Carg14258 (gene) Silver-seed gourd (SMH-JMG-627) v2

Overview
NameCarg14258
Typegene
OrganismCucurbita argyrosperma subsp. argyrosperma cv. SMH-JMG-627 (Silver-seed gourd (SMH-JMG-627) v2)
Descriptionreceptor-like cytosolic serine/threonine-protein kinase RBK1
LocationCarg_Chr04: 4658686 .. 4661533 (-)
RNA-Seq ExpressionCarg14258
SyntenyCarg14258
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideexonCDSthree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
TTCTTCCTCCTTCCATATTTTTTCATCTTTCCTTTTGTTCCGCCCGCGGGAGCTTCCGCTTCTGCTCCCTCGAATGTGACTGAGATTCCTTCCCCTTTTCCTTTCATTCTTTCGACTCTCATTTTTTTTTTCTTCTTTTCTACAGGGGAGGGGATTCATCTCTTCTTGTTCTTCCCTTTTCTGTCTCTCATTCACAACTACCACTGCCCAAGGTATGTCCTATTTCATTCCTTTTGCATTGTTCTTCCCACCAACCTCATTTTTGTTTTTGCTTTTGTTTTTTGAATCTATTCTCTACCGATTTTCTGGGATTGGACCACATTAGGTATTCATTTCTTTTGATGTTTTCTTGTATCATTCATCATTTTTGTTATTATTTTTCTTTTCTTCTTAATGCAGGAGATTAAACATTGATCAAATTCTAAAAATGAAAGTGGAAATGGAAATGGAATTTGGTAAATTCTGTTATTACTTTTCTTTTTAGTTAAGCTACAGACGACTTGAATGGAAATGAAATTTTGAATCGATGCATGAAACTCAGGGAATGCTCGAAAAGGAATAAAGCAAATGGAAGAAATGGAAGGATACGGGGGGCTGAAAATCATTGGCGAGGAGGAAGATCTATGCGAAGAAAACTCGCCAAGGGGTGTGTTTGAGATTCCAGTTCTGGGGACGGATTCGGACCAAAGCGAGAGCAGCAGCGGGGGCGACGGAGGGAGCCTGACGGTGGCTGTAAACTCGATGTTGCCGCCGCCGGAGAACTCGCTGTTGCCATCGGCTCTTCTCCAAGAGAGCGGTGGAATTCCATGGAAGGCGATGATCGGGTTGATAAAAAAGAAATCGGTTTGGAGATTGTCGACGATTCCATTACTGGCTATGAGCTACGATATTTCGAAGAAGAATTTCAGCAAAAGATTTGCTCGTATTCGCAGCACTGACGAGGGAATCAGTATCGAGGATGTTCCCTTCTGCAAACCTTCTTGGAGAATTTATGGCTTCTCGGAGCTGGCCACTGCTACCAACAATTTCAGCCCAGGTAAATCACAAATTTGGTCGATTCTTTTTCGACTAAAACAACATTTTGGATCCGGAGATTCCAACTTCTATGTTATGAATAGGTTTAGAATATGTTTCTAAAAAATAAAATCTAATTTACATTTTGTTTAGGTTGTCTTTATGGTTCCAAAATTGCAATTATATGGTTGGTTCACTGTTTCAGAAAACCTGCTTGGAAAAGGAGGGCAAGCAGAGGTTTACAGAGGTTGTTTATCCGATGGCCAATTTGTGGCGGTTAAGAGACTTATGAAAAGGGAGAAGGACAACGAGGAAAAAACTTCTGATTTCTTAACGGAGCTTGGAATTATCGCTCATATTAGCCACCCCAACGCTGCACGGTTGCTTGGTTTTGGAATTGATGGTGGTCTCTACCTTGTCCTCGAATACATCCCTTTTGGCAGCTTTGCCTCTGCCCTTTTCGGTTCGTCACTCTCCCACATCTACGTTACGAAAGTGTTGTGTTGTTAGAATTAATTTGAATGAACAAACAAACCAGGCCGTCTTACTTTCAATCATGGATATATACTTTCATTCAGGTGCGGAATCTCTGGATTGGAAGATAAGATTTAAGGTGGCTATTGGCGTAGCAGACGGGCTGAACTATCTCCATCGTGAATGTCCAAGGCGCATCATTCACAGAGATATCAAAGCCTCTAACATTTTGCTCGGTGAAAACTATGAGCCACTGGTAACGCCTTTGTCTAAAGCATGAAGTTTCAGACACTTCTCTTCCTGTAATAATGAAGATGGCTCTTCTTTCCTTGCAGATCTCTGATTTTGGATTAGCGAAGTGGCTGCCTGAAAATTGGGCTCACCATGTCGTCTACCCCATCGAGGGTACGTTTGGGTAAGTTATACGACTCAGTGGTTCGATTTGGTTTAAAAAAATTATATCAAGACCTATTATCAGGCAGCCCCTAACCTAATGCATCTTTAAATTGCCTCCCTCAGATATTTGGCTCCAGAGTACTTCATGCATGGAATCGTTGATGAGAAGACGGATGTTTTCGCTTATGGGGTTTTGTTGCTTGAGCTCATAACTGGGCGCCGTGCAGTTGATTCAAGCCAACAAAGCCTAGTGATTTGGGTATGAATGATTCAGTGATATCCTTATAGCATCTCTATCTAGTATCTGAAGTTGGTCTTAAATGATTCCTTGCGTACTTAGAATAAAGTTTTTGTTTTGTTTTTGCAGGCAAAGCCTTTACTTGATAACAATAGCTTCCAGGAATTAATGGATCCAAAGTTAGGAGACGACTACGATCAAACTGAAATGAGACGAACCATGTTAGCAGCTTCGATGTGCATAAACCAGTCATCATCGATGCGGCCACATATGAACTCGGTAAGGGTAGCATCCGATTGATATCCATTTATGTTGCGACTTTTCCACTGGCTGACTTGAGGGTTGTGGGAGGGTAGGTAGTGCAGCTGCTGAAGGGAGAAGAAGGAGCATTAGAACAATTAAAACAGAACGCGATGGATGAGGATCAATCGTGCGCTTCGGAAGCCAGCAACATGGAAGATTACTCATGTAATTCAAACTACCTGGACGACATGAGTCGTCACAGGGAGCTGATTTTGGAGTGAAGCGTGATGAATTGAAGTGAAGAAAAGTTGGTTGTTTGCGTTACAAGTTATACAAAATGATGCGTATGCAATTGTGTCAAAGGCTTCTCTGATATAATGTTGACACAAGTGAGTGTCGGTTTCTTCTAAGACTGTGCCTGTGTTTATATGCAACCTTGAAAGGCTCTTCTTTTTGTCAAAAAGTACAGGGATGAAAAAAAAA

mRNA sequence

TTCTTCCTCCTTCCATATTTTTTCATCTTTCCTTTTGTTCCGCCCGCGGGAGCTTCCGCTTCTGCTCCCTCGAATGTGACTGAGATTCCTTCCCCTTTTCCTTTCATTCTTTCGACTCTCATTTTTTTTTTCTTCTTTTCTACAGGGGAGGGGATTCATCTCTTCTTGTTCTTCCCTTTTCTGTCTCTCATTCACAACTACCACTGCCCAAGGAGATTAAACATTGATCAAATTCTAAAAATGAAAGTGGAAATGGAAATGGAATTTGGGAATGCTCGAAAAGGAATAAAGCAAATGGAAGAAATGGAAGGATACGGGGGGCTGAAAATCATTGGCGAGGAGGAAGATCTATGCGAAGAAAACTCGCCAAGGGGTGTGTTTGAGATTCCAGTTCTGGGGACGGATTCGGACCAAAGCGAGAGCAGCAGCGGGGGCGACGGAGGGAGCCTGACGGTGGCTGTAAACTCGATGTTGCCGCCGCCGGAGAACTCGCTGTTGCCATCGGCTCTTCTCCAAGAGAGCGGTGGAATTCCATGGAAGGCGATGATCGGGTTGATAAAAAAGAAATCGGTTTGGAGATTGTCGACGATTCCATTACTGGCTATGAGCTACGATATTTCGAAGAAGAATTTCAGCAAAAGATTTGCTCGTATTCGCAGCACTGACGAGGGAATCAGTATCGAGGATGTTCCCTTCTGCAAACCTTCTTGGAGAATTTATGGCTTCTCGGAGCTGGCCACTGCTACCAACAATTTCAGCCCAGAAAACCTGCTTGGAAAAGGAGGGCAAGCAGAGGTTTACAGAGGTTGTTTATCCGATGGCCAATTTGTGGCGGTTAAGAGACTTATGAAAAGGGAGAAGGACAACGAGGAAAAAACTTCTGATTTCTTAACGGAGCTTGGAATTATCGCTCATATTAGCCACCCCAACGCTGCACGGTTGCTTGGTTTTGGAATTGATGGTGGTCTCTACCTTGTCCTCGAATACATCCCTTTTGGCAGCTTTGCCTCTGCCCTTTTCGGTGCGGAATCTCTGGATTGGAAGATAAGATTTAAGGTGGCTATTGGCGTAGCAGACGGGCTGAACTATCTCCATCGTGAATGTCCAAGGCGCATCATTCACAGAGATATCAAAGCCTCTAACATTTTGCTCGGTGAAAACTATGAGCCACTGATCTCTGATTTTGGATTAGCGAAGTGGCTGCCTGAAAATTGGGCTCACCATGTCGTCTACCCCATCGAGGGTACGTTTGGATATTTGGCTCCAGAGTACTTCATGCATGGAATCGTTGATGAGAAGACGGATGTTTTCGCTTATGGGGTTTTGTTGCTTGAGCTCATAACTGGGCGCCGTGCAGTTGATTCAAGCCAACAAAGCCTAGTGATTTGGGCAAAGCCTTTACTTGATAACAATAGCTTCCAGGAATTAATGGATCCAAAGTTAGGAGACGACTACGATCAAACTGAAATGAGACGAACCATGTTAGCAGCTTCGATGTGCATAAACCAGTCATCATCGATGCGGCCACATATGAACTCGGTAGTGCAGCTGCTGAAGGGAGAAGAAGGAGCATTAGAACAATTAAAACAGAACGCGATGGATGAGGATCAATCGTGCGCTTCGGAAGCCAGCAACATGGAAGATTACTCATGTAATTCAAACTACCTGGACGACATGAGTCGTCACAGGGAGCTGATTTTGGAGTGAAGCGTGATGAATTGAAGTGAAGAAAAGTTGGTTGTTTGCGTTACAAGTTATACAAAATGATGCGTATGCAATTGTGTCAAAGGCTTCTCTGATATAATGTTGACACAAGTGAGTGTCGGTTTCTTCTAAGACTGTGCCTGTGTTTATATGCAACCTTGAAAGGCTCTTCTTTTTGTCAAAAAGTACAGGGATGAAAAAAAAA

Coding sequence (CDS)

