Carg14720 (gene) Silver-seed gourd (SMH-JMG-627) v2

Overview
NameCarg14720
Typegene
OrganismCucurbita argyrosperma subsp. argyrosperma cv. SMH-JMG-627 (Silver-seed gourd (SMH-JMG-627) v2)
Descriptionsodium/calcium exchanger NCL-like
LocationCarg_Chr01: 9937612 .. 9939010 (+)
RNA-Seq ExpressionCarg14720
SyntenyCarg14720
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptidethree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGAACATTCATGACAGGCTATTTTCAAGAATCGACGCGAATAAGAATGGACTTCTTTCAGCTTCTGAACTGAGAGCGTTAATCGTTGGCATTCAGTTCGAGGCGATTGACTTAGATCATGATGATGCTGTTAACAAAATATTGAGCGACTTCGATACTTCTCGTGATAACCATGTTGATTCTACTGAGTTTGCCAATGGGATCATTAGGTGGCTAGCTCAAGTACAAGGAGCAAGAACTGGCAGAGCTGATGATGGTCCACACACATTGAAATACCTGCATAACTTTCATCAAGTAAGTTGTAACCTGGTCGGCGGTCGCTGTAATTTTCCTCTTGATTCTTACCGTCGCGTTTCTGAAGCATGACATGATGTATATACAGGAAACAAAGAGAGAGCATGATTTGCTGGACGTTGGGGAACAGAACGACGAGGTCGTAGAGGGTGTCGAGGAGAGCAAGGGAGTGTTGATCAGAGCCATACTCTTCTTGTTGCTTGGAACAGCCATTGCTGCTGCCTTCGCTGATCCTTTGGTCGACGTTGTACATAACTTCTCCAATGCAACGAGTATCCCTGCGTTCTTCATTTCGTTTATCGCTTTGCCTTTAGCAACCAACTCCAGTGAAGCAGTCTCTGCTATAATCTTTGCAAGCCGTGACAAGCGAAAAACCGCCTCCTTAACATTTTCTGAGGTTTGTTCTTTCTTATATACTTAATATTGAACTGTGCTGTGTGGAGAAGAATGATTGATATCTAAGCTTCCTTGGTGCAGTTATATGGAGCAGTGACAATGAACAATGTGCTTTGTCTATCTGTGTTCTTGGCTCTTGTTTATCTGAGGGGATTGGTTTGGAACTTTTCTTCTGAAGTATTGGTTATTCTGGTCGTTACGATGATCATGGGACTGCTCGGGAGCTTCCGAACCGCGTTCCCGCTGTGGACAGCCCTGGTGGCTCTCCTTCTCTACCCCCTGTCTCTTGTGCTGGTCTATGTCCTTGATTATGTGTTTGGTTGGTCATAGATGAGTTGGTGTGTTTGAGTTATATATGTTCACAGGAGGTTTGTTGATCTAAATTTCCGGATGTTTTAGACACACTCTGCTGAGGCTGTGTTAGAGAAACTCCACTGTTCTGCTCTCTCTTTTATTTGCTTTATTGTTCCTACTTTTGTCGATATATTTTGTTTTGGTACGACCGACACCTTTGATCACGGTGATACATAACAGGTCAAAATAGACAAATATCTATTAGCGGTGAACTAAAATTGTGAGGCTGACGACAATATCTACTAGAGTGAGTTTGGGTTGTTACAAATAGTATCAGAACTAGACACCAAGTGATGTGCTAGCGAAGACACTAACCCCAAGGGGTAGATTGTGAGATTAGATCTCACGTTGATTG

mRNA sequence

ATGAACATTCATGACAGGCTATTTTCAAGAATCGACGCGAATAAGAATGGACTTCTTTCAGCTTCTGAACTGAGAGCGTTAATCGTTGGCATTCAGTTCGAGGCGATTGACTTAGATCATGATGATGCTGTTAACAAAATATTGAGCGACTTCGATACTTCTCGTGATAACCATGTTGATTCTACTGAGTTTGCCAATGGGATCATTAGGTGGCTAGCTCAAGTACAAGGAGCAAGAACTGGCAGAGCTGATGATGGTCCACACACATTGAAATACCTGCATAACTTTCATCAAGAAACAAAGAGAGAGCATGATTTGCTGGACGTTGGGGAACAGAACGACGAGGTCGTAGAGGGTGTCGAGGAGAGCAAGGGAGTGTTGATCAGAGCCATACTCTTCTTGTTGCTTGGAACAGCCATTGCTGCTGCCTTCGCTGATCCTTTGGTCGACGTTGTACATAACTTCTCCAATGCAACGAGTATCCCTGCGTTCTTCATTTCGTTTATCGCTTTGCCTTTAGCAACCAACTCCAGTGAAGCAGTCTCTGCTATAATCTTTGCAAGCCGTGACAAGCGAAAAACCGCCTCCTTAACATTTTCTGAGTTATATGGAGCAGTGACAATGAACAATGTGCTTTGTCTATCTGTGTTCTTGGCTCTTGTTTATCTGAGGGGATTGGTTTGGAACTTTTCTTCTGAAGTATTGGTTATTCTGGTCGTTACGATGATCATGGGACTGCTCGGGAGCTTCCGAACCGCGTTCCCGCTGTGGACAGCCCTGGTGGCTCTCCTTCTCTACCCCCTGTCTCTTGTGCTGGTCTATGTCCTTGATTATGTGTTTGGTTGGTCATAGATGAGTTGGTGTGTTTGAGTTATATATGTTCACAGGAGGTTTGTTGATCTAAATTTCCGGATGTTTTAGACACACTCTGCTGAGGCTGTGTTAGAGAAACTCCACTGTTCTGCTCTCTCTTTTATTTGCTTTATTGTTCCTACTTTTGTCGATATATTTTGTTTTGGTACGACCGACACCTTTGATCACGGTGATACATAACAGGTCAAAATAGACAAATATCTATTAGCGGTGAACTAAAATTGTGAGGCTGACGACAATATCTACTAGAGTGAGTTTGGGTTGTTACAAATAGTATCAGAACTAGACACCAAGTGATGTGCTAGCGAAGACACTAACCCCAAGGGGTAGATTGTGAGATTAGATCTCACGTTGATTG

Coding sequence (CDS)

ATGAACATTCATGACAGGCTATTTTCAAGAATCGACGCGAATAAGAATGGACTTCTTTCAGCTTCTGAACTGAGAGCGTTAATCGTTGGCATTCAGTTCGAGGCGATTGACTTAGATCATGATGATGCTGTTAACAAAATATTGAGCGACTTCGATACTTCTCGTGATAACCATGTTGATTCTACTGAGTTTGCCAATGGGATCATTAGGTGGCTAGCTCAAGTACAAGGAGCAAGAACTGGCAGAGCTGATGATGGTCCACACACATTGAAATACCTGCATAACTTTCATCAAGAAACAAAGAGAGAGCATGATTTGCTGGACGTTGGGGAACAGAACGACGAGGTCGTAGAGGGTGTCGAGGAGAGCAAGGGAGTGTTGATCAGAGCCATACTCTTCTTGTTGCTTGGAACAGCCATTGCTGCTGCCTTCGCTGATCCTTTGGTCGACGTTGTACATAACTTCTCCAATGCAACGAGTATCCCTGCGTTCTTCATTTCGTTTATCGCTTTGCCTTTAGCAACCAACTCCAGTGAAGCAGTCTCTGCTATAATCTTTGCAAGCCGTGACAAGCGAAAAACCGCCTCCTTAACATTTTCTGAGTTATATGGAGCAGTGACAATGAACAATGTGCTTTGTCTATCTGTGTTCTTGGCTCTTGTTTATCTGAGGGGATTGGTTTGGAACTTTTCTTCTGAAGTATTGGTTATTCTGGTCGTTACGATGATCATGGGACTGCTCGGGAGCTTCCGAACCGCGTTCCCGCTGTGGACAGCCCTGGTGGCTCTCCTTCTCTACCCCCTGTCTCTTGTGCTGGTCTATGTCCTTGATTATGTGTTTGGTTGGTCATAG

