Carg14039 (gene) Silver-seed gourd (SMH-JMG-627) v2

Overview
NameCarg14039
Typegene
OrganismCucurbita argyrosperma subsp. argyrosperma cv. SMH-JMG-627 (Silver-seed gourd (SMH-JMG-627) v2)
Descriptionacidic endochitinase-like
LocationCarg_Chr02: 6429391 .. 6433123 (-)
RNA-Seq ExpressionCarg14039
SyntenyCarg14039
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideexonCDS
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGCTGCTCTTTTAGTCATCTTCCTCCTTGCAGCTTCCATATTCCGATCTTCCCATGCCGCCGGAATCGCCATCTATTGGGGCCAAAGTGGCAACGAGGGCTCTCTTGCCTCCACTTGTGCCACCGGAAACTACCAATTCGTCAACATCGCATTTCTCTCCTCCTTCGGCAGCGGCCGAGCTCCGGCACTCAACCTTGCTGGCCACTGCAACCCCAACAACGGCGGCTGCACCTTCTTAAGCAGCCAAATCAAAGCCTGCCAACGTCGAGGCATCAAAGTCCTCCTCTCCATCGGCGGCGGAGCCGGAAGCTACTCGCTCTCCTCCGCCAACGATGCAAGCCAAGTCGCAAATTTCATCTGGAACAACTTCCTCGGTGGCCGGTCGAGATCAAGGCCACTCGGCGACGCCGTTTTAGACGGCGTCGATTTCGATATAGAATCTGGCTCTGGGAAATTCTGGGACGTACTTGCTCGACGACTAAAGGGCTTCGGCCGAGTCCTTCTGGCCGCCGCACCGCAATGTCCGATCCCCGACGCTCACTTGGATGCCGCCATTAAAACCGGTCTGTTTAATTTCGTTTGGGTTCAATTCTACAATAACCCGTCATGCATGTTTGCAAATGGCAACGCTAATAATCTCCTGAATTCTTGGAACCGGTGGAAGACGTTTCCGGTTGGGAAGCTGTTCATGGGGCTTCCGGCGGCACCTTCAGCGGCGCCGAGCGGTGGTTTTATTCCGGCGAACGTGCTTATTTCTCGAGTTCTTCCGAGGATTAAAACTTCTCCCAAGTACGGAGGAGTTATGCTGTGGAGCAAAGCATTTGATAAAGGCTACAGTAACGCAATCAAAGGCAAACTTTGAATAATTACTTAATTATTAAGTAAAAATCCATAAATAACTAAAATAATAAATGAAAAATGGGATTTCTTTCCCATTTTTTTTTAATTATTATAGTACAATAATTGTCGCGTATAATATGAACTAAACTATGCAAGATAATACTAAGCTAAGTACTTAATTTCAATTTAATATTTTAAACCTTCTAATTAAAAATGTCCACGTGGCACAGACAACGACAAAACTGCTATTTCAATCTTCATTTTAAAAAAATTTATTTATTTTTAATTAAAATTTGTAAGGATCGAGTTCATATATATATGTATATATTCTTTTTAATATAATTTTTATTAAAATAATATATACAACATAATTTGGTTTTACATCATCAATTTGGATAAGAACTTGGTAATCGACTCTATCTAGAGTATTATATTTCTATTGATATATCTCACAGTTGAAAATGTAAACAAAAAAAAAATTGTTATTTTAATTCTTTTCGGACTATTTTGGTTTATAATTTTTAAATGGGAGGTTTGGGTTAACGTAGTGAAAGGGTTGTTGCATTCTGAATATCATAAGTTAACTCTAAATTGTGTCACAATTGCATTTTAATGGCAGCGAACTTGCGATTGTGCGGCACTCACTTTCCAACGACAAGTGAGCTAAGCCAATTCGTTCTTGCGTCGCCTACGGCCAAGCGACGTATGCTCAAGCCTCTAGCCTCAATTTTAAAAGAAGGTTTTAGAAAACGAGGGTAGAATGAGATTTTTGAAAGAGACGTTATATAAGCAAGCAAGAGAGAAATAACAACGGCTTGCAGTATATAACATTGGATAGAGAGAAGTTGACAACTAGTCGTATCCTCCCAACAGTATATTCTGAGGCATTTTATGTCTTTTCATATTCCAGTGTTTTAGTCTTATATTTGTAGGAAGTATGACCCAGAACCTAATAGCTCCAGCAATATATTATTTATAATAATATAGAAAATTAAAATCAATATTTAATAATATTATTAAAATCATTTAGATAATCAAATTTTATATAAAATTTACGTGAATGATACGACAAATGATGTCGTATATGTCTTTTAATTAATTTTTAAACATTTTATCATAAACTAATTTTAAGCAAACCAAGAAAATTAGAACTGAAAAAATGTTAAAACAAAATTAGCAAAATTACAACCTTTAAAAAAAAAAAATGTTAAAACATTAAAAAAAAAAAAATGAAATAAGAAAATTTGAAATTTCAAATACGTGTAAAAAAAGTTCTAAAAAATGTATAATAAATTTCTTACTTTTTTTTTTAATCTATAAATTTACTGTGCATTTCATTTTTTTTTTAATTGGTCCATTAAATTTAAAATTGATTAATTTTGTTATTTTTATGTATAGTAAGCTCGTAATTTTTTTAAAAAATAATTGAATAATAAATCAAAGATTTATTAGAAACAACATAATAAAAAATCAAAATTTTATTAAAAACAATTTAAAAATTAACTAAATCATATTCAATTGAATAATAAAAAAAATATTAAATATAAACAATAAGATTAAGCCATCTAATGATTGTAATTAAACAGAAAATTAATAAAAAGAACAATTAGCGGAGGAACGTGTCAAATAATTTATATTTAATATTAGTACAAAATGCAGAGGAGAATAAGTCAAAATTATAGGTAAATTTTGTATTAATTTCCAATTCCAACGAGTCACGGCAGGGCAATTTGGTCATAATAATTTTGTTTGCTTACGTCACCACATTCCAGTCTTCTTAGTTGACCCAAAGACTTTGACTATTTTTAATTTCTTTTCTTTTCTTTCTTTTTTTGAGTTTAATCCATAAATAATAATTTTTTAAAAAATAGAATAATGTAAATAATAATAATTTTTATTTTTCCTCTTCAATTAAAACACGGCAATTCATTTAATAATTTGTAATTTATTTATTTATTTATTTTTTGGTTCCTATAAATTCAGTCCATTTTCTCCATTGTTGAATCACTCAAAAAACCTCATTACAAATGGCTGCTCTTTTAGTTATGTTCCTCCTCGCCGCTTCCATTTTCCGATCATCCCACGCCGCCGGAATCGCCATTTATTGGGGCCAAAACGGCAACGAAGGCTCTCTTGCTTCCACTTGCGCCACCGGAAACTACCAATTTGTCAACGTGGCATTTCTCTCCTCCTTTGGCAACGGCCAAGCTCCGGTACTCAATCTCGCCGGCCACTGCAACCCCGACAACAACGGCTGCGCCTTCTTAAGCAGCGAAATTAACGCCTGCCAAAGTCAAGGCGTCAAAGTCCTCCTCTCAATCGGCGGCGGAGCTGGAAGCTACTCGCTCTCCTCCGCCGATGACGCGGCACAAGTAGCGAATTTCATCTGGAATAATTACCTCGGCGGTCAGTCGAGCTCCAGGCCGCTAGGCGACGCCGTTTTAGACGGAGTCGATTTCGATATCGAAGCCGGGTCGGGTCAATTCTGGGACGAATTGGCCCGGAAACTGAAGGGGTTGGGTCAAGTCGTTATCTCCGCCGCGCCGCAATGTCCGATCCCCGACGCGCACTTGGACACTGCAATTAAAACCGGGCTGTTTGATTTCGTCTGGGTTCAATTCTACAACAACCCGCCGTGCATGTTTACTGATGGAAACACTAACAATCTCCTCAATTCTTGGAACCAGTGGTCCACGTTTCCGGCCGGGAAGCTCTTCATGGGGCTCCCGGCGGCGCCTGAGGCTGCGCCGAGCGGCGGATTTATTCCATCGGATGTCCTTATTTCTCAAATTCTTCCGACCATTAAATCAACTTCCAACTATGGAGGAGTTATGTTGTGGAGCAAGTTTTTCGACAATGGCTACAGCGACGCCATTAAAGGCAGCATCTAA

