Carg14643 (gene) Silver-seed gourd (SMH-JMG-627) v2

Overview
NameCarg14643
Typegene
OrganismCucurbita argyrosperma subsp. argyrosperma cv. SMH-JMG-627 (Silver-seed gourd (SMH-JMG-627) v2)
Descriptionpolyphenol oxidase, chloroplastic-like
LocationCarg_Chr18: 2058092 .. 2059801 (-)
RNA-Seq ExpressionCarg14643
SyntenyCarg14643
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGCCACTCAAAACAATGGCTTCTATATCTCCTCTTCTATCCATTCTCCCATACTCCCAAATCAAAAACCAAACATCCAACCTTCAAAACAAAAGCCGTGGCATCAACCATGTTCCATGCAGAGCCTCCAATGGAGACTCCACAAGCAAGTTTGATAGAAGAGATGTGCTGTTTGGACTGGGCGGACTTTATGGTGCAGCTGCTCTAACCAACCAACAAAGCCCTGCGTCCGCAGCACCTTTAGCACCCGACGTCAACAACTGCGAGCCAGCCACAATAGAAGGCAACCAACACATCCCATGCTGCCCTCCCAAAATCCATGATATCATTGATTTCATCCATCCACCACACACAACCAAACTCCGCATTCGCCCACCCGCCCACTCGCTTACTCCAAAGCAACAATTCAACTTCGAGAAAGCCATTCATATCATGAAACGCCTCCCTCCCAACGACCCTCGTAACTTTTACAGGCAAGCCGATGTTCACTGTGCCTATTGTGATTATGCCTACAAACAATTAGGCTTCCCAAACGCCGAAATGCAAGTCCATTGGAACGCTTTGTTCTTCCCATTTCACCGAGCTTACCTCTACTTCTTCGAGAGAATCTTAGGCCATTTAATTAACGAACCAAATTTCGTCATACCCTTTTGGAATTGGGATAATACTGAAGGTATGCAAATGCCAGAAATCTACGACCACCCAAATTCGCCACTCTATGACAAGCTACGTAATCAAAGGCATAGGCCACATAAATTGCTTAACCTTAACTACCACGGCGACGACAACCCTTCATACGACGTCGTCGATTCTAATCTCCGGTGGATGCATAAGCAAATGATAACGGACGCCAATACTGCTGAAGGCTTCCATGGAAAGCTACTCATTGCATCTCATGAAGCTAATGAAGAAACTGGGATGGGTACCATTGAGAGATCTCCTCATAATAATATTCACAATTGGGTTGGCGATCCAAACGCCAAGAACCATATGAACATGGGAGCGTTTTACTCGGCAGCTCGAGATCCGTTGTTTTATGGTCATCATGCGAATGTGGATCGCATGTGGAGCATTTGGCAAGGGTTAAAGAATGGGAAGCCAAGAGATTTTGATAACCCCGATTGGTTGAATTCCTCGTTTGTGTTCTATAATGAGCATAAACAAGCTGTACGCATCAAAGTTAGTGATTGTTTGGACACTAGAAGGCTTGGATATGTTTACCAATATGTAGATCTTCCATGGCTGCATACTAAGCAAACCCCACGTCTAAAAAAGCAGCTGAAAACAACGCAAGCTCCACAAGTTATTAAATTTCCTCAGGGTCTCAATTCAATGGTAACCACAACGGTGAAGAGACCGAACAAATTAATGAATGAAAAGGAAAAGAGTGAGAAAGATGAGGTATTGGTGATAGAAGGCATTGAATTGGATAAGAGATTTTGGGTTCATTTTAATGTGCTTGTTAATGTTGTTGATGATGGCTCCGATGTGTGTCCGGGGAATACTGAGTTTGTAGGAACGTTTACGACCGTCCCGCATGGTCACAACCACCGTGGAAAGACGTCTTTACGAGTTGTGATAACGGAGGTGCTTAAAGAGTTGGGAATAGAGGACGATGAGAGTGTGATTGTGAGGCTCGACCCTAAGGTTGGTGGAGACAAGGTCACCATTGGAGGGATGAAGATCGAACATCAGCCAAGAGGTCAGTAG

mRNA sequence

ATGCCACTCAAAACAATGGCTTCTATATCTCCTCTTCTATCCATTCTCCCATACTCCCAAATCAAAAACCAAACATCCAACCTTCAAAACAAAAGCCGTGGCATCAACCATGTTCCATGCAGAGCCTCCAATGGAGACTCCACAAGCAAGTTTGATAGAAGAGATGTGCTGTTTGGACTGGGCGGACTTTATGGTGCAGCTGCTCTAACCAACCAACAAAGCCCTGCGTCCGCAGCACCTTTAGCACCCGACGTCAACAACTGCGAGCCAGCCACAATAGAAGGCAACCAACACATCCCATGCTGCCCTCCCAAAATCCATGATATCATTGATTTCATCCATCCACCACACACAACCAAACTCCGCATTCGCCCACCCGCCCACTCGCTTACTCCAAAGCAACAATTCAACTTCGAGAAAGCCATTCATATCATGAAACGCCTCCCTCCCAACGACCCTCGTAACTTTTACAGGCAAGCCGATGTTCACTGTGCCTATTGTGATTATGCCTACAAACAATTAGGCTTCCCAAACGCCGAAATGCAAGTCCATTGGAACGCTTTGTTCTTCCCATTTCACCGAGCTTACCTCTACTTCTTCGAGAGAATCTTAGGCCATTTAATTAACGAACCAAATTTCGTCATACCCTTTTGGAATTGGGATAATACTGAAGGTATGCAAATGCCAGAAATCTACGACCACCCAAATTCGCCACTCTATGACAAGCTACGTAATCAAAGGCATAGGCCACATAAATTGCTTAACCTTAACTACCACGGCGACGACAACCCTTCATACGACGTCGTCGATTCTAATCTCCGGTGGATGCATAAGCAAATGATAACGGACGCCAATACTGCTGAAGGCTTCCATGGAAAGCTACTCATTGCATCTCATGAAGCTAATGAAGAAACTGGGATGGGTACCATTGAGAGATCTCCTCATAATAATATTCACAATTGGGTTGGCGATCCAAACGCCAAGAACCATATGAACATGGGAGCGTTTTACTCGGCAGCTCGAGATCCGTTGTTTTATGGTCATCATGCGAATGTGGATCGCATGTGGAGCATTTGGCAAGGGTTAAAGAATGGGAAGCCAAGAGATTTTGATAACCCCGATTGGTTGAATTCCTCGTTTGTGTTCTATAATGAGCATAAACAAGCTGTACGCATCAAAGTTAGTGATTGTTTGGACACTAGAAGGCTTGGATATGTTTACCAATATGTAGATCTTCCATGGCTGCATACTAAGCAAACCCCACGTCTAAAAAAGCAGCTGAAAACAACGCAAGCTCCACAAGTTATTAAATTTCCTCAGGGTCTCAATTCAATGGTAACCACAACGGTGAAGAGACCGAACAAATTAATGAATGAAAAGGAAAAGAGTGAGAAAGATGAGGTATTGGTGATAGAAGGCATTGAATTGGATAAGAGATTTTGGGTTCATTTTAATGTGCTTGTTAATGTTGTTGATGATGGCTCCGATGTGTGTCCGGGGAATACTGAGTTTGTAGGAACGTTTACGACCGTCCCGCATGGTCACAACCACCGTGGAAAGACGTCTTTACGAGTTGTGATAACGGAGGTGCTTAAAGAGTTGGGAATAGAGGACGATGAGAGTGTGATTGTGAGGCTCGACCCTAAGGTTGGTGGAGACAAGGTCACCATTGGAGGGATGAAGATCGAACATCAGCCAAGAGGTCAGTAG

Coding sequence (CDS)

ATGCCACTCAAAACAATGGCTTCTATATCTCCTCTTCTATCCATTCTCCCATACTCCCAAATCAAAAACCAAACATCCAACCTTCAAAACAAAAGCCGTGGCATCAACCATGTTCCATGCAGAGCCTCCAATGGAGACTCCACAAGCAAGTTTGATAGAAGAGATGTGCTGTTTGGACTGGGCGGACTTTATGGTGCAGCTGCTCTAACCAACCAACAAAGCCCTGCGTCCGCAGCACCTTTAGCACCCGACGTCAACAACTGCGAGCCAGCCACAATAGAAGGCAACCAACACATCCCATGCTGCCCTCCCAAAATCCATGATATCATTGATTTCATCCATCCACCACACACAACCAAACTCCGCATTCGCCCACCCGCCCACTCGCTTACTCCAAAGCAACAATTCAACTTCGAGAAAGCCATTCATATCATGAAACGCCTCCCTCCCAACGACCCTCGTAACTTTTACAGGCAAGCCGATGTTCACTGTGCCTATTGTGATTATGCCTACAAACAATTAGGCTTCCCAAACGCCGAAATGCAAGTCCATTGGAACGCTTTGTTCTTCCCATTTCACCGAGCTTACCTCTACTTCTTCGAGAGAATCTTAGGCCATTTAATTAACGAACCAAATTTCGTCATACCCTTTTGGAATTGGGATAATACTGAAGGTATGCAAATGCCAGAAATCTACGACCACCCAAATTCGCCACTCTATGACAAGCTACGTAATCAAAGGCATAGGCCACATAAATTGCTTAACCTTAACTACCACGGCGACGACAACCCTTCATACGACGTCGTCGATTCTAATCTCCGGTGGATGCATAAGCAAATGATAACGGACGCCAATACTGCTGAAGGCTTCCATGGAAAGCTACTCATTGCATCTCATGAAGCTAATGAAGAAACTGGGATGGGTACCATTGAGAGATCTCCTCATAATAATATTCACAATTGGGTTGGCGATCCAAACGCCAAGAACCATATGAACATGGGAGCGTTTTACTCGGCAGCTCGAGATCCGTTGTTTTATGGTCATCATGCGAATGTGGATCGCATGTGGAGCATTTGGCAAGGGTTAAAGAATGGGAAGCCAAGAGATTTTGATAACCCCGATTGGTTGAATTCCTCGTTTGTGTTCTATAATGAGCATAAACAAGCTGTACGCATCAAAGTTAGTGATTGTTTGGACACTAGAAGGCTTGGATATGTTTACCAATATGTAGATCTTCCATGGCTGCATACTAAGCAAACCCCACGTCTAAAAAAGCAGCTGAAAACAACGCAAGCTCCACAAGTTATTAAATTTCCTCAGGGTCTCAATTCAATGGTAACCACAACGGTGAAGAGACCGAACAAATTAATGAATGAAAAGGAAAAGAGTGAGAAAGATGAGGTATTGGTGATAGAAGGCATTGAATTGGATAAGAGATTTTGGGTTCATTTTAATGTGCTTGTTAATGTTGTTGATGATGGCTCCGATGTGTGTCCGGGGAATACTGAGTTTGTAGGAACGTTTACGACCGTCCCGCATGGTCACAACCACCGTGGAAAGACGTCTTTACGAGTTGTGATAACGGAGGTGCTTAAAGAGTTGGGAATAGAGGACGATGAGAGTGTGATTGTGAGGCTCGACCCTAAGGTTGGTGGAGACAAGGTCACCATTGGAGGGATGAAGATCGAACATCAGCCAAGAGGTCAGTAG