Protein sequence

MNIHDRLFSRIDANKNGLLSASELRALIVGIQFEAIDLDHDDAVNKILSDFDTSRDNHVDSTEFANGIIRWLAQVQGARTGRADDGPHTLKYLHNFHQETKREHDLLDVGEQNDEVVEGVEESKGVLIRAILFLLLGTAIAAAFADPLVDVVHNFSNATSIPAFFISFIALPLATNSSEAVSAIIFASRDKRKTASLTFSELYGAVTMNNVLCLSVFLALVYLRGLVWNFSSEVLVILVVTMIMGLLGSFRTAFPLWTALVALLLYPLSLVLVYVLDYVFGWS
Homology
BLAST of Carg14720 vs. NCBI nr
Match: KAG7037430.1 (Sodium/calcium exchanger NCL1, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 532.7 bits (1371), Expect = 1.9e-147
Identity = 283/283 (100.00%), Postives = 283/283 (100.00%), Query Frame = 0

Query: 1   MNIHDRLFSRIDANKNGLLSASELRALIVGIQFEAIDLDHDDAVNKILSDFDTSRDNHVD 60
           MNIHDRLFSRIDANKNGLLSASELRALIVGIQFEAIDLDHDDAVNKILSDFDTSRDNHVD
Sbjct: 1   MNIHDRLFSRIDANKNGLLSASELRALIVGIQFEAIDLDHDDAVNKILSDFDTSRDNHVD 60

Query: 61  STEFANGIIRWLAQVQGARTGRADDGPHTLKYLHNFHQETKREHDLLDVGEQNDEVVEGV 120
           STEFANGIIRWLAQVQGARTGRADDGPHTLKYLHNFHQETKREHDLLDVGEQNDEVVEGV
Sbjct: 61  STEFANGIIRWLAQVQGARTGRADDGPHTLKYLHNFHQETKREHDLLDVGEQNDEVVEGV 120

Query: 121 EESKGVLIRAILFLLLGTAIAAAFADPLVDVVHNFSNATSIPAFFISFIALPLATNSSEA 180
           EESKGVLIRAILFLLLGTAIAAAFADPLVDVVHNFSNATSIPAFFISFIALPLATNSSEA
Sbjct: 121 EESKGVLIRAILFLLLGTAIAAAFADPLVDVVHNFSNATSIPAFFISFIALPLATNSSEA 180

Query: 181 VSAIIFASRDKRKTASLTFSELYGAVTMNNVLCLSVFLALVYLRGLVWNFSSEVLVILVV 240
           VSAIIFASRDKRKTASLTFSELYGAVTMNNVLCLSVFLALVYLRGLVWNFSSEVLVILVV
Sbjct: 181 VSAIIFASRDKRKTASLTFSELYGAVTMNNVLCLSVFLALVYLRGLVWNFSSEVLVILVV 240

Query: 241 TMIMGLLGSFRTAFPLWTALVALLLYPLSLVLVYVLDYVFGWS 284
           TMIMGLLGSFRTAFPLWTALVALLLYPLSLVLVYVLDYVFGWS
Sbjct: 241 TMIMGLLGSFRTAFPLWTALVALLLYPLSLVLVYVLDYVFGWS 283

BLAST of Carg14720 vs. NCBI nr
Match: KAG6607902.1 (Sodium/calcium exchanger NCL, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 521.5 bits (1342), Expect = 4.4e-144
Identity = 278/281 (98.93%), Postives = 280/281 (99.64%), Query Frame = 0

Query: 3   IHDRLFSRIDANKNGLLSASELRALIVGIQFEAIDLDHDDAVNKILSDFDTSRDNHVDST 62
           I ++LFSRIDANKNGLLSASELRALIVGIQFEAIDLDHDDAVNKILSDFDTSRDNHVDST
Sbjct: 301 IIEKLFSRIDANKNGLLSASELRALIVGIQFEAIDLDHDDAVNKILSDFDTSRDNHVDST 360

Query: 63  EFANGIIRWLAQVQGARTGRADDGPHTLKYLHNFHQETKREHDLLDVGEQNDEVVEGVEE 122
           EFANGIIRWLAQVQGARTGRADDGPHTLKYLHNFHQETKREHDLLDVGEQNDEVVEGVEE
Sbjct: 361 EFANGIIRWLAQVQGARTGRADDGPHTLKYLHNFHQETKREHDLLDVGEQNDEVVEGVEE 420

Query: 123 SKGVLIRAILFLLLGTAIAAAFADPLVDVVHNFSNATSIPAFFISFIALPLATNSSEAVS 182
           SKGVLIRAILFLLLGTAIAAAFADPLVDVVHNFSNATSIPAFFISFIALPLATNSSEAVS
Sbjct: 421 SKGVLIRAILFLLLGTAIAAAFADPLVDVVHNFSNATSIPAFFISFIALPLATNSSEAVS 480

Query: 183 AIIFASRDKRKTASLTFSELYGAVTMNNVLCLSVFLALVYLRGLVWNFSSEVLVILVVTM 242
           AIIFASRDKRKTASLTFSELYGAVTMNNVLCLSVFLALVYLRGLVWNFSSEVLVILVVTM
Sbjct: 481 AIIFASRDKRKTASLTFSELYGAVTMNNVLCLSVFLALVYLRGLVWNFSSEVLVILVVTM 540

Query: 243 IMGLLGSFRTAFPLWTALVALLLYPLSLVLVYVLDYVFGWS 284
           IMGLLGSFRTAFPLWTALVALLLYPLSLVLVYVLDYVFGWS
Sbjct: 541 IMGLLGSFRTAFPLWTALVALLLYPLSLVLVYVLDYVFGWS 581

BLAST of Carg14720 vs. NCBI nr
Match: XP_022941280.1 (sodium/calcium exchanger NCL-like [Cucurbita moschata])

HSP 1 Score: 519.6 bits (1337), Expect = 1.7e-143
Identity = 277/281 (98.58%), Postives = 280/281 (99.64%), Query Frame = 0

Query: 3   IHDRLFSRIDANKNGLLSASELRALIVGIQFEAIDLDHDDAVNKILSDFDTSRDNHVDST 62
           I ++LFSRIDANKNGLLSASELRALIVGIQFEAIDLDHDDAVNKILSDFDTSRDNHVDST
Sbjct: 301 IIEKLFSRIDANKNGLLSASELRALIVGIQFEAIDLDHDDAVNKILSDFDTSRDNHVDST 360

Query: 63  EFANGIIRWLAQVQGARTGRADDGPHTLKYLHNFHQETKREHDLLDVGEQNDEVVEGVEE 122
           EFANGIIRWLAQVQGARTGRADDGPHTLKYLHNFHQETKREHDLLDVGEQ+DEVVEGVEE
Sbjct: 361 EFANGIIRWLAQVQGARTGRADDGPHTLKYLHNFHQETKREHDLLDVGEQSDEVVEGVEE 420

