Carg14292 (gene) Silver-seed gourd (SMH-JMG-627) v2

Overview
NameCarg14292
Typegene
OrganismCucurbita argyrosperma subsp. argyrosperma cv. SMH-JMG-627 (Silver-seed gourd (SMH-JMG-627) v2)
DescriptionVery-long-chain 3-oxoacyl-CoA reductase-like protein
LocationCarg_Chr04: 10520448 .. 10522419 (-)
RNA-Seq ExpressionCarg14292
SyntenyCarg14292
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideexonCDSthree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGCACACAATGAGGGAGACATTCTTTCTGTTACTCTCCACTCAGCCCATTTGGCTTCTCCTCCTCTCCTTGCTTGGTTTCTTCACCATTCTCAGGCACTCTACCACTGTTGTTCAATGGGTTTTCAGGGTCTTTCTCAGACCCTCCAAGGATCTGAGATACTATGGCTCTTGGGGAGTAGTCACCGGAGCCACCGATGGCATTGGCAAGTCATTTGCCTATCAGCTGGCCAGAGCCGGCCTCAACCTTATACTGGTGAGCAGAAGCTCCACCAAGCTGAAGGCAGTTTGTAAGGATATCCGATCTCAGTTTCCTAATACAAAGGTAAAGATCATCGAACTGGACTTCACAGAAGATGATATTAGTGCTGGTATGGCAGAGTTTAAGAAGGTGATTCAAGATTTGGACCTGGGGGTTCTGATAAACAACGTGGGGATTACATATCCAAACGCCAGTTTTTTCCATGAAGTGGATGAGAAAGTCTGGATGAATGTTCTTAAAGTGAACGTGAAGGGCACTACTTGGGTTACCAAAGCTGTTTTGCCAAAGATGATTACTAAGAACAGAGGAGCTATTGTGAATATTGGCTCTGGAGCTGCTGTTATTGTTCCTTCTCATCCTCTCTACGCAATCTATGCTGCTACAAAAGCGTAAGTAATCAAGTTCCCAACTTTCTTCTTCAGTTTATTTTCTTGTTCTTGTGCTTCTTTTTACTTTTCAGGAACTCGTAATCATGGAGGGAAAAGAAATCAAGAAAATTTGAGGAAAAAAGCCCATCTCTTTCTATCACTTCAGAAGAACTGATTAATGAGATGTGGCTCAGCAACTGCCCATGTCTTCCTGCCAAACCAACTTTGAGAAACTTTTAACTTCTCTGCATTTTGAGATTAAGAAAGTACAGGTACAGGTTTGGCAGATGGGTTCAGTTTGTATTAGAAGAACGTGCCGTTGACTGTTATGGCCGTCTGTTATGGCCGTCGCTAGCAAATATGGTCTTCTTTGGGCTTTTCCTTTTAGGTTTCTCCTCAAGGTTTTTAAAATACAGTCTGTTAGGGAGATGTTTCCACACCCTTAATGCTTCGTTCCTCTCTCCAACCAATGTGGGATCCCACATGTTTAATGAAACCCAACTCTGTATCCCAACCACACATCGATTCCCACTTCCACGGCTAAGTTAAAACCTTCACTTTTCTGATGATGGGATGGTCGAAAAGCTCAAAAACTTTGATCTTGTTGATGACAGAATCTGATGGGTTCTTTTGTTGTATTCAGGTACGTGGACCAACTCTCAAGATCTCTACATGTGGAATACAAGCATTGGGGAATTGATGTGCAGTGTCAGGTTAGTATAACACGAACTCATTTTGTTACAACTCGGCTGAAAGATGAAAAATTTGTGTGATCTTTTTAAGAAAATCCAGGTGCCATTGTATGTGGCAACTGAAATGGCGTCGAGAGTGGCGTCGGTTAGCGGAGCGTCGCTGTTCATTCCATCAGCCGATGACTATGTGAGTGCTGCAATTCACAGAATTGGATACGAGCCACGATGCACCCCTTACTGGGCTCACTCTCTCCAATGGTGCTTTGCTCGTCTTCTCCCGGAGGCAGCGCTCGACGCGTGGCGACTGTCCATTGGCTTGCATAGGAGGAGTAAGGGATTGGACGCCATGAAAGGGATTGAAGGTTACAAACAAGAATAAAAGCAACTCTGTGAAGGCCACCGTTGTATTGCAACTACAAGCTATGATGATATAGTAAATGTGTTGTAATAGTCATGAATGTGATGAGTTGTTCAATAAGGTGATATAATATGGTTTGCTCGACTTCAATGTTGCCAAGCTCATTCACGTCTTGTTGGTACTGTGACCTATCAATTATTTAATGTCCTCTCAATCCGAGCGTGATTCAATAAAAAAAAGACGCTAATTCTTAAAAAAAAAAGTGAAAAAGTGGAACTTAGTCCTTATTTTAT

mRNA sequence

ATGCACACAATGAGGGAGACATTCTTTCTGTTACTCTCCACTCAGCCCATTTGGCTTCTCCTCCTCTCCTTGCTTGGTTTCTTCACCATTCTCAGGCACTCTACCACTGTTGTTCAATGGGTTTTCAGGGTCTTTCTCAGACCCTCCAAGGATCTGAGATACTATGGCTCTTGGGGAGTAGTCACCGGAGCCACCGATGGCATTGGCAAGTCATTTGCCTATCAGCTGGCCAGAGCCGGCCTCAACCTTATACTGGTGAGCAGAAGCTCCACCAAGCTGAAGGCAGTTTGTAAGGATATCCGATCTCAGTTTCCTAATACAAAGGTAAAGATCATCGAACTGGACTTCACAGAAGATGATATTAGTGCTGGTATGGCAGAGTTTAAGAAGGTGATTCAAGATTTGGACCTGGGGGTTCTGATAAACAACGTGGGGATTACATATCCAAACGCCAGTTTTTTCCATGAAGTGGATGAGAAAGTCTGGATGAATGTTCTTAAAGTGAACGTGAAGGGCACTACTTGGGTTACCAAAGCTGTTTTGCCAAAGATGATTACTAAGAACAGAGGAGCTATTGTGAATATTGGCTCTGGAGCTGCTGTTATTGTTCCTTCTCATCCTCTCTACGCAATCTATGCTGCTACAAAAGCGTACGTGGACCAACTCTCAAGATCTCTACATGTGGAATACAAGCATTGGGGAATTGATGTGCAGTGTCAGGTGCCATTGTATGTGGCAACTGAAATGGCGTCGAGAGTGGCGTCGGTTAGCGGAGCGTCGCTGTTCATTCCATCAGCCGATGACTATGTGAGTGCTGCAATTCACAGAATTGGATACGAGCCACGATGCACCCCTTACTGGGCTCACTCTCTCCAATGGTGCTTTGCTCGTCTTCTCCCGGAGGCAGCGCTCGACGCGTGGCGACTGTCCATTGGCTTGCATAGGAGGAGTAAGGGATTGGACGCCATGAAAGGGATTGAAGGTTACAAACAAGAATAAAAGCAACTCTGTGAAGGCCACCGTTGTATTGCAACTACAAGCTATGATGATATAGTAAATGTGTTGTAATAGTCATGAATGTGATGAGTTGTTCAATAAGGTGATATAATATGGTTTGCTCGACTTCAATGTTGCCAAGCTCATTCACGTCTTGTTGGTACTGTGACCTATCAATTATTTAATGTCCTCTCAATCCGAGCGTGATTCAATAAAAAAAAGACGCTAATTCTTAAAAAAAAAAGTGAAAAAGTGGAACTTAGTCCTTATTTTAT

Coding sequence (CDS)