Protein sequence

MPLKTMASISPLLSILPYSQIKNQTSNLQNKSRGINHVPCRASNGDSTSKFDRRDVLFGLGGLYGAAALTNQQSPASAAPLAPDVNNCEPATIEGNQHIPCCPPKIHDIIDFIHPPHTTKLRIRPPAHSLTPKQQFNFEKAIHIMKRLPPNDPRNFYRQADVHCAYCDYAYKQLGFPNAEMQVHWNALFFPFHRAYLYFFERILGHLINEPNFVIPFWNWDNTEGMQMPEIYDHPNSPLYDKLRNQRHRPHKLLNLNYHGDDNPSYDVVDSNLRWMHKQMITDANTAEGFHGKLLIASHEANEETGMGTIERSPHNNIHNWVGDPNAKNHMNMGAFYSAARDPLFYGHHANVDRMWSIWQGLKNGKPRDFDNPDWLNSSFVFYNEHKQAVRIKVSDCLDTRRLGYVYQYVDLPWLHTKQTPRLKKQLKTTQAPQVIKFPQGLNSMVTTTVKRPNKLMNEKEKSEKDEVLVIEGIELDKRFWVHFNVLVNVVDDGSDVCPGNTEFVGTFTTVPHGHNHRGKTSLRVVITEVLKELGIEDDESVIVRLDPKVGGDKVTIGGMKIEHQPRGQ
Homology
BLAST of Carg14643 vs. NCBI nr
Match: KAG7012418.1 (hypothetical protein SDJN02_25170, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 1198.3 bits (3099), Expect = 0.0e+00
Identity = 569/569 (100.00%), Postives = 569/569 (100.00%), Query Frame = 0

Query: 1   MPLKTMASISPLLSILPYSQIKNQTSNLQNKSRGINHVPCRASNGDSTSKFDRRDVLFGL 60
           MPLKTMASISPLLSILPYSQIKNQTSNLQNKSRGINHVPCRASNGDSTSKFDRRDVLFGL
Sbjct: 1   MPLKTMASISPLLSILPYSQIKNQTSNLQNKSRGINHVPCRASNGDSTSKFDRRDVLFGL 60

Query: 61  GGLYGAAALTNQQSPASAAPLAPDVNNCEPATIEGNQHIPCCPPKIHDIIDFIHPPHTTK 120
           GGLYGAAALTNQQSPASAAPLAPDVNNCEPATIEGNQHIPCCPPKIHDIIDFIHPPHTTK
Sbjct: 61  GGLYGAAALTNQQSPASAAPLAPDVNNCEPATIEGNQHIPCCPPKIHDIIDFIHPPHTTK 120

Query: 121 LRIRPPAHSLTPKQQFNFEKAIHIMKRLPPNDPRNFYRQADVHCAYCDYAYKQLGFPNAE 180
           LRIRPPAHSLTPKQQFNFEKAIHIMKRLPPNDPRNFYRQADVHCAYCDYAYKQLGFPNAE
Sbjct: 121 LRIRPPAHSLTPKQQFNFEKAIHIMKRLPPNDPRNFYRQADVHCAYCDYAYKQLGFPNAE 180

Query: 181 MQVHWNALFFPFHRAYLYFFERILGHLINEPNFVIPFWNWDNTEGMQMPEIYDHPNSPLY 240
           MQVHWNALFFPFHRAYLYFFERILGHLINEPNFVIPFWNWDNTEGMQMPEIYDHPNSPLY
Sbjct: 181 MQVHWNALFFPFHRAYLYFFERILGHLINEPNFVIPFWNWDNTEGMQMPEIYDHPNSPLY 240

Query: 241 DKLRNQRHRPHKLLNLNYHGDDNPSYDVVDSNLRWMHKQMITDANTAEGFHGKLLIASHE 300
           DKLRNQRHRPHKLLNLNYHGDDNPSYDVVDSNLRWMHKQMITDANTAEGFHGKLLIASHE
Sbjct: 241 DKLRNQRHRPHKLLNLNYHGDDNPSYDVVDSNLRWMHKQMITDANTAEGFHGKLLIASHE 300

Query: 301 ANEETGMGTIERSPHNNIHNWVGDPNAKNHMNMGAFYSAARDPLFYGHHANVDRMWSIWQ 360
           ANEETGMGTIERSPHNNIHNWVGDPNAKNHMNMGAFYSAARDPLFYGHHANVDRMWSIWQ
Sbjct: 301 ANEETGMGTIERSPHNNIHNWVGDPNAKNHMNMGAFYSAARDPLFYGHHANVDRMWSIWQ 360

Query: 361 GLKNGKPRDFDNPDWLNSSFVFYNEHKQAVRIKVSDCLDTRRLGYVYQYVDLPWLHTKQT 420
           GLKNGKPRDFDNPDWLNSSFVFYNEHKQAVRIKVSDCLDTRRLGYVYQYVDLPWLHTKQT
Sbjct: 361 GLKNGKPRDFDNPDWLNSSFVFYNEHKQAVRIKVSDCLDTRRLGYVYQYVDLPWLHTKQT 420

Query: 421 PRLKKQLKTTQAPQVIKFPQGLNSMVTTTVKRPNKLMNEKEKSEKDEVLVIEGIELDKRF 480
           PRLKKQLKTTQAPQVIKFPQGLNSMVTTTVKRPNKLMNEKEKSEKDEVLVIEGIELDKRF
Sbjct: 421 PRLKKQLKTTQAPQVIKFPQGLNSMVTTTVKRPNKLMNEKEKSEKDEVLVIEGIELDKRF 480

Query: 481 WVHFNVLVNVVDDGSDVCPGNTEFVGTFTTVPHGHNHRGKTSLRVVITEVLKELGIEDDE 540
           WVHFNVLVNVVDDGSDVCPGNTEFVGTFTTVPHGHNHRGKTSLRVVITEVLKELGIEDDE
Sbjct: 481 WVHFNVLVNVVDDGSDVCPGNTEFVGTFTTVPHGHNHRGKTSLRVVITEVLKELGIEDDE 540

Query: 541 SVIVRLDPKVGGDKVTIGGMKIEHQPRGQ 570
           SVIVRLDPKVGGDKVTIGGMKIEHQPRGQ
Sbjct: 541 SVIVRLDPKVGGDKVTIGGMKIEHQPRGQ 569

BLAST of Carg14643 vs. NCBI nr
Match: XP_022954613.1 (polyphenol oxidase, chloroplastic-like [Cucurbita moschata])

HSP 1 Score: 1170.2 bits (3026), Expect = 0.0e+00
Identity = 556/564 (98.58%), Postives = 559/564 (99.11%), Query Frame = 0

Query: 6   MASISPLLSILPYSQIKNQTSNLQNKSRGINHVPCRASNGDSTSKFDRRDVLFGLGGLYG 65
           MASISPLLSILPYSQIKNQTSNLQNKSRGIN VPCRASNGDSTSKFDRRDVLFGLGGLYG
Sbjct: 1   MASISPLLSILPYSQIKNQTSNLQNKSRGINQVPCRASNGDSTSKFDRRDVLFGLGGLYG 60

Query: 66  AAALTNQQSPASAAPLAPDVNNCEPATIEGNQHIPCCPPKIHDIIDFIHPPHTTKLRIRP 125
           AAALTNQQSPASAAPLAPDVNNCE ATIEGN+HIPCCPPKIHDIIDFIHPPHTTKLRIRP
Sbjct: 61  AAALTNQQSPASAAPLAPDVNNCEAATIEGNEHIPCCPPKIHDIIDFIHPPHTTKLRIRP 120

Query: 126 PAHSLTPKQQFNFEKAIHIMKRLPPNDPRNFYRQADVHCAYCDYAYKQLGFPNAEMQVHW 185
           PAHSLTPKQQFNFEKAIHIMKRLPPNDPRNFYRQADVHCAYCDYAYKQLGFPNAEMQVHW
Sbjct: 121 PAHSLTPKQQFNFEKAIHIMKRLPPNDPRNFYRQADVHCAYCDYAYKQLGFPNAEMQVHW 180

Query: 186 NALFFPFHRAYLYFFERILGHLINEPNFVIPFWNWDNTEGMQMPEIYDHPNSPLYDKLRN 245
           NALFFPFHRAYLYFFERILGHLINEPNFVIPFWNWDNTEGMQMPEIYDHPNSPLYDKLRN
Sbjct: 181 NALFFPFHRAYLYFFERILGHLINEPNFVIPFWNWDNTEGMQMPEIYDHPNSPLYDKLRN 240

Query: 246 QRHRPHKLLNLNYHGDDNPSYDVVDSNLRWMHKQMITDANTAEGFHGKLLIASHEANEET 305
           QRHRPHKLLNLNYHGDDNPSYDVVDSNLRWMHKQMITDANTAEGFHGKLLIASHEANEET
Sbjct: 241 QRHRPHKLLNLNYHGDDNPSYDVVDSNLRWMHKQMITDANTAEGFHGKLLIASHEANEET 300

Query: 306 GMGTIERSPHNNIHNWVGDPNAKNHMNMGAFYSAARDPLFYGHHANVDRMWSIWQGLKNG 365
           GMGTIE+SPHNNIHNWVGDPNAKNHMNMGAFYSAARDPLFYGHHANVDRMWSIWQGLKNG
Sbjct: 301 GMGTIEKSPHNNIHNWVGDPNAKNHMNMGAFYSAARDPLFYGHHANVDRMWSIWQGLKNG 360

Query: 366 KPRDFDNPDWLNSSFVFYNEHKQAVRIKVSDCLDTRRLGYVYQYVDLPWLHTKQTPRLKK 425
           K RDFDNPDWLNSSFVFYNEHKQAVRIKV DCLDTRRLGYVYQYVDLPWLHTKQTPRLKK
Sbjct: 361 KARDFDNPDWLNSSFVFYNEHKQAVRIKVRDCLDTRRLGYVYQYVDLPWLHTKQTPRLKK 420

Query: 426 QLKTTQAPQVIKFPQGLNSMVTTTVKRPNKLMNEKEKSEKDEVLVIEGIELDKRFWVHFN 485
           QLKTTQAPQVIKFPQGLNSMVTTTVKRPNKLMNEKEKSEKDEVLVIEGIELDKRFWVHFN
Sbjct: 421 QLKTTQAPQVIKFPQGLNSMVTTTVKRPNKLMNEKEKSEKDEVLVIEGIELDKRFWVHFN 480

Query: 486 VLVNVVDDGSDVCPGNTEFVGTFTTVPHGHNHRGKTSLRVVITEVLKELGIEDDESVIVR 545
           VLVNVVDDGSDVCPGNTEFVGTFTTVPHGHNHRGKTSLRVVITEVLKELGIEDDESVIVR
Sbjct: 481 VLVNVVDDGSDVCPGNTEFVGTFTTVPHGHNHRGKTSLRVVITEVLKELGIEDDESVIVR 540