Query: 123 SKGVLIRAILFLLLGTAIAAAFADPLVDVVHNFSNATSIPAFFISFIALPLATNSSEAVS 182
           SKGVLIRAILFLLLGTAIAAAFADPLVDVVHNFSNATSIPAFFISFIALPLATNSSEAVS
Sbjct: 421 SKGVLIRAILFLLLGTAIAAAFADPLVDVVHNFSNATSIPAFFISFIALPLATNSSEAVS 480

Query: 183 AIIFASRDKRKTASLTFSELYGAVTMNNVLCLSVFLALVYLRGLVWNFSSEVLVILVVTM 242
           AIIFASRDKRKTASLTFSELYGAVTMNNVLCLSVFLALVYLRGLVWNFSSEVLVILVVTM
Sbjct: 481 AIIFASRDKRKTASLTFSELYGAVTMNNVLCLSVFLALVYLRGLVWNFSSEVLVILVVTM 540

Query: 243 IMGLLGSFRTAFPLWTALVALLLYPLSLVLVYVLDYVFGWS 284
           IMGLLGSFRTAFPLWTALVALLLYPLSLVLVYVLDYVFGWS
Sbjct: 541 IMGLLGSFRTAFPLWTALVALLLYPLSLVLVYVLDYVFGWS 581

BLAST of Carg14720 vs. NCBI nr
Match: XP_022981259.1 (sodium/calcium exchanger NCL-like [Cucurbita maxima])

HSP 1 Score: 516.2 bits (1328), Expect = 1.8e-142
Identity = 275/278 (98.92%), Postives = 277/278 (99.64%), Query Frame = 0

Query: 6   RLFSRIDANKNGLLSASELRALIVGIQFEAIDLDHDDAVNKILSDFDTSRDNHVDSTEFA 65
           +LFSRIDANKNGLLSASELRALIVGIQFEAIDLDHDDAVNKILSDFDTSRDNHVDS EFA
Sbjct: 302 KLFSRIDANKNGLLSASELRALIVGIQFEAIDLDHDDAVNKILSDFDTSRDNHVDSIEFA 361

Query: 66  NGIIRWLAQVQGARTGRADDGPHTLKYLHNFHQETKREHDLLDVGEQNDEVVEGVEESKG 125
           NGIIRWLAQVQGARTGRADDGPHTLKYLHNFHQETKREHDLLDVGEQ+DEVVEGVEESKG
Sbjct: 362 NGIIRWLAQVQGARTGRADDGPHTLKYLHNFHQETKREHDLLDVGEQSDEVVEGVEESKG 421

Query: 126 VLIRAILFLLLGTAIAAAFADPLVDVVHNFSNATSIPAFFISFIALPLATNSSEAVSAII 185
           VLIRAILFLLLGTAIAAAFADPLVDVVHNFSNATSIPAFFISFIALPLATNSSEAVSAII
Sbjct: 422 VLIRAILFLLLGTAIAAAFADPLVDVVHNFSNATSIPAFFISFIALPLATNSSEAVSAII 481

Query: 186 FASRDKRKTASLTFSELYGAVTMNNVLCLSVFLALVYLRGLVWNFSSEVLVILVVTMIMG 245
           FASRDKRKTASLTFSELYGAVTMNNVLCLSVFLALVYLRGLVWNFSSEVLVILVVTMIMG
Sbjct: 482 FASRDKRKTASLTFSELYGAVTMNNVLCLSVFLALVYLRGLVWNFSSEVLVILVVTMIMG 541

Query: 246 LLGSFRTAFPLWTALVALLLYPLSLVLVYVLDYVFGWS 284
           LLGSFRTAFPLWTALVALLLYPLSLVLVYVLDYVFGWS
Sbjct: 542 LLGSFRTAFPLWTALVALLLYPLSLVLVYVLDYVFGWS 579

BLAST of Carg14720 vs. NCBI nr
Match: XP_023523899.1 (sodium/calcium exchanger NCL-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 515.4 bits (1326), Expect = 3.1e-142
Identity = 274/281 (97.51%), Postives = 279/281 (99.29%), Query Frame = 0

Query: 3   IHDRLFSRIDANKNGLLSASELRALIVGIQFEAIDLDHDDAVNKILSDFDTSRDNHVDST 62
           I D+LFSRIDANKNGLLSASELRALIVGIQFEAIDLDHDDAVNKILSDFDTSRD+HVD+T
Sbjct: 301 IIDKLFSRIDANKNGLLSASELRALIVGIQFEAIDLDHDDAVNKILSDFDTSRDSHVDAT 360

Query: 63  EFANGIIRWLAQVQGARTGRADDGPHTLKYLHNFHQETKREHDLLDVGEQNDEVVEGVEE 122
           EFANGIIRWLA VQGARTGRADDGPHT+KYLHNFHQETKREHDLLDVGEQ+DEVVEGVEE
Sbjct: 361 EFANGIIRWLAHVQGARTGRADDGPHTMKYLHNFHQETKREHDLLDVGEQSDEVVEGVEE 420

Query: 123 SKGVLIRAILFLLLGTAIAAAFADPLVDVVHNFSNATSIPAFFISFIALPLATNSSEAVS 182
           SKGVLIRAILFLLLGTAIAAAFADPLVDVVHNFSNATSIPAFFISFIALPLATNSSEAVS
Sbjct: 421 SKGVLIRAILFLLLGTAIAAAFADPLVDVVHNFSNATSIPAFFISFIALPLATNSSEAVS 480

Query: 183 AIIFASRDKRKTASLTFSELYGAVTMNNVLCLSVFLALVYLRGLVWNFSSEVLVILVVTM 242
           AIIFASRDKRKTASLTFSELYGAVTMNNVLCLSVFLALVYLRGLVWNFSSEVLVILVVTM
Sbjct: 481 AIIFASRDKRKTASLTFSELYGAVTMNNVLCLSVFLALVYLRGLVWNFSSEVLVILVVTM 540

Query: 243 IMGLLGSFRTAFPLWTALVALLLYPLSLVLVYVLDYVFGWS 284
           IMGLLGSFRTAFPLWTALVALLLYPLSLVLVYVLDYVFGWS
Sbjct: 541 IMGLLGSFRTAFPLWTALVALLLYPLSLVLVYVLDYVFGWS 581

BLAST of Carg14720 vs. ExPASy Swiss-Prot
Match: Q8L636 (Sodium/calcium exchanger NCL OS=Arabidopsis thaliana OX=3702 GN=NCL PE=2 SV=1)

HSP 1 Score: 335.5 bits (859), Expect = 5.8e-91
Identity = 184/280 (65.71%), Postives = 212/280 (75.71%), Query Frame = 0

Query: 6   RLFSRIDANKNGLLSASELRALIVGIQFEAIDLDHDDAVNKILSDFDTSRDNHVDSTEFA 65
           +LF  IDAN +G LSA+EL+ALI+GI FE ID D DDAV K+L DFD + D  VD  EF 
Sbjct: 306 KLFLTIDANNDGHLSAAELKALIIGISFEDIDFDKDDAVGKVLQDFDKTLDEQVDQEEFV 365

