Carg14526 (gene) Silver-seed gourd (SMH-JMG-627) v2

BLAST of Carg14526 vs. NCBI nr
Match: KAG7028098.1 (GDSL esterase/lipase [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 815.5 bits (2105), Expect = 2.0e-232
Identity = 393/393 (100.00%), Postives = 393/393 (100.00%), Query Frame = 0

Query: 1   MDCKKLLCFSLLCFSLLPELYGVVWVSAGGGGHRHRHRVRRSGQSLGGGDLSDSNPRKLF 60
           MDCKKLLCFSLLCFSLLPELYGVVWVSAGGGGHRHRHRVRRSGQSLGGGDLSDSNPRKLF
Sbjct: 1   MDCKKLLCFSLLCFSLLPELYGVVWVSAGGGGHRHRHRVRRSGQSLGGGDLSDSNPRKLF 60

Query: 61  VFGDSYVDTGNNRKPAAKSWQYPYGITFPGKPTGRFSDGRVLTDYLAKYLRVKSPIAYKW 120
           VFGDSYVDTGNNRKPAAKSWQYPYGITFPGKPTGRFSDGRVLTDYLAKYLRVKSPIAYKW
Sbjct: 61  VFGDSYVDTGNNRKPAAKSWQYPYGITFPGKPTGRFSDGRVLTDYLAKYLRVKSPIAYKW 120

Query: 121 RKVGLGQLKYGMNFAYGGTGVFNTFIMSPNMSTQIDFLQQLIHESTYTAQDLHYSVALIS 180
           RKVGLGQLKYGMNFAYGGTGVFNTFIMSPNMSTQIDFLQQLIHESTYTAQDLHYSVALIS
Sbjct: 121 RKVGLGQLKYGMNFAYGGTGVFNTFIMSPNMSTQIDFLQQLIHESTYTAQDLHYSVALIS 180

Query: 181 LAGNDYGVYQATDGSAQSKYKKLSKEFGDFVLSLACWWQGWQPFITKVVNQLEVNLRRIH 240
           LAGNDYGVYQATDGSAQSKYKKLSKEFGDFVLSLACWWQGWQPFITKVVNQLEVNLRRIH
Sbjct: 181 LAGNDYGVYQATDGSAQSKYKKLSKEFGDFVLSLACWWQGWQPFITKVVNQLEVNLRRIH 240

Query: 241 GLGVPKIVVTSLEPLGCLPSSTVASSFQQCNATENLLVNFHNLLLQQSVAKLNSEVKDAS 300
           GLGVPKIVVTSLEPLGCLPSSTVASSFQQCNATENLLVNFHNLLLQQSVAKLNSEVKDAS
Sbjct: 241 GLGVPKIVVTSLEPLGCLPSSTVASSFQQCNATENLLVNFHNLLLQQSVAKLNSEVKDAS 300

Query: 301 ASTFILLDLYSSFMAALNNKEDPLGNVKFENPLKPCCVGISSEYACGNVGANGEKKYTIC 360
           ASTFILLDLYSSFMAALNNKEDPLGNVKFENPLKPCCVGISSEYACGNVGANGEKKYTIC
Sbjct: 301 ASTFILLDLYSSFMAALNNKEDPLGNVKFENPLKPCCVGISSEYACGNVGANGEKKYTIC 360

Query: 361 EDPGAAFFWDEVHPSQNGWFAVYSALQANLKQL 394
           EDPGAAFFWDEVHPSQNGWFAVYSALQANLKQL
Sbjct: 361 EDPGAAFFWDEVHPSQNGWFAVYSALQANLKQL 393

BLAST of Carg14526 vs. NCBI nr
Match: KAG6596562.1 (GDSL esterase/lipase, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 752.3 bits (1941), Expect = 2.1e-213
Identity = 369/393 (93.89%), Postives = 371/393 (94.40%), Query Frame = 0

Query: 1   MDCKKLLCFSLLCFSLLPELYGVVWVSAGGGGHRHRHRVRRSGQSLGGGDLSDSNPRKLF 60
           MDCKKLLCFSLLCFSLLPELYGV+WVSAGGGGHRHRHRVRRSGQSLGGG+LSDSNPRKLF
Sbjct: 1   MDCKKLLCFSLLCFSLLPELYGVIWVSAGGGGHRHRHRVRRSGQSLGGGELSDSNPRKLF 60

Query: 61  VFGDSYVDTGNNRKPAAKSWQYPYGITFPGKPTGRFSDGRVLTDYLAKYLRVKSPIAYKW 120
           VFGDSYVDTGNNRKPAAKSWQYPYGITFPGKPTGRFSDGRVLTDYLAKYLRVKSPIAYKW
Sbjct: 61  VFGDSYVDTGNNRKPAAKSWQYPYGITFPGKPTGRFSDGRVLTDYLAKYLRVKSPIAYKW 120

Query: 121 RKVGLGQLKYGMNFAYGGTGVFNTFIMSPNMSTQIDFLQQLIHESTYTAQDLHYSVALIS 180
           RKVGLGQLKYGMNFAYGGTGVFNTFIMSPNMSTQIDFLQQLIHESTYTAQDLHYSVALIS
Sbjct: 121 RKVGLGQLKYGMNFAYGGTGVFNTFIMSPNMSTQIDFLQQLIHESTYTAQDLHYSVALIS 180

Query: 181 LAGNDYGVYQATDGSAQSKYKKLSKEFGDFVLSLACWWQGWQPFITKVVNQLEVNLRRIH 240
           LAGNDYGVYQATDGSA                      QGWQPFITKVVNQLEVNLRRIH
Sbjct: 181 LAGNDYGVYQATDGSA----------------------QGWQPFITKVVNQLEVNLRRIH 240

Query: 241 GLGVPKIVVTSLEPLGCLPSSTVASSFQQCNATENLLVNFHNLLLQQSVAKLNSEVKDAS 300
           GLGVPKIVVTSLEPLGCLPSSTVASSFQQCNATENLLVNFHNLLLQQSVAKLNSEVKDAS
Sbjct: 241 GLGVPKIVVTSLEPLGCLPSSTVASSFQQCNATENLLVNFHNLLLQQSVAKLNSEVKDAS 300

Query: 301 ASTFILLDLYSSFMAALNNKEDPLGNVKFENPLKPCCVGISSEYACGNVGANGEKKYTIC 360
           ASTFILLDLYSSFMAALNNKEDPLGNVKFENPLKPCCVGISSEYACGNVGANGEKKYTIC
Sbjct: 301 ASTFILLDLYSSFMAALNNKEDPLGNVKFENPLKPCCVGISSEYACGNVGANGEKKYTIC 360

Query: 361 EDPGAAFFWDEVHPSQNGWFAVYSALQANLKQL 394
           EDPGAAFFWDEVHPSQNGWFAVYSALQANLKQL
Sbjct: 361 EDPGAAFFWDEVHPSQNGWFAVYSALQANLKQL 371

BLAST of Carg14526 vs. NCBI nr
Match: XP_023539454.1 (GDSL esterase/lipase At5g03610-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 748.4 bits (1931), Expect = 3.1e-212
Identity = 368/393 (93.64%), Postives = 369/393 (93.89%), Query Frame = 0

Query: 1   MDCKKLLCFSLLCFSLLPELYGVVWVSAGGGGHRHRHRVRRSGQSLGGGDLSDSNPRKLF 60
           MDCKKLLCFSLLCFSLLPELYGVVWVSAGGGGHRHRHRVRRSGQSLGGG+LSDSNPRKLF
Sbjct: 1   MDCKKLLCFSLLCFSLLPELYGVVWVSAGGGGHRHRHRVRRSGQSLGGGELSDSNPRKLF 60