Query: 546 LDPKVGGDKVTIGGMKIEHQPRGQ 570
           LDPKVGGDKVTIGGMKIE QP+GQ
Sbjct: 541 LDPKVGGDKVTIGGMKIELQPKGQ 564

BLAST of Carg14643 vs. NCBI nr
Match: KAG6573251.1 (hypothetical protein SDJN03_27138, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 1164.8 bits (3012), Expect = 0.0e+00
Identity = 554/563 (98.40%), Postives = 557/563 (98.93%), Query Frame = 0

Query: 6   MASISPLLSILPYSQIKNQTSNLQNKSRGINHVPCRASNGDSTSKFDRRDVLFGLGGLYG 65
           MASISPLLSILPYSQIKNQTSNLQNKSRGINHVPCRASNGDSTSKFDRRDVLFGLGGLYG
Sbjct: 1   MASISPLLSILPYSQIKNQTSNLQNKSRGINHVPCRASNGDSTSKFDRRDVLFGLGGLYG 60

Query: 66  AAALTNQQSPASAAPLAPDVNNCEPATIEGNQHIPCCPPKIHDIIDFIHPPHTTKLRIRP 125
           AAALTNQQSPASAAPLAPDVNNCEPATIEGNQHIPCCPPKIH IIDFIHPPHTTKLRIRP
Sbjct: 61  AAALTNQQSPASAAPLAPDVNNCEPATIEGNQHIPCCPPKIHHIIDFIHPPHTTKLRIRP 120

Query: 126 PAHSLTPKQQFNFEKAIHIMKRLPPNDPRNFYRQADVHCAYCDYAYKQLGFPNAEMQVHW 185
           PAHSLTPKQQFNFEKAIHIMKRLPPNDPRNFYRQADVHCAYCDYAYKQLGFPNAEMQVHW
Sbjct: 121 PAHSLTPKQQFNFEKAIHIMKRLPPNDPRNFYRQADVHCAYCDYAYKQLGFPNAEMQVHW 180

Query: 186 NALFFPFHRAYLYFFERILGHLINEPNFVIPFWNWDNTEGMQMPEIYDHPNSPLYDKLRN 245
           NALFFPFHRAYLYFFERILGHLINEPNFVIPFWNWDNTEGMQMPEIYDHPNSPLYDKLRN
Sbjct: 181 NALFFPFHRAYLYFFERILGHLINEPNFVIPFWNWDNTEGMQMPEIYDHPNSPLYDKLRN 240

Query: 246 QRHRPHKLLNLNYHGDDNPSYDVVDSNLRWMHKQMITDANTAEGFHGKLLIASHEANEET 305
           QRHRPHKLLNLNYHGDDNPSYDVVDSNLRWMHKQMI DA+TAEGF GKLL+AS EANEET
Sbjct: 241 QRHRPHKLLNLNYHGDDNPSYDVVDSNLRWMHKQMIKDADTAEGFLGKLLVASDEANEET 300

Query: 306 GMGTIERSPHNNIHNWVGDPNAKNHMNMGAFYSAARDPLFYGHHANVDRMWSIWQGLKNG 365
           GMGTIERSPHNNIHNWVGDPNAKNHMNMGAFYSAARDPLFYGHHANVDRMWSIWQGLKNG
Sbjct: 301 GMGTIERSPHNNIHNWVGDPNAKNHMNMGAFYSAARDPLFYGHHANVDRMWSIWQGLKNG 360

Query: 366 KPRDFDNPDWLNSSFVFYNEHKQAVRIKVSDCLDTRRLGYVYQYVDLPWLHTKQTPRLKK 425
           KPRDFDNPDWLNSSFVFYNEHKQAVRIKV DCLDTRRLGYVYQYVDLPWLHTKQTPRLKK
Sbjct: 361 KPRDFDNPDWLNSSFVFYNEHKQAVRIKVRDCLDTRRLGYVYQYVDLPWLHTKQTPRLKK 420

Query: 426 QLKTTQAPQVIKFPQGLNSMVTTTVKRPNKLMNEKEKSEKDEVLVIEGIELDKRFWVHFN 485
           QLKTTQAPQVIKFPQGLNSMVTTTVKRPNKLMNEKEKSEKDEVLVIEGIELDKRFWVHFN
Sbjct: 421 QLKTTQAPQVIKFPQGLNSMVTTTVKRPNKLMNEKEKSEKDEVLVIEGIELDKRFWVHFN 480

Query: 486 VLVNVVDDGSDVCPGNTEFVGTFTTVPHGHNHRGKTSLRVVITEVLKELGIEDDESVIVR 545
           VLVNVVDDGSDVCPGNTEFVGTFTTVPHGHNHRGKTSLRVVITEVLKELGIEDDESVIVR
Sbjct: 481 VLVNVVDDGSDVCPGNTEFVGTFTTVPHGHNHRGKTSLRVVITEVLKELGIEDDESVIVR 540

Query: 546 LDPKVGGDKVTIGGMKIEHQPRG 569
           LDPKVGGDKVTIGGMKIE QP+G
Sbjct: 541 LDPKVGGDKVTIGGMKIELQPKG 563

BLAST of Carg14643 vs. NCBI nr
Match: XP_023542623.1 (polyphenol oxidase, chloroplastic-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 1139.0 bits (2945), Expect = 0.0e+00
Identity = 541/564 (95.92%), Postives = 552/564 (97.87%), Query Frame = 0

Query: 6   MASISPLLSILPYSQIKNQTSNLQNKSRGINHVPCRASNGDSTSKFDRRDVLFGLGGLYG 65
           MASI PLLS++P   IKNQTS LQNKSRGI+HVPCRASNG+STSKFDRRDVLFGLGGLYG
Sbjct: 1   MASIYPLLSVIP---IKNQTSKLQNKSRGISHVPCRASNGESTSKFDRRDVLFGLGGLYG 60

Query: 66  AAALTNQQSPASAAPLAPDVNNCEPATIEGNQHIPCCPPKIHDIIDFIHPPHTTKLRIRP 125
           AAALTNQQSPASAAPLAPDVNNCEPATIEGNQHIPCCPPKIH IIDFIHPPHTTKLRIRP
Sbjct: 61  AAALTNQQSPASAAPLAPDVNNCEPATIEGNQHIPCCPPKIHHIIDFIHPPHTTKLRIRP 120

Query: 126 PAHSLTPKQQFNFEKAIHIMKRLPPNDPRNFYRQADVHCAYCDYAYKQLGFPNAEMQVHW 185
           PAHSLTPKQQFNFEKAIHIMKRLPPNDPRNFYRQADVHCAYCDYAYKQLGFPNAEMQVHW
Sbjct: 121 PAHSLTPKQQFNFEKAIHIMKRLPPNDPRNFYRQADVHCAYCDYAYKQLGFPNAEMQVHW 180

Query: 186 NALFFPFHRAYLYFFERILGHLINEPNFVIPFWNWDNTEGMQMPEIYDHPNSPLYDKLRN 245
           NALFFPFHRAYLYFFERILGHLINEPNFVIPFWNWDN EGMQMP+IYD+PNSPLYDKLRN
Sbjct: 181 NALFFPFHRAYLYFFERILGHLINEPNFVIPFWNWDNNEGMQMPKIYDNPNSPLYDKLRN 240

Query: 246 QRHRPHKLLNLNYHGDDNPSYDVVDSNLRWMHKQMITDANTAEGFHGKLLIASHEANEET 305
            RHRPHKLLNLNYHGDDNPSYDVVD NLRWMHKQMITDA+TAEGFHGKLLIASHEANEET
Sbjct: 241 PRHRPHKLLNLNYHGDDNPSYDVVDCNLRWMHKQMITDADTAEGFHGKLLIASHEANEET 300

Query: 306 GMGTIERSPHNNIHNWVGDPNAKNHMNMGAFYSAARDPLFYGHHANVDRMWSIWQGLKNG 365
           GMGTIERSPHNNIHNWVGDPNAKNHMNMGAFYSAARDPLFYGHHANVDRMWSIWQGLKNG
Sbjct: 301 GMGTIERSPHNNIHNWVGDPNAKNHMNMGAFYSAARDPLFYGHHANVDRMWSIWQGLKNG 360

Query: 366 KPRDFDNPDWLNSSFVFYNEHKQAVRIKVSDCLDTRRLGYVYQYVDLPWLHTKQTPRLKK 425
           KPRDFDNPDWLNSSFVFYNEHKQAVRIKVSDCLDTRRLGYVYQYVDLPWLHTKQTPRLKK
Sbjct: 361 KPRDFDNPDWLNSSFVFYNEHKQAVRIKVSDCLDTRRLGYVYQYVDLPWLHTKQTPRLKK 420

Query: 426 QLKTTQAPQVIKFPQGLNSMVTTTVKRPNKLMNEKEKSEKDEVLVIEGIELDKRFWVHFN 485
           QLK TQAPQVIKFPQGLNSMVTTTVKRPNKL +EKEKSEKDEVLVIEGIELDKR+WVHFN
Sbjct: 421 QLKATQAPQVIKFPQGLNSMVTTTVKRPNKLRDEKEKSEKDEVLVIEGIELDKRYWVHFN 480

Query: 486 VLVNVVDDGSDVCPGNTEFVGTFTTVPHGHNHRGKTSLRVVITEVLKELGIEDDESVIVR 545
           VLVNVVDDGSDVCPGNTEFVGTFTTVPHGHNHRGKTSLRVVITEVLKELGIEDDESVIVR
Sbjct: 481 VLVNVVDDGSDVCPGNTEFVGTFTTVPHGHNHRGKTSLRVVITEVLKELGIEDDESVIVR 540

Query: 546 LDPKVGGDKVTIGGMKIEHQPRGQ 570
           LDPKVGGDKVTIGGMKIE QP+G+
Sbjct: 541 LDPKVGGDKVTIGGMKIELQPKGR 561

BLAST of Carg14643 vs. NCBI nr
Match: XP_022994369.1 (polyphenol oxidase, chloroplastic-like [Cucurbita maxima])

HSP 1 Score: 1135.2 bits (2935), Expect = 0.0e+00
Identity = 539/564 (95.57%), Postives = 549/564 (97.34%), Query Frame = 0

Query: 6   MASISPLLSILPYSQIKNQTSNLQNKSRGINHVPCRASNGDSTSKFDRRDVLFGLGGLYG 65
           MASISPLLSILPYSQIKNQTSN QNKSRGINHVPCRASNGDSTSKFDRRDVLFGLGGLYG
Sbjct: 1   MASISPLLSILPYSQIKNQTSNRQNKSRGINHVPCRASNGDSTSKFDRRDVLFGLGGLYG 60

