Carg14351 (gene) Silver-seed gourd (SMH-JMG-627) v2

Overview
NameCarg14351
Typegene
OrganismCucurbita argyrosperma subsp. argyrosperma cv. SMH-JMG-627 (Silver-seed gourd (SMH-JMG-627) v2)
DescriptionXyloglucan endotransglucosylase/hydrolase
LocationCarg_Chr04: 10057004 .. 10058166 (-)
RNA-Seq ExpressionCarg14351
SyntenyCarg14351
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideexonCDS
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGAATTGCCCCTCTTAAGCTTCTTCCTTCTGTGCATGTTTTTTGCAGCCCCTTCTTGTGGTACATACTACACACCTCCAAGCGTCCCACGTTTAACAGATCTCGTACCCAATGTCTCAATCGATCAATACTTCGCCAAGATTTTTGGGGCGGCAAATATCCAGCTGATGAGCAATGGGTCCTCTCTCAATCTCACTCTGAACAAACATTCTGGTAAATCATCATTGGATCTTAGCTTCTCCTTTCAGCCCGACTCTGTTTTGATTCATAACTTCTTCTCTTTTCATTTCTGGAGGTGCTGGTTTGGTCTCGAGGAGCAAATATCATTATGGGTTCTTCAGTGCTTCAATTAAGCTTCCATCAGGCTTCACTTCAGGGGTTGTAGTGGCTTTTTATGTAAGATTTTCCCCCATTTCTTATTATCCTCTGTTTTGCTTACCAGTTTGGTCCACAACCAACGAGATGATGAGGGCCATGTTCAGCTGTCGAATGCAGATGTTTATACCCATTCTCATGACGAGATTGACATAGAGCTGCTGGGACATGACAAGAGAAAAGATTGGGTTATTCAAACAAATATGTATGCAAATGGAAGCGTCAAGACAGGGAGAGAAGAGAAATTCTACCTCTGGTTTGATCCCAGCGTGAAGTACCACGATTACACCATCATTTGGAACAAATATCATACAGTGTAAGTGCATCAAAAGGCAGGATCATTTTTGTTGGTCGTTGTACTTCTCTGTTCTTCTAAAAAGTGCATCGGAATTCAAGCAGGTTTTTGGTGGATAATGTTCCAATCAGAGAGCTGAGAAATTCTGAAGCTTTCTATCCATCAAAACCCATGTCAGTTTTCGTCACGATATGGGATGGTTCAGAATGGGCAACTCATGGAGGAAAGCACCCAGTAGACTATAGGTATGCACCATATGTGGCTTCTTTTGAAGAAATGGAGATCAATGGAAGTGTATCACCACCAATAGCCACTGTTCCTTCAAGCTCCAAAACCAATGTCTCGAGCCCGGATACCGTGGAGGGTCCAGAATTTATAAAGCTATCACAGCAGCAAATAGATGCCATGGATTGGGCAAGGAGGAAGCTCATGTTCTACTCCTATTGTAAAGATACATCCAGATACAAAGTTTTGCCACCAGAGTGCAAATAG

mRNA sequence

ATGGAATTGCCCCTCTTAAGCTTCTTCCTTCTGTGCATGTTTTTTGCAGCCCCTTCTTGTGGTACATACTACACACCTCCAAGCGTCCCACGTTTAACAGATCTCGTACCCAATGTCTCAATCGATCAATACTTCGCCAAGATTTTTGGGGCGGCAAATATCCAGCTGATGAGCAATGGGTCCTCTCTCAATCTCACTCTGAACAAACATTCTGGTAAATCATCATTGGATCTTAGCTTCTCCTTTCAGCCCGACTCTGTTTTGATTCATAACTTCTTCTCTTTTCATTTCTGGAGGTGCTGGTTTGGTCTCGAGGAGCAAATATCATTATGGGTTCTTCATTTGGTCCACAACCAACGAGATGATGAGGGCCATGTTCAGCTGTCGAATGCAGATGTTTATACCCATTCTCATGACGAGATTGACATAGAGCTGCTGGGACATGACAAGAGAAAAGATTGGGTTATTCAAACAAATATGTATGCAAATGGAAGCGTCAAGACAGGGAGAGAAGAGAAATTCTACCTCTGGTTTGATCCCAGCGTGAAGTACCACGATTACACCATCATTTGGAACAAATATCATACAGTGTTTTTGGTGGATAATGTTCCAATCAGAGAGCTGAGAAATTCTGAAGCTTTCTATCCATCAAAACCCATGTCAGTTTTCGTCACGATATGGGATGGTTCAGAATGGGCAACTCATGGAGGAAAGCACCCAGTAGACTATAGGTATGCACCATATGTGGCTTCTTTTGAAGAAATGGAGATCAATGGAAGTGTATCACCACCAATAGCCACTGTTCCTTCAAGCTCCAAAACCAATGTCTCGAGCCCGGATACCGTGGAGGGTCCAGAATTTATAAAGCTATCACAGCAGCAAATAGATGCCATGGATTGGGCAAGGAGGAAGCTCATGTTCTACTCCTATTGTAAAGATACATCCAGATACAAAGTTTTGCCACCAGAGTGCAAATAG

Coding sequence (CDS)

ATGGAATTGCCCCTCTTAAGCTTCTTCCTTCTGTGCATGTTTTTTGCAGCCCCTTCTTGTGGTACATACTACACACCTCCAAGCGTCCCACGTTTAACAGATCTCGTACCCAATGTCTCAATCGATCAATACTTCGCCAAGATTTTTGGGGCGGCAAATATCCAGCTGATGAGCAATGGGTCCTCTCTCAATCTCACTCTGAACAAACATTCTGGTAAATCATCATTGGATCTTAGCTTCTCCTTTCAGCCCGACTCTGTTTTGATTCATAACTTCTTCTCTTTTCATTTCTGGAGGTGCTGGTTTGGTCTCGAGGAGCAAATATCATTATGGGTTCTTCATTTGGTCCACAACCAACGAGATGATGAGGGCCATGTTCAGCTGTCGAATGCAGATGTTTATACCCATTCTCATGACGAGATTGACATAGAGCTGCTGGGACATGACAAGAGAAAAGATTGGGTTATTCAAACAAATATGTATGCAAATGGAAGCGTCAAGACAGGGAGAGAAGAGAAATTCTACCTCTGGTTTGATCCCAGCGTGAAGTACCACGATTACACCATCATTTGGAACAAATATCATACAGTGTTTTTGGTGGATAATGTTCCAATCAGAGAGCTGAGAAATTCTGAAGCTTTCTATCCATCAAAACCCATGTCAGTTTTCGTCACGATATGGGATGGTTCAGAATGGGCAACTCATGGAGGAAAGCACCCAGTAGACTATAGGTATGCACCATATGTGGCTTCTTTTGAAGAAATGGAGATCAATGGAAGTGTATCACCACCAATAGCCACTGTTCCTTCAAGCTCCAAAACCAATGTCTCGAGCCCGGATACCGTGGAGGGTCCAGAATTTATAAAGCTATCACAGCAGCAAATAGATGCCATGGATTGGGCAAGGAGGAAGCTCATGTTCTACTCCTATTGTAAAGATACATCCAGATACAAAGTTTTGCCACCAGAGTGCAAATAG

Protein sequence

MELPLLSFFLLCMFFAAPSCGTYYTPPSVPRLTDLVPNVSIDQYFAKIFGAANIQLMSNGSSLNLTLNKHSGKSSLDLSFSFQPDSVLIHNFFSFHFWRCWFGLEEQISLWVLHLVHNQRDDEGHVQLSNADVYTHSHDEIDIELLGHDKRKDWVIQTNMYANGSVKTGREEKFYLWFDPSVKYHDYTIIWNKYHTVFLVDNVPIRELRNSEAFYPSKPMSVFVTIWDGSEWATHGGKHPVDYRYAPYVASFEEMEINGSVSPPIATVPSSSKTNVSSPDTVEGPEFIKLSQQQIDAMDWARRKLMFYSYCKDTSRYKVLPPECK
Homology
BLAST of Carg14351 vs. NCBI nr
Match: KAG7032271.1 (putative xyloglucan endotransglucosylase/hydrolase protein 33, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 681.8 bits (1758), Expect = 2.9e-192
Identity = 325/325 (100.00%), Postives = 325/325 (100.00%), Query Frame = 0

