Carg14063 (gene) Silver-seed gourd (SMH-JMG-627) v2

Overview
NameCarg14063
Typegene
OrganismCucurbita argyrosperma subsp. argyrosperma cv. SMH-JMG-627 (Silver-seed gourd (SMH-JMG-627) v2)
DescriptionNAD(P)-binding Rossmann-fold superfamily protein
LocationCarg_Chr02: 6279737 .. 6280738 (-)
RNA-Seq ExpressionCarg14063
SyntenyCarg14063
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideexonCDSthree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGTGGATTTGGAGGTGAGAGGGACAATTAATGTTGTGGAGGCCTGTGCTCGGACAGATACTGTTGAGAAGATAGTCTTCACCTCCTCCTTGACTGCTGCCGTATGGAGAGAGAATATTCTTTCTGAGAAGGATGTGGATGAAAGGTGTTGGAGTGATAAAGAATTTTGCAGAAAAATGAAGGTTGGAAGGAATTTCAAAACCTCTGTTATCTTTTTGAGTTGGTAATTTTGGATTTCTTCATTGGGCTATGTTAATCAACGAAGCCCTTATCATTCATTTTTCTTGTCAATGAATTTGATTACAAATCAAGCTCTGTTACGTTCTCCCAGTTGTGGTATCCTCTAGCAAAGACACTCTCTGAACAAGCAGCCTGGGCTCTAGCAATGGACCGTAGGCTGAACATGGTGTCCATTAACGCAGGACTTGTTCTTGGACCCGCTGTTGCAGAAGAAAATTCAGTGTCAACAATCTCATATCTTAAAGGTAGGAGGAGAAACTTCTTTAAAGACTGTTCCACATTTGTGGGAGTACTTGTCTTGTTTTGCTTAGCTGAAAATTATGAGACAACAGGAGCCGCTGAAATGTATGAAAATGGGGTTCTGGCAGTGGTGGATGTGAAGTTTTTGGTGGATGTCCATATCCGAGCAATGGAAGATTCCTCGACGGGTGGTCGGTACTTCTGCTTCGATCAGATAGTGAATAGTGAGGATGAAACTGTTAAACTTGCGAATACATTGAGGCCATTGATATCCATTCCACCGAGGTAAAAGAATTTGGGGTTTTTAAACAACAAACAGAGCAATAACATTGGAAACTGAATCTGTGTTAGTTTATTGGTGAGAGCAGGTGCGAAGGGCAAGGGAGGGAAGGTTTTGCTGAGAGGCTGAGAAGCAGGAGATTGCACAAACTGATAGAGGGCGCAGCTTCTTGATAACTTTTGAATGTAAAAACCTTTTGGTTTTCTTGTATGCATCTCTCGCTTCCCTCTTTCTCTCTTCT

mRNA sequence

ATGGTGGATTTGGAGGTGAGAGGGACAATTAATGTTGTGGAGGCCTGTGCTCGGACAGATACTGTTGAGAAGATAGTCTTCACCTCCTCCTTGACTGCTGCCGTATGGAGAGAGAATATTCTTTCTGAGAAGGATGTGGATGAAAGCTCTGTTACGTTCTCCCAGTTGTGGTATCCTCTAGCAAAGACACTCTCTGAACAAGCAGCCTGGGCTCTAGCAATGGACCGTAGGCTGAACATGGTGTCCATTAACGCAGGACTTGTTCTTGGACCCGCTGTTGCAGAAGAAAATTCAGTGTCAACAATCTCATATCTTAAAGGTAGGAGGAGAAACTTCTTTAAAGACTGTTCCACATTTGTGGGAGTACTTGTCTTGTTTTGCTTAGCTGAAAATTATGAGACAACAGGAGCCGCTGAAATGTATGAAAATGGGGTTCTGGCAGTGGTGGATGTGAAGTTTTTGGTGGATGTCCATATCCGAGCAATGGAAGATTCCTCGACGGGTGGTCGGTACTTCTGCTTCGATCAGATAGTGAATAGTGAGGATGAAACTGTTAAACTTGCGAATACATTGAGGCCATTGATATCCATTCCACCGAGGTGCGAAGGGCAAGGGAGGGAAGGTTTTGCTGAGAGGCTGAGAAGCAGGAGATTGCACAAACTGATAGAGGGCGCAGCTTCTTGATAACTTTTGAATGTAAAAACCTTTTGGTTTTCTTGTATGCATCTCTCGCTTCCCTCTTTCTCTCTTCT

Coding sequence (CDS)

ATGGTGGATTTGGAGGTGAGAGGGACAATTAATGTTGTGGAGGCCTGTGCTCGGACAGATACTGTTGAGAAGATAGTCTTCACCTCCTCCTTGACTGCTGCCGTATGGAGAGAGAATATTCTTTCTGAGAAGGATGTGGATGAAAGCTCTGTTACGTTCTCCCAGTTGTGGTATCCTCTAGCAAAGACACTCTCTGAACAAGCAGCCTGGGCTCTAGCAATGGACCGTAGGCTGAACATGGTGTCCATTAACGCAGGACTTGTTCTTGGACCCGCTGTTGCAGAAGAAAATTCAGTGTCAACAATCTCATATCTTAAAGGTAGGAGGAGAAACTTCTTTAAAGACTGTTCCACATTTGTGGGAGTACTTGTCTTGTTTTGCTTAGCTGAAAATTATGAGACAACAGGAGCCGCTGAAATGTATGAAAATGGGGTTCTGGCAGTGGTGGATGTGAAGTTTTTGGTGGATGTCCATATCCGAGCAATGGAAGATTCCTCGACGGGTGGTCGGTACTTCTGCTTCGATCAGATAGTGAATAGTGAGGATGAAACTGTTAAACTTGCGAATACATTGAGGCCATTGATATCCATTCCACCGAGGTGCGAAGGGCAAGGGAGGGAAGGTTTTGCTGAGAGGCTGAGAAGCAGGAGATTGCACAAACTGATAGAGGGCGCAGCTTCTTGA