Query: 66  NGIIRWLAQVQGARTGRADDGPHTLKYLHNFHQETKREHDLLDVGE--QNDEVVEGVEES 125
            GI +WL Q  G      + GP T+K+L NFH +TKREH LL   E  +NDE    V + 
Sbjct: 366 RGIKQWLIQAMGGAPSGPEAGPRTMKFLDNFHVQTKREHALLGDNENGENDEEGGEVADP 425

Query: 126 KGVLIRAILFLLLGTAIAAAFADPLVDVVHNFSNATSIPAFFISFIALPLATNSSEAVSA 185
           K + I+A L LLLG AIAAAFADPLVD V+NFS AT IP+FFISFIALPLATNSSEAVSA
Sbjct: 426 KWITIKAALLLLLGAAIAAAFADPLVDTVNNFSAATGIPSFFISFIALPLATNSSEAVSA 485

Query: 186 IIFASRDKRKTASLTFSELYGAVTMNNVLCLSVFLALVYLRGLVWNFSSEVLVILVVTMI 245
           IIFASR K +TASLTFSEL G VTMNN+LCLSVFLA+VY+RGL WNFSSEVLVIL+V ++
Sbjct: 486 IIFASRKKIRTASLTFSELCGGVTMNNILCLSVFLAIVYVRGLTWNFSSEVLVILIVCLV 545

Query: 246 MGLLGSFRTAFPLWTALVALLLYPLSLVLVYVLDYVFGWS 284
           MG   SFRT +PLWT  +A LLYP SL LVY+LDY FGWS
Sbjct: 546 MGGFASFRTTYPLWTCFIAYLLYPFSLGLVYILDYWFGWS 585

BLAST of Carg14720 vs. ExPASy Swiss-Prot
Match: Q5QNI2 (Sodium/calcium exchanger NCL1 OS=Oryza sativa subsp. japonica OX=39947 GN=NCL1 PE=2 SV=1)

HSP 1 Score: 315.5 bits (807), Expect = 6.3e-85
Identity = 170/278 (61.15%), Postives = 218/278 (78.42%), Query Frame = 0

Query: 6   RLFSRIDANKNGLLSASELRALIVGIQFEAIDLDHDDAVNKILSDFDTSRDNHVDSTEFA 65
           +LF +ID +++  LS +EL ALI+GI FE +D D +DAV+KI+ DFDTS ++ V+  EF 
Sbjct: 312 KLFHKIDMDESQTLSRAELHALIIGINFEEVDFDKNDAVDKIMDDFDTSGNDIVEEAEFV 371

Query: 66  NGIIRWLAQVQGARTGRADDGPHTLKYLHNFHQETKREHDLLDVGEQNDEVVEGVEESKG 125
           +G+ RWL +   A+      G ++ K++ ++H  T++EHDLL   +++DE VE VE    
Sbjct: 372 SGMKRWLNE---AKRSVPTSGAYSNKFITDYHARTRQEHDLL--VDRSDETVESVENPGW 431

Query: 126 VLIRAILFLLLGTAIAAAFADPLVDVVHNFSNATSIPAFFISFIALPLATNSSEAVSAII 185
            + +A+  LLLG+AIAAAFADPLVD VHNFSNA+ IP+FFISFIALPLATNSSEAVSAII
Sbjct: 432 CITKAVGLLLLGSAIAAAFADPLVDAVHNFSNASHIPSFFISFIALPLATNSSEAVSAII 491

Query: 186 FASRDKRKTASLTFSELYGAVTMNNVLCLSVFLALVYLRGLVWNFSSEVLVILVVTMIMG 245
           FASR K +T+SLTFSE+YG VTMNN LCL VFLAL+Y+R L W+FSSEVL+IL+V +IMG
Sbjct: 492 FASRKKLRTSSLTFSEVYGGVTMNNTLCLGVFLALIYIRNLTWDFSSEVLIILLVCVIMG 551

Query: 246 LLGSFRTAFPLWTALVALLLYPLSLVLVYVLDYVFGWS 284
           L  SFRT FPLWT LVA +LYPLSLV+VY+LD+VFGWS
Sbjct: 552 LFTSFRTTFPLWTCLVAYMLYPLSLVVVYILDFVFGWS 584

BLAST of Carg14720 vs. ExPASy Swiss-Prot
Match: Q6K3R5 (Sodium/calcium exchanger NCL2 OS=Oryza sativa subsp. japonica OX=39947 GN=NCL2 PE=2 SV=2)

HSP 1 Score: 302.0 bits (772), Expect = 7.2e-81
Identity = 168/284 (59.15%), Postives = 215/284 (75.70%), Query Frame = 0

Query: 1   MNIHDRLFSRIDANKNGLLSASELRALIVGIQFEAIDLDHDDAVNKILSDFDTSRDNHVD 60
           +++ ++LF RID + +G L   EL+A IVGI FE ID + + A +++++DFDTSR++ ++
Sbjct: 299 VSVIEKLFHRIDQDNDGKLERGELQAFIVGINFEDIDWNSNLAADQVMADFDTSRNHFIE 358

Query: 61  STEFANGIIRWLAQVQGARTGRADDGPHTLKYLHNFHQETKREH-DLLDVGEQNDEVVEG 120
             EF NG++RWL + +   T     G ++ K+L++FH  T+ E   LLD  E+  E  +G
Sbjct: 359 KGEFVNGMLRWLDEAKRTVT----SGAYSKKFLNDFHARTRDEQTGLLDKDEEEGE-ADG 418

Query: 121 VEESKGVLIRAILFLLLGTAIAAAFADPLVDVVHNFSNATSIPAFFISFIALPLATNSSE 180
                   I+AIL LLLGTA+AAA ADPLVD VHNFSNAT IP+FFISFI +PLATNSSE
Sbjct: 419 --NPTWTCIKAILLLLLGTAMAAASADPLVDAVHNFSNATHIPSFFISFIVMPLATNSSE 478

Query: 181 AVSAIIFASRDKRKTASLTFSELYGAVTMNNVLCLSVFLALVYLRGLVWNFSSEVLVILV 240
           AVSAIIFASR K++T SLTFSE+YG VTMNN LCL+VFLALVY+RGL W+FSSEVL+IL+
Sbjct: 479 AVSAIIFASRKKKRTLSLTFSEVYGGVTMNNTLCLAVFLALVYVRGLTWDFSSEVLIILL 538

Query: 241 VTMIMGLLGSFRTAFPLWTALVALLLYPLSLVLVYVLDYVFGWS 284
           V +IMGL  SFRT FPLWT  VA LLYPLSL++VY+LDY FGWS
Sbjct: 539 VCIIMGLFTSFRTDFPLWTCFVAFLLYPLSLIMVYILDYKFGWS 575

BLAST of Carg14720 vs. ExPASy Swiss-Prot
Match: Q5KQN0 (Vacuolar cation/proton exchanger 2 OS=Oryza sativa subsp. japonica OX=39947 GN=CAX2 PE=2 SV=2)

HSP 1 Score: 47.0 bits (110), Expect = 4.2e-04
Identity = 41/139 (29.50%), Postives = 71/139 (51.08%), Query Frame = 0

Query: 109 VGEQNDEVVEGVEESKGVLI-RAILFLLLGTAIAAAFADPLVDVVHNFSNATSIPAFFIS 168
           +GEQ +EV E  EE K +    AI +L + T   +  +  LVD +   S + ++P  FIS
Sbjct: 261 IGEQEEEVTEDEEEEKEITQGEAICWLFVLTIWISILSGYLVDAIQGASESLNMPVAFIS 320