mRNA sequence

ATGGCTGCTCTTTTAGTCATCTTCCTCCTTGCAGCTTCCATATTCCGATCTTCCCATGCCGCCGGAATCGCCATCTATTGGGGCCAAAGTGGCAACGAGGGCTCTCTTGCCTCCACTTGTGCCACCGGAAACTACCAATTCGTCAACATCGCATTTCTCTCCTCCTTCGGCAGCGGCCGAGCTCCGGCACTCAACCTTGCTGGCCACTGCAACCCCAACAACGGCGGCTGCACCTTCTTAAGCAGCCAAATCAAAGCCTGCCAACGTCGAGGCATCAAAGTCCTCCTCTCCATCGGCGGCGGAGCCGGAAGCTACTCGCTCTCCTCCGCCAACGATGCAAGCCAAGTCGCAAATTTCATCTGGAACAACTTCCTCGGTGGCCGGTCGAGATCAAGGCCACTCGGCGACGCCGTTTTAGACGGCGTCGATTTCGATATAGAATCTGGCTCTGGGAAATTCTGGGACGTACTTGCTCGACGACTAAAGGGCTTCGGCCGATCGAGCTCCAGGCCGCTAGGCGACGCCGTTTTAGACGGAGTCGATTTCGATATCGAAGCCGGGTCGGGTCAATTCTGGGACGAATTGGCCCGGAAACTGAAGGGGTTGGGTCAAGTCGTTATCTCCGCCGCGCCGCAATGTCCGATCCCCGACGCGCACTTGGACACTGCAATTAAAACCGGGCTGTTTGATTTCGTCTGGGTTCAATTCTACAACAACCCGCCGTGCATGTTTACTGATGGAAACACTAACAATCTCCTCAATTCTTGGAACCAGTGGTCCACGTTTCCGGCCGGGAAGCTCTTCATGGGGCTCCCGGCGGCGCCTGAGGCTGCGCCGAGCGGCGGATTTATTCCATCGGATGTCCTTATTTCTCAAATTCTTCCGACCATTAAATCAACTTCCAACTATGGAGGAGTTATGTTGTGGAGCAAGTTTTTCGACAATGGCTACAGCGACGCCATTAAAGGCAGCATCTAA

Coding sequence (CDS)

