Carg14460 (gene) Silver-seed gourd (SMH-JMG-627) v2

Overview


Name	Carg14460
Type	gene
Organism	Cucurbita argyrosperma subsp. argyrosperma cv. SMH-JMG-627 (Silver-seed gourd (SMH-JMG-627) v2)
Description	U4/U6 small nuclear ribonucleoprotein Prp31 homolog
Location	Carg_Chr18: 8470464 .. 8474111 (-)
RNA-Seq Expression	Carg14460
Synteny	Carg14460

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRpolypeptideCDSthree_prime_UTR

Hold the cursor over a type above to highlight its positions in the sequence below.

AGCTATTCTTCAACTTGGTGCTGAATTGATTACGACCAAGACAACCAGGAAGGCTTTATAATCTTCTGGAGAAGCTTAGAAAATGGTCAGTAACCTTTTTTTTTTTCTGCTTGTATCCATGGAATGTTAGGGTTTCAGCACTTTCTTTTGTATGCTGGTTTAGTTTAGTCTATATGTTTTTTCATGTAATTCAAATTGACTTATGTTGACATTATGAATTTGAGAATATTCTATTAAATATGTAGCTCAATTCTTTATCAGCTGCTAATTCTGTGTTGTTATTCGTTCGTTTTCGGATTTTAAAGTTGCTGAAAGGATATCTTGTATTCAAGAATTTTCTGGATCAGCTTATAATTCATTATTGGTGTAGGGGAACTTGATCTTTTCTCTCTCTTGCATGTAATTCTTTGGAATGAACAAGTTGTGAATCGCTGTATTTGTTTCTGCTTTCTTGTAATGCTATTGTTGTTATCTTTTTATTCATTTTGCTATGATGCTTCTTCCTTCAACTGTATGTTAGAGTTTATCTTGAAAACGTGAATTATCTTGCTTATTGATGATCATATTTTTTCTGGGTTTTGTAGGCTACATTGGCCGATTCTTTTCTAGCAGATCTTGATGAACTCTCTGATGAAGACAATTTTCCGGTGAGCAAATGCAGCATTTTACCTTCCTATCGGGAAAGTAAATTCCATAATCATGTTAGTAGTAGTGTGAACCAATCAATAGTTACTGAATGATTACAGGATGAGGAAGATGTTGACGCTGAAAATATGGAAGAGGATATTGATGGAGACCTTGCCGACCTAGAAAGCCTTAATTATGATGATCTTGATAATGTCTCAAAATTGCAGAAATCACAGAGATACAGTGATATAATGCAGGTTAGTTCACATGAAAGTTTCCCTTATCACTTCGGCATACCTAGTGGCTTTAACGGAAAGGGTTTGGTAAAAGGCCAGTAAATACATTGGAACTCTGTTTTGTCTATTATGCTGACCTTTTGTTGACTGATAAGTTATGGGTTACTATTTAATCATTGGACCTTTTTCTCTGTGATAATTCATTGCTTTTAAATAATGCTATTGGAGTTTTGATGCATTTTCTAATTTACTATTGAAAACCATGTTGTTATTTGAACCACAACTCGTCTCCTTGAAAGGTTAATTTTTATTGCTCATTACATGTTGTATTTCATGATGCTTTATCTCCAAATCATTAAGTTTCTGCAGACTGATATGTTATAGGTTATCGGATTTTACAGAAAGTGGAAGATGCACTACAGAAGGATTCTAATATTTCAAATCAAGGATTAGTATTGGAAGATGATCCTGAGTACCAACTGATTGTGGAGTGTAATGCTTTGTCGGTGGATATTGAGAATGAAATTATTATTATTCACAATTTTATACGTGATAAATATCGATTGAAATTTCCAGAGCTTGAATCACTTGTGCACCACCCAATTGATTATGCTCGAGTTGTTAAGAAGATTGGCAATGAAATGGATTTGACTCTTGTAGACTTAGAAGGGCTTTTACCCTCTGCTGTCATTATGGTTGTCTCAGTTACAGCATCTACCACAAGTGGCAAGCCACTTCCAGAGGAAATTCTCCAGAAAACAATTGATGCATGTGACCGGGCTCTTGCTTTAGATTCAGCAAAGAAAATGGTTCTTAACTTTGTTGAAAGTAGAATGGGGTATATTGCACCAAATCTTTCAGCCATAGTTGGAAGTGCTGTTGCAGCAAAACTAATGGGAACTGCTGGTGGTCTTGGTTCTTTAGCTAAGATGCCTGCTTGCAATGTCCAGCTTCTTGGTGCGAAGAAAAAGAACCTTGCTGGGTTTTCCACAGCAACTTCACAGTTTCGGGTAGGTTACATTGAGCAAACAGAGATATTCCAATCAACACCTCCCCCTTTGAAGATGCGTGCTTGTCGACTTGTATCTGCAAAATCAACACTTGCAGCACGAGTTGACTCCACCAGGGGCGATCCTACTGGGAAGACTGGAAGAGTTTTTAAAGATGAGATCCTTAAGAAAATTGAGAAGTGGCAAGAACCTCCACCAGCTAAACAACCAAAACCTCTTCCGGTCCCTGATTCTGAACCCAAAAAGAAGAGAGGTGGCCGTCGACTAAGGAAGATGAAGGAAAGGTACTGATATGTTTTCCGTCTCTCTAACAATCAAAGGTTTAATTGTTTCACTTTTCGGTTGATCATGGATCAATATGACTTCCCTTGTTAGATATGCAACAACAGAGATGAGGAAGCTAGCCAACAGGATGCAGTTTGGGGTGCCTGAAGAGAGTTCTTTAGGCAAGGCTTTCTTTCAGTTTTATAATAATCTGGATTGGTTAAATTCGTATTCTTGCGTCTGCTTCATTCCATTTACATTAATATGAGATGCAATGCTGTGCTGTTCGATATGCTTGAGTTGCCAAGTTGTTTGTCTTGTGAACCAGAAAACAGTCGTAATTGCAAGTAATACTACCATAATCATCTTGCGTCTTTCCTCTCTCAGGAATATTGGCATGCCAGAAGTAGTTGTATTTTCATCTGTATTTAGAAATTTGTTCTGGATGGAATAAGTAATTATTTGAAGCATTCTCCAATAAGATTCTGAATAAAGATTTCACTCTTTTCATCCTTTCATACAACAGGAGATGGATTAGGGGAAGGGTATGGAATGCTTGGTCAGGCTGGGAGTGGCAAGCTGCGCGTGTCAGCTGCTCAGAGCAAGCTTGCCGCAAAAGTTGTTAAGAAGTAAGTCAAGTCATTGAGCTGATATTGATATTTTCATACCCCTGGAAAATAGAAAATGAAAAATAGAAGTAGATGAGAAAGAGAAGATACTTGCAGCCTAAAATCTGCCCGTTTATCTGTTTTTGTTGACTTATTTTCTCAATTTACTTTTAGGTTCAAGGAAAAACGTTATGGAAGCAGTGGTGCTACGTCCGGCCTGACGTCCAGTTTAGCATTTACACCTGTACAAGTCAGTATTTCTTGCCTTCCTCACCATTTCCTCCTATGTTATCTATGTTATAGAAAGTAATTGTTTTTTGCATGATAATGCCTGGATACTTAATCTTGAGGTTGTGACAATTTCATGTTTTCGTTGCAGGGGATTGAGCTGTCGAATCCTCAGGCTCATTTGAATCAATTAGGCGGTGGAACTCAAAGCACCTACTTTTCTGAAACGGGAACATTTTCAAAGATCAGGAAAAACTAAGTCATTATACAATCATGCATCCTGTTTATGGTCGTGCTGTAACCCTACATAGATATGCCTTACGATGACGCTTTGTAGTGCCCTGTTAAGCGAAAGCCACAGGATTTTTTTTTTTTTTTGTTTCTATAAGACGAAGGTTATTATTTCCCCTTGTTTTGATTATCTTTCATCATTTTTGGGATATAAATGACCACTTAAAAGTGTTAGTCTTTTGGCAGGACTTGATATTATAGTGGTCCGCCTTACACTTTACCTGGTGGTTTTGTCTTTTAGTGGCCCGCCTTACACTTCAAGAAGTTAGTCTCTTAATTATTGCCATCAAATTATAGTTAGTACTTCATCAAAATCGTCTGATTTCCTTGAGGTGAAAATCATCAAAACTTGCACATCCCACTACTCCCCTGAGGAAGTTTAGG

mRNA sequence

AGCTATTCTTCAACTTGGTGCTGAATTGATTACGACCAAGACAACCAGGAAGGCTTTATAATCTTCTGGAGAAGCTTAGAAAATGGCTACATTGGCCGATTCTTTTCTAGCAGATCTTGATGAACTCTCTGATGAAGACAATTTTCCGGATGAGGAAGATGTTGACGCTGAAAATATGGAAGAGGATATTGATGGAGACCTTGCCGACCTAGAAAGCCTTAATTATGATGATCTTGATAATGTCTCAAAATTGCAGAAATCACAGAGATACAGTGATATAATGCAGAAAGTGGAAGATGCACTACAGAAGGATTCTAATATTTCAAATCAAGGATTAGTATTGGAAGATGATCCTGAGTACCAACTGATTGTGGAGTGTAATGCTTTGTCGGTGGATATTGAGAATGAAATTATTATTATTCACAATTTTATACGTGATAAATATCGATTGAAATTTCCAGAGCTTGAATCACTTGTGCACCACCCAATTGATTATGCTCGAGTTGTTAAGAAGATTGGCAATGAAATGGATTTGACTCTTGTAGACTTAGAAGGGCTTTTACCCTCTGCTGTCATTATGGTTGTCTCAGTTACAGCATCTACCACAAGTGGCAAGCCACTTCCAGAGGAAATTCTCCAGAAAACAATTGATGCATGTGACCGGGCTCTTGCTTTAGATTCAGCAAAGAAAATGGTTCTTAACTTTGTTGAAAGTAGAATGGGGTATATTGCACCAAATCTTTCAGCCATAGTTGGAAGTGCTGTTGCAGCAAAACTAATGGGAACTGCTGGTGGTCTTGGTTCTTTAGCTAAGATGCCTGCTTGCAATGTCCAGCTTCTTGGTGCGAAGAAAAAGAACCTTGCTGGGTTTTCCACAGCAACTTCACAGTTTCGGGTAGGTTACATTGAGCAAACAGAGATATTCCAATCAACACCTCCCCCTTTGAAGATGCGTGCTTGTCGACTTGTATCTGCAAAATCAACACTTGCAGCACGAGTTGACTCCACCAGGGGCGATCCTACTGGGAAGACTGGAAGAGTTTTTAAAGATGAGATCCTTAAGAAAATTGAGAAGTGGCAAGAACCTCCACCAGCTAAACAACCAAAACCTCTTCCGGTCCCTGATTCTGAACCCAAAAAGAAGAGAGGTGGCCGTCGACTAAGGAAGATGAAGGAAAGATATGCAACAACAGAGATGAGGAAGCTAGCCAACAGGATGCAGTTTGGGGTGCCTGAAGAGAGTTCTTTAGGCAAGGCTTTCTTTCAGTTTTATAATAATCTGGATTGGTTAAATTCGTATTCTTGCAAAACAGTCGTAATTGCAAGAGATGGATTAGGGGAAGGGTATGGAATGCTTGGTCAGGCTGGGAGTGGCAAGCTGCGCGTGTCAGCTGCTCAGAGCAAGCTTGCCGCAAAAGTTGTTAAGAAGTTCAAGGAAAAACGTTATGGAAGCAGTGGTGCTACGTCCGGCCTGACGTCCAGTTTAGCATTTACACCTGTACAAGGGATTGAGCTGTCGAATCCTCAGGCTCATTTGAATCAATTAGGCGGTGGAACTCAAAGCACCTACTTTTCTGAAACGGGAACATTTTCAAAGATCAGGAAAAACTAAGTCATTATACAATCATGCATCCTGTTTATGGTCGTGCTGTAACCCTACATAGATATGCCTTACGATGACGCTTTGTAGTGCCCTGTTAAGCGAAAGCCACAGGATTTTTTTTTTTTTTTGTTTCTATAAGACGAAGGTTATTATTTCCCCTTGTTTTGATTATCTTTCATCATTTGACTTGATATTATAGTGGTCCGCCTTACACTTTACCTGGTGGTTTTGTCTTTTAGTGGCCCGCCTTACACTTCAAGAAGTTAGTCTCTTAATTATTGCCATCAAATTATAGTTAGTACTTCATCAAAATCGTCTGATTTCCTTGAGGTGAAAATCATCAAAACTTGCACATCCCACTACTCCCCTGAGGAAGTTTAGG