ATGCACACAATGAGGGAGACATTCTTTCTGTTACTCTCCACTCAGCCCATTTGGCTTCTCCTCCTCTCCTTGCTTGGTTTCTTCACCATTCTCAGGCACTCTACCACTGTTGTTCAATGGGTTTTCAGGGTCTTTCTCAGACCCTCCAAGGATCTGAGATACTATGGCTCTTGGGGAGTAGTCACCGGAGCCACCGATGGCATTGGCAAGTCATTTGCCTATCAGCTGGCCAGAGCCGGCCTCAACCTTATACTGGTGAGCAGAAGCTCCACCAAGCTGAAGGCAGTTTGTAAGGATATCCGATCTCAGTTTCCTAATACAAAGGTAAAGATCATCGAACTGGACTTCACAGAAGATGATATTAGTGCTGGTATGGCAGAGTTTAAGAAGGTGATTCAAGATTTGGACCTGGGGGTTCTGATAAACAACGTGGGGATTACATATCCAAACGCCAGTTTTTTCCATGAAGTGGATGAGAAAGTCTGGATGAATGTTCTTAAAGTGAACGTGAAGGGCACTACTTGGGTTACCAAAGCTGTTTTGCCAAAGATGATTACTAAGAACAGAGGAGCTATTGTGAATATTGGCTCTGGAGCTGCTGTTATTGTTCCTTCTCATCCTCTCTACGCAATCTATGCTGCTACAAAAGCGTACGTGGACCAACTCTCAAGATCTCTACATGTGGAATACAAGCATTGGGGAATTGATGTGCAGTGTCAGGTGCCATTGTATGTGGCAACTGAAATGGCGTCGAGAGTGGCGTCGGTTAGCGGAGCGTCGCTGTTCATTCCATCAGCCGATGACTATGTGAGTGCTGCAATTCACAGAATTGGATACGAGCCACGATGCACCCCTTACTGGGCTCACTCTCTCCAATGGTGCTTTGCTCGTCTTCTCCCGGAGGCAGCGCTCGACGCGTGGCGACTGTCCATTGGCTTGCATAGGAGGAGTAAGGGATTGGACGCCATGAAAGGGATTGAAGGTTACAAACAAGAATAA

Protein sequence

MHTMRETFFLLLSTQPIWLLLLSLLGFFTILRHSTTVVQWVFRVFLRPSKDLRYYGSWGVVTGATDGIGKSFAYQLARAGLNLILVSRSSTKLKAVCKDIRSQFPNTKVKIIELDFTEDDISAGMAEFKKVIQDLDLGVLINNVGITYPNASFFHEVDEKVWMNVLKVNVKGTTWVTKAVLPKMITKNRGAIVNIGSGAAVIVPSHPLYAIYAATKAYVDQLSRSLHVEYKHWGIDVQCQVPLYVATEMASRVASVSGASLFIPSADDYVSAAIHRIGYEPRCTPYWAHSLQWCFARLLPEAALDAWRLSIGLHRRSKGLDAMKGIEGYKQE
Homology
BLAST of Carg14292 vs. NCBI nr
Match: KAG6601567.1 (Very-long-chain 3-oxoacyl-CoA reductase-like protein, partial [Cucurbita argyrosperma subsp. sororia] >KAG7032329.1 Very-long-chain 3-oxoacyl-CoA reductase-like protein, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 663.7 bits (1711), Expect = 8.4e-187
Identity = 332/332 (100.00%), Postives = 332/332 (100.00%), Query Frame = 0

Query: 1   MHTMRETFFLLLSTQPIWLLLLSLLGFFTILRHSTTVVQWVFRVFLRPSKDLRYYGSWGV 60
           MHTMRETFFLLLSTQPIWLLLLSLLGFFTILRHSTTVVQWVFRVFLRPSKDLRYYGSWGV
Sbjct: 1   MHTMRETFFLLLSTQPIWLLLLSLLGFFTILRHSTTVVQWVFRVFLRPSKDLRYYGSWGV 60

Query: 61  VTGATDGIGKSFAYQLARAGLNLILVSRSSTKLKAVCKDIRSQFPNTKVKIIELDFTEDD 120
           VTGATDGIGKSFAYQLARAGLNLILVSRSSTKLKAVCKDIRSQFPNTKVKIIELDFTEDD
Sbjct: 61  VTGATDGIGKSFAYQLARAGLNLILVSRSSTKLKAVCKDIRSQFPNTKVKIIELDFTEDD 120

Query: 121 ISAGMAEFKKVIQDLDLGVLINNVGITYPNASFFHEVDEKVWMNVLKVNVKGTTWVTKAV 180
           ISAGMAEFKKVIQDLDLGVLINNVGITYPNASFFHEVDEKVWMNVLKVNVKGTTWVTKAV
Sbjct: 121 ISAGMAEFKKVIQDLDLGVLINNVGITYPNASFFHEVDEKVWMNVLKVNVKGTTWVTKAV 180

Query: 181 LPKMITKNRGAIVNIGSGAAVIVPSHPLYAIYAATKAYVDQLSRSLHVEYKHWGIDVQCQ 240
           LPKMITKNRGAIVNIGSGAAVIVPSHPLYAIYAATKAYVDQLSRSLHVEYKHWGIDVQCQ
Sbjct: 181 LPKMITKNRGAIVNIGSGAAVIVPSHPLYAIYAATKAYVDQLSRSLHVEYKHWGIDVQCQ 240

Query: 241 VPLYVATEMASRVASVSGASLFIPSADDYVSAAIHRIGYEPRCTPYWAHSLQWCFARLLP 300
           VPLYVATEMASRVASVSGASLFIPSADDYVSAAIHRIGYEPRCTPYWAHSLQWCFARLLP
Sbjct: 241 VPLYVATEMASRVASVSGASLFIPSADDYVSAAIHRIGYEPRCTPYWAHSLQWCFARLLP 300

Query: 301 EAALDAWRLSIGLHRRSKGLDAMKGIEGYKQE 333
           EAALDAWRLSIGLHRRSKGLDAMKGIEGYKQE
Sbjct: 301 EAALDAWRLSIGLHRRSKGLDAMKGIEGYKQE 332

BLAST of Carg14292 vs. NCBI nr
Match: XP_023517363.1 (very-long-chain 3-oxoacyl-CoA reductase-like protein At1g24470 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 651.4 bits (1679), Expect = 4.3e-183
Identity = 327/332 (98.49%), Postives = 328/332 (98.80%), Query Frame = 0

Query: 1   MHTMRETFFLLLSTQPIWLLLLSLLGFFTILRHSTTVVQWVFRVFLRPSKDLRYYGSWGV 60
           MHTMRETFFLLLSTQPIWLLLLSLLGFFTIL HSTTVVQWVFRVFLRPSKDLRYYGSWGV
Sbjct: 1   MHTMRETFFLLLSTQPIWLLLLSLLGFFTILSHSTTVVQWVFRVFLRPSKDLRYYGSWGV 60

Query: 61  VTGATDGIGKSFAYQLARAGLNLILVSRSSTKLKAVCKDIRSQFPNTKVKIIELDFTEDD 120
           VTGATDGIGKSFAYQLARAGLNLILVSRSSTKLK V KDIRSQFPNTKVKIIELDFTEDD
Sbjct: 61  VTGATDGIGKSFAYQLARAGLNLILVSRSSTKLKEVSKDIRSQFPNTKVKIIELDFTEDD 120

Query: 121 ISAGMAEFKKVIQDLDLGVLINNVGITYPNASFFHEVDEKVWMNVLKVNVKGTTWVTKAV 180
           ISAGMAEFKKVIQDLD+GVLINNVGITYPNASFFHEVDEKVWMNVLKVNVKGTTWVTKAV
Sbjct: 121 ISAGMAEFKKVIQDLDVGVLINNVGITYPNASFFHEVDEKVWMNVLKVNVKGTTWVTKAV 180

Query: 181 LPKMITKNRGAIVNIGSGAAVIVPSHPLYAIYAATKAYVDQLSRSLHVEYKHWGIDVQCQ 240
           LPKMITKNRGAIVNIGSGAAVIVPSHPLYAIYAATKAYVDQLSRSLHVEYKHWGIDVQCQ
Sbjct: 181 LPKMITKNRGAIVNIGSGAAVIVPSHPLYAIYAATKAYVDQLSRSLHVEYKHWGIDVQCQ 240

Query: 241 VPLYVATEMASRVASVSGASLFIPSADDYVSAAIHRIGYEPRCTPYWAHSLQWCFARLLP 300
           VPLYVATEMASRVASVS ASLFIPSADDYVSAAIHRIGYEPRCTPYWAHSLQWCFARLLP
Sbjct: 241 VPLYVATEMASRVASVSRASLFIPSADDYVSAAIHRIGYEPRCTPYWAHSLQWCFARLLP 300

Query: 301 EAALDAWRLSIGLHRRSKGLDAMKGIEGYKQE 333
           EAALDAWRLSIGLHRRSKGLDAMKGIEGYKQE
Sbjct: 301 EAALDAWRLSIGLHRRSKGLDAMKGIEGYKQE 332

BLAST of Carg14292 vs. NCBI nr
Match: XP_022998255.1 (very-long-chain 3-oxoacyl-CoA reductase-like protein At1g24470 [Cucurbita maxima])

HSP 1 Score: 646.4 bits (1666), Expect = 1.4e-181
Identity = 323/332 (97.29%), Postives = 327/332 (98.49%), Query Frame = 0

