Match: XP_022982185.1 (major pollen allergen Ole e 10-like isoform X1 [Cucurbita maxima])

HSP 1 Score: 238.4 bits (607), Expect = 3.0e-59
Identity = 110/113 (97.35%), Postives = 111/113 (98.23%), Query Frame = 0


Query: 1   MAKAAAVALSFLLLFLSGDLLAVNGYQKTWCVARPSSDHATLLANINYACSQVDCRIMQK 60
           MAKAAAV LSFLLLFLSGDLLAVNGYQKTWCV RPSSDHATLLANI+YACSQVDCRIMQK
Sbjct: 1   MAKAAAVTLSFLLLFLSGDLLAVNGYQKTWCVPRPSSDHATLLANIDYACSQVDCRIMQK 60

Query: 61  GCPCFYPDNLINHASIAMNLYYNSKGKNQWNCDFRGSGLIVITDPSYGNCLYP 114
           GCPCFYPDNLINHASIAMNLYYNSKGKNQWNCDFRGSGLIVITDPSYGNCLYP
Sbjct: 61  GCPCFYPDNLINHASIAMNLYYNSKGKNQWNCDFRGSGLIVITDPSYGNCLYP 113

[Close]

BLAST of Carg14758 vs. NCBI nr
Match: XP_022941530.1 (major pollen allergen Ole e 10-like isoform X2 [Cucurbita moschata])

HSP 1 Score: 236.9 bits (603), Expect = 8.6e-59
Identity = 112/113 (99.12%), Postives = 112/113 (99.12%), Query Frame = 0


Query: 1   MAKAAAVALSFLLLFLSGDLLAVNGYQKTWCVARPSSDHATLLANINYACSQVDCRIMQK 60
           MAKAAAVALSFLLLFLSGDLLAVNGYQ TWCVARPSSDHATLLANINYACSQVDCRIMQK
Sbjct: 1   MAKAAAVALSFLLLFLSGDLLAVNGYQ-TWCVARPSSDHATLLANINYACSQVDCRIMQK 60

Query: 61  GCPCFYPDNLINHASIAMNLYYNSKGKNQWNCDFRGSGLIVITDPSYGNCLYP 114
           GCPCFYPDNLINHASIAMNLYYNSKGKNQWNCDFRGSGLIVITDPSYGNCLYP
Sbjct: 61  GCPCFYPDNLINHASIAMNLYYNSKGKNQWNCDFRGSGLIVITDPSYGNCLYP 112

[Close]

BLAST of Carg14758 vs. NCBI nr
Match: XP_023525198.1 (major pollen allergen Ole e 10-like isoform X1 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 234.6 bits (597), Expect = 4.3e-58
Identity = 108/112 (96.43%), Postives = 109/112 (97.32%), Query Frame = 0


Query: 2   AKAAAVALSFLLLFLSGDLLAVNGYQKTWCVARPSSDHATLLANINYACSQVDCRIMQKG 61
           A A AV LSFLLLFLSGDLLAVNGYQKTWCVARPSSDHATLLANI+YACSQVDCRIMQKG
Sbjct: 4   AVAGAVTLSFLLLFLSGDLLAVNGYQKTWCVARPSSDHATLLANIDYACSQVDCRIMQKG 63

Query: 62  CPCFYPDNLINHASIAMNLYYNSKGKNQWNCDFRGSGLIVITDPSYGNCLYP 114
           CPCFYPDNLINHASIAMNLYYNSKGKNQWNCDFRGSGLIVITDPSYGNCLYP
Sbjct: 64  CPCFYPDNLINHASIAMNLYYNSKGKNQWNCDFRGSGLIVITDPSYGNCLYP 115

[Close]

BLAST of Carg14758 vs. ExPASy Swiss-Prot
Match: P52409 (Glucan endo-1,3-beta-glucosidase OS=Triticum aestivum OX=4565 GN=GLC1 PE=2 SV=1)

HSP 1 Score: 98.2 bits (243), Expect = 6.3e-20
Identity = 40/83 (48.19%), Postives = 53/83 (63.86%), Query Frame = 0


Query: 30  WCVARPSSDHATLLANINYACSQVDCRIMQKGCPCFYPDNLINHASIAMNLYYNSKGKNQ 89
           WCVA+  ++   L  NINYAC  VDC+ +Q G  CF P++L  HAS  MN YY + G   
Sbjct: 377 WCVAKDGANGTDLQNNINYACGFVDCKPIQSGGACFSPNSLQAHASYVMNAYYQANGHTD 436