TTCTTCCTCCTTCCATATTTTTTCATCTTTCCTTTTGTTCCGCCCGCGGGAGCTTCCGCTTCTGCTCCCTCGAATGTGACTGAGATTCCTTCCCCTTTTCCTTTCATTCTTTCGACTCTCATTTTTTTTTTCTTCTTTTCTACAGGGGAGGGGATTCATCTCTTCTTGTTCTTCCCTTTTCTGTCTCTCATTCACAACTACCACTGCCCAAGGAGATTAAACATTGATCAAATTCTAAAAATGAAAGTGGAAATGGAAATGGAATTTGGGAATGCTCGAAAAGGAATAAAGCAAATGGAAGAAATGGAAGGATACGGGGGGCTGAAAATCATTGGCGAGGAGGAAGATCTATGCGAAGAAAACTCGCCAAGGGGTGTGTTTGAGATTCCAGTTCTGGGGACGGATTCGGACCAAAGCGAGAGCAGCAGCGGGGGCGACGGAGGGAGCCTGACGGTGGCTGTAAACTCGATGTTGCCGCCGCCGGAGAACTCGCTGTTGCCATCGGCTCTTCTCCAAGAGAGCGGTGGAATTCCATGGAAGGCGATGATCGGGTTGATAAAAAAGAAATCGGTTTGGAGATTGTCGACGATTCCATTACTGGCTATGAGCTACGATATTTCGAAGAAGAATTTCAGCAAAAGATTTGCTCGTATTCGCAGCACTGACGAGGGAATCAGTATCGAGGATGTTCCCTTCTGCAAACCTTCTTGGAGAATTTATGGCTTCTCGGAGCTGGCCACTGCTACCAACAATTTCAGCCCAGAAAACCTGCTTGGAAAAGGAGGGCAAGCAGAGGTTTACAGAGGTTGTTTATCCGATGGCCAATTTGTGGCGGTTAAGAGACTTATGAAAAGGGAGAAGGACAACGAGGAAAAAACTTCTGATTTCTTAACGGAGCTTGGAATTATCGCTCATATTAGCCACCCCAACGCTGCACGGTTGCTTGGTTTTGGAATTGATGGTGGTCTCTACCTTGTCCTCGAATACATCCCTTTTGGCAGCTTTGCCTCTGCCCTTTTCGGTGCGGAATCTCTGGATTGGAAGATAAGATTTAAGGTGGCTATTGGCGTAGCAGACGGGCTGAACTATCTCCATCGTGAATGTCCAAGGCGCATCATTCACAGAGATATCAAAGCCTCTAACATTTTGCTCGGTGAAAACTATGAGCCACTGATCTCTGATTTTGGATTAGCGAAGTGGCTGCCTGAAAATTGGGCTCACCATGTCGTCTACCCCATCGAGGGTACGTTTGGATATTTGGCTCCAGAGTACTTCATGCATGGAATCGTTGATGAGAAGACGGATGTTTTCGCTTATGGGGTTTTGTTGCTTGAGCTCATAACTGGGCGCCGTGCAGTTGATTCAAGCCAACAAAGCCTAGTGATTTGGGCAAAGCCTTTACTTGATAACAATAGCTTCCAGGAATTAATGGATCCAAAGTTAGGAGACGACTACGATCAAACTGAAATGAGACGAACCATGTTAGCAGCTTCGATGTGCATAAACCAGTCATCATCGATGCGGCCACATATGAACTCGGTAGTGCAGCTGCTGAAGGGAGAAGAAGGAGCATTAGAACAATTAAAACAGAACGCGATGGATGAGGATCAATCGTGCGCTTCGGAAGCCAGCAACATGGAAGATTACTCATGTAATTCAAACTACCTGGACGACATGAGTCGTCACAGGGAGCTGATTTTGGAGTGA

Protein sequence

FFLLPYFFIFPFVPPAGASASAPSNVTEIPSPFPFILSTLIFFFFFSTGEGIHLFLFFPFLSLIHNYHCPRRLNIDQILKMKVEMEMEFGNARKGIKQMEEMEGYGGLKIIGEEEDLCEENSPRGVFEIPVLGTDSDQSESSSGGDGGSLTVAVNSMLPPPENSLLPSALLQESGGIPWKAMIGLIKKKSVWRLSTIPLLAMSYDISKKNFSKRFARIRSTDEGISIEDVPFCKPSWRIYGFSELATATNNFSPENLLGKGGQAEVYRGCLSDGQFVAVKRLMKREKDNEEKTSDFLTELGIIAHISHPNAARLLGFGIDGGLYLVLEYIPFGSFASALFGAESLDWKIRFKVAIGVADGLNYLHRECPRRIIHRDIKASNILLGENYEPLISDFGLAKWLPENWAHHVVYPIEGTFGYLAPEYFMHGIVDEKTDVFAYGVLLLELITGRRAVDSSQQSLVIWAKPLLDNNSFQELMDPKLGDDYDQTEMRRTMLAASMCINQSSSMRPHMNSVVQLLKGEEGALEQLKQNAMDEDQSCASEASNMEDYSCNSNYLDDMSRHRELILE
Homology
BLAST of Carg14258 vs. NCBI nr
Match: KAG7031325.1 (Receptor-like cytosolic serine/threonine-protein kinase RBK1, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 1137.1 bits (2940), Expect = 0.0e+00
Identity = 568/568 (100.00%), Postives = 568/568 (100.00%), Query Frame = 0

Query: 1   FFLLPYFFIFPFVPPAGASASAPSNVTEIPSPFPFILSTLIFFFFFSTGEGIHLFLFFPF 60
           FFLLPYFFIFPFVPPAGASASAPSNVTEIPSPFPFILSTLIFFFFFSTGEGIHLFLFFPF
Sbjct: 1   FFLLPYFFIFPFVPPAGASASAPSNVTEIPSPFPFILSTLIFFFFFSTGEGIHLFLFFPF 60

Query: 61  LSLIHNYHCPRRLNIDQILKMKVEMEMEFGNARKGIKQMEEMEGYGGLKIIGEEEDLCEE 120
           LSLIHNYHCPRRLNIDQILKMKVEMEMEFGNARKGIKQMEEMEGYGGLKIIGEEEDLCEE
Sbjct: 61  LSLIHNYHCPRRLNIDQILKMKVEMEMEFGNARKGIKQMEEMEGYGGLKIIGEEEDLCEE 120

Query: 121 NSPRGVFEIPVLGTDSDQSESSSGGDGGSLTVAVNSMLPPPENSLLPSALLQESGGIPWK 180
           NSPRGVFEIPVLGTDSDQSESSSGGDGGSLTVAVNSMLPPPENSLLPSALLQESGGIPWK
Sbjct: 121 NSPRGVFEIPVLGTDSDQSESSSGGDGGSLTVAVNSMLPPPENSLLPSALLQESGGIPWK 180

Query: 181 AMIGLIKKKSVWRLSTIPLLAMSYDISKKNFSKRFARIRSTDEGISIEDVPFCKPSWRIY 240
           AMIGLIKKKSVWRLSTIPLLAMSYDISKKNFSKRFARIRSTDEGISIEDVPFCKPSWRIY
Sbjct: 181 AMIGLIKKKSVWRLSTIPLLAMSYDISKKNFSKRFARIRSTDEGISIEDVPFCKPSWRIY 240

Query: 241 GFSELATATNNFSPENLLGKGGQAEVYRGCLSDGQFVAVKRLMKREKDNEEKTSDFLTEL 300
           GFSELATATNNFSPENLLGKGGQAEVYRGCLSDGQFVAVKRLMKREKDNEEKTSDFLTEL
Sbjct: 241 GFSELATATNNFSPENLLGKGGQAEVYRGCLSDGQFVAVKRLMKREKDNEEKTSDFLTEL 300

Query: 301 GIIAHISHPNAARLLGFGIDGGLYLVLEYIPFGSFASALFGAESLDWKIRFKVAIGVADG 360
           GIIAHISHPNAARLLGFGIDGGLYLVLEYIPFGSFASALFGAESLDWKIRFKVAIGVADG
Sbjct: 301 GIIAHISHPNAARLLGFGIDGGLYLVLEYIPFGSFASALFGAESLDWKIRFKVAIGVADG 360

Query: 361 LNYLHRECPRRIIHRDIKASNILLGENYEPLISDFGLAKWLPENWAHHVVYPIEGTFGYL 420
           LNYLHRECPRRIIHRDIKASNILLGENYEPLISDFGLAKWLPENWAHHVVYPIEGTFGYL
Sbjct: 361 LNYLHRECPRRIIHRDIKASNILLGENYEPLISDFGLAKWLPENWAHHVVYPIEGTFGYL 420

Query: 421 APEYFMHGIVDEKTDVFAYGVLLLELITGRRAVDSSQQSLVIWAKPLLDNNSFQELMDPK 480
           APEYFMHGIVDEKTDVFAYGVLLLELITGRRAVDSSQQSLVIWAKPLLDNNSFQELMDPK
Sbjct: 421 APEYFMHGIVDEKTDVFAYGVLLLELITGRRAVDSSQQSLVIWAKPLLDNNSFQELMDPK 480

Query: 481 LGDDYDQTEMRRTMLAASMCINQSSSMRPHMNSVVQLLKGEEGALEQLKQNAMDEDQSCA 540
           LGDDYDQTEMRRTMLAASMCINQSSSMRPHMNSVVQLLKGEEGALEQLKQNAMDEDQSCA
Sbjct: 481 LGDDYDQTEMRRTMLAASMCINQSSSMRPHMNSVVQLLKGEEGALEQLKQNAMDEDQSCA 540

Query: 541 SEASNMEDYSCNSNYLDDMSRHRELILE 569
           SEASNMEDYSCNSNYLDDMSRHRELILE
Sbjct: 541 SEASNMEDYSCNSNYLDDMSRHRELILE 568

BLAST of Carg14258 vs. NCBI nr
Match: KAG6600690.1 (Receptor-like cytosolic serine/threonine-protein kinase RBK1, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 1021.9 bits (2641), Expect = 2.1e-294
Identity = 517/538 (96.10%), Postives = 520/538 (96.65%), Query Frame = 0

Query: 32  PFPFILSTLIFFFFFST-GEGIHLFLFFPFLSLIHNYHCPRRLNIDQILKMKVEMEMEFG 91
           P   I +T++     ST GEGIHLF FF       NYHCPRRLNIDQILKMKVEMEMEFG
Sbjct: 48  PQRHIAATVLTLLGRSTEGEGIHLFFFF-------NYHCPRRLNIDQILKMKVEMEMEFG 107

Query: 92  NARKGIKQMEEMEGYGGLKIIGEEEDLCEENSPRGVFEIPVLGTDSDQSESSSGGDGGSL 151
           NARKGIKQMEEMEGYGGLKIIGEEEDLCEENSPRGVFEIPVLGTDSDQSESSSGGDGGSL
Sbjct: 108 NARKGIKQMEEMEGYGGLKIIGEEEDLCEENSPRGVFEIPVLGTDSDQSESSSGGDGGSL 167

Query: 152 TVAVNSMLPPPENSLLPSALLQESGGIPWKAMIGLIKKKSVWRLSTIPLLAMSYDISKKN 211
           TVAVNSMLPPPENSLLPSALLQESGGIPWKAMIGLIKKKSVWRLSTIPLLAMSYDISKKN
Sbjct: 168 TVAVNSMLPPPENSLLPSALLQESGGIPWKAMIGLIKKKSVWRLSTIPLLAMSYDISKKN 227

Query: 212 FSKRFARIRSTDEGISIEDVPFCKPSWRIYGFSELATATNNFSPENLLGKGGQAEVYRGC 271
           FSKRFARIRSTDEGISIEDVPFCKPSWRIYGFSELATATNNFSPENLLGKGGQAEVYRGC
Sbjct: 228 FSKRFARIRSTDEGISIEDVPFCKPSWRIYGFSELATATNNFSPENLLGKGGQAEVYRGC 287

Query: 272 LSDGQFVAVKRLMKREKDNEEKTSDFLTELGIIAHISHPNAARLLGFGIDGGLYLVLEYI 331
           LSDGQFVAVKRLMKREKDNEEKTSDFLTELGIIAHISHPNAARLLGFGIDGGLYLVLEYI
Sbjct: 288 LSDGQFVAVKRLMKREKDNEEKTSDFLTELGIIAHISHPNAARLLGFGIDGGLYLVLEYI 347

Query: 332 PFGSFASALFGAESLDWKIRFKVAIGVADGLNYLHRECPRRIIHRDIKASNILLGENYEP 391
           PFGSFASALFGAESLDWKIRFKVAIGVADGLNYLHRECPRRIIHRDIKASNILLGENYEP
Sbjct: 348 PFGSFASALFGAESLDWKIRFKVAIGVADGLNYLHRECPRRIIHRDIKASNILLGENYEP 407

Query: 392 LISDFGLAKWLPENWAHHVVYPIEGTFGYLAPEYFMHGIVDEKTDVFAYGVLLLELITGR 451
           LISDFGLAKWLPENWAHHVVYPIEGTFGYLAPEYFMHGIVDEKTDVFAYGVLLLELITGR
Sbjct: 408 LISDFGLAKWLPENWAHHVVYPIEGTFGYLAPEYFMHGIVDEKTDVFAYGVLLLELITGR 467