Query: 169 FIALPLATNSSEAVSAIIFASRDKRKTASLTFSELYGAVTMNNVLCLSVFLALVYLRG-- 228
            I LP+  N++E  SAI+FA +DK     +T     G+ T  ++  +   + + ++ G  
Sbjct: 321 VILLPIVGNAAEHASAIMFAMKDK---LDITLGVAIGSSTQISMFVIPFCVVIGWIMGQQ 380

Query: 229 LVWNFSSEVLVILVVTMIM 245
           +  NF       L +T+++
Sbjct: 381 MDLNFQLFETATLFITVLV 396

BLAST of Carg14720 vs. ExPASy TrEMBL
Match: A0A6J1FT50 (sodium/calcium exchanger NCL-like OS=Cucurbita moschata OX=3662 GN=LOC111446626 PE=4 SV=1)

HSP 1 Score: 519.6 bits (1337), Expect = 8.1e-144
Identity = 277/281 (98.58%), Postives = 280/281 (99.64%), Query Frame = 0

Query: 3   IHDRLFSRIDANKNGLLSASELRALIVGIQFEAIDLDHDDAVNKILSDFDTSRDNHVDST 62
           I ++LFSRIDANKNGLLSASELRALIVGIQFEAIDLDHDDAVNKILSDFDTSRDNHVDST
Sbjct: 301 IIEKLFSRIDANKNGLLSASELRALIVGIQFEAIDLDHDDAVNKILSDFDTSRDNHVDST 360

Query: 63  EFANGIIRWLAQVQGARTGRADDGPHTLKYLHNFHQETKREHDLLDVGEQNDEVVEGVEE 122
           EFANGIIRWLAQVQGARTGRADDGPHTLKYLHNFHQETKREHDLLDVGEQ+DEVVEGVEE
Sbjct: 361 EFANGIIRWLAQVQGARTGRADDGPHTLKYLHNFHQETKREHDLLDVGEQSDEVVEGVEE 420

Query: 123 SKGVLIRAILFLLLGTAIAAAFADPLVDVVHNFSNATSIPAFFISFIALPLATNSSEAVS 182
           SKGVLIRAILFLLLGTAIAAAFADPLVDVVHNFSNATSIPAFFISFIALPLATNSSEAVS
Sbjct: 421 SKGVLIRAILFLLLGTAIAAAFADPLVDVVHNFSNATSIPAFFISFIALPLATNSSEAVS 480

Query: 183 AIIFASRDKRKTASLTFSELYGAVTMNNVLCLSVFLALVYLRGLVWNFSSEVLVILVVTM 242
           AIIFASRDKRKTASLTFSELYGAVTMNNVLCLSVFLALVYLRGLVWNFSSEVLVILVVTM
Sbjct: 481 AIIFASRDKRKTASLTFSELYGAVTMNNVLCLSVFLALVYLRGLVWNFSSEVLVILVVTM 540

Query: 243 IMGLLGSFRTAFPLWTALVALLLYPLSLVLVYVLDYVFGWS 284
           IMGLLGSFRTAFPLWTALVALLLYPLSLVLVYVLDYVFGWS
Sbjct: 541 IMGLLGSFRTAFPLWTALVALLLYPLSLVLVYVLDYVFGWS 581

BLAST of Carg14720 vs. ExPASy TrEMBL
Match: A0A6J1J1E5 (sodium/calcium exchanger NCL-like OS=Cucurbita maxima OX=3661 GN=LOC111480448 PE=4 SV=1)

HSP 1 Score: 516.2 bits (1328), Expect = 8.9e-143
Identity = 275/278 (98.92%), Postives = 277/278 (99.64%), Query Frame = 0

Query: 6   RLFSRIDANKNGLLSASELRALIVGIQFEAIDLDHDDAVNKILSDFDTSRDNHVDSTEFA 65
           +LFSRIDANKNGLLSASELRALIVGIQFEAIDLDHDDAVNKILSDFDTSRDNHVDS EFA
Sbjct: 302 KLFSRIDANKNGLLSASELRALIVGIQFEAIDLDHDDAVNKILSDFDTSRDNHVDSIEFA 361

Query: 66  NGIIRWLAQVQGARTGRADDGPHTLKYLHNFHQETKREHDLLDVGEQNDEVVEGVEESKG 125
           NGIIRWLAQVQGARTGRADDGPHTLKYLHNFHQETKREHDLLDVGEQ+DEVVEGVEESKG
Sbjct: 362 NGIIRWLAQVQGARTGRADDGPHTLKYLHNFHQETKREHDLLDVGEQSDEVVEGVEESKG 421

Query: 126 VLIRAILFLLLGTAIAAAFADPLVDVVHNFSNATSIPAFFISFIALPLATNSSEAVSAII 185
           VLIRAILFLLLGTAIAAAFADPLVDVVHNFSNATSIPAFFISFIALPLATNSSEAVSAII
Sbjct: 422 VLIRAILFLLLGTAIAAAFADPLVDVVHNFSNATSIPAFFISFIALPLATNSSEAVSAII 481

Query: 186 FASRDKRKTASLTFSELYGAVTMNNVLCLSVFLALVYLRGLVWNFSSEVLVILVVTMIMG 245
           FASRDKRKTASLTFSELYGAVTMNNVLCLSVFLALVYLRGLVWNFSSEVLVILVVTMIMG
Sbjct: 482 FASRDKRKTASLTFSELYGAVTMNNVLCLSVFLALVYLRGLVWNFSSEVLVILVVTMIMG 541

Query: 246 LLGSFRTAFPLWTALVALLLYPLSLVLVYVLDYVFGWS 284
           LLGSFRTAFPLWTALVALLLYPLSLVLVYVLDYVFGWS
Sbjct: 542 LLGSFRTAFPLWTALVALLLYPLSLVLVYVLDYVFGWS 579

BLAST of Carg14720 vs. ExPASy TrEMBL
Match: A0A5A7VFE4 (Sodium/calcium exchanger family protein OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold384G001180 PE=4 SV=1)

HSP 1 Score: 488.4 bits (1256), Expect = 2.0e-134
Identity = 254/281 (90.39%), Postives = 272/281 (96.80%), Query Frame = 0

Query: 3   IHDRLFSRIDANKNGLLSASELRALIVGIQFEAIDLDHDDAVNKILSDFDTSRDNHVDST 62
           I ++LFSRID NK+GLLSASELRALIVGIQF+ IDLDHDDAV+KI++DFDTSRD+HVDS 
Sbjct: 310 IIEKLFSRIDVNKDGLLSASELRALIVGIQFDEIDLDHDDAVDKIMNDFDTSRDSHVDSN 369

Query: 63  EFANGIIRWLAQVQGARTGRADDGPHTLKYLHNFHQETKREHDLLDVGEQNDEVVEGVEE 122
           EF NGIIRWL+QVQG+RTGR DDGPHT+KYLHNFHQETKREHDLLDVGEQ+DEVVEGVEE
Sbjct: 370 EFGNGIIRWLSQVQGSRTGRGDDGPHTMKYLHNFHQETKREHDLLDVGEQSDEVVEGVEE 429

Query: 123 SKGVLIRAILFLLLGTAIAAAFADPLVDVVHNFSNATSIPAFFISFIALPLATNSSEAVS 182
            KGVLI+AILFLLLGTAIAAAFADPLVDVVHNFSNAT IPAFFISFIALPLATNSSEAVS
Sbjct: 430 GKGVLIKAILFLLLGTAIAAAFADPLVDVVHNFSNATKIPAFFISFIALPLATNSSEAVS 489

