Carg14106 (gene) Silver-seed gourd (SMH-JMG-627) v2

Overview


Name	Carg14106
Type	gene
Organism	Cucurbita argyrosperma subsp. argyrosperma cv. SMH-JMG-627 (Silver-seed gourd (SMH-JMG-627) v2)
Description	Hydroxymethylbilane hydrolyase [cyclizing]
Location	Carg_Chr02: 6054879 .. 6055814 (-)
RNA-Seq Expression	Carg14106
Synteny	Carg14106

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGAGCACCGGCGCCGCCCACCCCAGCCCAATCCACCACCTAAATCCACTCGCTTCATCTCCTTCTCCCGCCATTCCCACCAACTCCCCTTCGCGTACGGTGGCGTTTACGACGCCTCAGAACTATGCCGGCAGCCTCTCGAACCTCCTCTCTCTCAAAGGCTTCGACCCCCTCTGGTGCCCCACCGTCACCGTCCACCCAACTCCCCTCGCCATCAAATCCCATCTCCTTCCTCCAAATCTCCATTTCTACTCCGCTGTCGCCTTCACCTCCCGCTCTGGCATCACAGCCCTCCTCGACGCTGCTACTGAGATCGACGAGCCCTTGCTATCGCCTCAGGGCGACACATTTCTAATCGCAGCCCTAGGTAAGGACTCGGAGCTTCTCGATCATGGAGTTCTTTCCAAATTTTGCCCTAACGCGAGCCGGATTAGAGTCGTCGTACCTAAAATAGCTTCGCCGAGTGGTCTAGTGGAGGCTCTTGGAATTGGGAACCATCGTAGGGTTCTGTGTCCGGTTCCTCGCGTCGTGGGGCTGAACGAGCCTCCGGTGGTTCCAAACTTCCTCCGCGACCTCGCGGCGAGCGGGTGGGTTCCGGTTCGTGTCGATGCATACGAGACCCGATGGGTCGGACCCGAGTGCGCGAGGAAGCTAGCGGAGAGAGGGGAGGATGAGAAATTGGATGCCATTGTGTTTACTAGTACTGGGGAAGTGGAGGGGCTGCTAAAAAGCTTGAGGGCTTTGGGATTGAAGTGGGAGATGATGAGAAAAAAGTGGCCGGAAATGGTGTTGGCGGCGCACGGTCCGGTGACGGCGGCGGGAGCTGAGAGGCTCGGCGTTAAGATTGATTTGGTGAGTTCTAAATTCGACAGCTTCAATGGTGTGGTCGATGCTCTTCATTCGAGATGGCAGAGCTTAGAACAGAACCCTGAGTAA

mRNA sequence

ATGAGCACCGGCGCCGCCCACCCCAGCCCAATCCACCACCTAAATCCACTCGCTTCATCTCCTTCTCCCGCCATTCCCACCAACTCCCCTTCGCGTACGGTGGCGTTTACGACGCCTCAGAACTATGCCGGCAGCCTCTCGAACCTCCTCTCTCTCAAAGGCTTCGACCCCCTCTGGTGCCCCACCGTCACCGTCCACCCAACTCCCCTCGCCATCAAATCCCATCTCCTTCCTCCAAATCTCCATTTCTACTCCGCTGTCGCCTTCACCTCCCGCTCTGGCATCACAGCCCTCCTCGACGCTGCTACTGAGATCGACGAGCCCTTGCTATCGCCTCAGGGCGACACATTTCTAATCGCAGCCCTAGGTAAGGACTCGGAGCTTCTCGATCATGGAGTTCTTTCCAAATTTTGCCCTAACGCGAGCCGGATTAGAGTCGTCGTACCTAAAATAGCTTCGCCGAGTGGTCTAGTGGAGGCTCTTGGAATTGGGAACCATCGTAGGGTTCTGTGTCCGGTTCCTCGCGTCGTGGGGCTGAACGAGCCTCCGGTGGTTCCAAACTTCCTCCGCGACCTCGCGGCGAGCGGGTGGGTTCCGGTTCGTGTCGATGCATACGAGACCCGATGGGTCGGACCCGAGTGCGCGAGGAAGCTAGCGGAGAGAGGGGAGGATGAGAAATTGGATGCCATTGTGTTTACTAGTACTGGGGAAGTGGAGGGGCTGCTAAAAAGCTTGAGGGCTTTGGGATTGAAGTGGGAGATGATGAGAAAAAAGTGGCCGGAAATGGTGTTGGCGGCGCACGGTCCGGTGACGGCGGCGGGAGCTGAGAGGCTCGGCGTTAAGATTGATTTGGTGAGTTCTAAATTCGACAGCTTCAATGGTGTGGTCGATGCTCTTCATTCGAGATGGCAGAGCTTAGAACAGAACCCTGAGTAA

Coding sequence (CDS)