Query: 452 RAVDSSQQSLVIWAKPLLDNNSFQELMDPKLGDDYDQTEMRRTMLAASMCINQSSSMRPH 511
           RAVDSSQQSLVIWAKPLLDNNSFQELMDPKLGDDYDQTEMRRTMLAASMCINQSSSMRPH
Sbjct: 468 RAVDSSQQSLVIWAKPLLDNNSFQELMDPKLGDDYDQTEMRRTMLAASMCINQSSSMRPH 527

Query: 512 MNSVVQLLKGEEGALEQLKQNAMDEDQSCASEASNMEDYSCNSNYLDDMSRHRELILE 569
           MNSVVQLLKGEEGALEQLKQNAMDEDQSCASEASNMEDYSCNSNYLDDMSRHRELILE
Sbjct: 528 MNSVVQLLKGEEGALEQLKQNAMDEDQSCASEASNMEDYSCNSNYLDDMSRHRELILE 578

BLAST of Carg14258 vs. NCBI nr
Match: XP_023548346.1 (receptor-like cytosolic serine/threonine-protein kinase RBK1 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 976.1 bits (2522), Expect = 1.3e-280
Identity = 485/488 (99.39%), Postives = 486/488 (99.59%), Query Frame = 0

Query: 81  MKVEMEMEFGNARKGIKQMEEMEGYGGLKIIGEEEDLCEENSPRGVFEIPVLGTDSDQSE 140
           MKVEMEMEFGNARKGIK MEEMEGYGGLKIIGEEEDLCEENSPRGVFEIPVLGTDSDQSE
Sbjct: 1   MKVEMEMEFGNARKGIKHMEEMEGYGGLKIIGEEEDLCEENSPRGVFEIPVLGTDSDQSE 60

Query: 141 SSSGGDGGSLTVAVNSMLPPPENSLLPSALLQESGGIPWKAMIGLIKKKSVWRLSTIPLL 200
           SSSGGDGGSLTVAVNSMLPPPENSLLPSALLQESGGIPWKAMIGLIKKKSVWRLSTIPLL
Sbjct: 61  SSSGGDGGSLTVAVNSMLPPPENSLLPSALLQESGGIPWKAMIGLIKKKSVWRLSTIPLL 120

Query: 201 AMSYDISKKNFSKRFARIRSTDEGISIEDVPFCKPSWRIYGFSELATATNNFSPENLLGK 260
           AMSYDISKKNFSKRFARIRSTDEGISIEDVPFCKPSWRIYGFSEL+TATNNFSPENLLGK
Sbjct: 121 AMSYDISKKNFSKRFARIRSTDEGISIEDVPFCKPSWRIYGFSELSTATNNFSPENLLGK 180

Query: 261 GGQAEVYRGCLSDGQFVAVKRLMKREKDNEEKTSDFLTELGIIAHISHPNAARLLGFGID 320
           GGQAEVYRGCLSDGQFVAVKRLMKREKDNEEKTSDFLTELGIIAHISHPNAARLLGFGID
Sbjct: 181 GGQAEVYRGCLSDGQFVAVKRLMKREKDNEEKTSDFLTELGIIAHISHPNAARLLGFGID 240

Query: 321 GGLYLVLEYIPFGSFASALFGAESLDWKIRFKVAIGVADGLNYLHRECPRRIIHRDIKAS 380
           GGLYLVLEYIPFGSFASALFGAESLDWKIRFKVAIGVADGLNYLHRECPRRIIHRDIKAS
Sbjct: 241 GGLYLVLEYIPFGSFASALFGAESLDWKIRFKVAIGVADGLNYLHRECPRRIIHRDIKAS 300

Query: 381 NILLGENYEPLISDFGLAKWLPENWAHHVVYPIEGTFGYLAPEYFMHGIVDEKTDVFAYG 440
           NILLGENYEPLISDFGLAKWLPENWAHHVVYPIEGTFGYLAPEYFMHGIVDEKTDVFAYG
Sbjct: 301 NILLGENYEPLISDFGLAKWLPENWAHHVVYPIEGTFGYLAPEYFMHGIVDEKTDVFAYG 360

Query: 441 VLLLELITGRRAVDSSQQSLVIWAKPLLDNNSFQELMDPKLGDDYDQTEMRRTMLAASMC 500
           VLLLELITGRRAVDSSQQSLVIWAKPLLDNNSFQELMDPKLGDDYDQTEMRRTMLAASMC
Sbjct: 361 VLLLELITGRRAVDSSQQSLVIWAKPLLDNNSFQELMDPKLGDDYDQTEMRRTMLAASMC 420

Query: 501 INQSSSMRPHMNSVVQLLKGEEGALEQLKQNAMDEDQSCASEASNMEDYSCNSNYLDDMS 560
           INQSSSMRPHMNSVVQLLKGEEGALEQLKQNAMDEDQSC SEASNMEDYSCNSNYLDDMS
Sbjct: 421 INQSSSMRPHMNSVVQLLKGEEGALEQLKQNAMDEDQSCTSEASNMEDYSCNSNYLDDMS 480

Query: 561 RHRELILE 569
           RHRELILE
Sbjct: 481 RHRELILE 488

BLAST of Carg14258 vs. NCBI nr
Match: XP_022941642.1 (receptor-like cytosolic serine/threonine-protein kinase RBK1 [Cucurbita moschata])

HSP 1 Score: 974.9 bits (2519), Expect = 2.9e-280
Identity = 484/488 (99.18%), Postives = 486/488 (99.59%), Query Frame = 0

Query: 81  MKVEMEMEFGNARKGIKQMEEMEGYGGLKIIGEEEDLCEENSPRGVFEIPVLGTDSDQSE 140
           MKVEMEMEFGNARKGIKQMEEMEGYGGLKIIGEEEDLCEENSPRGVFEIPVLGTDSDQSE
Sbjct: 1   MKVEMEMEFGNARKGIKQMEEMEGYGGLKIIGEEEDLCEENSPRGVFEIPVLGTDSDQSE 60

Query: 141 SSSGGDGGSLTVAVNSMLPPPENSLLPSALLQESGGIPWKAMIGLIKKKSVWRLSTIPLL 200
           SSSGGDGGSLTVAVNSMLPPPENSLLPSALLQESGGIPWKAMIGLIKKKSVWRLSTIPLL
Sbjct: 61  SSSGGDGGSLTVAVNSMLPPPENSLLPSALLQESGGIPWKAMIGLIKKKSVWRLSTIPLL 120

Query: 201 AMSYDISKKNFSKRFARIRSTDEGISIEDVPFCKPSWRIYGFSELATATNNFSPENLLGK 260
           AMSYDISKKNFSKRFARIRSTDEGISIEDVPFCKPSWRIYGFSELATATNNFSPENLLGK
Sbjct: 121 AMSYDISKKNFSKRFARIRSTDEGISIEDVPFCKPSWRIYGFSELATATNNFSPENLLGK 180

Query: 261 GGQAEVYRGCLSDGQFVAVKRLMKREKDNEEKTSDFLTELGIIAHISHPNAARLLGFGID 320
           GGQAEVYRGCLSDGQFVAVKRLMKREKDNEEKTSDFLTELGIIAHISHPNAARLLGFGID
Sbjct: 181 GGQAEVYRGCLSDGQFVAVKRLMKREKDNEEKTSDFLTELGIIAHISHPNAARLLGFGID 240

Query: 321 GGLYLVLEYIPFGSFASALFGAESLDWKIRFKVAIGVADGLNYLHRECPRRIIHRDIKAS 380
           GGLYLVLEYIPFGSFASALFGAESLDWKIRFKVAIGVADGLNYLHRECPRRIIHRDIKAS
Sbjct: 241 GGLYLVLEYIPFGSFASALFGAESLDWKIRFKVAIGVADGLNYLHRECPRRIIHRDIKAS 300

Query: 381 NILLGENYEPLISDFGLAKWLPENWAHHVVYPIEGTFGYLAPEYFMHGIVDEKTDVFAYG 440
           NILLGENYEPLISDFGLAKWLPENWAHHVVYPIEGTFGYLAPEYFMHGIVDEKTDVFAYG
Sbjct: 301 NILLGENYEPLISDFGLAKWLPENWAHHVVYPIEGTFGYLAPEYFMHGIVDEKTDVFAYG 360

Query: 441 VLLLELITGRRAVDSSQQSLVIWAKPLLDNNSFQELMDPKLGDDYDQTEMRRTMLAASMC 500
           VLLLELITGRRAVDSSQQSLVIWAKPLLDNNSFQELMDPKLGDDYDQTEMRRTMLAASMC
Sbjct: 361 VLLLELITGRRAVDSSQQSLVIWAKPLLDNNSFQELMDPKLGDDYDQTEMRRTMLAASMC 420

Query: 501 INQSSSMRPHMNSVVQLLKGEEGALEQLKQNAMDEDQSCASEASNMEDYSCNSNYLDDMS 560
           IN SSSMRPHMNSVVQLLKGEEGALEQLKQNAMD+DQ+C SEASNMEDYSCNSNYLDDMS
Sbjct: 421 INHSSSMRPHMNSVVQLLKGEEGALEQLKQNAMDQDQTCVSEASNMEDYSCNSNYLDDMS 480

Query: 561 RHRELILE 569
           RHRELILE
Sbjct: 481 RHRELILE 488

BLAST of Carg14258 vs. NCBI nr
Match: XP_022992501.1 (receptor-like cytosolic serine/threonine-protein kinase RBK1 [Cucurbita maxima] >XP_022992512.1 receptor-like cytosolic serine/threonine-protein kinase RBK1 [Cucurbita maxima] >XP_022992521.1 receptor-like cytosolic serine/threonine-protein kinase RBK1 [Cucurbita maxima])

HSP 1 Score: 957.6 bits (2474), Expect = 4.8e-275
Identity = 478/488 (97.95%), Postives = 480/488 (98.36%), Query Frame = 0

Query: 81  MKVEMEMEFGNARKGIKQMEEMEGYGGLKIIGEEEDLCEENSPRGVFEIPVLGTDSDQSE 140
           MKVEMEMEFGNARKGIKQMEEMEGYGGLKIIGEEEDLCEENSPRGVFEIPV+GTDSDQS 
Sbjct: 1   MKVEMEMEFGNARKGIKQMEEMEGYGGLKIIGEEEDLCEENSPRGVFEIPVVGTDSDQSV 60

Query: 141 SSSGGDGGSLTVAVNSMLPPPENSLLPSALLQESGGIPWKAMIGLIKKKSVWRLSTIPLL 200
           SSSGGDGGSLTVAVNSMLPP ENSLLPS LLQESGGIPWKAMIGLIKKKS WRLSTIPLL
Sbjct: 61  SSSGGDGGSLTVAVNSMLPPAENSLLPSVLLQESGGIPWKAMIGLIKKKSAWRLSTIPLL 120

Query: 201 AMSYDISKKNFSKRFARIRSTDEGISIEDVPFCKPSWRIYGFSELATATNNFSPENLLGK 260
           AMSYDISKKNFSKRFARIRSTDEGISIEDVPFCKPSWRIYGFSELATATNNFSPENLLGK
Sbjct: 121 AMSYDISKKNFSKRFARIRSTDEGISIEDVPFCKPSWRIYGFSELATATNNFSPENLLGK 180

Query: 261 GGQAEVYRGCLSDGQFVAVKRLMKREKDNEEKTSDFLTELGIIAHISHPNAARLLGFGID 320
           GGQAEVYRGCLSDGQFVAVKRLMKREKDNEEK SDFLTELGIIAHISHPNAARLLGFGID
Sbjct: 181 GGQAEVYRGCLSDGQFVAVKRLMKREKDNEEKASDFLTELGIIAHISHPNAARLLGFGID 240

Query: 321 GGLYLVLEYIPFGSFASALFGAESLDWKIRFKVAIGVADGLNYLHRECPRRIIHRDIKAS 380
           GGLYLVLEYIPFGSFASALFGAESLDWKIRFKVAIGVADGLNYLHRECPRRIIHRDIKAS
Sbjct: 241 GGLYLVLEYIPFGSFASALFGAESLDWKIRFKVAIGVADGLNYLHRECPRRIIHRDIKAS 300