Query: 1   MHTMRETFFLLLSTQPIWLLLLSLLGFFTILRHSTTVVQWVFRVFLRPSKDLRYYGSWGV 60
           MHTMRETFFLLLSTQPIWLLLLSLLGFFTILRHSTT VQWVFRVFLRPSKDLRYYGSWGV
Sbjct: 1   MHTMRETFFLLLSTQPIWLLLLSLLGFFTILRHSTTAVQWVFRVFLRPSKDLRYYGSWGV 60

Query: 61  VTGATDGIGKSFAYQLARAGLNLILVSRSSTKLKAVCKDIRSQFPNTKVKIIELDFTEDD 120
           VTGATDGIGKSF+YQLARAGLNLILVSRSSTKLKAV KDIRSQFPNTKVKIIELDFTEDD
Sbjct: 61  VTGATDGIGKSFSYQLARAGLNLILVSRSSTKLKAVSKDIRSQFPNTKVKIIELDFTEDD 120

Query: 121 ISAGMAEFKKVIQDLDLGVLINNVGITYPNASFFHEVDEKVWMNVLKVNVKGTTWVTKAV 180
           ISAGMAEFKKVIQDLD+GVLINNVGITYPNASFFHEVDEKVWMNVLKVNVKGTTWVTKAV
Sbjct: 121 ISAGMAEFKKVIQDLDVGVLINNVGITYPNASFFHEVDEKVWMNVLKVNVKGTTWVTKAV 180

Query: 181 LPKMITKNRGAIVNIGSGAAVIVPSHPLYAIYAATKAYVDQLSRSLHVEYKHWGIDVQCQ 240
           LP+MITKNRGAIVNIGSGAAVIVPSHPLYAIYAATKAYVDQLSRSLHVEYKHWGIDVQCQ
Sbjct: 181 LPRMITKNRGAIVNIGSGAAVIVPSHPLYAIYAATKAYVDQLSRSLHVEYKHWGIDVQCQ 240

Query: 241 VPLYVATEMASRVASVSGASLFIPSADDYVSAAIHRIGYEPRCTPYWAHSLQWCFARLLP 300
           VPLYVATEMASRVASVS ASLFIPSADDYVSAAIHRIGYEPRCTPYWAHSLQWCFARLLP
Sbjct: 241 VPLYVATEMASRVASVSRASLFIPSADDYVSAAIHRIGYEPRCTPYWAHSLQWCFARLLP 300

Query: 301 EAALDAWRLSIGLHRRSKGLDAMKGIEGYKQE 333
           EAALD WRLSIGLHRR KGL+AMKGIEGYKQE
Sbjct: 301 EAALDMWRLSIGLHRRRKGLEAMKGIEGYKQE 332

BLAST of Carg14292 vs. NCBI nr
Match: XP_022957233.1 (very-long-chain 3-oxoacyl-CoA reductase-like protein At1g24470 [Cucurbita moschata])

HSP 1 Score: 632.9 bits (1631), Expect = 1.6e-177
Identity = 316/320 (98.75%), Postives = 319/320 (99.69%), Query Frame = 0

Query: 1   MHTMRETFFLLLSTQPIWLLLLSLLGFFTILRHSTTVVQWVFRVFLRPSKDLRYYGSWGV 60
           MHTMRETFFLLLSTQPIWLLLLSLLGFFTILRHSTTVVQWVFRVFLRPSKDLRYYGSWGV
Sbjct: 1   MHTMRETFFLLLSTQPIWLLLLSLLGFFTILRHSTTVVQWVFRVFLRPSKDLRYYGSWGV 60

Query: 61  VTGATDGIGKSFAYQLARAGLNLILVSRSSTKLKAVCKDIRSQFPNTKVKIIELDFTEDD 120
           VTGATDGIGKSFAYQLARAGLNL+LVSRSSTKLKAV KDIRSQFPNTKVKIIELDFTEDD
Sbjct: 61  VTGATDGIGKSFAYQLARAGLNLVLVSRSSTKLKAVSKDIRSQFPNTKVKIIELDFTEDD 120

Query: 121 ISAGMAEFKKVIQDLDLGVLINNVGITYPNASFFHEVDEKVWMNVLKVNVKGTTWVTKAV 180
           ISAGMAEFKKVIQDLD+GVLINNVGITYPNASFFHEVDEKVWMNVLKVNVKGTTWVTKAV
Sbjct: 121 ISAGMAEFKKVIQDLDVGVLINNVGITYPNASFFHEVDEKVWMNVLKVNVKGTTWVTKAV 180

Query: 181 LPKMITKNRGAIVNIGSGAAVIVPSHPLYAIYAATKAYVDQLSRSLHVEYKHWGIDVQCQ 240
           LPKMITKNRGAIVNIGSGAAVIVPSHPLYAIYAATKAYVDQLSRSLHVEYKHWGIDVQCQ
Sbjct: 181 LPKMITKNRGAIVNIGSGAAVIVPSHPLYAIYAATKAYVDQLSRSLHVEYKHWGIDVQCQ 240

Query: 241 VPLYVATEMASRVASVSGASLFIPSADDYVSAAIHRIGYEPRCTPYWAHSLQWCFARLLP 300
           VPLYVATEMASRVASVSGASLFIPSADDYVSAAIHRIGYEPRCTPYWAHSLQWCFARLLP
Sbjct: 241 VPLYVATEMASRVASVSGASLFIPSADDYVSAAIHRIGYEPRCTPYWAHSLQWCFARLLP 300

Query: 301 EAALDAWRLSIGLHRRSKGL 321
           EAALDAWRLSIGLHRRSKG+
Sbjct: 301 EAALDAWRLSIGLHRRSKGI 320

BLAST of Carg14292 vs. NCBI nr
Match: XP_038892142.1 (very-long-chain 3-oxoacyl-CoA reductase-like protein At1g24470 [Benincasa hispida])

HSP 1 Score: 592.4 bits (1526), Expect = 2.4e-165
Identity = 295/332 (88.86%), Postives = 314/332 (94.58%), Query Frame = 0

Query: 1   MHTMRETFFLLLSTQPIWLLLLSLLGFFTILRHSTTVVQWVFRVFLRPSKDLRYYGSWGV 60
           MH M+ETFFL+LSTQP+WLL+LSLLGFFTILRHS+TVVQWVFR FLRPSKDLRYYGSWGV
Sbjct: 1   MHKMKETFFLVLSTQPVWLLILSLLGFFTILRHSSTVVQWVFRAFLRPSKDLRYYGSWGV 60

Query: 61  VTGATDGIGKSFAYQLARAGLNLILVSRSSTKLKAVCKDIRSQFPNTKVKIIELDFTEDD 120
           +TGATDGIGKSFAYQLARAGLN+ILVSRSSTKLKAV KDI+S+FP+TKVKIIELDFT+DD
Sbjct: 61  ITGATDGIGKSFAYQLARAGLNVILVSRSSTKLKAVSKDIQSEFPDTKVKIIELDFTDDD 120

Query: 121 ISAGMAEFKKVIQDLDLGVLINNVGITYPNASFFHEVDEKVWMNVLKVNVKGTTWVTKAV 180
           ISAG+AE K+VI+DLD+G+LINNVGITYPNASFFHEVDEKVWMNV KVNVKGTTWVTKAV
Sbjct: 121 ISAGIAEIKEVIEDLDVGILINNVGITYPNASFFHEVDEKVWMNVFKVNVKGTTWVTKAV 180

Query: 181 LPKMITKNRGAIVNIGSGAAVIVPSHPLYAIYAATKAYVDQLSRSLHVEYKHWGIDVQCQ 240
           LPKMI KN+GAIVNIGSGAAVIVPSHPLYAIYAATKAYVDQLSRSLHVEYK WGIDVQCQ
Sbjct: 181 LPKMIKKNKGAIVNIGSGAAVIVPSHPLYAIYAATKAYVDQLSRSLHVEYKDWGIDVQCQ 240

Query: 241 VPLYVATEMASRVASVSGASLFIPSADDYVSAAIHRIGYEPRCTPYWAHSLQWCFARLLP 300
           VPLYVATEMASRVASVS ASLFIPSADDYV A+I RIGYEPRCTPYWAHSLQWCFA LLP
Sbjct: 241 VPLYVATEMASRVASVSRASLFIPSADDYVKASIRRIGYEPRCTPYWAHSLQWCFACLLP 300

Query: 301 EAALDAWRLSIGLHRRSKGLDAMKGIEGYKQE 333
           EA LDAWRLSIGL RR  G DAMKGIE  KQ+
Sbjct: 301 EATLDAWRLSIGLQRR--GSDAMKGIEDCKQD 330