Protein sequence

MVDLEVRGTINVVEACARTDTVEKIVFTSSLTAAVWRENILSEKDVDESSVTFSQLWYPLAKTLSEQAAWALAMDRRLNMVSINAGLVLGPAVAEENSVSTISYLKGRRRNFFKDCSTFVGVLVLFCLAENYETTGAAEMYENGVLAVVDVKFLVDVHIRAMEDSSTGGRYFCFDQIVNSEDETVKLANTLRPLISIPPRCEGQGREGFAERLRSRRLHKLIEGAAS
Homology
BLAST of Carg14063 vs. NCBI nr
Match: KAG7035704.1 (Cinnamoyl-CoA reductase-like SNL6, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 447.2 bits (1149), Expect = 8.4e-122
Identity = 227/227 (100.00%), Postives = 227/227 (100.00%), Query Frame = 0

Query: 1   MVDLEVRGTINVVEACARTDTVEKIVFTSSLTAAVWRENILSEKDVDESSVTFSQLWYPL 60
           MVDLEVRGTINVVEACARTDTVEKIVFTSSLTAAVWRENILSEKDVDESSVTFSQLWYPL
Sbjct: 1   MVDLEVRGTINVVEACARTDTVEKIVFTSSLTAAVWRENILSEKDVDESSVTFSQLWYPL 60

Query: 61  AKTLSEQAAWALAMDRRLNMVSINAGLVLGPAVAEENSVSTISYLKGRRRNFFKDCSTFV 120
           AKTLSEQAAWALAMDRRLNMVSINAGLVLGPAVAEENSVSTISYLKGRRRNFFKDCSTFV
Sbjct: 61  AKTLSEQAAWALAMDRRLNMVSINAGLVLGPAVAEENSVSTISYLKGRRRNFFKDCSTFV 120

Query: 121 GVLVLFCLAENYETTGAAEMYENGVLAVVDVKFLVDVHIRAMEDSSTGGRYFCFDQIVNS 180
           GVLVLFCLAENYETTGAAEMYENGVLAVVDVKFLVDVHIRAMEDSSTGGRYFCFDQIVNS
Sbjct: 121 GVLVLFCLAENYETTGAAEMYENGVLAVVDVKFLVDVHIRAMEDSSTGGRYFCFDQIVNS 180

Query: 181 EDETVKLANTLRPLISIPPRCEGQGREGFAERLRSRRLHKLIEGAAS 228
           EDETVKLANTLRPLISIPPRCEGQGREGFAERLRSRRLHKLIEGAAS
Sbjct: 181 EDETVKLANTLRPLISIPPRCEGQGREGFAERLRSRRLHKLIEGAAS 227

BLAST of Carg14063 vs. NCBI nr
Match: XP_022931197.1 (cinnamoyl-CoA reductase-like SNL6 isoform X1 [Cucurbita moschata])

HSP 1 Score: 350.9 bits (899), Expect = 8.2e-93
Identity = 193/233 (82.83%), Postives = 194/233 (83.26%), Query Frame = 0

Query: 1   MVDLEVRGTINVVEACARTDTVEKIVFTSSLTAAVWRENILSEKDVDE---SSVTF---S 60
           MVDLEVRGTINVVEACARTDTVEKIVFTSSLTAAVWRENILSEKDVDE   S   F    
Sbjct: 95  MVDLEVRGTINVVEACARTDTVEKIVFTSSLTAAVWRENILSEKDVDERCWSDKEFCRKM 154

Query: 61  QLWYPLAKTLSEQAAWALAMDRRLNMVSINAGLVLGPAVAEENSVSTISYLKGRRRNFFK 120
           +LWYPLAKTLSEQAAWALAMDRRLNMVSINAGLVLGPAVAEENSVSTISYLK        
Sbjct: 155 KLWYPLAKTLSEQAAWALAMDRRLNMVSINAGLVLGPAVAEENSVSTISYLK-------- 214

Query: 121 DCSTFVGVLVLFCLAENYETTGAAEMYENGVLAVVDVKFLVDVHIRAMEDSSTGGRYFCF 180
                                GAAEMYENGVLAVVDVKFLVDVHIRAMEDSSTGGRYFCF
Sbjct: 215 ---------------------GAAEMYENGVLAVVDVKFLVDVHIRAMEDSSTGGRYFCF 274

Query: 181 DQIVNSEDETVKLANTLRPLISIPPRCEGQGREGFAERLRSRRLHKLIEGAAS 228
           DQIVNSEDETVKLANTLRPLISIPPRCEGQGREGFAERLRSRRLHKLIEGAAS
Sbjct: 275 DQIVNSEDETVKLANTLRPLISIPPRCEGQGREGFAERLRSRRLHKLIEGAAS 298

BLAST of Carg14063 vs. NCBI nr
Match: KAG6587792.1 (Cinnamoyl-CoA reductase-like SNL6, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 350.5 bits (898), Expect = 1.1e-92
Identity = 191/233 (81.97%), Postives = 195/233 (83.69%), Query Frame = 0

Query: 1   MVDLEVRGTINVVEACARTDTVEKIVFTSSLTAAVWRENILSEKDVDE---SSVTF---S 60
           MVDLEVRGTINVVEACARTDTV+K+VFTSSLTAAVWRENILSEKDVDE   S + F    
Sbjct: 95  MVDLEVRGTINVVEACARTDTVDKMVFTSSLTAAVWRENILSEKDVDERCWSDIEFCRKM 154

Query: 61  QLWYPLAKTLSEQAAWALAMDRRLNMVSINAGLVLGPAVAEENSVSTISYLKGRRRNFFK 120
           +LWYPLAKTLSEQAAWALAMDRRLNMVSINAGLVLGPAVAEENSVSTISYLK        
Sbjct: 155 KLWYPLAKTLSEQAAWALAMDRRLNMVSINAGLVLGPAVAEENSVSTISYLK-------- 214

Query: 121 DCSTFVGVLVLFCLAENYETTGAAEMYENGVLAVVDVKFLVDVHIRAMEDSSTGGRYFCF 180
                                GAAEMYENGVLAVVDVKFLVDVHIRAMEDSSTGGRYFCF
Sbjct: 215 ---------------------GAAEMYENGVLAVVDVKFLVDVHIRAMEDSSTGGRYFCF 274

