Carg14484 (gene) Silver-seed gourd (SMH-JMG-627) v2

Overview
NameCarg14484
Typegene
OrganismCucurbita argyrosperma subsp. argyrosperma cv. SMH-JMG-627 (Silver-seed gourd (SMH-JMG-627) v2)
DescriptionPectin lyase-like superfamily protein
LocationCarg_Chr18: 8344557 .. 8349114 (-)
RNA-Seq ExpressionCarg14484
SyntenyCarg14484
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideexonCDS
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGAACAAAGGAAGCAAGATTTTAACTCTTTTTCTGGTCTCTTTGCTGGTGTCTTCTTCTGTGGTTCCATTAGAGGCAAGGAAGCATCATCACAACAAGAAGAGCAAGCATCAGAAGCAAAAGGGTGGTGGTAAAGGCAGTGGTTCAAGCCCTGCACCAGCTCCACTTCCTGATTATAATCAAACCCAGTCCACCATTTTTAATATCTTGTCTTTTGGAGCAAAGGGAAATGGTGTTTTTGATGATTCCAAGGTAAAACATTGTCCAAAACATCCTTAAAAAACATATATATGGAGACAGAGCTAAAAATAGTGAACTCTGTAGGCACTTATAGCAGCATGGAAAGCTGCATGCAAGATTCCTGGAGCTACATTACAAATACCTTCGGATTATAAGTTTCTTATCAAACCCATAACTCTAAATGGTCCTTGTATGCCTCAGCTTGTTCTTCAGGTTTGTCTTGTTTTGTTTGCTTATTATGATAAGTTAGTGTTATGATAGTTAATGGGAAGTGGAATTGTAAAGCAGATTGATGGAGATGTATTGGCGCCGCCAAAAGTGAGTTCTTGGTCCAAATCTAGCTTGTTTCAATGGCTGAATTTCAAATGGCTGCATAATTTTACCATTGGTGGCTCTGGGACTGTGGATGGTCAGGGGTTTAATTGGTGGACTGTCTCTACGCTTTATAACAACAAGGTTTGTTCTTTGATTAAGTTATGATAATTGTATTTAAGACAGTGTTTTGCTTAATCATTTCTTGTTTCTGCAGAAGAAAGGGAAGCATGTTCCTGACATGAAGCCTACGGTAAACTTTTCTCACTCTTTCTCCCACTTTATCTATCTCTCTCTCTCTCCCAGTTTAGATTTAGTGTAAGATTCCACGTCGGTTGGAGAGGGGAACGAAACATTCCTTATAGGGGTGTGGAAATCTTTCCATAGTAAACCGCGTTTTAAAATCGTGAGGCTAATGACAATATGTAACGGGCAGAGTGGACAATATCTGCTAGCGGTGGGCTTGGGCTGTTACAAATGGTTCAGAGCCAGACACTGAGCAGTGTGTCAACGAGGACGCTGGACCCCAGGGGGTGGATTGTGAGATCCCACATCGGTTGGAGAGGTAATGGAGCATTCTTTATAAGGATGTGGAAACCTCTCCCTAGTAGACGCGTTTTAAAATTGAAGCTGACGGCGATACGTAACGGGCTAAAGCAGACAATATATGCTAGCGGTGGGTTTTGGTTGTTACAAATGGTATTAGGGCCAAACATTGAGTGGTGTGCCAGCGAGGACGTTGGGCCCCCAAAGGGAGTTGATTTTGAGATCCCACGTCGGTTAGAGAGGGGAATGGAGCATTGCTTATAAGGGTGTGGAAACCTCTCCCTAGCAGACACATTTTAAAACCATGAGGCTGACAACGATATGTAATGGGCCAAAGCGGACAATATATGCTAGTTGTGGGCTTGGGCTGTTGCAAATAGTATCAGAGCTAGATACTGAACGGTGTGCCAGCGAGGACGCTGGCCTCTAAGGGGTGTGGATTGTGAAACCTCTCCTTAGGAGACGCACTTTAAAACCCTGAGGCTGACAGTGATATGTAATGGATGTAACTTAGACAGTTACATTTACCGTTTTTTTTTCCTGAATTTTAATTGGATTTTTTTAATACTTTGTTCATAAATGACAGGCTTTAAGATTTTATTCCAGTTACAATGTGACAGTTCGTGACATCACCATAAGGAACAGTCCACAGTGCCATCTGAAATTCGACAACTCGGGCACCGTCAAAGTCGACAATATCACCATTTCTTCGCCAGAGAGCAGTCCCAACACCGATGGAATTCACTTACAGAACACGCAAGATGTCGAAATTCAACACTCGAACATTGGCTGTGGTATGAACCGAGACAATTCACAGCCCCTTTTTAAATAATATTTTATTGTCATTTCTTTATTTAGAGTCTTTGATAACTATTTAGTTTTGTAATTTAAATATTAACCCTGTTTTTTCTATAAATTTACTGTTTTGTTATCTACTTTTTACCTATCTTTACCTATTTCCTCTGCCATGGAAGCAATAACAGTCCAAGCTTACCGCTAGCAGATATTGTCCTCTTCCTCAAGGTTTTAAAAATGCGTATCTTAGGGGAGGTTTCTACACCCTTATAAAGAATGTTTCGTTCTCCTCCCCAACCGATGTGGGATCTCACAATCCACCTCCCTTTAGGACCCAGCATCCTCGCTCGCACTCGTTCCCTTCTCCAATCGACGTGGGATCTCCCAATCTACCCTCTTCGGAGCCCAGCGTCCTTGCTGACACACCTCCTTGTATCCACCCCCTTTCGAGGCTCAGCCTCTTCGCTAGCACACTGCCCAGTGTTTGGCTCTAATACCATTTGTAACGACCCAAGCCCACAACTAGCAGATATTGTCCTCTTTGGGATTTCCCTTTGGGGGTTTCCCTCAAGATTTTTAAAACGCATCTACTAGGGGGAGATTTCCACACCCTTATAAAGAATGTTTCGTTTTCCTCCTTAACCGAGATGGGATCTCACAATCCACCCCCCTTCAGGGCCCAATATCCTCATCAACACTCGTTCCCTTCTCTAATCGATGTAGGACTCCCCAATCCACCCCCCTTCAGAGCCCAGCGTCCTTGTTGATACACCACCTCATGTCTACCCCCTTCAAGGCTCAGCCTCTTCGCTAGCACACCACCCGGTGTCTAGCTCTGATACCATTTGTAACAGTCCAAGCCCACCGCTAGCAGATATTGTCCTTTTTGGGCTTCCCCTCAAAGTTTTTAAAACGCGTCTGCCAGAGAGAGGTTTCCACACCCATATAAAAAATGTTTCGTTCTCCTCCCCAACCGACGTGGGATCTCACAAAAAAAATGTCTCCGTTTAACAATATTTTAATTGCAATAGCCATAAGGCTACACCAAAAAGAAATAACAAGTAAAGATAAGTGATTAAACAACTTATTTTAAGATTTGTTATTTTATACAGGAGATGACTGTGTTTCCATTCAGACTGGCTGCTCCAACATCCATATTCATCATATCACTTGTGGCCCTGGACATGGCATAAGGTGACTTTTTAATAAACCCAATTCCTGTAAAGAACATTATTTACGGCAGTGATTATAAATTAACCATTTTGATCAGACGGTTCTTGTAAGATCTCATATCGGTTGAGGAGGAGAATGAAGCATTCTTTATAAAGGTGTGGAAATTTCTCCCTAGCAAATGCGTTTTAAAAACCTTAAGGGGAAGCCCGAAAGGAAAAGCTCAAAGAGAACAATATCTGCTAGTGGTGAGCTTGTGCTGTTACAAATGGTATTAAAGTCGGACATCGGGTGGTGTGCCAGCGAAGAGGCTAAGCCCTGAATGGGTGTGGGCACGAGGTGGTGTGTTAGCAAGGACGTTGGGCTCTGAAGAAGGGTGGTTTGTGAGATCCTACATCAGTTGGGAAGGAGAACGAAACATTCTTTATAAGAGTGTGGAAACCTCTCCCTAACCGACGCGTTTTAAAAACCTTGAGGGAAAGTCTAAAGAGGACAATATCTGGTAGTGGTGGGGTTGGGCTGTTACAAATGGTATTAGAGCCAAACACCGGGTGATGTGCCAATGAGGAGGCTGAGCTTCGAGGAGGGGTGGACACGAGGCGGTGTGCCAGCAAGAACGCTGGGCTCAAAGGGGGTAGATTGTGAGATCCCACATCTGTTGGGGAGGATAACGAAGCATTTTTTATAAGGGTGTGGAAACCTCTCCGAAAAATATACATTTTAAAAACCTTAAGGAGAAGTCTGAAAGGGAGAATCCAAAAAGGACAATATTCGCTAGCTGTGGGCTTGGGTTGTTACAAATGATATTAGAACTAGACACCGAGCGGTGTGCCAGCGAAAAGGCTGAACCCGAAAAGGGGTGGAACGAGGTGGTGTGCTAGTAAGGACGCTGGGTCTGGAAGGAGGGTGGATTGTGAGATCCCACATTAGAACGAAACATTCTTTATAAGGGTGTGAAAACCTCTCCCTATACGTATTTTAAAAACCTTAAGGGAAAGTCCGAAAAGGAAAGCCCATAGAAGACAATATCTAGTAGCGGTAGGCTTGCGTTGTTACCGTACTGATTGTCGTGTTTGACGAATATGAACAGGGAGGAGTTGGGTCAGTGAAAAACGTGTCATTCTCCAACATCCAAGTTTCTGATGTAAAGGTACCCATTATGATAGACCAATACTACTGCGACAAGTACAGATGCAAGAACCAAACAGGAGCAGTGGCAATTTCAGGCATAAAATACGATCAAATCATAGGAAGTTACACAGTGCAGCCTATTCACTTAGCTTGCAGCAGCCAAATCCCATGCATAGACGTTGATCTTATAGGTATCCAGCTGAACCCATCTTCAGGGTCCAGAGGCTTTCAGAGCGCTCTGTGTTGGAACTCATATGGCAAATCTCAGGCCCCGCTTGTTCCTTCAAGCATAGACTATTGCTTGAGAAGGGGCAGTGGCGCTGTAAGGAGAATTGCTAGGTCCCATGATCATGCCTGCTACTAA