ATGGCTGCTCTTTTAGTCATCTTCCTCCTTGCAGCTTCCATATTCCGATCTTCCCATGCCGCCGGAATCGCCATCTATTGGGGCCAAAGTGGCAACGAGGGCTCTCTTGCCTCCACTTGTGCCACCGGAAACTACCAATTCGTCAACATCGCATTTCTCTCCTCCTTCGGCAGCGGCCGAGCTCCGGCACTCAACCTTGCTGGCCACTGCAACCCCAACAACGGCGGCTGCACCTTCTTAAGCAGCCAAATCAAAGCCTGCCAACGTCGAGGCATCAAAGTCCTCCTCTCCATCGGCGGCGGAGCCGGAAGCTACTCGCTCTCCTCCGCCAACGATGCAAGCCAAGTCGCAAATTTCATCTGGAACAACTTCCTCGGTGGCCGGTCGAGATCAAGGCCACTCGGCGACGCCGTTTTAGACGGCGTCGATTTCGATATAGAATCTGGCTCTGGGAAATTCTGGGACGTACTTGCTCGACGACTAAAGGGCTTCGGCCGATCGAGCTCCAGGCCGCTAGGCGACGCCGTTTTAGACGGAGTCGATTTCGATATCGAAGCCGGGTCGGGTCAATTCTGGGACGAATTGGCCCGGAAACTGAAGGGGTTGGGTCAAGTCGTTATCTCCGCCGCGCCGCAATGTCCGATCCCCGACGCGCACTTGGACACTGCAATTAAAACCGGGCTGTTTGATTTCGTCTGGGTTCAATTCTACAACAACCCGCCGTGCATGTTTACTGATGGAAACACTAACAATCTCCTCAATTCTTGGAACCAGTGGTCCACGTTTCCGGCCGGGAAGCTCTTCATGGGGCTCCCGGCGGCGCCTGAGGCTGCGCCGAGCGGCGGATTTATTCCATCGGATGTCCTTATTTCTCAAATTCTTCCGACCATTAAATCAACTTCCAACTATGGAGGAGTTATGTTGTGGAGCAAGTTTTTCGACAATGGCTACAGCGACGCCATTAAAGGCAGCATCTAA

Protein sequence

MAALLVIFLLAASIFRSSHAAGIAIYWGQSGNEGSLASTCATGNYQFVNIAFLSSFGSGRAPALNLAGHCNPNNGGCTFLSSQIKACQRRGIKVLLSIGGGAGSYSLSSANDASQVANFIWNNFLGGRSRSRPLGDAVLDGVDFDIESGSGKFWDVLARRLKGFGRSSSRPLGDAVLDGVDFDIEAGSGQFWDELARKLKGLGQVVISAAPQCPIPDAHLDTAIKTGLFDFVWVQFYNNPPCMFTDGNTNNLLNSWNQWSTFPAGKLFMGLPAAPEAAPSGGFIPSDVLISQILPTIKSTSNYGGVMLWSKFFDNGYSDAIKGSI
Homology
BLAST of Carg14039 vs. NCBI nr
Match: KAG7035727.1 (hypothetical protein SDJN02_02525, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 662.1 bits (1707), Expect = 2.4e-186
Identity = 325/325 (100.00%), Postives = 325/325 (100.00%), Query Frame = 0

Query: 1   MAALLVIFLLAASIFRSSHAAGIAIYWGQSGNEGSLASTCATGNYQFVNIAFLSSFGSGR 60
           MAALLVIFLLAASIFRSSHAAGIAIYWGQSGNEGSLASTCATGNYQFVNIAFLSSFGSGR
Sbjct: 1   MAALLVIFLLAASIFRSSHAAGIAIYWGQSGNEGSLASTCATGNYQFVNIAFLSSFGSGR 60

Query: 61  APALNLAGHCNPNNGGCTFLSSQIKACQRRGIKVLLSIGGGAGSYSLSSANDASQVANFI 120
           APALNLAGHCNPNNGGCTFLSSQIKACQRRGIKVLLSIGGGAGSYSLSSANDASQVANFI
Sbjct: 61  APALNLAGHCNPNNGGCTFLSSQIKACQRRGIKVLLSIGGGAGSYSLSSANDASQVANFI 120

Query: 121 WNNFLGGRSRSRPLGDAVLDGVDFDIESGSGKFWDVLARRLKGFGRSSSRPLGDAVLDGV 180
           WNNFLGGRSRSRPLGDAVLDGVDFDIESGSGKFWDVLARRLKGFGRSSSRPLGDAVLDGV
Sbjct: 121 WNNFLGGRSRSRPLGDAVLDGVDFDIESGSGKFWDVLARRLKGFGRSSSRPLGDAVLDGV 180

Query: 181 DFDIEAGSGQFWDELARKLKGLGQVVISAAPQCPIPDAHLDTAIKTGLFDFVWVQFYNNP 240
           DFDIEAGSGQFWDELARKLKGLGQVVISAAPQCPIPDAHLDTAIKTGLFDFVWVQFYNNP
Sbjct: 181 DFDIEAGSGQFWDELARKLKGLGQVVISAAPQCPIPDAHLDTAIKTGLFDFVWVQFYNNP 240

Query: 241 PCMFTDGNTNNLLNSWNQWSTFPAGKLFMGLPAAPEAAPSGGFIPSDVLISQILPTIKST 300
           PCMFTDGNTNNLLNSWNQWSTFPAGKLFMGLPAAPEAAPSGGFIPSDVLISQILPTIKST
Sbjct: 241 PCMFTDGNTNNLLNSWNQWSTFPAGKLFMGLPAAPEAAPSGGFIPSDVLISQILPTIKST 300

Query: 301 SNYGGVMLWSKFFDNGYSDAIKGSI 326
           SNYGGVMLWSKFFDNGYSDAIKGSI
Sbjct: 301 SNYGGVMLWSKFFDNGYSDAIKGSI 325

BLAST of Carg14039 vs. NCBI nr
Match: KAG6605761.1 (hypothetical protein SDJN03_03078, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 537.3 bits (1383), Expect = 8.9e-149
Identity = 268/325 (82.46%), Postives = 281/325 (86.46%), Query Frame = 0

Query: 1   MAALLVIFLLAASIFRSSHAAGIAIYWGQSGNEGSLASTCATGNYQFVNIAFLSSFGSGR 60
           MAALLV+FLLAASIFRSSHAAGIAIYWGQ+GNEGSLASTCATGNYQFVN+AFLSSFG+G+
Sbjct: 1   MAALLVMFLLAASIFRSSHAAGIAIYWGQNGNEGSLASTCATGNYQFVNVAFLSSFGNGQ 60