Query: 66  AAALTNQQSPASAAPLAPDVNNCEPATIEGNQHIPCCPPKIHDIIDFIHPPHTTKLRIRP 125
           AAALTNQQSPASA PLAPDVNNCEPATI GN+HIPCCPPKIHDIIDFIHPPHTTKLRIR 
Sbjct: 61  AAALTNQQSPASAKPLAPDVNNCEPATIVGNEHIPCCPPKIHDIIDFIHPPHTTKLRIRQ 120

Query: 126 PAHSLTPKQQFNFEKAIHIMKRLPPNDPRNFYRQADVHCAYCDYAYKQLGFPNAEMQVHW 185
           PAHSLTPKQQFNFEKAIHIMKRL PNDPRNFYRQADVHCAYCDYAYKQLGFPNAEMQVHW
Sbjct: 121 PAHSLTPKQQFNFEKAIHIMKRLSPNDPRNFYRQADVHCAYCDYAYKQLGFPNAEMQVHW 180

Query: 186 NALFFPFHRAYLYFFERILGHLINEPNFVIPFWNWDNTEGMQMPEIYDHPNSPLYDKLRN 245
           NALFFPFHRAYLYFFERILGHLINEP+F IPFWNWDN +GMQMPEIYDHPNSPLYDKLRN
Sbjct: 181 NALFFPFHRAYLYFFERILGHLINEPDFAIPFWNWDNKKGMQMPEIYDHPNSPLYDKLRN 240

Query: 246 QRHRPHKLLNLNYHGDDNPSYDVVDSNLRWMHKQMITDANTAEGFHGKLLIASHEANEET 305
           QRHRPHKLLNLNYHGDDNP YDVVD NLRWMHKQMITDA+TAEGFHGKLL+AS EANEET
Sbjct: 241 QRHRPHKLLNLNYHGDDNPPYDVVDCNLRWMHKQMITDADTAEGFHGKLLVASQEANEET 300

Query: 306 GMGTIERSPHNNIHNWVGDPNAKNHMNMGAFYSAARDPLFYGHHANVDRMWSIWQGLKNG 365
           GMGTIERSPHNNIHNWVGDPNAKN+MNMG FYSAARDPLFYGHHANVDRMWSIWQGLKNG
Sbjct: 301 GMGTIERSPHNNIHNWVGDPNAKNNMNMGVFYSAARDPLFYGHHANVDRMWSIWQGLKNG 360

Query: 366 KPRDFDNPDWLNSSFVFYNEHKQAVRIKVSDCLDTRRLGYVYQYVDLPWLHTKQTPRLKK 425
           KPRDF+NPDWLNSSFVFYNEHKQAVRIKVSDCLDTRRLGYVYQYVDLPWLHTKQTPRLKK
Sbjct: 361 KPRDFNNPDWLNSSFVFYNEHKQAVRIKVSDCLDTRRLGYVYQYVDLPWLHTKQTPRLKK 420

Query: 426 QLKTTQAPQVIKFPQGLNSMVTTTVKRPNKLMNEKEKSEKDEVLVIEGIELDKRFWVHFN 485
           QLKTTQAPQVIKFPQGLNSMVTTTVKRP KL +EKEKSEKDEVLVIEGIELDKR+WVHFN
Sbjct: 421 QLKTTQAPQVIKFPQGLNSMVTTTVKRPKKLRDEKEKSEKDEVLVIEGIELDKRYWVHFN 480

Query: 486 VLVNVVDDGSDVCPGNTEFVGTFTTVPHGHNHRGKTSLRVVITEVLKELGIEDDESVIVR 545
           VLVNVVDDGSDVCPGNTEFVGTFTTV HGHNHRGKTSLRVVITEVLKELGIEDDESVIVR
Sbjct: 481 VLVNVVDDGSDVCPGNTEFVGTFTTVLHGHNHRGKTSLRVVITEVLKELGIEDDESVIVR 540

Query: 546 LDPKVGGDKVTIGGMKIEHQPRGQ 570
           LDPKVGGDKVTIGGMKIE QP+GQ
Sbjct: 541 LDPKVGGDKVTIGGMKIELQPKGQ 564

BLAST of Carg14643 vs. ExPASy Swiss-Prot
Match: P43309 (Polyphenol oxidase, chloroplastic OS=Malus domestica OX=3750 PE=1 SV=1)

HSP 1 Score: 503.4 bits (1295), Expect = 3.3e-141
Identity = 278/575 (48.35%), Postives = 371/575 (64.52%), Query Frame = 0

Query: 17  PYSQIKNQTSNLQNKSRGI-NHVPCRASNGD----STSKFDRRDVLFGLGGLYGAAALTN 76
           P+SQ  +Q S L    R     V C+A+N D    + SK DRR+VL GLGGLYG A +  
Sbjct: 24  PFSQNNSQVSLLTKPKRSFARKVSCKATNNDQNDQAQSKLDRRNVLLGLGGLYGVAGMGT 83

Query: 77  QQSPASAAPLA-PDVNNCEPATI-EGNQHIPCCPPKIHDIIDFIHPPHTTKLRIRPPAHS 136
               A A P+A PDV+ C PA + +G     CCPP    IIDF   P   KLRIRPPAH+
Sbjct: 84  DPF-AFAKPIAPPDVSKCGPADLPQGAVPTNCCPPPSTKIIDF-KLPAPAKLRIRPPAHA 143

Query: 137 LTPKQQFNFEKAIHIMKRLPPNDPRNFYRQADVHCAYCDYAYKQLGFPNAEMQVHWNALF 196
           +    +  + KA+ +MK LP +DPR+F +QA VHCAYCD AY Q+GFP  E+Q+H + LF
Sbjct: 144 VDQAYRDKYYKAMELMKALPDDDPRSFKQQAAVHCAYCDGAYDQVGFPELELQIHNSWLF 203

Query: 197 FPFHRAYLYFFERILGHLINEPNFVIPFWNWDNTEGMQMPEIYDHPNSPLYDKLRNQRHR 256
           FPFHR YLYFFE+ILG LIN+P F +PFWNWD+  GM +P IY  P SPLYDKLR+  H+
Sbjct: 204 FPFHRYYLYFFEKILGKLINDPTFALPFWNWDSPAGMPLPAIYADPKSPLYDKLRSANHQ 263

Query: 257 PHKLLNLNYHG-DDNPSYD-VVDSNLRWMHKQMITDANTAEGFHGKLLIASHEANEETGM 316
           P  L++L+Y+G +DN S +  +++NL+ M++QM++++  A+ F G    A  E   + G 
Sbjct: 264 PPTLVDLDYNGTEDNVSKETTINANLKIMYRQMVSNSKNAKLFFGNPYRAGDE--PDPGG 323

Query: 317 GTIERSPHNNIHNWVGDPNAKNHMNMGAFYSAARDPLFYGHHANVDRMWSIWQGLKNGKP 376
           G+IE +PH  +H W GD    N  +MG FYSA RDP+F+ HH+NVDRMWSIW+ L  GK 
Sbjct: 324 GSIEGTPHAPVHLWTGDNTQPNFEDMGNFYSAGRDPIFFAHHSNVDRMWSIWKTL-GGKR 383

Query: 377 RDFDNPDWLNSSFVFYNEHKQAVRIKVSDCLDTRRLGYVYQYVDLPWLHTKQTPRLKKQL 436
            D  + DWL+S F+FYNE+ + VR+KV DCL+T+ LGYVYQ VD+PWL +K TPR  K  
Sbjct: 384 TDLTDSDWLDSGFLFYNENAELVRVKVRDCLETKNLGYVYQDVDIPWLSSKPTPRRAKVA 443

Query: 437 KTTQAPQV----------------IKFPQGLNSMVTTTVKRP-NKLMNEKEKSEKDEVLV 496
            +  A ++                 +FP  L S ++T VKRP  K  ++K K +++E+LV
Sbjct: 444 LSKVAKKLGVAHAAVASSSKVVAGTEFPISLGSKISTVVKRPKQKKRSKKAKEDEEEILV 503

Query: 497 IEGIELDKRFWVHFNVLVNVVDDGSDVCPGNTEFVGTFTTVPHGHNHRGK--TSLRVVIT 556
           IEGIE D+   V F+V VN VDD     P  TEF G+F +VPH H H+ K  T LR+ +T
Sbjct: 504 IEGIEFDRDVAVKFDVYVNDVDD-LPSGPDKTEFAGSFVSVPHSHKHKKKMNTILRLGLT 563

Query: 557 EVLKELGIEDDESVIVRLDPKVGGDKVTIGGMKIE 564
           ++L+E+  EDD+SV+V L PK G   V IGG+KIE
Sbjct: 564 DLLEEIEAEDDDSVVVTLVPKFGA--VKIGGIKIE 590

BLAST of Carg14643 vs. ExPASy Swiss-Prot
Match: Q6UIL3 ((+)-larreatricin hydroxylase, chloroplastic OS=Larrea tridentata OX=66636 PE=1 SV=1)

HSP 1 Score: 478.0 bits (1229), Expect = 1.5e-133
Identity = 252/555 (45.41%), Postives = 347/555 (62.52%), Query Frame = 0

Query: 35  INHVPCRASNG--------DSTSKFDRRDVLFGLGGLYGAAALTNQQSPASAAPLAPDV- 94
           +  V C+ASN         + T   DRR++L GLGGLYGA+ +        AAP+ P V 
Sbjct: 28  LRRVSCKASNDNKDKPNDQEKTFSIDRRNMLIGLGGLYGASNVFPSNQSTLAAPIQPPVL 87

Query: 95  -NNCE-PATIEGNQHIPCCPPKIHDIIDFIHPPHTTKLRIRPPAHSLTPKQQFNFEKAIH 154
            ++C  P  +    ++ CCPP + D IDF  P + ++LRIRP AH   P     ++KA+ 
Sbjct: 88  PDSCHPPEDLAEGVNVLCCPPDVKDPIDFQMPSNPSRLRIRPAAHLADPTYIEKYKKALA 147

Query: 155 IMKRLPPNDPRNFYRQADVHCAYCDYAYKQLGFPNAEMQVHWNALFFPFHRAYLYFFERI 214
            MK LP NDPR+FY+QA++HCAYC+ AY Q+GFP+  +QVH + LF PFHR YLYF+ERI
Sbjct: 148 AMKALPQNDPRSFYQQANIHCAYCNGAYDQVGFPDVNIQVHHSWLFLPFHRWYLYFYERI 207

Query: 215 LGHLINEPNFVIPFWNWDNTEGMQMPEIYDHPNSPLYDKLRNQRHRPHKLLNLNYHGDDN 274
           LG LI++P F IPFWNWD  +GM MP ++  PNSPLYD  RN  H P  +++L++   + 
Sbjct: 208 LGSLIDDPTFAIPFWNWDAPKGMHMPHMFIDPNSPLYDAKRNPAHFPDTIVDLDFSSGEA 267

Query: 275 PSYD--VVDSNLRWMHKQMITDANTAEGFHGKLLIASHEANEETGMGTIERSPHNNIHNW 334
           PS++   + +NL  M+KQ++  A  A  FHG+ L A     +E+G G++E +PH NIH W
Sbjct: 268 PSHNPRQIGNNLSIMYKQVVR-AKKARLFHGRPLQAG-SFPDESGDGSLEGTPHGNIHLW 327