Coding sequence (CDS)

ATGGCTACATTGGCCGATTCTTTTCTAGCAGATCTTGATGAACTCTCTGATGAAGACAATTTTCCGGATGAGGAAGATGTTGACGCTGAAAATATGGAAGAGGATATTGATGGAGACCTTGCCGACCTAGAAAGCCTTAATTATGATGATCTTGATAATGTCTCAAAATTGCAGAAATCACAGAGATACAGTGATATAATGCAGAAAGTGGAAGATGCACTACAGAAGGATTCTAATATTTCAAATCAAGGATTAGTATTGGAAGATGATCCTGAGTACCAACTGATTGTGGAGTGTAATGCTTTGTCGGTGGATATTGAGAATGAAATTATTATTATTCACAATTTTATACGTGATAAATATCGATTGAAATTTCCAGAGCTTGAATCACTTGTGCACCACCCAATTGATTATGCTCGAGTTGTTAAGAAGATTGGCAATGAAATGGATTTGACTCTTGTAGACTTAGAAGGGCTTTTACCCTCTGCTGTCATTATGGTTGTCTCAGTTACAGCATCTACCACAAGTGGCAAGCCACTTCCAGAGGAAATTCTCCAGAAAACAATTGATGCATGTGACCGGGCTCTTGCTTTAGATTCAGCAAAGAAAATGGTTCTTAACTTTGTTGAAAGTAGAATGGGGTATATTGCACCAAATCTTTCAGCCATAGTTGGAAGTGCTGTTGCAGCAAAACTAATGGGAACTGCTGGTGGTCTTGGTTCTTTAGCTAAGATGCCTGCTTGCAATGTCCAGCTTCTTGGTGCGAAGAAAAAGAACCTTGCTGGGTTTTCCACAGCAACTTCACAGTTTCGGGTAGGTTACATTGAGCAAACAGAGATATTCCAATCAACACCTCCCCCTTTGAAGATGCGTGCTTGTCGACTTGTATCTGCAAAATCAACACTTGCAGCACGAGTTGACTCCACCAGGGGCGATCCTACTGGGAAGACTGGAAGAGTTTTTAAAGATGAGATCCTTAAGAAAATTGAGAAGTGGCAAGAACCTCCACCAGCTAAACAACCAAAACCTCTTCCGGTCCCTGATTCTGAACCCAAAAAGAAGAGAGGTGGCCGTCGACTAAGGAAGATGAAGGAAAGATATGCAACAACAGAGATGAGGAAGCTAGCCAACAGGATGCAGTTTGGGGTGCCTGAAGAGAGTTCTTTAGGCAAGGCTTTCTTTCAGTTTTATAATAATCTGGATTGGTTAAATTCGTATTCTTGCAAAACAGTCGTAATTGCAAGAGATGGATTAGGGGAAGGGTATGGAATGCTTGGTCAGGCTGGGAGTGGCAAGCTGCGCGTGTCAGCTGCTCAGAGCAAGCTTGCCGCAAAAGTTGTTAAGAAGTTCAAGGAAAAACGTTATGGAAGCAGTGGTGCTACGTCCGGCCTGACGTCCAGTTTAGCATTTACACCTGTACAAGGGATTGAGCTGTCGAATCCTCAGGCTCATTTGAATCAATTAGGCGGTGGAACTCAAAGCACCTACTTTTCTGAAACGGGAACATTTTCAAAGATCAGGAAAAACTAA

Protein sequence

MATLADSFLADLDELSDEDNFPDEEDVDAENMEEDIDGDLADLESLNYDDLDNVSKLQKSQRYSDIMQKVEDALQKDSNISNQGLVLEDDPEYQLIVECNALSVDIENEIIIIHNFIRDKYRLKFPELESLVHHPIDYARVVKKIGNEMDLTLVDLEGLLPSAVIMVVSVTASTTSGKPLPEEILQKTIDACDRALALDSAKKMVLNFVESRMGYIAPNLSAIVGSAVAAKLMGTAGGLGSLAKMPACNVQLLGAKKKNLAGFSTATSQFRVGYIEQTEIFQSTPPPLKMRACRLVSAKSTLAARVDSTRGDPTGKTGRVFKDEILKKIEKWQEPPPAKQPKPLPVPDSEPKKKRGGRRLRKMKERYATTEMRKLANRMQFGVPEESSLGKAFFQFYNNLDWLNSYSCKTVVIARDGLGEGYGMLGQAGSGKLRVSAAQSKLAAKVVKKFKEKRYGSSGATSGLTSSLAFTPVQGIELSNPQAHLNQLGGGTQSTYFSETGTFSKIRKN

Homology

[Close]

BLAST of Carg14460 vs. NCBI nr
Match: KAG7012823.1 (U4/U6 small nuclear ribonucleoprotein Prp31-like protein [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 965.3 bits (2494), Expect = 2.1e-277
Identity = 509/509 (100.00%), Postives = 509/509 (100.00%), Query Frame = 0


Query: 1   MATLADSFLADLDELSDEDNFPDEEDVDAENMEEDIDGDLADLESLNYDDLDNVSKLQKS 60
           MATLADSFLADLDELSDEDNFPDEEDVDAENMEEDIDGDLADLESLNYDDLDNVSKLQKS
Sbjct: 1   MATLADSFLADLDELSDEDNFPDEEDVDAENMEEDIDGDLADLESLNYDDLDNVSKLQKS 60

Query: 61  QRYSDIMQKVEDALQKDSNISNQGLVLEDDPEYQLIVECNALSVDIENEIIIIHNFIRDK 120
           QRYSDIMQKVEDALQKDSNISNQGLVLEDDPEYQLIVECNALSVDIENEIIIIHNFIRDK
Sbjct: 61  QRYSDIMQKVEDALQKDSNISNQGLVLEDDPEYQLIVECNALSVDIENEIIIIHNFIRDK 120

Query: 121 YRLKFPELESLVHHPIDYARVVKKIGNEMDLTLVDLEGLLPSAVIMVVSVTASTTSGKPL 180
           YRLKFPELESLVHHPIDYARVVKKIGNEMDLTLVDLEGLLPSAVIMVVSVTASTTSGKPL
Sbjct: 121 YRLKFPELESLVHHPIDYARVVKKIGNEMDLTLVDLEGLLPSAVIMVVSVTASTTSGKPL 180

Query: 181 PEEILQKTIDACDRALALDSAKKMVLNFVESRMGYIAPNLSAIVGSAVAAKLMGTAGGLG 240
           PEEILQKTIDACDRALALDSAKKMVLNFVESRMGYIAPNLSAIVGSAVAAKLMGTAGGLG
Sbjct: 181 PEEILQKTIDACDRALALDSAKKMVLNFVESRMGYIAPNLSAIVGSAVAAKLMGTAGGLG 240

Query: 241 SLAKMPACNVQLLGAKKKNLAGFSTATSQFRVGYIEQTEIFQSTPPPLKMRACRLVSAKS 300
           SLAKMPACNVQLLGAKKKNLAGFSTATSQFRVGYIEQTEIFQSTPPPLKMRACRLVSAKS
Sbjct: 241 SLAKMPACNVQLLGAKKKNLAGFSTATSQFRVGYIEQTEIFQSTPPPLKMRACRLVSAKS 300

Query: 301 TLAARVDSTRGDPTGKTGRVFKDEILKKIEKWQEPPPAKQPKPLPVPDSEPKKKRGGRRL 360
           TLAARVDSTRGDPTGKTGRVFKDEILKKIEKWQEPPPAKQPKPLPVPDSEPKKKRGGRRL
Sbjct: 301 TLAARVDSTRGDPTGKTGRVFKDEILKKIEKWQEPPPAKQPKPLPVPDSEPKKKRGGRRL 360

Query: 361 RKMKERYATTEMRKLANRMQFGVPEESSLGKAFFQFYNNLDWLNSYSCKTVVIARDGLGE 420
           RKMKERYATTEMRKLANRMQFGVPEESSLGKAFFQFYNNLDWLNSYSCKTVVIARDGLGE
Sbjct: 361 RKMKERYATTEMRKLANRMQFGVPEESSLGKAFFQFYNNLDWLNSYSCKTVVIARDGLGE 420

Query: 421 GYGMLGQAGSGKLRVSAAQSKLAAKVVKKFKEKRYGSSGATSGLTSSLAFTPVQGIELSN 480
           GYGMLGQAGSGKLRVSAAQSKLAAKVVKKFKEKRYGSSGATSGLTSSLAFTPVQGIELSN
Sbjct: 421 GYGMLGQAGSGKLRVSAAQSKLAAKVVKKFKEKRYGSSGATSGLTSSLAFTPVQGIELSN 480

Query: 481 PQAHLNQLGGGTQSTYFSETGTFSKIRKN 510
           PQAHLNQLGGGTQSTYFSETGTFSKIRKN
Sbjct: 481 PQAHLNQLGGGTQSTYFSETGTFSKIRKN 509

[Close]

BLAST of Carg14460 vs. NCBI nr
Match: XP_022945419.1 (U4/U6 small nuclear ribonucleoprotein Prp31 homolog [Cucurbita moschata] >XP_022945420.1 U4/U6 small nuclear ribonucleoprotein Prp31 homolog [Cucurbita moschata] >KAG6573747.1 U4/U6 small nuclear ribonucleoprotein Prp31-like protein, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 897.5 bits (2318), Expect = 5.3e-257
Identity = 484/509 (95.09%), Postives = 484/509 (95.09%), Query Frame = 0


Query: 1   MATLADSFLADLDELSDEDNFPDEEDVDAENMEEDIDGDLADLESLNYDDLDNVSKLQKS 60
           MATLADSFLADLDELSDEDNFPDEEDVDAENMEEDIDGDLADLESLNYDDLDNVSKLQKS
Sbjct: 1   MATLADSFLADLDELSDEDNFPDEEDVDAENMEEDIDGDLADLESLNYDDLDNVSKLQKS 60

Query: 61  QRYSDIMQKVEDALQKDSNISNQGLVLEDDPEYQLIVECNALSVDIENEIIIIHNFIRDK 120
           QRYSDIMQKVEDALQKDSNISNQGLVLEDDPEYQLIVECNALSVDIENEIIIIHNFIRDK
Sbjct: 61  QRYSDIMQKVEDALQKDSNISNQGLVLEDDPEYQLIVECNALSVDIENEIIIIHNFIRDK 120

Query: 121 YRLKFPELESLVHHPIDYARVVKKIGNEMDLTLVDLEGLLPSAVIMVVSVTASTTSGKPL 180
           YRLKFPELESLVHHPIDYARVVKKIGNEMDLTLVDLEGLLPSAVIMVVSVTASTTSGKPL
Sbjct: 121 YRLKFPELESLVHHPIDYARVVKKIGNEMDLTLVDLEGLLPSAVIMVVSVTASTTSGKPL 180

Query: 181 PEEILQKTIDACDRALALDSAKKMVLNFVESRMGYIAPNLSAIVGSAVAAKLMGTAGGLG 240
           PEEILQKTIDACDRALALDSAKKMVLNFVESRMGYIAPNLSAIVGSAVAAKLMGTAGGLG
Sbjct: 181 PEEILQKTIDACDRALALDSAKKMVLNFVESRMGYIAPNLSAIVGSAVAAKLMGTAGGLG 240

Query: 241 SLAKMPACNVQLLGAKKKNLAGFSTATSQFRVGYIEQTEIFQSTPPPLKMRACRLVSAKS 300
           SLAKMPACNVQLLGAKKKNLAGFSTATSQFRVGYIEQTEIFQSTPPPLKMRACRLVSAKS
Sbjct: 241 SLAKMPACNVQLLGAKKKNLAGFSTATSQFRVGYIEQTEIFQSTPPPLKMRACRLVSAKS 300

Query: 301 TLAARVDSTRGDPTGKTGRVFKDEILKKIEKWQEPPPAKQPKPLPVPDSEPKKKRGGRRL 360
           TLAARVDSTRGDPTGKTGRVFKDEILKKIEKWQEPPPAKQPKPLPVPDSEPKKKRGGRRL
Sbjct: 301 TLAARVDSTRGDPTGKTGRVFKDEILKKIEKWQEPPPAKQPKPLPVPDSEPKKKRGGRRL 360