Query: 1   MELPLLSFFLLCMFFAAPSCGTYYTPPSVPRLTDLVPNVSIDQYFAKIFGAANIQLMSNG 60
           MELPLLSFFLLCMFFAAPSCGTYYTPPSVPRLTDLVPNVSIDQYFAKIFGAANIQLMSNG
Sbjct: 1   MELPLLSFFLLCMFFAAPSCGTYYTPPSVPRLTDLVPNVSIDQYFAKIFGAANIQLMSNG 60

Query: 61  SSLNLTLNKHSGKSSLDLSFSFQPDSVLIHNFFSFHFWRCWFGLEEQISLWVLHLVHNQR 120
           SSLNLTLNKHSGKSSLDLSFSFQPDSVLIHNFFSFHFWRCWFGLEEQISLWVLHLVHNQR
Sbjct: 61  SSLNLTLNKHSGKSSLDLSFSFQPDSVLIHNFFSFHFWRCWFGLEEQISLWVLHLVHNQR 120

Query: 121 DDEGHVQLSNADVYTHSHDEIDIELLGHDKRKDWVIQTNMYANGSVKTGREEKFYLWFDP 180
           DDEGHVQLSNADVYTHSHDEIDIELLGHDKRKDWVIQTNMYANGSVKTGREEKFYLWFDP
Sbjct: 121 DDEGHVQLSNADVYTHSHDEIDIELLGHDKRKDWVIQTNMYANGSVKTGREEKFYLWFDP 180

Query: 181 SVKYHDYTIIWNKYHTVFLVDNVPIRELRNSEAFYPSKPMSVFVTIWDGSEWATHGGKHP 240
           SVKYHDYTIIWNKYHTVFLVDNVPIRELRNSEAFYPSKPMSVFVTIWDGSEWATHGGKHP
Sbjct: 181 SVKYHDYTIIWNKYHTVFLVDNVPIRELRNSEAFYPSKPMSVFVTIWDGSEWATHGGKHP 240

Query: 241 VDYRYAPYVASFEEMEINGSVSPPIATVPSSSKTNVSSPDTVEGPEFIKLSQQQIDAMDW 300
           VDYRYAPYVASFEEMEINGSVSPPIATVPSSSKTNVSSPDTVEGPEFIKLSQQQIDAMDW
Sbjct: 241 VDYRYAPYVASFEEMEINGSVSPPIATVPSSSKTNVSSPDTVEGPEFIKLSQQQIDAMDW 300

Query: 301 ARRKLMFYSYCKDTSRYKVLPPECK 326
           ARRKLMFYSYCKDTSRYKVLPPECK
Sbjct: 301 ARRKLMFYSYCKDTSRYKVLPPECK 325

BLAST of Carg14351 vs. NCBI nr
Match: KAG6601490.1 (putative xyloglucan endotransglucosylase/hydrolase protein 33, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 577.8 bits (1488), Expect = 5.9e-161
Identity = 283/310 (91.29%), Postives = 285/310 (91.94%), Query Frame = 0

Query: 16  AAPSCGTYYTPPSVPRLTDLVPNVSIDQYFAKIFGAANIQLMSNGSSLNLTLNKHSGKSS 75
           +APSCGTYYTPPSVPRLTDLVPNVSIDQYFAKIFGAANIQLMSNGSSLNLTLNKHSGKSS
Sbjct: 14  SAPSCGTYYTPPSVPRLTDLVPNVSIDQYFAKIFGAANIQLMSNGSSLNLTLNKHSGKSS 73

Query: 76  LDLSFSFQPDSVLIHNFFSFHFWRCWFGLEEQISLWVLHLVHNQRDDEGHVQLSNADVYT 135
           LDLSFSFQPDSVLIHNFFSFHFW                LVHNQRDDEGHVQLSNADVYT
Sbjct: 74  LDLSFSFQPDSVLIHNFFSFHFW---------------SLVHNQRDDEGHVQLSNADVYT 133

Query: 136 HSHDEIDIELLGHDKRKDWVIQTNMYANGSVKTGREEKFYLWFDPSVKYHDYTIIWNKYH 195
           HSHDEIDIELLGHDKRKDWVIQTNMYANGSVKTGREEKFYLWFDPSVKYHDYTIIWNKYH
Sbjct: 134 HSHDEIDIELLGHDKRKDWVIQTNMYANGSVKTGREEKFYLWFDPSVKYHDYTIIWNKYH 193

Query: 196 TVFLVDNVPIRELRNSEAFYPSKPMSVFVTIWDGSEWATHGGKHPVDYRYAPYVASFEEM 255
           TV         ELRNSEAFYPSKPMSVFVTIWDGSEWATHGG+HPVDYRYAPYVASFEEM
Sbjct: 194 TV---------ELRNSEAFYPSKPMSVFVTIWDGSEWATHGGRHPVDYRYAPYVASFEEM 253

Query: 256 EINGSVSPPIATVPSSSKTNVSSPDTVEGPEFIKLSQQQIDAMDWARRKLMFYSYCKDTS 315
           EINGSVSPPIATVPSSSKTNVSSPDTVEGPEFIKLSQQQIDAMDWARRKLMFYSYCKDTS
Sbjct: 254 EINGSVSPPIATVPSSSKTNVSSPDTVEGPEFIKLSQQQIDAMDWARRKLMFYSYCKDTS 299

Query: 316 RYKVLPPECK 326
           RYKVLPPECK
Sbjct: 314 RYKVLPPECK 299

BLAST of Carg14351 vs. NCBI nr
Match: XP_022957166.1 (probable xyloglucan endotransglucosylase/hydrolase protein 33 [Cucurbita moschata])

HSP 1 Score: 542.7 bits (1397), Expect = 2.1e-150
Identity = 270/325 (83.08%), Postives = 281/325 (86.46%), Query Frame = 0

Query: 1   MELPLLSFFLLCMFFAAPSCGTYYTPPSVPRLTDLVPNVSIDQYFAKIFGAANIQLMSNG 60
           MELPLLSFFLLCMFFAAPSCGTYYTPPSVPRLTDLVPNVSIDQYFAKIFGAANIQLMSNG
Sbjct: 1   MELPLLSFFLLCMFFAAPSCGTYYTPPSVPRLTDLVPNVSIDQYFAKIFGAANIQLMSNG 60

Query: 61  SSLNLTLNKHSGKSSLDLSFSFQPDSVLIHNFFSFHFWRCWFGLEEQISLWVLHLVHNQR 120
           SSLNLTLNKHSG              ++  + + + F+     L    +  V+   +   
Sbjct: 61  SSLNLTLNKHSGA------------GLVSRSKYHYGFFSASIKLPSGFTSGVVVAFY--- 120

Query: 121 DDEGHVQLSNADVYTHSHDEIDIELLGHDKRKDWVIQTNMYANGSVKTGREEKFYLWFDP 180
                  LSNADVYTHSHDEIDIELLGHD RKDWVIQTNMYANGSVKTGREEKFYLWFDP
Sbjct: 121 -------LSNADVYTHSHDEIDIELLGHDNRKDWVIQTNMYANGSVKTGREEKFYLWFDP 180

Query: 181 SVKYHDYTIIWNKYHTVFLVDNVPIRELRNSEAFYPSKPMSVFVTIWDGSEWATHGGKHP 240
           SVKYHDYTIIWNKYHTVFLVDNVPIRELRNSEAFYPSKPMSVFVTIWDGSEWATHGGK+P
Sbjct: 181 SVKYHDYTIIWNKYHTVFLVDNVPIRELRNSEAFYPSKPMSVFVTIWDGSEWATHGGKYP 240

Query: 241 VDYRYAPYVASFEEMEINGSVSPPIATVPSSSKTNVSSPDTVEGPEFIKLSQQQIDAMDW 300
           VDYRYAPYVASFEEMEINGSVSPPIATVPSSSKTNVSSPDTVEGPEF+KLSQQQIDAMDW
Sbjct: 241 VDYRYAPYVASFEEMEINGSVSPPIATVPSSSKTNVSSPDTVEGPEFVKLSQQQIDAMDW 300

Query: 301 ARRKLMFYSYCKDTSRYKVLPPECK 326
           ARRKLMFYSYCKDTSRYKVLPPECK
Sbjct: 301 ARRKLMFYSYCKDTSRYKVLPPECK 303

BLAST of Carg14351 vs. NCBI nr
Match: XP_023512499.1 (probable xyloglucan endotransglucosylase/hydrolase protein 33 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 537.0 bits (1382), Expect = 1.2e-148
Identity = 267/325 (82.15%), Postives = 281/325 (86.46%), Query Frame = 0