Query: 61  VFGDSYVDTGNNRKPAAKSWQYPYGITFPGKPTGRFSDGRVLTDYLAKYLRVKSPIAYKW 120
           VFGDSYVDTGNNRKPAAKSWQYPYGITFPGKPTGRFSDGRVLTDYLAKYLRVKSPIAYKW
Sbjct: 61  VFGDSYVDTGNNRKPAAKSWQYPYGITFPGKPTGRFSDGRVLTDYLAKYLRVKSPIAYKW 120

Query: 121 RKVGLGQLKYGMNFAYGGTGVFNTFIMSPNMSTQIDFLQQLIHESTYTAQDLHYSVALIS 180
           RKVGLGQLKYGMNFAYGGTGVFNTFIMSPNMSTQIDFLQQLIHESTYT QDLHYSVALIS
Sbjct: 121 RKVGLGQLKYGMNFAYGGTGVFNTFIMSPNMSTQIDFLQQLIHESTYTTQDLHYSVALIS 180

Query: 181 LAGNDYGVYQATDGSAQSKYKKLSKEFGDFVLSLACWWQGWQPFITKVVNQLEVNLRRIH 240
           LAGNDYGVYQATDGSA                      QGWQPFITKVVNQLEVNLRRIH
Sbjct: 181 LAGNDYGVYQATDGSA----------------------QGWQPFITKVVNQLEVNLRRIH 240

Query: 241 GLGVPKIVVTSLEPLGCLPSSTVASSFQQCNATENLLVNFHNLLLQQSVAKLNSEVKDAS 300
           GLGVPKIVVTSLEPLGCLPSSTVASSFQQCNATENLLVNFHNLLLQQSVAKLNSEVKDAS
Sbjct: 241 GLGVPKIVVTSLEPLGCLPSSTVASSFQQCNATENLLVNFHNLLLQQSVAKLNSEVKDAS 300

Query: 301 ASTFILLDLYSSFMAALNNKEDPLGNVKFENPLKPCCVGISSEYACGNVGANGEKKYTIC 360
           ASTFILLDLYSSFMAALNNK DPLGNVKFENPLKPCCVGISSEYACGNVGANGEKKYTIC
Sbjct: 301 ASTFILLDLYSSFMAALNNKGDPLGNVKFENPLKPCCVGISSEYACGNVGANGEKKYTIC 360

Query: 361 EDPGAAFFWDEVHPSQNGWFAVYSALQANLKQL 394
           EDPGAAFFWDEVHPSQNGWFAVYSALQANLKQL
Sbjct: 361 EDPGAAFFWDEVHPSQNGWFAVYSALQANLKQL 371

BLAST of Carg14526 vs. NCBI nr
Match: XP_022926249.1 (GDSL esterase/lipase At5g03610-like [Cucurbita moschata])

HSP 1 Score: 746.5 bits (1926), Expect = 1.2e-211
Identity = 367/393 (93.38%), Postives = 368/393 (93.64%), Query Frame = 0

Query: 1   MDCKKLLCFSLLCFSLLPELYGVVWVSAGGGGHRHRHRVRRSGQSLGGGDLSDSNPRKLF 60
           MDCKKLLCFSLLCFSLLPELYGVVWVS GGGGHRHRHRVRRSGQSLGGG+LSDSNPRKLF
Sbjct: 1   MDCKKLLCFSLLCFSLLPELYGVVWVSVGGGGHRHRHRVRRSGQSLGGGELSDSNPRKLF 60

Query: 61  VFGDSYVDTGNNRKPAAKSWQYPYGITFPGKPTGRFSDGRVLTDYLAKYLRVKSPIAYKW 120
           VFGDSYVDTGNNRKPAAKSWQYPYGITFPGKPTGRFSDGRVLTDYLAKYLRVKSPIAYKW
Sbjct: 61  VFGDSYVDTGNNRKPAAKSWQYPYGITFPGKPTGRFSDGRVLTDYLAKYLRVKSPIAYKW 120

Query: 121 RKVGLGQLKYGMNFAYGGTGVFNTFIMSPNMSTQIDFLQQLIHESTYTAQDLHYSVALIS 180
           RKVGLGQLKYGMNFAYGGTGVFNTFIMSPNMSTQIDFLQQLIHESTYTAQDLHYSVALIS
Sbjct: 121 RKVGLGQLKYGMNFAYGGTGVFNTFIMSPNMSTQIDFLQQLIHESTYTAQDLHYSVALIS 180

Query: 181 LAGNDYGVYQATDGSAQSKYKKLSKEFGDFVLSLACWWQGWQPFITKVVNQLEVNLRRIH 240
           LAGNDYGVYQATDGSA                      QGWQPFITKVVNQLEVNLRRIH
Sbjct: 181 LAGNDYGVYQATDGSA----------------------QGWQPFITKVVNQLEVNLRRIH 240

Query: 241 GLGVPKIVVTSLEPLGCLPSSTVASSFQQCNATENLLVNFHNLLLQQSVAKLNSEVKDAS 300
           GLGVPKIVVTSLEPLGCLPSSTVASSF QCNATENLLVNFHNLLLQQSVAKLNSEVKDAS
Sbjct: 241 GLGVPKIVVTSLEPLGCLPSSTVASSFHQCNATENLLVNFHNLLLQQSVAKLNSEVKDAS 300

Query: 301 ASTFILLDLYSSFMAALNNKEDPLGNVKFENPLKPCCVGISSEYACGNVGANGEKKYTIC 360
           ASTFILLDLYSSFMAALNNK DPLGNVKFENPLKPCCVGISSEYACGNVGANGEKKYTIC
Sbjct: 301 ASTFILLDLYSSFMAALNNKGDPLGNVKFENPLKPCCVGISSEYACGNVGANGEKKYTIC 360

Query: 361 EDPGAAFFWDEVHPSQNGWFAVYSALQANLKQL 394
           EDPGAAFFWDEVHPSQNGWFAVYSALQANLKQL
Sbjct: 361 EDPGAAFFWDEVHPSQNGWFAVYSALQANLKQL 371

BLAST of Carg14526 vs. NCBI nr
Match: XP_023005883.1 (GDSL esterase/lipase At5g03610-like [Cucurbita maxima])

HSP 1 Score: 738.8 bits (1906), Expect = 2.4e-209
Identity = 365/393 (92.88%), Postives = 367/393 (93.38%), Query Frame = 0

Query: 1   MDCKKLLCFSLLCFSLLPELYGVVWVSAGGGGHRHRHRVRRSGQSLGGGDLSDSNPRKLF 60
           MD KKLLCFSLLCFSLLPELYGVVWVSAGG GHRHRHRVRRSGQ LGGG+LSDSNPRKLF
Sbjct: 1   MDSKKLLCFSLLCFSLLPELYGVVWVSAGGVGHRHRHRVRRSGQRLGGGELSDSNPRKLF 60

Query: 61  VFGDSYVDTGNNRKPAAKSWQYPYGITFPGKPTGRFSDGRVLTDYLAKYLRVKSPIAYKW 120
           VFGDSYVDTGNNRKPAAKSWQYPYGITFPGKPTGRFSDGRVLTDYLAKYLRVKSPIAYKW
Sbjct: 61  VFGDSYVDTGNNRKPAAKSWQYPYGITFPGKPTGRFSDGRVLTDYLAKYLRVKSPIAYKW 120

Query: 121 RKVGLGQLKYGMNFAYGGTGVFNTFIMSPNMSTQIDFLQQLIHESTYTAQDLHYSVALIS 180
           RKVGLGQLKYGMNFAYGGTGVFNTFIMSPNMSTQIDFLQQLIHESTYTAQDLHYSVALIS
Sbjct: 121 RKVGLGQLKYGMNFAYGGTGVFNTFIMSPNMSTQIDFLQQLIHESTYTAQDLHYSVALIS 180

Query: 181 LAGNDYGVYQATDGSAQSKYKKLSKEFGDFVLSLACWWQGWQPFITKVVNQLEVNLRRIH 240
           LAGNDYGVYQATDGSA                      QGWQPFITKVVNQLEVNLRRIH
Sbjct: 181 LAGNDYGVYQATDGSA----------------------QGWQPFITKVVNQLEVNLRRIH 240