Query: 181 DQIVNSEDETVKLANTLRPLISIPPRCEGQGREGFAERLRSRRLHKLIEGAAS 228
           DQIVNSEDETVKLANTLRPLISIPPRCEGQGREGFAERLRSRRLHKLIEGAAS
Sbjct: 275 DQIVNSEDETVKLANTLRPLISIPPRCEGQGREGFAERLRSRRLHKLIEGAAS 298

BLAST of Carg14063 vs. NCBI nr
Match: XP_023001220.1 (cinnamoyl-CoA reductase-like SNL6 isoform X1 [Cucurbita maxima])

HSP 1 Score: 349.7 bits (896), Expect = 1.8e-92
Identity = 192/233 (82.40%), Postives = 194/233 (83.26%), Query Frame = 0

Query: 1   MVDLEVRGTINVVEACARTDTVEKIVFTSSLTAAVWRENILSEKDVDE---SSVTF---S 60
           MVDLEVRGTINVVEACARTDTV+KIVFTSSLTAAVWRENILSEKDVDE   S   F    
Sbjct: 95  MVDLEVRGTINVVEACARTDTVDKIVFTSSLTAAVWRENILSEKDVDERCWSDKEFCRKM 154

Query: 61  QLWYPLAKTLSEQAAWALAMDRRLNMVSINAGLVLGPAVAEENSVSTISYLKGRRRNFFK 120
           +LWYPLAKTLSEQAAWALAMDRRLNMVSINAGLVLGPAVAEENSVSTISYLK        
Sbjct: 155 KLWYPLAKTLSEQAAWALAMDRRLNMVSINAGLVLGPAVAEENSVSTISYLK-------- 214

Query: 121 DCSTFVGVLVLFCLAENYETTGAAEMYENGVLAVVDVKFLVDVHIRAMEDSSTGGRYFCF 180
                                GAAEMYENGVLAVVDVKFLVDVHIRAMEDSSTGGRYFCF
Sbjct: 215 ---------------------GAAEMYENGVLAVVDVKFLVDVHIRAMEDSSTGGRYFCF 274

Query: 181 DQIVNSEDETVKLANTLRPLISIPPRCEGQGREGFAERLRSRRLHKLIEGAAS 228
           DQIVNSEDETVKLANTLRPLISIPPRCEGQGREGFAERLRSRRLHKLIEGAAS
Sbjct: 275 DQIVNSEDETVKLANTLRPLISIPPRCEGQGREGFAERLRSRRLHKLIEGAAS 298

BLAST of Carg14063 vs. NCBI nr
Match: XP_023530489.1 (cinnamoyl-CoA reductase-like SNL6 isoform X1 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 345.1 bits (884), Expect = 4.5e-91
Identity = 190/233 (81.55%), Postives = 193/233 (82.83%), Query Frame = 0

Query: 1   MVDLEVRGTINVVEACARTDTVEKIVFTSSLTAAVWRENILSEKDVDE---SSVTF---S 60
           MVDLEVRGTINVVEACARTDTV+KIVFTSSLTAAVWRENILSEKDVDE   S   F    
Sbjct: 95  MVDLEVRGTINVVEACARTDTVDKIVFTSSLTAAVWRENILSEKDVDERCWSDKEFCRKM 154

Query: 61  QLWYPLAKTLSEQAAWALAMDRRLNMVSINAGLVLGPAVAEENSVSTISYLKGRRRNFFK 120
           +LWYPLAKTLSEQAAWALAMDRRLNMVSINAGLVLGPAVAEENSVSTISYLK        
Sbjct: 155 KLWYPLAKTLSEQAAWALAMDRRLNMVSINAGLVLGPAVAEENSVSTISYLK-------- 214

Query: 121 DCSTFVGVLVLFCLAENYETTGAAEMYENGVLAVVDVKFLVDVHIRAMEDSSTGGRYFCF 180
                                GAAEMYENGVLAVVDVKFLVDVHIRAMEDSSTGGRYFCF
Sbjct: 215 ---------------------GAAEMYENGVLAVVDVKFLVDVHIRAMEDSSTGGRYFCF 274

Query: 181 DQIVNSEDETVKLANTLRPLISIPPRCEGQGREGFAERLRSRRLHKLIEGAAS 228
           DQIVNSEDET+KLANTLRPLISIPPR EGQGREGFAERLRSRRLHKLIEGAAS
Sbjct: 275 DQIVNSEDETIKLANTLRPLISIPPRYEGQGREGFAERLRSRRLHKLIEGAAS 298

BLAST of Carg14063 vs. ExPASy Swiss-Prot
Match: Q0JKZ0 (Cinnamoyl-CoA reductase-like SNL6 OS=Oryza sativa subsp. japonica OX=39947 GN=SNL6 PE=3 SV=1)

HSP 1 Score: 115.5 bits (288), Expect = 7.6e-25
Identity = 69/204 (33.82%), Postives = 99/204 (48.53%), Query Frame = 0

Query: 1   MVDLEVRGTINVVEACARTDTVEKIVFTSSLTAAVWRENILSEKDV----------DESS 60
           M  LE +    V+EAC RT++V K VFTSSL A VWR+N   ++            DES 
Sbjct: 155 MASLEAKAAEQVIEACVRTESVRKCVFTSSLLACVWRQNYPHDRRFPTIIDENCWSDESF 214

Query: 61  VTFSQLWYPLAKTLSEQAAWALAMDRRLNMVSINAGLVLGPAVAEENSVSTISYLKGRRR 120
              ++LW+ L KT +E+ AW  A  R L +V++   LV GP     NS ++I+YLKG R 
Sbjct: 215 CRDNKLWFALGKTAAEKTAWRAARGRDLKLVTVCPALVTGPGFRRRNSTASIAYLKGAR- 274

Query: 121 NFFKDCSTFVGVLVLFCLAENYETTGAAEMYENGVLAVVDVKFLVDVHIR---AMEDSST 180
                                        M  +G+LA   V+ + + H+R   AM D++ 
Sbjct: 275 ----------------------------AMLADGLLATASVETVAEAHVRVYEAMGDNTA 329

Query: 181 GGRYFCFDQIVNSEDETVKLANTL 192
           GGRY C+D +V   +E  +L   L
Sbjct: 335 GGRYICYDHVVKRPEEFAELERQL 329

BLAST of Carg14063 vs. ExPASy Swiss-Prot
Match: A0A0B6VQ48 (Phenylacetaldehyde reductase OS=Rosa hybrid cultivar OX=128735 GN=PAR PE=1 SV=1)