Query: 1   MELPLLSFFLLCMFFAAPSCGTYYTPPSVPRLTDLVPNVSIDQYFAKIFGAANIQLMSNG 60
           MELPLLSFF+LCMFFAAPSCGTYYTPPSVPRLTDLVPNVSIDQ FAKIFGAANIQLMSNG
Sbjct: 1   MELPLLSFFILCMFFAAPSCGTYYTPPSVPRLTDLVPNVSIDQCFAKIFGAANIQLMSNG 60

Query: 61  SSLNLTLNKHSGKSSLDLSFSFQPDSVLIHNFFSFHFWRCWFGLEEQISLWVLHLVHNQR 120
           SSLNLTLNKHSG              ++  + + + F+     L    +  V+   +   
Sbjct: 61  SSLNLTLNKHSGA------------GLVSRSKYHYGFFSASIKLPSGFTSGVVVAFY--- 120

Query: 121 DDEGHVQLSNADVYTHSHDEIDIELLGHDKRKDWVIQTNMYANGSVKTGREEKFYLWFDP 180
                  LSNADVYTHSHDEIDIELLGHDKRKDWVIQTNMYANGSVKTGREEKFYLWFDP
Sbjct: 121 -------LSNADVYTHSHDEIDIELLGHDKRKDWVIQTNMYANGSVKTGREEKFYLWFDP 180

Query: 181 SVKYHDYTIIWNKYHTVFLVDNVPIRELRNSEAFYPSKPMSVFVTIWDGSEWATHGGKHP 240
           SVKYHDYTIIWNKYHTVFLVDNVPIRELRNSEAFYPSKPMSVFVTIWDGSEWATHGGK+P
Sbjct: 181 SVKYHDYTIIWNKYHTVFLVDNVPIRELRNSEAFYPSKPMSVFVTIWDGSEWATHGGKYP 240

Query: 241 VDYRYAPYVASFEEMEINGSVSPPIATVPSSSKTNVSSPDTVEGPEFIKLSQQQIDAMDW 300
           VDY+YAPYVASFEEMEINGSVSPPIATVPSSSKTNVSSPDTVEGPEFIKLSQ+QIDAMDW
Sbjct: 241 VDYKYAPYVASFEEMEINGSVSPPIATVPSSSKTNVSSPDTVEGPEFIKLSQEQIDAMDW 300

Query: 301 ARRKLMFYSYCKDTSRYKVLPPECK 326
           ARRKLMFYSYC+DTSRYKVLPPECK
Sbjct: 301 ARRKLMFYSYCEDTSRYKVLPPECK 303

BLAST of Carg14351 vs. NCBI nr
Match: XP_022985837.1 (probable xyloglucan endotransglucosylase/hydrolase protein 33 isoform X2 [Cucurbita maxima])

HSP 1 Score: 534.6 bits (1376), Expect = 5.8e-148
Identity = 269/327 (82.26%), Postives = 280/327 (85.63%), Query Frame = 0

Query: 1   MELPL--LSFFLLCMFFAAPSCGTYYTPPSVPRLTDLVPNVSIDQYFAKIFGAANIQLMS 60
           MELPL  LSFFLLCMFFAAPSCGTYYTPPSVPRLTDLVPNVSIDQYFAKIFG ANIQLMS
Sbjct: 1   MELPLLSLSFFLLCMFFAAPSCGTYYTPPSVPRLTDLVPNVSIDQYFAKIFGGANIQLMS 60

Query: 61  NGSSLNLTLNKHSGKSSLDLSFSFQPDSVLIHNFFSFHFWRCWFGLEEQISLWVLHLVHN 120
           NGSSLNLTLNK SG              ++  N + + F+     L    +  V+   + 
Sbjct: 61  NGSSLNLTLNKLSGA------------GLVSRNKYHYGFFSASIKLPSGFTSGVVVAFY- 120

Query: 121 QRDDEGHVQLSNADVYTHSHDEIDIELLGHDKRKDWVIQTNMYANGSVKTGREEKFYLWF 180
                    LSNADVYTHSHDEIDIELLGHDKRKDWVIQTNMYANGSVKTGREEKFYLWF
Sbjct: 121 ---------LSNADVYTHSHDEIDIELLGHDKRKDWVIQTNMYANGSVKTGREEKFYLWF 180

Query: 181 DPSVKYHDYTIIWNKYHTVFLVDNVPIRELRNSEAFYPSKPMSVFVTIWDGSEWATHGGK 240
           DPSVKYHDYTIIWNKYHTVFLVDNVPIRELRNSEAFYPSKPMSVFVTIWDGSEWATHGGK
Sbjct: 181 DPSVKYHDYTIIWNKYHTVFLVDNVPIRELRNSEAFYPSKPMSVFVTIWDGSEWATHGGK 240

Query: 241 HPVDYRYAPYVASFEEMEINGSVSPPIATVPSSSKTNVSSPDTVEGPEFIKLSQQQIDAM 300
           +PVDY+YAPYVASFEEMEINGS+SPPIATVPSSSKTNVSSPDTVEGPEFIKLSQQQIDAM
Sbjct: 241 YPVDYKYAPYVASFEEMEINGSISPPIATVPSSSKTNVSSPDTVEGPEFIKLSQQQIDAM 300

Query: 301 DWARRKLMFYSYCKDTSRYKVLPPECK 326
           DWARRKLMFYSYCKDTSRYKVLPPECK
Sbjct: 301 DWARRKLMFYSYCKDTSRYKVLPPECK 305

BLAST of Carg14351 vs. ExPASy Swiss-Prot
Match: Q8LC45 (Probable xyloglucan endotransglucosylase/hydrolase protein 33 OS=Arabidopsis thaliana OX=3702 GN=XTH33 PE=1 SV=2)

HSP 1 Score: 299.7 bits (766), Expect = 4.1e-80
Identity = 159/323 (49.23%), Postives = 208/323 (64.40%), Query Frame = 0

Query: 9   FLLCMFFAAPSCGTYYTPPSVPRLTDLVPNVSIDQYFAKIFGAANIQLMSNGSSLNLTLN 68
           F LC      S    +T P+V RLTD    ++I+  F++ FGA NIQ+  NGS   LTL+
Sbjct: 11  FCLCSLSLVSSHSRKFTTPNVTRLTDQFSKIAIENGFSRRFGAHNIQV--NGSLAKLTLD 70

Query: 69  KHSGKSSLDLSFSFQPDSVLIHNFFSFHFWRCWFGLEEQISLWVLHLVHNQRDDEGHVQL 128
           K SG              ++  N + + F+     L    +  V+   +          L
Sbjct: 71  KSSGA------------GLVSKNKYHYGFFSARLKLPAGFASGVVVAFY----------L 130

Query: 129 SNADVYTHSHDEIDIELLGHDKRKDWVIQTNMYANGSVKTGREEKFYLWFDPSVKYHDYT 188
           SNA+ Y  SHDEIDIELLG  +R DW IQTN+YANGS +TGREEKFY WFDP+  +HDYT
Sbjct: 131 SNAETYPKSHDEIDIELLGRSRRDDWTIQTNVYANGSTRTGREEKFYFWFDPTQAFHDYT 190

Query: 189 IIWNKYHTVFLVDNVPIRELRNSEAF---YPSKPMSVFVTIWDGSEWATHGGKHPVDYRY 248
           +IWN +HTVFLVDN+P+R+  N  AF   YPSKPMS++VT+WDGSEWAT GGK+PV+Y+Y
Sbjct: 191 LIWNSHHTVFLVDNIPVRQFPNRGAFTSAYPSKPMSLYVTVWDGSEWATKGGKYPVNYKY 250

Query: 249 APYVASFEEMEING-SVSPPIATVP---SSSKTNVSSPDTVEGPEFIKLSQQQIDAMDWA 308
           AP+V S  ++E++G SV+   +T     + S  ++SS D V+G +F  LS+ QI+AMDWA
Sbjct: 251 APFVVSVADVELSGCSVNNGSSTGSGPCTKSGGSISSLDPVDGQDFATLSKNQINAMDWA 309

Query: 309 RRKLMFYSYCKDTSRYKVLPPEC 325
           RRKLMFYSYC D  RYKV+P EC
Sbjct: 311 RRKLMFYSYCSDKPRYKVMPAEC 309

BLAST of Carg14351 vs. ExPASy Swiss-Prot
Match: Q8LDS2 (Probable xyloglucan endotransglucosylase/hydrolase protein 27 OS=Arabidopsis thaliana OX=3702 GN=XTH27 PE=2 SV=2)