ATGAGCACCGGCGCCGCCCACCCCAGCCCAATCCACCACCTAAATCCACTCGCTTCATCTCCTTCTCCCGCCATTCCCACCAACTCCCCTTCGCGTACGGTGGCGTTTACGACGCCTCAGAACTATGCCGGCAGCCTCTCGAACCTCCTCTCTCTCAAAGGCTTCGACCCCCTCTGGTGCCCCACCGTCACCGTCCACCCAACTCCCCTCGCCATCAAATCCCATCTCCTTCCTCCAAATCTCCATTTCTACTCCGCTGTCGCCTTCACCTCCCGCTCTGGCATCACAGCCCTCCTCGACGCTGCTACTGAGATCGACGAGCCCTTGCTATCGCCTCAGGGCGACACATTTCTAATCGCAGCCCTAGGTAAGGACTCGGAGCTTCTCGATCATGGAGTTCTTTCCAAATTTTGCCCTAACGCGAGCCGGATTAGAGTCGTCGTACCTAAAATAGCTTCGCCGAGTGGTCTAGTGGAGGCTCTTGGAATTGGGAACCATCGTAGGGTTCTGTGTCCGGTTCCTCGCGTCGTGGGGCTGAACGAGCCTCCGGTGGTTCCAAACTTCCTCCGCGACCTCGCGGCGAGCGGGTGGGTTCCGGTTCGTGTCGATGCATACGAGACCCGATGGGTCGGACCCGAGTGCGCGAGGAAGCTAGCGGAGAGAGGGGAGGATGAGAAATTGGATGCCATTGTGTTTACTAGTACTGGGGAAGTGGAGGGGCTGCTAAAAAGCTTGAGGGCTTTGGGATTGAAGTGGGAGATGATGAGAAAAAAGTGGCCGGAAATGGTGTTGGCGGCGCACGGTCCGGTGACGGCGGCGGGAGCTGAGAGGCTCGGCGTTAAGATTGATTTGGTGAGTTCTAAATTCGACAGCTTCAATGGTGTGGTCGATGCTCTTCATTCGAGATGGCAGAGCTTAGAACAGAACCCTGAGTAA

Protein sequence

MSTGAAHPSPIHHLNPLASSPSPAIPTNSPSRTVAFTTPQNYAGSLSNLLSLKGFDPLWCPTVTVHPTPLAIKSHLLPPNLHFYSAVAFTSRSGITALLDAATEIDEPLLSPQGDTFLIAALGKDSELLDHGVLSKFCPNASRIRVVVPKIASPSGLVEALGIGNHRRVLCPVPRVVGLNEPPVVPNFLRDLAASGWVPVRVDAYETRWVGPECARKLAERGEDEKLDAIVFTSTGEVEGLLKSLRALGLKWEMMRKKWPEMVLAAHGPVTAAGAERLGVKIDLVSSKFDSFNGVVDALHSRWQSLEQNPE

Homology

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BLAST of Carg14106 vs. NCBI nr
Match: KAG6587847.1 (hypothetical protein SDJN03_16412, partial [Cucurbita argyrosperma subsp. sororia] >KAG7035661.1 hypothetical protein SDJN02_02459, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 629.4 bits (1622), Expect = 1.6e-176
Identity = 311/311 (100.00%), Postives = 311/311 (100.00%), Query Frame = 0


Query: 1   MSTGAAHPSPIHHLNPLASSPSPAIPTNSPSRTVAFTTPQNYAGSLSNLLSLKGFDPLWC 60
           MSTGAAHPSPIHHLNPLASSPSPAIPTNSPSRTVAFTTPQNYAGSLSNLLSLKGFDPLWC
Sbjct: 1   MSTGAAHPSPIHHLNPLASSPSPAIPTNSPSRTVAFTTPQNYAGSLSNLLSLKGFDPLWC 60

Query: 61  PTVTVHPTPLAIKSHLLPPNLHFYSAVAFTSRSGITALLDAATEIDEPLLSPQGDTFLIA 120
           PTVTVHPTPLAIKSHLLPPNLHFYSAVAFTSRSGITALLDAATEIDEPLLSPQGDTFLIA
Sbjct: 61  PTVTVHPTPLAIKSHLLPPNLHFYSAVAFTSRSGITALLDAATEIDEPLLSPQGDTFLIA 120

Query: 121 ALGKDSELLDHGVLSKFCPNASRIRVVVPKIASPSGLVEALGIGNHRRVLCPVPRVVGLN 180
           ALGKDSELLDHGVLSKFCPNASRIRVVVPKIASPSGLVEALGIGNHRRVLCPVPRVVGLN
Sbjct: 121 ALGKDSELLDHGVLSKFCPNASRIRVVVPKIASPSGLVEALGIGNHRRVLCPVPRVVGLN 180

Query: 181 EPPVVPNFLRDLAASGWVPVRVDAYETRWVGPECARKLAERGEDEKLDAIVFTSTGEVEG 240
           EPPVVPNFLRDLAASGWVPVRVDAYETRWVGPECARKLAERGEDEKLDAIVFTSTGEVEG
Sbjct: 181 EPPVVPNFLRDLAASGWVPVRVDAYETRWVGPECARKLAERGEDEKLDAIVFTSTGEVEG 240

Query: 241 LLKSLRALGLKWEMMRKKWPEMVLAAHGPVTAAGAERLGVKIDLVSSKFDSFNGVVDALH 300
           LLKSLRALGLKWEMMRKKWPEMVLAAHGPVTAAGAERLGVKIDLVSSKFDSFNGVVDALH
Sbjct: 241 LLKSLRALGLKWEMMRKKWPEMVLAAHGPVTAAGAERLGVKIDLVSSKFDSFNGVVDALH 300