Query: 90  WNCDFRGSGLIVITDPSYGNCLY 113
             CDF+G+G++  +DPSYG C Y
Sbjct: 437 LACDFKGTGIVTSSDPSYGGCKY 459

[Close]

BLAST of Carg14758 vs. ExPASy Swiss-Prot
Match: Q94G86 (Glucan endo-1,3-beta-D-glucosidase OS=Olea europaea OX=4146 GN=OLE9 PE=1 SV=1)

HSP 1 Score: 97.8 bits (242), Expect = 8.2e-20
Identity = 41/86 (47.67%), Postives = 53/86 (61.63%), Query Frame = 0


Query: 29  TWCVARPSSDHATLLANINYACSQ-VDCRIMQKGCPCFYPDNLINHASIAMNLYYNSKGK 88
           +WCV +P      L  NINYAC Q +DC  +Q G  CF P+ +  HA+  MNLYY S G+
Sbjct: 371 SWCVPKPGVSDDQLTGNINYACGQGIDCGPIQPGGACFEPNTVKAHAAYVMNLYYQSAGR 430

Query: 89  NQWNCDFRGSGLIVITDPSYGNCLYP 114
           N WNCDF  +  +  T+PSYG C +P
Sbjct: 431 NSWNCDFSQTATLTNTNPSYGACNFP 456

[Close]

BLAST of Carg14758 vs. ExPASy Swiss-Prot
Match: Q84V39 (Major pollen allergen Ole e 10 OS=Olea europaea OX=4146 PE=1 SV=1)

HSP 1 Score: 90.9 bits (224), Expect = 1.0e-17
Identity = 40/87 (45.98%), Postives = 56/87 (64.37%), Query Frame = 0


Query: 28  KTWCVARPSSDHATLLANINYACSQ--VDCRIMQKGCPCFYPDNLINHASIAMNLYYNSK 87
           K WCV +  +  A L +NI+Y CSQ  +DC  +Q    CF P+ +  HAS AMN +Y SK
Sbjct: 35  KKWCVPKAEATDAQLQSNIDYVCSQSGMDCGPIQANGACFNPNTVRAHASYAMNSWYQSK 94

Query: 88  GKNQWNCDFRGSGLIVITDPSYGNCLY 113
           G+N ++CDF G+G I  +DPS G+C +
Sbjct: 95  GRNDFDCDFSGTGAITSSDPSNGSCSF 121

[Close]

BLAST of Carg14758 vs. ExPASy Swiss-Prot
Match: O65399 (Glucan endo-1,3-beta-glucosidase 1 OS=Arabidopsis thaliana OX=3702 GN=At1g11820 PE=2 SV=3)

HSP 1 Score: 90.1 bits (222), Expect = 1.7e-17
Identity = 39/99 (39.39%), Postives = 61/99 (61.62%), Query Frame = 0


Query: 17  SGDLLAVNGYQKTWCVARPSSDHATLLANINYAC--SQVDCRIMQKGCPCFYPDNLINHA 76
           SG  LA +   +T+C+A    D  TL A +++AC   + +C  +Q G  C+ P+N+  HA
Sbjct: 368 SGTFLANDTTNQTYCIAMDGVDAKTLQAALDWACGPGRSNCSEIQPGESCYQPNNVKGHA 427

Query: 77  SIAMNLYYNSKGKNQWNCDFRGSGLIVITDPSYGNCLYP 114
           S A N YY  +G+   +CDF+G  +I  TDPS+G+C++P
Sbjct: 428 SFAFNSYYQKEGRASGSCDFKGVAMITTTDPSHGSCIFP 466

[Close]

BLAST of Carg14758 vs. ExPASy Swiss-Prot
Match: Q8VYE5 (Glucan endo-1,3-beta-glucosidase 12 OS=Arabidopsis thaliana OX=3702 GN=At4g29360 PE=2 SV=1)

HSP 1 Score: 88.2 bits (217), Expect = 6.5e-17
Identity = 38/90 (42.22%), Postives = 51/90 (56.67%), Query Frame = 0