Query: 241 GLGVPKIVVTSLEPLGCLPSSTVASSFQQCNATENLLVNFHNLLLQQSVAKLNSEVKDAS 300
           GLGVPKIVVTSLEPLGCLPSSTVASSFQQCNATENLLVNFHNLLLQQSVAKLNSEVKDAS
Sbjct: 241 GLGVPKIVVTSLEPLGCLPSSTVASSFQQCNATENLLVNFHNLLLQQSVAKLNSEVKDAS 300

Query: 301 ASTFILLDLYSSFMAALNNKEDPLGNVKFENPLKPCCVGISSEYACGNVGANGEKKYTIC 360
           ASTFILLDLYSSFMAALNNK DPLGNVKFENPLKPCCVGISSEYACGNVGANGEKKYTIC
Sbjct: 301 ASTFILLDLYSSFMAALNNKGDPLGNVKFENPLKPCCVGISSEYACGNVGANGEKKYTIC 360

Query: 361 EDPGAAFFWDEVHPSQNGWFAVYSALQANLKQL 394
           E+PGAAFFWDEVHPSQNGWFAVYSALQANLKQL
Sbjct: 361 ENPGAAFFWDEVHPSQNGWFAVYSALQANLKQL 371

BLAST of Carg14526 vs. ExPASy Swiss-Prot
Match: Q9LZS7 (GDSL esterase/lipase At5g03610 OS=Arabidopsis thaliana OX=3702 GN=At5g03610 PE=2 SV=1)

HSP 1 Score: 368.6 bits (945), Expect = 8.7e-101
Identity = 196/392 (50.00%), Postives = 246/392 (62.76%), Query Frame = 0

Query: 1   MDCKKLLCFSLLCFSLLPELYGVVWVSAGGGGHRHRHRVRRSGQSLGGGDLSDSNPRKLF 60
           MD    L F L  F     L+G +    G   + H +  R               P KLF
Sbjct: 1   MDSLIKLFFCLFIFLCTSLLFGEINGVEGSNQNHHLYPFR---------------PTKLF 60

Query: 61  VFGDSYVDTGNNRKPAAKSWQYPYGITFPGKPTGRFSDGRVLTDYLAKYLRVKSPIAYKW 120
           VFGDSY DTGN +K  + SW++PYGITFPGKP GRFSDGRV TD+LAK++ +KSPI Y W
Sbjct: 61  VFGDSYADTGNIKKAFSSSWKFPYGITFPGKPAGRFSDGRVATDFLAKFVGIKSPIPYFW 120

Query: 121 RK-VGLGQLKYGMNFAYGGTGVFNTFIMSPNMSTQIDFLQQLIHE-STYTAQDLHYSVAL 180
           +   G  +L+YGMNFAYGGTGVFNT    PNM+TQID  Q ++     Y   +L  SVAL
Sbjct: 121 KDYAGKKRLQYGMNFAYGGTGVFNTQTPLPNMTTQIDIFQNILTTGDIYYPPELTSSVAL 180

Query: 181 ISLAGNDYGVYQATDGSAQSKYKKLSKEFGDFVLSLACWWQGWQPFITKVVNQLEVNLRR 240
           +S+AGNDY           S +  L++   +F             FI +VV+Q EVNLRR
Sbjct: 181 VSVAGNDY-----------SNFIALNRPASEF-----------PAFIKQVVDQTEVNLRR 240

Query: 241 IHGLGVPKIVVTSLEPLGCLPSSTVASSFQQCNATENLLVNFHNLLLQQSVAKLNSEVKD 300
           IH LGV KI V SL+PLGCLP  T  +SFQ+CN T+N LVN HN LLQQ VAKLN+E K 
Sbjct: 241 IHALGVKKIAVPSLQPLGCLPPFTFVTSFQRCNETQNALVNLHNNLLQQVVAKLNNETKQ 300

Query: 301 ASASTFILLDLYSSFMAALNNKEDPLGNVKFENPLKPCCVGISSEYACGNVGANGEKKYT 360
              STFI+LDLY++F+    NK    G+ +FE+PLKPCCVG+S EY CG+V   G KKY 
Sbjct: 301 ---STFIILDLYNAFLTVFKNKGSNPGSTRFESPLKPCCVGVSREYNCGSVDEKGVKKYI 352

Query: 361 ICEDPGAAFFWDEVHPSQNGWFAVYSALQANL 391
           +C++P  AFFWD +HP++ GW +VYS L+ +L
Sbjct: 361 VCDNPKTAFFWDGLHPTEEGWRSVYSVLRESL 352

BLAST of Carg14526 vs. ExPASy Swiss-Prot
Match: Q9SF94 (GDSL esterase/lipase At3g09930 OS=Arabidopsis thaliana OX=3702 GN=At3g09930 PE=2 SV=1)

HSP 1 Score: 351.3 bits (900), Expect = 1.4e-95
Identity = 179/347 (51.59%), Postives = 230/347 (66.28%), Query Frame = 0

Query: 50  DLSDSNPRKLFVFGDSYVDTGNNRKPAAKSWQYPYGITFPGKPTGRFSDGRVLTDYLAKY 109
           +L    P +LFVFGDSY DTGN RK  + SW+ PYGITFP KP+GRFSDGRV TD+LA+Y
Sbjct: 31  NLKRLRPNRLFVFGDSYADTGNIRKSLSDSWKIPYGITFPQKPSGRFSDGRVATDFLARY 90

Query: 110 LRVKSPIAYKWRK-VGLGQLKYGMNFAYGGTGVFNTFIMS-PNMSTQIDFLQQLIHE-ST 169
           L +KSPI Y W+   G  +L YGMN+AYGGTGVF T     PNM+TQID+ Q+++   + 
Sbjct: 91  LGIKSPIPYTWKDYAGKERLLYGMNYAYGGTGVFKTKDNPLPNMTTQIDYFQRVLAAGNI 150

Query: 170 YTAQDLHYSVALISLAGNDYGVYQATDGSAQSKYKKLSKEFGDFVLSLACWWQGWQPFIT 229
           Y+  DL  S+AL+S+AGNDY  + A           L +   +              F+ 
Sbjct: 151 YSPSDLPSSLALVSVAGNDYATFLA-----------LKRPLTEL-----------PAFMK 210

Query: 230 KVVNQLEVNLRRIHGLGVPKIVVTSLEPLGCLPSSTVASSFQQCNATENLLVNFHNLLLQ 289
           +VV+Q+ VN  RIH LGV KIV+ S++PLGCLPS TV +SFQ+CNAT+N   N HN LL 
Sbjct: 211 QVVDQIAVNAMRIHKLGVNKIVIPSMQPLGCLPSITVFNSFQRCNATDNASTNLHNYLLH 270

Query: 290 QSVAKLNSEVKDASASTFILLDLYSSFMAALNNKEDPLGNVKFENPLKPCCVGISSEYAC 349
           +++A+LN+E K    STF++LD Y++F+    NK    G  +F NPLKPCCVG++S Y C
Sbjct: 271 KAIARLNNETK---PSTFVVLDHYNAFLTVFKNKGPEPGVSRFGNPLKPCCVGVNSSYDC 330

Query: 350 GNVGANGEKKYTICEDPGAAFFWDEVHPSQNGWFAVYSALQANLKQL 394
            NV   GEKKY ICEDP AAFFWD  HPS+ GW +VYS L  +LK +
Sbjct: 331 SNVDEKGEKKYIICEDPKAAFFWDIFHPSEEGWRSVYSVLHKHLKAI 352

BLAST of Carg14526 vs. ExPASy Swiss-Prot
Match: Q9LZS9 (GDSL esterase/lipase At5g03590 OS=Arabidopsis thaliana OX=3702 GN=At5g03590 PE=3 SV=2)

HSP 1 Score: 263.5 bits (672), Expect = 3.9e-69
Identity = 154/347 (44.38%), Postives = 206/347 (59.37%), Query Frame = 0