BLAST of Carg14292 vs. ExPASy Swiss-Prot
Match: Q9FYL6 (Very-long-chain 3-oxoacyl-CoA reductase-like protein At1g24470 OS=Arabidopsis thaliana OX=3702 GN=KCR2 PE=2 SV=1)

HSP 1 Score: 345.5 bits (885), Expect = 6.6e-94
Identity = 170/305 (55.74%), Postives = 227/305 (74.43%), Query Frame = 0

Query: 14  TQPIWLLLLSLLGFFTILR-HSTTVVQWVFRVFLRPSKDLRYYGSWGVVTGATDGIGKSF 73
           +QP +L  +  +GF  +LR     +++W    FL   K L+ YGSW +VTGAT+GIG++F
Sbjct: 9   SQPWYLHFVCFIGFLFLLRVLFIPLLKWFTTRFLLTPKRLKRYGSWAMVTGATEGIGRAF 68

Query: 74  AYQLARAGLNLILVSRSSTKLKAVCKDIRSQFPNTKVKIIELDFTEDDISAGMAEFKKVI 133
           A++LA+ GLNLILVSR+ +KL++V  D + +FP+ K+KII  DF+ +    G    ++ I
Sbjct: 69  AHELAKHGLNLILVSRNLSKLESVSDDFQQEFPHIKIKIIPFDFSSE---GGYGAIEEGI 128

Query: 134 QDLDLGVLINNVGITYPNASFFHEVDEKVWMNVLKVNVKGTTWVTKAVLPKMITKNRGAI 193
           + L++G+LINNVGITYP A FFHEVD+  W  +L+VN++ TTWVT++++  M+ + RGAI
Sbjct: 129 KGLEVGILINNVGITYPRAMFFHEVDQLTWTKILRVNLEATTWVTRSLIGPMLHRRRGAI 188

Query: 194 VNIGSGAAVIVPSHPLYAIYAATKAYVDQLSRSLHVEYKHWGIDVQCQVPLYVATEMASR 253
           VNI SGAAV+VPSHPLYAIYAATKAYVD LSRSLHVEYK +GIDVQCQVPLYV+T M S 
Sbjct: 189 VNISSGAAVVVPSHPLYAIYAATKAYVDALSRSLHVEYKQFGIDVQCQVPLYVSTRMVSE 248

Query: 254 VASVSGASLFIPSADDYVSAAIHRIGYEPRCTPYWAHSLQWCFARLLPEAALDAWRLSIG 313
           VA++   SLF+PS + Y  AA+ +IG   RC+P+WAHSLQW    L+P+  +D WRLSIG
Sbjct: 249 VAAIDKPSLFVPSPEVYAKAAVAQIGIGSRCSPFWAHSLQWFLVGLVPDNLVDTWRLSIG 308

Query: 314 LHRRS 318
           L RRS
Sbjct: 309 LRRRS 310

BLAST of Carg14292 vs. ExPASy Swiss-Prot
Match: Q8L9C4 (Very-long-chain 3-oxoacyl-CoA reductase 1 OS=Arabidopsis thaliana OX=3702 GN=KCR1 PE=1 SV=1)

HSP 1 Score: 318.9 bits (816), Expect = 6.6e-86
Identity = 157/313 (50.16%), Postives = 222/313 (70.93%), Query Frame = 0

Query: 14  TQPIWLLLLSLLGFFTILRHSTTVVQWVFRVFLRPSKDLRYYGSWGVVTGATDGIGKSFA 73
           +QP WLL+L +LG  +I +   T+++  +  FLRPSK+LR YGSW ++TG TDGIGK+FA
Sbjct: 9   SQPTWLLILFVLGSISIFKFIFTLLRSFYIYFLRPSKNLRRYGSWAIITGPTDGIGKAFA 68

Query: 74  YQLARAGLNLILVSRSSTKLKAVCKDIRSQFPNTKVKIIELDFTEDDISAGMAEFKKVIQ 133
           +QLA+ GLNLILV+R+  KLK V   IRS++  T++  + +DF+  DI  G+   K+ I+
Sbjct: 69  FQLAQKGLNLILVARNPDKLKDVSDSIRSKYSQTQILTVVMDFS-GDIDEGVKRIKESIE 128

Query: 134 DLDLGVLINNVGITYPNASFFHEVDEKVWMNVLKVNVKGTTWVTKAVLPKMITKNRGAIV 193
            LD+G+LINN G++YP A +FHEVDE++  N++K+NV+GTT VT+AVLP M+ + +GAI+
Sbjct: 129 GLDVGILINNAGMSYPYAKYFHEVDEELINNLIKINVEGTTKVTQAVLPNMLKRKKGAII 188

Query: 194 NIGSGAAVIVPSHPLYAIYAATKAYVDQLSRSLHVEYKHWGIDVQCQVPLYVATEMASRV 253
           N+GSGAA ++PS+P Y++YA  K YVDQ ++ LHVEYK  GIDVQCQVPLYVAT+M    
Sbjct: 189 NMGSGAAALIPSYPFYSVYAGAKTYVDQFTKCLHVEYKKSGIDVQCQVPLYVATKM---- 248

Query: 254 ASVSGASLFIPSADDYVSAAIHRIGYEPRCTPYWAHSLQWCFARLLPEAALDAWRLSIGL 313
             +  AS  + S + Y  AA+  +GYE +CTPYW H+L       LPE+  +++ +   L
Sbjct: 249 TKIRRASFLVASPEGYAKAALRFVGYEAQCTPYWPHALMGAVVSALPESVFESFNIKRCL 308

Query: 314 HRRSKGL--DAMK 325
             R KGL  D+MK
Sbjct: 309 QIRKKGLQKDSMK 316

BLAST of Carg14292 vs. ExPASy Swiss-Prot
Match: A5PJF6 (Inactive hydroxysteroid dehydrogenase-like protein 1 OS=Bos taurus OX=9913 GN=HSDL1 PE=2 SV=1)

HSP 1 Score: 188.3 bits (477), Expect = 1.4e-46
Identity = 108/292 (36.99%), Postives = 166/292 (56.85%), Query Frame = 0

Query: 22  LSLLGFFTILRHSTTVV---------QWVFRVFLRPSKDLRYYGSWGVVTGATDGIGKSF 81
           L+L+G +   R S TVV          ++ R+  R    ++ YG W VV+GATDGIG+++
Sbjct: 25  LALVGAWYTARKSITVVCDFYSLIRLHFIPRLVSRADL-IKQYGRWAVVSGATDGIGRAY 84

Query: 82  AYQLARAGLNLILVSRSSTKLKAVCKDIRSQFPNTKVKIIELDFTEDDISAGMAEF---K 141
           A +LA  GLN++L+SRS  KL+ V KDI   +       +E D    D S+G   +   +
Sbjct: 85  AEELASRGLNIVLISRSQEKLQMVAKDIADTYK------VETDIIVADFSSGREIYDMIR 144

Query: 142 KVIQDLDLGVLINNVGITYPNASFFHEVDEKVWMNVLKVNVKGTTWVTKAVLPKMITKNR 201
           + +QD D+G+L+NNVG+ YP   +F +V E    +++ VN+   + +   VLP M+ + +
Sbjct: 145 EALQDRDIGILVNNVGVFYPYPQYFTQVSEDTLWDIVNVNIAAASLMVHIVLPGMVERKK 204

Query: 202 GAIVNIGSGAAVIVPSHPLYAIYAATKAYVDQLSRSLHVEYKHWGIDVQCQVPLYVATEM 261
           GAIV I SG+       P  A ++A+KAY+D  SR+L  EY   GI VQ  +P YVAT +
Sbjct: 205 GAIVTISSGSC--CKPTPQLAAFSASKAYLDHFSRALQYEYASKGIFVQSLIPFYVATNV 264

Query: 262 ASRVASVSGASLFIPSADDYVSAAIHRIGYEPRCTPYWAHSLQWCFARLLPE 302
           A+  + +      +PS   Y   A+  +G   R T YW+HS+Q+ FA+ +PE
Sbjct: 265 ATPGSFLHKCPWLVPSPKVYAHHAVSTLGISKRTTGYWSHSIQFLFAQYMPE 307

BLAST of Carg14292 vs. ExPASy Swiss-Prot
Match: Q3SXM5 (Inactive hydroxysteroid dehydrogenase-like protein 1 OS=Homo sapiens OX=9606 GN=HSDL1 PE=1 SV=3)

HSP 1 Score: 186.4 bits (472), Expect = 5.1e-46
Identity = 107/291 (36.77%), Postives = 165/291 (56.70%), Query Frame = 0