Query: 25  GYQKTWCVARPSSDHATLLANINYAC--SQVDCRIMQKGCPCFYPDNLINHASIAMNLYY 84
           G  K WC+A   +    L   +++AC    VDC  +Q   PCF PD +++HAS A N YY
Sbjct: 386 GGTKKWCIASSQASVTELQTALDWACGPGNVDCSAVQPDQPCFEPDTVLSHASYAFNTYY 445

Query: 85  NSKGKNQWNCDFRGSGLIVITDPSYGNCLY 113
              G +  +C F G+ + V  DPSYGNCLY
Sbjct: 446 QQSGASSIDCSFNGASVEVDKDPSYGNCLY 475

[Close]

BLAST of Carg14758 vs. ExPASy TrEMBL
Match: A0A6J1FNL6 (glucan endo-1,3-beta-glucosidase-like isoform X1 OS=Cucurbita moschata OX=3662 GN=LOC111446803 PE=4 SV=1)

HSP 1 Score: 243.8 bits (621), Expect = 3.4e-61
Identity = 113/113 (100.00%), Postives = 113/113 (100.00%), Query Frame = 0


Query: 1   MAKAAAVALSFLLLFLSGDLLAVNGYQKTWCVARPSSDHATLLANINYACSQVDCRIMQK 60
           MAKAAAVALSFLLLFLSGDLLAVNGYQKTWCVARPSSDHATLLANINYACSQVDCRIMQK
Sbjct: 1   MAKAAAVALSFLLLFLSGDLLAVNGYQKTWCVARPSSDHATLLANINYACSQVDCRIMQK 60

Query: 61  GCPCFYPDNLINHASIAMNLYYNSKGKNQWNCDFRGSGLIVITDPSYGNCLYP 114
           GCPCFYPDNLINHASIAMNLYYNSKGKNQWNCDFRGSGLIVITDPSYGNCLYP
Sbjct: 61  GCPCFYPDNLINHASIAMNLYYNSKGKNQWNCDFRGSGLIVITDPSYGNCLYP 113

[Close]

BLAST of Carg14758 vs. ExPASy TrEMBL
Match: A0A6J1J1Y2 (major pollen allergen Ole e 10-like isoform X1 OS=Cucurbita maxima OX=3661 GN=LOC111481088 PE=4 SV=1)

HSP 1 Score: 238.4 bits (607), Expect = 1.4e-59
Identity = 110/113 (97.35%), Postives = 111/113 (98.23%), Query Frame = 0


Query: 1   MAKAAAVALSFLLLFLSGDLLAVNGYQKTWCVARPSSDHATLLANINYACSQVDCRIMQK 60
           MAKAAAV LSFLLLFLSGDLLAVNGYQKTWCV RPSSDHATLLANI+YACSQVDCRIMQK
Sbjct: 1   MAKAAAVTLSFLLLFLSGDLLAVNGYQKTWCVPRPSSDHATLLANIDYACSQVDCRIMQK 60

Query: 61  GCPCFYPDNLINHASIAMNLYYNSKGKNQWNCDFRGSGLIVITDPSYGNCLYP 114
           GCPCFYPDNLINHASIAMNLYYNSKGKNQWNCDFRGSGLIVITDPSYGNCLYP
Sbjct: 61  GCPCFYPDNLINHASIAMNLYYNSKGKNQWNCDFRGSGLIVITDPSYGNCLYP 113

[Close]

BLAST of Carg14758 vs. ExPASy TrEMBL
Match: A0A6J1FTX7 (major pollen allergen Ole e 10-like isoform X2 OS=Cucurbita moschata OX=3662 GN=LOC111446803 PE=4 SV=1)

HSP 1 Score: 236.9 bits (603), Expect = 4.2e-59
Identity = 112/113 (99.12%), Postives = 112/113 (99.12%), Query Frame = 0


Query: 1   MAKAAAVALSFLLLFLSGDLLAVNGYQKTWCVARPSSDHATLLANINYACSQVDCRIMQK 60
           MAKAAAVALSFLLLFLSGDLLAVNGYQ TWCVARPSSDHATLLANINYACSQVDCRIMQK
Sbjct: 1   MAKAAAVALSFLLLFLSGDLLAVNGYQ-TWCVARPSSDHATLLANINYACSQVDCRIMQK 60