Query: 335 VGDPNAKNHMNMGAFYSAARDPLFYGHHANVDRMWSIWQGLKNGKPRDFDNPDWLNSSFV 394
            GDP   N  NMG FYSA RDPLFY HHANVDRMW IW+     K  D+   DWLN+ F+
Sbjct: 328 SGDPRQSNFENMGNFYSAGRDPLFYAHHANVDRMWYIWKNSLGRK--DYKKKDWLNAGFL 387

Query: 395 FYNEHKQAVRIKVSDCLDTRRLGYVYQYVDLPWLHTKQTPRLK-------KQLKTTQAPQ 454
            ++E+ Q VR+ V D LD R+LGY YQ VD+PW+++K  PR         +   TT    
Sbjct: 388 LFDENAQPVRVYVRDALDERKLGYAYQEVDIPWINSKPKPRKANPWPNRLRSKATTTTKL 447

Query: 455 VIKFPQGLNSMVTTTVKRPNKLMNEKEKSEKDEVLVIEGIELDKRFWVHFNVLVNVVDDG 514
           + KFP  L+S V+  VKRP K  ++ EK +++EVLVIE I+ + +F + F+V +N  D+ 
Sbjct: 448 INKFPLTLDSTVSFEVKRPKKSRSKSEKEDEEEVLVIEKIKHEPQFPLKFDVYINDEDED 507

Query: 515 SDVCPGNTEFVGTFTTVPHGHNH------RGKTSLRVVITEVLKELGIEDDESVIVRLDP 564
                  TEF G+F  VPH H H      R  T+L + I+EVL EL ++ D+S++V L P
Sbjct: 508 PSAA-DQTEFAGSFVNVPHFHRHGDKKDKRQTTNLSIGISEVLDELDVDGDDSIVVTLVP 567

BLAST of Carg14643 vs. ExPASy Swiss-Prot
Match: P43311 (Polyphenol oxidase, chloroplastic OS=Vitis vinifera OX=29760 PE=1 SV=1)

HSP 1 Score: 464.5 bits (1194), Expect = 1.7e-129
Identity = 257/588 (43.71%), Postives = 365/588 (62.07%), Query Frame = 0

Query: 13  LSILPYSQIKNQTSNL---QNKSRGI--NHVPCRASNGD------------STSKFDRRD 72
           +S  P S +  + S++   +N+SR    + V C ++NGD            S+ K DRR+
Sbjct: 20  ISAFPPSPLFQRASHVPVARNRSRRFAPSKVSCNSANGDPNSDSTSDVRETSSGKLDRRN 79

Query: 73  VLFGLGGLYGAAALTNQQSP-ASAAPL-APDVNNCEPATI-EGNQHIPCCPPKIHDIIDF 132
           VL G+GGLYGAA       P A  AP+ APD++ C  AT+ +G     CCPP    IIDF
Sbjct: 80  VLLGIGGLYGAAGGLGATKPLAFGAPIQAPDISKCGTATVPDGVTPTNCCPPVTTKIIDF 139

Query: 133 IHPPHTTKLRIRPPAHSLTPKQQFNFEKAIHIMKRLPPNDPRNFYRQADVHCAYCDYAYK 192
             P   + +R RP AH ++ +    ++KAI + K LP +DPR+F +QA+VHC YC  AY 
Sbjct: 140 QLPSSGSPMRTRPAAHLVSKEYLAKYKKAIELQKALPDDDPRSFKQQANVHCTYCQGAYD 199

Query: 193 QLGFPNAEMQVHWNALFFPFHRAYLYFFERILGHLINEPNFVIPFWNWDNTEGMQMPEIY 252
           Q+G+ + E+QVH + LF PFHR YLYF ERIL  LI++P F +P+W WDN +GM MP IY
Sbjct: 200 QVGYTDLELQVHASWLFLPFHRYYLYFNERILAKLIDDPTFALPYWAWDNPDGMYMPTIY 259

Query: 253 DHPNSPLYDKLRNQRHRPHKLLNLNYHGDDN--PSYDVVDSNLRWMHKQMITDANTAEGF 312
               S LYD+ RN +H P  +++L+Y G +   P  ++   NL  M+KQ+++ A T + F
Sbjct: 260 ASSPSSLYDEKRNAKHLPPTVIDLDYDGTEPTIPDDELKTDNLAIMYKQIVSGATTPKLF 319

Query: 313 HGKLLIASHEANEETGMGTIERSPHNNIHNWVGDPNAKNHMNMGAFYSAARDPLFYGHHA 372
            G    A      + G GT+E +PHN +H W G  + K   +MG FY+A RDP+F+GHHA
Sbjct: 320 LGYPYRAGDAI--DPGAGTLEHAPHNIVHKWTGLAD-KPSEDMGNFYTAGRDPIFFGHHA 379

Query: 373 NVDRMWSIWQGLKNGKPRDFDNPDWLNSSFVFYNEHKQAVRIKVSDCLDTRRLGYVYQYV 432
           NVDRMW+IW+ +     +DF + DWL+++FVFY+E+KQ V++KVSDC+DT +L Y YQ +
Sbjct: 380 NVDRMWNIWKTIGGKNRKDFTDTDWLDATFVFYDENKQLVKVKVSDCVDTSKLRYQYQDI 439

Query: 433 DLPWL------HTKQTPRLKK-------QLKTTQAPQVIKFPQGLNSMVTTTVKRPNKLM 492
            +PWL        K T +  K       +L  T    +  FP+ LNS++   V RP K  
Sbjct: 440 PIPWLPKNTKAKAKTTTKSSKSGVAKAAELPKTTISSIGDFPKALNSVIRVEVPRPKKSR 499

Query: 493 NEKEKSEKDEVLVIEGIELDKRFWVHFNVLVNVVDDGSDVCPGNTEFVGTFTTVPHGH-- 552
           ++KEK +++EVL+I+GIELD+  +V F+V +N  +D S   P N+EF G+F  VPH H  
Sbjct: 500 SKKEKEDEEEVLLIKGIELDRENFVKFDVYIN-DEDYSVSRPKNSEFAGSFVNVPHKHMK 559

Query: 553 NHRGKTSLRVVITEVLKELGIEDDESVIVRLDPKVGGDKVTIGGMKIE 564
             + KT+LR  I E+L++LG EDDESVIV + P+ GGD VTIGG++IE
Sbjct: 560 EMKTKTNLRFAINELLEDLGAEDDESVIVTIVPRAGGDDVTIGGIEIE 603

BLAST of Carg14643 vs. ExPASy Swiss-Prot
Match: Q06215 (Polyphenol oxidase A1, chloroplastic OS=Vicia faba OX=3906 PE=1 SV=1)

HSP 1 Score: 452.2 bits (1162), Expect = 8.6e-126
Identity = 256/590 (43.39%), Postives = 355/590 (60.17%), Query Frame = 0

Query: 14  SILPYSQIKNQTSNLQNKSRGINHVPCRASNGDSTSKFD---------RRDVLFGLGGLY 73
           S+ P+ Q ++Q+S L+   R    V C ++N  +  K +         RR+VL GLGG+Y
Sbjct: 25  SVYPFLQKQHQSSKLRKPKR---QVTCSSNNNQNNPKEEQELSNIVGHRRNVLIGLGGIY 84

Query: 74  GAAALTNQQSPASAAPLA-PDVNNCEPATIEGN----QHIPCCPPKIHDIIDFIHPPHTT 133
           G  A TN    A A+P++ PD++ C P +   +     +I CCPP    I DF  P +  
Sbjct: 85  GTLA-TNPS--ALASPISPPDLSKCVPPSDLPSGTTPPNINCCPPYSTKITDFKFPSN-Q 144

Query: 134 KLRIRPPAHSLTPKQQFNFEKAIHIMKRLPPNDPRNFYRQADVHCAYCDYAYKQLGFPNA 193
            LR+R  AH +  +    ++KA  +MK LP NDPRNF +QA++HCAYCD AY Q+GFP+ 
Sbjct: 145 PLRVRQAAHLVDNEFLEKYKKATELMKALPSNDPRNFTQQANIHCAYCDGAYSQIGFPDL 204

Query: 194 EMQVHWNALFFPFHRAYLYFFERILGHLINEPNFVIPFWNWDNTEGMQMPEIYDHPNSPL 253
           ++QVH + LFFPFHR YLYF+ERILG LIN+P F +PFWN+D  +GMQ+P IY    SPL
Sbjct: 205 KLQVHGSWLFFPFHRWYLYFYERILGSLINDPTFALPFWNYDAPDGMQLPTIYADKASPL 264

Query: 254 YDKLRNQRHRPHKLLNLNY--HGDDNPSYDVVDSNLRWMHKQMITDANTAEGFHGKLLIA 313
           YD+LRN  H+P  L++LN+   G D    +++ +NL  M++Q+ ++  T+  F G    A
Sbjct: 265 YDELRNASHQPPTLIDLNFCDIGSDIDRNELIKTNLSIMYRQVYSNGKTSRLFLGNPYRA 324

Query: 314 SHEANEETGMGTIERSPHNNIHNWVGDPNAKNHMNMGAFYSAARDPLFYGHHANVDRMWS 373
                E  G G+IE  PH  +H W GD    N  +MG FYSAARDP+FY HH+NVDR+W 
Sbjct: 325 GDA--EPQGAGSIENVPHAPVHTWTGDNTQTNIEDMGIFYSAARDPIFYSHHSNVDRLWY 384

Query: 374 IWQGLKNGKPRDFDNPDWLNSSFVFYNEHKQAVRIKVSDCLDTRRLGYVYQYVDLPWLHT 433
           IW+ L  GK  DF + DWL S F+FY+E+K  VR+ V D LD  +LGY YQ V +PW   
Sbjct: 385 IWKTL-GGKKHDFTDKDWLESGFLFYDENKNLVRVNVKDSLDIDKLGYAYQDVPIPWEKA 444

Query: 434 KQTPRLKKQLKTT--------------------QAP-QVIKFPQGLNSMVTTTVKRPNKL 493
           K  PR  K  K                      Q+P + + FP  LN+ V+  VKRP KL
Sbjct: 445 KPVPRRTKVQKLVEVEVNDGNLRKSPTIFLVRQQSPRKYVTFPLVLNNKVSAIVKRPKKL 504

Query: 494 MNEKEKSEKDEVLVIEGIELDKRFWVHFNVLVNVVDDGSDVCPGNTEFVGTFTTVPH-GH 553
            ++KEK E++EVLVIEGIE      + F+V +N  D+   V  GNTEF G+F  +PH  H
Sbjct: 505 RSKKEKEEEEEVLVIEGIEFYMNIAIKFDVYIN--DEDDKVGAGNTEFAGSFVNIPHSAH 564