HSP 1 Score: 89.0 bits (219), Expect = 7.6e-17
Identity = 58/208 (27.88%), Postives = 104/208 (50.00%), Query Frame = 0

Query: 1   MVDLEVRGTINVVEACARTDTVEKIVFTSSLTAAVWR-ENILSEKDVDESSVT------F 60
           ++D  V+GT+NV+ +C+++ +++++V TSS+ A  +  +    +  VDE+  T       
Sbjct: 97  LLDPAVKGTLNVLNSCSKSPSIKRVVLTSSIAAVAYNGKPRTPDVVVDETWFTDPDVCKE 156

Query: 61  SQLWYPLAKTLSEQAAWALAMDRRLNMVSINAGLVLGPAVAEENSVSTISYLKGRRRNFF 120
           S+LWY L+KTL+E AAW    ++ ++MV+IN  +V+GP +    + S  + L     N  
Sbjct: 157 SKLWYVLSKTLAEDAAWKFVKEKGIDMVTINPAMVIGPLLQPTLNTSAAAIL-----NII 216

Query: 121 KDCSTFVGVLVLFCLAENYETTGAAEMYENGVLAVVDVKFLVDVHIRAMEDSSTGGRYFC 180
           K                       A  Y N     ++VK + + H++A E  S  GRY C
Sbjct: 217 K----------------------GARTYPNASFGWINVKDVANAHVQAFEIPSASGRY-C 276

Query: 181 FDQIVNSEDETVKLANTLRPLISIPPRC 202
             + V    E +++ + L P + +P +C
Sbjct: 277 LVERVAHFTEVLQIIHELYPDLQLPEKC 276

BLAST of Carg14063 vs. ExPASy Swiss-Prot
Match: Q9CA28 (Tetraketide alpha-pyrone reductase 2 OS=Arabidopsis thaliana OX=3702 GN=TKPR2 PE=1 SV=1)

HSP 1 Score: 82.8 bits (203), Expect = 5.5e-15
Identity = 58/228 (25.44%), Postives = 105/228 (46.05%), Query Frame = 0

Query: 1   MVDLEVRGTINVVEACARTD-TVEKIVFTSSLTAAVWRENILSEKDVDESSVTFSQ---- 60
           +VD  ++GT NV+ +CA++  T+++IV TSS ++  +R +      ++ES  +  +    
Sbjct: 96  LVDPIIKGTTNVMSSCAKSKATLKRIVLTSSCSSIRYRFDATEASPLNESHWSDPEYCKR 155

Query: 61  --LWYPLAKTLSEQAAWALAMDRRLNMVSINAGLVLGPAVAEENSVSTISYLKGRRRNFF 120
             LWY  AKTL E+ AW +A ++ L++V +N   V+GP +                    
Sbjct: 156 FNLWYGYAKTLGEREAWRIAEEKGLDLVVVNPSFVVGPLLGP------------------ 215

Query: 121 KDCSTFVGVLVLFCLAENYETTGAAEMYENGVLAVVDVKFLVDVHIRAMEDSSTGGRYFC 180
           K  ST + +L +          G A  Y N  +  V +  +V  H+ AME+    GR  C
Sbjct: 216 KPTSTLLMILAI--------AKGLAGEYPNFTVGFVHIDDVVAAHVLAMEEPKASGRIIC 275

Query: 181 FDQIVNSEDETVKLANTLRPLISIPPRCEGQGREGFAERLRSRRLHKL 222
              + +   E ++L     P      +C  +  +     + +R++H+L
Sbjct: 276 SSSVAH-WSEIIELMRNKYPNYPFENKCSNKEGDNSPHSMDTRKIHEL 296

BLAST of Carg14063 vs. ExPASy Swiss-Prot
Match: Q500U8 (Tetraketide alpha-pyrone reductase 1 OS=Arabidopsis thaliana OX=3702 GN=TKPR1 PE=1 SV=1)

HSP 1 Score: 77.4 bits (189), Expect = 2.3e-13
Identity = 55/208 (26.44%), Postives = 97/208 (46.63%), Query Frame = 0

Query: 6   VRGTINVVEACARTDTVEKIVFTSSLTAAVWRENILSEKDVDESSVTF------SQLWYP 65
           + GT+NV+ +C +  +++++V TSS +    R++   +  +DES  T        Q+WY 
Sbjct: 104 IEGTLNVLRSCRKNPSLKRVVLTSSSSTVRIRDDFDPKIPLDESIWTSVELCKRFQVWYA 163

Query: 66  LAKTLSEQAAWALAMDRRLNMVSINAGLVLGPAVAEENSVSTISYLKGRRRNFFKDCSTF 125
           L+KTL+EQAAW  + +  +++V++    ++GP++  +                   CST 
Sbjct: 164 LSKTLAEQAAWKFSEENGIDLVTVLPSFLVGPSLPPDL------------------CSTA 223

Query: 126 VGVLVLFCLAENYETTGAAEMYE-NGVLAVVDVKFLVDVHIRAMEDSSTGGRYFCFDQIV 185
             VL L          G  E ++ +G +  V +  +   HI   E  +  GRY C   ++
Sbjct: 224 SDVLGLL--------KGETEKFQWHGQMGYVHIDDVARTHIVVFEHEAAQGRYICSSNVI 283

Query: 186 NSEDETVKLANTLRPLISIPPRCEGQGR 207
           + E E V   +   P + IP R E   R
Sbjct: 284 SLE-ELVSFLSARYPSLPIPKRFEKLNR 284

BLAST of Carg14063 vs. ExPASy Swiss-Prot
Match: Q5XLY0 (Putative anthocyanidin reductase OS=Ginkgo biloba OX=3311 PE=2 SV=1)

HSP 1 Score: 63.2 bits (152), Expect = 4.5e-09
Identity = 50/178 (28.09%), Postives = 82/178 (46.07%), Query Frame = 0

Query: 6   VRGTINVVEACARTDTVEKIVFTSSLTAAVWRENILSEKDVDESSVTFSQL--------W 65
           + GT+NV+++C + D+++++V TSS        +    + +DES  T            W
Sbjct: 111 IEGTLNVLKSCTKVDSIKRVVVTSSAATVSINNSSEQNQYIDESCWTDVNFLTSQKPPGW 170