Query: 361 RKMKERYATTEMRKLANRMQFGVPEESSLGKAFFQFYNNLDWLNSYSCKTVVIARDGLGE 420
           RKMKERYATTEMRKLANRMQFGVPEESSLG                         DGLGE
Sbjct: 361 RKMKERYATTEMRKLANRMQFGVPEESSLG-------------------------DGLGE 420

Query: 421 GYGMLGQAGSGKLRVSAAQSKLAAKVVKKFKEKRYGSSGATSGLTSSLAFTPVQGIELSN 480
           GYGMLGQAGSGKLRVSAAQSKLAAKVVKKFKEKRYGSSGATSGLTSSLAFTPVQGIELSN
Sbjct: 421 GYGMLGQAGSGKLRVSAAQSKLAAKVVKKFKEKRYGSSGATSGLTSSLAFTPVQGIELSN 480

Query: 481 PQAHLNQLGGGTQSTYFSETGTFSKIRKN 510
           PQAHLNQLGGGTQSTYFSETGTFSKIRKN
Sbjct: 481 PQAHLNQLGGGTQSTYFSETGTFSKIRKN 484

[Close]

BLAST of Carg14460 vs. NCBI nr
Match: XP_022966885.1 (U4/U6 small nuclear ribonucleoprotein Prp31 homolog [Cucurbita maxima])

HSP 1 Score: 887.5 bits (2292), Expect = 5.5e-254
Identity = 479/509 (94.11%), Postives = 481/509 (94.50%), Query Frame = 0


Query: 1   MATLADSFLADLDELSDEDNFPDEEDVDAENMEEDIDGDLADLESLNYDDLDNVSKLQKS 60
           MATLADSFLADLDELSDEDNFPDEEDVDAENMEEDIDGDLADLESLNYDDLDNVSKLQKS
Sbjct: 1   MATLADSFLADLDELSDEDNFPDEEDVDAENMEEDIDGDLADLESLNYDDLDNVSKLQKS 60

Query: 61  QRYSDIMQKVEDALQKDSNISNQGLVLEDDPEYQLIVECNALSVDIENEIIIIHNFIRDK 120
           QRYSDIMQKVEDALQKDSNISNQGLVLEDDPEYQLIVECNALSVDIENEIIIIHNFIRDK
Sbjct: 61  QRYSDIMQKVEDALQKDSNISNQGLVLEDDPEYQLIVECNALSVDIENEIIIIHNFIRDK 120

Query: 121 YRLKFPELESLVHHPIDYARVVKKIGNEMDLTLVDLEGLLPSAVIMVVSVTASTTSGKPL 180
           YRLKFPELESLVHHPIDYARVVKKIGNEMDLTLVDLEGLLPSAVIMVVSVTASTTSGKPL
Sbjct: 121 YRLKFPELESLVHHPIDYARVVKKIGNEMDLTLVDLEGLLPSAVIMVVSVTASTTSGKPL 180

Query: 181 PEEILQKTIDACDRALALDSAKKMVLNFVESRMGYIAPNLSAIVGSAVAAKLMGTAGGLG 240
           PEEILQKTIDACDRALALDSAKKMVLNFVESRMGYIAPNLSAIVGSAVAAKLMGTAGGL 
Sbjct: 181 PEEILQKTIDACDRALALDSAKKMVLNFVESRMGYIAPNLSAIVGSAVAAKLMGTAGGLA 240

Query: 241 SLAKMPACNVQLLGAKKKNLAGFSTATSQFRVGYIEQTEIFQSTPPPLKMRACRLVSAKS 300
           SLAKMPACNVQLLGAKKKNLAGFSTATSQFR+GYIEQTEIFQSTPP LKMRACRLVSAKS
Sbjct: 241 SLAKMPACNVQLLGAKKKNLAGFSTATSQFRLGYIEQTEIFQSTPPHLKMRACRLVSAKS 300

Query: 301 TLAARVDSTRGDPTGKTGRVFKDEILKKIEKWQEPPPAKQPKPLPVPDSEPKKKRGGRRL 360
           TLAARVDSTRGDPTGKTGRVFKDEILKKIEKWQEPPPAKQPKPLPVPDSEPKKKRGGRRL
Sbjct: 301 TLAARVDSTRGDPTGKTGRVFKDEILKKIEKWQEPPPAKQPKPLPVPDSEPKKKRGGRRL 360

Query: 361 RKMKERYATTEMRKLANRMQFGVPEESSLGKAFFQFYNNLDWLNSYSCKTVVIARDGLGE 420
           RKMKERYATTEMRKLANRMQFGVPEESSLG                         DGLGE
Sbjct: 361 RKMKERYATTEMRKLANRMQFGVPEESSLG-------------------------DGLGE 420

Query: 421 GYGMLGQAGSGKLRVSAAQSKLAAKVVKKFKEKRYGSSGATSGLTSSLAFTPVQGIELSN 480
           GYGMLGQAGSGKLRVSAAQSKLAAKVVKKFKEKRYGSSGATSGLTSSLAFTPVQGIELSN
Sbjct: 421 GYGMLGQAGSGKLRVSAAQSKLAAKVVKKFKEKRYGSSGATSGLTSSLAFTPVQGIELSN 480

Query: 481 PQAHLNQLGGGTQSTYFSETGTFSKIRKN 510
           PQAH+NQLG GTQSTYFSETGTFSKIRKN
Sbjct: 481 PQAHMNQLGSGTQSTYFSETGTFSKIRKN 484

[Close]

BLAST of Carg14460 vs. NCBI nr
Match: XP_022151138.1 (U4/U6 small nuclear ribonucleoprotein Prp31 homolog isoform X1 [Momordica charantia])

HSP 1 Score: 875.9 bits (2262), Expect = 1.6e-250
Identity = 472/509 (92.73%), Postives = 479/509 (94.11%), Query Frame = 0


Query: 1   MATLADSFLADLDELSDEDNFPDEEDVDAENMEEDIDGDLADLESLNYDDLDNVSKLQKS 60
           MATLADSFLADLDELSDEDNF DEEDVDAENMEEDIDGDLADLESLNYDDLD+VSKLQK+
Sbjct: 1   MATLADSFLADLDELSDEDNFLDEEDVDAENMEEDIDGDLADLESLNYDDLDSVSKLQKT 60

Query: 61  QRYSDIMQKVEDALQKDSNISNQGLVLEDDPEYQLIVECNALSVDIENEIIIIHNFIRDK 120
           QRY+DIMQKVE ALQKDSNISNQGLVLEDDPEYQLIVECN+LSVDIENEIIIIHNFIRDK
Sbjct: 61  QRYNDIMQKVEGALQKDSNISNQGLVLEDDPEYQLIVECNSLSVDIENEIIIIHNFIRDK 120

Query: 121 YRLKFPELESLVHHPIDYARVVKKIGNEMDLTLVDLEGLLPSAVIMVVSVTASTTSGKPL 180
           YRLKFPELESLVHHPIDYARVVKKIGNEMDLTLVDLEGLLPSAVIMVVSVTASTTSGKPL
Sbjct: 121 YRLKFPELESLVHHPIDYARVVKKIGNEMDLTLVDLEGLLPSAVIMVVSVTASTTSGKPL 180

Query: 181 PEEILQKTIDACDRALALDSAKKMVLNFVESRMGYIAPNLSAIVGSAVAAKLMGTAGGLG 240
           PEEILQKTIDACDRALALDSAKK VLNFVESRMGYIAPNLSAIVGSAVAAKLMGTAGGL 
Sbjct: 181 PEEILQKTIDACDRALALDSAKKKVLNFVESRMGYIAPNLSAIVGSAVAAKLMGTAGGLA 240

Query: 241 SLAKMPACNVQLLGAKKKNLAGFSTATSQFRVGYIEQTEIFQSTPPPLKMRACRLVSAKS 300
           +LAKMPACNVQLLGAKKKNLAGFSTATSQFRVGYIEQTEIFQSTPPPLKMRACRL++AKS
Sbjct: 241 ALAKMPACNVQLLGAKKKNLAGFSTATSQFRVGYIEQTEIFQSTPPPLKMRACRLIAAKS 300

Query: 301 TLAARVDSTRGDPTGKTGRVFKDEILKKIEKWQEPPPAKQPKPLPVPDSEPKKKRGGRRL 360
           TLAARVDSTRGDPTGKTGRVFKDEILKKIEKWQEPPPAKQPKPLPVPDSEPKKKRGGRRL
Sbjct: 301 TLAARVDSTRGDPTGKTGRVFKDEILKKIEKWQEPPPAKQPKPLPVPDSEPKKKRGGRRL 360

Query: 361 RKMKERYATTEMRKLANRMQFGVPEESSLGKAFFQFYNNLDWLNSYSCKTVVIARDGLGE 420
           RKMKERYATTEMRKLANRMQFGVPEESSLG                         DGLGE
Sbjct: 361 RKMKERYATTEMRKLANRMQFGVPEESSLG-------------------------DGLGE 420

Query: 421 GYGMLGQAGSGKLRVSAAQSKLAAKVVKKFKEKRYGSSGATSGLTSSLAFTPVQGIELSN 480
           GYGMLGQAGSGKLRVSAAQSKLAAKVVKKFKEKRYGSSGATSGLTSSLAFTPVQGIELSN
Sbjct: 421 GYGMLGQAGSGKLRVSAAQSKLAAKVVKKFKEKRYGSSGATSGLTSSLAFTPVQGIELSN 480

Query: 481 PQAHLNQLGGGTQSTYFSETGTFSKIRKN 510
           PQAHLNQLG GTQSTYFSETGTFSKIRKN
Sbjct: 481 PQAHLNQLGSGTQSTYFSETGTFSKIRKN 484

[Close]

BLAST of Carg14460 vs. NCBI nr
Match: XP_022956658.1 (U4/U6 small nuclear ribonucleoprotein Prp31 homolog isoform X1 [Cucurbita moschata] >XP_023004221.1 U4/U6 small nuclear ribonucleoprotein Prp31 homolog isoform X1 [Cucurbita maxima] >XP_023526610.1 U4/U6 small nuclear ribonucleoprotein Prp31 homolog isoform X1 [Cucurbita pepo subsp. pepo] >KAG6601489.1 U4/U6 small nuclear ribonucleoprotein Prp31-like protein, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 872.5 bits (2253), Expect = 1.8e-249
Identity = 469/509 (92.14%), Postives = 477/509 (93.71%), Query Frame = 0


Query: 1   MATLADSFLADLDELSDEDNFPDEEDVDAENMEEDIDGDLADLESLNYDDLDNVSKLQKS 60
           MATLADSFLADLDELSDEDNF DEED DAENMEEDIDGDLADLESLNYDDLD+VSKLQK+
Sbjct: 1   MATLADSFLADLDELSDEDNFLDEEDADAENMEEDIDGDLADLESLNYDDLDSVSKLQKT 60

Query: 61  QRYSDIMQKVEDALQKDSNISNQGLVLEDDPEYQLIVECNALSVDIENEIIIIHNFIRDK 120
           QRY+DIMQKV DAL+ DSN+SNQG VLEDDPEYQLIVECNALSVDIENEIIIIHNFIRDK
Sbjct: 61  QRYNDIMQKVADALEMDSNVSNQGFVLEDDPEYQLIVECNALSVDIENEIIIIHNFIRDK 120

Query: 121 YRLKFPELESLVHHPIDYARVVKKIGNEMDLTLVDLEGLLPSAVIMVVSVTASTTSGKPL 180
           YRLKFPELESLVHHPIDYARVVKKIGNEMDLTLVDLEGLLPSAVIMVVSVTASTTSGKPL
Sbjct: 121 YRLKFPELESLVHHPIDYARVVKKIGNEMDLTLVDLEGLLPSAVIMVVSVTASTTSGKPL 180

Query: 181 PEEILQKTIDACDRALALDSAKKMVLNFVESRMGYIAPNLSAIVGSAVAAKLMGTAGGLG 240
           PEEILQKTIDACDRAL LDSAKKMVLNFVESRMGYIAPNLSAIVGSAVAAKLMGTAGGL 
Sbjct: 181 PEEILQKTIDACDRALTLDSAKKMVLNFVESRMGYIAPNLSAIVGSAVAAKLMGTAGGLV 240