Query: 183 AIIFASRDKRKTASLTFSELYGAVTMNNVLCLSVFLALVYLRGLVWNFSSEVLVILVVTM 242
           AIIFASRDKRKTASLTFSELYGAVTMNNVLCLSVFLALVYLRGLVWNFSSEVLVILVVT+
Sbjct: 490 AIIFASRDKRKTASLTFSELYGAVTMNNVLCLSVFLALVYLRGLVWNFSSEVLVILVVTV 549

Query: 243 IMGLLGSFRTAFPLWTALVALLLYPLSLVLVYVLDYVFGWS 284
           +MG++GSFRTAFPLWT+LVALLLYP SLVLVYVLDYVFGWS
Sbjct: 550 VMGVMGSFRTAFPLWTSLVALLLYPFSLVLVYVLDYVFGWS 590

BLAST of Carg14720 vs. ExPASy TrEMBL
Match: E5GBG6 (Sodium/calcium exchanger family protein OS=Cucumis melo subsp. melo OX=412675 PE=4 SV=1)

HSP 1 Score: 488.4 bits (1256), Expect = 2.0e-134
Identity = 254/281 (90.39%), Postives = 272/281 (96.80%), Query Frame = 0

Query: 3   IHDRLFSRIDANKNGLLSASELRALIVGIQFEAIDLDHDDAVNKILSDFDTSRDNHVDST 62
           I ++LFSRID NK+GLLSASELRALIVGIQF+ IDLDHDDAV+KI++DFDTSRD+HVDS 
Sbjct: 305 IIEKLFSRIDVNKDGLLSASELRALIVGIQFDEIDLDHDDAVDKIMNDFDTSRDSHVDSN 364

Query: 63  EFANGIIRWLAQVQGARTGRADDGPHTLKYLHNFHQETKREHDLLDVGEQNDEVVEGVEE 122
           EF NGIIRWL+QVQG+RTGR DDGPHT+KYLHNFHQETKREHDLLDVGEQ+DEVVEGVEE
Sbjct: 365 EFGNGIIRWLSQVQGSRTGRGDDGPHTMKYLHNFHQETKREHDLLDVGEQSDEVVEGVEE 424

Query: 123 SKGVLIRAILFLLLGTAIAAAFADPLVDVVHNFSNATSIPAFFISFIALPLATNSSEAVS 182
            KGVLI+AILFLLLGTAIAAAFADPLVDVVHNFSNAT IPAFFISFIALPLATNSSEAVS
Sbjct: 425 GKGVLIKAILFLLLGTAIAAAFADPLVDVVHNFSNATKIPAFFISFIALPLATNSSEAVS 484

Query: 183 AIIFASRDKRKTASLTFSELYGAVTMNNVLCLSVFLALVYLRGLVWNFSSEVLVILVVTM 242
           AIIFASRDKRKTASLTFSELYGAVTMNNVLCLSVFLALVYLRGLVWNFSSEVLVILVVT+
Sbjct: 485 AIIFASRDKRKTASLTFSELYGAVTMNNVLCLSVFLALVYLRGLVWNFSSEVLVILVVTV 544

Query: 243 IMGLLGSFRTAFPLWTALVALLLYPLSLVLVYVLDYVFGWS 284
           +MG++GSFRTAFPLWT+LVALLLYP SLVLVYVLDYVFGWS
Sbjct: 545 VMGVMGSFRTAFPLWTSLVALLLYPFSLVLVYVLDYVFGWS 585

BLAST of Carg14720 vs. ExPASy TrEMBL
Match: A0A1S3CJX3 (uncharacterized protein LOC103501799 OS=Cucumis melo OX=3656 GN=LOC103501799 PE=4 SV=1)

HSP 1 Score: 488.4 bits (1256), Expect = 2.0e-134
Identity = 254/281 (90.39%), Postives = 272/281 (96.80%), Query Frame = 0

Query: 3   IHDRLFSRIDANKNGLLSASELRALIVGIQFEAIDLDHDDAVNKILSDFDTSRDNHVDST 62
           I ++LFSRID NK+GLLSASELRALIVGIQF+ IDLDHDDAV+KI++DFDTSRD+HVDS 
Sbjct: 310 IIEKLFSRIDVNKDGLLSASELRALIVGIQFDEIDLDHDDAVDKIMNDFDTSRDSHVDSN 369

Query: 63  EFANGIIRWLAQVQGARTGRADDGPHTLKYLHNFHQETKREHDLLDVGEQNDEVVEGVEE 122
           EF NGIIRWL+QVQG+RTGR DDGPHT+KYLHNFHQETKREHDLLDVGEQ+DEVVEGVEE
Sbjct: 370 EFGNGIIRWLSQVQGSRTGRGDDGPHTMKYLHNFHQETKREHDLLDVGEQSDEVVEGVEE 429

Query: 123 SKGVLIRAILFLLLGTAIAAAFADPLVDVVHNFSNATSIPAFFISFIALPLATNSSEAVS 182
            KGVLI+AILFLLLGTAIAAAFADPLVDVVHNFSNAT IPAFFISFIALPLATNSSEAVS
Sbjct: 430 GKGVLIKAILFLLLGTAIAAAFADPLVDVVHNFSNATKIPAFFISFIALPLATNSSEAVS 489

Query: 183 AIIFASRDKRKTASLTFSELYGAVTMNNVLCLSVFLALVYLRGLVWNFSSEVLVILVVTM 242
           AIIFASRDKRKTASLTFSELYGAVTMNNVLCLSVFLALVYLRGLVWNFSSEVLVILVVT+
Sbjct: 490 AIIFASRDKRKTASLTFSELYGAVTMNNVLCLSVFLALVYLRGLVWNFSSEVLVILVVTV 549

Query: 243 IMGLLGSFRTAFPLWTALVALLLYPLSLVLVYVLDYVFGWS 284
           +MG++GSFRTAFPLWT+LVALLLYP SLVLVYVLDYVFGWS
Sbjct: 550 VMGVMGSFRTAFPLWTSLVALLLYPFSLVLVYVLDYVFGWS 590

BLAST of Carg14720 vs. TAIR 10
Match: AT1G53210.1 (sodium/calcium exchanger family protein / calcium-binding EF hand family protein )

HSP 1 Score: 335.5 bits (859), Expect = 4.2e-92
Identity = 184/280 (65.71%), Postives = 212/280 (75.71%), Query Frame = 0

Query: 6   RLFSRIDANKNGLLSASELRALIVGIQFEAIDLDHDDAVNKILSDFDTSRDNHVDSTEFA 65
           +LF  IDAN +G LSA+EL+ALI+GI FE ID D DDAV K+L DFD + D  VD  EF 
Sbjct: 306 KLFLTIDANNDGHLSAAELKALIIGISFEDIDFDKDDAVGKVLQDFDKTLDEQVDQEEFV 365

Query: 66  NGIIRWLAQVQGARTGRADDGPHTLKYLHNFHQETKREHDLLDVGE--QNDEVVEGVEES 125
            GI +WL Q  G      + GP T+K+L NFH +TKREH LL   E  +NDE    V + 
Sbjct: 366 RGIKQWLIQAMGGAPSGPEAGPRTMKFLDNFHVQTKREHALLGDNENGENDEEGGEVADP 425