Query: 381 NILLGENYEPLISDFGLAKWLPENWAHHVVYPIEGTFGYLAPEYFMHGIVDEKTDVFAYG 440
           NILLGENYEPLISDFGLAKWLPENWAHHVVYPIEGTFGYLAPEYFMHGIVDEKTDVFAYG
Sbjct: 301 NILLGENYEPLISDFGLAKWLPENWAHHVVYPIEGTFGYLAPEYFMHGIVDEKTDVFAYG 360

Query: 441 VLLLELITGRRAVDSSQQSLVIWAKPLLDNNSFQELMDPKLGDDYDQTEMRRTMLAASMC 500
           VLLLELITGRRAVDSSQQSLVIWAKPLLDNNSFQELMDPKLGDDYDQTEMRRTMLAASMC
Sbjct: 361 VLLLELITGRRAVDSSQQSLVIWAKPLLDNNSFQELMDPKLGDDYDQTEMRRTMLAASMC 420

Query: 501 INQSSSMRPHMNSVVQLLKGEEGALEQLKQNAMDEDQSCASEASNMEDYSCNSNYLDDMS 560
           IN SSSMRPHMNSVVQLLKG EGALEQLKQNAMDEDQSC SEA+NMEDYSCNSNYLDDMS
Sbjct: 421 INHSSSMRPHMNSVVQLLKG-EGALEQLKQNAMDEDQSCVSEANNMEDYSCNSNYLDDMS 480

Query: 561 RHRELILE 569
           RHRELILE
Sbjct: 481 RHRELILE 487

BLAST of Carg14258 vs. ExPASy Swiss-Prot
Match: Q8H1D6 (Receptor-like cytosolic serine/threonine-protein kinase RBK1 OS=Arabidopsis thaliana OX=3702 GN=RBK1 PE=1 SV=1)

HSP 1 Score: 483.0 bits (1242), Expect = 4.6e-135
Identity = 249/452 (55.09%), Postives = 330/452 (73.01%), Query Frame = 0

Query: 119 EENSPRGVFEIPVLGTDSDQSESSSGGDGGSLTVAVNSMLPPPENSLLPSALLQESGGIP 178
           + +SPRG     VLG  SD   SSS     S     +S   P  N+     +     G+ 
Sbjct: 28  DSSSPRG-----VLGMVSDSDNSSSSCSSCSSDDKSSSTSSPFSNT--TKTVSSSHHGLQ 87

Query: 179 WKAMIGLIKKKSVWRLSTIPLLAMSYDISKKNFSKRFARIRSTDEGISIEDVPFCKPSWR 238
           W  MI  IKKKS+ R S IPLLA SY++++KN  ++  ++  ++   + E     KPSWR
Sbjct: 88  WNKMIESIKKKSMRRFSVIPLLA-SYELTRKNLRRKQPKLTPSESAFTCEAFFMAKPSWR 147

Query: 239 IYGFSELATATNNFSPENLLGKGGQAEVYRGCLSDGQFVAVKRLMKREKDNEEKTSDFLT 298
            + + ELA AT+ F+PEN++GKGG AEVY+G L +G+ VA+K+LM   K+ EE+ SDFL+
Sbjct: 148 NFTYEELAVATDYFNPENMIGKGGHAEVYKGVLINGETVAIKKLMSHAKEEEERVSDFLS 207

Query: 299 ELGIIAHISHPNAARLLGFGIDGGLYLVLEYIPFGSFASALFGAES-LDWKIRFKVAIGV 358
           ELGIIAH++HPNAARL GF  D GL+ VLEY P+GS AS LFG+E  L+WKIR+KVA+G+
Sbjct: 208 ELGIIAHVNHPNAARLRGFSSDRGLHFVLEYAPYGSLASMLFGSEECLEWKIRYKVALGI 267

Query: 359 ADGLNYLHRECPRRIIHRDIKASNILLGENYEPLISDFGLAKWLPENWAHHVVYPIEGTF 418
           ADGL+YLH  CPRRIIHRDIKASNILL  +YE  ISDFGLAKWLPENW HHVV+PIEGTF
Sbjct: 268 ADGLSYLHNACPRRIIHRDIKASNILLNHDYEAQISDFGLAKWLPENWPHHVVFPIEGTF 327

Query: 419 GYLAPEYFMHGIVDEKTDVFAYGVLLLELITGRRAVD-SSQQSLVIWAKPLLDNNSFQEL 478
           GYLAPEYFMHGIVDEK DVFA+GVLLLE+IT RRAVD +S+QS+V WAKP L+ NS +++
Sbjct: 328 GYLAPEYFMHGIVDEKIDVFAFGVLLLEIITSRRAVDTASRQSIVAWAKPFLEKNSMEDI 387

Query: 479 MDPKLGDDYDQTEMRRTMLAASMCINQSSSMRPHMNSVVQLLKGEEGALEQLKQNAMDED 538
           +DP+LG+ ++ TEM+R ML ASMC++  ++MRP M  +VQLL+GE+G  E L+Q A +  
Sbjct: 388 VDPRLGNMFNPTEMQRVMLTASMCVHHIAAMRPDMTRLVQLLRGEDGPAE-LQQKAGERT 447

Query: 539 QSCASEASNMEDYSCNSNYLDDMSRHRELILE 569
            S    A +++D++ +S+YL+++ RHR+L++E
Sbjct: 448 MSV--NACDLQDHT-SSSYLNELRRHRQLLME 467

BLAST of Carg14258 vs. ExPASy Swiss-Prot
Match: Q8RXC8 (Receptor-like cytosolic serine/threonine-protein kinase RBK2 OS=Arabidopsis thaliana OX=3702 GN=RBK2 PE=1 SV=1)

HSP 1 Score: 368.2 bits (944), Expect = 1.6e-100
Identity = 215/474 (45.36%), Postives = 288/474 (60.76%), Query Frame = 0

Query: 108 LKIIGEEEDLCEENSPRGVFEIPVLGTDSDQSESSSGGDGGSLTVAVNSMLPPPENSLLP 167
           L+++  +++  +  SPRG  E   L   S  S SSS  D            PPP    + 
Sbjct: 9   LRLLEVDKEKQDPKSPRGALE-ACLTRCSISSASSSSDD------------PPPNREAID 68

Query: 168 SA-------LLQESGGIPWKAMIGLIKKKSVWRLSTI-PLLAMSYDISKKNFSKRFARIR 227
           +A                W     L K++S+ RLS+  PL   +  I K+N        +
Sbjct: 69  NADADTDVQCKNHRASSNWGKFFKLWKRRSMKRLSSFPPLSGAAPPIIKQN--------K 128

Query: 228 STD---EGISIEDVPFCKPSWRIYGFSELATATNNFSPENLLGKGGQAEVYRGCLSDGQF 287
           S D    G+ + D+   + S + +  S++  AT+NFSPEN++G+GG A+VY+G L +G+ 
Sbjct: 129 SADPNMNGMVLHDIYDFQSSLQNFSISDIEIATDNFSPENIIGRGGYADVYQGILPEGKL 188

Query: 288 VAVKRLMKREKDNEEKTSDFLTELGIIAHISHPNAARLLGFGIDGGLYLVLEYIPFGSFA 347
           +AVKRL K   D  E+T++FL+ELGIIAH+ HPN A+ +G  I+GG++LV    P GS  
Sbjct: 189 IAVKRLTKGTPD--EQTAEFLSELGIIAHVDHPNTAKFIGCCIEGGMHLVFRLSPLGSLG 248

Query: 348 SALFGAE--SLDWKIRFKVAIGVADGLNYLHRECPRRIIHRDIKASNILLGENYEPLISD 407
           S L G     L W  R+ VA+G ADGL YLH  C RRIIHRDIKA NILL E+++P I D
Sbjct: 249 SLLHGPSKYKLTWSRRYNVALGTADGLVYLHEGCQRRIIHRDIKADNILLTEDFQPQICD 308

Query: 408 FGLAKWLPENWAHHVVYPIEGTFGYLAPEYFMHGIVDEKTDVFAYGVLLLELITGRRAVD 467
           FGLAKWLP+   HH V   EGTFGY APEYFMHGIVDEKTDVFA+GVLLLELITG  A+D
Sbjct: 309 FGLAKWLPKQLTHHNVSKFEGTFGYFAPEYFMHGIVDEKTDVFAFGVLLLELITGHPALD 368

Query: 468 SSQQSLVIWAKPLLDNNSFQELMDPKLGDDYDQTEMRRTMLAASMCINQSSSMRPHMNSV 527
            SQQSLV+WAKPLL+  + +EL+DP LGD+Y++ E+ R    AS+CI+QSS +RP M+ V
Sbjct: 369 ESQQSLVLWAKPLLERKAIKELVDPSLGDEYNREELIRLTSTASLCIDQSSLLRPRMSQV 428

Query: 528 VQLLKGEEGALEQLKQNAMDEDQSCASEASNMEDYSCNS-NYLDDMSRHRELIL 568
           V+LL G E  +   ++  +   Q   SE   ++    NS  YL D+ R RE+ L
Sbjct: 429 VELLLGHEDVVMTPREAKIKMMQRTYSE-ELLDSVEYNSTKYLGDLDRIREVAL 458

BLAST of Carg14258 vs. ExPASy Swiss-Prot
Match: Q5XF57 (Probable receptor-like serine/threonine-protein kinase At5g57670 OS=Arabidopsis thaliana OX=3702 GN=At5g57670 PE=2 SV=1)

HSP 1 Score: 333.6 bits (854), Expect = 4.5e-90
Identity = 170/336 (50.60%), Postives = 232/336 (69.05%), Query Frame = 0

Query: 224 GISIEDVPFCKPSWRIYGFSELATATNNFSPENLLGKGGQAEVYRGCLSDGQFVAVKRLM 283
           G   ++ P  +P  + + ++E++ ATN+F   N++G GG +EVYRG L DG+ +AVKRL 
Sbjct: 239 GSETKNKPKPQPLIQCFTYNEISKATNDFHQGNIVGIGGYSEVYRGDLWDGRRIAVKRLA 298

Query: 284 KREKD-NEEKTSDFLTELGIIAHISHPNAARLLGFGIDGGLYLVLEYIPFGSFASALFGA 343
           K   D N+EK  +FLTELGII+H+SHPN A LLG  ++ GLYLV  +   G+  SAL   
Sbjct: 299 KESGDMNKEK--EFLTELGIISHVSHPNTALLLGCCVEKGLYLVFRFSENGTLYSALHEN 358

Query: 344 E--SLDWKIRFKVAIGVADGLNYLHRECPRRIIHRDIKASNILLGENYEPLISDFGLAKW 403
           E  SLDW +R+K+A+GVA GL+YLH+ C  RIIHRDIK+SN+LLG +YEP I+DFGLAKW
Sbjct: 359 ENGSLDWPVRYKIAVGVARGLHYLHKRCNHRIIHRDIKSSNVLLGPDYEPQITDFGLAKW 418

Query: 404 LPENWAHHVVYPIEGTFGYLAPEYFMHGIVDEKTDVFAYGVLLLELITGRRAVDSSQQSL 463
           LP  W HH V P+EGTFGYLAPE  M G +DEKTD++A+G+LLLE+ITGRR V+ +Q+ +
Sbjct: 419 LPNKWTHHAVIPVEGTFGYLAPESLMQGTIDEKTDIYAFGILLLEIITGRRPVNPTQKHI 478

Query: 464 VIWAKPLLDNNSFQELMDPKLGDDYDQTEMRRTMLAASMCINQSSSMRPHMNSVVQLLKG 523
           ++WAKP ++  +  EL+DPKL D YD  +M + +L AS C+ QS  +RP M  V++LL  
Sbjct: 479 LLWAKPAMETGNTSELVDPKLQDKYDDQQMNKLVLTASHCVQQSPILRPTMTQVLELLTN 538