Query: 52  SDSNPRKLFVFGDSYVDTGNNRKPAAKSWQYPYGITFPGKPTGRFSDGRVLTDYLAKYLR 111
           ++ NP KLFVFG+SY DTG N KP A SW+ PYGITFPGKP+GR+SDG   TD+LAK L 
Sbjct: 29  ANKNP-KLFVFGNSYADTG-NMKPTALSWKLPYGITFPGKPSGRYSDGLTATDFLAKQLG 88

Query: 112 VKSPIAYKWRKVGLGQLKY--GMNFAYGGTGVFNTFI-MSPNMSTQIDFLQQL-IHESTY 171
            K P  Y WR  G  ++K   GMNFA+GG+ VF++ +  SPN+STQ+ FL  L +    Y
Sbjct: 89  AKLP--YLWRTHGKKKVKLNRGMNFAFGGSEVFDSPVDRSPNISTQVGFLVNLALARRVY 148

Query: 172 TAQ-DLHYSVALISLAGNDYGVYQATDGSAQSKYKKLSKEFGDFVLSLACWWQGWQPFIT 231
           T   DL  S AL+S +G DY  Y   D +                         +  F+ 
Sbjct: 149 TIDGDLASSYALLSYSGTDY--YGFIDQNPN--------------------MAAYPAFVE 208

Query: 232 KVVNQLEVNLRRIHGLGVPKIVVTSLEPLGCLPSSTVASSFQQCNATENLLVNFHNLLLQ 291
            +V  ++ +L  ++GL    I VTSL PLGCLP  TVASSF+ CN + + LV  HN  L+
Sbjct: 209 FIVEDIQYSLGIMNGLKFKNIAVTSLHPLGCLPRVTVASSFRSCNESYSDLVRLHNESLK 268

Query: 292 QSVAKLNSEVK-DASASTFILLDLYSSFMAALNNKEDPLGNVKFENPLKPCCVGISSEYA 351
           ++VAKLN E K       F+++DL+ +FM  L  K    GN +F++PLKPCC G      
Sbjct: 269 KAVAKLNKEDKFRTKGDRFVIVDLHKAFMTILEKK----GNKRFKSPLKPCCEG-----D 328

Query: 352 CGNVGANGEKKYTICEDPGAAFFWDEVHPSQNGWFAVYSALQANLKQ 393
           C  +   G KKYT+C DP +AFFWDE++P+Q GW ++YS L  +L +
Sbjct: 329 CARMDMKGAKKYTLCNDPKSAFFWDEINPTQEGWRSIYSLLGKSLTE 340

BLAST of Carg14526 vs. ExPASy Swiss-Prot
Match: Q8RWJ4 (GDSL esterase/lipase At2g36325 OS=Arabidopsis thaliana OX=3702 GN=At2g36325 PE=2 SV=2)

HSP 1 Score: 256.1 bits (653), Expect = 6.3e-67
Identity = 143/334 (42.81%), Postives = 188/334 (56.29%), Query Frame = 0

Query: 56  PRKLFVFGDSYVDTGNNRKPAAKSWQYPYGITFPGKPTGRFSDGRVLTDYLAKYLRVKSP 115
           P KLFVFGDSY DTGN       SW++P GITFPG PTGRFSDGRV TDYLAKY+ V++P
Sbjct: 45  PTKLFVFGDSYADTGNTPFLIVPSWRFPNGITFPGIPTGRFSDGRVSTDYLAKYIGVRTP 104

Query: 116 IAYKWRKVGLGQL--KYGMNFAYGGTGVFNT-FIMSPNMSTQIDFLQQLIHESTYTAQDL 175
           I YKW K G  +L  K GMNFAYGG G F T F + P  S QID  +QL+  + Y+  DL
Sbjct: 105 ITYKWGKYGRPRLAVKRGMNFAYGGAGAFETMFKLVPTASVQIDSFEQLLMRNVYSPADL 164

Query: 176 HYSVALISLAGNDYGVYQATDGSAQSKYKKLSKEFGDFVLSLACWWQGWQPFITKVVNQL 235
           + SVA  S+ GNDY  Y   +GS                       +G      KVV Q+
Sbjct: 165 NSSVAFFSIIGNDYLTYDRRNGSE----------------------EGRSALTRKVVKQI 224

Query: 236 EVNLRRIHGLGVPKIVVTSLEPLGCLPSSTVASSFQQCNATENLLVNFHNLLLQQSVAKL 295
            ++++RI  LGV K++V    P  CLP          C+ T +     HN LL++ + KL
Sbjct: 225 LLDVKRIKDLGVRKVLVALSPPQKCLPKLVTPKG---CD-TNDTSTYLHNSLLRKGLIKL 284

Query: 296 NSEVKDASASTFILLDLYSSFMAALNNKEDPLGNVKFENPLKPCCVGISSEYACGNVGAN 355
           N +  + +  +F+ LDLY++F+    NK    G   F +P K CC      + CG+   +
Sbjct: 285 NDKEINNNDKSFMTLDLYNAFVTIFKNK-GVSGVSTFPDPFKACCATKRGTF-CGDRSLS 344

Query: 356 GEKKYTICEDPGAAFFWDEVHPSQNGWFAVYSAL 387
           G+K YT+C+DP + FFWD VH S  GW +V+S L
Sbjct: 345 GKKLYTLCDDPKSFFFWDNVHISDQGWRSVFSLL 350

BLAST of Carg14526 vs. ExPASy Swiss-Prot
Match: Q9LZS8 (GDSL esterase/lipase At5g03600 OS=Arabidopsis thaliana OX=3702 GN=At5g03600 PE=3 SV=1)

HSP 1 Score: 247.3 bits (630), Expect = 2.9e-64
Identity = 138/332 (41.57%), Postives = 194/332 (58.43%), Query Frame = 0

Query: 58  KLFVFGDSYVDTGNNRKPAAKSWQYPYGITFPGKPTGRFSDGRVLTDYLAKYLRVKSPIA 117
           KLFVFGDSY DTGN ++   ++W  PYGITFPGKP+GR+ DG + TD+L K L  +SP  
Sbjct: 14  KLFVFGDSYADTGNTKRD-TEAWAIPYGITFPGKPSGRYCDGLIATDFLEKVLGAESPYL 73

Query: 118 YKWRKVGLGQLKYGMNFAYGGTGVFNTFIMS--PNMSTQIDFLQQLIHESTYTAQDLHYS 177
           Y+      G LK GMNFA+GG+ + ++   S  PN++ Q++FL  L+             
Sbjct: 74  YRTHGRDKG-LKRGMNFAFGGSKMLDSSPNSPFPNITAQVNFLVDLVLAGRVYGDITPSD 133

Query: 178 VALISLAGNDYGVYQATDGSAQSKYKKLSKEFGDFVLSLACWWQGWQPFITKVVNQLEVN 237
           V+LIS AG DY  Y   +  A                       G +  + KVV+ L VN
Sbjct: 134 VSLISYAGGDYIYYIDQNRPA----------------------AGLKALVEKVVDNLRVN 193

Query: 238 LRRIHGLGVPKIVVTSLEPLGCLPSSTVASSFQQCNATENLLVNFHNLLLQQSVAKLNSE 297
           +  + GL   KI VTSL+P+GCLPS T ASSF+ CN +++ LV  HN LL++ VAKLN +
Sbjct: 194 MIVLGGLLFKKIAVTSLQPIGCLPSYTSASSFKSCNESQSALVELHNKLLKKVVAKLNEQ 253

Query: 298 VKDASAST-FILLDLYSSFMAALNNKEDPLGNVKFENPLKPCCVGISSEYACGNVGANGE 357
            +       F ++D++++FM  + NK    G+ +F+NP+K CC G      CG   ++G 
Sbjct: 254 SRVMKKEQHFFIIDIHNAFMTVMKNK----GSKRFKNPMKSCCEGY-----CGR-SSDGG 311