HSP 1 Score: 214.9 bits (546), Expect = 1.3e-54
Identity = 111/292 (38.01%), Postives = 171/292 (58.56%), Query Frame = 0

Query: 36  VPNVSIDQYFAKIFGAANIQLMSNGSSLNLTLNKHSGKSSLDLSFSFQPDSVLIHNFFSF 95
           +P  S ++ + ++FG  N+ +  +G S+ LTL++ +G         F  +   +H FFS 
Sbjct: 26  LPITSFEESYTQLFGDKNLFVHQDGKSVRLTLDERTGS-------GFVSNDYYLHGFFS- 85

Query: 96  HFWRCWFGLEEQISLWVLHLVHNQRDDEGHVQLSNADVYTHSHDEIDIELLGHDKRKDWV 155
                   L    +  V+   +          +SN D+Y  +HDEID E LG+ + K+W 
Sbjct: 86  ----ASIKLPSDYTAGVVVAFY----------MSNGDMYEKNHDEIDFEFLGNIREKEWR 145

Query: 156 IQTNMYANGSVKTGREEKFYLWFDPSVKYHDYTIIWNKYHTVFLVDNVPIRELRNSE--- 215
           +QTN+Y NGS  +GREE++ LWFDP+  +H Y+I+W+  H +F VDNVPIRE++ +    
Sbjct: 146 VQTNIYGNGSTHSGREERYNLWFDPTEDFHQYSILWSDSHIIFFVDNVPIREVKRTAEMG 205

Query: 216 AFYPSKPMSVFVTIWDGSEWATHGGKHPVDYRYAPYVASFEEMEINGSVSPPIATVPSSS 275
             +PSKPMS++ TIWDGS+WAT+GGK+ V+Y+YAPY+A F ++ ++G    PI   P   
Sbjct: 206 GHFPSKPMSLYTTIWDGSKWATNGGKYGVNYKYAPYIARFSDLVLHGCPVDPIEQFP--- 265

Query: 276 KTNVSSPDTVEGPEFIKLSQQQIDAMDWARRKLMFYSYCKDTSRYKVLPPEC 325
           + +  + + +   + I  SQ+    MD  RR+LM YSYC D +RY V   EC
Sbjct: 266 RCDEGAAEDMRAAQEITPSQR--SKMDVFRRRLMTYSYCYDRARYNVALSEC 290

BLAST of Carg14351 vs. ExPASy Swiss-Prot
Match: Q38909 (Probable xyloglucan endotransglucosylase/hydrolase protein 28 OS=Arabidopsis thaliana OX=3702 GN=XTH28 PE=2 SV=1)

HSP 1 Score: 210.3 bits (534), Expect = 3.3e-53
Identity = 110/292 (37.67%), Postives = 169/292 (57.88%), Query Frame = 0

Query: 36  VPNVSIDQYFAKIFGAANIQLMSNGSSLNLTLNKHSGKSSLDLSFSFQPDSVLIHNFFSF 95
           +P +  D+ + ++FG  N+ +  +G S+ LTL++ +G         F  + + +H FFS 
Sbjct: 26  LPLIQFDEGYTQLFGDQNLIVHRDGKSVRLTLDERTGS-------GFVSNDIYLHGFFSS 85

Query: 96  HFWRCWFGLEEQISLWVLHLVHNQRDDEGHVQLSNADVYTHSHDEIDIELLGHDKRKDWV 155
                   L    S  V+   +          LSN D+Y  +HDEID E LG+ + ++W 
Sbjct: 86  S-----IKLPADYSAGVVIAFY----------LSNGDLYEKNHDEIDFEFLGNIRGREWR 145

Query: 156 IQTNMYANGSVKTGREEKFYLWFDPSVKYHDYTIIWNKYHTVFLVDNVPIRELRNSEAF- 215
           IQTN+Y NGS   GREE++ LWFDP+  +H Y+I+W+  H +F VDNVPIRE++ + +  
Sbjct: 146 IQTNIYGNGSTHLGREERYNLWFDPTEDFHQYSILWSLSHIIFYVDNVPIREVKRTASMG 205

Query: 216 --YPSKPMSVFVTIWDGSEWATHGGKHPVDYRYAPYVASFEEMEINGSVSPPIATVPSSS 275
             +P+KPMS++ TIWDGS+WAT GGK+ V+Y+YAPYV+ F ++ ++G    P    PS  
Sbjct: 206 GDFPAKPMSLYSTIWDGSKWATDGGKYGVNYKYAPYVSQFTDLILHGCAVDPTEKFPSCK 265

Query: 276 KTNVSSPDTVEGPEFIKLSQQQIDAMDWARRKLMFYSYCKDTSRYKVLPPEC 325
              V +          ++++ Q + M+  R+K M YSYC D  RYKV+  EC
Sbjct: 266 DEAVQNLRLAS-----EITESQRNKMEIFRQKHMTYSYCYDHMRYKVVLSEC 290

BLAST of Carg14351 vs. ExPASy Swiss-Prot
Match: Q38908 (Probable xyloglucan endotransglucosylase/hydrolase protein 30 OS=Arabidopsis thaliana OX=3702 GN=XTH30 PE=2 SV=2)

HSP 1 Score: 200.3 bits (508), Expect = 3.4e-50
Identity = 104/289 (35.99%), Postives = 161/289 (55.71%), Query Frame = 0

Query: 39  VSIDQYFAKIFGAANIQLMSNGSSLNLTLNKHSGKSSLDLSFSFQPDSVLIHNFFSFHFW 98
           +S ++  + +FG AN+    +  S+ L L++++G   +              N +   F+
Sbjct: 30  LSFEESLSPLFGDANLVRSPDDLSVRLLLDRYTGSGFIS------------SNMYQHGFY 89

Query: 99  RCWFGLEEQISLWVLHLVHNQRDDEGHVQLSNADVYTHSHDEIDIELLGHDKRKDWVIQT 158
                L    +  V+   +           SN DV+  +HDE+DIE LG+ K K W  QT
Sbjct: 90  SSMIKLPADYTAGVVVAFYT----------SNGDVFEKTHDELDIEFLGNIKGKPWRFQT 149

Query: 159 NMYANGSVKTGREEKFYLWFDPSVKYHDYTIIWNKYHTVFLVDNVPIRELRNSEAF---Y 218
           N+Y NGS   GREE++ LWFDPS ++H Y+I+W  +  +F VD+VPIRE+  ++A    Y
Sbjct: 150 NLYGNGSTHRGREERYRLWFDPSKEFHRYSILWTPHKIIFWVDDVPIREVIRNDAMGADY 209

Query: 219 PSKPMSVFVTIWDGSEWATHGGKHPVDYRYAPYVASFEEMEINGSVSPPIATVPSSSKTN 278
           P+KPM+++ TIWD S+WAT GGK+  +Y++AP+VA F+   ++G    PI  VP     +
Sbjct: 210 PAKPMALYATIWDASDWATSGGKYKANYKFAPFVAEFKSFSLDGCSVDPIQEVPMDCSDS 269

Query: 279 VSSPDTVEGPEFIKLSQQQIDAMDWARRKLMFYSYCKDTSRYKVLPPEC 325
           V   D +E  ++  ++  Q  AM   R++ M+YSYC DT RY    PEC
Sbjct: 270 V---DFLESQDYSSINSHQRAAMRRFRQRFMYYSYCYDTLRYPEPLPEC 293

BLAST of Carg14351 vs. ExPASy Swiss-Prot
Match: Q8L7H3 (Probable xyloglucan endotransglucosylase/hydrolase protein 29 OS=Arabidopsis thaliana OX=3702 GN=XTH29 PE=2 SV=1)

HSP 1 Score: 191.8 bits (486), Expect = 1.2e-47
Identity = 110/297 (37.04%), Postives = 159/297 (53.54%), Query Frame = 0

Query: 39  VSIDQYFAKIFGAANIQLMSNGSSLNLTLNKHSGKSSLDLSFSFQPDSVLIHNFFSFHFW 98
           +  D+  + +FG  N+    +  S+ L L+K++G         F   S+  H FFS    
Sbjct: 38  IFFDEGLSHLFGEGNLIRSPDDRSVRLLLDKYTGS-------GFISSSMYQHGFFS---- 97

Query: 99  RCWFGLEEQISLWVLHLVHNQRDDEGHVQLSNADVYTHSHDEIDIELLGHDKRKDWVIQT 158
                L +    +   +V            SN DV+   HDE+DIE LG+ + K W  QT
Sbjct: 98  ----SLIKLPGAYTAGIV-------VAFYTSNGDVFVKDHDELDIEFLGNLEGKPWRFQT 157