Query: 524 EEGALEQLKQNAMDEDQSCASEASN-MEDYSCNSNY 556
              A E  K   M +D +   + +N  +DYS    Y
Sbjct: 539 GNEA-EIAKSWRMPKDMTNDDDDNNEWDDYSMIFGY 571

BLAST of Carg14258 vs. ExPASy Swiss-Prot
Match: Q8RWW0 (Receptor-like serine/threonine-protein kinase ALE2 OS=Arabidopsis thaliana OX=3702 GN=ALE2 PE=1 SV=1)

HSP 1 Score: 240.4 bits (612), Expect = 5.1e-62
Identity = 143/363 (39.39%), Postives = 212/363 (58.40%), Query Frame = 0

Query: 169 ALLQESGGIPWKAMIG----LIKKKSVWRLSTI--PLLAMSYDISKKNFSKRFARIRSTD 228
           A++  SG +    ++G    ++K K + + S    P LA S +      S   +  RS+ 
Sbjct: 260 AIIALSGFVLILVLVGAISIIVKWKKIGKSSNAVGPALAPSINKRPGAGSMFSSSARSSG 319

Query: 229 EGISIEDVPFCKPSWRIYGFSELATATNNFSPENLLGKGGQAEVYRGCLSDGQFVAVKRL 288
               +  +  C  S + +  SEL  AT+ FS + +LG+GG   VY+G + DG  VAVK L
Sbjct: 320 SDSLMSSMATCALSVKTFTLSELEKATDRFSAKRVLGEGGFGRVYQGSMEDGTEVAVKLL 379

Query: 289 MKREKDNEEKTSDFLTELGIIAHISHPNAARLLGFGIDGGL-YLVLEYIPFGSFASALFG 348
               +DN+ +  +F+ E+ +++ + H N  +L+G  I+G    L+ E +  GS  S L  
Sbjct: 380 ---TRDNQNRDREFIAEVEMLSRLHHRNLVKLIGICIEGRTRCLIYELVHNGSVESHLHE 439

Query: 349 AESLDWKIRFKVAIGVADGLNYLHRECPRRIIHRDIKASNILLGENYEPLISDFGLAKWL 408
             +LDW  R K+A+G A GL YLH +   R+IHRD KASN+LL +++ P +SDFGLA+  
Sbjct: 440 G-TLDWDARLKIALGAARGLAYLHEDSNPRVIHRDFKASNVLLEDDFTPKVSDFGLAREA 499

Query: 409 PENWAHHVVYPIEGTFGYLAPEYFMHGIVDEKTDVFAYGVLLLELITGRRAVDSSQ---- 468
            E  + H+   + GTFGY+APEY M G +  K+DV++YGV+LLEL+TGRR VD SQ    
Sbjct: 500 TEG-SQHISTRVMGTFGYVAPEYAMTGHLLVKSDVYSYGVVLLELLTGRRPVDMSQPSGE 559

Query: 469 QSLVIWAKPLLDN-NSFQELMDPKLGDDYDQTEMRRTMLAASMCINQSSSMRPHMNSVVQ 520
           ++LV WA+PLL N    ++L+DP L   Y+  +M +    ASMC++Q  S RP M  VVQ
Sbjct: 560 ENLVTWARPLLANREGLEQLVDPALAGTYNFDDMAKVAAIASMCVHQEVSHRPFMGEVVQ 617

BLAST of Carg14258 vs. ExPASy Swiss-Prot
Match: Q9LV48 (Proline-rich receptor-like protein kinase PERK1 OS=Arabidopsis thaliana OX=3702 GN=PERK1 PE=1 SV=1)

HSP 1 Score: 237.7 bits (605), Expect = 3.3e-61
Identity = 145/368 (39.40%), Postives = 209/368 (56.79%), Query Frame = 0

Query: 220 STDEGISIEDVPFCKP---------SWRIYGFSELATATNNFSPENLLGKGGQAEVYRGC 279
           S+  G    D+P   P         S   + + EL+ ATN FS  NLLG+GG   V++G 
Sbjct: 239 SSSGGSDYSDLPVLPPPSPGLVLGFSKSTFTYEELSRATNGFSEANLLGQGGFGYVHKGI 298

Query: 280 LSDGQFVAVKRLMKREKDNEEKTSDFLTELGIIAHISHPNAARLLGFGIDG-GLYLVLEY 339
           L  G+ VAVK+L       E    +F  E+ II+ + H +   L+G+ + G    LV E+
Sbjct: 299 LPSGKEVAVKQLKAGSGQGER---EFQAEVEIISRVHHRHLVSLIGYCMAGVQRLLVYEF 358

Query: 340 IPFGSFASALF--GAESLDWKIRFKVAIGVADGLNYLHRECPRRIIHRDIKASNILLGEN 399
           +P  +    L   G  +++W  R K+A+G A GL+YLH +C  +IIHRDIKASNIL+   
Sbjct: 359 VPNNNLEFHLHGKGRPTMEWSTRLKIALGSAKGLSYLHEDCNPKIIHRDIKASNILIDFK 418

Query: 400 YEPLISDFGLAKWLPENWAHHVVYPIEGTFGYLAPEYFMHGIVDEKTDVFAYGVLLLELI 459
           +E  ++DFGLAK +  +   HV   + GTFGYLAPEY   G + EK+DVF++GV+LLELI
Sbjct: 419 FEAKVADFGLAK-IASDTNTHVSTRVMGTFGYLAPEYAASGKLTEKSDVFSFGVVLLELI 478

Query: 460 TGRRAVDSS----QQSLVIWAKPLLDNNS----FQELMDPKLGDDYDQTEMRRTMLAASM 519
           TGRR VD++      SLV WA+PLL+  S    F+ L D K+G++YD+ EM R +  A+ 
Sbjct: 479 TGRRPVDANNVYVDDSLVDWARPLLNRASEEGDFEGLADSKMGNEYDREEMARMVACAAA 538

Query: 520 CINQSSSMRPHMNSVVQLLKGEEGALEQLKQNAMDEDQSCASEASNMEDYSCNSNYLDDM 568
           C+  S+  RP M+ +V+ L+G   +L  L +       +  S      DY   S Y DDM
Sbjct: 539 CVRHSARRRPRMSQIVRALEGNV-SLSDLNEGMRPGHSNVYSSYGGSTDYD-TSQYNDDM 598

BLAST of Carg14258 vs. ExPASy TrEMBL
Match: A0A6J1FN21 (receptor-like cytosolic serine/threonine-protein kinase RBK1 OS=Cucurbita moschata OX=3662 GN=LOC111446941 PE=4 SV=1)

HSP 1 Score: 974.9 bits (2519), Expect = 1.4e-280
Identity = 484/488 (99.18%), Postives = 486/488 (99.59%), Query Frame = 0

Query: 81  MKVEMEMEFGNARKGIKQMEEMEGYGGLKIIGEEEDLCEENSPRGVFEIPVLGTDSDQSE 140
           MKVEMEMEFGNARKGIKQMEEMEGYGGLKIIGEEEDLCEENSPRGVFEIPVLGTDSDQSE
Sbjct: 1   MKVEMEMEFGNARKGIKQMEEMEGYGGLKIIGEEEDLCEENSPRGVFEIPVLGTDSDQSE 60

Query: 141 SSSGGDGGSLTVAVNSMLPPPENSLLPSALLQESGGIPWKAMIGLIKKKSVWRLSTIPLL 200
           SSSGGDGGSLTVAVNSMLPPPENSLLPSALLQESGGIPWKAMIGLIKKKSVWRLSTIPLL
Sbjct: 61  SSSGGDGGSLTVAVNSMLPPPENSLLPSALLQESGGIPWKAMIGLIKKKSVWRLSTIPLL 120

Query: 201 AMSYDISKKNFSKRFARIRSTDEGISIEDVPFCKPSWRIYGFSELATATNNFSPENLLGK 260
           AMSYDISKKNFSKRFARIRSTDEGISIEDVPFCKPSWRIYGFSELATATNNFSPENLLGK
Sbjct: 121 AMSYDISKKNFSKRFARIRSTDEGISIEDVPFCKPSWRIYGFSELATATNNFSPENLLGK 180

Query: 261 GGQAEVYRGCLSDGQFVAVKRLMKREKDNEEKTSDFLTELGIIAHISHPNAARLLGFGID 320
           GGQAEVYRGCLSDGQFVAVKRLMKREKDNEEKTSDFLTELGIIAHISHPNAARLLGFGID
Sbjct: 181 GGQAEVYRGCLSDGQFVAVKRLMKREKDNEEKTSDFLTELGIIAHISHPNAARLLGFGID 240

Query: 321 GGLYLVLEYIPFGSFASALFGAESLDWKIRFKVAIGVADGLNYLHRECPRRIIHRDIKAS 380
           GGLYLVLEYIPFGSFASALFGAESLDWKIRFKVAIGVADGLNYLHRECPRRIIHRDIKAS
Sbjct: 241 GGLYLVLEYIPFGSFASALFGAESLDWKIRFKVAIGVADGLNYLHRECPRRIIHRDIKAS 300

Query: 381 NILLGENYEPLISDFGLAKWLPENWAHHVVYPIEGTFGYLAPEYFMHGIVDEKTDVFAYG 440
           NILLGENYEPLISDFGLAKWLPENWAHHVVYPIEGTFGYLAPEYFMHGIVDEKTDVFAYG
Sbjct: 301 NILLGENYEPLISDFGLAKWLPENWAHHVVYPIEGTFGYLAPEYFMHGIVDEKTDVFAYG 360

Query: 441 VLLLELITGRRAVDSSQQSLVIWAKPLLDNNSFQELMDPKLGDDYDQTEMRRTMLAASMC 500
           VLLLELITGRRAVDSSQQSLVIWAKPLLDNNSFQELMDPKLGDDYDQTEMRRTMLAASMC
Sbjct: 361 VLLLELITGRRAVDSSQQSLVIWAKPLLDNNSFQELMDPKLGDDYDQTEMRRTMLAASMC 420

Query: 501 INQSSSMRPHMNSVVQLLKGEEGALEQLKQNAMDEDQSCASEASNMEDYSCNSNYLDDMS 560
           IN SSSMRPHMNSVVQLLKGEEGALEQLKQNAMD+DQ+C SEASNMEDYSCNSNYLDDMS
Sbjct: 421 INHSSSMRPHMNSVVQLLKGEEGALEQLKQNAMDQDQTCVSEASNMEDYSCNSNYLDDMS 480

Query: 561 RHRELILE 569
           RHRELILE
Sbjct: 481 RHRELILE 488

BLAST of Carg14258 vs. ExPASy TrEMBL
Match: A0A6J1JTS6 (receptor-like cytosolic serine/threonine-protein kinase RBK1 OS=Cucurbita maxima OX=3661 GN=LOC111488816 PE=4 SV=1)

HSP 1 Score: 957.6 bits (2474), Expect = 2.3e-275
Identity = 478/488 (97.95%), Postives = 480/488 (98.36%), Query Frame = 0

Query: 81  MKVEMEMEFGNARKGIKQMEEMEGYGGLKIIGEEEDLCEENSPRGVFEIPVLGTDSDQSE 140
           MKVEMEMEFGNARKGIKQMEEMEGYGGLKIIGEEEDLCEENSPRGVFEIPV+GTDSDQS 
Sbjct: 1   MKVEMEMEFGNARKGIKQMEEMEGYGGLKIIGEEEDLCEENSPRGVFEIPVVGTDSDQSV 60

Query: 141 SSSGGDGGSLTVAVNSMLPPPENSLLPSALLQESGGIPWKAMIGLIKKKSVWRLSTIPLL 200
           SSSGGDGGSLTVAVNSMLPP ENSLLPS LLQESGGIPWKAMIGLIKKKS WRLSTIPLL
Sbjct: 61  SSSGGDGGSLTVAVNSMLPPAENSLLPSVLLQESGGIPWKAMIGLIKKKSAWRLSTIPLL 120

Query: 201 AMSYDISKKNFSKRFARIRSTDEGISIEDVPFCKPSWRIYGFSELATATNNFSPENLLGK 260
           AMSYDISKKNFSKRFARIRSTDEGISIEDVPFCKPSWRIYGFSELATATNNFSPENLLGK
Sbjct: 121 AMSYDISKKNFSKRFARIRSTDEGISIEDVPFCKPSWRIYGFSELATATNNFSPENLLGK 180