Query: 61  APALNLAGHCNPNNGGCTFLSSQIKACQRRGIKVLLSIGGGAGSYSLSSANDASQVANFI 120
           AP LNLA HCNP+N GC FLSS+I ACQ +G+KVLLSIGGGAGSYSLSSA+DA+QVANFI
Sbjct: 61  APVLNLASHCNPDNNGCAFLSSEINACQSQGVKVLLSIGGGAGSYSLSSADDAAQVANFI 120

Query: 121 WNNFLGGRSRSRPLGDAVLDGVDFDIESGSGKFWDVLARRLKGFGRSSSRPLGDAVLDGV 180
           WNN+LG                                      G+SSSRPLGDAVLDGV
Sbjct: 121 WNNYLG--------------------------------------GQSSSRPLGDAVLDGV 180

Query: 181 DFDIEAGSGQFWDELARKLKGLGQVVISAAPQCPIPDAHLDTAIKTGLFDFVWVQFYNNP 240
           DFDIEAGSGQFWDELARKLKGLGQVVISAAPQCPIPDAHLDTAIKTGLFDFVWVQFYNNP
Sbjct: 181 DFDIEAGSGQFWDELARKLKGLGQVVISAAPQCPIPDAHLDTAIKTGLFDFVWVQFYNNP 240

Query: 241 PCMFTDGNTNNLLNSWNQWSTFPAGKLFMGLPAAPEAAPSGGFIPSDVLISQILPTIKST 300
           PCMFTDGNTNNLLNSWNQWSTFPAGKLFMGLPAAPEAAPSGGFIPSDVLISQILPTIKST
Sbjct: 241 PCMFTDGNTNNLLNSWNQWSTFPAGKLFMGLPAAPEAAPSGGFIPSDVLISQILPTIKST 287

Query: 301 SNYGGVMLWSKFFDNGYSDAIKGSI 326
           SNYGGVMLWSKFFDNGYSDAIKGSI
Sbjct: 301 SNYGGVMLWSKFFDNGYSDAIKGSI 287

BLAST of Carg14039 vs. NCBI nr
Match: XP_022957794.1 (acidic endochitinase-like [Cucurbita moschata])

HSP 1 Score: 536.2 bits (1380), Expect = 2.0e-148
Identity = 266/325 (81.85%), Postives = 282/325 (86.77%), Query Frame = 0

Query: 1   MAALLVIFLLAASIFRSSHAAGIAIYWGQSGNEGSLASTCATGNYQFVNIAFLSSFGSGR 60
           MAALLV+FLLAASIFRSSHAAGIAIYWGQ+GNEGSLASTCATGNYQFVN+AFLSSFG+G+
Sbjct: 1   MAALLVMFLLAASIFRSSHAAGIAIYWGQNGNEGSLASTCATGNYQFVNVAFLSSFGNGQ 60

Query: 61  APALNLAGHCNPNNGGCTFLSSQIKACQRRGIKVLLSIGGGAGSYSLSSANDASQVANFI 120
           +P LNLAGHCNP+N GC FLSS+I ACQ +G+KVLLSIGGGAGSYSLSSA+DA+QVANFI
Sbjct: 61  SPVLNLAGHCNPDNNGCAFLSSEINACQSQGVKVLLSIGGGAGSYSLSSADDAAQVANFI 120

Query: 121 WNNFLGGRSRSRPLGDAVLDGVDFDIESGSGKFWDVLARRLKGFGRSSSRPLGDAVLDGV 180
           WNN+LG                                      G+SSSRPLG+AVLDGV
Sbjct: 121 WNNYLG--------------------------------------GQSSSRPLGNAVLDGV 180

Query: 181 DFDIEAGSGQFWDELARKLKGLGQVVISAAPQCPIPDAHLDTAIKTGLFDFVWVQFYNNP 240
           DFDIEAGSGQFWDELARKLKGLGQV+ISAAPQCPIPDAHLDTAIKTGLFDFVWVQFYNNP
Sbjct: 181 DFDIEAGSGQFWDELARKLKGLGQVIISAAPQCPIPDAHLDTAIKTGLFDFVWVQFYNNP 240

Query: 241 PCMFTDGNTNNLLNSWNQWSTFPAGKLFMGLPAAPEAAPSGGFIPSDVLISQILPTIKST 300
           PCMFTDGNTNNLLNSWNQWSTFPAGKLFMGLPAAPEAAPSGGFIPSDVLISQILPTIKST
Sbjct: 241 PCMFTDGNTNNLLNSWNQWSTFPAGKLFMGLPAAPEAAPSGGFIPSDVLISQILPTIKST 287

Query: 301 SNYGGVMLWSKFFDNGYSDAIKGSI 326
           SNYGGVMLWSKFFDNGYSDAIKGSI
Sbjct: 301 SNYGGVMLWSKFFDNGYSDAIKGSI 287

BLAST of Carg14039 vs. NCBI nr
Match: XP_023522921.1 (acidic endochitinase-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 534.6 bits (1376), Expect = 5.8e-148
Identity = 266/325 (81.85%), Postives = 281/325 (86.46%), Query Frame = 0

Query: 1   MAALLVIFLLAASIFRSSHAAGIAIYWGQSGNEGSLASTCATGNYQFVNIAFLSSFGSGR 60
           MAALLV+FLLAASIFRSSHAAGIAIYWGQ+GNEGSLASTCATGNYQFVN+AFLSSFG+G+
Sbjct: 1   MAALLVMFLLAASIFRSSHAAGIAIYWGQNGNEGSLASTCATGNYQFVNVAFLSSFGNGQ 60