Query: 22  LSLLGFFTILRHSTTVV---QWVFRVFLRP-----SKDLRYYGSWGVVTGATDGIGKSFA 81
           L+L+G +   R S TV+     + R+   P     +  ++ YG W VV+GATDGIGK++A
Sbjct: 25  LALVGAWYTARKSITVICDFYSLIRLHFIPRLGSRADLIKQYGRWAVVSGATDGIGKAYA 84

Query: 82  YQLARAGLNLILVSRSSTKLKAVCKDIRSQFPNTKVKIIELDFTEDDISAGMAEF---KK 141
            +LA  GLN+IL+SR+  KL+ V KDI   +       +E D    D S+G   +   ++
Sbjct: 85  EELASRGLNIILISRNEEKLQVVAKDIADTYK------VETDIIVADFSSGREIYLPIRE 144

Query: 142 VIQDLDLGVLINNVGITYPNASFFHEVDEKVWMNVLKVNVKGTTWVTKAVLPKMITKNRG 201
            ++D D+G+L+NNVG+ YP   +F ++ E    +++ VN+   + +   VLP M+ + +G
Sbjct: 145 ALKDKDVGILVNNVGVFYPYPQYFTQLSEDKLWDIINVNIAAASLMVHVVLPGMVERKKG 204

Query: 202 AIVNIGSGAAVIVPSHPLYAIYAATKAYVDQLSRSLHVEYKHWGIDVQCQVPLYVATEMA 261
           AIV I SG+       P  A ++A+KAY+D  SR+L  EY   GI VQ  +P YVAT M 
Sbjct: 205 AIVTISSGSC--CKPTPQLAAFSASKAYLDHFSRALQYEYASKGIFVQSLIPFYVATSMT 264

Query: 262 SRVASVSGASLFIPSADDYVSAAIHRIGYEPRCTPYWAHSLQWCFARLLPE 302
           +    +   S  +PS   Y   A+  +G   R T YW+HS+Q+ FA+ +PE
Sbjct: 265 APSNFLHRCSWLVPSPKVYAHHAVSTLGISKRTTGYWSHSIQFLFAQYMPE 307

BLAST of Carg14292 vs. ExPASy Swiss-Prot
Match: Q5ZJG8 (Hydroxysteroid dehydrogenase-like protein 1 OS=Gallus gallus OX=9031 GN=HSDL1 PE=2 SV=1)

HSP 1 Score: 184.1 bits (466), Expect = 2.6e-45
Identity = 100/253 (39.53%), Postives = 148/253 (58.50%), Query Frame = 0

Query: 52  LRYYGSWGVVTGATDGIGKSFAYQLARAGLNLILVSRSSTKLKAVCKDIRSQFPNTKVKI 111
           ++ YG W VVTG+TDGIGK++A +LA+ G+N+IL+SRS  KL+AV + I   +       
Sbjct: 63  VKRYGRWAVVTGSTDGIGKAYAEELAKRGVNIILISRSKEKLEAVSRSISETYK------ 122

Query: 112 IELDFTEDDISAGMAEF---KKVIQDLDLGVLINNVGITYPNASFFHEVDEKVWMNVLKV 171
           +E DF   D S G   +   K+ ++D ++G+L+NNVG+ Y    +F  + E +  +++ V
Sbjct: 123 VETDFIVADFSKGREAYQAIKEGLKDREIGILVNNVGLFYTYPDYFTNLSEDMLWDMINV 182

Query: 172 NVKGTTWVTKAVLPKMITKNRGAIVNIGSGAAVIVPSHPLYAIYAATKAYVDQLSRSLHV 231
           N+     +   VLP M+ K +GAIVN+ S A+   P+ P+   Y A+KAY+D  SR+L+ 
Sbjct: 183 NIASANMMVHIVLPGMVEKRKGAIVNV-SSASCCQPT-PMLTTYGASKAYLDYFSRALYY 242

Query: 232 EYKHWGIDVQCQVPLYVATEMASRVASVSGASLFIPSADDYVSAAIHRIGYEPRCTPYWA 291
           EY   GI VQ   P  +AT M S     S  S F PSA++Y S AI  +G   R   YW 
Sbjct: 243 EYASKGIFVQSLTPFVIATRMVSCSRVTSKRSFFFPSAEEYASHAISTLGLSKRTPGYWK 302

Query: 292 HSLQWCFARLLPE 302
           HS+++     LPE
Sbjct: 303 HSIEFTLGERLPE 307

BLAST of Carg14292 vs. ExPASy TrEMBL
Match: A0A6J1KC16 (very-long-chain 3-oxoacyl-CoA reductase-like protein At1g24470 OS=Cucurbita maxima OX=3661 GN=LOC111492945 PE=3 SV=1)

HSP 1 Score: 646.4 bits (1666), Expect = 6.7e-182
Identity = 323/332 (97.29%), Postives = 327/332 (98.49%), Query Frame = 0

Query: 1   MHTMRETFFLLLSTQPIWLLLLSLLGFFTILRHSTTVVQWVFRVFLRPSKDLRYYGSWGV 60
           MHTMRETFFLLLSTQPIWLLLLSLLGFFTILRHSTT VQWVFRVFLRPSKDLRYYGSWGV
Sbjct: 1   MHTMRETFFLLLSTQPIWLLLLSLLGFFTILRHSTTAVQWVFRVFLRPSKDLRYYGSWGV 60

Query: 61  VTGATDGIGKSFAYQLARAGLNLILVSRSSTKLKAVCKDIRSQFPNTKVKIIELDFTEDD 120
           VTGATDGIGKSF+YQLARAGLNLILVSRSSTKLKAV KDIRSQFPNTKVKIIELDFTEDD
Sbjct: 61  VTGATDGIGKSFSYQLARAGLNLILVSRSSTKLKAVSKDIRSQFPNTKVKIIELDFTEDD 120

Query: 121 ISAGMAEFKKVIQDLDLGVLINNVGITYPNASFFHEVDEKVWMNVLKVNVKGTTWVTKAV 180
           ISAGMAEFKKVIQDLD+GVLINNVGITYPNASFFHEVDEKVWMNVLKVNVKGTTWVTKAV
Sbjct: 121 ISAGMAEFKKVIQDLDVGVLINNVGITYPNASFFHEVDEKVWMNVLKVNVKGTTWVTKAV 180

Query: 181 LPKMITKNRGAIVNIGSGAAVIVPSHPLYAIYAATKAYVDQLSRSLHVEYKHWGIDVQCQ 240
           LP+MITKNRGAIVNIGSGAAVIVPSHPLYAIYAATKAYVDQLSRSLHVEYKHWGIDVQCQ
Sbjct: 181 LPRMITKNRGAIVNIGSGAAVIVPSHPLYAIYAATKAYVDQLSRSLHVEYKHWGIDVQCQ 240

Query: 241 VPLYVATEMASRVASVSGASLFIPSADDYVSAAIHRIGYEPRCTPYWAHSLQWCFARLLP 300
           VPLYVATEMASRVASVS ASLFIPSADDYVSAAIHRIGYEPRCTPYWAHSLQWCFARLLP
Sbjct: 241 VPLYVATEMASRVASVSRASLFIPSADDYVSAAIHRIGYEPRCTPYWAHSLQWCFARLLP 300

Query: 301 EAALDAWRLSIGLHRRSKGLDAMKGIEGYKQE 333
           EAALD WRLSIGLHRR KGL+AMKGIEGYKQE
Sbjct: 301 EAALDMWRLSIGLHRRRKGLEAMKGIEGYKQE 332

BLAST of Carg14292 vs. ExPASy TrEMBL
Match: A0A6J1GZN0 (very-long-chain 3-oxoacyl-CoA reductase-like protein At1g24470 OS=Cucurbita moschata OX=3662 GN=LOC111458680 PE=3 SV=1)

HSP 1 Score: 632.9 bits (1631), Expect = 7.7e-178
Identity = 316/320 (98.75%), Postives = 319/320 (99.69%), Query Frame = 0

Query: 1   MHTMRETFFLLLSTQPIWLLLLSLLGFFTILRHSTTVVQWVFRVFLRPSKDLRYYGSWGV 60
           MHTMRETFFLLLSTQPIWLLLLSLLGFFTILRHSTTVVQWVFRVFLRPSKDLRYYGSWGV
Sbjct: 1   MHTMRETFFLLLSTQPIWLLLLSLLGFFTILRHSTTVVQWVFRVFLRPSKDLRYYGSWGV 60

Query: 61  VTGATDGIGKSFAYQLARAGLNLILVSRSSTKLKAVCKDIRSQFPNTKVKIIELDFTEDD 120
           VTGATDGIGKSFAYQLARAGLNL+LVSRSSTKLKAV KDIRSQFPNTKVKIIELDFTEDD
Sbjct: 61  VTGATDGIGKSFAYQLARAGLNLVLVSRSSTKLKAVSKDIRSQFPNTKVKIIELDFTEDD 120