Query: 159 NMYANGSVKTGREEKFYLWFDPSVKYHDYTIIWNKYHTVFLVDNVPIRELRNSEAF---Y 218
           NMY NGS   GREE++ LWFDPS ++H Y+I+W     +F VD+VPIRE+   E     Y
Sbjct: 158 NMYGNGSTNRGREERYRLWFDPSKEFHRYSILWTPTKIIFWVDDVPIREILRKEEMNGDY 217

Query: 219 PSKPMSVFVTIWDGSEWATHGGKHPVDYRYAPYVASFEEMEING-SVSPPIA------TV 278
           P KPMS++ TIWD S WAT GGK  VDY ++P+V+ F+++ ++G +VS           +
Sbjct: 218 PQKPMSLYATIWDASSWATSGGKFGVDYTFSPFVSEFKDIALDGCNVSDSFPGENNNNNI 277

Query: 279 PSSSKTNVSSPDT-VEGPEFIKLSQQQIDAMDWARRKLMFYSYCKDTSRYKVLPPEC 325
            + +  N S  D  +   ++  +S +Q  AM   R + M+YSYC DT RY V PPEC
Sbjct: 278 GNYNNINCSVSDQFLMSNDYSTISPKQATAMRRFRERYMYYSYCYDTIRYSVPPPEC 312

BLAST of Carg14351 vs. ExPASy TrEMBL
Match: A0A6J1GYH0 (Xyloglucan endotransglucosylase/hydrolase OS=Cucurbita moschata OX=3662 GN=LOC111458636 PE=3 SV=1)

HSP 1 Score: 542.7 bits (1397), Expect = 1.0e-150
Identity = 270/325 (83.08%), Postives = 281/325 (86.46%), Query Frame = 0

Query: 1   MELPLLSFFLLCMFFAAPSCGTYYTPPSVPRLTDLVPNVSIDQYFAKIFGAANIQLMSNG 60
           MELPLLSFFLLCMFFAAPSCGTYYTPPSVPRLTDLVPNVSIDQYFAKIFGAANIQLMSNG
Sbjct: 1   MELPLLSFFLLCMFFAAPSCGTYYTPPSVPRLTDLVPNVSIDQYFAKIFGAANIQLMSNG 60

Query: 61  SSLNLTLNKHSGKSSLDLSFSFQPDSVLIHNFFSFHFWRCWFGLEEQISLWVLHLVHNQR 120
           SSLNLTLNKHSG              ++  + + + F+     L    +  V+   +   
Sbjct: 61  SSLNLTLNKHSGA------------GLVSRSKYHYGFFSASIKLPSGFTSGVVVAFY--- 120

Query: 121 DDEGHVQLSNADVYTHSHDEIDIELLGHDKRKDWVIQTNMYANGSVKTGREEKFYLWFDP 180
                  LSNADVYTHSHDEIDIELLGHD RKDWVIQTNMYANGSVKTGREEKFYLWFDP
Sbjct: 121 -------LSNADVYTHSHDEIDIELLGHDNRKDWVIQTNMYANGSVKTGREEKFYLWFDP 180

Query: 181 SVKYHDYTIIWNKYHTVFLVDNVPIRELRNSEAFYPSKPMSVFVTIWDGSEWATHGGKHP 240
           SVKYHDYTIIWNKYHTVFLVDNVPIRELRNSEAFYPSKPMSVFVTIWDGSEWATHGGK+P
Sbjct: 181 SVKYHDYTIIWNKYHTVFLVDNVPIRELRNSEAFYPSKPMSVFVTIWDGSEWATHGGKYP 240

Query: 241 VDYRYAPYVASFEEMEINGSVSPPIATVPSSSKTNVSSPDTVEGPEFIKLSQQQIDAMDW 300
           VDYRYAPYVASFEEMEINGSVSPPIATVPSSSKTNVSSPDTVEGPEF+KLSQQQIDAMDW
Sbjct: 241 VDYRYAPYVASFEEMEINGSVSPPIATVPSSSKTNVSSPDTVEGPEFVKLSQQQIDAMDW 300

Query: 301 ARRKLMFYSYCKDTSRYKVLPPECK 326
           ARRKLMFYSYCKDTSRYKVLPPECK
Sbjct: 301 ARRKLMFYSYCKDTSRYKVLPPECK 303

BLAST of Carg14351 vs. ExPASy TrEMBL
Match: A0A6J1J604 (Xyloglucan endotransglucosylase/hydrolase OS=Cucurbita maxima OX=3661 GN=LOC111483755 PE=3 SV=1)

HSP 1 Score: 534.6 bits (1376), Expect = 2.8e-148
Identity = 269/327 (82.26%), Postives = 280/327 (85.63%), Query Frame = 0

Query: 1   MELPL--LSFFLLCMFFAAPSCGTYYTPPSVPRLTDLVPNVSIDQYFAKIFGAANIQLMS 60
           MELPL  LSFFLLCMFFAAPSCGTYYTPPSVPRLTDLVPNVSIDQYFAKIFG ANIQLMS
Sbjct: 1   MELPLLSLSFFLLCMFFAAPSCGTYYTPPSVPRLTDLVPNVSIDQYFAKIFGGANIQLMS 60

Query: 61  NGSSLNLTLNKHSGKSSLDLSFSFQPDSVLIHNFFSFHFWRCWFGLEEQISLWVLHLVHN 120
           NGSSLNLTLNK SG              ++  N + + F+     L    +  V+   + 
Sbjct: 61  NGSSLNLTLNKLSGA------------GLVSRNKYHYGFFSASIKLPSGFTSGVVVAFY- 120

Query: 121 QRDDEGHVQLSNADVYTHSHDEIDIELLGHDKRKDWVIQTNMYANGSVKTGREEKFYLWF 180
                    LSNADVYTHSHDEIDIELLGHDKRKDWVIQTNMYANGSVKTGREEKFYLWF
Sbjct: 121 ---------LSNADVYTHSHDEIDIELLGHDKRKDWVIQTNMYANGSVKTGREEKFYLWF 180

Query: 181 DPSVKYHDYTIIWNKYHTVFLVDNVPIRELRNSEAFYPSKPMSVFVTIWDGSEWATHGGK 240
           DPSVKYHDYTIIWNKYHTVFLVDNVPIRELRNSEAFYPSKPMSVFVTIWDGSEWATHGGK
Sbjct: 181 DPSVKYHDYTIIWNKYHTVFLVDNVPIRELRNSEAFYPSKPMSVFVTIWDGSEWATHGGK 240

Query: 241 HPVDYRYAPYVASFEEMEINGSVSPPIATVPSSSKTNVSSPDTVEGPEFIKLSQQQIDAM 300
           +PVDY+YAPYVASFEEMEINGS+SPPIATVPSSSKTNVSSPDTVEGPEFIKLSQQQIDAM
Sbjct: 241 YPVDYKYAPYVASFEEMEINGSISPPIATVPSSSKTNVSSPDTVEGPEFIKLSQQQIDAM 300

Query: 301 DWARRKLMFYSYCKDTSRYKVLPPECK 326
           DWARRKLMFYSYCKDTSRYKVLPPECK
Sbjct: 301 DWARRKLMFYSYCKDTSRYKVLPPECK 305

BLAST of Carg14351 vs. ExPASy TrEMBL
Match: A0A6J1JER7 (Xyloglucan endotransglucosylase/hydrolase OS=Cucurbita maxima OX=3661 GN=LOC111483755 PE=3 SV=1)

HSP 1 Score: 534.6 bits (1376), Expect = 2.8e-148
Identity = 269/327 (82.26%), Postives = 280/327 (85.63%), Query Frame = 0

Query: 1   MELPL--LSFFLLCMFFAAPSCGTYYTPPSVPRLTDLVPNVSIDQYFAKIFGAANIQLMS 60
           MELPL  LSFFLLCMFFAAPSCGTYYTPPSVPRLTDLVPNVSIDQYFAKIFG ANIQLMS
Sbjct: 22  MELPLLSLSFFLLCMFFAAPSCGTYYTPPSVPRLTDLVPNVSIDQYFAKIFGGANIQLMS 81

Query: 61  NGSSLNLTLNKHSGKSSLDLSFSFQPDSVLIHNFFSFHFWRCWFGLEEQISLWVLHLVHN 120
           NGSSLNLTLNK SG              ++  N + + F+     L    +  V+   + 
Sbjct: 82  NGSSLNLTLNKLSGA------------GLVSRNKYHYGFFSASIKLPSGFTSGVVVAFY- 141