mRNA sequence

ATGAACAAAGGAAGCAAGATTTTAACTCTTTTTCTGGTCTCTTTGCTGGTGTCTTCTTCTGTGGTTCCATTAGAGGCAAGGAAGCATCATCACAACAAGAAGAGCAAGCATCAGAAGCAAAAGGGTGGTGGTAAAGGCAGTGGTTCAAGCCCTGCACCAGCTCCACTTCCTGATTATAATCAAACCCAGTCCACCATTTTTAATATCTTGTCTTTTGGAGCAAAGGGAAATGGTGTTTTTGATGATTCCAAGGCACTTATAGCAGCATGGAAAGCTGCATGCAAGATTCCTGGAGCTACATTACAAATACCTTCGGATTATAAGTTTCTTATCAAACCCATAACTCTAAATGGTCCTTGTATGCCTCAGCTTGTTCTTCAGATTGATGGAGATGTATTGGCGCCGCCAAAAGTGAGTTCTTGGTCCAAATCTAGCTTGTTTCAATGGCTGAATTTCAAATGGCTGCATAATTTTACCATTGGTGGCTCTGGGACTGTGGATGGTCAGGGGTTTAATTGGTGGACTGTCTCTACGCTTTATAACAACAAGGTACCCATTATGATAGACCAATACTACTGCGACAAGTACAGATGCAAGAACCAAACAGGAGCAGTGGCAATTTCAGGCATAAAATACGATCAAATCATAGGAAGTTACACAGTGCAGCCTATTCACTTAGCTTGCAGCAGCCAAATCCCATGCATAGACGTTGATCTTATAGGTATCCAGCTGAACCCATCTTCAGGGTCCAGAGGCTTTCAGAGCGCTCTGTGTTGGAACTCATATGGCAAATCTCAGGCCCCGCTTGTTCCTTCAAGCATAGACTATTGCTTGAGAAGGGGCAGTGGCGCTGTAAGGAGAATTGCTAGGTCCCATGATCATGCCTGCTACTAA

Coding sequence (CDS)

ATGAACAAAGGAAGCAAGATTTTAACTCTTTTTCTGGTCTCTTTGCTGGTGTCTTCTTCTGTGGTTCCATTAGAGGCAAGGAAGCATCATCACAACAAGAAGAGCAAGCATCAGAAGCAAAAGGGTGGTGGTAAAGGCAGTGGTTCAAGCCCTGCACCAGCTCCACTTCCTGATTATAATCAAACCCAGTCCACCATTTTTAATATCTTGTCTTTTGGAGCAAAGGGAAATGGTGTTTTTGATGATTCCAAGGCACTTATAGCAGCATGGAAAGCTGCATGCAAGATTCCTGGAGCTACATTACAAATACCTTCGGATTATAAGTTTCTTATCAAACCCATAACTCTAAATGGTCCTTGTATGCCTCAGCTTGTTCTTCAGATTGATGGAGATGTATTGGCGCCGCCAAAAGTGAGTTCTTGGTCCAAATCTAGCTTGTTTCAATGGCTGAATTTCAAATGGCTGCATAATTTTACCATTGGTGGCTCTGGGACTGTGGATGGTCAGGGGTTTAATTGGTGGACTGTCTCTACGCTTTATAACAACAAGGTACCCATTATGATAGACCAATACTACTGCGACAAGTACAGATGCAAGAACCAAACAGGAGCAGTGGCAATTTCAGGCATAAAATACGATCAAATCATAGGAAGTTACACAGTGCAGCCTATTCACTTAGCTTGCAGCAGCCAAATCCCATGCATAGACGTTGATCTTATAGGTATCCAGCTGAACCCATCTTCAGGGTCCAGAGGCTTTCAGAGCGCTCTGTGTTGGAACTCATATGGCAAATCTCAGGCCCCGCTTGTTCCTTCAAGCATAGACTATTGCTTGAGAAGGGGCAGTGGCGCTGTAAGGAGAATTGCTAGGTCCCATGATCATGCCTGCTACTAA

Protein sequence

MNKGSKILTLFLVSLLVSSSVVPLEARKHHHNKKSKHQKQKGGGKGSGSSPAPAPLPDYNQTQSTIFNILSFGAKGNGVFDDSKALIAAWKAACKIPGATLQIPSDYKFLIKPITLNGPCMPQLVLQIDGDVLAPPKVSSWSKSSLFQWLNFKWLHNFTIGGSGTVDGQGFNWWTVSTLYNNKVPIMIDQYYCDKYRCKNQTGAVAISGIKYDQIIGSYTVQPIHLACSSQIPCIDVDLIGIQLNPSSGSRGFQSALCWNSYGKSQAPLVPSSIDYCLRRGSGAVRRIARSHDHACY
Homology
[Close]
BLAST of Carg14484 vs. NCBI nr
Match: KAG7012799.1 (putative polygalacturonase, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 617.8 bits (1592), Expect = 4.7e-173
Identity = 297/297 (100.00%), Postives = 297/297 (100.00%), Query Frame = 0 