Query: 121 ISAGMAEFKKVIQDLDLGVLINNVGITYPNASFFHEVDEKVWMNVLKVNVKGTTWVTKAV 180
           ISAGMAEFKKVIQDLD+GVLINNVGITYPNASFFHEVDEKVWMNVLKVNVKGTTWVTKAV
Sbjct: 121 ISAGMAEFKKVIQDLDVGVLINNVGITYPNASFFHEVDEKVWMNVLKVNVKGTTWVTKAV 180

Query: 181 LPKMITKNRGAIVNIGSGAAVIVPSHPLYAIYAATKAYVDQLSRSLHVEYKHWGIDVQCQ 240
           LPKMITKNRGAIVNIGSGAAVIVPSHPLYAIYAATKAYVDQLSRSLHVEYKHWGIDVQCQ
Sbjct: 181 LPKMITKNRGAIVNIGSGAAVIVPSHPLYAIYAATKAYVDQLSRSLHVEYKHWGIDVQCQ 240

Query: 241 VPLYVATEMASRVASVSGASLFIPSADDYVSAAIHRIGYEPRCTPYWAHSLQWCFARLLP 300
           VPLYVATEMASRVASVSGASLFIPSADDYVSAAIHRIGYEPRCTPYWAHSLQWCFARLLP
Sbjct: 241 VPLYVATEMASRVASVSGASLFIPSADDYVSAAIHRIGYEPRCTPYWAHSLQWCFARLLP 300

Query: 301 EAALDAWRLSIGLHRRSKGL 321
           EAALDAWRLSIGLHRRSKG+
Sbjct: 301 EAALDAWRLSIGLHRRSKGI 320

BLAST of Carg14292 vs. ExPASy TrEMBL
Match: A0A5D3CW53 (Very-long-chain 3-oxoacyl-CoA reductase-like protein OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold35G001930 PE=3 SV=1)

HSP 1 Score: 589.7 bits (1519), Expect = 7.5e-165
Identity = 292/332 (87.95%), Postives = 311/332 (93.67%), Query Frame = 0

Query: 1   MHTMRETFFLLLSTQPIWLLLLSLLGFFTILRHSTTVVQWVFRVFLRPSKDLRYYGSWGV 60
           MH M+ETFFLLLST PIWLL+LSLLGFFTILRHS TV +WVFR FLRPSKDLRYYGSWG+
Sbjct: 1   MHRMKETFFLLLSTNPIWLLILSLLGFFTILRHSATVARWVFRAFLRPSKDLRYYGSWGI 60

Query: 61  VTGATDGIGKSFAYQLARAGLNLILVSRSSTKLKAVCKDIRSQFPNTKVKIIELDFTEDD 120
           VTGATDGIGKSFAYQLARAGLNLILVSRSSTKLKAV +DI+S+FP+TKVKIIELDFTEDD
Sbjct: 61  VTGATDGIGKSFAYQLARAGLNLILVSRSSTKLKAVSEDIQSEFPDTKVKIIELDFTEDD 120

Query: 121 ISAGMAEFKKVIQDLDLGVLINNVGITYPNASFFHEVDEKVWMNVLKVNVKGTTWVTKAV 180
           IS G+AE ++VI+DLD+G+LINNVGITYPNASFFHEVDEKVWMN+ KVNVKGTTWVTKAV
Sbjct: 121 ISGGIAEIEEVIEDLDVGILINNVGITYPNASFFHEVDEKVWMNLFKVNVKGTTWVTKAV 180

Query: 181 LPKMITKNRGAIVNIGSGAAVIVPSHPLYAIYAATKAYVDQLSRSLHVEYKHWGIDVQCQ 240
           LPKMI KNRGAIVNIGSGAAVIVPSHPLYAIYAATKAYVDQLSRSL+VEYK WGIDVQCQ
Sbjct: 181 LPKMIKKNRGAIVNIGSGAAVIVPSHPLYAIYAATKAYVDQLSRSLYVEYKDWGIDVQCQ 240

Query: 241 VPLYVATEMASRVASVSGASLFIPSADDYVSAAIHRIGYEPRCTPYWAHSLQWCFARLLP 300
           VPLYVATEMASRVASVS ASLF+PSADDYV AAI +IGYEPRCTPYWAHSLQWCFARLLP
Sbjct: 241 VPLYVATEMASRVASVSRASLFVPSADDYVKAAIRQIGYEPRCTPYWAHSLQWCFARLLP 300

Query: 301 EAALDAWRLSIGLHRRSKGLDAMKGIEGYKQE 333
           EA LDAWRLSIGL RRS G  AM+G EG KQE
Sbjct: 301 EAVLDAWRLSIGLQRRSNGSVAMEGTEGCKQE 332

BLAST of Carg14292 vs. ExPASy TrEMBL
Match: A0A1S3BE86 (very-long-chain 3-oxoacyl-CoA reductase-like protein At1g24470 OS=Cucumis melo OX=3656 GN=LOC103488924 PE=3 SV=1)

HSP 1 Score: 589.7 bits (1519), Expect = 7.5e-165
Identity = 292/332 (87.95%), Postives = 311/332 (93.67%), Query Frame = 0

Query: 1   MHTMRETFFLLLSTQPIWLLLLSLLGFFTILRHSTTVVQWVFRVFLRPSKDLRYYGSWGV 60
           MH M+ETFFLLLST PIWLL+LSLLGFFTILRHS TV +WVFR FLRPSKDLRYYGSWG+
Sbjct: 1   MHRMKETFFLLLSTNPIWLLILSLLGFFTILRHSATVARWVFRAFLRPSKDLRYYGSWGI 60

Query: 61  VTGATDGIGKSFAYQLARAGLNLILVSRSSTKLKAVCKDIRSQFPNTKVKIIELDFTEDD 120
           VTGATDGIGKSFAYQLARAGLNLILVSRSSTKLKAV +DI+S+FP+TKVKIIELDFTEDD
Sbjct: 61  VTGATDGIGKSFAYQLARAGLNLILVSRSSTKLKAVSEDIQSEFPDTKVKIIELDFTEDD 120

Query: 121 ISAGMAEFKKVIQDLDLGVLINNVGITYPNASFFHEVDEKVWMNVLKVNVKGTTWVTKAV 180
           IS G+AE ++VI+DLD+G+LINNVGITYPNASFFHEVDEKVWMN+ KVNVKGTTWVTKAV
Sbjct: 121 ISGGIAEIEEVIEDLDVGILINNVGITYPNASFFHEVDEKVWMNLFKVNVKGTTWVTKAV 180

Query: 181 LPKMITKNRGAIVNIGSGAAVIVPSHPLYAIYAATKAYVDQLSRSLHVEYKHWGIDVQCQ 240
           LPKMI KNRGAIVNIGSGAAVIVPSHPLYAIYAATKAYVDQLSRSL+VEYK WGIDVQCQ
Sbjct: 181 LPKMIKKNRGAIVNIGSGAAVIVPSHPLYAIYAATKAYVDQLSRSLYVEYKDWGIDVQCQ 240

Query: 241 VPLYVATEMASRVASVSGASLFIPSADDYVSAAIHRIGYEPRCTPYWAHSLQWCFARLLP 300
           VPLYVATEMASRVASVS ASLF+PSADDYV AAI +IGYEPRCTPYWAHSLQWCFARLLP
Sbjct: 241 VPLYVATEMASRVASVSRASLFVPSADDYVKAAIRQIGYEPRCTPYWAHSLQWCFARLLP 300

Query: 301 EAALDAWRLSIGLHRRSKGLDAMKGIEGYKQE 333
           EA LDAWRLSIGL RRS G  AM+G EG KQE
Sbjct: 301 EAVLDAWRLSIGLQRRSNGSVAMEGTEGCKQE 332

BLAST of Carg14292 vs. ExPASy TrEMBL
Match: A0A0A0KS33 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_5G591790 PE=3 SV=1)

HSP 1 Score: 582.0 bits (1499), Expect = 1.6e-162
Identity = 290/332 (87.35%), Postives = 306/332 (92.17%), Query Frame = 0

Query: 1   MHTMRETFFLLLSTQPIWLLLLSLLGFFTILRHSTTVVQWVFRVFLRPSKDLRYYGSWGV 60
           MHTM+ETFFLLLST PIWLL+LS LGFFTIL HS TV +WV+R FLRPSKDLRYYGSWG+
Sbjct: 1   MHTMKETFFLLLSTSPIWLLILSFLGFFTILWHSATVARWVYRAFLRPSKDLRYYGSWGI 60