Query: 358 KKYTICEDPGAAFFWDEVHPSQNGWFAVYSAL 387
           K YT+C+DP + FFWD VHP+Q GW ++YS L
Sbjct: 314 KLYTLCDDPKSFFFWDAVHPTQEGWRSIYSVL 311

BLAST of Carg14526 vs. ExPASy TrEMBL
Match: A0A6J1EEL4 (GDSL esterase/lipase At5g03610-like OS=Cucurbita moschata OX=3662 GN=LOC111433403 PE=3 SV=1)

HSP 1 Score: 746.5 bits (1926), Expect = 5.6e-212
Identity = 367/393 (93.38%), Postives = 368/393 (93.64%), Query Frame = 0

Query: 1   MDCKKLLCFSLLCFSLLPELYGVVWVSAGGGGHRHRHRVRRSGQSLGGGDLSDSNPRKLF 60
           MDCKKLLCFSLLCFSLLPELYGVVWVS GGGGHRHRHRVRRSGQSLGGG+LSDSNPRKLF
Sbjct: 1   MDCKKLLCFSLLCFSLLPELYGVVWVSVGGGGHRHRHRVRRSGQSLGGGELSDSNPRKLF 60

Query: 61  VFGDSYVDTGNNRKPAAKSWQYPYGITFPGKPTGRFSDGRVLTDYLAKYLRVKSPIAYKW 120
           VFGDSYVDTGNNRKPAAKSWQYPYGITFPGKPTGRFSDGRVLTDYLAKYLRVKSPIAYKW
Sbjct: 61  VFGDSYVDTGNNRKPAAKSWQYPYGITFPGKPTGRFSDGRVLTDYLAKYLRVKSPIAYKW 120

Query: 121 RKVGLGQLKYGMNFAYGGTGVFNTFIMSPNMSTQIDFLQQLIHESTYTAQDLHYSVALIS 180
           RKVGLGQLKYGMNFAYGGTGVFNTFIMSPNMSTQIDFLQQLIHESTYTAQDLHYSVALIS
Sbjct: 121 RKVGLGQLKYGMNFAYGGTGVFNTFIMSPNMSTQIDFLQQLIHESTYTAQDLHYSVALIS 180

Query: 181 LAGNDYGVYQATDGSAQSKYKKLSKEFGDFVLSLACWWQGWQPFITKVVNQLEVNLRRIH 240
           LAGNDYGVYQATDGSA                      QGWQPFITKVVNQLEVNLRRIH
Sbjct: 181 LAGNDYGVYQATDGSA----------------------QGWQPFITKVVNQLEVNLRRIH 240

Query: 241 GLGVPKIVVTSLEPLGCLPSSTVASSFQQCNATENLLVNFHNLLLQQSVAKLNSEVKDAS 300
           GLGVPKIVVTSLEPLGCLPSSTVASSF QCNATENLLVNFHNLLLQQSVAKLNSEVKDAS
Sbjct: 241 GLGVPKIVVTSLEPLGCLPSSTVASSFHQCNATENLLVNFHNLLLQQSVAKLNSEVKDAS 300

Query: 301 ASTFILLDLYSSFMAALNNKEDPLGNVKFENPLKPCCVGISSEYACGNVGANGEKKYTIC 360
           ASTFILLDLYSSFMAALNNK DPLGNVKFENPLKPCCVGISSEYACGNVGANGEKKYTIC
Sbjct: 301 ASTFILLDLYSSFMAALNNKGDPLGNVKFENPLKPCCVGISSEYACGNVGANGEKKYTIC 360

Query: 361 EDPGAAFFWDEVHPSQNGWFAVYSALQANLKQL 394
           EDPGAAFFWDEVHPSQNGWFAVYSALQANLKQL
Sbjct: 361 EDPGAAFFWDEVHPSQNGWFAVYSALQANLKQL 371

BLAST of Carg14526 vs. ExPASy TrEMBL
Match: A0A6J1L3E4 (GDSL esterase/lipase At5g03610-like OS=Cucurbita maxima OX=3661 GN=LOC111498754 PE=3 SV=1)

HSP 1 Score: 738.8 bits (1906), Expect = 1.2e-209
Identity = 365/393 (92.88%), Postives = 367/393 (93.38%), Query Frame = 0

Query: 1   MDCKKLLCFSLLCFSLLPELYGVVWVSAGGGGHRHRHRVRRSGQSLGGGDLSDSNPRKLF 60
           MD KKLLCFSLLCFSLLPELYGVVWVSAGG GHRHRHRVRRSGQ LGGG+LSDSNPRKLF
Sbjct: 1   MDSKKLLCFSLLCFSLLPELYGVVWVSAGGVGHRHRHRVRRSGQRLGGGELSDSNPRKLF 60

Query: 61  VFGDSYVDTGNNRKPAAKSWQYPYGITFPGKPTGRFSDGRVLTDYLAKYLRVKSPIAYKW 120
           VFGDSYVDTGNNRKPAAKSWQYPYGITFPGKPTGRFSDGRVLTDYLAKYLRVKSPIAYKW
Sbjct: 61  VFGDSYVDTGNNRKPAAKSWQYPYGITFPGKPTGRFSDGRVLTDYLAKYLRVKSPIAYKW 120

Query: 121 RKVGLGQLKYGMNFAYGGTGVFNTFIMSPNMSTQIDFLQQLIHESTYTAQDLHYSVALIS 180
           RKVGLGQLKYGMNFAYGGTGVFNTFIMSPNMSTQIDFLQQLIHESTYTAQDLHYSVALIS
Sbjct: 121 RKVGLGQLKYGMNFAYGGTGVFNTFIMSPNMSTQIDFLQQLIHESTYTAQDLHYSVALIS 180

Query: 181 LAGNDYGVYQATDGSAQSKYKKLSKEFGDFVLSLACWWQGWQPFITKVVNQLEVNLRRIH 240
           LAGNDYGVYQATDGSA                      QGWQPFITKVVNQLEVNLRRIH
Sbjct: 181 LAGNDYGVYQATDGSA----------------------QGWQPFITKVVNQLEVNLRRIH 240

Query: 241 GLGVPKIVVTSLEPLGCLPSSTVASSFQQCNATENLLVNFHNLLLQQSVAKLNSEVKDAS 300
           GLGVPKIVVTSLEPLGCLPSSTVASSFQQCNATENLLVNFHNLLLQQSVAKLNSEVKDAS
Sbjct: 241 GLGVPKIVVTSLEPLGCLPSSTVASSFQQCNATENLLVNFHNLLLQQSVAKLNSEVKDAS 300

Query: 301 ASTFILLDLYSSFMAALNNKEDPLGNVKFENPLKPCCVGISSEYACGNVGANGEKKYTIC 360
           ASTFILLDLYSSFMAALNNK DPLGNVKFENPLKPCCVGISSEYACGNVGANGEKKYTIC
Sbjct: 301 ASTFILLDLYSSFMAALNNKGDPLGNVKFENPLKPCCVGISSEYACGNVGANGEKKYTIC 360

Query: 361 EDPGAAFFWDEVHPSQNGWFAVYSALQANLKQL 394
           E+PGAAFFWDEVHPSQNGWFAVYSALQANLKQL
Sbjct: 361 ENPGAAFFWDEVHPSQNGWFAVYSALQANLKQL 371

BLAST of Carg14526 vs. ExPASy TrEMBL
Match: A0A6J1J6I3 (GDSL esterase/lipase At5g03610-like OS=Cucurbita maxima OX=3661 GN=LOC111481670 PE=3 SV=1)

HSP 1 Score: 675.2 bits (1741), Expect = 1.6e-190
Identity = 331/392 (84.44%), Postives = 350/392 (89.29%), Query Frame = 0