Query: 61  APALNLAGHCNPNNGGCTFLSSQIKACQRRGIKVLLSIGGGAGSYSLSSANDASQVANFI 120
           +P LNLAGHCNP+N GC FLSS+I ACQ +G+KVLLSIGGGAGSYSLSSA+DA+QVANFI
Sbjct: 61  SPVLNLAGHCNPDNNGCAFLSSEINACQSQGVKVLLSIGGGAGSYSLSSADDAAQVANFI 120

Query: 121 WNNFLGGRSRSRPLGDAVLDGVDFDIESGSGKFWDVLARRLKGFGRSSSRPLGDAVLDGV 180
           WNN+LG                                      G+SSSRPLGDAVLDGV
Sbjct: 121 WNNYLG--------------------------------------GQSSSRPLGDAVLDGV 180

Query: 181 DFDIEAGSGQFWDELARKLKGLGQVVISAAPQCPIPDAHLDTAIKTGLFDFVWVQFYNNP 240
           DFDIEAGSGQFWDELARKLKGLGQVVISAAPQCPIPDAHLDTAIKTGLFD VWVQFYNNP
Sbjct: 181 DFDIEAGSGQFWDELARKLKGLGQVVISAAPQCPIPDAHLDTAIKTGLFDLVWVQFYNNP 240

Query: 241 PCMFTDGNTNNLLNSWNQWSTFPAGKLFMGLPAAPEAAPSGGFIPSDVLISQILPTIKST 300
           PCMFTDGNTNNLLNSWNQWSTFPAGKLFMGLPAAPEAAPSGGFIPSDVLISQILPTIKS+
Sbjct: 241 PCMFTDGNTNNLLNSWNQWSTFPAGKLFMGLPAAPEAAPSGGFIPSDVLISQILPTIKSS 287

Query: 301 SNYGGVMLWSKFFDNGYSDAIKGSI 326
           SNYGGVMLWSKFFDNGYSDAIKGSI
Sbjct: 301 SNYGGVMLWSKFFDNGYSDAIKGSI 287

BLAST of Carg14039 vs. NCBI nr
Match: AAD53006.1 (chitinase [Cucurbita moschata])

HSP 1 Score: 516.2 bits (1328), Expect = 2.1e-142
Identity = 258/325 (79.38%), Postives = 274/325 (84.31%), Query Frame = 0

Query: 1   MAALLVIFLLAASIFRSSHAAGIAIYWGQSGNEGSLASTCATGNYQFVNIAFLSSFGSGR 60
           MAALLV+FLLAASIFRSSHAAGIAIYWGQ+GNEGSLAST ATGNYQFVN+AFLSSFG+G+
Sbjct: 1   MAALLVMFLLAASIFRSSHAAGIAIYWGQNGNEGSLASTYATGNYQFVNVAFLSSFGNGQ 60

Query: 61  APALNLAGHCNPNNGGCTFLSSQIKACQRRGIKVLLSIGGGAGSYSLSSANDASQVANFI 120
           +P LNLA HCNP+N GC FLSS+I ACQ +G+KVLLSIGGGAGSYSLSSA+DA+QVANFI
Sbjct: 61  SPVLNLAFHCNPDNNGCAFLSSEINACQSQGVKVLLSIGGGAGSYSLSSADDAAQVANFI 120

Query: 121 WNNFLGGRSRSRPLGDAVLDGVDFDIESGSGKFWDVLARRLKGFGRSSSRPLGDAVLDGV 180
           WNN+LG                                      G+SSSRP  DAVLDG 
Sbjct: 121 WNNYLG--------------------------------------GQSSSRPATDAVLDGA 180

Query: 181 DFDIEAGSGQFWDELARKLKGLGQVVISAAPQCPIPDAHLDTAIKTGLFDFVWVQFYNNP 240
           DFDIEAGSGQFWDEL  KLKGLGQV+ISAAPQCPIPDAHLDTAIKTGLFDFVWVQFYNNP
Sbjct: 181 DFDIEAGSGQFWDELGPKLKGLGQVIISAAPQCPIPDAHLDTAIKTGLFDFVWVQFYNNP 240

Query: 241 PCMFTDGNTNNLLNSWNQWSTFPAGKLFMGLPAAPEAAPSGGFIPSDVLISQILPTIKST 300
           PCMFTDGNTNNLLNSWNQWSTFPAGKL MGLPAAPEAAP+GGFIPSDVLISQILPTIKST
Sbjct: 241 PCMFTDGNTNNLLNSWNQWSTFPAGKLIMGLPAAPEAAPNGGFIPSDVLISQILPTIKST 287

Query: 301 SNYGGVMLWSKFFDNGYSDAIKGSI 326
           SNYGGVMLWSKFFDNGYSDAIKGSI
Sbjct: 301 SNYGGVMLWSKFFDNGYSDAIKGSI 287

BLAST of Carg14039 vs. ExPASy Swiss-Prot
Match: P17541 (Acidic endochitinase OS=Cucumis sativus OX=3659 PE=1 SV=1)

HSP 1 Score: 463.4 bits (1191), Expect = 2.1e-129
Identity = 230/321 (71.65%), Postives = 257/321 (80.06%), Query Frame = 0

Query: 5   LVIFLLAASIFRSSHAAGIAIYWGQSGNEGSLASTCATGNYQFVNIAFLSSFGSGRAPAL 64
           L IF L +SIFRSS AAGIAIYWGQ+GNEGSLASTCATGNY+FVNIAFLSSFGSG+AP L
Sbjct: 10  LSIFFLLSSIFRSSDAAGIAIYWGQNGNEGSLASTCATGNYEFVNIAFLSSFGSGQAPVL 69