Query: 66  -YPLAKTLSEQAAWALAMDRRLNMVSINAGLVLGPAVAEENSVSTISYLKGRRRNFFKDC 125
            YP++KTL+EQAA   A +  L++V++   LV+GPAV      S    L     + FK  
Sbjct: 171 AYPVSKTLAEQAALKYAEEHSLDVVTVIPVLVVGPAVTPTVPSSVELALSLITGDEFK-- 230

Query: 126 STFVGVLVLFCLAENYETTGAAEMYENGVLAVVDVKFLVDVHIRAMEDSSTGGRYFCF 175
              +G L                 + +G +++V +  +    I  ME  S  GRY CF
Sbjct: 231 ---MGAL-------------KGMQFVSGSISLVHIDDVCSAQIFLMEKPSAQGRYICF 270

BLAST of Carg14063 vs. ExPASy TrEMBL
Match: A0A6J1ESY8 (cinnamoyl-CoA reductase-like SNL6 isoform X1 OS=Cucurbita moschata OX=3662 GN=LOC111437449 PE=4 SV=1)

HSP 1 Score: 350.9 bits (899), Expect = 4.0e-93
Identity = 193/233 (82.83%), Postives = 194/233 (83.26%), Query Frame = 0

Query: 1   MVDLEVRGTINVVEACARTDTVEKIVFTSSLTAAVWRENILSEKDVDE---SSVTF---S 60
           MVDLEVRGTINVVEACARTDTVEKIVFTSSLTAAVWRENILSEKDVDE   S   F    
Sbjct: 95  MVDLEVRGTINVVEACARTDTVEKIVFTSSLTAAVWRENILSEKDVDERCWSDKEFCRKM 154

Query: 61  QLWYPLAKTLSEQAAWALAMDRRLNMVSINAGLVLGPAVAEENSVSTISYLKGRRRNFFK 120
           +LWYPLAKTLSEQAAWALAMDRRLNMVSINAGLVLGPAVAEENSVSTISYLK        
Sbjct: 155 KLWYPLAKTLSEQAAWALAMDRRLNMVSINAGLVLGPAVAEENSVSTISYLK-------- 214

Query: 121 DCSTFVGVLVLFCLAENYETTGAAEMYENGVLAVVDVKFLVDVHIRAMEDSSTGGRYFCF 180
                                GAAEMYENGVLAVVDVKFLVDVHIRAMEDSSTGGRYFCF
Sbjct: 215 ---------------------GAAEMYENGVLAVVDVKFLVDVHIRAMEDSSTGGRYFCF 274

Query: 181 DQIVNSEDETVKLANTLRPLISIPPRCEGQGREGFAERLRSRRLHKLIEGAAS 228
           DQIVNSEDETVKLANTLRPLISIPPRCEGQGREGFAERLRSRRLHKLIEGAAS
Sbjct: 275 DQIVNSEDETVKLANTLRPLISIPPRCEGQGREGFAERLRSRRLHKLIEGAAS 298

BLAST of Carg14063 vs. ExPASy TrEMBL
Match: A0A6J1KKL4 (cinnamoyl-CoA reductase-like SNL6 isoform X1 OS=Cucurbita maxima OX=3661 GN=LOC111495416 PE=4 SV=1)

HSP 1 Score: 349.7 bits (896), Expect = 8.9e-93
Identity = 192/233 (82.40%), Postives = 194/233 (83.26%), Query Frame = 0

Query: 1   MVDLEVRGTINVVEACARTDTVEKIVFTSSLTAAVWRENILSEKDVDE---SSVTF---S 60
           MVDLEVRGTINVVEACARTDTV+KIVFTSSLTAAVWRENILSEKDVDE   S   F    
Sbjct: 95  MVDLEVRGTINVVEACARTDTVDKIVFTSSLTAAVWRENILSEKDVDERCWSDKEFCRKM 154

Query: 61  QLWYPLAKTLSEQAAWALAMDRRLNMVSINAGLVLGPAVAEENSVSTISYLKGRRRNFFK 120
           +LWYPLAKTLSEQAAWALAMDRRLNMVSINAGLVLGPAVAEENSVSTISYLK        
Sbjct: 155 KLWYPLAKTLSEQAAWALAMDRRLNMVSINAGLVLGPAVAEENSVSTISYLK-------- 214

Query: 121 DCSTFVGVLVLFCLAENYETTGAAEMYENGVLAVVDVKFLVDVHIRAMEDSSTGGRYFCF 180
                                GAAEMYENGVLAVVDVKFLVDVHIRAMEDSSTGGRYFCF
Sbjct: 215 ---------------------GAAEMYENGVLAVVDVKFLVDVHIRAMEDSSTGGRYFCF 274

Query: 181 DQIVNSEDETVKLANTLRPLISIPPRCEGQGREGFAERLRSRRLHKLIEGAAS 228
           DQIVNSEDETVKLANTLRPLISIPPRCEGQGREGFAERLRSRRLHKLIEGAAS
Sbjct: 275 DQIVNSEDETVKLANTLRPLISIPPRCEGQGREGFAERLRSRRLHKLIEGAAS 298

BLAST of Carg14063 vs. ExPASy TrEMBL
Match: A0A6J1ET01 (cinnamoyl-CoA reductase-like SNL6 isoform X2 OS=Cucurbita moschata OX=3662 GN=LOC111437449 PE=4 SV=1)

HSP 1 Score: 322.8 bits (826), Expect = 1.2e-84
Identity = 181/233 (77.68%), Postives = 182/233 (78.11%), Query Frame = 0

Query: 1   MVDLEVRGTINVVEACARTDTVEKIVFTSSLTAAVWRENILSEKDVDE---SSVTF---S 60
           MVDLEVRGTINVVEACARTDTVEKIVFTSSLTAAVWRENILSEKDVDE   S   F    
Sbjct: 95  MVDLEVRGTINVVEACARTDTVEKIVFTSSLTAAVWRENILSEKDVDERCWSDKEFCRKM 154