Query: 1   MDCKKLLCFSLLCFSLLPELYGVVWVSAGGGGHRHRHRVRRSGQSLGGGDLSDSNPRKLF 60
           MD K LLCFS+LCFSLLPEL GVV VSAG G HRHRHR RRS QSLGGG+L +SNPRKLF
Sbjct: 1   MDSKSLLCFSILCFSLLPELSGVVRVSAGRGSHRHRHRERRSSQSLGGGELWNSNPRKLF 60

Query: 61  VFGDSYVDTGNNRKPAAKSWQYPYGITFPGKPTGRFSDGRVLTDYLAKYLRVKSPIAYKW 120
           VFGDSYVDTGNNRKPAAKSWQYPYG+TFPGKPTGRFSDGRVLTDYLAKYL+VKSPI YKW
Sbjct: 61  VFGDSYVDTGNNRKPAAKSWQYPYGVTFPGKPTGRFSDGRVLTDYLAKYLKVKSPIPYKW 120

Query: 121 RKVGLGQLKYGMNFAYGGTGVFNTFIMSPNMSTQIDFLQQLIHESTYTAQDLHYSVALIS 180
           RKVG GQLKYGMNFAYGGTGVFNTF+MSPNMSTQIDF QQLI E T+T+QDL  SVAL+S
Sbjct: 121 RKVGYGQLKYGMNFAYGGTGVFNTFVMSPNMSTQIDFFQQLIGELTFTSQDLQSSVALVS 180

Query: 181 LAGNDYGVYQATDGSAQSKYKKLSKEFGDFVLSLACWWQGWQPFITKVVNQLEVNLRRIH 240
           LAGNDY VYQATDGSA                      QGWQPFITKVVNQLEVNLRRIH
Sbjct: 181 LAGNDYSVYQATDGSA----------------------QGWQPFITKVVNQLEVNLRRIH 240

Query: 241 GLGVPKIVVTSLEPLGCLPSSTVASSFQQCNATENLLVNFHNLLLQQSVAKLNSEVKDAS 300
           G+GVPKIVVT+LEPLGCLPSSTVASSFQQCNATEN LVNFHN+LLQQSVAKLNSEVKDAS
Sbjct: 241 GVGVPKIVVTALEPLGCLPSSTVASSFQQCNATENQLVNFHNMLLQQSVAKLNSEVKDAS 300

Query: 301 ASTFILLDLYSSFMAALNNKEDPLGNVKFENPLKPCCVGISSEYACGNVGANGEKKYTIC 360
           ASTFILLDLYSSFMAALNNK D LGNVKFENPLKPCCVG+SSEY+CG++G+NGEKKYTIC
Sbjct: 301 ASTFILLDLYSSFMAALNNKGDHLGNVKFENPLKPCCVGVSSEYSCGSMGSNGEKKYTIC 360

Query: 361 EDPGAAFFWDEVHPSQNGWFAVYSALQANLKQ 393
           EDPGAAFFWDEVHP+Q+GWFAVYSALQANLKQ
Sbjct: 361 EDPGAAFFWDEVHPTQHGWFAVYSALQANLKQ 370

BLAST of Carg14526 vs. ExPASy TrEMBL
Match: A0A6J1FAT3 (GDSL esterase/lipase At5g03610-like OS=Cucurbita moschata OX=3662 GN=LOC111442352 PE=3 SV=1)

HSP 1 Score: 672.5 bits (1734), Expect = 1.0e-189
Identity = 330/392 (84.18%), Postives = 350/392 (89.29%), Query Frame = 0

Query: 1   MDCKKLLCFSLLCFSLLPELYGVVWVSAGGGGHRHRHRVRRSGQSLGGGDLSDSNPRKLF 60
           MD K LLCFS+LCFSLLPEL GVV VSAG   HRHRHR RRS QSLGGG+L +SNPRKLF
Sbjct: 1   MDSKTLLCFSILCFSLLPELSGVVRVSAGRVSHRHRHRERRSSQSLGGGELWNSNPRKLF 60

Query: 61  VFGDSYVDTGNNRKPAAKSWQYPYGITFPGKPTGRFSDGRVLTDYLAKYLRVKSPIAYKW 120
           VFGDSYVDTGNNRKPAAKSWQYPYG+TFPGKPTGRFSDGRVLTDYLAKYL+VKSPI YKW
Sbjct: 61  VFGDSYVDTGNNRKPAAKSWQYPYGVTFPGKPTGRFSDGRVLTDYLAKYLKVKSPIPYKW 120

Query: 121 RKVGLGQLKYGMNFAYGGTGVFNTFIMSPNMSTQIDFLQQLIHESTYTAQDLHYSVALIS 180
           RKVG GQLKYGMNFAYGGTGVFNTF+MSPNMSTQIDF QQLI EST+T+QDL  SVAL+S
Sbjct: 121 RKVGYGQLKYGMNFAYGGTGVFNTFVMSPNMSTQIDFFQQLIGESTFTSQDLQSSVALVS 180

Query: 181 LAGNDYGVYQATDGSAQSKYKKLSKEFGDFVLSLACWWQGWQPFITKVVNQLEVNLRRIH 240
           LAGNDY VYQATDGSA                      QGWQPFITKVVNQLEVNLRRIH
Sbjct: 181 LAGNDYSVYQATDGSA----------------------QGWQPFITKVVNQLEVNLRRIH 240

Query: 241 GLGVPKIVVTSLEPLGCLPSSTVASSFQQCNATENLLVNFHNLLLQQSVAKLNSEVKDAS 300
           G+GVPKIVVT+LEPLGCLPSSTVASSFQQCNATEN LVNFHN+LLQQSVAKLNSEVKDAS
Sbjct: 241 GVGVPKIVVTALEPLGCLPSSTVASSFQQCNATENQLVNFHNMLLQQSVAKLNSEVKDAS 300

Query: 301 ASTFILLDLYSSFMAALNNKEDPLGNVKFENPLKPCCVGISSEYACGNVGANGEKKYTIC 360
           ASTFILLDLYSSFMAALNNK D LGNVKFENPLKPCCVG+SSEY+CG++G+NGEKKYTIC
Sbjct: 301 ASTFILLDLYSSFMAALNNKGDHLGNVKFENPLKPCCVGVSSEYSCGSMGSNGEKKYTIC 360

Query: 361 EDPGAAFFWDEVHPSQNGWFAVYSALQANLKQ 393
           +DPGAAFFWDEVHP+Q+GWFAVYSALQANLKQ
Sbjct: 361 DDPGAAFFWDEVHPTQHGWFAVYSALQANLKQ 370

BLAST of Carg14526 vs. ExPASy TrEMBL
Match: A0A1S3B7C0 (GDSL esterase/lipase At5g03610 OS=Cucumis melo OX=3656 GN=LOC103486596 PE=3 SV=1)

HSP 1 Score: 657.9 bits (1696), Expect = 2.6e-185
Identity = 328/395 (83.04%), Postives = 346/395 (87.59%), Query Frame = 0

Query: 1   MDCKKLLCFSLLCFSLLPELYGVVWVSAGGGGHRHRHRVRRSGQSLGGGDLSDSNPRKLF 60
           MD KKLL   LLCFSLLPELYGVVWVSAGGG HR + R     QSLGGG+LSDSNPRK+F
Sbjct: 1   MDSKKLLSLYLLCFSLLPELYGVVWVSAGGGAHREQRR----SQSLGGGELSDSNPRKMF 60

Query: 61  VFGDSYVDTGNNRKPAAKSWQYPYGITFPGKPTGRFSDGRVLTDYLAKYLRVKSPIAYKW 120
           VFGDSYVDTGNNRKPAAKSWQYPYGITFPGKPTGRFSDGRVLTDYLAKYL+VKSPI YKW
Sbjct: 61  VFGDSYVDTGNNRKPAAKSWQYPYGITFPGKPTGRFSDGRVLTDYLAKYLKVKSPIPYKW 120