Query: 301 SRWQSLEQNPE 312
           SRWQSLEQNPE
Sbjct: 301 SRWQSLEQNPE 311

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BLAST of Carg14106 vs. NCBI nr
Match: XP_023530963.1 (uncharacterized protein LOC111793350 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 624.8 bits (1610), Expect = 4.0e-175
Identity = 307/311 (98.71%), Postives = 311/311 (100.00%), Query Frame = 0


Query: 1   MSTGAAHPSPIHHLNPLASSPSPAIPTNSPSRTVAFTTPQNYAGSLSNLLSLKGFDPLWC 60
           MSTGAAHPSPIHHLNPLASSPSPAIPTNSPSRTVAFTTPQNYAGSLSNLLSLKGFDPLWC
Sbjct: 1   MSTGAAHPSPIHHLNPLASSPSPAIPTNSPSRTVAFTTPQNYAGSLSNLLSLKGFDPLWC 60

Query: 61  PTVTVHPTPLAIKSHLLPPNLHFYSAVAFTSRSGITALLDAATEIDEPLLSPQGDTFLIA 120
           PTVTVHPTP+AIKSHL+PPNLHFYSAVAFTSRSGITALLDAATEIDEPLLSPQGDTFLIA
Sbjct: 61  PTVTVHPTPIAIKSHLVPPNLHFYSAVAFTSRSGITALLDAATEIDEPLLSPQGDTFLIA 120

Query: 121 ALGKDSELLDHGVLSKFCPNASRIRVVVPKIASPSGLVEALGIGNHRRVLCPVPRVVGLN 180
           ALGKDSELLDHGVLSKFCPNA+RIRVVVPKIASPSGLVEALGIGNHRRVLCPVPRVVGLN
Sbjct: 121 ALGKDSELLDHGVLSKFCPNANRIRVVVPKIASPSGLVEALGIGNHRRVLCPVPRVVGLN 180

Query: 181 EPPVVPNFLRDLAASGWVPVRVDAYETRWVGPECARKLAERGEDEKLDAIVFTSTGEVEG 240
           EPPVVPNFLRDLAASGWVPVRVDAYETRWVGPECARKLAERGEDEKLDAIVFTSTGEVEG
Sbjct: 181 EPPVVPNFLRDLAASGWVPVRVDAYETRWVGPECARKLAERGEDEKLDAIVFTSTGEVEG 240

Query: 241 LLKSLRALGLKWEMMRKKWPEMVLAAHGPVTAAGAERLGVKIDLVSSKFDSFNGVVDALH 300
           LLKSLRALGL+WEMMRKKWPEMVLAAHGPVTAAGAERLGVKIDLVSSKFDSFNGVVDALH
Sbjct: 241 LLKSLRALGLQWEMMRKKWPEMVLAAHGPVTAAGAERLGVKIDLVSSKFDSFNGVVDALH 300

Query: 301 SRWQSLEQNPE 312
           SRWQSLEQNPE
Sbjct: 301 SRWQSLEQNPE 311

[Close]

BLAST of Carg14106 vs. NCBI nr
Match: XP_022926996.1 (uncharacterized protein LOC111433956 [Cucurbita moschata])

HSP 1 Score: 621.7 bits (1602), Expect = 3.4e-174
Identity = 308/311 (99.04%), Postives = 308/311 (99.04%), Query Frame = 0


Query: 1   MSTGAAHPSPIHHLNPLASSPSPAIPTNSPSRTVAFTTPQNYAGSLSNLLSLKGFDPLWC 60
           MSTGAAHPSPI HLNPLASSPSPAI TNSPSRTVAFTTPQNYAGSLSNLLSLKGFDPLWC
Sbjct: 1   MSTGAAHPSPIRHLNPLASSPSPAISTNSPSRTVAFTTPQNYAGSLSNLLSLKGFDPLWC 60

Query: 61  PTVTVHPTPLAIKSHLLPPNLHFYSAVAFTSRSGITALLDAATEIDEPLLSPQGDTFLIA 120
           PTVTVHPTPLAIKSHLLPPNLHFYSAVAFTSRSGITALLDAATEIDEPLLSPQGDTFLIA
Sbjct: 61  PTVTVHPTPLAIKSHLLPPNLHFYSAVAFTSRSGITALLDAATEIDEPLLSPQGDTFLIA 120

Query: 121 ALGKDSELLDHGVLSKFCPNASRIRVVVPKIASPSGLVEALGIGNHRRVLCPVPRVVGLN 180
           ALGKDSELLDHGV SKFCPNASRIRVVVPKIASPSGLVEALGIGNHRRVLCPVPRVVGLN
Sbjct: 121 ALGKDSELLDHGVFSKFCPNASRIRVVVPKIASPSGLVEALGIGNHRRVLCPVPRVVGLN 180

Query: 181 EPPVVPNFLRDLAASGWVPVRVDAYETRWVGPECARKLAERGEDEKLDAIVFTSTGEVEG 240
           EPPVVPNFLRDLAASGWVPVRVDAYETRWVGPECARKLAERGEDEKLDAIVFTSTGEVEG
Sbjct: 181 EPPVVPNFLRDLAASGWVPVRVDAYETRWVGPECARKLAERGEDEKLDAIVFTSTGEVEG 240

Query: 241 LLKSLRALGLKWEMMRKKWPEMVLAAHGPVTAAGAERLGVKIDLVSSKFDSFNGVVDALH 300
           LLKSLRALGLKWEMMRKKWPEMVLAAHGPVTAAGAERLGVKIDLVSSKFDSFNGVVDALH
Sbjct: 241 LLKSLRALGLKWEMMRKKWPEMVLAAHGPVTAAGAERLGVKIDLVSSKFDSFNGVVDALH 300