Query: 61  GCPCFYPDNLINHASIAMNLYYNSKGKNQWNCDFRGSGLIVITDPSYGNCLYP 114
           GCPCFYPDNLINHASIAMNLYYNSKGKNQWNCDFRGSGLIVITDPSYGNCLYP
Sbjct: 61  GCPCFYPDNLINHASIAMNLYYNSKGKNQWNCDFRGSGLIVITDPSYGNCLYP 112

[Close]

BLAST of Carg14758 vs. ExPASy TrEMBL
Match: A0A6J1IYM5 (major pollen allergen Ole e 10-like isoform X2 OS=Cucurbita maxima OX=3661 GN=LOC111481088 PE=4 SV=1)

HSP 1 Score: 231.5 bits (589), Expect = 1.8e-57
Identity = 109/113 (96.46%), Postives = 110/113 (97.35%), Query Frame = 0


Query: 1   MAKAAAVALSFLLLFLSGDLLAVNGYQKTWCVARPSSDHATLLANINYACSQVDCRIMQK 60
           MAKAAAV LSFLLLFLSGDLLAVNGYQ TWCV RPSSDHATLLANI+YACSQVDCRIMQK
Sbjct: 1   MAKAAAVTLSFLLLFLSGDLLAVNGYQ-TWCVPRPSSDHATLLANIDYACSQVDCRIMQK 60

Query: 61  GCPCFYPDNLINHASIAMNLYYNSKGKNQWNCDFRGSGLIVITDPSYGNCLYP 114
           GCPCFYPDNLINHASIAMNLYYNSKGKNQWNCDFRGSGLIVITDPSYGNCLYP
Sbjct: 61  GCPCFYPDNLINHASIAMNLYYNSKGKNQWNCDFRGSGLIVITDPSYGNCLYP 112

[Close]

BLAST of Carg14758 vs. ExPASy TrEMBL
Match: A0A6J1CBF4 (glucan endo-1,3-beta-D-glucosidase OS=Momordica charantia OX=3673 GN=LOC111009159 PE=4 SV=1)

HSP 1 Score: 204.9 bits (520), Expect = 1.8e-49
Identity = 98/114 (85.96%), Postives = 103/114 (90.35%), Query Frame = 0


Query: 4   AAAVALSFLLLFLS----GDLLAVNGYQKTWCVARPSSDHATLLANINYACSQVDCRIMQ 63
           AAA AL F LLFLS    GDLLAVNG +KTWCVARPSSD ATLLANINYACSQVDCRIMQ
Sbjct: 2   AAAAALCFSLLFLSFLSEGDLLAVNG-EKTWCVARPSSDKATLLANINYACSQVDCRIMQ 61

Query: 64  KGCPCFYPDNLINHASIAMNLYYNSKGKNQWNCDFRGSGLIVITDPSYGNCLYP 114
           KGCPCFYPDNLIN AS+AMNLYY+S+GKNQWNCDFRGS LIVITDPSYGNC+YP
Sbjct: 62  KGCPCFYPDNLINGASVAMNLYYHSRGKNQWNCDFRGSALIVITDPSYGNCIYP 114

[Close]

BLAST of Carg14758 vs. TAIR 10
Match: AT1G78520.1 (Carbohydrate-binding X8 domain superfamily protein )

HSP 1 Score: 151.4 bits (381), Expect = 4.4e-37
Identity = 70/115 (60.87%), Postives = 84/115 (73.04%), Query Frame = 0


Query: 1   MAKAAAVALSFLL---LFLSGDLLAVNGYQKTWCVARPSSDHATLLANINYACSQVDCRI 60
           MAK A + LSFL+   L    + + VN   KTWCVA+PSSD   L  NIN+ACS VDCR+
Sbjct: 1   MAK-AWICLSFLIFLYLVSERNFINVNAETKTWCVAKPSSDQVALQDNINFACSHVDCRV 60

Query: 61  MQKGCPCFYPDNLINHASIAMNLYYNSKGKNQWNCDFRGSGLIVITDPSYGNCLY 113
           +  GCPC+ P NLINHASIAMNLYY + G+N WNC+F+ SGLI IT+PSYGNC Y
Sbjct: 61  LLSGCPCYSPSNLINHASIAMNLYYQANGRNYWNCNFKNSGLITITNPSYGNCYY 114

[Close]

BLAST of Carg14758 vs. TAIR 10
Match: AT2G43670.1 (Carbohydrate-binding X8 domain superfamily protein )