Query: 121 RKV--GLGQLKYGMNFAYGGTGVFNTFIMSPNMSTQIDFLQQLIHESTYTAQDLHYSVAL 180
           RKV  GLGQLKYGMNFAYGGTGVFNTF+ SPNMSTQIDFLQQL+ EST+T +DL  SVAL
Sbjct: 121 RKVGFGLGQLKYGMNFAYGGTGVFNTFVTSPNMSTQIDFLQQLVGESTFTGRDLLSSVAL 180

Query: 181 ISLAGNDYGVYQATDGSAQSKYKKLSKEFGDFVLSLACWWQGWQPFITKVVNQLEVNLRR 240
           +SLAGNDY VYQAT+GS                       QGWQPFITKVVNQLEVNLRR
Sbjct: 181 VSLAGNDYSVYQATNGSP----------------------QGWQPFITKVVNQLEVNLRR 240

Query: 241 IHGLGVPKIVVTSLEPLGCLPSSTVASSFQQCNATENLLVNFHNLLLQQSVAKLNSEVKD 300
           IHGLGVPKIVVT+LEPLGCLPS+TVASSFQQCN+T+N LVNFHNLLLQQSVAKLNSEVKD
Sbjct: 241 IHGLGVPKIVVTALEPLGCLPSTTVASSFQQCNSTQNQLVNFHNLLLQQSVAKLNSEVKD 300

Query: 301 ASASTFILLDLYSSFMAALNNKEDPLGNVKFENPLKPCCVGISSEYACGNVGANGEKKYT 360
            SASTFILLDLYSSFMAALNNK D LGNVKFENP+KPCCVGISSEYACG+VGANGEKKYT
Sbjct: 301 PSASTFILLDLYSSFMAALNNKVDQLGNVKFENPMKPCCVGISSEYACGSVGANGEKKYT 360

Query: 361 ICEDPGAAFFWDEVHPSQNGWFAVYSALQANLKQL 394
           ICEDPGAAFFWDEVHP+Q GW+AVYSALQANLKQL
Sbjct: 361 ICEDPGAAFFWDEVHPTQYGWYAVYSALQANLKQL 369

BLAST of Carg14526 vs. TAIR 10
Match: AT5G03610.1 (GDSL-like Lipase/Acylhydrolase superfamily protein )

HSP 1 Score: 368.6 bits (945), Expect = 6.2e-102
Identity = 196/392 (50.00%), Postives = 246/392 (62.76%), Query Frame = 0

Query: 1   MDCKKLLCFSLLCFSLLPELYGVVWVSAGGGGHRHRHRVRRSGQSLGGGDLSDSNPRKLF 60
           MD    L F L  F     L+G +    G   + H +  R               P KLF
Sbjct: 1   MDSLIKLFFCLFIFLCTSLLFGEINGVEGSNQNHHLYPFR---------------PTKLF 60

Query: 61  VFGDSYVDTGNNRKPAAKSWQYPYGITFPGKPTGRFSDGRVLTDYLAKYLRVKSPIAYKW 120
           VFGDSY DTGN +K  + SW++PYGITFPGKP GRFSDGRV TD+LAK++ +KSPI Y W
Sbjct: 61  VFGDSYADTGNIKKAFSSSWKFPYGITFPGKPAGRFSDGRVATDFLAKFVGIKSPIPYFW 120

Query: 121 RK-VGLGQLKYGMNFAYGGTGVFNTFIMSPNMSTQIDFLQQLIHE-STYTAQDLHYSVAL 180
           +   G  +L+YGMNFAYGGTGVFNT    PNM+TQID  Q ++     Y   +L  SVAL
Sbjct: 121 KDYAGKKRLQYGMNFAYGGTGVFNTQTPLPNMTTQIDIFQNILTTGDIYYPPELTSSVAL 180

Query: 181 ISLAGNDYGVYQATDGSAQSKYKKLSKEFGDFVLSLACWWQGWQPFITKVVNQLEVNLRR 240
           +S+AGNDY           S +  L++   +F             FI +VV+Q EVNLRR
Sbjct: 181 VSVAGNDY-----------SNFIALNRPASEF-----------PAFIKQVVDQTEVNLRR 240

Query: 241 IHGLGVPKIVVTSLEPLGCLPSSTVASSFQQCNATENLLVNFHNLLLQQSVAKLNSEVKD 300
           IH LGV KI V SL+PLGCLP  T  +SFQ+CN T+N LVN HN LLQQ VAKLN+E K 
Sbjct: 241 IHALGVKKIAVPSLQPLGCLPPFTFVTSFQRCNETQNALVNLHNNLLQQVVAKLNNETKQ 300

Query: 301 ASASTFILLDLYSSFMAALNNKEDPLGNVKFENPLKPCCVGISSEYACGNVGANGEKKYT 360
              STFI+LDLY++F+    NK    G+ +FE+PLKPCCVG+S EY CG+V   G KKY 
Sbjct: 301 ---STFIILDLYNAFLTVFKNKGSNPGSTRFESPLKPCCVGVSREYNCGSVDEKGVKKYI 352

Query: 361 ICEDPGAAFFWDEVHPSQNGWFAVYSALQANL 391
           +C++P  AFFWD +HP++ GW +VYS L+ +L
Sbjct: 361 VCDNPKTAFFWDGLHPTEEGWRSVYSVLRESL 352

BLAST of Carg14526 vs. TAIR 10
Match: AT3G09930.1 (GDSL-like Lipase/Acylhydrolase superfamily protein )

HSP 1 Score: 351.3 bits (900), Expect = 1.0e-96
Identity = 179/347 (51.59%), Postives = 230/347 (66.28%), Query Frame = 0

Query: 50  DLSDSNPRKLFVFGDSYVDTGNNRKPAAKSWQYPYGITFPGKPTGRFSDGRVLTDYLAKY 109
           +L    P +LFVFGDSY DTGN RK  + SW+ PYGITFP KP+GRFSDGRV TD+LA+Y
Sbjct: 31  NLKRLRPNRLFVFGDSYADTGNIRKSLSDSWKIPYGITFPQKPSGRFSDGRVATDFLARY 90

Query: 110 LRVKSPIAYKWRK-VGLGQLKYGMNFAYGGTGVFNTFIMS-PNMSTQIDFLQQLIHE-ST 169
           L +KSPI Y W+   G  +L YGMN+AYGGTGVF T     PNM+TQID+ Q+++   + 
Sbjct: 91  LGIKSPIPYTWKDYAGKERLLYGMNYAYGGTGVFKTKDNPLPNMTTQIDYFQRVLAAGNI 150

Query: 170 YTAQDLHYSVALISLAGNDYGVYQATDGSAQSKYKKLSKEFGDFVLSLACWWQGWQPFIT 229
           Y+  DL  S+AL+S+AGNDY  + A           L +   +              F+ 
Sbjct: 151 YSPSDLPSSLALVSVAGNDYATFLA-----------LKRPLTEL-----------PAFMK 210

Query: 230 KVVNQLEVNLRRIHGLGVPKIVVTSLEPLGCLPSSTVASSFQQCNATENLLVNFHNLLLQ 289
           +VV+Q+ VN  RIH LGV KIV+ S++PLGCLPS TV +SFQ+CNAT+N   N HN LL 
Sbjct: 211 QVVDQIAVNAMRIHKLGVNKIVIPSMQPLGCLPSITVFNSFQRCNATDNASTNLHNYLLH 270

Query: 290 QSVAKLNSEVKDASASTFILLDLYSSFMAALNNKEDPLGNVKFENPLKPCCVGISSEYAC 349
           +++A+LN+E K    STF++LD Y++F+    NK    G  +F NPLKPCCVG++S Y C
Sbjct: 271 KAIARLNNETK---PSTFVVLDHYNAFLTVFKNKGPEPGVSRFGNPLKPCCVGVNSSYDC 330

Query: 350 GNVGANGEKKYTICEDPGAAFFWDEVHPSQNGWFAVYSALQANLKQL 394
            NV   GEKKY ICEDP AAFFWD  HPS+ GW +VYS L  +LK +
Sbjct: 331 SNVDEKGEKKYIICEDPKAAFFWDIFHPSEEGWRSVYSVLHKHLKAI 352