Query: 301 SRWQSLEQNPE 312
           SRWQSLEQNPE
Sbjct: 301 SRWQSLEQNPE 311

[Close]

BLAST of Carg14106 vs. NCBI nr
Match: XP_023003818.1 (uncharacterized protein LOC111497286 [Cucurbita maxima])

HSP 1 Score: 605.5 bits (1560), Expect = 2.5e-169
Identity = 301/311 (96.78%), Postives = 304/311 (97.75%), Query Frame = 0


Query: 1   MSTGAAHPSPIHHLNPLASSPSPAIPTNSPSRTVAFTTPQNYAGSLSNLLSLKGFDPLWC 60
           MSTGAAHPS I HLNPLASSPSPAIPTNSPSRTVAFTTPQNYAGSLSNLLSLKGFDPLWC
Sbjct: 1   MSTGAAHPSLI-HLNPLASSPSPAIPTNSPSRTVAFTTPQNYAGSLSNLLSLKGFDPLWC 60

Query: 61  PTVTVHPTPLAIKSHLLPPNLHFYSAVAFTSRSGITALLDAATEIDEPLLSPQGDTFLIA 120
           PTVTVHPTPLAIKSHLLPPNLHFYSAVAFTSRSGITALLDAATEID+PLLSPQGDTFLIA
Sbjct: 61  PTVTVHPTPLAIKSHLLPPNLHFYSAVAFTSRSGITALLDAATEIDDPLLSPQGDTFLIA 120

Query: 121 ALGKDSELLDHGVLSKFCPNASRIRVVVPKIASPSGLVEALGIGNHRRVLCPVPRVVGLN 180
           ALGKDSELLDHGVLSKFCPN SRIRVVVPKIA+PSGLVEALGIGNHRRVLCPVPRVVGLN
Sbjct: 121 ALGKDSELLDHGVLSKFCPNTSRIRVVVPKIATPSGLVEALGIGNHRRVLCPVPRVVGLN 180

Query: 181 EPPVVPNFLRDLAASGWVPVRVDAYETRWVGPECARKLAERGEDEKLDAIVFTSTGEVEG 240
           EPPVVPNFLRDLAASGWVPVRVDAYETRW GPECAR L +RGEDEKLDAIVFTSTGEVEG
Sbjct: 181 EPPVVPNFLRDLAASGWVPVRVDAYETRWAGPECARMLVKRGEDEKLDAIVFTSTGEVEG 240

Query: 241 LLKSLRALGLKWEMMRKKWPEMVLAAHGPVTAAGAERLGVKIDLVSSKFDSFNGVVDALH 300
           LLKSLR LGLKWEMMRKKWPEMVLAAHGPVTAAGAERLGVKIDLVSSKFDSFNGVVDALH
Sbjct: 241 LLKSLRTLGLKWEMMRKKWPEMVLAAHGPVTAAGAERLGVKIDLVSSKFDSFNGVVDALH 300

Query: 301 SRWQSLEQNPE 312
           SRWQSLEQNPE
Sbjct: 301 SRWQSLEQNPE 310

[Close]

BLAST of Carg14106 vs. NCBI nr
Match: XP_038878808.1 (uncharacterized protein LOC120070948 [Benincasa hispida] >XP_038878809.1 uncharacterized protein LOC120070948 [Benincasa hispida] >XP_038878810.1 uncharacterized protein LOC120070948 [Benincasa hispida])

HSP 1 Score: 535.0 bits (1377), Expect = 4.2e-148
Identity = 264/310 (85.16%), Postives = 284/310 (91.61%), Query Frame = 0


Query: 1   MSTGAAHPSPIHHLNPLASSPSPAIPTNSPSRTVAFTTPQNYAGSLSNLLSLKGFDPLWC 60
           MSTGAAHP+   HLNPL+ SPS AIPT+S  RTVAFTTPQNYA SLS+LLSLK F+PLWC
Sbjct: 1   MSTGAAHPNRPIHLNPLSLSPSAAIPTHSTRRTVAFTTPQNYADSLSHLLSLKSFEPLWC 60

Query: 61  PTVTVHPTPLAIKSHLLPPNLHFYSAVAFTSRSGITALLDAATEIDEPLLSPQGDTFLIA 120
           PT+TVHPTPLAIKSHLLPP LH +SAVAFTSRSGITALLDAATEI EPLL  +GDTFLIA
Sbjct: 61  PTLTVHPTPLAIKSHLLPPILHSFSAVAFTSRSGITALLDAATEIGEPLLPSRGDTFLIA 120

Query: 121 ALGKDSELLDHGVLSKFCPNASRIRVVVPKIASPSGLVEALGIGNHRRVLCPVPRVVGLN 180
           ALGKDSELLDHG LS+FCPN SRIRVVVP+IA+P+GLVEALGIGN RRVLCPVPRVVGLN
Sbjct: 121 ALGKDSELLDHGFLSRFCPNTSRIRVVVPEIATPTGLVEALGIGNQRRVLCPVPRVVGLN 180