Query: 61  QLWYPLAKTLSEQAAWALAMDRRLNMVSINAGLVLGPAVAEENSVSTISYLKGRRRNFFK 120
           +LWYPLAKTLSEQAAWALAMDRRLNMVSINAGLVLGPAVAEENSVSTISYLK        
Sbjct: 155 KLWYPLAKTLSEQAAWALAMDRRLNMVSINAGLVLGPAVAEENSVSTISYLK-------- 214

Query: 121 DCSTFVGVLVLFCLAENYETTGAAEMYENGVLAVVDVKFLVDVHIRAMEDSSTGGRYFCF 180
                                            VVDVKFLVDVHIRAMEDSSTGGRYFCF
Sbjct: 215 ---------------------------------VVDVKFLVDVHIRAMEDSSTGGRYFCF 274

Query: 181 DQIVNSEDETVKLANTLRPLISIPPRCEGQGREGFAERLRSRRLHKLIEGAAS 228
           DQIVNSEDETVKLANTLRPLISIPPRCEGQGREGFAERLRSRRLHKLIEGAAS
Sbjct: 275 DQIVNSEDETVKLANTLRPLISIPPRCEGQGREGFAERLRSRRLHKLIEGAAS 286

BLAST of Carg14063 vs. ExPASy TrEMBL
Match: A0A6J1KKM0 (cinnamoyl-CoA reductase-like SNL6 isoform X2 OS=Cucurbita maxima OX=3661 GN=LOC111495416 PE=4 SV=1)

HSP 1 Score: 321.6 bits (823), Expect = 2.6e-84
Identity = 180/233 (77.25%), Postives = 182/233 (78.11%), Query Frame = 0

Query: 1   MVDLEVRGTINVVEACARTDTVEKIVFTSSLTAAVWRENILSEKDVDE---SSVTF---S 60
           MVDLEVRGTINVVEACARTDTV+KIVFTSSLTAAVWRENILSEKDVDE   S   F    
Sbjct: 95  MVDLEVRGTINVVEACARTDTVDKIVFTSSLTAAVWRENILSEKDVDERCWSDKEFCRKM 154

Query: 61  QLWYPLAKTLSEQAAWALAMDRRLNMVSINAGLVLGPAVAEENSVSTISYLKGRRRNFFK 120
           +LWYPLAKTLSEQAAWALAMDRRLNMVSINAGLVLGPAVAEENSVSTISYLK        
Sbjct: 155 KLWYPLAKTLSEQAAWALAMDRRLNMVSINAGLVLGPAVAEENSVSTISYLK-------- 214

Query: 121 DCSTFVGVLVLFCLAENYETTGAAEMYENGVLAVVDVKFLVDVHIRAMEDSSTGGRYFCF 180
                                            VVDVKFLVDVHIRAMEDSSTGGRYFCF
Sbjct: 215 ---------------------------------VVDVKFLVDVHIRAMEDSSTGGRYFCF 274

Query: 181 DQIVNSEDETVKLANTLRPLISIPPRCEGQGREGFAERLRSRRLHKLIEGAAS 228
           DQIVNSEDETVKLANTLRPLISIPPRCEGQGREGFAERLRSRRLHKLIEGAAS
Sbjct: 275 DQIVNSEDETVKLANTLRPLISIPPRCEGQGREGFAERLRSRRLHKLIEGAAS 286

BLAST of Carg14063 vs. ExPASy TrEMBL
Match: A0A0A0LW90 (Cinnamoyl-CoA reductase 1 OS=Cucumis sativus OX=3659 GN=CCR1 PE=2 SV=1)

HSP 1 Score: 319.3 bits (817), Expect = 1.3e-83
Identity = 178/232 (76.72%), Postives = 185/232 (79.74%), Query Frame = 0

Query: 1   MVDLEVRGTINVVEACARTDTVEKIVFTSSLTAAVWRENILSEKDVDE---SSVTF---S 60
           MVDLEVRGTINVVEACA+TD+VEKIVFTSSLTAAVWRENILSEKDVDE   S   F    
Sbjct: 95  MVDLEVRGTINVVEACAQTDSVEKIVFTSSLTAAVWRENILSEKDVDERCWSDKEFCRKM 154

Query: 61  QLWYPLAKTLSEQAAWALAMDRRLNMVSINAGLVLGPAVAEENSVSTISYLKGRRRNFFK 120
           +LWYPLAKTLSEQAAWALAMDRRL MVSINAGLVLGPAVAEENS STISYLK        
Sbjct: 155 KLWYPLAKTLSEQAAWALAMDRRLKMVSINAGLVLGPAVAEENSGSTISYLK-------- 214

Query: 121 DCSTFVGVLVLFCLAENYETTGAAEMYENGVLAVVDVKFLVDVHIRAMEDSSTGGRYFCF 180
                                GAA+MYENGVLAVVDVKFLVDVHIRAMEDSSTGGRYFCF
Sbjct: 215 ---------------------GAAQMYENGVLAVVDVKFLVDVHIRAMEDSSTGGRYFCF 274

Query: 181 DQIVNSEDETVKLANTLRPLISIPPRCEGQGREGFAERLRSRRLHKLIEGAA 227
           DQIVNSE+ETVKLAN LRPLISIP R E QGRE FAERLR+R+LHKLIEG A
Sbjct: 275 DQIVNSEEETVKLANILRPLISIPQRYEVQGREAFAERLRTRKLHKLIEGTA 297

BLAST of Carg14063 vs. TAIR 10
Match: AT4G30470.1 (NAD(P)-binding Rossmann-fold superfamily protein )

HSP 1 Score: 241.1 bits (614), Expect = 8.6e-64
Identity = 133/234 (56.84%), Postives = 164/234 (70.09%), Query Frame = 0

Query: 2   VDLEVRGTINVVEACARTDTVEKIVFTSSLTAAVWRENILSEKDVDE---SSVTF---SQ 61
           VDLEVRG INVVEAC RT+++EKIVF+SSLTA++WR+NI ++KDVDE   S   F    +
Sbjct: 98  VDLEVRGAINVVEACGRTESIEKIVFSSSLTASIWRDNIGTQKDVDEKCWSDQDFCRSKK 157

Query: 62  LWYPLAKTLSEQAAWALAMDRRLNMVSINAGLVLGPAVAEENSVSTISYLKGRRRNFFKD 121
           LW+ LAK LSE+AAWALAMDRRLNMVSIN GLV+GP+VA+ N+  T+SYLK         
Sbjct: 158 LWHALAKMLSEKAAWALAMDRRLNMVSINPGLVVGPSVAQHNARPTMSYLK--------- 217