Query: 1   MNKGSKILTLFLVSLLVSSSVVPLEARKHHHNKKSKHQKQKGGGKGSGSSPAPAPLPDYN 60
           MNKGSKILTLFLVSLLVSSSVVPLEARKHHHNKKSKHQKQKGGGKGSGSSPAPAPLPDYN
Sbjct: 1   MNKGSKILTLFLVSLLVSSSVVPLEARKHHHNKKSKHQKQKGGGKGSGSSPAPAPLPDYN 60

Query: 61  QTQSTIFNILSFGAKGNGVFDDSKALIAAWKAACKIPGATLQIPSDYKFLIKPITLNGPC 120
           QTQSTIFNILSFGAKGNGVFDDSKALIAAWKAACKIPGATLQIPSDYKFLIKPITLNGPC
Sbjct: 61  QTQSTIFNILSFGAKGNGVFDDSKALIAAWKAACKIPGATLQIPSDYKFLIKPITLNGPC 120

Query: 121 MPQLVLQIDGDVLAPPKVSSWSKSSLFQWLNFKWLHNFTIGGSGTVDGQGFNWWTVSTLY 180
           MPQLVLQIDGDVLAPPKVSSWSKSSLFQWLNFKWLHNFTIGGSGTVDGQGFNWWTVSTLY
Sbjct: 121 MPQLVLQIDGDVLAPPKVSSWSKSSLFQWLNFKWLHNFTIGGSGTVDGQGFNWWTVSTLY 180

Query: 181 NNKVPIMIDQYYCDKYRCKNQTGAVAISGIKYDQIIGSYTVQPIHLACSSQIPCIDVDLI 240
           NNKVPIMIDQYYCDKYRCKNQTGAVAISGIKYDQIIGSYTVQPIHLACSSQIPCIDVDLI
Sbjct: 181 NNKVPIMIDQYYCDKYRCKNQTGAVAISGIKYDQIIGSYTVQPIHLACSSQIPCIDVDLI 240

Query: 241 GIQLNPSSGSRGFQSALCWNSYGKSQAPLVPSSIDYCLRRGSGAVRRIARSHDHACY 298
           GIQLNPSSGSRGFQSALCWNSYGKSQAPLVPSSIDYCLRRGSGAVRRIARSHDHACY
Sbjct: 241 GIQLNPSSGSRGFQSALCWNSYGKSQAPLVPSSIDYCLRRGSGAVRRIARSHDHACY 297

[Close]
BLAST of Carg14484 vs. NCBI nr
Match: XP_023542214.1 (polygalacturonase At1g48100 isoform X2 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 547.7 bits (1410), Expect = 6.0e-152
Identity = 296/462 (64.07%), Postives = 296/462 (64.07%), Query Frame = 0 

Query: 1   MNKGSKILTLFLVSLLVSSSVVPLEARKHHHNKKSKHQKQKGGGKGSGSSPAPAPLPDYN 60
           MNKGSKILTLFLVSLLVSSSVVPLEARKHHHNKKSKHQKQKGG KGSGSSPAPAPLPDYN
Sbjct: 1   MNKGSKILTLFLVSLLVSSSVVPLEARKHHHNKKSKHQKQKGGAKGSGSSPAPAPLPDYN 60

Query: 61  QTQSTIFNILSFGAKGNGVFDDSKALIAAWKAACKIPGATLQIPSDYKFLIKPITLNGPC 120
           QTQSTIFNILSFGAKGNGVFDDSKALIAAWKAACKIPGATLQIPSDYKFLIKPITLNGPC
Sbjct: 61  QTQSTIFNILSFGAKGNGVFDDSKALIAAWKAACKIPGATLQIPSDYKFLIKPITLNGPC 120

Query: 121 MPQLVLQIDGDVLAPPKVSSWSKSSLFQWLNFKWLHNFTIGGSGTVDGQGFNWWTVSTLY 180
           MPQLVLQIDGDVLAPPKVSSWSKSSLFQWLNFKWLHNFTIGGSGTVDGQGFNWWTVSTLY
Sbjct: 121 MPQLVLQIDGDVLAPPKVSSWSKSSLFQWLNFKWLHNFTIGGSGTVDGQGFNWWTVSTLY 180

Query: 181 NN---------------------------------------------------------- 240
           NN                                                          
Sbjct: 181 NNKKKGKHVPDMKPTALRFYSSYNVTVRDITIRNSPQCHLKFDNSGTVKVDNITISSPES 240

Query: 241 ------------------------------------------------------------ 298
                                                                       
Sbjct: 241 SPNTDGIHLQNTQDVEIQHSNIGCGDDCVSIQTGCSNIHIHHITCGPGHGISLGGLGKDK 300

[Close]
BLAST of Carg14484 vs. NCBI nr
Match: XP_022944899.1 (polygalacturonase At1g48100 isoform X4 [Cucurbita moschata])

HSP 1 Score: 547.0 bits (1408), Expect = 1.0e-151
Identity = 295/462 (63.85%), Postives = 296/462 (64.07%), Query Frame = 0 

Query: 1   MNKGSKILTLFLVSLLVSSSVVPLEARKHHHNKKSKHQKQKGGGKGSGSSPAPAPLPDYN 60
           MNKGSKILTLFLVSLLVSSSVVPLEARKHHHNKKSKHQKQ+GGGKGSGSSPAPAPLPDYN
Sbjct: 1   MNKGSKILTLFLVSLLVSSSVVPLEARKHHHNKKSKHQKQRGGGKGSGSSPAPAPLPDYN 60

Query: 61  QTQSTIFNILSFGAKGNGVFDDSKALIAAWKAACKIPGATLQIPSDYKFLIKPITLNGPC 120
           QTQSTIFNILSFGAKGNGVFDDSKALIAAWKAACKIPGATLQIPSDYKFLIKPITLNGPC
Sbjct: 61  QTQSTIFNILSFGAKGNGVFDDSKALIAAWKAACKIPGATLQIPSDYKFLIKPITLNGPC 120

Query: 121 MPQLVLQIDGDVLAPPKVSSWSKSSLFQWLNFKWLHNFTIGGSGTVDGQGFNWWTVSTLY 180
           MPQLVLQIDGDVLAPPKVSSWSKSSLFQWLNFKWLHNFTIGG GTVDGQGFNWWTVSTLY
Sbjct: 121 MPQLVLQIDGDVLAPPKVSSWSKSSLFQWLNFKWLHNFTIGGCGTVDGQGFNWWTVSTLY 180

Query: 181 NN---------------------------------------------------------- 240
           NN                                                          
Sbjct: 181 NNKKKGKHIPDMKPTALRFYSSYNVTVRDITIRNSPQCHLKFDNSGTVKVNNITISSPES 240

Query: 241 ------------------------------------------------------------ 298
                                                                       
Sbjct: 241 SPNTDGIHLQNTQDVEIQHSNIGCGDDCVSIQTGCSNIHIHHITCGPGHGISLGGLGKDK 300

[Close]
BLAST of Carg14484 vs. NCBI nr
Match: XP_022966725.1 (polygalacturonase At1g48100 [Cucurbita maxima])

HSP 1 Score: 544.7 bits (1402), Expect = 5.1e-151
Identity = 294/462 (63.64%), Postives = 295/462 (63.85%), Query Frame = 0 

Query: 1   MNKGSKILTLFLVSLLVSSSVVPLEARKHHHNKKSKHQKQKGGGKGSGSSPAPAPLPDYN 60
           MNKGSKILTLFLVSLLVSSSVVPLEARKHHHNKKSKHQKQ+GGGKGSGSSPAPAPLPDYN
Sbjct: 1   MNKGSKILTLFLVSLLVSSSVVPLEARKHHHNKKSKHQKQRGGGKGSGSSPAPAPLPDYN 60