Query: 181 EPPVVPNFLRDLAASGWVPVRVDAYETRWVGPECARKLAERGEDEKLDAIVFTSTGEVEG 240
           EPPVVPNFLRDL   GWVPVRV AYETRW GP+CARKL ERG+DEKLDAIVFTSTGEVEG
Sbjct: 181 EPPVVPNFLRDLETKGWVPVRVAAYETRWAGPDCARKLVERGKDEKLDAIVFTSTGEVEG 240

Query: 241 LLKSLRALGLKWEMMRKKWPEMVLAAHGPVTAAGAERLGVKIDLVSSKFDSFNGVVDALH 300
           LLKSL  LGLKWE+MRK+WPEMV+AA+GPVTAAGAERLG+K+DLVSSKFDSFNGVVDALH
Sbjct: 241 LLKSLGHLGLKWEVMRKRWPEMVVAANGPVTAAGAERLGIKVDLVSSKFDSFNGVVDALH 300

Query: 301 SRWQSLEQNP 311
            RWQSLEQNP
Sbjct: 301 WRWQSLEQNP 310

[Close]

BLAST of Carg14106 vs. ExPASy Swiss-Prot
Match: Q8KCJ3 (Uroporphyrinogen-III synthase OS=Chlorobaculum tepidum (strain ATCC 49652 / DSM 12025 / NBRC 103806 / TLS) OX=194439 GN=hemD PE=3 SV=1)

HSP 1 Score: 55.5 bits (132), Expect = 1.3e-06
Identity = 61/266 (22.93%), Postives = 111/266 (41.73%), Query Frame = 0


Query: 32  RTVAFTTPQNYAGSLSNLLSLKGFDPLWCPTVTVHPTPLAIKSHLLPPNLHFYSAVAFTS 91
           +TV  T P++ A      L+  G D +  PT+ + P      +    P+L  ++ + FTS
Sbjct: 2   KTVLVTRPKHQAEPFVRELAQYGLDSVVFPTIEIRPV-----TGWSVPDLTRFAGIFFTS 61

Query: 92  RSGITALLDAATEIDEPLLSPQGDTFLIAALGKDS--ELLDHGVLSKFCPNASRIRVVVP 151
            + +   L+   E + P   P      + A+GK +  +L  HGV  +           +P
Sbjct: 62  PNSVQFFLERLLE-ESPDELPNLQQARVWAVGKTTGGDLEKHGVSIE----------PLP 121

Query: 152 KIASPSGLVEALGIGN-HRRVLCPVPRVVGLNEPPVVPNFLRDLAASGWVPVRVDAYETR 211
           K A    L+  +       +    V   + L   P V      +A  G + V +  Y+  
Sbjct: 122 KSADAVSLMSGIDASEIEGKTFLFVRGSLSLGTIPEV------IAKRGGICVELTVYDNI 181

Query: 212 WVGPECARKLAERGEDEKLDAIVFTSTGEVEGLLKSLRALGLKWEMMRKKWP-EMVLAAH 271
               E  +K+     + K+D + FTS        +++ +         K+ P ++++AA 
Sbjct: 182 QPSLEETQKIKSLLTEGKIDCLSFTSPSTAINFFEAIDS---------KEVPSDVLIAAI 236

Query: 272 GPVTAAGAERLGVKIDLVSSKFDSFN 294
           G  T++  E+LGVK+D++   FD  N
Sbjct: 242 GTTTSSALEKLGVKVDIIPEYFDGPN 236

[Close]

BLAST of Carg14106 vs. ExPASy TrEMBL
Match: A0A6J1EGG3 (Hydroxymethylbilane hydrolyase [cyclizing] OS=Cucurbita moschata OX=3662 GN=LOC111433956 PE=3 SV=1)

HSP 1 Score: 621.7 bits (1602), Expect = 1.7e-174
Identity = 308/311 (99.04%), Postives = 308/311 (99.04%), Query Frame = 0


Query: 1   MSTGAAHPSPIHHLNPLASSPSPAIPTNSPSRTVAFTTPQNYAGSLSNLLSLKGFDPLWC 60
           MSTGAAHPSPI HLNPLASSPSPAI TNSPSRTVAFTTPQNYAGSLSNLLSLKGFDPLWC
Sbjct: 1   MSTGAAHPSPIRHLNPLASSPSPAISTNSPSRTVAFTTPQNYAGSLSNLLSLKGFDPLWC 60

Query: 61  PTVTVHPTPLAIKSHLLPPNLHFYSAVAFTSRSGITALLDAATEIDEPLLSPQGDTFLIA 120
           PTVTVHPTPLAIKSHLLPPNLHFYSAVAFTSRSGITALLDAATEIDEPLLSPQGDTFLIA
Sbjct: 61  PTVTVHPTPLAIKSHLLPPNLHFYSAVAFTSRSGITALLDAATEIDEPLLSPQGDTFLIA 120

Query: 121 ALGKDSELLDHGVLSKFCPNASRIRVVVPKIASPSGLVEALGIGNHRRVLCPVPRVVGLN 180
           ALGKDSELLDHGV SKFCPNASRIRVVVPKIASPSGLVEALGIGNHRRVLCPVPRVVGLN
Sbjct: 121 ALGKDSELLDHGVFSKFCPNASRIRVVVPKIASPSGLVEALGIGNHRRVLCPVPRVVGLN 180