HSP 1 Score: 128.3 bits (321), Expect = 4.0e-30
Identity = 58/117 (49.57%), Postives = 85/117 (72.65%), Query Frame = 0


Query: 1   MAKA--AAVALSFLLLFLSGDLLAVNGYQKT---WCVARPSSDHATLLANINYACSQVDC 60
           MAKA      + FL L+  G+ + V    ++   WCVA+PS+D+  L  NIN+ACS++DC
Sbjct: 1   MAKAQICLCFIIFLYLWPEGNFIKVTKADRSAGDWCVAKPSTDNERLQENINFACSKIDC 60

Query: 61  RIMQKGCPCFYPDNLINHASIAMNLYYNSKGKNQWNCDFRGSGLIVITDPSYGNCLY 113
           +I+ +G  C+ PD++I+ AS+AMNLYY ++G++ WNC+F GSGLI ITDPSYG+C+Y
Sbjct: 61  QIISEGGACYLPDSIISRASVAMNLYYQAQGRHFWNCNFEGSGLIGITDPSYGSCIY 117

[Close]

BLAST of Carg14758 vs. TAIR 10
Match: AT4G09462.1 (Carbohydrate-binding X8 domain superfamily protein )

HSP 1 Score: 106.3 bits (264), Expect = 1.6e-23
Identity = 52/113 (46.02%), Postives = 70/113 (61.95%), Query Frame = 0


Query: 1   MAKAAAVALSFLLLFLSGDLLAVNGYQKTWCVARPSSDHATLLANINYACSQ-VDCRIMQ 60
           MAK ++  L+ L + LS  ++      KTWC+A   + +A L ANIN+ACSQ VDCR +Q
Sbjct: 1   MAKISS-PLALLFIMLSSIMINHVASSKTWCIATLIATNAQLQANINFACSQGVDCRPIQ 60

Query: 61  KGCPCFYPDNLINHASIAMNLYYNSKGKNQWNCDFRGSGLIVITDPSYGNCLY 113
            G  CF P+NL NHAS  MN YY + G+    C F+ +G    TDPS+G C+Y
Sbjct: 61  PGGSCFIPNNLANHASFVMNSYYQTHGRTNQACSFKNTGTFAATDPSFGKCVY 112

[Close]

BLAST of Carg14758 vs. TAIR 10
Match: AT3G28250.1 (Carbohydrate-binding X8 domain superfamily protein )

HSP 1 Score: 103.2 bits (256), Expect = 1.4e-22
Identity = 42/77 (54.55%), Postives = 56/77 (72.73%), Query Frame = 0


Query: 30  WCVARPSSDHATLLANINYACSQVDCRIMQKGCPCFYPDNLINHASIAMNLYYNSKGKNQ 89
           WCVA+P +    L+ N+NYACS VDC+I+     C+ PDN+ N AS+ MNLYY ++G+N 
Sbjct: 3   WCVAKPGTLTEQLINNLNYACSIVDCQIISTRGACYSPDNIYNMASVVMNLYYQAEGRNF 62

Query: 90  WNCDFRGSGLIVITDPS 107
           WNC+F  SGL+ ITDPS
Sbjct: 63  WNCNFGDSGLVAITDPS 79

[Close]

BLAST of Carg14758 vs. TAIR 10
Match: AT4G09464.1 (Carbohydrate-binding X8 domain superfamily protein )

HSP 1 Score: 102.4 bits (254), Expect = 2.4e-22
Identity = 49/113 (43.36%), Postives = 67/113 (59.29%), Query Frame = 0


Query: 5   AAVALSFLLLFLSGDLLAVN----GYQKTWCVARPSSDHATLLANINYACSQ-VDCRIMQ 64
           A ++    LLF+    + +N       KTWC+A   + +A L ANIN+ACSQ VDCR ++
Sbjct: 2   AKISSPLALLFIMLSSIMINHIHVASSKTWCIATLIATNAQLQANINFACSQGVDCRPIR 61

Query: 65  KGCPCFYPDNLINHASIAMNLYYNSKGKNQWNCDFRGSGLIVITDPSYGNCLY 113
            G  CF P+NL NHAS  MN YY + G+    C F+ +G    TDPS+G C+Y
Sbjct: 62  PGGSCFIPNNLANHASFVMNSYYQTHGRTNQACSFKNTGTFAATDPSFGKCVY 114

The following BLAST results are available for this feature:

BLAST of Carg14758 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_022941529.1	7.1e-61	100.00	glucan endo-1,3-beta-glucosidase-like isoform X1 [Cucurbita moschata] >KAG703746...	[more]
KAG6607951.1	1.2e-60	99.12	Glucan endo-1,3-beta-D-glucosidase, partial [Cucurbita argyrosperma subsp. soror...	[more]
XP_022982185.1	3.0e-59	97.35	major pollen allergen Ole e 10-like isoform X1 [Cucurbita maxima]	[more]
XP_022941530.1	8.6e-59	99.12	major pollen allergen Ole e 10-like isoform X2 [Cucurbita moschata]	[more]
XP_023525198.1	4.3e-58	96.43	major pollen allergen Ole e 10-like isoform X1 [Cucurbita pepo subsp. pepo]	[more]

BLAST of Carg14758 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
P52409	6.3e-20	48.19	Glucan endo-1,3-beta-glucosidase OS=Triticum aestivum OX=4565 GN=GLC1 PE=2 SV=1	[more]
Q94G86	8.2e-20	47.67	Glucan endo-1,3-beta-D-glucosidase OS=Olea europaea OX=4146 GN=OLE9 PE=1 SV=1	[more]
Q84V39	1.0e-17	45.98	Major pollen allergen Ole e 10 OS=Olea europaea OX=4146 PE=1 SV=1	[more]
O65399	1.7e-17	39.39	Glucan endo-1,3-beta-glucosidase 1 OS=Arabidopsis thaliana OX=3702 GN=At1g11820 ...	[more]
Q8VYE5	6.5e-17	42.22	Glucan endo-1,3-beta-glucosidase 12 OS=Arabidopsis thaliana OX=3702 GN=At4g29360...	[more]

BLAST of Carg14758 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1FNL6	3.4e-61	100.00	glucan endo-1,3-beta-glucosidase-like isoform X1 OS=Cucurbita moschata OX=3662 G...	[more]
A0A6J1J1Y2	1.4e-59	97.35	major pollen allergen Ole e 10-like isoform X1 OS=Cucurbita maxima OX=3661 GN=LO...	[more]
A0A6J1FTX7	4.2e-59	99.12	major pollen allergen Ole e 10-like isoform X2 OS=Cucurbita moschata OX=3662 GN=...	[more]
A0A6J1IYM5	1.8e-57	96.46	major pollen allergen Ole e 10-like isoform X2 OS=Cucurbita maxima OX=3661 GN=LO...	[more]
A0A6J1CBF4	1.8e-49	85.96	glucan endo-1,3-beta-D-glucosidase OS=Momordica charantia OX=3673 GN=LOC11100915...	[more]

BLAST of Carg14758 vs. TAIR 10


Match Name	E-value	Identity	Description
AT1G78520.1	4.4e-37	60.87	Carbohydrate-binding X8 domain superfamily protein	[more]
AT2G43670.1	4.0e-30	49.57	Carbohydrate-binding X8 domain superfamily protein	[more]
AT4G09462.1	1.6e-23	46.02	Carbohydrate-binding X8 domain superfamily protein	[more]
AT3G28250.1	1.4e-22	54.55	Carbohydrate-binding X8 domain superfamily protein	[more]
AT4G09464.1	2.4e-22	43.36	Carbohydrate-binding X8 domain superfamily protein	[more]

InterPro

Analysis Name: InterPro Annotations of Silver-seed gourd (SMH-JMG-627) v2
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR012946	X8 domain	SMART	SM00768	X8_cls	coord: 29..112 e-value: 5.1E-38 score: 142.3
IPR012946	X8 domain	PFAM	PF07983	X8	coord: 29..98 e-value: 3.5E-19 score: 69.3
None	No IPR available	GENE3D	1.20.58.1040		coord: 24..110 e-value: 2.0E-10 score: 42.9
None	No IPR available	PANTHER	PTHR31044:SF38	MAJOR POLLEN ALLERGEN OLE E 10-LIKE	coord: 9..113
IPR044788	Carbohydrate-binding X8 domain-containing protein, plant	PANTHER	PTHR31044	BETA-1,3 GLUCANASE	coord: 9..113

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Carg14758-RA	Carg14758-RA	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
cellular_component	GO:0009506	plasmodesma
molecular_function	GO:0016787	hydrolase activity