Query: 61  QTQSTIFNILSFGAKGNGVFDDSKALIAAWKAACKIPGATLQIPSDYKFLIKPITLNGPC 120
           QTQSTIFNILSFGAKGNGVFDDSKALIAAW AACKIPGATLQIP DYKFLIKPITLNGPC
Sbjct: 61  QTQSTIFNILSFGAKGNGVFDDSKALIAAWTAACKIPGATLQIPPDYKFLIKPITLNGPC 120

Query: 121 MPQLVLQIDGDVLAPPKVSSWSKSSLFQWLNFKWLHNFTIGGSGTVDGQGFNWWTVSTLY 180
           MPQLVLQIDGDVLAPPKVSSWSKSSLFQWLNFKWLHNFTIGGSGTVDGQGFNWWTVSTLY
Sbjct: 121 MPQLVLQIDGDVLAPPKVSSWSKSSLFQWLNFKWLHNFTIGGSGTVDGQGFNWWTVSTLY 180

Query: 181 NN---------------------------------------------------------- 240
           NN                                                          
Sbjct: 181 NNKKKGKHVPDMKPTALRFYSSYNVTVRDITIINSPQCHLKFDNSGNVKVDNITISSPES 240

Query: 241 ------------------------------------------------------------ 298
                                                                       
Sbjct: 241 SPNTDGIHLQNTQDVEIQHSNIGCGDDCVSIQTGCSNIHIHHINCGPGHGISLGGLGKDK 300

[Close]
BLAST of Carg14484 vs. NCBI nr
Match: XP_023542213.1 (polygalacturonase At1g48100 isoform X1 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 543.1 bits (1398), Expect = 1.5e-150
Identity = 296/463 (63.93%), Postives = 296/463 (63.93%), Query Frame = 0 

Query: 1   MNKGSKILTLFLVSLLVSSSVVPLEARKHHHNKKSKHQKQKGGGKGSGSSPAPAPLPDYN 60
           MNKGSKILTLFLVSLLVSSSVVPLEARKHHHNKKSKHQKQKGG KGSGSSPAPAPLPDYN
Sbjct: 1   MNKGSKILTLFLVSLLVSSSVVPLEARKHHHNKKSKHQKQKGGAKGSGSSPAPAPLPDYN 60

Query: 61  QTQSTIFNILSFGAKGNGVFDDSKALIAAWKAACKIPGATLQIPSDYKFLIKPITLNGPC 120
           QTQSTIFNILSFGAKGNGVFDDSKALIAAWKAACKIPGATLQIPSDYKFLIKPITLNGPC
Sbjct: 61  QTQSTIFNILSFGAKGNGVFDDSKALIAAWKAACKIPGATLQIPSDYKFLIKPITLNGPC 120

Query: 121 MPQLVL-QIDGDVLAPPKVSSWSKSSLFQWLNFKWLHNFTIGGSGTVDGQGFNWWTVSTL 180
           MPQLVL QIDGDVLAPPKVSSWSKSSLFQWLNFKWLHNFTIGGSGTVDGQGFNWWTVSTL
Sbjct: 121 MPQLVLQQIDGDVLAPPKVSSWSKSSLFQWLNFKWLHNFTIGGSGTVDGQGFNWWTVSTL 180

Query: 181 YNN--------------------------------------------------------- 240
           YNN                                                         
Sbjct: 181 YNNKKKGKHVPDMKPTALRFYSSYNVTVRDITIRNSPQCHLKFDNSGTVKVDNITISSPE 240

Query: 241 ------------------------------------------------------------ 298
                                                                       
Sbjct: 241 SSPNTDGIHLQNTQDVEIQHSNIGCGDDCVSIQTGCSNIHIHHITCGPGHGISLGGLGKD 300

[Close]
BLAST of Carg14484 vs. ExPASy Swiss-Prot
Match: Q94AJ5 (Probable polygalacturonase At1g80170 OS=Arabidopsis thaliana OX=3702 GN=At1g80170 PE=1 SV=1)

HSP 1 Score: 112.8 bits (281), Expect = 6.5e-24
Identity = 63/180 (35.00%), Postives = 95/180 (52.78%), Query Frame = 0 

Query: 66  IFNILSFGAKGNGVFDDSKALIAAWKAACKIPGAT-LQIPSDYKFLIKPITLNGPCMPQL 125
           + ++ +FGAKGNGV DD+KA   AWK AC     T + +P +Y  L++PI L+GPC  +L
Sbjct: 52  LLHVGNFGAKGNGVTDDTKAFADAWKTACSSKVKTRILVPENYTCLLRPIDLSGPCKARL 111

Query: 126 VLQIDGDVLAPPKVSSWSKSSLFQWLNFKWLHNFTIGGSGTVDGQGFNWWTVSTLYNNKV 185
            LQI G ++AP     W   +  +WL F  L   T+ G GTV+G G  WW  S  +N+  
Sbjct: 112 TLQISGTIIAPNDPDVWEGLNRRKWLYFHGLSRLTVEGGGTVNGMGQEWWERSCKHNHSN 171

Query: 186 PIMIDQYYCDKYRCKNQTGAVAISGIKYDQIIGSYTVQPIHLACSSQIPCIDVDLIGIQL 245
           P          ++CKN         ++   +I S   Q +H+A +S   C  V + G+++
Sbjct: 172 PCRGAPTALTFHKCKNMR-------VENLNVIDS---QQMHIALTS---CRRVTISGLKV 218

[Close]
BLAST of Carg14484 vs. ExPASy Swiss-Prot
Match: Q949Z1 (Polygalacturonase At1g48100 OS=Arabidopsis thaliana OX=3702 GN=At1g48100 PE=2 SV=1)

HSP 1 Score: 107.5 bits (267), Expect = 2.7e-22
Identity = 107/480 (22.29%), Postives = 156/480 (32.50%), Query Frame = 0 

Query: 1   MNKGSKILTLFLVSLLVSSSVVPLEARKHHHNKKSKH-------------QKQKGGGKGS 60
           M + S IL ++++S ++      + AR  +H  K +H              +        
Sbjct: 1   MRRLSGILVIYIISTVLFGHFTNV-ARARYHYHKGRHGVTHPLPPPPPPPLETANPPDQV 60

Query: 61  GSSPAPAPLPDYNQTQS-TIFNILSFGAKGNGVFDDSKALIAAWKAACKIPGATLQIPSD 120
            S P P+P P    + S  +F++ SFGA G+G  DD+ A   AWKAAC +    +  P  
Sbjct: 61  PSDPYPSPDPAPGDSDSGCVFDVTSFGAVGDGSCDDTAAFQDAWKAACAVESGVVLAPEG 120

Query: 121 YKFLIKPITLNGPCMPQLVLQIDGDVLAPPKVSSW-SKSSLFQWLNFKWLHNFTIGGSGT 180
             F I     +GPC P LV Q+DG ++ P     W  K +  QWL F  L  FT  G GT
Sbjct: 121 GVFKITSTIFSGPCKPGLVFQLDGVLMPPDGPEEWPEKDNKNQWLVFYRLDGFTFSGKGT 180

Query: 181 VDGQGFNWWTVST----------------------------------------------- 240
           V+G G  WW +                                                 
Sbjct: 181 VEGNGQKWWDLPCKPHRGPDGSSSSGPCASPTMIRFFMSNNIEVKGLRIQNSPQFHMKFD 240

Query: 241 ---------------------------------LYNNKVP-------------------- 297
                                            +YN+ V                     
Sbjct: 241 GCQGVLINEIQISSPKLSPNTDGIHLGNTRSVGIYNSVVSNGDDCISIGTGCSDVDIQGV 300