Query: 181 EPPVVPNFLRDLAASGWVPVRVDAYETRWVGPECARKLAERGEDEKLDAIVFTSTGEVEG 240
           EPPVVPNFLRDLAASGWVPVRVDAYETRWVGPECARKLAERGEDEKLDAIVFTSTGEVEG
Sbjct: 181 EPPVVPNFLRDLAASGWVPVRVDAYETRWVGPECARKLAERGEDEKLDAIVFTSTGEVEG 240

Query: 241 LLKSLRALGLKWEMMRKKWPEMVLAAHGPVTAAGAERLGVKIDLVSSKFDSFNGVVDALH 300
           LLKSLRALGLKWEMMRKKWPEMVLAAHGPVTAAGAERLGVKIDLVSSKFDSFNGVVDALH
Sbjct: 241 LLKSLRALGLKWEMMRKKWPEMVLAAHGPVTAAGAERLGVKIDLVSSKFDSFNGVVDALH 300

Query: 301 SRWQSLEQNPE 312
           SRWQSLEQNPE
Sbjct: 301 SRWQSLEQNPE 311

[Close]

BLAST of Carg14106 vs. ExPASy TrEMBL
Match: A0A6J1KXQ2 (Hydroxymethylbilane hydrolyase [cyclizing] OS=Cucurbita maxima OX=3661 GN=LOC111497286 PE=3 SV=1)

HSP 1 Score: 605.5 bits (1560), Expect = 1.2e-169
Identity = 301/311 (96.78%), Postives = 304/311 (97.75%), Query Frame = 0


Query: 1   MSTGAAHPSPIHHLNPLASSPSPAIPTNSPSRTVAFTTPQNYAGSLSNLLSLKGFDPLWC 60
           MSTGAAHPS I HLNPLASSPSPAIPTNSPSRTVAFTTPQNYAGSLSNLLSLKGFDPLWC
Sbjct: 1   MSTGAAHPSLI-HLNPLASSPSPAIPTNSPSRTVAFTTPQNYAGSLSNLLSLKGFDPLWC 60

Query: 61  PTVTVHPTPLAIKSHLLPPNLHFYSAVAFTSRSGITALLDAATEIDEPLLSPQGDTFLIA 120
           PTVTVHPTPLAIKSHLLPPNLHFYSAVAFTSRSGITALLDAATEID+PLLSPQGDTFLIA
Sbjct: 61  PTVTVHPTPLAIKSHLLPPNLHFYSAVAFTSRSGITALLDAATEIDDPLLSPQGDTFLIA 120

Query: 121 ALGKDSELLDHGVLSKFCPNASRIRVVVPKIASPSGLVEALGIGNHRRVLCPVPRVVGLN 180
           ALGKDSELLDHGVLSKFCPN SRIRVVVPKIA+PSGLVEALGIGNHRRVLCPVPRVVGLN
Sbjct: 121 ALGKDSELLDHGVLSKFCPNTSRIRVVVPKIATPSGLVEALGIGNHRRVLCPVPRVVGLN 180

Query: 181 EPPVVPNFLRDLAASGWVPVRVDAYETRWVGPECARKLAERGEDEKLDAIVFTSTGEVEG 240
           EPPVVPNFLRDLAASGWVPVRVDAYETRW GPECAR L +RGEDEKLDAIVFTSTGEVEG
Sbjct: 181 EPPVVPNFLRDLAASGWVPVRVDAYETRWAGPECARMLVKRGEDEKLDAIVFTSTGEVEG 240

Query: 241 LLKSLRALGLKWEMMRKKWPEMVLAAHGPVTAAGAERLGVKIDLVSSKFDSFNGVVDALH 300
           LLKSLR LGLKWEMMRKKWPEMVLAAHGPVTAAGAERLGVKIDLVSSKFDSFNGVVDALH
Sbjct: 241 LLKSLRTLGLKWEMMRKKWPEMVLAAHGPVTAAGAERLGVKIDLVSSKFDSFNGVVDALH 300

Query: 301 SRWQSLEQNPE 312
           SRWQSLEQNPE
Sbjct: 301 SRWQSLEQNPE 310

[Close]

BLAST of Carg14106 vs. ExPASy TrEMBL
Match: A0A5A7U8V3 (Hydroxymethylbilane hydrolyase [cyclizing] OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold675G00050 PE=3 SV=1)

HSP 1 Score: 529.6 bits (1363), Expect = 8.6e-147
Identity = 258/307 (84.04%), Postives = 281/307 (91.53%), Query Frame = 0


Query: 1   MSTGAAHPSPIHHLNPLASSPSPAIPTNSPSRTVAFTTPQNYAGSLSNLLSLKGFDPLWC 60
           MSTGAAHP+   H++PL SSPSPAIPT+S  RTVAFTTPQNYAGSLS+LLSLKGF+PLWC
Sbjct: 1   MSTGAAHPNGPFHISPLTSSPSPAIPTHSSPRTVAFTTPQNYAGSLSHLLSLKGFEPLWC 60

Query: 61  PTVTVHPTPLAIKSHLLPPNLHFYSAVAFTSRSGITALLDAATEIDEPLLSPQGDTFLIA 120
           PT+TV PTPLAIKSHLLPP LH +SAVAFTSRSGITALLDAATEI EPLL   GDTFLIA
Sbjct: 61  PTLTVQPTPLAIKSHLLPPILHSFSAVAFTSRSGITALLDAATEIGEPLLPSHGDTFLIA 120