Query: 554 NHRGK---TSLRVVITEVLKELGIEDDESVIVRLDPKVGGDKVTIGGMKI 563
            H+ K   TSLR+ IT++L++L +E D++++V L PK G  +V I  ++I
Sbjct: 565 GHKNKKIITSLRLGITDLLEDLHVEGDDNIVVTLVPKCGSGQVKINNVEI 602

BLAST of Carg14643 vs. ExPASy Swiss-Prot
Match: Q9MB14 (Polyphenol oxidase II, chloroplastic OS=Ipomoea batatas OX=4120 GN=co-2 PE=2 SV=2)

HSP 1 Score: 451.1 bits (1159), Expect = 1.9e-125
Identity = 257/578 (44.46%), Postives = 360/578 (62.28%), Query Frame = 0

Query: 4   KTMASISPLLSILPY-SQIKNQTSNLQNKSRGINHVPCRASNGDSTSKFDRRDVLFGLGG 63
           K+++S + + S LP  SQI   T+   +  +    V C A NGDS  K DRRDVL GLGG
Sbjct: 18  KSLSSSATISSPLPKPSQIHIATAKRTHHFK----VSCNAPNGDSQPKLDRRDVLLGLGG 77

Query: 64  LYGAAALTNQQSPASAAPLAPDVNNC-------EPATIEGNQHIPCCPPKIHDIIDFIHP 123
           L GAA+L N     +    AP+++ C        P  I  N    CCPP   ++I +  P
Sbjct: 78  LAGAASLINNPLAFAEPIHAPEISKCVVPPKDLPPDAIVDN----CCPPLATNVIPYKVP 137

Query: 124 PHT-TKLRIRPPAHSLTPKQQFNFEKAIHIMKRLPPNDPRNFYRQADVHCAYCDYAYKQL 183
             + + ++IRP  H +  +    FEKAI +MK LP +DPRNFY+QA VHCAYC+  Y Q 
Sbjct: 138 KTSPSAMKIRPAIHRMDKEYIAKFEKAIRLMKELPADDPRNFYQQALVHCAYCNGGYVQT 197

Query: 184 GFPNAEMQVHWNALFFPFHRAYLYFFERILGHLINEPNFVIPFWNWDNTEGMQMPEIYDH 243
            +P+ E+QVH + LFFPFHR YLYF+ERILG LI +P F +PFWNWD   GM +P+ + +
Sbjct: 198 DYPDKEIQVHNSWLFFPFHRWYLYFYERILGKLIGDPTFGLPFWNWDTPAGMLIPQYFRN 257

Query: 244 PNSPLYDKLRNQRHRPHKLLNLNYHGDDNPSYDV--VDSNLRWMHKQMITDANTAEGFHG 303
            NSPLYD+ RNQ H P  +++L Y G D    D   + +NL  M+K M+T+A TAE F G
Sbjct: 258 QNSPLYDENRNQSHLP-LVMDLGYAGTDTDVTDQERISNNLALMYKSMVTNAGTAELFLG 317

Query: 304 KLLIASHEANEETGMGTIERSPHNNIHNWVGD--PNAKNHMNMGAFYSAARDPLFYGHHA 363
           K   A  +   + G G+IE  PH  +H WVGD  P  +N  +MG FYSA RD LFY HH+
Sbjct: 318 KPYKAGDDPVNKGG-GSIENIPHTPVHRWVGDVKPRTQNGEDMGNFYSAGRDILFYCHHS 377

Query: 364 NVDRMWSIWQGL-KNGKPRDFDNPDWLNSSFVFYNEHKQAVRIKVSDCLDTRRLGYVYQY 423
           NVDRMW+IWQ L   G+ RDF + DWL+++F+FY+E+KQAVR++V D LD ++LGY Y++
Sbjct: 378 NVDRMWTIWQQLGGKGRRRDFTDSDWLDATFIFYDENKQAVRVRVGDALDNQKLGYKYEF 437

Query: 424 VDLPWLHTKQTPRLKKQ--LKTTQAPQVIK-FPQGLNSMVTTTVKRPNKLMNEKEKSEKD 483
            +LPWL++K  P  KK      ++AP V   FP  L+ +V   V RP K  +++EK  ++
Sbjct: 438 TNLPWLNSKPLPTKKKTGLAARSKAPFVTDVFPLTLDKVVQVKVPRPKKSRSKEEKEAEE 497

Query: 484 EVLVIEGIELDKRFWVHFNVLVNVVDDGSDVCPGNTEFVGTFTTVPHGH--NHRGKTSLR 543
           E+L I+GIE+    +  F+V +N  +D  +      E+ G+F  +PH H  + + +TSL 
Sbjct: 498 EILEIQGIEVAIDQYAKFDVYLN-DEDEPEAGKEKAEYAGSFAHLPHKHTGSKKIRTSLS 557

Query: 544 VVITEVLKELGIEDDESVIVRLDPKVGGDKVTIGGMKI 563
           + + E L++LG EDD++V+V L PKVGG  VT+  +KI
Sbjct: 558 LGLNEPLEDLGAEDDDAVLVTLAPKVGGGVVTVENIKI 584

BLAST of Carg14643 vs. ExPASy TrEMBL
Match: A0A6J1GRK5 (polyphenol oxidase, chloroplastic-like OS=Cucurbita moschata OX=3662 GN=LOC111456826 PE=3 SV=1)

HSP 1 Score: 1170.2 bits (3026), Expect = 0.0e+00
Identity = 556/564 (98.58%), Postives = 559/564 (99.11%), Query Frame = 0

Query: 6   MASISPLLSILPYSQIKNQTSNLQNKSRGINHVPCRASNGDSTSKFDRRDVLFGLGGLYG 65
           MASISPLLSILPYSQIKNQTSNLQNKSRGIN VPCRASNGDSTSKFDRRDVLFGLGGLYG
Sbjct: 1   MASISPLLSILPYSQIKNQTSNLQNKSRGINQVPCRASNGDSTSKFDRRDVLFGLGGLYG 60

Query: 66  AAALTNQQSPASAAPLAPDVNNCEPATIEGNQHIPCCPPKIHDIIDFIHPPHTTKLRIRP 125
           AAALTNQQSPASAAPLAPDVNNCE ATIEGN+HIPCCPPKIHDIIDFIHPPHTTKLRIRP
Sbjct: 61  AAALTNQQSPASAAPLAPDVNNCEAATIEGNEHIPCCPPKIHDIIDFIHPPHTTKLRIRP 120

Query: 126 PAHSLTPKQQFNFEKAIHIMKRLPPNDPRNFYRQADVHCAYCDYAYKQLGFPNAEMQVHW 185
           PAHSLTPKQQFNFEKAIHIMKRLPPNDPRNFYRQADVHCAYCDYAYKQLGFPNAEMQVHW
Sbjct: 121 PAHSLTPKQQFNFEKAIHIMKRLPPNDPRNFYRQADVHCAYCDYAYKQLGFPNAEMQVHW 180

Query: 186 NALFFPFHRAYLYFFERILGHLINEPNFVIPFWNWDNTEGMQMPEIYDHPNSPLYDKLRN 245
           NALFFPFHRAYLYFFERILGHLINEPNFVIPFWNWDNTEGMQMPEIYDHPNSPLYDKLRN
Sbjct: 181 NALFFPFHRAYLYFFERILGHLINEPNFVIPFWNWDNTEGMQMPEIYDHPNSPLYDKLRN 240

Query: 246 QRHRPHKLLNLNYHGDDNPSYDVVDSNLRWMHKQMITDANTAEGFHGKLLIASHEANEET 305
           QRHRPHKLLNLNYHGDDNPSYDVVDSNLRWMHKQMITDANTAEGFHGKLLIASHEANEET
Sbjct: 241 QRHRPHKLLNLNYHGDDNPSYDVVDSNLRWMHKQMITDANTAEGFHGKLLIASHEANEET 300

Query: 306 GMGTIERSPHNNIHNWVGDPNAKNHMNMGAFYSAARDPLFYGHHANVDRMWSIWQGLKNG 365
           GMGTIE+SPHNNIHNWVGDPNAKNHMNMGAFYSAARDPLFYGHHANVDRMWSIWQGLKNG
Sbjct: 301 GMGTIEKSPHNNIHNWVGDPNAKNHMNMGAFYSAARDPLFYGHHANVDRMWSIWQGLKNG 360

Query: 366 KPRDFDNPDWLNSSFVFYNEHKQAVRIKVSDCLDTRRLGYVYQYVDLPWLHTKQTPRLKK 425
           K RDFDNPDWLNSSFVFYNEHKQAVRIKV DCLDTRRLGYVYQYVDLPWLHTKQTPRLKK
Sbjct: 361 KARDFDNPDWLNSSFVFYNEHKQAVRIKVRDCLDTRRLGYVYQYVDLPWLHTKQTPRLKK 420

Query: 426 QLKTTQAPQVIKFPQGLNSMVTTTVKRPNKLMNEKEKSEKDEVLVIEGIELDKRFWVHFN 485
           QLKTTQAPQVIKFPQGLNSMVTTTVKRPNKLMNEKEKSEKDEVLVIEGIELDKRFWVHFN
Sbjct: 421 QLKTTQAPQVIKFPQGLNSMVTTTVKRPNKLMNEKEKSEKDEVLVIEGIELDKRFWVHFN 480

Query: 486 VLVNVVDDGSDVCPGNTEFVGTFTTVPHGHNHRGKTSLRVVITEVLKELGIEDDESVIVR 545
           VLVNVVDDGSDVCPGNTEFVGTFTTVPHGHNHRGKTSLRVVITEVLKELGIEDDESVIVR
Sbjct: 481 VLVNVVDDGSDVCPGNTEFVGTFTTVPHGHNHRGKTSLRVVITEVLKELGIEDDESVIVR 540

Query: 546 LDPKVGGDKVTIGGMKIEHQPRGQ 570
           LDPKVGGDKVTIGGMKIE QP+GQ
Sbjct: 541 LDPKVGGDKVTIGGMKIELQPKGQ 564

BLAST of Carg14643 vs. ExPASy TrEMBL
Match: A0A6J1K2N9 (polyphenol oxidase, chloroplastic-like OS=Cucurbita maxima OX=3661 GN=LOC111490108 PE=3 SV=1)

HSP 1 Score: 1135.2 bits (2935), Expect = 0.0e+00
Identity = 539/564 (95.57%), Postives = 549/564 (97.34%), Query Frame = 0

Query: 6   MASISPLLSILPYSQIKNQTSNLQNKSRGINHVPCRASNGDSTSKFDRRDVLFGLGGLYG 65
           MASISPLLSILPYSQIKNQTSN QNKSRGINHVPCRASNGDSTSKFDRRDVLFGLGGLYG
Sbjct: 1   MASISPLLSILPYSQIKNQTSNRQNKSRGINHVPCRASNGDSTSKFDRRDVLFGLGGLYG 60

Query: 66  AAALTNQQSPASAAPLAPDVNNCEPATIEGNQHIPCCPPKIHDIIDFIHPPHTTKLRIRP 125
           AAALTNQQSPASA PLAPDVNNCEPATI GN+HIPCCPPKIHDIIDFIHPPHTTKLRIR 
Sbjct: 61  AAALTNQQSPASAKPLAPDVNNCEPATIVGNEHIPCCPPKIHDIIDFIHPPHTTKLRIRQ 120