[Close]
BLAST of Carg14484 vs. ExPASy Swiss-Prot
Match: P43212 (Polygalacturonase OS=Cryptomeria japonica OX=3369 PE=1 SV=1)

HSP 1 Score: 106.3 bits (264), Expect = 6.1e-22
Identity = 53/146 (36.30%), Postives = 77/146 (52.74%), Query Frame = 0 

Query: 66  IFNILSFGAKGNGVFDDSKALIAAWKAACKIPGATLQIPSDYKFLIKPITLNGPCMPQLV 125
           IFN+  +GA G+G  D ++A   AW+AACK P A L +P + KF++  +  NGPC P   
Sbjct: 58  IFNVEKYGAVGDGKHDCTEAFSTAWQAACKKPSAMLLVPGNKKFVVNNLFFNGPCQPHFT 117

Query: 126 LQIDGDVLAPPKVSSWSKSSLFQWLNFKWLHNFTIGGSGTVDGQGFNWWTVSTLYNNKVP 185
            ++DG + A    +SW  + +  WL F  L  FT+ G G +DGQG  WW     + N   
Sbjct: 118 FKVDGIIAAYQNPASWKNNRI--WLQFAKLTGFTLMGKGVIDGQGKQWWAGQCKWVNGRE 177

Query: 186 IMIDQYYCDKYRCKNQTGAVAISGIK 212
           I  D+      +    TG + I G+K
Sbjct: 178 ICNDRDRPTAIKFDFSTGLI-IQGLK 200

[Close]
BLAST of Carg14484 vs. ExPASy Swiss-Prot
Match: Q7M1E7 (Polygalacturonase OS=Chamaecyparis obtusa OX=13415 PE=1 SV=1)

HSP 1 Score: 105.5 bits (262), Expect = 1.0e-21
Identity = 43/111 (38.74%), Postives = 65/111 (58.56%), Query Frame = 0 

Query: 64  STIFNILSFGAKGNGVFDDSKALIAAWKAACKIPGATLQIPSDYKFLIKPITLNGPCMPQ 123
           +T+FN+  +GA G+G  D ++A    W AACK   A L +P++ KF +  +   GPC P 
Sbjct: 56  ATVFNVEQYGAVGDGKHDSTEAFATTWNAACKKASAVLLVPANKKFFVNNLVFRGPCQPH 115

Query: 124 LVLQIDGDVLAPPKVSSWSKSSLFQWLNFKWLHNFTIGGSGTVDGQGFNWW 175
           L  ++DG ++A P  + W  S +  WL F  L +F + G+G +DGQG  WW
Sbjct: 116 LSFKVDGTIVAQPDPARWKNSKI--WLQFAQLTDFNLMGTGVIDGQGQQWW 164

[Close]
BLAST of Carg14484 vs. ExPASy Swiss-Prot
Match: P05117 (Polygalacturonase-2 OS=Solanum lycopersicum OX=4081 GN=PG2 PE=1 SV=1)

HSP 1 Score: 94.4 bits (233), Expect = 2.4e-18
Identity = 57/186 (30.65%), Postives = 88/186 (47.31%), Query Frame = 0 

Query: 66  IFNILSFGAKGNGVFDDSKALIAAWKAACKI-PGATLQIPSDYKFLIKPITLNGPCMPQL 125
           + N+LSFGAKG+G   D+ A   AW  AC         +P +  +L+K IT +GPC   +
Sbjct: 75  VINVLSFGAKGDGKTYDNIAFEQAWNEACSSRTPVQFVVPKNKNYLLKQITFSGPCRSSI 134

Query: 126 VLQIDGDVLAPPKVSSWSKSSLFQWLNFKWLHNFTIGGSGTVDGQGFNWWTVSTLYNNKV 185
            ++I G + A  K+S +    L  W+ F  + N  +GG GT++G G  WW  S   N  +
Sbjct: 135 SVKIFGSLEASSKISDYKDRRL--WIAFDSVQNLVVGGGGTINGNGQVWWPSSCKINKSL 194

Query: 186 PIMIDQYYCDKYRCKNQTGAVAISGIKYDQIIGSYTVQPIHLACSSQIPCIDVDLIGIQL 245
           P          + CKN    + ++ +K      S   Q IH+   S   C +V    + +
Sbjct: 195 PCRDAPTALTFWNCKN----LKVNNLK------SKNAQQIHIKFES---CTNVVASNLMI 245

Query: 246 NPSSGS 251
           N S+ S
Sbjct: 255 NASAKS 245

[Close]
BLAST of Carg14484 vs. ExPASy TrEMBL
Match: A0A6J1FZF6 (polygalacturonase At1g48100 isoform X4 OS=Cucurbita moschata OX=3662 GN=LOC111449289 PE=3 SV=1)

HSP 1 Score: 547.0 bits (1408), Expect = 5.0e-152
Identity = 295/462 (63.85%), Postives = 296/462 (64.07%), Query Frame = 0 

Query: 1   MNKGSKILTLFLVSLLVSSSVVPLEARKHHHNKKSKHQKQKGGGKGSGSSPAPAPLPDYN 60
           MNKGSKILTLFLVSLLVSSSVVPLEARKHHHNKKSKHQKQ+GGGKGSGSSPAPAPLPDYN
Sbjct: 1   MNKGSKILTLFLVSLLVSSSVVPLEARKHHHNKKSKHQKQRGGGKGSGSSPAPAPLPDYN 60

Query: 61  QTQSTIFNILSFGAKGNGVFDDSKALIAAWKAACKIPGATLQIPSDYKFLIKPITLNGPC 120
           QTQSTIFNILSFGAKGNGVFDDSKALIAAWKAACKIPGATLQIPSDYKFLIKPITLNGPC
Sbjct: 61  QTQSTIFNILSFGAKGNGVFDDSKALIAAWKAACKIPGATLQIPSDYKFLIKPITLNGPC 120

Query: 121 MPQLVLQIDGDVLAPPKVSSWSKSSLFQWLNFKWLHNFTIGGSGTVDGQGFNWWTVSTLY 180
           MPQLVLQIDGDVLAPPKVSSWSKSSLFQWLNFKWLHNFTIGG GTVDGQGFNWWTVSTLY
Sbjct: 121 MPQLVLQIDGDVLAPPKVSSWSKSSLFQWLNFKWLHNFTIGGCGTVDGQGFNWWTVSTLY 180

Query: 181 NN---------------------------------------------------------- 240
           NN                                                          
Sbjct: 181 NNKKKGKHIPDMKPTALRFYSSYNVTVRDITIRNSPQCHLKFDNSGTVKVNNITISSPES 240

Query: 241 ------------------------------------------------------------ 298
                                                                       
Sbjct: 241 SPNTDGIHLQNTQDVEIQHSNIGCGDDCVSIQTGCSNIHIHHITCGPGHGISLGGLGKDK 300

[Close]
BLAST of Carg14484 vs. ExPASy TrEMBL
Match: A0A6J1HSE8 (polygalacturonase At1g48100 OS=Cucurbita maxima OX=3661 GN=LOC111466350 PE=3 SV=1)

HSP 1 Score: 544.7 bits (1402), Expect = 2.5e-151
Identity = 294/462 (63.64%), Postives = 295/462 (63.85%), Query Frame = 0 

Query: 1   MNKGSKILTLFLVSLLVSSSVVPLEARKHHHNKKSKHQKQKGGGKGSGSSPAPAPLPDYN 60
           MNKGSKILTLFLVSLLVSSSVVPLEARKHHHNKKSKHQKQ+GGGKGSGSSPAPAPLPDYN
Sbjct: 1   MNKGSKILTLFLVSLLVSSSVVPLEARKHHHNKKSKHQKQRGGGKGSGSSPAPAPLPDYN 60