Query: 121 QRDDEGHVQLSNADVYTHSHDEIDIELLGHDKRKDWVIQTNMYANGSVKTGREEKFYLWF 180
                    LSNADVYTHSHDEIDIELLGHDKRKDWVIQTNMYANGSVKTGREEKFYLWF
Sbjct: 142 ---------LSNADVYTHSHDEIDIELLGHDKRKDWVIQTNMYANGSVKTGREEKFYLWF 201

Query: 181 DPSVKYHDYTIIWNKYHTVFLVDNVPIRELRNSEAFYPSKPMSVFVTIWDGSEWATHGGK 240
           DPSVKYHDYTIIWNKYHTVFLVDNVPIRELRNSEAFYPSKPMSVFVTIWDGSEWATHGGK
Sbjct: 202 DPSVKYHDYTIIWNKYHTVFLVDNVPIRELRNSEAFYPSKPMSVFVTIWDGSEWATHGGK 261

Query: 241 HPVDYRYAPYVASFEEMEINGSVSPPIATVPSSSKTNVSSPDTVEGPEFIKLSQQQIDAM 300
           +PVDY+YAPYVASFEEMEINGS+SPPIATVPSSSKTNVSSPDTVEGPEFIKLSQQQIDAM
Sbjct: 262 YPVDYKYAPYVASFEEMEINGSISPPIATVPSSSKTNVSSPDTVEGPEFIKLSQQQIDAM 321

Query: 301 DWARRKLMFYSYCKDTSRYKVLPPECK 326
           DWARRKLMFYSYCKDTSRYKVLPPECK
Sbjct: 322 DWARRKLMFYSYCKDTSRYKVLPPECK 326

BLAST of Carg14351 vs. ExPASy TrEMBL
Match: N0DX87 (Xyloglucan endotransglucosylase/hydrolase OS=Cucumis sativus OX=3659 GN=xth29 PE=3 SV=1)

HSP 1 Score: 485.7 bits (1249), Expect = 1.5e-133
Identity = 239/325 (73.54%), Postives = 261/325 (80.31%), Query Frame = 0

Query: 1   MELPLLSFFLLCMFFAAPSCGTYYTPPSVPRLTDLVPNVSIDQYFAKIFGAANIQLMSNG 60
           MELPLLSF  LCM F APSC  YYTPPSVPRLTDLVP VS DQ FAKIFGA+NIQL +NG
Sbjct: 1   MELPLLSFIFLCMLFVAPSCSRYYTPPSVPRLTDLVPRVSTDQCFAKIFGASNIQLRNNG 60

Query: 61  SSLNLTLNKHSGKSSLDLSFSFQPDSVLIHNFFSFHFWRCWFGLEEQISLWVLHLVHNQR 120
           SS++LTL+K SG              ++  N + + F+     L   ++  V+   +   
Sbjct: 61  SSVDLTLDKVSGA------------GLVSRNKYHYGFFSASIKLPSGLTSGVVVAFY--- 120

Query: 121 DDEGHVQLSNADVYTHSHDEIDIELLGHDKRKDWVIQTNMYANGSVKTGREEKFYLWFDP 180
                  LSNADVY HSHDEIDIELLGHDKRKDWVIQTN+YANGSVKTGREEKFYLWFDP
Sbjct: 121 -------LSNADVYPHSHDEIDIELLGHDKRKDWVIQTNIYANGSVKTGREEKFYLWFDP 180

Query: 181 SVKYHDYTIIWNKYHTVFLVDNVPIRELRNSEAFYPSKPMSVFVTIWDGSEWATHGGKHP 240
           S+KYHDYTIIWN YHTVFLVDNVP+RELRNSE FYPSKPMSVFVTIWDGSEWATHGGK+P
Sbjct: 181 SLKYHDYTIIWNNYHTVFLVDNVPVRELRNSEVFYPSKPMSVFVTIWDGSEWATHGGKYP 240

Query: 241 VDYRYAPYVASFEEMEINGSVSPPIATVPSSSKTNVSSPDTVEGPEFIKLSQQQIDAMDW 300
           VDY++APY ASFEEMEING +  P ATVPSSSK NVS PDT EGPEFIKLSQQQ+DAMDW
Sbjct: 241 VDYKHAPYTASFEEMEINGGILTPTATVPSSSKANVSGPDTAEGPEFIKLSQQQVDAMDW 300

Query: 301 ARRKLMFYSYCKDTSRYKVLPPECK 326
           ARRKLMFYSYCKDTSRYKVLPPECK
Sbjct: 301 ARRKLMFYSYCKDTSRYKVLPPECK 303

BLAST of Carg14351 vs. ExPASy TrEMBL
Match: A0A0A0KQA3 (Xyloglucan endotransglucosylase/hydrolase OS=Cucumis sativus OX=3659 GN=Csa_5G602120 PE=3 SV=1)

HSP 1 Score: 485.0 bits (1247), Expect = 2.5e-133
Identity = 239/325 (73.54%), Postives = 261/325 (80.31%), Query Frame = 0

Query: 1   MELPLLSFFLLCMFFAAPSCGTYYTPPSVPRLTDLVPNVSIDQYFAKIFGAANIQLMSNG 60
           MELPLLSF  LCM F APSC  YYTPPSVPRLTDLVP VS DQ FAKIFGA+NIQL +NG
Sbjct: 8   MELPLLSFIFLCMLFVAPSCSRYYTPPSVPRLTDLVPRVSTDQCFAKIFGASNIQLRNNG 67

Query: 61  SSLNLTLNKHSGKSSLDLSFSFQPDSVLIHNFFSFHFWRCWFGLEEQISLWVLHLVHNQR 120
           SS++LTL+K SG              ++  N + + F+     L   ++  V+   +   
Sbjct: 68  SSVDLTLDKVSGA------------GLVSRNKYHYGFFSASIKLPSGLTSGVVVAFY--- 127

Query: 121 DDEGHVQLSNADVYTHSHDEIDIELLGHDKRKDWVIQTNMYANGSVKTGREEKFYLWFDP 180
                  LSNADVY HSHDEIDIELLGHDKRKDWVIQTN+YANGSVKTGREEKFYLWFDP
Sbjct: 128 -------LSNADVYPHSHDEIDIELLGHDKRKDWVIQTNIYANGSVKTGREEKFYLWFDP 187

Query: 181 SVKYHDYTIIWNKYHTVFLVDNVPIRELRNSEAFYPSKPMSVFVTIWDGSEWATHGGKHP 240
           S+KYHDYTIIWN YHTVFLVDNVP+RELRNSE FYPSKPMSVFVTIWDGSEWATHGGK+P
Sbjct: 188 SLKYHDYTIIWNNYHTVFLVDNVPVRELRNSEVFYPSKPMSVFVTIWDGSEWATHGGKYP 247

Query: 241 VDYRYAPYVASFEEMEINGSVSPPIATVPSSSKTNVSSPDTVEGPEFIKLSQQQIDAMDW 300
           VDY++APY ASFEEMEING +  P ATVPSSSK NVS PDT EGPEFIKLSQQQ+DAMDW
Sbjct: 248 VDYKHAPYTASFEEMEINGGILTPKATVPSSSKANVSGPDTAEGPEFIKLSQQQVDAMDW 307

Query: 301 ARRKLMFYSYCKDTSRYKVLPPECK 326
           ARRKLMFYSYCKDTSRYKVLPPECK
Sbjct: 308 ARRKLMFYSYCKDTSRYKVLPPECK 310

BLAST of Carg14351 vs. TAIR 10
Match: AT1G10550.1 (xyloglucan:xyloglucosyl transferase 33 )

HSP 1 Score: 299.7 bits (766), Expect = 2.9e-81
Identity = 159/323 (49.23%), Postives = 208/323 (64.40%), Query Frame = 0

Query: 9   FLLCMFFAAPSCGTYYTPPSVPRLTDLVPNVSIDQYFAKIFGAANIQLMSNGSSLNLTLN 68
           F LC      S    +T P+V RLTD    ++I+  F++ FGA NIQ+  NGS   LTL+
Sbjct: 11  FCLCSLSLVSSHSRKFTTPNVTRLTDQFSKIAIENGFSRRFGAHNIQV--NGSLAKLTLD 70