Query: 261 GGQAEVYRGCLSDGQFVAVKRLMKREKDNEEKTSDFLTELGIIAHISHPNAARLLGFGID 320
           GGQAEVYRGCLSDGQFVAVKRLMKREKDNEEK SDFLTELGIIAHISHPNAARLLGFGID
Sbjct: 181 GGQAEVYRGCLSDGQFVAVKRLMKREKDNEEKASDFLTELGIIAHISHPNAARLLGFGID 240

Query: 321 GGLYLVLEYIPFGSFASALFGAESLDWKIRFKVAIGVADGLNYLHRECPRRIIHRDIKAS 380
           GGLYLVLEYIPFGSFASALFGAESLDWKIRFKVAIGVADGLNYLHRECPRRIIHRDIKAS
Sbjct: 241 GGLYLVLEYIPFGSFASALFGAESLDWKIRFKVAIGVADGLNYLHRECPRRIIHRDIKAS 300

Query: 381 NILLGENYEPLISDFGLAKWLPENWAHHVVYPIEGTFGYLAPEYFMHGIVDEKTDVFAYG 440
           NILLGENYEPLISDFGLAKWLPENWAHHVVYPIEGTFGYLAPEYFMHGIVDEKTDVFAYG
Sbjct: 301 NILLGENYEPLISDFGLAKWLPENWAHHVVYPIEGTFGYLAPEYFMHGIVDEKTDVFAYG 360

Query: 441 VLLLELITGRRAVDSSQQSLVIWAKPLLDNNSFQELMDPKLGDDYDQTEMRRTMLAASMC 500
           VLLLELITGRRAVDSSQQSLVIWAKPLLDNNSFQELMDPKLGDDYDQTEMRRTMLAASMC
Sbjct: 361 VLLLELITGRRAVDSSQQSLVIWAKPLLDNNSFQELMDPKLGDDYDQTEMRRTMLAASMC 420

Query: 501 INQSSSMRPHMNSVVQLLKGEEGALEQLKQNAMDEDQSCASEASNMEDYSCNSNYLDDMS 560
           IN SSSMRPHMNSVVQLLKG EGALEQLKQNAMDEDQSC SEA+NMEDYSCNSNYLDDMS
Sbjct: 421 INHSSSMRPHMNSVVQLLKG-EGALEQLKQNAMDEDQSCVSEANNMEDYSCNSNYLDDMS 480

Query: 561 RHRELILE 569
           RHRELILE
Sbjct: 481 RHRELILE 487

BLAST of Carg14258 vs. ExPASy TrEMBL
Match: A0A5A7UZG2 (Receptor-like cytosolic serine/threonine-protein kinase RBK1 OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold22G002440 PE=4 SV=1)

HSP 1 Score: 781.9 bits (2018), Expect = 1.8e-222
Identity = 386/484 (79.75%), Postives = 431/484 (89.05%), Query Frame = 0

Query: 86  EMEFGNARKGIKQMEEM-EGYGGLKIIGEEEDLCEENSPRGVFEIPVLGTDSDQSESSSG 145
           EME G   K  K+MEE  EG  G+K+ GEEE +C+ENSPRGVFEIP++GTDSDQSESSSG
Sbjct: 18  EMEIGEIGKRKKEMEETEEGEEGMKVKGEEEFVCDENSPRGVFEIPIVGTDSDQSESSSG 77

Query: 146 GDGGSLTVAVNSMLPPPENSLLPSALLQESGGIPWKAMIGLIKKKSVWRLSTIPLLAMSY 205
           G G      V   L  PE +LLP+ALLQE GGIPWKAMIG IKKKSVWR STIPLLA SY
Sbjct: 78  GGGD-----VEGTLSVPEKALLPAALLQEVGGIPWKAMIGCIKKKSVWRFSTIPLLAASY 137

Query: 206 DISKKNFSKRFARIRSTDEGISIEDVPFCKPSWRIYGFSELATATNNFSPENLLGKGGQA 265
           +IS+KNF ++ ARIRS DEGI+ +D+PFCKPSWR YGFSEL+ ATNNFSPENLLGKGGQA
Sbjct: 138 EISRKNFKRKLARIRSADEGITSDDIPFCKPSWRNYGFSELSAATNNFSPENLLGKGGQA 197

Query: 266 EVYRGCLSDGQFVAVKRLMKREKDNEEKTSDFLTELGIIAHISHPNAARLLGFGIDGGLY 325
           EVY+GCLSDGQ VAVKRLMK+EK+NEE+T+DFL+ELGIIAHI+HPNAARLLGFGI+ GLY
Sbjct: 198 EVYKGCLSDGQIVAVKRLMKKEKENEERTADFLSELGIIAHINHPNAARLLGFGIESGLY 257

Query: 326 LVLEYIPFGSFASALFGAESLDWKIRFKVAIGVADGLNYLHRECPRRIIHRDIKASNILL 385
           LVLE+IP GSFASALFGAESL+W IRFKVA+GVA+GLNYLH ECPRRIIHRDIKASNILL
Sbjct: 258 LVLEFIPHGSFASALFGAESLEWNIRFKVALGVAEGLNYLHCECPRRIIHRDIKASNILL 317

Query: 386 GENYEPLISDFGLAKWLPENWAHHVVYPIEGTFGYLAPEYFMHGIVDEKTDVFAYGVLLL 445
            ENYEPLISDFGLAKWLPENWAHHVV+PIEGTFGYLAPEYFMHGIVDEKTDVFA+GVLLL
Sbjct: 318 AENYEPLISDFGLAKWLPENWAHHVVFPIEGTFGYLAPEYFMHGIVDEKTDVFAFGVLLL 377

Query: 446 ELITGRRAVDSSQQSLVIWAKPLLDNNSFQELMDPKLGDDYDQTEMRRTMLAASMCINQS 505
           ELITGRRAVDSS+QSLVIWAKPLLDNNSF+EL DPKLGDDYDQTEMRRTMLAASMCIN S
Sbjct: 378 ELITGRRAVDSSRQSLVIWAKPLLDNNSFKELTDPKLGDDYDQTEMRRTMLAASMCINHS 437

Query: 506 SSMRPHMNSVVQLLKGEEGALEQLKQNAMDEDQSCASEASNMEDYSCNSNYLDDMSRHRE 565
           SSMRPHMN VVQLLKGE+G L+QLK  +MD D+S   EA ++EDY+ N++YL+D+SRHRE
Sbjct: 438 SSMRPHMNRVVQLLKGEDGPLDQLKHKSMDGDRSFLLEACDLEDYTNNTSYLNDLSRHRE 496

Query: 566 LILE 569
           L+LE
Sbjct: 498 LVLE 496

BLAST of Carg14258 vs. ExPASy TrEMBL
Match: A0A5D3D4P2 (Receptor-like cytosolic serine/threonine-protein kinase RBK1 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold119G00030 PE=4 SV=1)

HSP 1 Score: 780.0 bits (2013), Expect = 6.7e-222
Identity = 385/483 (79.71%), Postives = 430/483 (89.03%), Query Frame = 0

Query: 87  MEFGNARKGIKQMEEM-EGYGGLKIIGEEEDLCEENSPRGVFEIPVLGTDSDQSESSSGG 146
           ME G   K  K+MEE  EG  G+K+ GEEE +C+ENSPRGVFEIP++GTDSDQSESSSGG
Sbjct: 1   MEIGEIGKRKKEMEETEEGEEGMKVKGEEEFVCDENSPRGVFEIPIVGTDSDQSESSSGG 60

Query: 147 DGGSLTVAVNSMLPPPENSLLPSALLQESGGIPWKAMIGLIKKKSVWRLSTIPLLAMSYD 206
            G      V   L  PE +LLP+ALLQE GGIPWKAMIG IKKKSVWR STIPLLA SY+
Sbjct: 61  GGD-----VEGTLSVPEKALLPAALLQEVGGIPWKAMIGCIKKKSVWRFSTIPLLAASYE 120

Query: 207 ISKKNFSKRFARIRSTDEGISIEDVPFCKPSWRIYGFSELATATNNFSPENLLGKGGQAE 266
           IS+KNF ++ ARIRS DEGI+ +D+PFCKPSWR YGFSEL+ ATNNFSPENLLGKGGQAE
Sbjct: 121 ISRKNFKRKLARIRSADEGITSDDIPFCKPSWRNYGFSELSAATNNFSPENLLGKGGQAE 180

Query: 267 VYRGCLSDGQFVAVKRLMKREKDNEEKTSDFLTELGIIAHISHPNAARLLGFGIDGGLYL 326
           VY+GCLSDGQ VAVKRLMK+EK+NEE+T+DFL+ELGIIAHI+HPNAARLLGFGI+ GLYL
Sbjct: 181 VYKGCLSDGQIVAVKRLMKKEKENEERTADFLSELGIIAHINHPNAARLLGFGIESGLYL 240

Query: 327 VLEYIPFGSFASALFGAESLDWKIRFKVAIGVADGLNYLHRECPRRIIHRDIKASNILLG 386
           VLE+IP GSFASALFGAESL+W IRFKVA+GVA+GLNYLH ECPRRIIHRDIKASNILL 
Sbjct: 241 VLEFIPHGSFASALFGAESLEWNIRFKVALGVAEGLNYLHCECPRRIIHRDIKASNILLA 300

Query: 387 ENYEPLISDFGLAKWLPENWAHHVVYPIEGTFGYLAPEYFMHGIVDEKTDVFAYGVLLLE 446
           ENYEPLISDFGLAKWLPENWAHHVV+PIEGTFGYLAPEYFMHGIVDEKTDVFA+GVLLLE
Sbjct: 301 ENYEPLISDFGLAKWLPENWAHHVVFPIEGTFGYLAPEYFMHGIVDEKTDVFAFGVLLLE 360

Query: 447 LITGRRAVDSSQQSLVIWAKPLLDNNSFQELMDPKLGDDYDQTEMRRTMLAASMCINQSS 506
           LITGRRAVDSS+QSLVIWAKPLLDNNSF+EL DPKLGDDYDQTEMRRTMLAASMCIN SS
Sbjct: 361 LITGRRAVDSSRQSLVIWAKPLLDNNSFKELTDPKLGDDYDQTEMRRTMLAASMCINHSS 420

Query: 507 SMRPHMNSVVQLLKGEEGALEQLKQNAMDEDQSCASEASNMEDYSCNSNYLDDMSRHREL 566
           SMRPHMN VVQLLKGE+G L+QLK  +MD D+S   EA ++EDY+ N++YL+D+SRHREL
Sbjct: 421 SMRPHMNRVVQLLKGEDGPLDQLKHKSMDGDRSFLLEACDLEDYTNNTSYLNDLSRHREL 478

Query: 567 ILE 569
           +LE
Sbjct: 481 VLE 478

BLAST of Carg14258 vs. ExPASy TrEMBL
Match: A0A0A0L2T4 (Protein kinase domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_3G114500 PE=4 SV=1)

HSP 1 Score: 774.6 bits (1999), Expect = 2.8e-220
Identity = 387/499 (77.56%), Postives = 437/499 (87.58%), Query Frame = 0

Query: 76  DQILKMKVEMEMEFGNARKGIKQMEEM-EGYGGLKIIGEEEDLCEENSPRGVFEIPVLGT 135
           DQIL    + EME G   K  K+MEE  EG  G+K+ GEEE +C+ENSPRGVFEIP++GT
Sbjct: 12  DQIL----QPEMEIGEIGKRKKEMEETEEGEEGMKVKGEEEFVCDENSPRGVFEIPIVGT 71

Query: 136 DSDQSESSSGGDG-----GSLTVAVNSMLPPPENSLLPSALLQESGGIPWKAMIGLIKKK 195
           DSDQSESSSG  G     G+LTVA        E +LLP ALLQE GGIPWKAMIG IKKK
Sbjct: 72  DSDQSESSSGDGGGEDGEGTLTVA--------EKALLPGALLQEVGGIPWKAMIGCIKKK 131