Query: 61  QTQSTIFNILSFGAKGNGVFDDSKALIAAWKAACKIPGATLQIPSDYKFLIKPITLNGPC 120
           QTQSTIFNILSFGAKGNGVFDDSKALIAAW AACKIPGATLQIP DYKFLIKPITLNGPC
Sbjct: 61  QTQSTIFNILSFGAKGNGVFDDSKALIAAWTAACKIPGATLQIPPDYKFLIKPITLNGPC 120

Query: 121 MPQLVLQIDGDVLAPPKVSSWSKSSLFQWLNFKWLHNFTIGGSGTVDGQGFNWWTVSTLY 180
           MPQLVLQIDGDVLAPPKVSSWSKSSLFQWLNFKWLHNFTIGGSGTVDGQGFNWWTVSTLY
Sbjct: 121 MPQLVLQIDGDVLAPPKVSSWSKSSLFQWLNFKWLHNFTIGGSGTVDGQGFNWWTVSTLY 180

Query: 181 NN---------------------------------------------------------- 240
           NN                                                          
Sbjct: 181 NNKKKGKHVPDMKPTALRFYSSYNVTVRDITIINSPQCHLKFDNSGNVKVDNITISSPES 240

Query: 241 ------------------------------------------------------------ 298
                                                                       
Sbjct: 241 SPNTDGIHLQNTQDVEIQHSNIGCGDDCVSIQTGCSNIHIHHINCGPGHGISLGGLGKDK 300

[Close]
BLAST of Carg14484 vs. ExPASy TrEMBL
Match: A0A6J1FZF8 (polygalacturonase At1g48100 isoform X2 OS=Cucurbita moschata OX=3662 GN=LOC111449289 PE=3 SV=1)

HSP 1 Score: 542.7 bits (1397), Expect = 9.3e-151
Identity = 295/473 (62.37%), Postives = 296/473 (62.58%), Query Frame = 0 

Query: 1   MNKGSKILTLFLVSLLVSSSVVPLEARKHHHNKKSKHQKQKGGGKGSGSSPAPAPLPDYN 60
           MNKGSKILTLFLVSLLVSSSVVPLEARKHHHNKKSKHQKQ+GGGKGSGSSPAPAPLPDYN
Sbjct: 1   MNKGSKILTLFLVSLLVSSSVVPLEARKHHHNKKSKHQKQRGGGKGSGSSPAPAPLPDYN 60

Query: 61  QTQSTIFNILSFGAKGNGVFDDSKALIAAWKAACKIPGATLQIPSDYKFLIKPITLNGPC 120
           QTQSTIFNILSFGAKGNGVFDDSKALIAAWKAACKIPGATLQIPSDYKFLIKPITLNGPC
Sbjct: 61  QTQSTIFNILSFGAKGNGVFDDSKALIAAWKAACKIPGATLQIPSDYKFLIKPITLNGPC 120

Query: 121 MPQLVLQIDGDVLAPPKVSSWSKSSLFQWLNFKWLHNFTIGGSGTVDGQGFNWWTVSTLY 180
           MPQLVLQIDGDVLAPPKVSSWSKSSLFQWLNFKWLHNFTIGG GTVDGQGFNWWTVSTLY
Sbjct: 121 MPQLVLQIDGDVLAPPKVSSWSKSSLFQWLNFKWLHNFTIGGCGTVDGQGFNWWTVSTLY 180

Query: 181 NN---------------------------------------------------------- 240
           NN                                                          
Sbjct: 181 NNKKKGKHIPDMKPTALRFYSSYNVTVRDITIRNSPQCHLKFDNSGTVKVNNITISSPES 240

Query: 241 ------------------------------------------------------------ 298
                                                                       
Sbjct: 241 SPNTDGIHLQNTQDVEIQHSNIGCGDDCVSIQTGCSNIHIHHITCGPGHGISTRGLFLAY 300

[Close]
BLAST of Carg14484 vs. ExPASy TrEMBL
Match: A0A6J1FZB5 (polygalacturonase At1g48100 isoform X3 OS=Cucurbita moschata OX=3662 GN=LOC111449289 PE=3 SV=1)

HSP 1 Score: 542.3 bits (1396), Expect = 1.2e-150
Identity = 295/463 (63.71%), Postives = 296/463 (63.93%), Query Frame = 0 

Query: 1   MNKGSKILTLFLVSLLVSSSVVPLEARKHHHNKKSKHQKQKGGGKGSGSSPAPAPLPDYN 60
           MNKGSKILTLFLVSLLVSSSVVPLEARKHHHNKKSKHQKQ+GGGKGSGSSPAPAPLPDYN
Sbjct: 1   MNKGSKILTLFLVSLLVSSSVVPLEARKHHHNKKSKHQKQRGGGKGSGSSPAPAPLPDYN 60

Query: 61  QTQSTIFNILSFGAKGNGVFDDSKALIAAWKAACKIPGATLQIPSDYKFLIKPITLNGPC 120
           QTQSTIFNILSFGAKGNGVFDDSKALIAAWKAACKIPGATLQIPSDYKFLIKPITLNGPC
Sbjct: 61  QTQSTIFNILSFGAKGNGVFDDSKALIAAWKAACKIPGATLQIPSDYKFLIKPITLNGPC 120

Query: 121 MPQLVL-QIDGDVLAPPKVSSWSKSSLFQWLNFKWLHNFTIGGSGTVDGQGFNWWTVSTL 180
           MPQLVL QIDGDVLAPPKVSSWSKSSLFQWLNFKWLHNFTIGG GTVDGQGFNWWTVSTL
Sbjct: 121 MPQLVLQQIDGDVLAPPKVSSWSKSSLFQWLNFKWLHNFTIGGCGTVDGQGFNWWTVSTL 180

Query: 181 YNN--------------------------------------------------------- 240
           YNN                                                         
Sbjct: 181 YNNKKKGKHIPDMKPTALRFYSSYNVTVRDITIRNSPQCHLKFDNSGTVKVNNITISSPE 240

Query: 241 ------------------------------------------------------------ 298
                                                                       
Sbjct: 241 SSPNTDGIHLQNTQDVEIQHSNIGCGDDCVSIQTGCSNIHIHHITCGPGHGISLGGLGKD 300

[Close]
BLAST of Carg14484 vs. ExPASy TrEMBL
Match: A0A6J1FZB4 (polygalacturonase At1g48100 isoform X1 OS=Cucurbita moschata OX=3662 GN=LOC111449289 PE=3 SV=1)

HSP 1 Score: 538.1 bits (1385), Expect = 2.3e-149
Identity = 295/474 (62.24%), Postives = 296/474 (62.45%), Query Frame = 0 

Query: 1   MNKGSKILTLFLVSLLVSSSVVPLEARKHHHNKKSKHQKQKGGGKGSGSSPAPAPLPDYN 60
           MNKGSKILTLFLVSLLVSSSVVPLEARKHHHNKKSKHQKQ+GGGKGSGSSPAPAPLPDYN
Sbjct: 1   MNKGSKILTLFLVSLLVSSSVVPLEARKHHHNKKSKHQKQRGGGKGSGSSPAPAPLPDYN 60

Query: 61  QTQSTIFNILSFGAKGNGVFDDSKALIAAWKAACKIPGATLQIPSDYKFLIKPITLNGPC 120
           QTQSTIFNILSFGAKGNGVFDDSKALIAAWKAACKIPGATLQIPSDYKFLIKPITLNGPC
Sbjct: 61  QTQSTIFNILSFGAKGNGVFDDSKALIAAWKAACKIPGATLQIPSDYKFLIKPITLNGPC 120