Query: 69  KHSGKSSLDLSFSFQPDSVLIHNFFSFHFWRCWFGLEEQISLWVLHLVHNQRDDEGHVQL 128
           K SG              ++  N + + F+     L    +  V+   +          L
Sbjct: 71  KSSGA------------GLVSKNKYHYGFFSARLKLPAGFASGVVVAFY----------L 130

Query: 129 SNADVYTHSHDEIDIELLGHDKRKDWVIQTNMYANGSVKTGREEKFYLWFDPSVKYHDYT 188
           SNA+ Y  SHDEIDIELLG  +R DW IQTN+YANGS +TGREEKFY WFDP+  +HDYT
Sbjct: 131 SNAETYPKSHDEIDIELLGRSRRDDWTIQTNVYANGSTRTGREEKFYFWFDPTQAFHDYT 190

Query: 189 IIWNKYHTVFLVDNVPIRELRNSEAF---YPSKPMSVFVTIWDGSEWATHGGKHPVDYRY 248
           +IWN +HTVFLVDN+P+R+  N  AF   YPSKPMS++VT+WDGSEWAT GGK+PV+Y+Y
Sbjct: 191 LIWNSHHTVFLVDNIPVRQFPNRGAFTSAYPSKPMSLYVTVWDGSEWATKGGKYPVNYKY 250

Query: 249 APYVASFEEMEING-SVSPPIATVP---SSSKTNVSSPDTVEGPEFIKLSQQQIDAMDWA 308
           AP+V S  ++E++G SV+   +T     + S  ++SS D V+G +F  LS+ QI+AMDWA
Sbjct: 251 APFVVSVADVELSGCSVNNGSSTGSGPCTKSGGSISSLDPVDGQDFATLSKNQINAMDWA 309

Query: 309 RRKLMFYSYCKDTSRYKVLPPEC 325
           RRKLMFYSYC D  RYKV+P EC
Sbjct: 311 RRKLMFYSYCSDKPRYKVMPAEC 309

BLAST of Carg14351 vs. TAIR 10
Match: AT2G01850.1 (endoxyloglucan transferase A3 )

HSP 1 Score: 214.9 bits (546), Expect = 9.4e-56
Identity = 111/292 (38.01%), Postives = 171/292 (58.56%), Query Frame = 0

Query: 36  VPNVSIDQYFAKIFGAANIQLMSNGSSLNLTLNKHSGKSSLDLSFSFQPDSVLIHNFFSF 95
           +P  S ++ + ++FG  N+ +  +G S+ LTL++ +G         F  +   +H FFS 
Sbjct: 26  LPITSFEESYTQLFGDKNLFVHQDGKSVRLTLDERTGS-------GFVSNDYYLHGFFS- 85

Query: 96  HFWRCWFGLEEQISLWVLHLVHNQRDDEGHVQLSNADVYTHSHDEIDIELLGHDKRKDWV 155
                   L    +  V+   +          +SN D+Y  +HDEID E LG+ + K+W 
Sbjct: 86  ----ASIKLPSDYTAGVVVAFY----------MSNGDMYEKNHDEIDFEFLGNIREKEWR 145

Query: 156 IQTNMYANGSVKTGREEKFYLWFDPSVKYHDYTIIWNKYHTVFLVDNVPIRELRNSE--- 215
           +QTN+Y NGS  +GREE++ LWFDP+  +H Y+I+W+  H +F VDNVPIRE++ +    
Sbjct: 146 VQTNIYGNGSTHSGREERYNLWFDPTEDFHQYSILWSDSHIIFFVDNVPIREVKRTAEMG 205

Query: 216 AFYPSKPMSVFVTIWDGSEWATHGGKHPVDYRYAPYVASFEEMEINGSVSPPIATVPSSS 275
             +PSKPMS++ TIWDGS+WAT+GGK+ V+Y+YAPY+A F ++ ++G    PI   P   
Sbjct: 206 GHFPSKPMSLYTTIWDGSKWATNGGKYGVNYKYAPYIARFSDLVLHGCPVDPIEQFP--- 265

Query: 276 KTNVSSPDTVEGPEFIKLSQQQIDAMDWARRKLMFYSYCKDTSRYKVLPPEC 325
           + +  + + +   + I  SQ+    MD  RR+LM YSYC D +RY V   EC
Sbjct: 266 RCDEGAAEDMRAAQEITPSQR--SKMDVFRRRLMTYSYCYDRARYNVALSEC 290

BLAST of Carg14351 vs. TAIR 10
Match: AT1G14720.1 (xyloglucan endotransglucosylase/hydrolase 28 )

HSP 1 Score: 210.3 bits (534), Expect = 2.3e-54
Identity = 110/292 (37.67%), Postives = 169/292 (57.88%), Query Frame = 0

Query: 36  VPNVSIDQYFAKIFGAANIQLMSNGSSLNLTLNKHSGKSSLDLSFSFQPDSVLIHNFFSF 95
           +P +  D+ + ++FG  N+ +  +G S+ LTL++ +G         F  + + +H FFS 
Sbjct: 26  LPLIQFDEGYTQLFGDQNLIVHRDGKSVRLTLDERTGS-------GFVSNDIYLHGFFSS 85

Query: 96  HFWRCWFGLEEQISLWVLHLVHNQRDDEGHVQLSNADVYTHSHDEIDIELLGHDKRKDWV 155
                   L    S  V+   +          LSN D+Y  +HDEID E LG+ + ++W 
Sbjct: 86  S-----IKLPADYSAGVVIAFY----------LSNGDLYEKNHDEIDFEFLGNIRGREWR 145

Query: 156 IQTNMYANGSVKTGREEKFYLWFDPSVKYHDYTIIWNKYHTVFLVDNVPIRELRNSEAF- 215
           IQTN+Y NGS   GREE++ LWFDP+  +H Y+I+W+  H +F VDNVPIRE++ + +  
Sbjct: 146 IQTNIYGNGSTHLGREERYNLWFDPTEDFHQYSILWSLSHIIFYVDNVPIREVKRTASMG 205

Query: 216 --YPSKPMSVFVTIWDGSEWATHGGKHPVDYRYAPYVASFEEMEINGSVSPPIATVPSSS 275
             +P+KPMS++ TIWDGS+WAT GGK+ V+Y+YAPYV+ F ++ ++G    P    PS  
Sbjct: 206 GDFPAKPMSLYSTIWDGSKWATDGGKYGVNYKYAPYVSQFTDLILHGCAVDPTEKFPSCK 265

Query: 276 KTNVSSPDTVEGPEFIKLSQQQIDAMDWARRKLMFYSYCKDTSRYKVLPPEC 325
              V +          ++++ Q + M+  R+K M YSYC D  RYKV+  EC
Sbjct: 266 DEAVQNLRLAS-----EITESQRNKMEIFRQKHMTYSYCYDHMRYKVVLSEC 290

BLAST of Carg14351 vs. TAIR 10
Match: AT1G32170.1 (xyloglucan endotransglucosylase/hydrolase 30 )

HSP 1 Score: 200.3 bits (508), Expect = 2.4e-51
Identity = 104/289 (35.99%), Postives = 161/289 (55.71%), Query Frame = 0

Query: 39  VSIDQYFAKIFGAANIQLMSNGSSLNLTLNKHSGKSSLDLSFSFQPDSVLIHNFFSFHFW 98
           +S ++  + +FG AN+    +  S+ L L++++G   +              N +   F+
Sbjct: 30  LSFEESLSPLFGDANLVRSPDDLSVRLLLDRYTGSGFIS------------SNMYQHGFY 89

Query: 99  RCWFGLEEQISLWVLHLVHNQRDDEGHVQLSNADVYTHSHDEIDIELLGHDKRKDWVIQT 158
                L    +  V+   +           SN DV+  +HDE+DIE LG+ K K W  QT
Sbjct: 90  SSMIKLPADYTAGVVVAFYT----------SNGDVFEKTHDELDIEFLGNIKGKPWRFQT 149

Query: 159 NMYANGSVKTGREEKFYLWFDPSVKYHDYTIIWNKYHTVFLVDNVPIRELRNSEAF---Y 218
           N+Y NGS   GREE++ LWFDPS ++H Y+I+W  +  +F VD+VPIRE+  ++A    Y
Sbjct: 150 NLYGNGSTHRGREERYRLWFDPSKEFHRYSILWTPHKIIFWVDDVPIREVIRNDAMGADY 209

Query: 219 PSKPMSVFVTIWDGSEWATHGGKHPVDYRYAPYVASFEEMEINGSVSPPIATVPSSSKTN 278
           P+KPM+++ TIWD S+WAT GGK+  +Y++AP+VA F+   ++G    PI  VP     +
Sbjct: 210 PAKPMALYATIWDASDWATSGGKYKANYKFAPFVAEFKSFSLDGCSVDPIQEVPMDCSDS 269