Query: 121 ALGKDSELLDHGVLSKFCPNASRIRVVVPKIASPSGLVEALGIGNHRRVLCPVPRVVGLN 180
           ALGKDSELLDH  L+  CPN SRIRVVVP+IA+P+GLVEALG+GNHRRVLCPVPRVVGLN
Sbjct: 121 ALGKDSELLDHEFLTTICPNTSRIRVVVPEIATPTGLVEALGVGNHRRVLCPVPRVVGLN 180

Query: 181 EPPVVPNFLRDLAASGWVPVRVDAYETRWVGPECARKLAERGEDEKLDAIVFTSTGEVEG 240
           EPPVVPNFLRDL A GWVPVRVDAYETRW GP+CARKL ERG+DEKLDAIVFTSTGEVEG
Sbjct: 181 EPPVVPNFLRDLEAKGWVPVRVDAYETRWAGPDCARKLVERGKDEKLDAIVFTSTGEVEG 240

Query: 241 LLKSLRALGLKWEMMRKKWPEMVLAAHGPVTAAGAERLGVKIDLVSSKFDSFNGVVDALH 300
           LLKSL  LGL+W+MM+K+WPEMV+AAHGPVTAAGAERLGVK+DLVS KFDSFNGVVD+LH
Sbjct: 241 LLKSLEHLGLEWDMMKKRWPEMVVAAHGPVTAAGAERLGVKVDLVSPKFDSFNGVVDSLH 300

Query: 301 SRWQSLE 308
            RWQSL+
Sbjct: 301 WRWQSLD 307

[Close]

BLAST of Carg14106 vs. ExPASy TrEMBL
Match: A0A1S3BA78 (Hydroxymethylbilane hydrolyase [cyclizing] OS=Cucumis melo OX=3656 GN=LOC103487492 PE=3 SV=1)

HSP 1 Score: 529.6 bits (1363), Expect = 8.6e-147
Identity = 258/307 (84.04%), Postives = 281/307 (91.53%), Query Frame = 0


Query: 1   MSTGAAHPSPIHHLNPLASSPSPAIPTNSPSRTVAFTTPQNYAGSLSNLLSLKGFDPLWC 60
           MSTGAAHP+   H++PL SSPSPAIPT+S  RTVAFTTPQNYAGSLS+LLSLKGF+PLWC
Sbjct: 1   MSTGAAHPNGPFHISPLTSSPSPAIPTHSSPRTVAFTTPQNYAGSLSHLLSLKGFEPLWC 60

Query: 61  PTVTVHPTPLAIKSHLLPPNLHFYSAVAFTSRSGITALLDAATEIDEPLLSPQGDTFLIA 120
           PT+TV PTPLAIKSHLLPP LH +SAVAFTSRSGITALLDAATEI EPLL   GDTFLIA
Sbjct: 61  PTLTVQPTPLAIKSHLLPPILHSFSAVAFTSRSGITALLDAATEIGEPLLPSHGDTFLIA 120

Query: 121 ALGKDSELLDHGVLSKFCPNASRIRVVVPKIASPSGLVEALGIGNHRRVLCPVPRVVGLN 180
           ALGKDSELLDH  L+  CPN SRIRVVVP+IA+P+GLVEALG+GNHRRVLCPVPRVVGLN
Sbjct: 121 ALGKDSELLDHEFLTTICPNTSRIRVVVPEIATPTGLVEALGVGNHRRVLCPVPRVVGLN 180

Query: 181 EPPVVPNFLRDLAASGWVPVRVDAYETRWVGPECARKLAERGEDEKLDAIVFTSTGEVEG 240
           EPPVVPNFLRDL A GWVPVRVDAYETRW GP+CARKL ERG+DEKLDAIVFTSTGEVEG
Sbjct: 181 EPPVVPNFLRDLEAKGWVPVRVDAYETRWAGPDCARKLVERGKDEKLDAIVFTSTGEVEG 240

Query: 241 LLKSLRALGLKWEMMRKKWPEMVLAAHGPVTAAGAERLGVKIDLVSSKFDSFNGVVDALH 300
           LLKSL  LGL+W+MM+K+WPEMV+AAHGPVTAAGAERLGVK+DLVS KFDSFNGVVD+LH
Sbjct: 241 LLKSLEHLGLEWDMMKKRWPEMVVAAHGPVTAAGAERLGVKVDLVSPKFDSFNGVVDSLH 300

Query: 301 SRWQSLE 308
            RWQSL+
Sbjct: 301 WRWQSLD 307

[Close]

BLAST of Carg14106 vs. ExPASy TrEMBL
Match: A0A5D3BX13 (Hydroxymethylbilane hydrolyase [cyclizing] OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold1369G00630 PE=3 SV=1)

HSP 1 Score: 528.1 bits (1359), Expect = 2.5e-146
Identity = 257/307 (83.71%), Postives = 281/307 (91.53%), Query Frame = 0


Query: 1   MSTGAAHPSPIHHLNPLASSPSPAIPTNSPSRTVAFTTPQNYAGSLSNLLSLKGFDPLWC 60
           MSTGAAHP+   H++PL SSPSPAIPT+S  RTVAFTTPQNYAGSLS+LLSLKGF+PLWC
Sbjct: 1   MSTGAAHPNGPFHISPLTSSPSPAIPTHSSPRTVAFTTPQNYAGSLSHLLSLKGFEPLWC 60