Query: 121 MPQLVL-QIDGDVLAPPKVSSWSKSSLFQWLNFKWLHNFTIGGSGTVDGQGFNWWTVSTL 180
           MPQLVL QIDGDVLAPPKVSSWSKSSLFQWLNFKWLHNFTIGG GTVDGQGFNWWTVSTL
Sbjct: 121 MPQLVLQQIDGDVLAPPKVSSWSKSSLFQWLNFKWLHNFTIGGCGTVDGQGFNWWTVSTL 180

Query: 181 YNN--------------------------------------------------------- 240
           YNN                                                         
Sbjct: 181 YNNKKKGKHIPDMKPTALRFYSSYNVTVRDITIRNSPQCHLKFDNSGTVKVNNITISSPE 240

Query: 241 ------------------------------------------------------------ 298
                                                                       
Sbjct: 241 SSPNTDGIHLQNTQDVEIQHSNIGCGDDCVSIQTGCSNIHIHHITCGPGHGISTRGLFLA 300

[Close]
BLAST of Carg14484 vs. TAIR 10
Match: AT3G26610.1 (Pectin lyase-like superfamily protein )

HSP 1 Score: 271.6 bits (693), Expect = 7.7e-73
Identity = 165/468 (35.26%), Postives = 221/468 (47.22%), Query Frame = 0 

Query: 3   KGSKILTLFLVSLLVSS--SVVPLEARKHHHNKKSKHQKQKGGGKGSGSSPAPAPLPDYN 62
           K   IL + L  ++V++  S V +E RK HH KK K + ++        SPAPAP P  N
Sbjct: 5   KSLPILAMLLGVIIVAAAISTVSVEGRK-HHVKKIKPKHRRHSKNTPTGSPAPAPYPSTN 64

Query: 63  QTQSTIFNILSFGAKGNGVFDDSKALIAAWKAACKIPGATLQIPSDYKFLIKPITLNGPC 122
           +    +FNI S+GAKG+GV DDSKAL+ AWKAACK+ G  ++IP+  +FL+K +TL GPC
Sbjct: 65  E---GVFNIFSYGAKGDGVSDDSKALVGAWKAACKVVGGKVEIPAGTQFLVKAVTLQGPC 124

Query: 123 MPQLVLQIDGDVLAPPKVSSWSKSSLFQWLNFKWLHNFTIGGSGTVDGQGFNWWTVSTL- 182
             + V+QI+G ++AP K+ SW  SSLFQWLNFKW+ + TI GSGT++G+G+NWW + T  
Sbjct: 125 KEETVVQIEGILVAPEKIGSWPNSSLFQWLNFKWVSHLTIQGSGTLNGRGYNWWNLDTYQ 184

Query: 183 ------------------------------------------------YNN--------- 242
                                                            NN         
Sbjct: 185 TQTRNKYIPPMKPTALRFYSSNNVTVRDISIVNSPLCHLKFDDSDGVKVNNITISSPENS 244

Query: 243 ------------------------------------------------------------ 297
                                                                       
Sbjct: 245 PNTDGIHLQNTRNVEIQHSNIACGDDCVSIQTGSSNVHIHHINCGPGHGISIGGLGKDKS 304

[Close]
BLAST of Carg14484 vs. TAIR 10
Match: AT1G10640.1 (Pectin lyase-like superfamily protein )

HSP 1 Score: 148.3 bits (373), Expect = 9.9e-36
Identity = 114/463 (24.62%), Postives = 162/463 (34.99%), Query Frame = 0 

Query: 29  HHHNKKSKHQ-KQKGGGKG-SGSSPA--------PAPLPDYNQTQSTIFNILSFGAKGNG 88
           HHH  K K + K K   K    +SP         P PLP   Q  S +FN++ FGAKG+G
Sbjct: 73  HHHKSKPKPKPKLKTPPKAHDNNSPVVSGPPKVQPPPLPP--QKGSQVFNVMDFGAKGDG 132

Query: 89  VFDDSKALIAAWKAACKIPGATLQIPSDYKFLIKPITLNGP-CMPQLVLQIDGDVLAPPK 148
             DD+KA  AAW AACKI  + + +P +Y +L+ PI+ +GP C   +V Q+DG ++AP  
Sbjct: 133 KCDDTKAFEAAWVAACKIEASVMLVPPEYTYLVGPISFSGPYCQANIVFQLDGTIIAPTD 192

Query: 149 VSSWSKSSLFQWLNFKWLHNFTIGGSGTVDGQGFNWW----------------------- 208
             +W K  L  W++F  L    + G G +DG+G  WW                       
Sbjct: 193 SKTWGK-GLMWWIDFTKLKGIKVQGKGVIDGRGSGWWQQDSPFIDSDTKLIVPLNNSANQ 252

Query: 209 --------------------------------TVSTLYNN-------------------- 268
                                           T  T+ N+                    
Sbjct: 253 NPPMPIRSELDERMPSIKPTALRFSGSFGVEVTGITIQNSPQCHLKFDDCVGVVVHDIAV 312

Query: 269 ------------------------------------------------------------ 294
                                                                       
Sbjct: 313 SSPGDSPNTDGIHLQNTKDVLIHSTTLACGDDCISIQTGCSNVFVHNVNCGPGHGISIGS 372

[Close]
BLAST of Carg14484 vs. TAIR 10
Match: AT1G60590.1 (Pectin lyase-like superfamily protein )

HSP 1 Score: 146.7 bits (369), Expect = 2.9e-35
Identity = 107/434 (24.65%), Postives = 150/434 (34.56%), Query Frame = 0 

Query: 51  PAPAPLPDYNQTQSTIFNILSFGAKGNGVFDDSKALIAAWKAACKIPGATLQIPSDYKFL 110
           P P PLP      S  FN+L FGAKG+G+ DD++A  AAW +ACK+  +T+ IP DY FL
Sbjct: 107 PPPTPLPLQPVEDSQQFNVLDFGAKGDGMSDDTQAFEAAWASACKVEASTMIIPPDYIFL 166

Query: 111 IKPITLNGP-CMPQLVLQIDGDVLAPPKVSSWSKSSLFQWLNFKWLHNFTIGGSGTVDGQ 170
           + PI+ +GP C   +V Q++G ++AP    SW    L  W+ F  L   TI G+G +DG+
Sbjct: 167 VGPISFSGPYCQANIVFQLEGMIVAPTDTESWG-GGLMWWIEFTKLSGITIQGNGVIDGR 226

Query: 171 GFNWW------------------------------------------------------- 230
           G  WW                                                       
Sbjct: 227 GTVWWQQDYLSDYPIDDDFKLIVPLNNSVQERPPMPIRSELNWRMPSIKPTALRFYGSID 286

Query: 231 ---TVSTLYNN------------------------------------------------- 290
              T  T+ N+                                                 
Sbjct: 287 VTVTGITIQNSPQCHLKFDNCVKVLVHDVNVSSPGDSPNTDGIHLQNTRDVMIHTTTLAC 346

Query: 291 ------------------------------------------------------------ 294
                                                                       
Sbjct: 347 GDDCISIQTGCSNVYVHNVNCGPGHGISIGSLGKDSTKACVSNITVRDVVMHNTMTGVRI 406

[Close]
BLAST of Carg14484 vs. TAIR 10
Match: AT1G56710.1 (Pectin lyase-like superfamily protein )

HSP 1 Score: 134.4 bits (337), Expect = 1.5e-31
Identity = 103/404 (25.50%), Postives = 141/404 (34.90%), Query Frame = 0 

Query: 50  SPAPAPLPDYNQTQS---TIFNILSFGAKGNGVFDDSKALIAAWKAACKIPG---ATLQI 109
           SP+PAP P YN   +   T+F++ SFGA G+   DD+ A   AW AAC   G   A L +
Sbjct: 28  SPSPAPNPAYNDNDNIAPTVFDVTSFGAIGDCSTDDTSAFKMAWDAACMSTGPKSALLLV 87