BLAST of Carg14526 vs. TAIR 10
Match: AT2G36325.1 (GDSL-like Lipase/Acylhydrolase superfamily protein )

HSP 1 Score: 256.1 bits (653), Expect = 4.4e-68
Identity = 143/334 (42.81%), Postives = 188/334 (56.29%), Query Frame = 0

Query: 56  PRKLFVFGDSYVDTGNNRKPAAKSWQYPYGITFPGKPTGRFSDGRVLTDYLAKYLRVKSP 115
           P KLFVFGDSY DTGN       SW++P GITFPG PTGRFSDGRV TDYLAKY+ V++P
Sbjct: 45  PTKLFVFGDSYADTGNTPFLIVPSWRFPNGITFPGIPTGRFSDGRVSTDYLAKYIGVRTP 104

Query: 116 IAYKWRKVGLGQL--KYGMNFAYGGTGVFNT-FIMSPNMSTQIDFLQQLIHESTYTAQDL 175
           I YKW K G  +L  K GMNFAYGG G F T F + P  S QID  +QL+  + Y+  DL
Sbjct: 105 ITYKWGKYGRPRLAVKRGMNFAYGGAGAFETMFKLVPTASVQIDSFEQLLMRNVYSPADL 164

Query: 176 HYSVALISLAGNDYGVYQATDGSAQSKYKKLSKEFGDFVLSLACWWQGWQPFITKVVNQL 235
           + SVA  S+ GNDY  Y   +GS                       +G      KVV Q+
Sbjct: 165 NSSVAFFSIIGNDYLTYDRRNGSE----------------------EGRSALTRKVVKQI 224

Query: 236 EVNLRRIHGLGVPKIVVTSLEPLGCLPSSTVASSFQQCNATENLLVNFHNLLLQQSVAKL 295
            ++++RI  LGV K++V    P  CLP          C+ T +     HN LL++ + KL
Sbjct: 225 LLDVKRIKDLGVRKVLVALSPPQKCLPKLVTPKG---CD-TNDTSTYLHNSLLRKGLIKL 284

Query: 296 NSEVKDASASTFILLDLYSSFMAALNNKEDPLGNVKFENPLKPCCVGISSEYACGNVGAN 355
           N +  + +  +F+ LDLY++F+    NK    G   F +P K CC      + CG+   +
Sbjct: 285 NDKEINNNDKSFMTLDLYNAFVTIFKNK-GVSGVSTFPDPFKACCATKRGTF-CGDRSLS 344

Query: 356 GEKKYTICEDPGAAFFWDEVHPSQNGWFAVYSAL 387
           G+K YT+C+DP + FFWD VH S  GW +V+S L
Sbjct: 345 GKKLYTLCDDPKSFFFWDNVHISDQGWRSVFSLL 350

BLAST of Carg14526 vs. TAIR 10
Match: AT5G03600.1 (SGNH hydrolase-type esterase superfamily protein )

HSP 1 Score: 247.3 bits (630), Expect = 2.1e-65
Identity = 138/332 (41.57%), Postives = 194/332 (58.43%), Query Frame = 0

Query: 58  KLFVFGDSYVDTGNNRKPAAKSWQYPYGITFPGKPTGRFSDGRVLTDYLAKYLRVKSPIA 117
           KLFVFGDSY DTGN ++   ++W  PYGITFPGKP+GR+ DG + TD+L K L  +SP  
Sbjct: 14  KLFVFGDSYADTGNTKRD-TEAWAIPYGITFPGKPSGRYCDGLIATDFLEKVLGAESPYL 73

Query: 118 YKWRKVGLGQLKYGMNFAYGGTGVFNTFIMS--PNMSTQIDFLQQLIHESTYTAQDLHYS 177
           Y+      G LK GMNFA+GG+ + ++   S  PN++ Q++FL  L+             
Sbjct: 74  YRTHGRDKG-LKRGMNFAFGGSKMLDSSPNSPFPNITAQVNFLVDLVLAGRVYGDITPSD 133

Query: 178 VALISLAGNDYGVYQATDGSAQSKYKKLSKEFGDFVLSLACWWQGWQPFITKVVNQLEVN 237
           V+LIS AG DY  Y   +  A                       G +  + KVV+ L VN
Sbjct: 134 VSLISYAGGDYIYYIDQNRPA----------------------AGLKALVEKVVDNLRVN 193

Query: 238 LRRIHGLGVPKIVVTSLEPLGCLPSSTVASSFQQCNATENLLVNFHNLLLQQSVAKLNSE 297
           +  + GL   KI VTSL+P+GCLPS T ASSF+ CN +++ LV  HN LL++ VAKLN +
Sbjct: 194 MIVLGGLLFKKIAVTSLQPIGCLPSYTSASSFKSCNESQSALVELHNKLLKKVVAKLNEQ 253

Query: 298 VKDASAST-FILLDLYSSFMAALNNKEDPLGNVKFENPLKPCCVGISSEYACGNVGANGE 357
            +       F ++D++++FM  + NK    G+ +F+NP+K CC G      CG   ++G 
Sbjct: 254 SRVMKKEQHFFIIDIHNAFMTVMKNK----GSKRFKNPMKSCCEGY-----CGR-SSDGG 311

Query: 358 KKYTICEDPGAAFFWDEVHPSQNGWFAVYSAL 387
           K YT+C+DP + FFWD VHP+Q GW ++YS L
Sbjct: 314 KLYTLCDDPKSFFFWDAVHPTQEGWRSIYSVL 311

BLAST of Carg14526 vs. TAIR 10
Match: AT5G03590.1 (GDSL-like Lipase/Acylhydrolase superfamily protein )

HSP 1 Score: 149.4 bits (376), Expect = 5.9e-36
Identity = 107/323 (33.13%), Postives = 147/323 (45.51%), Query Frame = 0

Query: 74  KPAAKSWQYPYGITFPGKPTGRFSDGRVLTDYLAKYLRVKSPIAYKWRKVGLGQLKYGMN 133
           KP A SW+ PYGITFPGKP+GR+SDG   TD+L                           
Sbjct: 2   KPTALSWKLPYGITFPGKPSGRYSDGLTATDFL--------------------------- 61

Query: 134 FAYGGTGVFNTFI-MSPNMSTQIDFLQQL-IHESTYTAQ-DLHYSVALISLAGNDYGVYQ 193
               G+ VF++ +  SPN+STQ+ FL  L +    YT   DL  S AL+S +G DY    
Sbjct: 62  ----GSEVFDSPVDRSPNISTQVGFLVNLALARRVYTIDGDLASSYALLSYSGTDY---- 121

Query: 194 ATDGSAQSKYKKLSKEFGDFVLSLACWWQGWQPFITKVVNQLEVNLRRIHGLGVPKIVVT 253
                           +G               FI +  N              P  V  
Sbjct: 122 ----------------YG---------------FIDQNPNM----------AAYPAFVEF 181

Query: 254 SLEPLGCLPSSTVASSFQQCNATENLLVNFHNLLLQQSVAKLNSEVK-DASASTFILLDL 313
            +E +              CN + + LV  HN  L+++VAKLN E K       F+++DL
Sbjct: 182 IVEDI-------------HCNESYSDLVRLHNESLKKAVAKLNKEDKFRTKGDRFVIVDL 226

Query: 314 YSSFMAALNNKEDPLGNVKFENPLKPCCVGISSEYACGNVGANGEKKYTICEDPGAAFFW 373
           + +FM  L  K    GN +F++PLKPCC G      C  +   G KKYT+C DP +AFFW
Sbjct: 242 HKAFMTILEKK----GNKRFKSPLKPCCEG-----DCARMDMKGAKKYTLCNDPKSAFFW 226

Query: 374 DEVHPSQNGWFAVYSALQANLKQ 393
           DE++P+Q GW ++YS L  +L +
Sbjct: 302 DEINPTQEGWRSIYSLLGKSLTE 226