Query: 241 SLAKMPACNVQLLGAKKKNLAGFSTATSQFRVGYIEQTEIFQSTPPPLKMRACRLVSAKS 300
           +LAKMPACNVQLLGAKKKNLAGFSTATSQFRVGYIEQTEIFQSTPPPLKMRACRL++AKS
Sbjct: 241 ALAKMPACNVQLLGAKKKNLAGFSTATSQFRVGYIEQTEIFQSTPPPLKMRACRLIAAKS 300

Query: 301 TLAARVDSTRGDPTGKTGRVFKDEILKKIEKWQEPPPAKQPKPLPVPDSEPKKKRGGRRL 360
           TLAARVDSTRGDPTGKTGRVFKDEILKKIEKWQEPPPAKQPKPLPVPDSEPKKKRGGRRL
Sbjct: 301 TLAARVDSTRGDPTGKTGRVFKDEILKKIEKWQEPPPAKQPKPLPVPDSEPKKKRGGRRL 360

Query: 361 RKMKERYATTEMRKLANRMQFGVPEESSLGKAFFQFYNNLDWLNSYSCKTVVIARDGLGE 420
           RKMKERYATTEMRKLANRMQFGVPEESSLG                         DGLGE
Sbjct: 361 RKMKERYATTEMRKLANRMQFGVPEESSLG-------------------------DGLGE 420

Query: 421 GYGMLGQAGSGKLRVSAAQSKLAAKVVKKFKEKRYGSSGATSGLTSSLAFTPVQGIELSN 480
           GYGMLGQAGSGKLRVSAAQSKLAAKVVKKFKEKRYGSSGATSGLTSSLAFTPVQGIELSN
Sbjct: 421 GYGMLGQAGSGKLRVSAAQSKLAAKVVKKFKEKRYGSSGATSGLTSSLAFTPVQGIELSN 480

Query: 481 PQAHLNQLGGGTQSTYFSETGTFSKIRKN 510
           PQAHLNQLGGGTQSTYFSETGTFSKIRKN
Sbjct: 481 PQAHLNQLGGGTQSTYFSETGTFSKIRKN 484

[Close]

BLAST of Carg14460 vs. ExPASy Swiss-Prot
Match: Q8RXN6 (U4/U6 small nuclear ribonucleoprotein Prp31 homolog OS=Arabidopsis thaliana OX=3702 GN=PRP31 PE=1 SV=1)

HSP 1 Score: 681.4 bits (1757), Expect = 7.8e-195
Identity = 374/510 (73.33%), Postives = 419/510 (82.16%), Query Frame = 0


Query: 1   MATLADSFLADLDELSDEDNFPDEEDVDAENMEEDIDGDLADLESLNYDDLDNVSKLQKS 60
           MATL DSFLADLDELSD +   DE D D    EED+D D+ADLE+LNYDDLDNVSKLQKS
Sbjct: 1   MATLEDSFLADLDELSDNEAELDENDGDVGKEEEDVDMDMADLETLNYDDLDNVSKLQKS 60

Query: 61  QRYSDIMQKVEDALQKDSNISNQGLVLEDDPEYQLIVECNALSVDIENEIIIIHNFIRDK 120
           QRY+DIM KVE+AL KDS+ + +G VLEDDPEY+LIV+CN LSVDIENEI+I+HNFI+DK
Sbjct: 61  QRYADIMHKVEEALGKDSDGAEKGTVLEDDPEYKLIVDCNQLSVDIENEIVIVHNFIKDK 120

Query: 121 YRLKFPELESLVHHPIDYARVVKKIGNEMDLTLVDLEGLLPSAVIMVVSVTASTTSGKPL 180
           Y+LKF ELESLVHHPIDYA VVKKIGNE DL LVDL  LLPSA+IMVVSVTA TT G  L
Sbjct: 121 YKLKFQELESLVHHPIDYACVVKKIGNETDLALVDLADLLPSAIIMVVSVTALTTKGSAL 180

Query: 181 PEEILQKTIDACDRALALDSAKKMVLNFVESRMGYIAPNLSAIVGSAVAAKLMGTAGGLG 240
           PE++LQK ++ACDRAL LDSA+K VL FVES+MG IAPNLSAIVGSAVAAKLMGTAGGL 
Sbjct: 181 PEDVLQKVLEACDRALDLDSARKKVLEFVESKMGSIAPNLSAIVGSAVAAKLMGTAGGLS 240

Query: 241 SLAKMPACNVQLLGAKKKNLAGFSTATSQFRVGYIEQTEIFQSTPPPLKMRACRLVSAKS 300
           +LAKMPACNVQ+LG K+KNLAGFS+ATSQ RVGY+EQTEI+QSTPP L+ RA RLV+AKS
Sbjct: 241 ALAKMPACNVQVLGHKRKNLAGFSSATSQSRVGYLEQTEIYQSTPPGLQARAGRLVAAKS 300

Query: 301 TLAARVDSTRGDPTGKTGRVFKDEILKKIEKWQEPPPAKQPKPLPVPDSEPKKKRGGRRL 360
           TLAARVD+TRGDP G +G+ F++EI KKIEKWQEPPPA+QPKPLPVPDSEPKK+RGGRRL
Sbjct: 301 TLAARVDATRGDPLGISGKAFREEIRKKIEKWQEPPPARQPKPLPVPDSEPKKRRGGRRL 360

Query: 361 RKMKERYATTEMRKLANRMQFGVPEESSLGKAFFQFYNNLDWLNSYSCKTVVIARDGLGE 420
           RKMKERY  T+MRKLANRM FG PEESSLG                         DGLGE
Sbjct: 361 RKMKERYQVTDMRKLANRMAFGTPEESSLG-------------------------DGLGE 420

Query: 421 GYGMLGQAGSGKLRVSAAQSKLA--AKVVKKFKEKRYGSSGATSGLTSSLAFTPVQGIEL 480
           GYGMLGQAGS +LRVS+  SKL   AKV KK KE++Y     TSGLTSSLAFTPVQGIEL
Sbjct: 421 GYGMLGQAGSNRLRVSSVPSKLKINAKVAKKLKERQYAGGATTSGLTSSLAFTPVQGIEL 480

Query: 481 SNPQAHLNQLGGGTQSTYFSETGTFSKIRK 509
            NPQ  L  LG GTQSTYFSE+GTFSK++K
Sbjct: 481 CNPQQALG-LGSGTQSTYFSESGTFSKLKK 484

[Close]

BLAST of Carg14460 vs. ExPASy Swiss-Prot
Match: Q8CCF0 (U4/U6 small nuclear ribonucleoprotein Prp31 OS=Mus musculus OX=10090 GN=Prpf31 PE=1 SV=3)

HSP 1 Score: 374.8 bits (961), Expect = 1.6e-102
Identity = 234/521 (44.91%), Postives = 333/521 (63.92%), Query Frame = 0


Query: 3   TLADSFLADLDELSDED---NFPDEEDVDA-ENMEEDIDGDLADLESLNYDDLDNVSKLQ 62
           +LAD  LADL+E ++E+   ++ +EE+  A E+++E+   DL+       D + +++KL 
Sbjct: 2   SLADELLADLEEAAEEEEGGSYGEEEEEPAIEDVQEETQLDLSG------DSVKSIAKLW 61

Query: 63  KSQRYSDIMQKVEDALQKDSNISNQGLVLEDDPEYQLIVECNALSVDIENEIIIIHNFIR 122
            S+ +++IM K+E+ + K +N+S     +E  PEY++IV+ N L+V+IENE+ IIH FIR
Sbjct: 62  DSKMFAEIMMKIEEYISKQANVSEVMGPVEAAPEYRVIVDANNLTVEIENELNIIHKFIR 121

Query: 123 DKYRLKFPELESLVHHPIDYARVVKKIGNEMD--LTLVDLEGLLPSAVIMVVSVTASTTS 182
           DKY  +FPELESLV + +DY R VK++GN +D      +L+ +L +A IMVVSVTASTT 
Sbjct: 122 DKYSKRFPELESLVPNALDYIRTVKELGNSLDKCKNNENLQQILTNATIMVVSVTASTTQ 181

Query: 183 GKPLPEEILQKTIDACDRALALDSAKKMVLNFVESRMGYIAPNLSAIVGSAVAAKLMGTA 242
           G+ L +E L++  +ACD AL L+++K  +  +VESRM +IAPNLS I+G++ AAK+MG A
Sbjct: 182 GQQLSDEELERLEEACDMALELNASKHRIYEYVESRMSFIAPNLSIIIGASTAAKIMGVA 241

Query: 243 GGLGSLAKMPACNVQLLGAKKKNLAGFSTATSQFRVGYIEQTEIFQSTPPPLKMRACRLV 302
           GGL +L+KMPACN+ LLGA++K L+GFS+ +     GYI  ++I QS PP L+ +A RLV
Sbjct: 242 GGLTNLSKMPACNIMLLGAQRKTLSGFSSTSVLPHTGYIYHSDIVQSLPPDLRRKAARLV 301

Query: 303 SAKSTLAARVDSTRGDPTGKTGRVFKDEILKKIEKWQEPPPAKQPKPLPVPDSEPKKKRG 362
           +AK TLAARVDS      GK G   KDEI +K +KWQEPPP KQ KPLP P    +KKRG
Sbjct: 302 AAKCTLAARVDSFHESTEGKVGYELKDEIERKFDKWQEPPPVKQVKPLPAPLDGQRKKRG 361

Query: 363 GRRLRKMKERYATTEMRKLANRMQFGVPEESSLGKAFFQFYNNLDWLNSYSCKTVVIARD 422
           GRR RKMKER   TE+RK ANRM FG  EE +                          ++
Sbjct: 362 GRRYRKMKERLGLTEIRKQANRMSFGEIEEDAY-------------------------QE 421

Query: 423 GLGEGYGMLGQAGSGKLR---VSAAQSKLAAKVVKKFKEKR---YGSSGA----TSGLTS 482
            LG   G LG++GSG++R   V+ A     +K +++  +K+   YG        +SG  S
Sbjct: 422 DLGFSLGHLGKSGSGRVRQTQVNEATKARISKTLQRTLQKQSVVYGGKSTIRDRSSGTAS 481

Query: 483 SLAFTPVQGIELSNPQAHLNQLGGGTQSTYFSETGTFSKIR 508
           S+AFTP+QG+E+ NPQA   ++    Q  YFS    F K++
Sbjct: 482 SVAFTPLQGLEIVNPQAAEKKVAEANQK-YFSSMAEFLKVK 490

[Close]

BLAST of Carg14460 vs. ExPASy Swiss-Prot
Match: Q5U5C5 (U4/U6 small nuclear ribonucleoprotein Prp31 OS=Xenopus laevis OX=8355 GN=prpf31 PE=2 SV=1)

HSP 1 Score: 373.2 bits (957), Expect = 4.6e-102
Identity = 237/520 (45.58%), Postives = 328/520 (63.08%), Query Frame = 0


Query: 3   TLADSFLADLDELS--DEDNFPDEEDVDA-ENMEEDIDGDLADLESLNYDDLDNVSKLQK 62
           +LAD  LADL+E +  +E+N  DE+D++  E ++E++  D      LN + + +++KL  
Sbjct: 2   SLADELLADLEEAAEEEEENLIDEDDLETIEEVDEEMQVD------LNAESVKSIAKLSD 61

Query: 63  SQRYSDIMQKVEDALQKDSNISNQGLVLEDDPEYQLIVECNALSVDIENEIIIIHNFIRD 122
           S+ +S+I+ K+E  +QK    S     +E  PEY++IV+ N L+V+IENE+ IIH FIRD
Sbjct: 62  SKLFSEILLKIEGYIQKQPKASEVMGPVEAAPEYKVIVDANNLTVEIENELNIIHKFIRD 121

Query: 123 KYRLKFPELESLVHHPIDYARVVKKIGNEMD--LTLVDLEGLLPSAVIMVVSVTASTTSG 182
           KY  +FPELESLV + +DY R VK++GN +D      +L+ +L +A IMVVSVTASTT G
Sbjct: 122 KYSKRFPELESLVPNALDYIRTVKELGNNLDKCKNNENLQQILTNATIMVVSVTASTTQG 181

Query: 183 KPLPEEILQKTIDACDRALALDSAKKMVLNFVESRMGYIAPNLSAIVGSAVAAKLMGTAG 242
           + L +E L++  +ACD AL L+ +K  +  +VESRM +IAPNLS IVG++ AAK+MG AG
Sbjct: 182 QQLTDEELERIEEACDMALELNQSKHRIYEYVESRMSFIAPNLSIIVGASTAAKIMGIAG 241