Query: 196 SVWRLSTIPLLAMSYDISKKNFSKRFARIRSTDEGISIEDVPFCKPSWRIYGFSELATAT 255
           SVWR STIPLLA SY+IS+KNF ++ ARIRS DEGI+ +D+PFCKPSWR YGF+EL+ AT
Sbjct: 132 SVWRFSTIPLLAASYEISRKNFKRKLARIRSADEGITGDDIPFCKPSWRNYGFAELSAAT 191

Query: 256 NNFSPENLLGKGGQAEVYRGCLSDGQFVAVKRLMKREKDNEEKTSDFLTELGIIAHISHP 315
           NNFSPENLLGKGGQAEVY+GCLSDGQ VAVKRLMK+EK+NEE+T+DFL+ELGIIAHI+HP
Sbjct: 192 NNFSPENLLGKGGQAEVYKGCLSDGQIVAVKRLMKKEKENEERTADFLSELGIIAHINHP 251

Query: 316 NAARLLGFGIDGGLYLVLEYIPFGSFASALFGAESLDWKIRFKVAIGVADGLNYLHRECP 375
           NAARLLGFGI+ GLYLVLE+IP+GSFASALFGAE L+W IRFKVA+GVA+GLNYLHRECP
Sbjct: 252 NAARLLGFGIESGLYLVLEFIPYGSFASALFGAEPLEWNIRFKVALGVAEGLNYLHRECP 311

Query: 376 RRIIHRDIKASNILLGENYEPLISDFGLAKWLPENWAHHVVYPIEGTFGYLAPEYFMHGI 435
           RRIIHRDIKASNILL ENYEPLISDFGLAKWLPENWAHHVV+PIEGTFGYLAPEYFMHGI
Sbjct: 312 RRIIHRDIKASNILLAENYEPLISDFGLAKWLPENWAHHVVFPIEGTFGYLAPEYFMHGI 371

Query: 436 VDEKTDVFAYGVLLLELITGRRAVDSSQQSLVIWAKPLLDNNSFQELMDPKLGDDYDQTE 495
           VDE+TDVFA+GVLLLELITGRRAVDSS+QSLVIWAKPLLDNNSF+EL DPKLGD+YDQTE
Sbjct: 372 VDERTDVFAFGVLLLELITGRRAVDSSRQSLVIWAKPLLDNNSFKELTDPKLGDNYDQTE 431

Query: 496 MRRTMLAASMCINQSSSMRPHMNSVVQLLKGEEGALEQLKQNAMDEDQSCASEASNMEDY 555
           M RTMLAASMCIN SSSMRPHMN VVQLLKGE+G L+QLK  +MD D+S   EA ++EDY
Sbjct: 432 MGRTMLAASMCINHSSSMRPHMNRVVQLLKGEDGPLDQLKHKSMDGDRSLLLEACDLEDY 491

Query: 556 SCNSNYLDDMSRHRELILE 569
           + NS+YL+D++RHREL+LE
Sbjct: 492 TNNSSYLNDLNRHRELVLE 498

BLAST of Carg14258 vs. TAIR 10
Match: AT5G65530.1 (Protein kinase superfamily protein )

HSP 1 Score: 488.8 bits (1257), Expect = 5.9e-138
Identity = 262/479 (54.70%), Postives = 341/479 (71.19%), Query Frame = 0

Query: 99  MEEMEGYGGLKIIGEEE------DL-CEENSPRGVFEIPVLGTDSDQSESSSGGDGGSLT 158
           +EEME     KI G +E      DL  E+ SPR V EIPV+ +DSD S S S        
Sbjct: 3   VEEME----KKIKGSQETELHKNDLSIEDPSPRCVLEIPVMSSDSDNSSSCS-------- 62

Query: 159 VAVNSMLPPPENSLLPSALLQESGGIPWKAMIGLIKKKSVWRLSTIPLLAMSYDISKKNF 218
               S  P   +S L +     S    W  MI  IKKKS+ R S IPLLA SY++++K  
Sbjct: 63  ----SCSPDKSSSPLSTTPPNVSSFHQWNKMIESIKKKSIRRFSVIPLLA-SYELTRKKK 122

Query: 219 SKRFARIRSTDEGISIEDVPFCKPSWRIYGFSELATATNNFSPENLLGKGGQAEVYRGCL 278
             + +     D     + +   KPSWR + F EL  AT+NF+PEN++GKGG AEVY+G L
Sbjct: 123 QPKLSPCSEND--FDCDQILVAKPSWRNFTFDELVAATDNFNPENMIGKGGHAEVYKGVL 182

Query: 279 SDGQFVAVKRLMKREKDNEEKTSDFLTELGIIAHISHPNAARLLGFGIDGGLYLVLEYIP 338
            DG+ VA+K+L +  K+ EE+ SDFL+ELGIIAH++HPNAARL GF  D GL+ VLEY  
Sbjct: 183 PDGETVAIKKLTRHAKEVEERVSDFLSELGIIAHVNHPNAARLRGFSCDRGLHFVLEYSS 242

Query: 339 FGSFASALFGAES-LDWKIRFKVAIGVADGLNYLHRECPRRIIHRDIKASNILLGENYEP 398
            GS AS LFG+E  LDWK R+KVA+G+ADGL+YLH +CPRRIIHRDIKASNILL ++YE 
Sbjct: 243 HGSLASLLFGSEECLDWKKRYKVAMGIADGLSYLHNDCPRRIIHRDIKASNILLSQDYEA 302

Query: 399 LISDFGLAKWLPENWAHHVVYPIEGTFGYLAPEYFMHGIVDEKTDVFAYGVLLLELITGR 458
            ISDFGLAKWLPE+W HH+V+PIEGTFGYLAPEYFMHGIVDEKTDVFA+GVLLLE+ITGR
Sbjct: 303 QISDFGLAKWLPEHWPHHIVFPIEGTFGYLAPEYFMHGIVDEKTDVFAFGVLLLEIITGR 362

Query: 459 RAVDS-SQQSLVIWAKPLLDNNSFQELMDPKLGDDYDQTEMRRTMLAASMCINQSSSMRP 518
           RAVD+ S+QS+V+WAKPLL+ N+ +E++DP+LG+D+D+TEM+R M  ASMCI+  S+MRP
Sbjct: 363 RAVDTDSRQSIVMWAKPLLEKNNMEEIVDPQLGNDFDETEMKRVMQTASMCIHHVSTMRP 422

Query: 519 HMNSVVQLLKGEEGALEQLKQNAMDEDQSCASEASNMEDYSCNSNYLDDMSRHRELILE 569
            MN +VQLL+G++   EQ    A    ++ + +  +++  S  S+YL+D++RHR+L++E
Sbjct: 423 DMNRLVQLLRGDDQLAEQKPGGA----RTVSLDDCDLDHTS--SSYLNDLTRHRQLLME 456

BLAST of Carg14258 vs. TAIR 10
Match: AT5G10520.1 (ROP binding protein kinases 1 )

HSP 1 Score: 483.0 bits (1242), Expect = 3.2e-136
Identity = 249/452 (55.09%), Postives = 330/452 (73.01%), Query Frame = 0

Query: 119 EENSPRGVFEIPVLGTDSDQSESSSGGDGGSLTVAVNSMLPPPENSLLPSALLQESGGIP 178
           + +SPRG     VLG  SD   SSS     S     +S   P  N+     +     G+ 
Sbjct: 28  DSSSPRG-----VLGMVSDSDNSSSSCSSCSSDDKSSSTSSPFSNT--TKTVSSSHHGLQ 87

Query: 179 WKAMIGLIKKKSVWRLSTIPLLAMSYDISKKNFSKRFARIRSTDEGISIEDVPFCKPSWR 238
           W  MI  IKKKS+ R S IPLLA SY++++KN  ++  ++  ++   + E     KPSWR
Sbjct: 88  WNKMIESIKKKSMRRFSVIPLLA-SYELTRKNLRRKQPKLTPSESAFTCEAFFMAKPSWR 147

Query: 239 IYGFSELATATNNFSPENLLGKGGQAEVYRGCLSDGQFVAVKRLMKREKDNEEKTSDFLT 298
            + + ELA AT+ F+PEN++GKGG AEVY+G L +G+ VA+K+LM   K+ EE+ SDFL+
Sbjct: 148 NFTYEELAVATDYFNPENMIGKGGHAEVYKGVLINGETVAIKKLMSHAKEEEERVSDFLS 207

Query: 299 ELGIIAHISHPNAARLLGFGIDGGLYLVLEYIPFGSFASALFGAES-LDWKIRFKVAIGV 358
           ELGIIAH++HPNAARL GF  D GL+ VLEY P+GS AS LFG+E  L+WKIR+KVA+G+
Sbjct: 208 ELGIIAHVNHPNAARLRGFSSDRGLHFVLEYAPYGSLASMLFGSEECLEWKIRYKVALGI 267

Query: 359 ADGLNYLHRECPRRIIHRDIKASNILLGENYEPLISDFGLAKWLPENWAHHVVYPIEGTF 418
           ADGL+YLH  CPRRIIHRDIKASNILL  +YE  ISDFGLAKWLPENW HHVV+PIEGTF
Sbjct: 268 ADGLSYLHNACPRRIIHRDIKASNILLNHDYEAQISDFGLAKWLPENWPHHVVFPIEGTF 327

Query: 419 GYLAPEYFMHGIVDEKTDVFAYGVLLLELITGRRAVD-SSQQSLVIWAKPLLDNNSFQEL 478
           GYLAPEYFMHGIVDEK DVFA+GVLLLE+IT RRAVD +S+QS+V WAKP L+ NS +++
Sbjct: 328 GYLAPEYFMHGIVDEKIDVFAFGVLLLEIITSRRAVDTASRQSIVAWAKPFLEKNSMEDI 387

Query: 479 MDPKLGDDYDQTEMRRTMLAASMCINQSSSMRPHMNSVVQLLKGEEGALEQLKQNAMDED 538
           +DP+LG+ ++ TEM+R ML ASMC++  ++MRP M  +VQLL+GE+G  E L+Q A +  
Sbjct: 388 VDPRLGNMFNPTEMQRVMLTASMCVHHIAAMRPDMTRLVQLLRGEDGPAE-LQQKAGERT 447

Query: 539 QSCASEASNMEDYSCNSNYLDDMSRHRELILE 569
            S    A +++D++ +S+YL+++ RHR+L++E
Sbjct: 448 MSV--NACDLQDHT-SSSYLNELRRHRQLLME 467

BLAST of Carg14258 vs. TAIR 10
Match: AT5G18910.1 (Protein kinase superfamily protein )

HSP 1 Score: 389.0 bits (998), Expect = 6.4e-108
Identity = 186/338 (55.03%), Postives = 260/338 (76.92%), Query Frame = 0

Query: 232 FCKPSWRIYGFSELATATNNFSPENLLGKGGQAEVYRGCLSDGQFVAVKRLMKREKDNEE 291
           + KPSWR +   ++ TATN++S ENL+G+GG AEVY+G ++DGQ VA+K+L +     EE
Sbjct: 172 YFKPSWRNFSLRDIQTATNDYSRENLIGEGGYAEVYKGQMADGQIVAIKKLTRGSA--EE 231

Query: 292 KTSDFLTELGIIAHISHPNAARLLGFGIDGGLYLVLEYIPFGSFASALFGA-ESLDWKIR 351
            T D+L+ELGII H+ HPN A+L+G+ ++GG++LVLE  P GS AS L+ A E L+W +R
Sbjct: 232 MTMDYLSELGIIVHVDHPNIAKLIGYCVEGGMHLVLELSPNGSLASLLYEAKEKLNWSMR 291

Query: 352 FKVAIGVADGLNYLHRECPRRIIHRDIKASNILLGENYEPLISDFGLAKWLPENWAHHVV 411
           +KVA+G A+GL YLH  C RRIIH+DIKASNILL +N+E  ISDFGLAKWLP+ W HH V
Sbjct: 292 YKVAMGTAEGLYYLHEGCQRRIIHKDIKASNILLTQNFEAQISDFGLAKWLPDQWTHHTV 351