Query: 279 VSSPDTVEGPEFIKLSQQQIDAMDWARRKLMFYSYCKDTSRYKVLPPEC 325
           V   D +E  ++  ++  Q  AM   R++ M+YSYC DT RY    PEC
Sbjct: 270 V---DFLESQDYSSINSHQRAAMRRFRQRFMYYSYCYDTLRYPEPLPEC 293

BLAST of Carg14351 vs. TAIR 10
Match: AT4G18990.1 (xyloglucan endotransglucosylase/hydrolase 29 )

HSP 1 Score: 191.8 bits (486), Expect = 8.5e-49
Identity = 110/297 (37.04%), Postives = 159/297 (53.54%), Query Frame = 0

Query: 39  VSIDQYFAKIFGAANIQLMSNGSSLNLTLNKHSGKSSLDLSFSFQPDSVLIHNFFSFHFW 98
           +  D+  + +FG  N+    +  S+ L L+K++G         F   S+  H FFS    
Sbjct: 38  IFFDEGLSHLFGEGNLIRSPDDRSVRLLLDKYTGS-------GFISSSMYQHGFFS---- 97

Query: 99  RCWFGLEEQISLWVLHLVHNQRDDEGHVQLSNADVYTHSHDEIDIELLGHDKRKDWVIQT 158
                L +    +   +V            SN DV+   HDE+DIE LG+ + K W  QT
Sbjct: 98  ----SLIKLPGAYTAGIV-------VAFYTSNGDVFVKDHDELDIEFLGNLEGKPWRFQT 157

Query: 159 NMYANGSVKTGREEKFYLWFDPSVKYHDYTIIWNKYHTVFLVDNVPIRELRNSEAF---Y 218
           NMY NGS   GREE++ LWFDPS ++H Y+I+W     +F VD+VPIRE+   E     Y
Sbjct: 158 NMYGNGSTNRGREERYRLWFDPSKEFHRYSILWTPTKIIFWVDDVPIREILRKEEMNGDY 217

Query: 219 PSKPMSVFVTIWDGSEWATHGGKHPVDYRYAPYVASFEEMEING-SVSPPIA------TV 278
           P KPMS++ TIWD S WAT GGK  VDY ++P+V+ F+++ ++G +VS           +
Sbjct: 218 PQKPMSLYATIWDASSWATSGGKFGVDYTFSPFVSEFKDIALDGCNVSDSFPGENNNNNI 277

Query: 279 PSSSKTNVSSPDT-VEGPEFIKLSQQQIDAMDWARRKLMFYSYCKDTSRYKVLPPEC 325
            + +  N S  D  +   ++  +S +Q  AM   R + M+YSYC DT RY V PPEC
Sbjct: 278 GNYNNINCSVSDQFLMSNDYSTISPKQATAMRRFRERYMYYSYCYDTIRYSVPPPEC 312

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
KAG7032271.12.9e-192100.00putative xyloglucan endotransglucosylase/hydrolase protein 33, partial [Cucurbit... [more]
KAG6601490.15.9e-16191.29putative xyloglucan endotransglucosylase/hydrolase protein 33, partial [Cucurbit... [more]
XP_022957166.12.1e-15083.08probable xyloglucan endotransglucosylase/hydrolase protein 33 [Cucurbita moschat... [more]
XP_023512499.11.2e-14882.15probable xyloglucan endotransglucosylase/hydrolase protein 33 [Cucurbita pepo su... [more]
XP_022985837.15.8e-14882.26probable xyloglucan endotransglucosylase/hydrolase protein 33 isoform X2 [Cucurb... [more]
Match NameE-valueIdentityDescription
Q8LC454.1e-8049.23Probable xyloglucan endotransglucosylase/hydrolase protein 33 OS=Arabidopsis tha... [more]
Q8LDS21.3e-5438.01Probable xyloglucan endotransglucosylase/hydrolase protein 27 OS=Arabidopsis tha... [more]
Q389093.3e-5337.67Probable xyloglucan endotransglucosylase/hydrolase protein 28 OS=Arabidopsis tha... [more]
Q389083.4e-5035.99Probable xyloglucan endotransglucosylase/hydrolase protein 30 OS=Arabidopsis tha... [more]
Q8L7H31.2e-4737.04Probable xyloglucan endotransglucosylase/hydrolase protein 29 OS=Arabidopsis tha... [more]
Match NameE-valueIdentityDescription
A0A6J1GYH01.0e-15083.08Xyloglucan endotransglucosylase/hydrolase OS=Cucurbita moschata OX=3662 GN=LOC11... [more]
A0A6J1J6042.8e-14882.26Xyloglucan endotransglucosylase/hydrolase OS=Cucurbita maxima OX=3661 GN=LOC1114... [more]
A0A6J1JER72.8e-14882.26Xyloglucan endotransglucosylase/hydrolase OS=Cucurbita maxima OX=3661 GN=LOC1114... [more]
N0DX871.5e-13373.54Xyloglucan endotransglucosylase/hydrolase OS=Cucumis sativus OX=3659 GN=xth29 PE... [more]
A0A0A0KQA32.5e-13373.54Xyloglucan endotransglucosylase/hydrolase OS=Cucumis sativus OX=3659 GN=Csa_5G60... [more]
Match NameE-valueIdentityDescription
AT1G10550.12.9e-8149.23xyloglucan:xyloglucosyl transferase 33 [more]
AT2G01850.19.4e-5638.01endoxyloglucan transferase A3 [more]
AT1G14720.12.3e-5437.67xyloglucan endotransglucosylase/hydrolase 28 [more]
AT1G32170.12.4e-5135.99xyloglucan endotransglucosylase/hydrolase 30 [more]
AT4G18990.18.5e-4937.04xyloglucan endotransglucosylase/hydrolase 29 [more]
InterPro
Analysis Name: InterPro Annotations of Silver-seed gourd (SMH-JMG-627) v2
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR000757Glycoside hydrolase family 16PFAMPF00722Glyco_hydro_16coord: 132..244
e-value: 2.5E-32
score: 111.8
IPR000757Glycoside hydrolase family 16PROSITEPS51762GH16_2coord: 22..252
score: 19.382275
IPR016455Xyloglucan endotransglucosylase/hydrolasePIRSFPIRSF005604EndGlu_transfcoord: 8..88
e-value: 0.055
score: 10.3
coord: 124..325
e-value: 2.6E-74
score: 247.9
NoneNo IPR availableGENE3D2.60.120.200coord: 26..324
e-value: 9.7E-64
score: 217.3
NoneNo IPR availablePANTHERPTHR31062:SF144XYLOGLUCAN ENDOTRANSGLUCOSYLASE/HYDROLASE PROTEIN 33-RELATEDcoord: 128..325
NoneNo IPR availablePANTHERPTHR31062:SF144XYLOGLUCAN ENDOTRANSGLUCOSYLASE/HYDROLASE PROTEIN 33-RELATEDcoord: 4..73
IPR010713Xyloglucan endo-transglycosylase, C-terminalPFAMPF06955XET_Ccoord: 289..324
e-value: 4.1E-14
score: 52.1
IPR044791Beta-glucanase/XTHPANTHERPTHR31062XYLOGLUCAN ENDOTRANSGLUCOSYLASE/HYDROLASE PROTEIN 8-RELATEDcoord: 4..73
IPR044791Beta-glucanase/XTHPANTHERPTHR31062XYLOGLUCAN ENDOTRANSGLUCOSYLASE/HYDROLASE PROTEIN 8-RELATEDcoord: 128..325
IPR013320Concanavalin A-like lectin/glucanase domain superfamilySUPERFAMILY49899Concanavalin A-like lectins/glucanasescoord: 129..324

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Carg14351-RACarg14351-RAmRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0042546 cell wall biogenesis
biological_process GO:0009831 plant-type cell wall modification involved in multidimensional cell growth
biological_process GO:0010411 xyloglucan metabolic process
biological_process GO:0005975 carbohydrate metabolic process
biological_process GO:0006073 cellular glucan metabolic process
cellular_component GO:0048046 apoplast
cellular_component GO:0005618 cell wall
cellular_component GO:0005887 integral component of plasma membrane
molecular_function GO:0004553 hydrolase activity, hydrolyzing O-glycosyl compounds
molecular_function GO:0016762 xyloglucan:xyloglucosyl transferase activity