Query: 243 GLGSLAKMPACNVQLLGAKKKNLAGFSTATSQFRVGYIEQTEIFQSTPPPLKMRACRLVS 302
           GL +L+KMPACNV LLGA++K L GFS+ +     GYI  +EI QS P  L  +A RLVS
Sbjct: 242 GLTNLSKMPACNVMLLGAQRKTLTGFSSTSVLPHTGYIYHSEIVQSLPSDLHRKAARLVS 301

Query: 303 AKSTLAARVDSTRGDPTGKTGRVFKDEILKKIEKWQEPPPAKQPKPLPVPDSEPKKKRGG 362
           AK TLA+RVDS   +P GK G   K+EI +K +KWQEPPP KQ KPLP P    +KKRGG
Sbjct: 302 AKCTLASRVDSFHENPEGKIGYDLKEEIERKFDKWQEPPPVKQVKPLPAPLDGQRKKRGG 361

Query: 363 RRLRKMKERYATTEMRKLANRMQFGVPEESSLGKAFFQFYNNLDWLNSYSCKTVVIARDG 422
           RR RKMKER   TE+RK ANRM FG  EE +                          ++ 
Sbjct: 362 RRYRKMKERLGLTEIRKQANRMSFGEIEEDAY-------------------------QED 421

Query: 423 LGEGYGMLGQAGSGKLR---VSAAQSKLAAKVVKKFKEKR---YGSSGA----TSGLTSS 482
           LG   G LG++GSG++R   V+ A     +K +++  +K+   YG        +SG  SS
Sbjct: 422 LGFSLGHLGKSGSGRIRQAQVNEATKARISKTLQRTLQKQSVVYGGKSTVRDRSSGTASS 481

Query: 483 LAFTPVQGIELSNPQAHLNQLGGGTQSTYFSETGTFSKIR 508
           +AFTP+QG+E+ NPQA   ++    Q  YFS    F K++
Sbjct: 482 VAFTPLQGLEIVNPQAAEKKVAEANQK-YFSSMAEFLKVK 489

[Close]

BLAST of Carg14460 vs. ExPASy Swiss-Prot
Match: Q8WWY3 (U4/U6 small nuclear ribonucleoprotein Prp31 OS=Homo sapiens OX=9606 GN=PRPF31 PE=1 SV=2)

HSP 1 Score: 371.7 bits (953), Expect = 1.3e-101
Identity = 234/521 (44.91%), Postives = 330/521 (63.34%), Query Frame = 0


Query: 3   TLADSFLADLDELSDED---NFPDEEDVDA-ENMEEDIDGDLADLESLNYDDLDNVSKLQ 62
           +LAD  LADL+E ++E+   ++ +EE+  A E+++E+   DL+       D +  ++KL 
Sbjct: 2   SLADELLADLEEAAEEEEGGSYGEEEEEPAIEDVQEETQLDLSG------DSVKTIAKLW 61

Query: 63  KSQRYSDIMQKVEDALQKDSNISNQGLVLEDDPEYQLIVECNALSVDIENEIIIIHNFIR 122
            S+ +++IM K+E+ + K +  S     +E  PEY++IV+ N L+V+IENE+ IIH FIR
Sbjct: 62  DSKMFAEIMMKIEEYISKQAKASEVMGPVEAAPEYRVIVDANNLTVEIENELNIIHKFIR 121

Query: 123 DKYRLKFPELESLVHHPIDYARVVKKIGNEMD--LTLVDLEGLLPSAVIMVVSVTASTTS 182
           DKY  +FPELESLV + +DY R VK++GN +D      +L+ +L +A IMVVSVTASTT 
Sbjct: 122 DKYSKRFPELESLVPNALDYIRTVKELGNSLDKCKNNENLQQILTNATIMVVSVTASTTQ 181

Query: 183 GKPLPEEILQKTIDACDRALALDSAKKMVLNFVESRMGYIAPNLSAIVGSAVAAKLMGTA 242
           G+ L EE L++  +ACD AL L+++K  +  +VESRM +IAPNLS I+G++ AAK+MG A
Sbjct: 182 GQQLSEEELERLEEACDMALELNASKHRIYEYVESRMSFIAPNLSIIIGASTAAKIMGVA 241

Query: 243 GGLGSLAKMPACNVQLLGAKKKNLAGFSTATSQFRVGYIEQTEIFQSTPPPLKMRACRLV 302
           GGL +L+KMPACN+ LLGA++K L+GFS+ +     GYI  ++I QS PP L+ +A RLV
Sbjct: 242 GGLTNLSKMPACNIMLLGAQRKTLSGFSSTSVLPHTGYIYHSDIVQSLPPDLRRKAARLV 301

Query: 303 SAKSTLAARVDSTRGDPTGKTGRVFKDEILKKIEKWQEPPPAKQPKPLPVPDSEPKKKRG 362
           +AK TLAARVDS      GK G   KDEI +K +KWQEPPP KQ KPLP P    +KKRG
Sbjct: 302 AAKCTLAARVDSFHESTEGKVGYELKDEIERKFDKWQEPPPVKQVKPLPAPLDGQRKKRG 361

Query: 363 GRRLRKMKERYATTEMRKLANRMQFGVPEESSLGKAFFQFYNNLDWLNSYSCKTVVIARD 422
           GRR RKMKER   TE+RK ANRM FG  EE +                          ++
Sbjct: 362 GRRYRKMKERLGLTEIRKQANRMSFGEIEEDAY-------------------------QE 421

Query: 423 GLGEGYGMLGQAGSGKLR---VSAAQSKLAAKVVKKFKEKR---YGSSGA----TSGLTS 482
            LG   G LG++GSG++R   V+ A     +K +++  +K+   YG        +SG  S
Sbjct: 422 DLGFSLGHLGKSGSGRVRQTQVNEATKARISKTLQRTLQKQSVVYGGKSTIRDRSSGTAS 481

Query: 483 SLAFTPVQGIELSNPQAHLNQLGGGTQSTYFSETGTFSKIR 508
           S+AFTP+QG+E+ NPQA   ++    Q  YFS    F K++
Sbjct: 482 SVAFTPLQGLEIVNPQAAEKKVAEANQK-YFSSMAEFLKVK 490

[Close]

BLAST of Carg14460 vs. ExPASy Swiss-Prot
Match: Q6NVP6 (U4/U6 small nuclear ribonucleoprotein Prp31 OS=Xenopus tropicalis OX=8364 GN=prpf31 PE=2 SV=1)

HSP 1 Score: 368.6 bits (945), Expect = 1.1e-100
Identity = 234/520 (45.00%), Postives = 327/520 (62.88%), Query Frame = 0


Query: 3   TLADSFLADLDELS--DEDNFPDEEDVDA-ENMEEDIDGDLADLESLNYDDLDNVSKLQK 62
           +LAD  LADL+E +  +E+N  DE+D++  E ++E++  D      LN + + +++KL  
Sbjct: 2   SLADELLADLEEAAEEEEENLIDEDDLETIEEVQEEMQVD------LNAESVKSIAKLSD 61

Query: 63  SQRYSDIMQKVEDALQKDSNISNQGLVLEDDPEYQLIVECNALSVDIENEIIIIHNFIRD 122
           S+ +S+I+ K++  ++K    S     +E  PEY++IV+ N L+V+IENE+ IIH FIRD
Sbjct: 62  SKLFSEILLKIDGYIKKQPKASEVMGPVEAAPEYKVIVDANNLTVEIENELNIIHKFIRD 121

Query: 123 KYRLKFPELESLVHHPIDYARVVKKIGNEMD--LTLVDLEGLLPSAVIMVVSVTASTTSG 182
           KY  +FPELESLV + +DY R VK++GN +D      +L+ +L +A IMVVSVTASTT G
Sbjct: 122 KYSKRFPELESLVPNALDYIRTVKELGNNLDKCKNNENLQQILTNATIMVVSVTASTTQG 181

Query: 183 KPLPEEILQKTIDACDRALALDSAKKMVLNFVESRMGYIAPNLSAIVGSAVAAKLMGTAG 242
           + L +E L++  +ACD AL L+ +K  +  +VESRM +IAPNLS IVG++ AAK+MG AG
Sbjct: 182 QQLTDEELERIEEACDMALELNQSKHRIYEYVESRMSFIAPNLSIIVGASTAAKIMGIAG 241

Query: 243 GLGSLAKMPACNVQLLGAKKKNLAGFSTATSQFRVGYIEQTEIFQSTPPPLKMRACRLVS 302
           GL +L+KMPACNV LLGA++K L+GFS+ +     GYI  ++I QS PP L  +A RLVS
Sbjct: 242 GLTNLSKMPACNVMLLGAQRKTLSGFSSTSVLPHTGYIYHSDIVQSLPPDLHRKAARLVS 301

Query: 303 AKSTLAARVDSTRGDPTGKTGRVFKDEILKKIEKWQEPPPAKQPKPLPVPDSEPKKKRGG 362
           AK TLAARVDS      GK G   K+EI +K +KWQEPPP KQ KPLP P    +KKRGG
Sbjct: 302 AKCTLAARVDSFHESSEGKVGYDLKEEIERKFDKWQEPPPVKQVKPLPAPLDGQRKKRGG 361

Query: 363 RRLRKMKERYATTEMRKLANRMQFGVPEESSLGKAFFQFYNNLDWLNSYSCKTVVIARDG 422
           RR RKMKER   TE+RK ANRM F   EE +                          ++ 
Sbjct: 362 RRYRKMKERLGLTEIRKQANRMSFAEIEEDAY-------------------------QED 421

Query: 423 LGEGYGMLGQAGSGKLR---VSAAQSKLAAKVVKKFKEKR---YGSSGA----TSGLTSS 482
           LG   G LG++GSG++R   V+ A     +K +++  +K+   YG        +SG  SS
Sbjct: 422 LGFSLGHLGKSGSGRIRQAQVNEATKARISKTLQRTLQKQSVVYGGKSTIRDRSSGTASS 481

Query: 483 LAFTPVQGIELSNPQAHLNQLGGGTQSTYFSETGTFSKIR 508
           +AFTP+QG+E+ NPQA   ++    Q  YFS    F K++
Sbjct: 482 VAFTPLQGLEIVNPQAAEKKVAEANQK-YFSSMAEFLKVK 489

[Close]

BLAST of Carg14460 vs. ExPASy TrEMBL
Match: A0A6J1G0W2 (U4/U6 small nuclear ribonucleoprotein Prp31 homolog OS=Cucurbita moschata OX=3662 GN=LOC111449657 PE=3 SV=1)

HSP 1 Score: 897.5 bits (2318), Expect = 2.6e-257
Identity = 484/509 (95.09%), Postives = 484/509 (95.09%), Query Frame = 0


Query: 1   MATLADSFLADLDELSDEDNFPDEEDVDAENMEEDIDGDLADLESLNYDDLDNVSKLQKS 60
           MATLADSFLADLDELSDEDNFPDEEDVDAENMEEDIDGDLADLESLNYDDLDNVSKLQKS
Sbjct: 1   MATLADSFLADLDELSDEDNFPDEEDVDAENMEEDIDGDLADLESLNYDDLDNVSKLQKS 60

Query: 61  QRYSDIMQKVEDALQKDSNISNQGLVLEDDPEYQLIVECNALSVDIENEIIIIHNFIRDK 120
           QRYSDIMQKVEDALQKDSNISNQGLVLEDDPEYQLIVECNALSVDIENEIIIIHNFIRDK
Sbjct: 61  QRYSDIMQKVEDALQKDSNISNQGLVLEDDPEYQLIVECNALSVDIENEIIIIHNFIRDK 120

Query: 121 YRLKFPELESLVHHPIDYARVVKKIGNEMDLTLVDLEGLLPSAVIMVVSVTASTTSGKPL 180
           YRLKFPELESLVHHPIDYARVVKKIGNEMDLTLVDLEGLLPSAVIMVVSVTASTTSGKPL
Sbjct: 121 YRLKFPELESLVHHPIDYARVVKKIGNEMDLTLVDLEGLLPSAVIMVVSVTASTTSGKPL 180

Query: 181 PEEILQKTIDACDRALALDSAKKMVLNFVESRMGYIAPNLSAIVGSAVAAKLMGTAGGLG 240
           PEEILQKTIDACDRALALDSAKKMVLNFVESRMGYIAPNLSAIVGSAVAAKLMGTAGGLG
Sbjct: 181 PEEILQKTIDACDRALALDSAKKMVLNFVESRMGYIAPNLSAIVGSAVAAKLMGTAGGLG 240