Query: 126 KGVLIRAILFLLLGTAIAAAFADPLVDVVHNFSNATSIPAFFISFIALPLATNSSEAVSA 185
           K + I+A L LLLG AIAAAFADPLVD V+NFS AT IP+FFISFIALPLATNSSEAVSA
Sbjct: 426 KWITIKAALLLLLGAAIAAAFADPLVDTVNNFSAATGIPSFFISFIALPLATNSSEAVSA 485

Query: 186 IIFASRDKRKTASLTFSELYGAVTMNNVLCLSVFLALVYLRGLVWNFSSEVLVILVVTMI 245
           IIFASR K +TASLTFSEL G VTMNN+LCLSVFLA+VY+RGL WNFSSEVLVIL+V ++
Sbjct: 486 IIFASRKKIRTASLTFSELCGGVTMNNILCLSVFLAIVYVRGLTWNFSSEVLVILIVCLV 545

Query: 246 MGLLGSFRTAFPLWTALVALLLYPLSLVLVYVLDYVFGWS 284
           MG   SFRT +PLWT  +A LLYP SL LVY+LDY FGWS
Sbjct: 546 MGGFASFRTTYPLWTCFIAYLLYPFSLGLVYILDYWFGWS 585

BLAST of Carg14720 vs. TAIR 10
Match: AT1G29025.1 (Calcium-binding EF-hand family protein )

HSP 1 Score: 144.4 bits (363), Expect = 1.4e-34
Identity = 89/273 (32.60%), Postives = 158/273 (57.88%), Query Frame = 0

Query: 7   LFSRIDANKNGLLSASELRALIVGI-QFEAIDLDHDDAVNKILSDFDTSRDNHVDSTEFA 66
           LF + D NK+G +  SEL+ L V       +  D ++    +L+DFD++RD  +D TEFA
Sbjct: 313 LFKKFDVNKDGKIQVSELKDLTVDFGVLGRVKCDINELATTLLADFDSNRDGEIDETEFA 372

Query: 67  NGIIRWLAQVQGARTGRADDGPHTLKYLHNFHQETKREHDLLDVGEQNDEVVEGVEESKG 126
            GI +WL Q + +  G    G           ++   +  +L V    +++++G   +K 
Sbjct: 373 IGIEKWLKQYKFSFNGTESQG-----------EDIAEDDGVLKV----EQLLKGCLFTKL 432

Query: 127 V---LIRAILFLLLGTAIAAAFADPLVDVVHNFSNATSIPAFFISFIALPLATNSSEAVS 186
           +    ++A++ +++G  I +  A P +  +   S +  +P+F++ F  +PLA N   A+S
Sbjct: 433 LTKRTLKAVIEVIIGITIVSFLAMPFMMNIELLSVSAGVPSFYVVFAVIPLARNLKNALS 492

Query: 187 AIIFASRDKRKTASLTFSELYGAVTMNNVLCLSVFLALVYLRGLVWNFSSEVLVILVVTM 246
           A     ++K K  S TFSE+Y  VT+NN+L +++ LA+VY+RGL W++S+EVL+I++V +
Sbjct: 493 AHFCRKKEKAKITSDTFSEIYRDVTLNNLLGITIILAIVYIRGLTWDYSTEVLIIVIVGL 552

Query: 247 IMGLLGSFRTAFPLWTALVALLLYPLSLVLVYV 276
           I+G+    R+ +P W  ++A  LY  SL+L+Y+
Sbjct: 553 IIGVPAYVRSTYPFWICVLAFALYFFSLILIYL 570

BLAST of Carg14720 vs. TAIR 10
Match: AT1G29020.1 (Calcium-binding EF-hand family protein )

HSP 1 Score: 134.4 bits (337), Expect = 1.4e-31
Identity = 92/276 (33.33%), Postives = 145/276 (52.54%), Query Frame = 0

Query: 6   RLFSRIDANKNGLLSASELRALIVGI-QFEAIDLDHDDAVNKILSDFDTSRDNHVDSTEF 65
           RLF +IDAN +G +  +EL+ L V       +    D  V+ ++ DFD   D  +D  EF
Sbjct: 292 RLFQKIDANGDGKIQVAELKDLTVEFGMIGRVKCHIDKLVHTLVDDFDRDGDREIDEAEF 351

Query: 66  ANGIIRWLAQVQGARTGRADDGPHTLKYLHNFHQETKREHD------LLDVGEQNDEVVE 125
             GI +WL Q                 Y  +F        D      +L V +    +V+
Sbjct: 352 EIGIKKWLNQ-----------------YKFSFDTTVPPREDQAEGVPILKVEKPEQSLVK 411

Query: 126 GVEESKGVLIRAILFLLLGTAIAAAFADPLVDVVHNFSNATSIPAFFISFIALPLATNSS 185
            +   +   +RA L ++LG  +    A P +  +   S +  +P+F++ F+ +PLA N  
Sbjct: 412 KLLSWR--TLRASLEVILGMLVVIYLARPFMMNIQLLSESAGVPSFYVVFVVIPLARNLK 471

Query: 186 EAVSAIIFASRDKRKTASLTFSELYGAVTMNNVLCLSVFLALVYLRGLVWNFSSEVLVIL 245
             +SA     +DK K  S TFSE+Y  VTMNN++ +S+ LA+VY R LVW++++EVL+I+
Sbjct: 472 NTLSAHFCRKKDKAKITSDTFSEIYKDVTMNNLMGISIILAIVYARELVWDYTTEVLIIV 531

Query: 246 VVTMIMGLLGSFRTAFPLWTALVALLLYPLSLVLVY 275
           +V +++GL    RT +P W  L+A  LY +SLV+VY
Sbjct: 532 IVGLLIGLPAYVRTTYPFWICLLAFALYFISLVVVY 548

BLAST of Carg14720 vs. TAIR 10
Match: AT2G34030.1 (Calcium-binding EF-hand family protein )

HSP 1 Score: 120.9 bits (302), Expect = 1.6e-27
Identity = 86/279 (30.82%), Postives = 140/279 (50.18%), Query Frame = 0

Query: 7   LFSRIDANKNGLLSASELRALIVGI-QFEAIDLDHDDAVNKILSDFDTSRDNHVDSTEFA 66
           LF + D NK+G +  SEL+ L + +  F  +  D ++     L DFD   D  ++  EF 
Sbjct: 304 LFKKTDKNKDGKIQISELKDLTIELSNFGRMRYDINELAKAFLEDFDGDNDGELEENEFE 363

Query: 67  NGIIRWLAQVQGARTGRADDGPHTLKYLHNFHQETKREHDLLDVGEQNDEVVEGVEESKG 126
            GI R L Q                   + F+ E +RE+   + G    E+     + K 
Sbjct: 364 EGIARLLKQ-------------------YKFNVEDQRENQTEENGVLKLEI-----KPKK 423

Query: 127 VLIRAILFL---------LLGTAIAAAFADPLVDVVHNFSNATSIPAFFISFIALPLATN 186
            L+  +L +         ++G  I    A P +  +   S +  IP+F+I F  +P A N
Sbjct: 424 TLVTKLLSMETLIATTEVIVGILIVLFLAKPFMLNIQLLSISAGIPSFYIVFAMIPFARN 483

Query: 187 SSEAVSAIIFASRDKRKTASLTFSELYGAVTMNNVLCLSVFLALVYLRGLVWNFSSEVLV 246
               +S      +DK++ +S TFSE+Y  +TMNN+L +SV LA+VY RGL W +S E L+
Sbjct: 484 LKNTLSTRFCRGKDKKRVSSNTFSEIYKDITMNNLLGMSVILAIVYTRGLTWKYSVETLI 543