Query: 61  VTGATDGIGKSFAYQLARAGLNLILVSRSSTKLKAVCKDIRSQFPNTKVKIIELDFTEDD 120
           VTGATDGIGKSFAYQLARAGLNL+LVSRSS KLKAV KDI+S+FP+TKVKIIELDFTEDD
Sbjct: 61  VTGATDGIGKSFAYQLARAGLNLVLVSRSSMKLKAVSKDIQSEFPDTKVKIIELDFTEDD 120

Query: 121 ISAGMAEFKKVIQDLDLGVLINNVGITYPNASFFHEVDEKVWMNVLKVNVKGTTWVTKAV 180
           IS G+AE ++VI+DLD+G+LINNVGITYPNASFFHEVDEKVWMNV KVNVKGTTWVTKAV
Sbjct: 121 ISGGIAEIEEVIEDLDVGILINNVGITYPNASFFHEVDEKVWMNVFKVNVKGTTWVTKAV 180

Query: 181 LPKMITKNRGAIVNIGSGAAVIVPSHPLYAIYAATKAYVDQLSRSLHVEYKHWGIDVQCQ 240
           LPKMI KNRGAIVNIGSGAAVIVPSHPLYAIYAATKAYVDQLSRSLHVEYK WGIDVQCQ
Sbjct: 181 LPKMIKKNRGAIVNIGSGAAVIVPSHPLYAIYAATKAYVDQLSRSLHVEYKDWGIDVQCQ 240

Query: 241 VPLYVATEMASRVASVSGASLFIPSADDYVSAAIHRIGYEPRCTPYWAHSLQWCFARLLP 300
           VPLYVATEMASRVASVS ASLFIPSADDYV AAI +IGYEPRCTPYWAHSLQWCFA LLP
Sbjct: 241 VPLYVATEMASRVASVSQASLFIPSADDYVKAAIRQIGYEPRCTPYWAHSLQWCFASLLP 300

Query: 301 EAALDAWRLSIGLHRRSKGLDAMKGIEGYKQE 333
           EA LDAWRLSIGL RR K    MK IEG KQE
Sbjct: 301 EAMLDAWRLSIGLERRRKESVTMKRIEGCKQE 332

BLAST of Carg14292 vs. TAIR 10
Match: AT1G24470.1 (beta-ketoacyl reductase 2 )

HSP 1 Score: 345.5 bits (885), Expect = 4.7e-95
Identity = 170/305 (55.74%), Postives = 227/305 (74.43%), Query Frame = 0

Query: 14  TQPIWLLLLSLLGFFTILR-HSTTVVQWVFRVFLRPSKDLRYYGSWGVVTGATDGIGKSF 73
           +QP +L  +  +GF  +LR     +++W    FL   K L+ YGSW +VTGAT+GIG++F
Sbjct: 9   SQPWYLHFVCFIGFLFLLRVLFIPLLKWFTTRFLLTPKRLKRYGSWAMVTGATEGIGRAF 68

Query: 74  AYQLARAGLNLILVSRSSTKLKAVCKDIRSQFPNTKVKIIELDFTEDDISAGMAEFKKVI 133
           A++LA+ GLNLILVSR+ +KL++V  D + +FP+ K+KII  DF+ +    G    ++ I
Sbjct: 69  AHELAKHGLNLILVSRNLSKLESVSDDFQQEFPHIKIKIIPFDFSSE---GGYGAIEEGI 128

Query: 134 QDLDLGVLINNVGITYPNASFFHEVDEKVWMNVLKVNVKGTTWVTKAVLPKMITKNRGAI 193
           + L++G+LINNVGITYP A FFHEVD+  W  +L+VN++ TTWVT++++  M+ + RGAI
Sbjct: 129 KGLEVGILINNVGITYPRAMFFHEVDQLTWTKILRVNLEATTWVTRSLIGPMLHRRRGAI 188

Query: 194 VNIGSGAAVIVPSHPLYAIYAATKAYVDQLSRSLHVEYKHWGIDVQCQVPLYVATEMASR 253
           VNI SGAAV+VPSHPLYAIYAATKAYVD LSRSLHVEYK +GIDVQCQVPLYV+T M S 
Sbjct: 189 VNISSGAAVVVPSHPLYAIYAATKAYVDALSRSLHVEYKQFGIDVQCQVPLYVSTRMVSE 248

Query: 254 VASVSGASLFIPSADDYVSAAIHRIGYEPRCTPYWAHSLQWCFARLLPEAALDAWRLSIG 313
           VA++   SLF+PS + Y  AA+ +IG   RC+P+WAHSLQW    L+P+  +D WRLSIG
Sbjct: 249 VAAIDKPSLFVPSPEVYAKAAVAQIGIGSRCSPFWAHSLQWFLVGLVPDNLVDTWRLSIG 308

Query: 314 LHRRS 318
           L RRS
Sbjct: 309 LRRRS 310

BLAST of Carg14292 vs. TAIR 10
Match: AT1G67730.1 (beta-ketoacyl reductase 1 )

HSP 1 Score: 318.9 bits (816), Expect = 4.7e-87
Identity = 157/313 (50.16%), Postives = 222/313 (70.93%), Query Frame = 0

Query: 14  TQPIWLLLLSLLGFFTILRHSTTVVQWVFRVFLRPSKDLRYYGSWGVVTGATDGIGKSFA 73
           +QP WLL+L +LG  +I +   T+++  +  FLRPSK+LR YGSW ++TG TDGIGK+FA
Sbjct: 9   SQPTWLLILFVLGSISIFKFIFTLLRSFYIYFLRPSKNLRRYGSWAIITGPTDGIGKAFA 68

Query: 74  YQLARAGLNLILVSRSSTKLKAVCKDIRSQFPNTKVKIIELDFTEDDISAGMAEFKKVIQ 133
           +QLA+ GLNLILV+R+  KLK V   IRS++  T++  + +DF+  DI  G+   K+ I+
Sbjct: 69  FQLAQKGLNLILVARNPDKLKDVSDSIRSKYSQTQILTVVMDFS-GDIDEGVKRIKESIE 128

Query: 134 DLDLGVLINNVGITYPNASFFHEVDEKVWMNVLKVNVKGTTWVTKAVLPKMITKNRGAIV 193
            LD+G+LINN G++YP A +FHEVDE++  N++K+NV+GTT VT+AVLP M+ + +GAI+
Sbjct: 129 GLDVGILINNAGMSYPYAKYFHEVDEELINNLIKINVEGTTKVTQAVLPNMLKRKKGAII 188

Query: 194 NIGSGAAVIVPSHPLYAIYAATKAYVDQLSRSLHVEYKHWGIDVQCQVPLYVATEMASRV 253
           N+GSGAA ++PS+P Y++YA  K YVDQ ++ LHVEYK  GIDVQCQVPLYVAT+M    
Sbjct: 189 NMGSGAAALIPSYPFYSVYAGAKTYVDQFTKCLHVEYKKSGIDVQCQVPLYVATKM---- 248

Query: 254 ASVSGASLFIPSADDYVSAAIHRIGYEPRCTPYWAHSLQWCFARLLPEAALDAWRLSIGL 313
             +  AS  + S + Y  AA+  +GYE +CTPYW H+L       LPE+  +++ +   L
Sbjct: 249 TKIRRASFLVASPEGYAKAALRFVGYEAQCTPYWPHALMGAVVSALPESVFESFNIKRCL 308

Query: 314 HRRSKGL--DAMK 325
             R KGL  D+MK
Sbjct: 309 QIRKKGLQKDSMK 316

BLAST of Carg14292 vs. TAIR 10
Match: AT3G55310.1 (NAD(P)-binding Rossmann-fold superfamily protein )

HSP 1 Score: 79.0 bits (193), Expect = 8.2e-15
Identity = 59/178 (33.15%), Postives = 91/178 (51.12%), Query Frame = 0

Query: 60  VVTGATDGIGKSFAYQLARAGLNLILVSRSSTKLKAVCKDIRSQFPNTKVKIIELDFTED 119
           +VTGA+ GIG+     LA+AG  +I  +R   +L ++C +I S F +T ++   L+    
Sbjct: 23  LVTGASSGIGREICLDLAKAGCQVIAAARRVDRLNSLCSEINS-FSSTGIQAAALEL--- 82

Query: 120 DISAGMAEFKKVIQDL-----DLGVLINNVGITYPNASFFHEVDEKVWMNVLKVNVKGTT 179
           D+S+  A  +K +++       +  LINN GI   N     ++ E  W NV   N+KG  
Sbjct: 83  DVSSDAATIQKAVREAWDIFGKIDALINNAGIR-GNVKLSLDLSEDEWDNVFNTNLKGPW 142