Query: 241 SLAKMPACNVQLLGAKKKNLAGFSTATSQFRVGYIEQTEIFQSTPPPLKMRACRLVSAKS 300
           SLAKMPACNVQLLGAKKKNLAGFSTATSQFRVGYIEQTEIFQSTPPPLKMRACRLVSAKS
Sbjct: 241 SLAKMPACNVQLLGAKKKNLAGFSTATSQFRVGYIEQTEIFQSTPPPLKMRACRLVSAKS 300

Query: 301 TLAARVDSTRGDPTGKTGRVFKDEILKKIEKWQEPPPAKQPKPLPVPDSEPKKKRGGRRL 360
           TLAARVDSTRGDPTGKTGRVFKDEILKKIEKWQEPPPAKQPKPLPVPDSEPKKKRGGRRL
Sbjct: 301 TLAARVDSTRGDPTGKTGRVFKDEILKKIEKWQEPPPAKQPKPLPVPDSEPKKKRGGRRL 360

Query: 361 RKMKERYATTEMRKLANRMQFGVPEESSLGKAFFQFYNNLDWLNSYSCKTVVIARDGLGE 420
           RKMKERYATTEMRKLANRMQFGVPEESSLG                         DGLGE
Sbjct: 361 RKMKERYATTEMRKLANRMQFGVPEESSLG-------------------------DGLGE 420

Query: 421 GYGMLGQAGSGKLRVSAAQSKLAAKVVKKFKEKRYGSSGATSGLTSSLAFTPVQGIELSN 480
           GYGMLGQAGSGKLRVSAAQSKLAAKVVKKFKEKRYGSSGATSGLTSSLAFTPVQGIELSN
Sbjct: 421 GYGMLGQAGSGKLRVSAAQSKLAAKVVKKFKEKRYGSSGATSGLTSSLAFTPVQGIELSN 480

Query: 481 PQAHLNQLGGGTQSTYFSETGTFSKIRKN 510
           PQAHLNQLGGGTQSTYFSETGTFSKIRKN
Sbjct: 481 PQAHLNQLGGGTQSTYFSETGTFSKIRKN 484

[Close]

BLAST of Carg14460 vs. ExPASy TrEMBL
Match: A0A6J1HSU1 (U4/U6 small nuclear ribonucleoprotein Prp31 homolog OS=Cucurbita maxima OX=3661 GN=LOC111466452 PE=3 SV=1)

HSP 1 Score: 887.5 bits (2292), Expect = 2.7e-254
Identity = 479/509 (94.11%), Postives = 481/509 (94.50%), Query Frame = 0


Query: 1   MATLADSFLADLDELSDEDNFPDEEDVDAENMEEDIDGDLADLESLNYDDLDNVSKLQKS 60
           MATLADSFLADLDELSDEDNFPDEEDVDAENMEEDIDGDLADLESLNYDDLDNVSKLQKS
Sbjct: 1   MATLADSFLADLDELSDEDNFPDEEDVDAENMEEDIDGDLADLESLNYDDLDNVSKLQKS 60

Query: 61  QRYSDIMQKVEDALQKDSNISNQGLVLEDDPEYQLIVECNALSVDIENEIIIIHNFIRDK 120
           QRYSDIMQKVEDALQKDSNISNQGLVLEDDPEYQLIVECNALSVDIENEIIIIHNFIRDK
Sbjct: 61  QRYSDIMQKVEDALQKDSNISNQGLVLEDDPEYQLIVECNALSVDIENEIIIIHNFIRDK 120

Query: 121 YRLKFPELESLVHHPIDYARVVKKIGNEMDLTLVDLEGLLPSAVIMVVSVTASTTSGKPL 180
           YRLKFPELESLVHHPIDYARVVKKIGNEMDLTLVDLEGLLPSAVIMVVSVTASTTSGKPL
Sbjct: 121 YRLKFPELESLVHHPIDYARVVKKIGNEMDLTLVDLEGLLPSAVIMVVSVTASTTSGKPL 180

Query: 181 PEEILQKTIDACDRALALDSAKKMVLNFVESRMGYIAPNLSAIVGSAVAAKLMGTAGGLG 240
           PEEILQKTIDACDRALALDSAKKMVLNFVESRMGYIAPNLSAIVGSAVAAKLMGTAGGL 
Sbjct: 181 PEEILQKTIDACDRALALDSAKKMVLNFVESRMGYIAPNLSAIVGSAVAAKLMGTAGGLA 240

Query: 241 SLAKMPACNVQLLGAKKKNLAGFSTATSQFRVGYIEQTEIFQSTPPPLKMRACRLVSAKS 300
           SLAKMPACNVQLLGAKKKNLAGFSTATSQFR+GYIEQTEIFQSTPP LKMRACRLVSAKS
Sbjct: 241 SLAKMPACNVQLLGAKKKNLAGFSTATSQFRLGYIEQTEIFQSTPPHLKMRACRLVSAKS 300

Query: 301 TLAARVDSTRGDPTGKTGRVFKDEILKKIEKWQEPPPAKQPKPLPVPDSEPKKKRGGRRL 360
           TLAARVDSTRGDPTGKTGRVFKDEILKKIEKWQEPPPAKQPKPLPVPDSEPKKKRGGRRL
Sbjct: 301 TLAARVDSTRGDPTGKTGRVFKDEILKKIEKWQEPPPAKQPKPLPVPDSEPKKKRGGRRL 360

Query: 361 RKMKERYATTEMRKLANRMQFGVPEESSLGKAFFQFYNNLDWLNSYSCKTVVIARDGLGE 420
           RKMKERYATTEMRKLANRMQFGVPEESSLG                         DGLGE
Sbjct: 361 RKMKERYATTEMRKLANRMQFGVPEESSLG-------------------------DGLGE 420

Query: 421 GYGMLGQAGSGKLRVSAAQSKLAAKVVKKFKEKRYGSSGATSGLTSSLAFTPVQGIELSN 480
           GYGMLGQAGSGKLRVSAAQSKLAAKVVKKFKEKRYGSSGATSGLTSSLAFTPVQGIELSN
Sbjct: 421 GYGMLGQAGSGKLRVSAAQSKLAAKVVKKFKEKRYGSSGATSGLTSSLAFTPVQGIELSN 480

Query: 481 PQAHLNQLGGGTQSTYFSETGTFSKIRKN 510
           PQAH+NQLG GTQSTYFSETGTFSKIRKN
Sbjct: 481 PQAHMNQLGSGTQSTYFSETGTFSKIRKN 484

[Close]

BLAST of Carg14460 vs. ExPASy TrEMBL
Match: A0A6J1DBC9 (U4/U6 small nuclear ribonucleoprotein Prp31 homolog isoform X1 OS=Momordica charantia OX=3673 GN=LOC111019139 PE=3 SV=1)

HSP 1 Score: 875.9 bits (2262), Expect = 8.0e-251
Identity = 472/509 (92.73%), Postives = 479/509 (94.11%), Query Frame = 0


Query: 1   MATLADSFLADLDELSDEDNFPDEEDVDAENMEEDIDGDLADLESLNYDDLDNVSKLQKS 60
           MATLADSFLADLDELSDEDNF DEEDVDAENMEEDIDGDLADLESLNYDDLD+VSKLQK+
Sbjct: 1   MATLADSFLADLDELSDEDNFLDEEDVDAENMEEDIDGDLADLESLNYDDLDSVSKLQKT 60

Query: 61  QRYSDIMQKVEDALQKDSNISNQGLVLEDDPEYQLIVECNALSVDIENEIIIIHNFIRDK 120
           QRY+DIMQKVE ALQKDSNISNQGLVLEDDPEYQLIVECN+LSVDIENEIIIIHNFIRDK
Sbjct: 61  QRYNDIMQKVEGALQKDSNISNQGLVLEDDPEYQLIVECNSLSVDIENEIIIIHNFIRDK 120

Query: 121 YRLKFPELESLVHHPIDYARVVKKIGNEMDLTLVDLEGLLPSAVIMVVSVTASTTSGKPL 180
           YRLKFPELESLVHHPIDYARVVKKIGNEMDLTLVDLEGLLPSAVIMVVSVTASTTSGKPL
Sbjct: 121 YRLKFPELESLVHHPIDYARVVKKIGNEMDLTLVDLEGLLPSAVIMVVSVTASTTSGKPL 180

Query: 181 PEEILQKTIDACDRALALDSAKKMVLNFVESRMGYIAPNLSAIVGSAVAAKLMGTAGGLG 240
           PEEILQKTIDACDRALALDSAKK VLNFVESRMGYIAPNLSAIVGSAVAAKLMGTAGGL 
Sbjct: 181 PEEILQKTIDACDRALALDSAKKKVLNFVESRMGYIAPNLSAIVGSAVAAKLMGTAGGLA 240

Query: 241 SLAKMPACNVQLLGAKKKNLAGFSTATSQFRVGYIEQTEIFQSTPPPLKMRACRLVSAKS 300
           +LAKMPACNVQLLGAKKKNLAGFSTATSQFRVGYIEQTEIFQSTPPPLKMRACRL++AKS
Sbjct: 241 ALAKMPACNVQLLGAKKKNLAGFSTATSQFRVGYIEQTEIFQSTPPPLKMRACRLIAAKS 300

Query: 301 TLAARVDSTRGDPTGKTGRVFKDEILKKIEKWQEPPPAKQPKPLPVPDSEPKKKRGGRRL 360
           TLAARVDSTRGDPTGKTGRVFKDEILKKIEKWQEPPPAKQPKPLPVPDSEPKKKRGGRRL
Sbjct: 301 TLAARVDSTRGDPTGKTGRVFKDEILKKIEKWQEPPPAKQPKPLPVPDSEPKKKRGGRRL 360

Query: 361 RKMKERYATTEMRKLANRMQFGVPEESSLGKAFFQFYNNLDWLNSYSCKTVVIARDGLGE 420
           RKMKERYATTEMRKLANRMQFGVPEESSLG                         DGLGE
Sbjct: 361 RKMKERYATTEMRKLANRMQFGVPEESSLG-------------------------DGLGE 420

Query: 421 GYGMLGQAGSGKLRVSAAQSKLAAKVVKKFKEKRYGSSGATSGLTSSLAFTPVQGIELSN 480
           GYGMLGQAGSGKLRVSAAQSKLAAKVVKKFKEKRYGSSGATSGLTSSLAFTPVQGIELSN
Sbjct: 421 GYGMLGQAGSGKLRVSAAQSKLAAKVVKKFKEKRYGSSGATSGLTSSLAFTPVQGIELSN 480

Query: 481 PQAHLNQLGGGTQSTYFSETGTFSKIRKN 510
           PQAHLNQLG GTQSTYFSETGTFSKIRKN
Sbjct: 481 PQAHLNQLGSGTQSTYFSETGTFSKIRKN 484

[Close]

BLAST of Carg14460 vs. ExPASy TrEMBL
Match: A0A6J1KRI6 (U4/U6 small nuclear ribonucleoprotein Prp31 homolog isoform X1 OS=Cucurbita maxima OX=3661 GN=LOC111497587 PE=3 SV=1)

HSP 1 Score: 872.5 bits (2253), Expect = 8.8e-250
Identity = 469/509 (92.14%), Postives = 477/509 (93.71%), Query Frame = 0


Query: 1   MATLADSFLADLDELSDEDNFPDEEDVDAENMEEDIDGDLADLESLNYDDLDNVSKLQKS 60
           MATLADSFLADLDELSDEDNF DEED DAENMEEDIDGDLADLESLNYDDLD+VSKLQK+
Sbjct: 1   MATLADSFLADLDELSDEDNFLDEEDADAENMEEDIDGDLADLESLNYDDLDSVSKLQKT 60

Query: 61  QRYSDIMQKVEDALQKDSNISNQGLVLEDDPEYQLIVECNALSVDIENEIIIIHNFIRDK 120
           QRY+DIMQKV DAL+ DSN+SNQG VLEDDPEYQLIVECNALSVDIENEIIIIHNFIRDK
Sbjct: 61  QRYNDIMQKVADALEMDSNVSNQGFVLEDDPEYQLIVECNALSVDIENEIIIIHNFIRDK 120

Query: 121 YRLKFPELESLVHHPIDYARVVKKIGNEMDLTLVDLEGLLPSAVIMVVSVTASTTSGKPL 180
           YRLKFPELESLVHHPIDYARVVKKIGNEMDLTLVDLEGLLPSAVIMVVSVTASTTSGKPL
Sbjct: 121 YRLKFPELESLVHHPIDYARVVKKIGNEMDLTLVDLEGLLPSAVIMVVSVTASTTSGKPL 180