Query: 122 CSTFVGVLVLFCLAENYETTGAAEMYENGVLAVVDVKFLVDVHIRAMEDSSTGGRYFCFD 181
                               GAA+MYENGVLA VDVKFL DVHIRA ED S  GRYFCF+
Sbjct: 218 --------------------GAAQMYENGVLAYVDVKFLADVHIRAFEDVSACGRYFCFN 277

Query: 182 QIVNSEDETVKLANTLRPLISIPPRCEGQ--GREGFAERLRSRRLHKLIEGAAS 228
           QIVN+E+E +KL  +L PLI +PPR E +  G E + ERLR+ +L KL+E  ++
Sbjct: 278 QIVNTEEEALKLVESLSPLIPMPPRYENEMHGSEVYEERLRNNKLSKLVEAGSA 302

BLAST of Carg14063 vs. TAIR 10
Match: AT2G23910.1 (NAD(P)-binding Rossmann-fold superfamily protein )

HSP 1 Score: 240.7 bits (613), Expect = 1.1e-63
Identity = 131/230 (56.96%), Postives = 165/230 (71.74%), Query Frame = 0

Query: 2   VDLEVRGTINVVEACARTDTVEKIVFTSSLTAAVWRENILSEKDVDE---SSVTF---SQ 61
           VDLEVRG INVVEACART+++EKIVF+SSLTAA+WR+NI ++KDVDE   S + F    +
Sbjct: 99  VDLEVRGAINVVEACARTESIEKIVFSSSLTAAIWRDNIGTQKDVDEKCWSDLDFCLKKK 158

Query: 62  LWYPLAKTLSEQAAWALAMDRRLNMVSINAGLVLGPAVAEENSVSTISYLKGRRRNFFKD 121
           LW+ LAKT SE+AAWALAMDR +NMVS+N GL++GP+VA+ N   T+SYLK         
Sbjct: 159 LWHALAKTQSEKAAWALAMDRMVNMVSVNPGLIVGPSVAQHNPRPTMSYLK--------- 218

Query: 122 CSTFVGVLVLFCLAENYETTGAAEMYENGVLAVVDVKFLVDVHIRAMEDSSTGGRYFCFD 181
                               GAA+MYENGVLA VDV+F+ DVHIRA ED+S  GRYFCF+
Sbjct: 219 --------------------GAAQMYENGVLAYVDVEFVADVHIRAFEDTSACGRYFCFN 278

Query: 182 QIVNSEDETVKLANTLRPLISIPPRCEG--QGREGFAERLRSRRLHKLIE 224
           QIVN+E+E +KL  TL PLI +PPR E   QG E + ERLR+++L+KL+E
Sbjct: 279 QIVNTEEEALKLVQTLSPLIPMPPRHEKEMQGSEVYEERLRNKKLNKLVE 299

BLAST of Carg14063 vs. TAIR 10
Match: AT5G14700.1 (NAD(P)-binding Rossmann-fold superfamily protein )

HSP 1 Score: 108.2 bits (269), Expect = 8.7e-24
Identity = 71/203 (34.98%), Postives = 98/203 (48.28%), Query Frame = 0

Query: 1   MVDLEVRGTINVVEACARTDTVEKIVFTSSLTAAVWREN---------ILSEKDVDESSV 60
           M +LE + + +V+EAC RT +V K VFTSSL A  W++N         I  E   DE   
Sbjct: 150 MAELEAKVSESVIEACTRTASVRKCVFTSSLLACAWQKNPCNSLDHSVINEESWSDEQLC 209

Query: 61  TFSQLWYPLAKTLSEQAAWALAMDRRLNMVSINAGLVLGPAVAEENSVSTISYLKGRRRN 120
             ++LWY L K  +E+AAW +A  + L + +I   L+ GP     NS ST++YLK     
Sbjct: 210 IDNKLWYALGKLKAEKAAWRIADSKGLKLATICPALITGPDFFNRNSTSTLAYLK----- 269

Query: 121 FFKDCSTFVGVLVLFCLAENYETTGAAEMYENGVLAVVDVKFLVDVHI---RAMEDSSTG 180
                                   GA EMY NG+LA +DV  L   H+     + + +  
Sbjct: 270 ------------------------GAKEMYSNGLLATMDVNRLAKAHVCLWEGLGNKTAF 322

Query: 181 GRYFCFDQIVNSEDETVKLANTL 192
           GRY CFD I+ S D   KLA  +
Sbjct: 330 GRYICFDTIL-SRDGAEKLAKDI 322

BLAST of Carg14063 vs. TAIR 10
Match: AT1G51410.1 (NAD(P)-binding Rossmann-fold superfamily protein )

HSP 1 Score: 96.3 bits (238), Expect = 3.4e-20
Identity = 63/211 (29.86%), Postives = 101/211 (47.87%), Query Frame = 0

Query: 1   MVDLEVRGTINVVEACARTDTVEKIVFTSSLTAAVWR------ENILSEK-DVDESSVTF 60
           ++D  V+GTINV+ +C +T +V+++V TSS+ A  +       E I+ E    D      
Sbjct: 100 LLDPAVKGTINVLSSCLKTSSVKRVVLTSSIAAVAFNGMPRTPETIVDETWFADPDYCRA 159

Query: 61  SQLWYPLAKTLSEQAAWALAMDRRLNMVSINAGLVLGPAV---AEENSVSTISYLKGRRR 120
           S+LWY L+KTL+E AAW  A +  L +VSIN  +V+GP +      ++ + +S +KG   
Sbjct: 160 SKLWYVLSKTLAENAAWKFAKENNLQLVSINPAMVIGPLLQPTLNTSAAAVLSLIKG--- 219

Query: 121 NFFKDCSTFVGVLVLFCLAENYETTGAAEMYENGVLAVVDVKFLVDVHIRAMEDSSTGGR 180
                                      A+ + N     V+VK + + HI+A E+    GR
Sbjct: 220 ---------------------------AQTFPNATFGWVNVKDVANAHIQAFENPDADGR 279