Query: 412 YPIEGTFGYLAPEYFMHGIVDEKTDVFAYGVLLLELITGRRAVDSSQQSLVIWAKPLLDN 471
             +EGTFGYL PE+FMHGIVDEKTDV+AYGVLLLELITGR+A+DSSQ S+V+WAKPL+  
Sbjct: 352 SKVEGTFGYLPPEFFMHGIVDEKTDVYAYGVLLLELITGRQALDSSQHSIVMWAKPLIKE 411

Query: 472 NSFQELMDPKLGDDYDQTEMRRTMLAASMCINQSSSMRPHMNSVVQLLKGEEGALEQLKQ 531
           N  ++L+DP L DDYD  E+ R +  AS+CI+Q+S  RP M+ VV++L+G++ +L++L++
Sbjct: 412 NKIKQLVDPILEDDYDVEELDRLVFIASLCIHQTSMNRPQMSQVVEILRGDKCSLDKLRE 471

Query: 532 NAMDE-DQSCASEASNMEDYSCNSNYLDDMSRHRELIL 568
               +  ++ + E  + E+Y+ ++ YL+D++RH E +L
Sbjct: 472 RENSKLQRTYSEELLDNEEYN-STRYLNDINRHMETVL 506

BLAST of Carg14258 vs. TAIR 10
Match: AT5G35960.1 (Protein kinase family protein )

HSP 1 Score: 377.5 bits (968), Expect = 1.9e-104
Identity = 187/343 (54.52%), Postives = 251/343 (73.18%), Query Frame = 0

Query: 179 WKAMIGLIKKKSVWRLSTIPLLAMSYDISKKNFSKRFARIRSTDEGISIEDVPFCKPSWR 238
           ++ M+ L++ KS   L  + +   +  + K +F +R + +R      S +         R
Sbjct: 62  FRKMVQLLRSKSKKSLENVKIPFHNNGVIKSSF-RRCSSMRENLRFSSNDSHFLLHSPRR 121

Query: 239 IYGFSELATATNNFSPENLLGKGGQAEVYRGCLSDGQFVAVKRLMKREKDNEEKTSDFLT 298
           I+ FS+L +ATNNFS ENL+GKGG AEVY+G L +GQ VA+KRLM+   ++EE   DFL+
Sbjct: 122 IFTFSDLKSATNNFSLENLIGKGGYAEVYKGMLPNGQMVAIKRLMR--GNSEEIIVDFLS 181

Query: 299 ELGIIAHISHPNAARLLGFGIDGGLYLVLEYIPFGSFASALFGA-ESLDWKIRFKVAIGV 358
           E+GI+AH++HPN A+LLG+G++GG++LVLE  P GS AS L+ + E + W IR+K+A+GV
Sbjct: 182 EMGIMAHVNHPNIAKLLGYGVEGGMHLVLELSPHGSLASMLYSSKEKMKWSIRYKIALGV 241

Query: 359 ADGLNYLHRECPRRIIHRDIKASNILLGENYEPLISDFGLAKWLPENWAHHVVYPIEGTF 418
           A+GL YLHR C RRIIHRDIKA+NILL  ++ P I DFGLAKWLPENW HH+V   EGTF
Sbjct: 242 AEGLVYLHRGCHRRIIHRDIKAANILLTHDFSPQICDFGLAKWLPENWTHHIVSKFEGTF 301

Query: 419 GYLAPEYFMHGIVDEKTDVFAYGVLLLELITGRRAVDSSQQSLVIWAKPLLDNNSFQELM 478
           GYLAPEY  HGIVDEKTDVFA GVLLLEL+TGRRA+D S+QSLV+WAKPL+  N  +EL+
Sbjct: 302 GYLAPEYLTHGIVDEKTDVFALGVLLLELVTGRRALDYSKQSLVLWAKPLMKKNKIRELI 361

Query: 479 DPKLGDDYDQTEMRRTMLAASMCINQSSSMRPHMNSVVQLLKG 521
           DP L  +Y+  +++  +LAA++ I QSS  RP M+ VV++LKG
Sbjct: 362 DPSLAGEYEWRQIKLVLLAAALSIQQSSIERPEMSQVVEILKG 401

BLAST of Carg14258 vs. TAIR 10
Match: AT3G05140.1 (ROP binding protein kinases 2 )

HSP 1 Score: 368.2 bits (944), Expect = 1.2e-101
Identity = 215/474 (45.36%), Postives = 288/474 (60.76%), Query Frame = 0

Query: 108 LKIIGEEEDLCEENSPRGVFEIPVLGTDSDQSESSSGGDGGSLTVAVNSMLPPPENSLLP 167
           L+++  +++  +  SPRG  E   L   S  S SSS  D            PPP    + 
Sbjct: 9   LRLLEVDKEKQDPKSPRGALE-ACLTRCSISSASSSSDD------------PPPNREAID 68

Query: 168 SA-------LLQESGGIPWKAMIGLIKKKSVWRLSTI-PLLAMSYDISKKNFSKRFARIR 227
           +A                W     L K++S+ RLS+  PL   +  I K+N        +
Sbjct: 69  NADADTDVQCKNHRASSNWGKFFKLWKRRSMKRLSSFPPLSGAAPPIIKQN--------K 128

Query: 228 STD---EGISIEDVPFCKPSWRIYGFSELATATNNFSPENLLGKGGQAEVYRGCLSDGQF 287
           S D    G+ + D+   + S + +  S++  AT+NFSPEN++G+GG A+VY+G L +G+ 
Sbjct: 129 SADPNMNGMVLHDIYDFQSSLQNFSISDIEIATDNFSPENIIGRGGYADVYQGILPEGKL 188

Query: 288 VAVKRLMKREKDNEEKTSDFLTELGIIAHISHPNAARLLGFGIDGGLYLVLEYIPFGSFA 347
           +AVKRL K   D  E+T++FL+ELGIIAH+ HPN A+ +G  I+GG++LV    P GS  
Sbjct: 189 IAVKRLTKGTPD--EQTAEFLSELGIIAHVDHPNTAKFIGCCIEGGMHLVFRLSPLGSLG 248

Query: 348 SALFGAE--SLDWKIRFKVAIGVADGLNYLHRECPRRIIHRDIKASNILLGENYEPLISD 407
           S L G     L W  R+ VA+G ADGL YLH  C RRIIHRDIKA NILL E+++P I D
Sbjct: 249 SLLHGPSKYKLTWSRRYNVALGTADGLVYLHEGCQRRIIHRDIKADNILLTEDFQPQICD 308

Query: 408 FGLAKWLPENWAHHVVYPIEGTFGYLAPEYFMHGIVDEKTDVFAYGVLLLELITGRRAVD 467
           FGLAKWLP+   HH V   EGTFGY APEYFMHGIVDEKTDVFA+GVLLLELITG  A+D
Sbjct: 309 FGLAKWLPKQLTHHNVSKFEGTFGYFAPEYFMHGIVDEKTDVFAFGVLLLELITGHPALD 368

Query: 468 SSQQSLVIWAKPLLDNNSFQELMDPKLGDDYDQTEMRRTMLAASMCINQSSSMRPHMNSV 527
            SQQSLV+WAKPLL+  + +EL+DP LGD+Y++ E+ R    AS+CI+QSS +RP M+ V
Sbjct: 369 ESQQSLVLWAKPLLERKAIKELVDPSLGDEYNREELIRLTSTASLCIDQSSLLRPRMSQV 428

Query: 528 VQLLKGEEGALEQLKQNAMDEDQSCASEASNMEDYSCNS-NYLDDMSRHRELIL 568
           V+LL G E  +   ++  +   Q   SE   ++    NS  YL D+ R RE+ L
Sbjct: 429 VELLLGHEDVVMTPREAKIKMMQRTYSE-ELLDSVEYNSTKYLGDLDRIREVAL 458

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
KAG7031325.10.0e+00100.00Receptor-like cytosolic serine/threonine-protein kinase RBK1, partial [Cucurbita... [more]
KAG6600690.12.1e-29496.10Receptor-like cytosolic serine/threonine-protein kinase RBK1, partial [Cucurbita... [more]
XP_023548346.11.3e-28099.39receptor-like cytosolic serine/threonine-protein kinase RBK1 [Cucurbita pepo sub... [more]
XP_022941642.12.9e-28099.18receptor-like cytosolic serine/threonine-protein kinase RBK1 [Cucurbita moschata... [more]
XP_022992501.14.8e-27597.95receptor-like cytosolic serine/threonine-protein kinase RBK1 [Cucurbita maxima] ... [more]
Match NameE-valueIdentityDescription
Q8H1D64.6e-13555.09Receptor-like cytosolic serine/threonine-protein kinase RBK1 OS=Arabidopsis thal... [more]
Q8RXC81.6e-10045.36Receptor-like cytosolic serine/threonine-protein kinase RBK2 OS=Arabidopsis thal... [more]
Q5XF574.5e-9050.60Probable receptor-like serine/threonine-protein kinase At5g57670 OS=Arabidopsis ... [more]
Q8RWW05.1e-6239.39Receptor-like serine/threonine-protein kinase ALE2 OS=Arabidopsis thaliana OX=37... [more]
Q9LV483.3e-6139.40Proline-rich receptor-like protein kinase PERK1 OS=Arabidopsis thaliana OX=3702 ... [more]
Match NameE-valueIdentityDescription
A0A6J1FN211.4e-28099.18receptor-like cytosolic serine/threonine-protein kinase RBK1 OS=Cucurbita moscha... [more]
A0A6J1JTS62.3e-27597.95receptor-like cytosolic serine/threonine-protein kinase RBK1 OS=Cucurbita maxima... [more]
A0A5A7UZG21.8e-22279.75Receptor-like cytosolic serine/threonine-protein kinase RBK1 OS=Cucumis melo var... [more]
A0A5D3D4P26.7e-22279.71Receptor-like cytosolic serine/threonine-protein kinase RBK1 OS=Cucumis melo var... [more]
A0A0A0L2T42.8e-22077.56Protein kinase domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_3G114... [more]
Match NameE-valueIdentityDescription
AT5G65530.15.9e-13854.70Protein kinase superfamily protein [more]
AT5G10520.13.2e-13655.09ROP binding protein kinases 1 [more]
AT5G18910.16.4e-10855.03Protein kinase superfamily protein [more]
AT5G35960.11.9e-10454.52Protein kinase family protein [more]
AT3G05140.11.2e-10145.36ROP binding protein kinases 2 [more]
InterPro
Analysis Name: InterPro Annotations of Silver-seed gourd (SMH-JMG-627) v2
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availableCOILSCoilCoilcoord: 511..531
NoneNo IPR availableGENE3D1.10.510.10Transferase(Phosphotransferase) domain 1coord: 333..547
e-value: 4.5E-55
score: 188.2
NoneNo IPR availableGENE3D3.30.200.20Phosphorylase Kinase; domain 1coord: 215..329
e-value: 5.8E-23
score: 82.8
NoneNo IPR availablePIRSRPIRSR000620-2PIRSR000620-2coord: 347..521
e-value: 2.9E-11
score: 40.7
NoneNo IPR availablePANTHERPTHR47987:SF7PROTEIN KINASE SUPERFAMILY PROTEINcoord: 101..567
NoneNo IPR availablePANTHERPTHR47987OS08G0249100 PROTEINcoord: 101..567
IPR000719Protein kinase domainSMARTSM00220serkin_6coord: 252..518
e-value: 2.5E-20
score: 83.5
IPR000719Protein kinase domainPFAMPF00069Pkinasecoord: 255..463
e-value: 2.3E-38
score: 132.1
IPR000719Protein kinase domainPROSITEPS50011PROTEIN_KINASE_DOMcoord: 252..525
score: 33.347065
IPR008271Serine/threonine-protein kinase, active sitePROSITEPS00108PROTEIN_KINASE_STcoord: 372..384
IPR011009Protein kinase-like domain superfamilySUPERFAMILY56112Protein kinase-like (PK-like)coord: 229..518

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Carg14258-RACarg14258-RAmRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0006468 protein phosphorylation
molecular_function GO:0005524 ATP binding
molecular_function GO:0004672 protein kinase activity