Query: 181 PEEILQKTIDACDRALALDSAKKMVLNFVESRMGYIAPNLSAIVGSAVAAKLMGTAGGLG 240
           PEEILQKTIDACDRAL LDSAKKMVLNFVESRMGYIAPNLSAIVGSAVAAKLMGTAGGL 
Sbjct: 181 PEEILQKTIDACDRALTLDSAKKMVLNFVESRMGYIAPNLSAIVGSAVAAKLMGTAGGLV 240

Query: 241 SLAKMPACNVQLLGAKKKNLAGFSTATSQFRVGYIEQTEIFQSTPPPLKMRACRLVSAKS 300
           +LAKMPACNVQLLGAKKKNLAGFSTATSQFRVGYIEQTEIFQSTPPPLKMRACRL++AKS
Sbjct: 241 ALAKMPACNVQLLGAKKKNLAGFSTATSQFRVGYIEQTEIFQSTPPPLKMRACRLIAAKS 300

Query: 301 TLAARVDSTRGDPTGKTGRVFKDEILKKIEKWQEPPPAKQPKPLPVPDSEPKKKRGGRRL 360
           TLAARVDSTRGDPTGKTGRVFKDEILKKIEKWQEPPPAKQPKPLPVPDSEPKKKRGGRRL
Sbjct: 301 TLAARVDSTRGDPTGKTGRVFKDEILKKIEKWQEPPPAKQPKPLPVPDSEPKKKRGGRRL 360

Query: 361 RKMKERYATTEMRKLANRMQFGVPEESSLGKAFFQFYNNLDWLNSYSCKTVVIARDGLGE 420
           RKMKERYATTEMRKLANRMQFGVPEESSLG                         DGLGE
Sbjct: 361 RKMKERYATTEMRKLANRMQFGVPEESSLG-------------------------DGLGE 420

Query: 421 GYGMLGQAGSGKLRVSAAQSKLAAKVVKKFKEKRYGSSGATSGLTSSLAFTPVQGIELSN 480
           GYGMLGQAGSGKLRVSAAQSKLAAKVVKKFKEKRYGSSGATSGLTSSLAFTPVQGIELSN
Sbjct: 421 GYGMLGQAGSGKLRVSAAQSKLAAKVVKKFKEKRYGSSGATSGLTSSLAFTPVQGIELSN 480

Query: 481 PQAHLNQLGGGTQSTYFSETGTFSKIRKN 510
           PQAHLNQLGGGTQSTYFSETGTFSKIRKN
Sbjct: 481 PQAHLNQLGGGTQSTYFSETGTFSKIRKN 484

[Close]

BLAST of Carg14460 vs. ExPASy TrEMBL
Match: A0A6J1GXP7 (U4/U6 small nuclear ribonucleoprotein Prp31 homolog isoform X1 OS=Cucurbita moschata OX=3662 GN=LOC111458320 PE=3 SV=1)

HSP 1 Score: 872.5 bits (2253), Expect = 8.8e-250
Identity = 469/509 (92.14%), Postives = 477/509 (93.71%), Query Frame = 0


Query: 1   MATLADSFLADLDELSDEDNFPDEEDVDAENMEEDIDGDLADLESLNYDDLDNVSKLQKS 60
           MATLADSFLADLDELSDEDNF DEED DAENMEEDIDGDLADLESLNYDDLD+VSKLQK+
Sbjct: 1   MATLADSFLADLDELSDEDNFLDEEDADAENMEEDIDGDLADLESLNYDDLDSVSKLQKT 60

Query: 61  QRYSDIMQKVEDALQKDSNISNQGLVLEDDPEYQLIVECNALSVDIENEIIIIHNFIRDK 120
           QRY+DIMQKV DAL+ DSN+SNQG VLEDDPEYQLIVECNALSVDIENEIIIIHNFIRDK
Sbjct: 61  QRYNDIMQKVADALEMDSNVSNQGFVLEDDPEYQLIVECNALSVDIENEIIIIHNFIRDK 120

Query: 121 YRLKFPELESLVHHPIDYARVVKKIGNEMDLTLVDLEGLLPSAVIMVVSVTASTTSGKPL 180
           YRLKFPELESLVHHPIDYARVVKKIGNEMDLTLVDLEGLLPSAVIMVVSVTASTTSGKPL
Sbjct: 121 YRLKFPELESLVHHPIDYARVVKKIGNEMDLTLVDLEGLLPSAVIMVVSVTASTTSGKPL 180

Query: 181 PEEILQKTIDACDRALALDSAKKMVLNFVESRMGYIAPNLSAIVGSAVAAKLMGTAGGLG 240
           PEEILQKTIDACDRAL LDSAKKMVLNFVESRMGYIAPNLSAIVGSAVAAKLMGTAGGL 
Sbjct: 181 PEEILQKTIDACDRALTLDSAKKMVLNFVESRMGYIAPNLSAIVGSAVAAKLMGTAGGLV 240

Query: 241 SLAKMPACNVQLLGAKKKNLAGFSTATSQFRVGYIEQTEIFQSTPPPLKMRACRLVSAKS 300
           +LAKMPACNVQLLGAKKKNLAGFSTATSQFRVGYIEQTEIFQSTPPPLKMRACRL++AKS
Sbjct: 241 ALAKMPACNVQLLGAKKKNLAGFSTATSQFRVGYIEQTEIFQSTPPPLKMRACRLIAAKS 300

Query: 301 TLAARVDSTRGDPTGKTGRVFKDEILKKIEKWQEPPPAKQPKPLPVPDSEPKKKRGGRRL 360
           TLAARVDSTRGDPTGKTGRVFKDEILKKIEKWQEPPPAKQPKPLPVPDSEPKKKRGGRRL
Sbjct: 301 TLAARVDSTRGDPTGKTGRVFKDEILKKIEKWQEPPPAKQPKPLPVPDSEPKKKRGGRRL 360

Query: 361 RKMKERYATTEMRKLANRMQFGVPEESSLGKAFFQFYNNLDWLNSYSCKTVVIARDGLGE 420
           RKMKERYATTEMRKLANRMQFGVPEESSLG                         DGLGE
Sbjct: 361 RKMKERYATTEMRKLANRMQFGVPEESSLG-------------------------DGLGE 420

Query: 421 GYGMLGQAGSGKLRVSAAQSKLAAKVVKKFKEKRYGSSGATSGLTSSLAFTPVQGIELSN 480
           GYGMLGQAGSGKLRVSAAQSKLAAKVVKKFKEKRYGSSGATSGLTSSLAFTPVQGIELSN
Sbjct: 421 GYGMLGQAGSGKLRVSAAQSKLAAKVVKKFKEKRYGSSGATSGLTSSLAFTPVQGIELSN 480

Query: 481 PQAHLNQLGGGTQSTYFSETGTFSKIRKN 510
           PQAHLNQLGGGTQSTYFSETGTFSKIRKN
Sbjct: 481 PQAHLNQLGGGTQSTYFSETGTFSKIRKN 484

[Close]

BLAST of Carg14460 vs. TAIR 10
Match: AT1G60170.1 (pre-mRNA processing ribonucleoprotein binding region-containing protein )

HSP 1 Score: 681.4 bits (1757), Expect = 5.6e-196
Identity = 374/510 (73.33%), Postives = 419/510 (82.16%), Query Frame = 0


Query: 1   MATLADSFLADLDELSDEDNFPDEEDVDAENMEEDIDGDLADLESLNYDDLDNVSKLQKS 60
           MATL DSFLADLDELSD +   DE D D    EED+D D+ADLE+LNYDDLDNVSKLQKS
Sbjct: 1   MATLEDSFLADLDELSDNEAELDENDGDVGKEEEDVDMDMADLETLNYDDLDNVSKLQKS 60

Query: 61  QRYSDIMQKVEDALQKDSNISNQGLVLEDDPEYQLIVECNALSVDIENEIIIIHNFIRDK 120
           QRY+DIM KVE+AL KDS+ + +G VLEDDPEY+LIV+CN LSVDIENEI+I+HNFI+DK
Sbjct: 61  QRYADIMHKVEEALGKDSDGAEKGTVLEDDPEYKLIVDCNQLSVDIENEIVIVHNFIKDK 120

Query: 121 YRLKFPELESLVHHPIDYARVVKKIGNEMDLTLVDLEGLLPSAVIMVVSVTASTTSGKPL 180
           Y+LKF ELESLVHHPIDYA VVKKIGNE DL LVDL  LLPSA+IMVVSVTA TT G  L
Sbjct: 121 YKLKFQELESLVHHPIDYACVVKKIGNETDLALVDLADLLPSAIIMVVSVTALTTKGSAL 180

Query: 181 PEEILQKTIDACDRALALDSAKKMVLNFVESRMGYIAPNLSAIVGSAVAAKLMGTAGGLG 240
           PE++LQK ++ACDRAL LDSA+K VL FVES+MG IAPNLSAIVGSAVAAKLMGTAGGL 
Sbjct: 181 PEDVLQKVLEACDRALDLDSARKKVLEFVESKMGSIAPNLSAIVGSAVAAKLMGTAGGLS 240

Query: 241 SLAKMPACNVQLLGAKKKNLAGFSTATSQFRVGYIEQTEIFQSTPPPLKMRACRLVSAKS 300
           +LAKMPACNVQ+LG K+KNLAGFS+ATSQ RVGY+EQTEI+QSTPP L+ RA RLV+AKS
Sbjct: 241 ALAKMPACNVQVLGHKRKNLAGFSSATSQSRVGYLEQTEIYQSTPPGLQARAGRLVAAKS 300

Query: 301 TLAARVDSTRGDPTGKTGRVFKDEILKKIEKWQEPPPAKQPKPLPVPDSEPKKKRGGRRL 360
           TLAARVD+TRGDP G +G+ F++EI KKIEKWQEPPPA+QPKPLPVPDSEPKK+RGGRRL
Sbjct: 301 TLAARVDATRGDPLGISGKAFREEIRKKIEKWQEPPPARQPKPLPVPDSEPKKRRGGRRL 360

Query: 361 RKMKERYATTEMRKLANRMQFGVPEESSLGKAFFQFYNNLDWLNSYSCKTVVIARDGLGE 420
           RKMKERY  T+MRKLANRM FG PEESSLG                         DGLGE
Sbjct: 361 RKMKERYQVTDMRKLANRMAFGTPEESSLG-------------------------DGLGE 420

Query: 421 GYGMLGQAGSGKLRVSAAQSKLA--AKVVKKFKEKRYGSSGATSGLTSSLAFTPVQGIEL 480
           GYGMLGQAGS +LRVS+  SKL   AKV KK KE++Y     TSGLTSSLAFTPVQGIEL
Sbjct: 421 GYGMLGQAGSNRLRVSSVPSKLKINAKVAKKLKERQYAGGATTSGLTSSLAFTPVQGIEL 480

Query: 481 SNPQAHLNQLGGGTQSTYFSETGTFSKIRK 509
            NPQ  L  LG GTQSTYFSE+GTFSK++K
Sbjct: 481 CNPQQALG-LGSGTQSTYFSESGTFSKLKK 484

[Close]

BLAST of Carg14460 vs. TAIR 10
Match: AT1G70400.1 (CONTAINS InterPro DOMAIN/s: NOSIC (InterPro:IPR012976); BEST Arabidopsis thaliana protein match is: pre-mRNA processing ribonucleoprotein binding region-containing protein (TAIR:AT1G60170.1); Has 479 Blast hits to 479 proteins in 178 species: Archae - 0; Bacteria - 0; Metazoa - 125; Fungi - 138; Plants - 124; Viruses - 0; Other Eukaryotes - 92 (source: NCBI BLink). )

HSP 1 Score: 212.2 bits (539), Expect = 9.5e-55
Identity = 117/172 (68.02%), Postives = 138/172 (80.23%), Query Frame = 0


Query: 39  DLADLESLNYDDLDNVSKLQKSQRYSDIMQKVEDALQKDSNISNQGLVLEDDPEYQLIVE 98
           D+ +L +L YDDLD+VSKLQKS+RY+DIMQ+VE+AL        +G VLE     +LIV+
Sbjct: 2   DMTELNTLTYDDLDSVSKLQKSRRYADIMQQVEEAL--------EGSVLE---YKKLIVD 61