Query: 126 PAHSLTPKQQFNFEKAIHIMKRLPPNDPRNFYRQADVHCAYCDYAYKQLGFPNAEMQVHW 185
           PAHSLTPKQQFNFEKAIHIMKRL PNDPRNFYRQADVHCAYCDYAYKQLGFPNAEMQVHW
Sbjct: 121 PAHSLTPKQQFNFEKAIHIMKRLSPNDPRNFYRQADVHCAYCDYAYKQLGFPNAEMQVHW 180

Query: 186 NALFFPFHRAYLYFFERILGHLINEPNFVIPFWNWDNTEGMQMPEIYDHPNSPLYDKLRN 245
           NALFFPFHRAYLYFFERILGHLINEP+F IPFWNWDN +GMQMPEIYDHPNSPLYDKLRN
Sbjct: 181 NALFFPFHRAYLYFFERILGHLINEPDFAIPFWNWDNKKGMQMPEIYDHPNSPLYDKLRN 240

Query: 246 QRHRPHKLLNLNYHGDDNPSYDVVDSNLRWMHKQMITDANTAEGFHGKLLIASHEANEET 305
           QRHRPHKLLNLNYHGDDNP YDVVD NLRWMHKQMITDA+TAEGFHGKLL+AS EANEET
Sbjct: 241 QRHRPHKLLNLNYHGDDNPPYDVVDCNLRWMHKQMITDADTAEGFHGKLLVASQEANEET 300

Query: 306 GMGTIERSPHNNIHNWVGDPNAKNHMNMGAFYSAARDPLFYGHHANVDRMWSIWQGLKNG 365
           GMGTIERSPHNNIHNWVGDPNAKN+MNMG FYSAARDPLFYGHHANVDRMWSIWQGLKNG
Sbjct: 301 GMGTIERSPHNNIHNWVGDPNAKNNMNMGVFYSAARDPLFYGHHANVDRMWSIWQGLKNG 360

Query: 366 KPRDFDNPDWLNSSFVFYNEHKQAVRIKVSDCLDTRRLGYVYQYVDLPWLHTKQTPRLKK 425
           KPRDF+NPDWLNSSFVFYNEHKQAVRIKVSDCLDTRRLGYVYQYVDLPWLHTKQTPRLKK
Sbjct: 361 KPRDFNNPDWLNSSFVFYNEHKQAVRIKVSDCLDTRRLGYVYQYVDLPWLHTKQTPRLKK 420

Query: 426 QLKTTQAPQVIKFPQGLNSMVTTTVKRPNKLMNEKEKSEKDEVLVIEGIELDKRFWVHFN 485
           QLKTTQAPQVIKFPQGLNSMVTTTVKRP KL +EKEKSEKDEVLVIEGIELDKR+WVHFN
Sbjct: 421 QLKTTQAPQVIKFPQGLNSMVTTTVKRPKKLRDEKEKSEKDEVLVIEGIELDKRYWVHFN 480

Query: 486 VLVNVVDDGSDVCPGNTEFVGTFTTVPHGHNHRGKTSLRVVITEVLKELGIEDDESVIVR 545
           VLVNVVDDGSDVCPGNTEFVGTFTTV HGHNHRGKTSLRVVITEVLKELGIEDDESVIVR
Sbjct: 481 VLVNVVDDGSDVCPGNTEFVGTFTTVLHGHNHRGKTSLRVVITEVLKELGIEDDESVIVR 540

Query: 546 LDPKVGGDKVTIGGMKIEHQPRGQ 570
           LDPKVGGDKVTIGGMKIE QP+GQ
Sbjct: 541 LDPKVGGDKVTIGGMKIELQPKGQ 564

BLAST of Carg14643 vs. ExPASy TrEMBL
Match: A0A172DC01 (Polyphenol oxidase OS=Luffa aegyptiaca OX=3670 GN=PPO2 PE=2 SV=1)

HSP 1 Score: 681.4 bits (1757), Expect = 3.2e-192
Identity = 344/577 (59.62%), Postives = 427/577 (74.00%), Query Frame = 0

Query: 6   MASISPLLSILPYSQIKNQTSNLQNKSRGINHVPCRASN------------GDSTSKFDR 65
           MAS+S LLS+ P  + KNQ+S+  N+  G++++PCRASN            GDS SKFDR
Sbjct: 1   MASLSSLLSLFPCIRSKNQSSSRGNRRLGLSYIPCRASNGVQTPMNSNPQEGDSMSKFDR 60

Query: 66  RDVLFGLGGLYGAAALTNQQSPASAAPLAPDVNNCEPATIEGNQHIPCCPPKIHDIIDFI 125
           RDVLFGLGGLY AAAL   +S A A P++PDV +CEP T+E ++ + CCPP I DI DF+
Sbjct: 61  RDVLFGLGGLYSAAALGRDRS-ALADPISPDVIHCEPITLEADEKVDCCPPSILDITDFV 120

Query: 126 HPPHTTKLRIRPPAHSLTPKQQFNFEKAIHIMKRLPPNDPRNFYRQADVHCAYCDYAYKQ 185
            PP    LR+RP AH +T +Q    E+A+  MK L P+DPRNFY+QA VHCAYC+ AYKQ
Sbjct: 121 -PPAVENLRVRPAAHLITQEQLAKLEEAVTKMKALDPDDPRNFYQQARVHCAYCNDAYKQ 180

Query: 186 LGFPNAEMQVHWNALFFPFHRAYLYFFERILGHLINEPNFVIPFWNWDNTEGMQMPEIY- 245
            GFP  +  +HWN LFFPFHR YLYF ERILG LIN+P+F IPFWNWD   GMQMP IY 
Sbjct: 181 DGFPELDFDIHWNWLFFPFHRCYLYFHERILGSLINDPDFAIPFWNWDAIPGMQMPAIYI 240

Query: 246 -DHPNSPLYDKLRNQRHRPHKLLNLNYHG-DDNPSYDVVDSNLRWMHKQMITDANTAEGF 305
            D  NSPLYD+LR+  H+P KLL+LNY G  ++PSYDV+  N R M++QM+ ++ TAEGF
Sbjct: 241 NDDKNSPLYDELRDPNHKPPKLLDLNYDGATEDPSYDVLFCNPRLMYRQMVINSKTAEGF 300

Query: 306 HGKLLIASHEANEETGMGTIERSPHNNIHNWVGDPNAKNHMNMGAFYSAARDPLFYGHHA 365
           HGK L A  EA   TGMG+IE S HNN+H W GDP  K   +MG FY+AARDPLFY HHA
Sbjct: 301 HGKPLRAGQEA-YRTGMGSIELSAHNNVHTWSGDPQQKYREDMGHFYAAARDPLFYAHHA 360

Query: 366 NVDRMWSIWQGLKNGKPRDFDNPDWLNSSFVFYNEHKQAVRIKVSDCLDTRRLGYVYQYV 425
           NVDRMW+IWQGL +GK RDF +PDWLN+SFVFY+E+K A+RIKV DCLD  +LGY YQ V
Sbjct: 361 NVDRMWTIWQGLIDGKSRDFTDPDWLNASFVFYDENKNAIRIKVQDCLDNEKLGYKYQAV 420

Query: 426 DLPWLHTKQTPRLK-KQLKTTQAPQVIKFPQGLNSMVTTTVKRPNKLMNEKEKSEKDEVL 485
           ++PWLHTK  PR K  QLK+++A +     + LNS +T  VKRP +  ++ +KS +DEVL
Sbjct: 421 EIPWLHTKPIPRQKTSQLKSSKAQETPPI-EVLNSKMTKEVKRPQRSRSKTQKSVEDEVL 480

Query: 486 VIEGIELDKRFWVHFNVLVNVVDDGSDVCPGNTEFVGTFTTVPHGHNHRGKTSLRVVITE 545
           VIE +ELD    VHFNVLVNV+DDG+DV PGNTEF G+F ++P GH HR  TSL +VITE
Sbjct: 481 VIE-MELDTTSRVHFNVLVNVLDDGTDVSPGNTEFAGSFKSIPLGHKHRANTSLSLVITE 540

Query: 546 VLKELGIEDDESVIVRLDPKVGGDKVTIGGMKIEHQP 567
           +++ELG +DDE+V V +DPK+GGD VTIGG+KIE QP
Sbjct: 541 LIEELGYDDDETVFVTIDPKLGGDLVTIGGIKIELQP 572

BLAST of Carg14643 vs. ExPASy TrEMBL
Match: A0A5A7UTU0 (Polyphenol oxidase I OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold861G00530 PE=3 SV=1)

HSP 1 Score: 571.6 bits (1472), Expect = 3.6e-159
Identity = 307/566 (54.24%), Postives = 393/566 (69.43%), Query Frame = 0

Query: 6   MASISPLLSILPYSQIKNQTSNLQ-NKSRGI-NHVPCRASNG--DSTSKFDRRDVLFGLG 65
           MAS+S LLS+ PYS  K Q S +  NK RG+ NHV C+ASN   DS +KFDRRD+LFGLG
Sbjct: 1   MASLSSLLSVSPYSGCKIQPSVIPLNKRRGVNNHVSCKASNNGEDSVNKFDRRDMLFGLG 60

Query: 66  GLYGAAALTNQQ-SPASAAPLAPDVNNCEPATIEG-NQHIPCCPPKIHDIIDFIHPPHTT 125
           GLYG A L+ +  + A    L+PDV+NC+P       + + CCPPKI +II+F  PP   
Sbjct: 61  GLYGVATLSGKDIALADDWSLSPDVHNCKPVKPSAVGKKMDCCPPKIKEIIEFT-PPVVP 120

Query: 126 KLRIRPPAHSLTPKQQFNFEKAIHIMKRLPPNDPRNFYRQADVHCAYCDYAYKQLGFPNA 185
             R+RP  H +TP+Q  + EK I +MK LP +DPR+F  QA VHCAYCD AYKQ+     
Sbjct: 121 TPRVRPAVHLMTPEQIADLEKGIQMMKMLPEDDPRSFAHQAKVHCAYCDDAYKQMD-STM 180

Query: 186 EMQVHWNALFFPFHRAYLYFFERILGHLINEPNFVIPFWNWDNTEGMQMPEIYDHPNSPL 245
           + QVH NALFFPFHRAYLYFFERIL HL+ +P+F IPFWNWD   GM MP+IY  P+S L
Sbjct: 181 DFQVHENALFFPFHRAYLYFFERILCHLLEKPDFAIPFWNWDAPPGMCMPKIYTKPSSSL 240

Query: 246 YDKLRNQRHRPHKLLNLNYHGDDNPSYDVVDSNLRWMHKQMITDANTAEGFHGKLLIASH 305
           YD+ R++ H+P KLLNLNY G +    ++VD NLRWM+ QM     TA+ F GK++   +
Sbjct: 241 YDENRDKCHQPPKLLNLNYGGKEVKDPEIVDFNLRWMNDQM--SVETAKEFLGKVIKRGN 300