Query: 247 ILVVTMIMGLLGSFRTAFPLWTALVALLLYPLSLVLVYV 276
           +++V +I+GL    R+ +P W  ++A  +Y  SL+L+Y+
Sbjct: 544 MVIVGLIIGLPIYIRSTYPFWMCVLAFAMYFFSLLLIYI 558

BLAST of Carg14720 vs. TAIR 10
Match: AT2G34020.1 (Calcium-binding EF-hand family protein )

HSP 1 Score: 113.6 bits (283), Expect = 2.6e-25
Identity = 87/287 (30.31%), Postives = 147/287 (51.22%), Query Frame = 0

Query: 7   LFSRIDANKNGLLSASELRALIVGI-QFEAIDLDHDDAVNKILSDFDTSRDNHVDSTEFA 66
           LF +ID NK+G +  SEL+ L V    F  +  D ++  + +L++FD  ++  +D  EF 
Sbjct: 332 LFDKIDRNKDGKIQISELKDLTVEFGVFGKMKCDINEFASTLLAEFDKDKNGELDENEFE 391

Query: 67  NGIIRWLAQVQGARTGRADDGPHTLKYLHNFHQETKREHDLLDVGEQNDEVVEGVEESKG 126
            GI++ L      +    +       Y++    ++ +    L  GE+    V  +E  K 
Sbjct: 392 EGIMKLLNHY---KFDNQESPRQNNTYIYRTPSDSVKS---LSQGEEAG--VLKLEMPKQ 451

Query: 127 VLI---------RAILFLLLGTAIAAAFADPLVDVVHNFSNATSIPAFFISFIALPLATN 186
            L+         RA+  ++ G  I    A P +  +   S +  +P+F+  F  +PL  N
Sbjct: 452 TLVAKFLSMSTLRAVTKVIGGMLIVVFLAKPFMVNIGLLSVSAGVPSFYSVFAVIPLVRN 511

Query: 187 SSEAVSAIIFASRDKRKTASLTFSELYGAVTMNNVLCLSVFLALVYLRGLVWNFSSEVLV 246
               +SA     +DK + AS  FSE+Y  VTMNN++ +S+ LA+VY RGL W +S E L+
Sbjct: 512 LKNTLSAHFCRKKDKARIASEKFSEIYRDVTMNNLMGMSITLAIVYSRGLKWEYSIESLL 571

Query: 247 ILVVTMIMGLLGSFRTAFPLWTALVALLLYPLSLVLVYVLDYVFGWS 284
           ++VV + +GL    R+ +P W  ++A  +Y  SLVL+Y+  ++ G S
Sbjct: 572 VVVVGIAIGLPAYVRSTYPFWICVMAFAMYIFSLVLIYIHFHLRGQS 610

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
KAG7037430.11.9e-147100.00Sodium/calcium exchanger NCL1, partial [Cucurbita argyrosperma subsp. argyrosper... [more]
KAG6607902.14.4e-14498.93Sodium/calcium exchanger NCL, partial [Cucurbita argyrosperma subsp. sororia][more]
XP_022941280.11.7e-14398.58sodium/calcium exchanger NCL-like [Cucurbita moschata][more]
XP_022981259.11.8e-14298.92sodium/calcium exchanger NCL-like [Cucurbita maxima][more]
XP_023523899.13.1e-14297.51sodium/calcium exchanger NCL-like [Cucurbita pepo subsp. pepo][more]
Match NameE-valueIdentityDescription
Q8L6365.8e-9165.71Sodium/calcium exchanger NCL OS=Arabidopsis thaliana OX=3702 GN=NCL PE=2 SV=1[more]
Q5QNI26.3e-8561.15Sodium/calcium exchanger NCL1 OS=Oryza sativa subsp. japonica OX=39947 GN=NCL1 P... [more]
Q6K3R57.2e-8159.15Sodium/calcium exchanger NCL2 OS=Oryza sativa subsp. japonica OX=39947 GN=NCL2 P... [more]
Q5KQN04.2e-0429.50Vacuolar cation/proton exchanger 2 OS=Oryza sativa subsp. japonica OX=39947 GN=C... [more]
Match NameE-valueIdentityDescription
A0A6J1FT508.1e-14498.58sodium/calcium exchanger NCL-like OS=Cucurbita moschata OX=3662 GN=LOC111446626 ... [more]
A0A6J1J1E58.9e-14398.92sodium/calcium exchanger NCL-like OS=Cucurbita maxima OX=3661 GN=LOC111480448 PE... [more]
A0A5A7VFE42.0e-13490.39Sodium/calcium exchanger family protein OS=Cucumis melo var. makuwa OX=1194695 G... [more]
E5GBG62.0e-13490.39Sodium/calcium exchanger family protein OS=Cucumis melo subsp. melo OX=412675 PE... [more]
A0A1S3CJX32.0e-13490.39uncharacterized protein LOC103501799 OS=Cucumis melo OX=3656 GN=LOC103501799 PE=... [more]
Match NameE-valueIdentityDescription
AT1G53210.14.2e-9265.71sodium/calcium exchanger family protein / calcium-binding EF hand family protein... [more]
AT1G29025.11.4e-3432.60Calcium-binding EF-hand family protein [more]
AT1G29020.11.4e-3133.33Calcium-binding EF-hand family protein [more]
AT2G34030.11.6e-2730.82Calcium-binding EF-hand family protein [more]
AT2G34020.12.6e-2530.31Calcium-binding EF-hand family protein [more]
InterPro
Analysis Name: InterPro Annotations of Silver-seed gourd (SMH-JMG-627) v2
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR002048EF-hand domainPFAMPF13202EF-hand_5coord: 5..27
e-value: 2.5E-4
score: 20.4
IPR002048EF-hand domainPROSITEPS50222EF_HAND_2coord: 1..34
score: 9.826979
IPR002048EF-hand domainPROSITEPS50222EF_HAND_2coord: 39..74
score: 8.209045
IPR004837Sodium/calcium exchanger membrane regionPFAMPF01699Na_Ca_excoord: 131..274
e-value: 1.2E-11
score: 44.8
NoneNo IPR availableGENE3D1.10.238.10coord: 2..108
e-value: 3.1E-9
score: 38.6
NoneNo IPR availablePANTHERPTHR31503:SF36SODIUM/CALCIUM EXCHANGER NCLcoord: 11..280
IPR004713Calcium/proton exchangerPANTHERPTHR31503VACUOLAR CALCIUM ION TRANSPORTERcoord: 11..280
IPR018247EF-Hand 1, calcium-binding sitePROSITEPS00018EF_HAND_1coord: 12..24
IPR018247EF-Hand 1, calcium-binding sitePROSITEPS00018EF_HAND_1coord: 52..64
IPR011992EF-hand domain pairSUPERFAMILY47473EF-handcoord: 6..71

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Carg14720-RACarg14720-RAmRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0070588 calcium ion transmembrane transport
biological_process GO:0006874 cellular calcium ion homeostasis
biological_process GO:0006812 cation transport
biological_process GO:0055085 transmembrane transport
cellular_component GO:0016021 integral component of membrane
cellular_component GO:0005774 vacuolar membrane
molecular_function GO:0005509 calcium ion binding
molecular_function GO:0015369 calcium:proton antiporter activity
molecular_function GO:0008324 cation transmembrane transporter activity