Query: 99  CNALSVDIENEIIIIHNFIRDKYRLKFPELESLVHHPIDYARVVKKIGNEMDLTLVDLEG 158
           C  L VDIENEI+I+ NFIRDKYR+KF ELE LV HPIDYARVVK+IGNEMDL LVDLEG
Sbjct: 62  CKQLLVDIENEIVIVQNFIRDKYRVKFQELELLVPHPIDYARVVKRIGNEMDLKLVDLEG 121

Query: 159 LLPSAVIMVVSVTASTTSGKPLPEEILQKTIDACDRALALDSAKKMVLNFVE 211
           LLPSA+IMV+ VTA TT G  LPE++L KTIDACDRAL LDSA+K VL FV+
Sbjct: 122 LLPSAMIMVLLVTALTTKGNQLPEDVLLKTIDACDRALDLDSARKKVLEFVD 162

[Close]

BLAST of Carg14460 vs. TAIR 10
Match: AT1G70400.2 (CONTAINS InterPro DOMAIN/s: NOSIC (InterPro:IPR012976); BEST Arabidopsis thaliana protein match is: pre-mRNA processing ribonucleoprotein binding region-containing protein (TAIR:AT1G60170.1); Has 35333 Blast hits to 34131 proteins in 2444 species: Archae - 798; Bacteria - 22429; Metazoa - 974; Fungi - 991; Plants - 531; Viruses - 0; Other Eukaryotes - 9610 (source: NCBI BLink). )

HSP 1 Score: 204.9 bits (520), Expect = 1.5e-52
Identity = 113/165 (68.48%), Postives = 133/165 (80.61%), Query Frame = 0


Query: 39  DLADLESLNYDDLDNVSKLQKSQRYSDIMQKVEDALQKDSNISNQGLVLEDDPEYQLIVE 98
           D+ +L +L YDDLD+VSKLQKS+RY+DIMQ+VE+AL        +G VLE     +LIV+
Sbjct: 2   DMTELNTLTYDDLDSVSKLQKSRRYADIMQQVEEAL--------EGSVLE---YKKLIVD 61

Query: 99  CNALSVDIENEIIIIHNFIRDKYRLKFPELESLVHHPIDYARVVKKIGNEMDLTLVDLEG 158
           C  L VDIENEI+I+ NFIRDKYR+KF ELE LV HPIDYARVVK+IGNEMDL LVDLEG
Sbjct: 62  CKQLLVDIENEIVIVQNFIRDKYRVKFQELELLVPHPIDYARVVKRIGNEMDLKLVDLEG 121

Query: 159 LLPSAVIMVVSVTASTTSGKPLPEEILQKTIDACDRALALDSAKK 204
           LLPSA+IMV+ VTA TT G  LPE++L KTIDACDRAL LDSA+K
Sbjct: 122 LLPSAMIMVLLVTALTTKGNQLPEDVLLKTIDACDRALDLDSARK 155

[Close]

BLAST of Carg14460 vs. TAIR 10
Match: AT1G70400.3 (FUNCTIONS IN: molecular_function unknown; INVOLVED IN: biological_process unknown; LOCATED IN: cellular_component unknown; EXPRESSED IN: egg cell; CONTAINS InterPro DOMAIN/s: NOSIC (InterPro:IPR012976); BEST Arabidopsis thaliana protein match is: pre-mRNA processing ribonucleoprotein binding region-containing protein (TAIR:AT1G60170.1); Has 484 Blast hits to 484 proteins in 190 species: Archae - 0; Bacteria - 0; Metazoa - 149; Fungi - 134; Plants - 124; Viruses - 0; Other Eukaryotes - 77 (source: NCBI BLink). )

HSP 1 Score: 204.9 bits (520), Expect = 1.5e-52
Identity = 113/165 (68.48%), Postives = 133/165 (80.61%), Query Frame = 0


Query: 39  DLADLESLNYDDLDNVSKLQKSQRYSDIMQKVEDALQKDSNISNQGLVLEDDPEYQLIVE 98
           D+ +L +L YDDLD+VSKLQKS+RY+DIMQ+VE+AL        +G VLE     +LIV+
Sbjct: 2   DMTELNTLTYDDLDSVSKLQKSRRYADIMQQVEEAL--------EGSVLE---YKKLIVD 61

Query: 99  CNALSVDIENEIIIIHNFIRDKYRLKFPELESLVHHPIDYARVVKKIGNEMDLTLVDLEG 158
           C  L VDIENEI+I+ NFIRDKYR+KF ELE LV HPIDYARVVK+IGNEMDL LVDLEG
Sbjct: 62  CKQLLVDIENEIVIVQNFIRDKYRVKFQELELLVPHPIDYARVVKRIGNEMDLKLVDLEG 121

Query: 159 LLPSAVIMVVSVTASTTSGKPLPEEILQKTIDACDRALALDSAKK 204
           LLPSA+IMV+ VTA TT G  LPE++L KTIDACDRAL LDSA+K
Sbjct: 122 LLPSAMIMVLLVTALTTKGNQLPEDVLLKTIDACDRALDLDSARK 155

[Close]

BLAST of Carg14460 vs. TAIR 10
Match: AT3G05060.1 (NOP56-like pre RNA processing ribonucleoprotein )

HSP 1 Score: 100.1 bits (248), Expect = 5.3e-21
Identity = 69/239 (28.87%), Postives = 118/239 (49.37%), Query Frame = 0


Query: 79  NISNQGLVLEDDPEYQLIVECNALSVDIENEIIIIHNFIRDKYRLKFPELESLVHHPIDY 138
           +++   L    D    +I++   L  D++ E+      +R+ Y   FPEL  ++   I Y
Sbjct: 144 SLARYKLKFSSDKVDTMIIQAIGLLDDLDKELNTYAMRVREWYGWHFPELAKIISDNILY 203

Query: 139 ARVVKKIGNEMDLTLVDLEGLLPSAVIMVVSVTASTTSGKPLPEEILQKTIDACDRALAL 198
           A+ VK +GN ++   +D   +L   +   +   A  + G  + +  L    + CD+ L+L
Sbjct: 204 AKSVKLMGNRVNAAKLDFSEILADEIEADLKDAAVISMGTEVSDLDLLHIRELCDQVLSL 263

Query: 199 DSAKKMVLNFVESRMGYIAPNLSAIVGSAVAAKLMGTAGGLGSLAKMPACNVQLLGAKKK 258
              +  + ++++SRM  IAPNL+A+VG  V A+L+   G L +L+K P   VQ+LGA+K 
Sbjct: 264 SEYRAQLYDYLKSRMNTIAPNLTALVGELVGARLISHGGSLLNLSKQPGSTVQILGAEKA 323

Query: 259 NLAGFSTATSQFRVGYIEQTEIFQSTPPPLKMRACRLVSAKSTLAARVDSTRGDPTGKT 318
                 T  +  + G I    +     P  K +  R ++AK+ LA RVD+  GD    T
Sbjct: 324 LFRALKTKHATPKYGLIFHASLVGQAAPKHKGKISRSLAAKTVLAIRVDAL-GDSQDNT 381

The following BLAST results are available for this feature:

BLAST of Carg14460 vs. NCBI nr


Match Name	E-value	Identity	Description
KAG7012823.1	2.1e-277	100.00	U4/U6 small nuclear ribonucleoprotein Prp31-like protein [Cucurbita argyrosperma...	[more]
XP_022945419.1	5.3e-257	95.09	U4/U6 small nuclear ribonucleoprotein Prp31 homolog [Cucurbita moschata] >XP_022...	[more]
XP_022966885.1	5.5e-254	94.11	U4/U6 small nuclear ribonucleoprotein Prp31 homolog [Cucurbita maxima]	[more]
XP_022151138.1	1.6e-250	92.73	U4/U6 small nuclear ribonucleoprotein Prp31 homolog isoform X1 [Momordica charan...	[more]
XP_022956658.1	1.8e-249	92.14	U4/U6 small nuclear ribonucleoprotein Prp31 homolog isoform X1 [Cucurbita moscha...	[more]

BLAST of Carg14460 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q8RXN6	7.8e-195	73.33	U4/U6 small nuclear ribonucleoprotein Prp31 homolog OS=Arabidopsis thaliana OX=3...	[more]
Q8CCF0	1.6e-102	44.91	U4/U6 small nuclear ribonucleoprotein Prp31 OS=Mus musculus OX=10090 GN=Prpf31 P...	[more]
Q5U5C5	4.6e-102	45.58	U4/U6 small nuclear ribonucleoprotein Prp31 OS=Xenopus laevis OX=8355 GN=prpf31 ...	[more]
Q8WWY3	1.3e-101	44.91	U4/U6 small nuclear ribonucleoprotein Prp31 OS=Homo sapiens OX=9606 GN=PRPF31 PE...	[more]
Q6NVP6	1.1e-100	45.00	U4/U6 small nuclear ribonucleoprotein Prp31 OS=Xenopus tropicalis OX=8364 GN=prp...	[more]

BLAST of Carg14460 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1G0W2	2.6e-257	95.09	U4/U6 small nuclear ribonucleoprotein Prp31 homolog OS=Cucurbita moschata OX=366...	[more]
A0A6J1HSU1	2.7e-254	94.11	U4/U6 small nuclear ribonucleoprotein Prp31 homolog OS=Cucurbita maxima OX=3661 ...	[more]
A0A6J1DBC9	8.0e-251	92.73	U4/U6 small nuclear ribonucleoprotein Prp31 homolog isoform X1 OS=Momordica char...	[more]
A0A6J1KRI6	8.8e-250	92.14	U4/U6 small nuclear ribonucleoprotein Prp31 homolog isoform X1 OS=Cucurbita maxi...	[more]
A0A6J1GXP7	8.8e-250	92.14	U4/U6 small nuclear ribonucleoprotein Prp31 homolog isoform X1 OS=Cucurbita mosc...	[more]

BLAST of Carg14460 vs. TAIR 10


Match Name	E-value	Identity	Description
AT1G60170.1	5.6e-196	73.33	pre-mRNA processing ribonucleoprotein binding region-containing protein	[more]
AT1G70400.1	9.5e-55	68.02	CONTAINS InterPro DOMAIN/s: NOSIC (InterPro:IPR012976); BEST Arabidopsis thalian...	[more]
AT1G70400.2	1.5e-52	68.48	CONTAINS InterPro DOMAIN/s: NOSIC (InterPro:IPR012976); BEST Arabidopsis thalian...	[more]
AT1G70400.3	1.5e-52	68.48	FUNCTIONS IN: molecular_function unknown; INVOLVED IN: biological_process unknow...	[more]
AT3G05060.1	5.3e-21	28.87	NOP56-like pre RNA processing ribonucleoprotein	[more]

InterPro

Analysis Name: InterPro Annotations of Silver-seed gourd (SMH-JMG-627) v2
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR012976	NOSIC	SMART	SM00931	NOSIC_2	coord: 95..147 e-value: 6.4E-23 score: 92.1
IPR002687	Nop domain	PFAM	PF01798	Nop	coord: 103..331 e-value: 1.5E-72 score: 243.8
IPR002687	Nop domain	PROSITE	PS51358	NOP	coord: 216..334 score: 36.940376
IPR042239	Nop, C-terminal domain	GENE3D	1.10.246.90	Nop domain	coord: 217..342 e-value: 7.9E-40 score: 137.5
IPR019175	Prp31 C-terminal	PFAM	PF09785	Prp31_C	coord: 414..481 e-value: 2.2E-14 score: 54.4 coord: 339..388 e-value: 8.9E-22 score: 78.3
None	No IPR available	GENE3D	1.10.287.4070		coord: 77..216 e-value: 9.3E-57 score: 192.8
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 331..360
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 1..36
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 11..36
None	No IPR available	PANTHER	PTHR13904:SF0	U4/U6 SMALL NUCLEAR RIBONUCLEOPROTEIN PRP31	coord: 1..506
IPR027105	U4/U6 small nuclear ribonucleoprotein Prp31	PANTHER	PTHR13904	PRE-MRNA SPLICING FACTOR PRP31	coord: 1..506
IPR036070	Nop domain superfamily	SUPERFAMILY	89124	Nop domain	coord: 88..334

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Carg14460-RA	Carg14460-RA	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0000244	spliceosomal tri-snRNP complex assembly
biological_process	GO:0000398	mRNA splicing, via spliceosome
cellular_component	GO:0071011	precatalytic spliceosome
cellular_component	GO:0005687	U4 snRNP
cellular_component	GO:0046540	U4/U6 x U5 tri-snRNP complex
molecular_function	GO:0003723	RNA binding