Query: 180 WVTKAVLPKM-ITKNRGAIVNIGSGAAV--IVPSHPLYAIYAATKAYVDQLSRSLHVE 230
            V K V   M   K  G+++NI S A V  IVP       Y+ +K  VD +SR + +E
Sbjct: 143 LVAKYVCVLMRDAKRGGSVINISSVAGVRSIVPGG---LAYSCSKGGVDTMSRMMAIE 192

BLAST of Carg14292 vs. TAIR 10
Match: AT3G55290.1 (NAD(P)-binding Rossmann-fold superfamily protein )

HSP 1 Score: 77.8 bits (190), Expect = 1.8e-14
Identity = 59/176 (33.52%), Postives = 91/176 (51.70%), Query Frame = 0

Query: 60  VVTGATDGIGKSFAYQLARAGLNLILVSRSSTKLKAVCKDIRSQFPNTKVKIIELDFTED 119
           +VTGA+ GIG+     LA+AG  +I  +R   +L ++C +I S F +T ++   L+    
Sbjct: 24  LVTGASSGIGREICLDLAKAGCQVIAAARRVDRLNSLCSEINS-FSSTGIQAAALEL--- 83

Query: 120 DISAGMAEFKKVIQDL-----DLGVLINNVGITYPNASFFHEVDEKVWMNVLKVNVKGTT 179
           D+S+  A  +K +++       +  LINN GI   N     ++ E  W NV K N+KG  
Sbjct: 84  DVSSDAATIQKAVREAWDIFGKIDALINNAGIR-GNVKSSLDLSEDEWDNVFKTNLKGPW 143

Query: 180 WVTKAVLPKM-ITKNRGAIVNIGSGAAVIVPSHPLYAIYAATKAYVDQLSRSLHVE 230
            V+K V   M   K  G+++NI S  A I    P    YA +K  VD +SR + +E
Sbjct: 144 LVSKHVCMLMRDAKRGGSVINI-SSIAGIRGMLPGGLAYACSKGGVDTMSRMMALE 193

BLAST of Carg14292 vs. TAIR 10
Match: AT3G55290.2 (NAD(P)-binding Rossmann-fold superfamily protein )

HSP 1 Score: 77.8 bits (190), Expect = 1.8e-14
Identity = 59/176 (33.52%), Postives = 91/176 (51.70%), Query Frame = 0

Query: 60  VVTGATDGIGKSFAYQLARAGLNLILVSRSSTKLKAVCKDIRSQFPNTKVKIIELDFTED 119
           +VTGA+ GIG+     LA+AG  +I  +R   +L ++C +I S F +T ++   L+    
Sbjct: 23  LVTGASSGIGREICLDLAKAGCQVIAAARRVDRLNSLCSEINS-FSSTGIQAAALEL--- 82

Query: 120 DISAGMAEFKKVIQDL-----DLGVLINNVGITYPNASFFHEVDEKVWMNVLKVNVKGTT 179
           D+S+  A  +K +++       +  LINN GI   N     ++ E  W NV K N+KG  
Sbjct: 83  DVSSDAATIQKAVREAWDIFGKIDALINNAGIR-GNVKSSLDLSEDEWDNVFKTNLKGPW 142

Query: 180 WVTKAVLPKM-ITKNRGAIVNIGSGAAVIVPSHPLYAIYAATKAYVDQLSRSLHVE 230
            V+K V   M   K  G+++NI S  A I    P    YA +K  VD +SR + +E
Sbjct: 143 LVSKHVCMLMRDAKRGGSVINI-SSIAGIRGMLPGGLAYACSKGGVDTMSRMMALE 192

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
KAG6601567.18.4e-187100.00Very-long-chain 3-oxoacyl-CoA reductase-like protein, partial [Cucurbita argyros... [more]
XP_023517363.14.3e-18398.49very-long-chain 3-oxoacyl-CoA reductase-like protein At1g24470 [Cucurbita pepo s... [more]
XP_022998255.11.4e-18197.29very-long-chain 3-oxoacyl-CoA reductase-like protein At1g24470 [Cucurbita maxima... [more]
XP_022957233.11.6e-17798.75very-long-chain 3-oxoacyl-CoA reductase-like protein At1g24470 [Cucurbita moscha... [more]
XP_038892142.12.4e-16588.86very-long-chain 3-oxoacyl-CoA reductase-like protein At1g24470 [Benincasa hispid... [more]
Match NameE-valueIdentityDescription
Q9FYL66.6e-9455.74Very-long-chain 3-oxoacyl-CoA reductase-like protein At1g24470 OS=Arabidopsis th... [more]
Q8L9C46.6e-8650.16Very-long-chain 3-oxoacyl-CoA reductase 1 OS=Arabidopsis thaliana OX=3702 GN=KCR... [more]
A5PJF61.4e-4636.99Inactive hydroxysteroid dehydrogenase-like protein 1 OS=Bos taurus OX=9913 GN=HS... [more]
Q3SXM55.1e-4636.77Inactive hydroxysteroid dehydrogenase-like protein 1 OS=Homo sapiens OX=9606 GN=... [more]
Q5ZJG82.6e-4539.53Hydroxysteroid dehydrogenase-like protein 1 OS=Gallus gallus OX=9031 GN=HSDL1 PE... [more]
Match NameE-valueIdentityDescription
A0A6J1KC166.7e-18297.29very-long-chain 3-oxoacyl-CoA reductase-like protein At1g24470 OS=Cucurbita maxi... [more]
A0A6J1GZN07.7e-17898.75very-long-chain 3-oxoacyl-CoA reductase-like protein At1g24470 OS=Cucurbita mosc... [more]
A0A5D3CW537.5e-16587.95Very-long-chain 3-oxoacyl-CoA reductase-like protein OS=Cucumis melo var. makuwa... [more]
A0A1S3BE867.5e-16587.95very-long-chain 3-oxoacyl-CoA reductase-like protein At1g24470 OS=Cucumis melo O... [more]
A0A0A0KS331.6e-16287.35Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_5G591790 PE=3 SV=1[more]
Match NameE-valueIdentityDescription
AT1G24470.14.7e-9555.74beta-ketoacyl reductase 2 [more]
AT1G67730.14.7e-8750.16beta-ketoacyl reductase 1 [more]
AT3G55310.18.2e-1533.15NAD(P)-binding Rossmann-fold superfamily protein [more]
AT3G55290.11.8e-1433.52NAD(P)-binding Rossmann-fold superfamily protein [more]
AT3G55290.21.8e-1433.52NAD(P)-binding Rossmann-fold superfamily protein [more]
InterPro
Analysis Name: InterPro Annotations of Silver-seed gourd (SMH-JMG-627) v2
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR002347Short-chain dehydrogenase/reductase SDRPRINTSPR00080SDRFAMILYcoord: 190..198
score: 54.45
coord: 135..146
score: 41.85
coord: 212..231
score: 33.66
IPR002347Short-chain dehydrogenase/reductase SDRPRINTSPR00081GDHRDHcoord: 212..231
score: 34.59
coord: 135..146
score: 39.64
coord: 233..250
score: 29.78
coord: 58..75
score: 37.51
coord: 184..200
score: 33.74
IPR002347Short-chain dehydrogenase/reductase SDRPFAMPF00106adh_shortcoord: 60..253
e-value: 3.2E-44
score: 150.7
NoneNo IPR availableGENE3D3.40.50.720coord: 53..280
e-value: 8.0E-53
score: 181.3
NoneNo IPR availablePIRSFPIRSF00012611-beta-HSD1coord: 9..322
e-value: 1.5E-94
score: 314.2
NoneNo IPR availablePIRSRPIRSR000095-1PIRSR000095-1coord: 60..258
e-value: 4.2E-18
score: 63.4
NoneNo IPR availablePANTHERPTHR43899RH59310Pcoord: 18..321
NoneNo IPR availablePANTHERPTHR43899:SF26ENOYL-(ACYL CARRIER) REDUCTASEcoord: 18..321
NoneNo IPR availableCDDcd0535617beta-HSD1_like_SDR_ccoord: 56..301
e-value: 6.23164E-97
score: 284.881
IPR020904Short-chain dehydrogenase/reductase, conserved sitePROSITEPS00061ADH_SHORTcoord: 199..227
IPR036291NAD(P)-binding domain superfamilySUPERFAMILY51735NAD(P)-binding Rossmann-fold domainscoord: 54..313

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Carg14292-RACarg14292-RAmRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
cellular_component GO:0005783 endoplasmic reticulum
cellular_component GO:0016021 integral component of membrane
molecular_function GO:0045703 ketoreductase activity
molecular_function GO:0016491 oxidoreductase activity