Query: 61  PTVTVHPTPLAIKSHLLPPNLHFYSAVAFTSRSGITALLDAATEIDEPLLSPQGDTFLIA 120
           PT+TV PTPLAIKSHLLPP LH +SAVAFTSRSGITALLDAATEI EPLL   GDTFLIA
Sbjct: 61  PTLTVQPTPLAIKSHLLPPILHSFSAVAFTSRSGITALLDAATEIGEPLLPSHGDTFLIA 120

Query: 121 ALGKDSELLDHGVLSKFCPNASRIRVVVPKIASPSGLVEALGIGNHRRVLCPVPRVVGLN 180
           ALGKDSELLDH  L+  CPN SRIRVVVP+IA+P+GLVEALG+GNHRRVLCPVPRVVGLN
Sbjct: 121 ALGKDSELLDHEFLTTICPNTSRIRVVVPEIATPTGLVEALGVGNHRRVLCPVPRVVGLN 180

Query: 181 EPPVVPNFLRDLAASGWVPVRVDAYETRWVGPECARKLAERGEDEKLDAIVFTSTGEVEG 240
           EPPVVPNFLRDL A GWVPVRVDAYETRW GP+CARKL ERG+DEKLDAIVFTSTGEVEG
Sbjct: 181 EPPVVPNFLRDLEAKGWVPVRVDAYETRWAGPDCARKLVERGKDEKLDAIVFTSTGEVEG 240

Query: 241 LLKSLRALGLKWEMMRKKWPEMVLAAHGPVTAAGAERLGVKIDLVSSKFDSFNGVVDALH 300
           LLKSL  LGL+W++M+K+WPEMV+AAHGPVTAAGAERLGVK+DLVS KFDSFNGVVD+LH
Sbjct: 241 LLKSLEHLGLEWDIMKKRWPEMVVAAHGPVTAAGAERLGVKVDLVSPKFDSFNGVVDSLH 300

Query: 301 SRWQSLE 308
            RWQSL+
Sbjct: 301 WRWQSLD 307

The following BLAST results are available for this feature:

BLAST of Carg14106 vs. NCBI nr


Match Name	E-value	Identity	Description
KAG6587847.1	1.6e-176	100.00	hypothetical protein SDJN03_16412, partial [Cucurbita argyrosperma subsp. sorori...	[more]
XP_023530963.1	4.0e-175	98.71	uncharacterized protein LOC111793350 [Cucurbita pepo subsp. pepo]	[more]
XP_022926996.1	3.4e-174	99.04	uncharacterized protein LOC111433956 [Cucurbita moschata]	[more]
XP_023003818.1	2.5e-169	96.78	uncharacterized protein LOC111497286 [Cucurbita maxima]	[more]
XP_038878808.1	4.2e-148	85.16	uncharacterized protein LOC120070948 [Benincasa hispida] >XP_038878809.1 unchara...	[more]

BLAST of Carg14106 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q8KCJ3	1.3e-06	22.93	Uroporphyrinogen-III synthase OS=Chlorobaculum tepidum (strain ATCC 49652 / DSM ...	[more]

BLAST of Carg14106 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1EGG3	1.7e-174	99.04	Hydroxymethylbilane hydrolyase [cyclizing] OS=Cucurbita moschata OX=3662 GN=LOC1...	[more]
A0A6J1KXQ2	1.2e-169	96.78	Hydroxymethylbilane hydrolyase [cyclizing] OS=Cucurbita maxima OX=3661 GN=LOC111...	[more]
A0A5A7U8V3	8.6e-147	84.04	Hydroxymethylbilane hydrolyase [cyclizing] OS=Cucumis melo var. makuwa OX=119469...	[more]
A0A1S3BA78	8.6e-147	84.04	Hydroxymethylbilane hydrolyase [cyclizing] OS=Cucumis melo OX=3656 GN=LOC1034874...	[more]
A0A5D3BX13	2.5e-146	83.71	Hydroxymethylbilane hydrolyase [cyclizing] OS=Cucumis melo var. makuwa OX=119469...	[more]

BLAST of Carg14106 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description

InterPro

Analysis Name: InterPro Annotations of Silver-seed gourd (SMH-JMG-627) v2
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR036108	Tetrapyrrole biosynthesis, uroporphyrinogen III synthase superfamily	GENE3D	3.40.50.10090		coord: 28..161 e-value: 1.7E-5 score: 26.6 coord: 185..305 e-value: 2.7E-13 score: 51.8
IPR036108	Tetrapyrrole biosynthesis, uroporphyrinogen III synthase superfamily	SUPERFAMILY	69618	HemD-like	coord: 32..303
IPR003754	Tetrapyrrole biosynthesis, uroporphyrinogen III synthase	PFAM	PF02602	HEM4	coord: 45..293 e-value: 1.3E-23 score: 83.6
IPR003754	Tetrapyrrole biosynthesis, uroporphyrinogen III synthase	CDD	cd06578	HemD	coord: 34..299 e-value: 8.83813E-26 score: 100.845
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 1..31
None	No IPR available	PANTHER	PTHR38020	UROPORPHYRINOGEN-III SYNTHASE	coord: 18..305

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Carg14106-RA	Carg14106-RA	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0006782	protoporphyrinogen IX biosynthetic process
biological_process	GO:0033014	tetrapyrrole biosynthetic process
molecular_function	GO:0004852	uroporphyrinogen-III synthase activity