Query: 65  NLAGHCNPNNGGCTFLSSQIKACQRRGIKVLLSIGGGAGSYSLSSANDASQVANFIWNNF 124
           NLAGHCNP+N GC FLS +I +C+ + +KVLLSIGGGAGSYSLSSA+DA QVANFIWN++
Sbjct: 70  NLAGHCNPDNNGCAFLSDEINSCKSQNVKVLLSIGGGAGSYSLSSADDAKQVANFIWNSY 129

Query: 125 LGGRSRSRPLGDAVLDGVDFDIESGSGKFWDVLARRLKGFGRSSSRPLGDAVLDGVDFDI 184
           LGG+S SRPLG AVLDGVDFDIESGSG+FWDVLA+ LK F                    
Sbjct: 130 LGGQSDSRPLGAAVLDGVDFDIESGSGQFWDVLAQELKNF-------------------- 189

Query: 185 EAGSGQFWDELARKLKGLGQVVISAAPQCPIPDAHLDTAIKTGLFDFVWVQFYNNPPCMF 244
                             GQV++SAAPQCPIPDAHLD AIKTGLFD VWVQFYNNPPCMF
Sbjct: 190 ------------------GQVILSAAPQCPIPDAHLDAAIKTGLFDSVWVQFYNNPPCMF 249

Query: 245 TDGNTNNLLNSWNQWSTFPAGKLFMGLPAAPEAAPSGGFIPSDVLISQILPTIKSTSNYG 304
            D N +NLL+SWNQW+ FP  KL+MGLPAA EAAPSGGFIP+DVLISQ+LPTIK++SNYG
Sbjct: 250 AD-NADNLLSSWNQWTAFPTSKLYMGLPAAREAAPSGGFIPADVLISQVLPTIKASSNYG 291

Query: 305 GVMLWSKFFDNGYSDAIKGSI 326
           GVMLWSK FDNGYSD+IKGSI
Sbjct: 310 GVMLWSKAFDNGYSDSIKGSI 291

BLAST of Carg14039 vs. ExPASy Swiss-Prot
Match: P29024 (Acidic endochitinase OS=Phaseolus angularis OX=3914 PE=2 SV=1)

HSP 1 Score: 392.5 bits (1007), Expect = 4.6e-108
Identity = 194/323 (60.06%), Postives = 237/323 (73.37%), Query Frame = 0

Query: 5   LVIFLLAASIFRSSHAAGIAIYWGQSGNEGSLASTCATGNYQFVNIAFLSSFGSGRAPAL 64
           L++ LL  S F+ SHA GI++YWGQ+GNEGSLA  C TGNY++VNIAFL +FG G+ P L
Sbjct: 14  LLLPLLFISFFKPSHAGGISVYWGQNGNEGSLADACNTGNYKYVNIAFLFTFGGGQTPQL 73

Query: 65  NLAGHCNPNNGGCTFLSSQIKACQRRGIKVLLSIGGGAGSYSLSSANDASQVANFIWNNF 124
           NLAGHCNP+   C   S QIK CQ + IKVLLS+GG +GSYSL+SA+DA+QVAN+IWNNF
Sbjct: 74  NLAGHCNPSINNCNVFSDQIKECQSKDIKVLLSLGGASGSYSLTSADDATQVANYIWNNF 133

Query: 125 LGGRSRSRPLGDAVLDGVDFDIESGSGKFWDVLARRLKGFGRSSSRPLGDAVLDGVDFDI 184
           LGG+S SRPLGDA+LDGVDFDIESG+G+ WD LAR LKGF                    
Sbjct: 134 LGGQSSSRPLGDAILDGVDFDIESGTGEHWDDLARALKGFN------------------- 193

Query: 185 EAGSGQFWDELARKLKGLGQVVISAAPQCPIPDAHLDTAIKTGLFDFVWVQFYNNPPCMF 244
                              Q++++AAPQCPIPDAHLDTAIKTGLFD VWVQFYNNPPC +
Sbjct: 194 ------------------SQLLLTAAPQCPIPDAHLDTAIKTGLFDIVWVQFYNNPPCQY 253

Query: 245 TDGNTNNLLNSWNQWSTFPAGKLFMGLPAAPEAAPSGGFIPSDVLISQILPTIKSTSNYG 304
           + GNTN+L++SWNQW++  A +LF+G+PA+  AA S GFIP+DVL SQ+LPTIK +S YG
Sbjct: 254 SSGNTNDLISSWNQWTSSQAKQLFLGVPASTAAAGS-GFIPADVLTSQVLPTIKGSSKYG 298

Query: 305 GVMLWSKFFD--NGYSDAIKGSI 326
           GVMLW +F D  +GYS AI GS+
Sbjct: 314 GVMLWDRFNDGQSGYSGAIIGSV 298

BLAST of Carg14039 vs. ExPASy Swiss-Prot
Match: P36908 (Acidic endochitinase OS=Cicer arietinum OX=3827 PE=2 SV=1)

HSP 1 Score: 384.4 bits (986), Expect = 1.3e-105
Identity = 186/321 (57.94%), Postives = 236/321 (73.52%), Query Frame = 0

Query: 9   LLAASIFRSSHAAGIAIYWGQSGNEGSLASTCATGNYQFVNIAFLSSFGSGRAPALNLAG 68
           LL + + +SS+AAGIA+YWGQ+GNEGSL   C T NYQFVNIAFLS+FG+G+ P +NLAG
Sbjct: 12  LLISLLIKSSNAAGIAVYWGQNGNEGSLQDACNTNNYQFVNIAFLSTFGNGQNPQINLAG 71