Query: 181 YFCFDQIVNSEDETVKLANTLRPLISIPPRC 202
           Y C  + V    E V + + L P   +P +C
Sbjct: 280 Y-CLVERVAHYSEVVNILHDLYPDFQLPEKC 279

BLAST of Carg14063 vs. TAIR 10
Match: AT5G19440.1 (NAD(P)-binding Rossmann-fold superfamily protein )

HSP 1 Score: 92.0 bits (227), Expect = 6.4e-19
Identity = 60/212 (28.30%), Postives = 104/212 (49.06%), Query Frame = 0

Query: 1   MVDLEVRGTINVVEACARTDTVEKIVFTSSLTAAVWR-----------ENILSEKDVDES 60
           ++D  V+GT+NV+ +CA+  +V+++V TSS+ A  +            E   S+ ++ E+
Sbjct: 101 LIDPAVKGTLNVLNSCAKASSVKRVVVTSSMAAVGYNGKPRTPDVTVDETWFSDPELCEA 160

Query: 61  SVTFSQLWYPLAKTLSEQAAWALAMDRRLNMVSINAGLVLGPAVAEENSVSTISYLKGRR 120
               S++WY L+KTL+E AAW LA ++ L++V+IN  +V+GP +    + S  + L    
Sbjct: 161 ----SKMWYVLSKTLAEDAAWKLAKEKGLDIVTINPAMVIGPLLQPTLNTSAAAIL---- 220

Query: 121 RNFFKDCSTFVGVLVLFCLAENYETTGAAEMYENGVLAVVDVKFLVDVHIRAMEDSSTGG 180
                                       A+ + N     V+VK + + HI+A E  S  G
Sbjct: 221 -----------------------NLINGAKTFPNLSFGWVNVKDVANAHIQAFEVPSANG 280

Query: 181 RYFCFDQIVNSEDETVKLANTLRPLISIPPRC 202
           RY   +++V+   E V +   L P + +P RC
Sbjct: 281 RYCLVERVVH-HSEIVNILRELYPNLPLPERC 280

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
KAG7035704.18.4e-122100.00Cinnamoyl-CoA reductase-like SNL6, partial [Cucurbita argyrosperma subsp. argyro... [more]
XP_022931197.18.2e-9382.83cinnamoyl-CoA reductase-like SNL6 isoform X1 [Cucurbita moschata][more]
KAG6587792.11.1e-9281.97Cinnamoyl-CoA reductase-like SNL6, partial [Cucurbita argyrosperma subsp. sorori... [more]
XP_023001220.11.8e-9282.40cinnamoyl-CoA reductase-like SNL6 isoform X1 [Cucurbita maxima][more]
XP_023530489.14.5e-9181.55cinnamoyl-CoA reductase-like SNL6 isoform X1 [Cucurbita pepo subsp. pepo][more]
Match NameE-valueIdentityDescription
Q0JKZ07.6e-2533.82Cinnamoyl-CoA reductase-like SNL6 OS=Oryza sativa subsp. japonica OX=39947 GN=SN... [more]
A0A0B6VQ487.6e-1727.88Phenylacetaldehyde reductase OS=Rosa hybrid cultivar OX=128735 GN=PAR PE=1 SV=1[more]
Q9CA285.5e-1525.44Tetraketide alpha-pyrone reductase 2 OS=Arabidopsis thaliana OX=3702 GN=TKPR2 PE... [more]
Q500U82.3e-1326.44Tetraketide alpha-pyrone reductase 1 OS=Arabidopsis thaliana OX=3702 GN=TKPR1 PE... [more]
Q5XLY04.5e-0928.09Putative anthocyanidin reductase OS=Ginkgo biloba OX=3311 PE=2 SV=1[more]
Match NameE-valueIdentityDescription
A0A6J1ESY84.0e-9382.83cinnamoyl-CoA reductase-like SNL6 isoform X1 OS=Cucurbita moschata OX=3662 GN=LO... [more]
A0A6J1KKL48.9e-9382.40cinnamoyl-CoA reductase-like SNL6 isoform X1 OS=Cucurbita maxima OX=3661 GN=LOC1... [more]
A0A6J1ET011.2e-8477.68cinnamoyl-CoA reductase-like SNL6 isoform X2 OS=Cucurbita moschata OX=3662 GN=LO... [more]
A0A6J1KKM02.6e-8477.25cinnamoyl-CoA reductase-like SNL6 isoform X2 OS=Cucurbita maxima OX=3661 GN=LOC1... [more]
A0A0A0LW901.3e-8376.72Cinnamoyl-CoA reductase 1 OS=Cucumis sativus OX=3659 GN=CCR1 PE=2 SV=1[more]
Match NameE-valueIdentityDescription
AT4G30470.18.6e-6456.84NAD(P)-binding Rossmann-fold superfamily protein [more]
AT2G23910.11.1e-6356.96NAD(P)-binding Rossmann-fold superfamily protein [more]
AT5G14700.18.7e-2434.98NAD(P)-binding Rossmann-fold superfamily protein [more]
AT1G51410.13.4e-2029.86NAD(P)-binding Rossmann-fold superfamily protein [more]
AT5G19440.16.4e-1928.30NAD(P)-binding Rossmann-fold superfamily protein [more]
InterPro
Analysis Name: InterPro Annotations of Silver-seed gourd (SMH-JMG-627) v2
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availableGENE3D3.40.50.720coord: 1..222
e-value: 4.1E-34
score: 120.2
NoneNo IPR availablePANTHERPTHR10366NAD DEPENDENT EPIMERASE/DEHYDRATASEcoord: 2..123
coord: 138..225
NoneNo IPR availablePANTHERPTHR10366:SF390CINNAMOYL-COA REDUCTASE-LIKE PROTEINcoord: 2..123
coord: 138..225
IPR001509NAD-dependent epimerase/dehydratasePFAMPF01370Epimerasecoord: 5..97
e-value: 1.4E-5
score: 24.7
IPR036291NAD(P)-binding domain superfamilySUPERFAMILY51735NAD(P)-binding Rossmann-fold domainscoord: 2..195

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Carg14063-RACarg14063-RAmRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0006694 steroid biosynthetic process
molecular_function GO:0003854 3-beta-hydroxy-delta5-steroid dehydrogenase activity
molecular_function GO:0003824 catalytic activity