Query: 306 EANEETGMGTIERSPHNNIHNWVGDPNAKNH-MNMGAFYSAARDPLFYGHHANVDRMWSI 365
               E G G+IE SPHNNIH WVGDP A  H  +MG FYSA RDPLFY HHANVDRMW+I
Sbjct: 301 --GPENGSGSIETSPHNNIHIWVGDPEATIHGEDMGHFYSAGRDPLFYAHHANVDRMWNI 360

Query: 366 WQGLKNGKPRDFDNPDWLNSSFVFYNEHKQAVRIKVSDCLDTRRLGYVYQYVDLPWLHTK 425
           WQ +K+ +PRD  + DWLNSSFV Y+E+++AVRIKVS CLD++ LGYVYQ V +PW  T 
Sbjct: 361 WQKMKD-RPRDHTDMDWLNSSFVLYDENRKAVRIKVSQCLDSKILGYVYQDVPIPWKDTP 420

Query: 426 QTPRLKKQLKTTQAPQVIKFPQGLNSMVTTTVKRPNKLMNEKEKSEKDEVLVIEGIELDK 485
              R K++      P  I FPQ L+S +TT V RP ++ + +E+  K+EVLV+E +E D 
Sbjct: 421 PIRRPKQERSLGNLPPTIDFPQILSSDITTMVDRP-EVASPEERINKNEVLVLEDVEYDP 480

Query: 486 RFWVHFNVLVNVVDDGSDVCPGNTEFVGTFTTVPHGHNHRGKTSLRVVITEVLKELGIED 545
              +HF+VL+NV D+G DV  G+ EFVGTFT VPHG     KTSL  VIT+ +++LG+E 
Sbjct: 481 TLPLHFDVLLNVGDEGEDVRAGHIEFVGTFTNVPHGRTTYHKTSLHFVITKKIQQLGLEG 540

Query: 546 DESVIVRLDPKV-GGDKVTIGGMKIE 564
           D+ V V++ P V GGD++ IGG+KI+
Sbjct: 541 DKKVAVKIVPNVNGGDRIKIGGIKIQ 558

BLAST of Carg14643 vs. ExPASy TrEMBL
Match: A0A5A7UM24 (Polyphenol oxidase I OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold96G001410 PE=3 SV=1)

HSP 1 Score: 567.8 bits (1462), Expect = 5.2e-158
Identity = 306/566 (54.06%), Postives = 389/566 (68.73%), Query Frame = 0

Query: 6   MASISPLLSILPYSQIKNQTSNLQ-NKSRGI-NHVPCRASNG--DSTSKFDRRDVLFGLG 65
           MAS+S LLS+ PYS  K Q S +  NK RG+ NHV C+ASN   DS +KFDRRD+LFGLG
Sbjct: 1   MASLSSLLSVSPYSGCKIQPSVIPLNKRRGVNNHVSCKASNNGEDSVNKFDRRDMLFGLG 60

Query: 66  GLYGAAALTNQQ-SPASAAPLAPDVNNCEPATIEG-NQHIPCCPPKIHDIIDFIHPPHTT 125
           GLYG A L+ +  + A    L+PDV+NC+P       + + CCPPKI +II+F  PP   
Sbjct: 61  GLYGVATLSGKDIALADDWSLSPDVHNCKPVKPSAVGKKMDCCPPKIKEIIEFT-PPVVP 120

Query: 126 KLRIRPPAHSLTPKQQFNFEKAIHIMKRLPPNDPRNFYRQADVHCAYCDYAYKQLGFPNA 185
             R+RP  H +TP Q  + EK I +MK LP +DPR+F  QA VHCAYCD AYKQ+     
Sbjct: 121 TPRVRPAVHLMTPDQIADLEKGIQMMKMLPEDDPRSFAHQAKVHCAYCDDAYKQMD-STM 180

Query: 186 EMQVHWNALFFPFHRAYLYFFERILGHLINEPNFVIPFWNWDNTEGMQMPEIYDHPNSPL 245
           + QVH NALFFPFHRAYLYFFERIL HL+ +P+F IPFWNWD   GM MP+IY  P+S L
Sbjct: 181 DFQVHENALFFPFHRAYLYFFERILCHLLEKPDFAIPFWNWDAPPGMCMPKIYTKPSSSL 240

Query: 246 YDKLRNQRHRPHKLLNLNYHGDDNPSYDVVDSNLRWMHKQMITDANTAEGFHGKLLIASH 305
           YD+ R++ H+P KLLNLNY G      ++VD NLRWM+ QM     TA  F GK++   +
Sbjct: 241 YDENRDECHQPPKLLNLNYGGKKVKDSEIVDFNLRWMNDQM--SVETAREFLGKVIKRGN 300

Query: 306 EANEETGMGTIERSPHNNIHNWVGDPNAKNH-MNMGAFYSAARDPLFYGHHANVDRMWSI 365
               E G G+IE SPHNN+HNWVGDP A  H  +MG FYSA RDPLFY HHANVDRMW+I
Sbjct: 301 --CPENGSGSIETSPHNNLHNWVGDPEATIHGEDMGHFYSAGRDPLFYAHHANVDRMWNI 360

Query: 366 WQGLKNGKPRDFDNPDWLNSSFVFYNEHKQAVRIKVSDCLDTRRLGYVYQYVDLPWLHTK 425
           WQ +K+   RD  + DWLNSSFV Y+E+++AVRIKVS CLD++ LGYVYQ V +PW  T 
Sbjct: 361 WQNMKD--RRDHTDMDWLNSSFVLYDENRKAVRIKVSQCLDSKILGYVYQDVPIPWKDTP 420

Query: 426 QTPRLKKQLKTTQAPQVIKFPQGLNSMVTTTVKRPNKLMNEKEKSEKDEVLVIEGIELDK 485
              R K++      P  I FPQ L+S +TT V RP ++ + +E+  K+EVLV+E +E D 
Sbjct: 421 PIRRPKQERSLGNLPPTIDFPQILSSDITTMVDRP-EVASPEERINKNEVLVLEDVEYDP 480

Query: 486 RFWVHFNVLVNVVDDGSDVCPGNTEFVGTFTTVPHGHNHRGKTSLRVVITEVLKELGIED 545
              +HF+VL+NV D+G DV  G+ EFVGTFT VPHG     KTSL  VIT+ +++LG+E 
Sbjct: 481 TLPLHFDVLLNVGDEGEDVRAGHIEFVGTFTNVPHGRTTYHKTSLHFVITKKIQQLGLEG 540

Query: 546 DESVIVRLDPKV-GGDKVTIGGMKIE 564
           D+ V V++ P V GGD++ IGG+KI+
Sbjct: 541 DKKVAVKIVPNVNGGDRIKIGGIKIQ 557

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
KAG7012418.10.0e+00100.00hypothetical protein SDJN02_25170, partial [Cucurbita argyrosperma subsp. argyro... [more]
XP_022954613.10.0e+0098.58polyphenol oxidase, chloroplastic-like [Cucurbita moschata][more]
KAG6573251.10.0e+0098.40hypothetical protein SDJN03_27138, partial [Cucurbita argyrosperma subsp. sorori... [more]
XP_023542623.10.0e+0095.92polyphenol oxidase, chloroplastic-like [Cucurbita pepo subsp. pepo][more]
XP_022994369.10.0e+0095.57polyphenol oxidase, chloroplastic-like [Cucurbita maxima][more]
Match NameE-valueIdentityDescription
P433093.3e-14148.35Polyphenol oxidase, chloroplastic OS=Malus domestica OX=3750 PE=1 SV=1[more]
Q6UIL31.5e-13345.41(+)-larreatricin hydroxylase, chloroplastic OS=Larrea tridentata OX=66636 PE=1 S... [more]
P433111.7e-12943.71Polyphenol oxidase, chloroplastic OS=Vitis vinifera OX=29760 PE=1 SV=1[more]
Q062158.6e-12643.39Polyphenol oxidase A1, chloroplastic OS=Vicia faba OX=3906 PE=1 SV=1[more]
Q9MB141.9e-12544.46Polyphenol oxidase II, chloroplastic OS=Ipomoea batatas OX=4120 GN=co-2 PE=2 SV=... [more]
Match NameE-valueIdentityDescription
A0A6J1GRK50.0e+0098.58polyphenol oxidase, chloroplastic-like OS=Cucurbita moschata OX=3662 GN=LOC11145... [more]
A0A6J1K2N90.0e+0095.57polyphenol oxidase, chloroplastic-like OS=Cucurbita maxima OX=3661 GN=LOC1114901... [more]
A0A172DC013.2e-19259.62Polyphenol oxidase OS=Luffa aegyptiaca OX=3670 GN=PPO2 PE=2 SV=1[more]
A0A5A7UTU03.6e-15954.24Polyphenol oxidase I OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold861... [more]
A0A5A7UM245.2e-15854.06Polyphenol oxidase I OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold96G... [more]
Match NameE-valueIdentityDescription
InterPro
Analysis Name: InterPro Annotations of Silver-seed gourd (SMH-JMG-627) v2
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR002227Tyrosinase copper-binding domainPRINTSPR00092TYROSINASEcoord: 341..359
score: 57.04
coord: 184..201
score: 51.36
coord: 314..325
score: 39.97
coord: 215..220
score: 57.82
IPR002227Tyrosinase copper-binding domainPFAMPF00264Tyrosinasecoord: 153..360
e-value: 1.2E-31
score: 110.8
IPR002227Tyrosinase copper-binding domainPROSITEPS00498TYROSINASE_2coord: 342..353
IPR002227Tyrosinase copper-binding domainPROSITEPS00497TYROSINASE_1coord: 184..201
IPR022739Polyphenol oxidase, central domainPFAMPF12142PPO1_DWLcoord: 369..418
e-value: 1.1E-22
score: 79.5
IPR008922Uncharacterised domain, di-copper centreGENE3D1.10.1280.10coord: 80..416
e-value: 1.9E-104
score: 351.8
IPR008922Uncharacterised domain, di-copper centreSUPERFAMILY48056Di-copper centre-containing domaincoord: 97..415
IPR022740Polyphenol oxidase, C-terminalPFAMPF12143PPO1_KFDVcoord: 438..563
e-value: 1.3E-38
score: 132.0
IPR016213Polyphenol oxidasePIRSFPIRSF000290PPO_plantcoord: 2..566
e-value: 7.8E-211
score: 699.8
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 27..46
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 27..47
NoneNo IPR availablePANTHERPTHR11474TYROSINASE FAMILY MEMBERcoord: 13..565
NoneNo IPR availablePANTHERPTHR11474:SF94POLYPHENOL OXIDASE, CHLOROPLASTIC-LIKEcoord: 13..565

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Carg14643-RACarg14643-RAmRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0046148 pigment biosynthetic process
molecular_function GO:0004097 catechol oxidase activity
molecular_function GO:0046872 metal ion binding
molecular_function GO:0016491 oxidoreductase activity