Query: 69  HCNPNNGGCTFLSSQIKACQRRGIKVLLSIGGGAGSYSLSSANDASQVANFIWNNFLGGR 128
           HC+P+  GCT  S +I+ACQ +GIKVLLS+GGGAGSYSL+SA +A+ +AN++WNNFLGG 
Sbjct: 72  HCDPSTNGCTKFSPEIQACQAKGIKVLLSLGGGAGSYSLNSAEEATTLANYLWNNFLGGT 131

Query: 129 SRSRPLGDAVLDGVDFDIESGSGKFWDVLARRLKGFGRSSSRPLGDAVLDGVDFDIEAGS 188
           S SRPLGDAVLDG+DFDIESG                                       
Sbjct: 132 STSRPLGDAVLDGIDFDIESG--------------------------------------- 191

Query: 189 GQFWDELARKLKGLGQ--VVISAAPQCPIPDAHLDTAIKTGLFDFVWVQFYNNPPCMFTD 248
           GQ +DELA+ L G  Q  V +SAAPQCP PDAHLD+AI+TGLFD+VWVQFYNNP C +++
Sbjct: 192 GQHYDELAKALNGFSQQKVYLSAAPQCPYPDAHLDSAIQTGLFDYVWVQFYNNPQCQYSN 251

Query: 249 GNTNNLLNSWNQWSTFPAGKLFMGLPAAPEAAPSGGFIPSDVLISQILPTIKSTSNYGGV 308
           GN NNL+N+WNQW++  A ++F+G+PA+  AAPSGG IP+DVL SQ+LP IK++  YGGV
Sbjct: 252 GNINNLVNAWNQWTSSQAKQVFLGVPASDAAAPSGGLIPADVLTSQVLPAIKTSPKYGGV 293

Query: 309 MLWSKFFD--NGYSDAIKGSI 326
           M+W +F D  +GYS+AIKGS+
Sbjct: 312 MIWDRFNDAQSGYSNAIKGSV 293

BLAST of Carg14039 vs. ExPASy Swiss-Prot
Match: P29060 (Acidic endochitinase OS=Nicotiana tabacum OX=4097 PE=1 SV=1)

HSP 1 Score: 368.2 bits (944), Expect = 9.4e-101
Identity = 182/321 (56.70%), Postives = 223/321 (69.47%), Query Frame = 0

Query: 5   LVIFLLAASIFRSSHAAGIAIYWGQSGNEGSLASTCATGNYQFVNIAFLSSFGSGRAPAL 64
           LV+FL A  +     A  I IYWGQ+GNEGSLA TCAT NY  VNIAFL  FG+G+ P L
Sbjct: 11  LVLFLRALKL----EAGDIVIYWGQNGNEGSLADTCATNNYAIVNIAFLVVFGNGQNPVL 70

Query: 65  NLAGHCNPNNGGCTFLSSQIKACQRRGIKVLLSIGGGAGSYSLSSANDASQVANFIWNNF 124
           NLAGHC+PN G CT LS+ I+ACQ +GIKV+LS+GGGAGSY LSSA+DA  VAN++WNN+
Sbjct: 71  NLAGHCDPNAGACTGLSNDIRACQNQGIKVMLSLGGGAGSYFLSSADDARNVANYLWNNY 130

Query: 125 LGGRSRSRPLGDAVLDGVDFDIESGSGKFWDVLARRLKGFGRSSSRPLGDAVLDGVDFDI 184
           LGG+S +RPLGDAVLDG+DFDIE G+ + WD LA+ L  F +                  
Sbjct: 131 LGGQSNTRPLGDAVLDGIDFDIEGGTTQHWDELAKTLSQFSQQR---------------- 190

Query: 185 EAGSGQFWDELARKLKGLGQVVISAAPQCPIPDAHLDTAIKTGLFDFVWVQFYNNPPCMF 244
                              +V ++AAPQCP PD  L+ A+ TGLFD+VWVQFYNNPPC +
Sbjct: 191 -------------------KVYLTAAPQCPFPDTWLNGALSTGLFDYVWVQFYNNPPCQY 250

Query: 245 TDGNTNNLLNSWNQWSTFPAGKLFMGLPAAPEAAPSGGFIPSDVLISQILPTIKSTSNYG 304
           + G+ +NL N WNQW+   AGK+F+GLPAA  AA S GFIPSDVL+SQ+LP I  +  YG
Sbjct: 251 SGGSADNLKNYWNQWNAIQAGKIFLGLPAAQGAAGS-GFIPSDVLVSQVLPLINGSPKYG 291

Query: 305 GVMLWSKFFDNGYSDAIKGSI 326
           GVMLWSKF+DNGYS AIK ++
Sbjct: 311 GVMLWSKFYDNGYSSAIKANV 291

BLAST of Carg14039 vs. ExPASy Swiss-Prot
Match: P23472 (Hevamine-A OS=Hevea brasiliensis OX=3981 PE=1 SV=2)

HSP 1 Score: 368.2 bits (944), Expect = 9.4e-101
Identity = 181/328 (55.18%), Postives = 231/328 (70.43%), Query Frame = 0

Query: 3   ALLVIFLLAASIFRSSH--AAGIAIYWGQSGNEGSLASTCATGNYQFVNIAFLSSFGSGR 62
           A+L++ L  + I  SSH    GIAIYWGQ+GNEG+L  TC+T  Y +VNIAFL+ FG+G+
Sbjct: 7   AILLLLLAISLIMSSSHVDGGGIAIYWGQNGNEGTLTQTCSTRKYSYVNIAFLNKFGNGQ 66

Query: 63  APALNLAGHCNPNNGGCTFLSSQIKACQRRGIKVLLSIGGGAGSYSLSSANDASQVANFI 122
            P +NLAGHCNP  GGCT +S+ I++CQ +GIKV+LS+GGG GSY+L+S  DA  VA+++
Sbjct: 67  TPQINLAGHCNPAAGGCTIVSNGIRSCQIQGIKVMLSLGGGIGSYTLASQADAKNVADYL 126