Query: 110 PSDYKFLIKPITLNGPCMPQLVLQIDGDVLAPPKVSSWSKSSLFQWLNFKWLHNFTIGGS 169
           P  + FL+KP T NGPC   LVLQIDG +++P    SW  +   QW+ F  ++  +I GS
Sbjct: 88  PYTFCFLVKPTTFNGPCRTNLVLQIDGFIVSPDGPRSWPSNYQRQWMMFYRVNGLSIQGS 147

Query: 170 GTVDGQGFNWW------------------------------------------------- 229
           G ++G+G  WW                                                 
Sbjct: 148 GVINGRGQKWWNLPCKPHKGLNGTTQTGPCDSPVAIRLFQSSKVRIQGINFMNSAQFHVR 207

Query: 230 ------------------------------------------------------------ 279
                                                                       
Sbjct: 208 FDNCSDVVVDSVIIKAPASSPNTDGIHIENTHNVQIRNSMISNGDDCISIGAGCFNVDIK 267

[Close]
BLAST of Carg14484 vs. TAIR 10
Match: AT5G14650.1 (Pectin lyase-like superfamily protein )

HSP 1 Score: 127.1 bits (318), Expect = 2.4e-29
Identity = 106/448 (23.66%), Postives = 157/448 (35.04%), Query Frame = 0 

Query: 8   LTLFLVSLLVSSSVVPLEARKHHHNKKSKHQKQKGGGKGSGSSPAPAPLPDYNQTQSTIF 67
           L   L+  L SS     +AR   + K ++    +GG  G+                    
Sbjct: 11  LNFLLLISLFSSRFGTCDARYSVYWKGNRRSIAEGGSSGT-------------------I 70

Query: 68  NILSFGAKGNGVFDDSKALIAAWKAACKIPGATLQIPSDYKFLIKPITLNG-PCMPQLVL 127
           N+L  GAKG+G  DD+KA   AW+ ACK+  +TL +PS   FL+ P++  G  C  ++V 
Sbjct: 71  NVLDHGAKGDGTSDDTKAFEDAWQVACKVAASTLLVPSGSTFLVGPVSFLGKECKEKIVF 130

Query: 128 QIDGDVLAPPKVSSWSKSSLFQWLNFKWLHNFTIGGSGTVDGQGFNWW------------ 187
           Q++G ++AP   S+W  S L QW+ FK L   TI G G +DG+G  WW            
Sbjct: 131 QLEGKIIAPTSASAWG-SGLLQWIEFKALQGITIKGKGIIDGRGSVWWNDMMGTKMPRTK 190

Query: 188 ------------TVS--------------------------------------------- 247
                       TVS                                             
Sbjct: 191 PTALRFYGSNGVTVSGITIQNSPQTHLKFDNCISIQVSDFTTSSPGDSPNTDGIHLQNSQ 250

Query: 248 --TLYNNKV--------------------------------------------------- 297
              +Y + +                                                   
Sbjct: 251 DAVIYRSTLACGDDCISIQTGCSNINIHDVDCGPGHGISIGGLGKDNTKACVSNITVRDV 310

The following BLAST results are available for this feature:
BLAST of Carg14484 vs. NCBI nr
Match NameE-valueIdentityDescription
KAG7012799.14.7e-173100.00putative polygalacturonase, partial [Cucurbita argyrosperma subsp. argyrosperma][more]
XP_023542214.16.0e-15264.07polygalacturonase At1g48100 isoform X2 [Cucurbita pepo subsp. pepo][more]
XP_022944899.11.0e-15163.85polygalacturonase At1g48100 isoform X4 [Cucurbita moschata][more]
XP_022966725.15.1e-15163.64polygalacturonase At1g48100 [Cucurbita maxima][more]
XP_023542213.11.5e-15063.93polygalacturonase At1g48100 isoform X1 [Cucurbita pepo subsp. pepo][more]
BLAST of Carg14484 vs. ExPASy Swiss-Prot
Match NameE-valueIdentityDescription
Q94AJ56.5e-2435.00Probable polygalacturonase At1g80170 OS=Arabidopsis thaliana OX=3702 GN=At1g8017... [more]
Q949Z12.7e-2222.29Polygalacturonase At1g48100 OS=Arabidopsis thaliana OX=3702 GN=At1g48100 PE=2 SV... [more]
P432126.1e-2236.30Polygalacturonase OS=Cryptomeria japonica OX=3369 PE=1 SV=1[more]
Q7M1E71.0e-2138.74Polygalacturonase OS=Chamaecyparis obtusa OX=13415 PE=1 SV=1[more]
P051172.4e-1830.65Polygalacturonase-2 OS=Solanum lycopersicum OX=4081 GN=PG2 PE=1 SV=1[more]
BLAST of Carg14484 vs. ExPASy TrEMBL
Match NameE-valueIdentityDescription
A0A6J1FZF65.0e-15263.85polygalacturonase At1g48100 isoform X4 OS=Cucurbita moschata OX=3662 GN=LOC11144... [more]
A0A6J1HSE82.5e-15163.64polygalacturonase At1g48100 OS=Cucurbita maxima OX=3661 GN=LOC111466350 PE=3 SV=... [more]
A0A6J1FZF89.3e-15162.37polygalacturonase At1g48100 isoform X2 OS=Cucurbita moschata OX=3662 GN=LOC11144... [more]
A0A6J1FZB51.2e-15063.71polygalacturonase At1g48100 isoform X3 OS=Cucurbita moschata OX=3662 GN=LOC11144... [more]
A0A6J1FZB42.3e-14962.24polygalacturonase At1g48100 isoform X1 OS=Cucurbita moschata OX=3662 GN=LOC11144... [more]
BLAST of Carg14484 vs. TAIR 10
Match NameE-valueIdentityDescription
AT3G26610.17.7e-7335.26Pectin lyase-like superfamily protein [more]
AT1G10640.19.9e-3624.62Pectin lyase-like superfamily protein [more]
AT1G60590.12.9e-3524.65Pectin lyase-like superfamily protein [more]
AT1G56710.11.5e-3125.50Pectin lyase-like superfamily protein [more]
AT5G14650.12.4e-2923.66Pectin lyase-like superfamily protein [more]
InterPro
Analysis Name: InterPro Annotations of Silver-seed gourd (SMH-JMG-627) v2
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR000743Glycoside hydrolase, family 28PFAMPF00295Glyco_hydro_28coord: 99..178
e-value: 8.2E-8
score: 31.7
coord: 179..247
e-value: 2.6E-10
score: 39.9
IPR012334Pectin lyase foldGENE3D2.160.20.10coord: 179..280
e-value: 2.9E-15
score: 58.1
coord: 56..178
e-value: 6.4E-27
score: 96.5
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 26..55
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 27..41
NoneNo IPR availablePANTHERPTHR31375FAMILY NOT NAMEDcoord: 20..177
coord: 181..285
NoneNo IPR availablePANTHERPTHR31375:SF92PECTIN LYASE-LIKE SUPERFAMILY PROTEINcoord: 20..177
NoneNo IPR availablePANTHERPTHR31375:SF92PECTIN LYASE-LIKE SUPERFAMILY PROTEINcoord: 181..285
IPR011050Pectin lyase fold/virulence factorSUPERFAMILY51126Pectin lyase-likecoord: 178..274
IPR011050Pectin lyase fold/virulence factorSUPERFAMILY51126Pectin lyase-likecoord: 60..175

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Carg14484-RACarg14484-RAmRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0005975 carbohydrate metabolic process
molecular_function GO:0004650 polygalacturonase activity