Query: 123 WNNFLGGRSRSRPLGDAVLDGVDFDIESGSGKFWDVLARRLKGFGRSSSRPLGDAVLDGV 182
           WNNFLGG+S SRPLGDAVLDG+DFDIE GS  +WD LAR L  + +   +          
Sbjct: 127 WNNFLGGKSSSRPLGDAVLDGIDFDIEHGSTLYWDDLARYLSAYSKQGKK---------- 186

Query: 183 DFDIEAGSGQFWDELARKLKGLGQVVISAAPQCPIPDAHLDTAIKTGLFDFVWVQFYNNP 242
                                   V ++AAPQCP PD +L TA+ TGLFD+VWVQFYNNP
Sbjct: 187 ------------------------VYLTAAPQCPFPDRYLGTALNTGLFDYVWVQFYNNP 246

Query: 243 PCMFTDGNTNNLLNSWNQWST-FPAGKLFMGLPAAPEAAPSGGFIPSDVLISQILPTIKS 302
           PC ++ GN NN++NSWN+W+T   AGK+F+GLPAAPEAA S G++P DVLIS+ILP IK 
Sbjct: 247 PCQYSSGNINNIINSWNRWTTSINAGKIFLGLPAAPEAAGS-GYVPPDVLISRILPEIKK 299

Query: 303 TSNYGGVMLWSKFFD--NGYSDAIKGSI 326
           +  YGGVMLWSKF+D  NGYS +I  S+
Sbjct: 307 SPKYGGVMLWSKFYDDKNGYSSSILDSV 299

BLAST of Carg14039 vs. ExPASy TrEMBL
Match: A0A6J1H309 (acidic endochitinase-like OS=Cucurbita moschata OX=3662 GN=LOC111459229 PE=3 SV=1)

HSP 1 Score: 536.2 bits (1380), Expect = 9.6e-149
Identity = 266/325 (81.85%), Postives = 282/325 (86.77%), Query Frame = 0

Query: 1   MAALLVIFLLAASIFRSSHAAGIAIYWGQSGNEGSLASTCATGNYQFVNIAFLSSFGSGR 60
           MAALLV+FLLAASIFRSSHAAGIAIYWGQ+GNEGSLASTCATGNYQFVN+AFLSSFG+G+
Sbjct: 1   MAALLVMFLLAASIFRSSHAAGIAIYWGQNGNEGSLASTCATGNYQFVNVAFLSSFGNGQ 60

Query: 61  APALNLAGHCNPNNGGCTFLSSQIKACQRRGIKVLLSIGGGAGSYSLSSANDASQVANFI 120
           +P LNLAGHCNP+N GC FLSS+I ACQ +G+KVLLSIGGGAGSYSLSSA+DA+QVANFI
Sbjct: 61  SPVLNLAGHCNPDNNGCAFLSSEINACQSQGVKVLLSIGGGAGSYSLSSADDAAQVANFI 120

Query: 121 WNNFLGGRSRSRPLGDAVLDGVDFDIESGSGKFWDVLARRLKGFGRSSSRPLGDAVLDGV 180
           WNN+LG                                      G+SSSRPLG+AVLDGV
Sbjct: 121 WNNYLG--------------------------------------GQSSSRPLGNAVLDGV 180

Query: 181 DFDIEAGSGQFWDELARKLKGLGQVVISAAPQCPIPDAHLDTAIKTGLFDFVWVQFYNNP 240
           DFDIEAGSGQFWDELARKLKGLGQV+ISAAPQCPIPDAHLDTAIKTGLFDFVWVQFYNNP
Sbjct: 181 DFDIEAGSGQFWDELARKLKGLGQVIISAAPQCPIPDAHLDTAIKTGLFDFVWVQFYNNP 240

Query: 241 PCMFTDGNTNNLLNSWNQWSTFPAGKLFMGLPAAPEAAPSGGFIPSDVLISQILPTIKST 300
           PCMFTDGNTNNLLNSWNQWSTFPAGKLFMGLPAAPEAAPSGGFIPSDVLISQILPTIKST
Sbjct: 241 PCMFTDGNTNNLLNSWNQWSTFPAGKLFMGLPAAPEAAPSGGFIPSDVLISQILPTIKST 287

Query: 301 SNYGGVMLWSKFFDNGYSDAIKGSI 326
           SNYGGVMLWSKFFDNGYSDAIKGSI
Sbjct: 301 SNYGGVMLWSKFFDNGYSDAIKGSI 287

BLAST of Carg14039 vs. ExPASy TrEMBL
Match: Q9SQI0 (Chitinase OS=Cucurbita moschata OX=3662 GN=PLC PE=2 SV=1)

HSP 1 Score: 516.2 bits (1328), Expect = 1.0e-142
Identity = 258/325 (79.38%), Postives = 274/325 (84.31%), Query Frame = 0

Query: 1   MAALLVIFLLAASIFRSSHAAGIAIYWGQSGNEGSLASTCATGNYQFVNIAFLSSFGSGR 60
           MAALLV+FLLAASIFRSSHAAGIAIYWGQ+GNEGSLAST ATGNYQFVN+AFLSSFG+G+
Sbjct: 1   MAALLVMFLLAASIFRSSHAAGIAIYWGQNGNEGSLASTYATGNYQFVNVAFLSSFGNGQ 60

Query: 61  APALNLAGHCNPNNGGCTFLSSQIKACQRRGIKVLLSIGGGAGSYSLSSANDASQVANFI 120
           +P LNLA HCNP+N GC FLSS+I ACQ +G+KVLLSIGGGAGSYSLSSA+DA+QVANFI
Sbjct: 61  SPVLNLAFHCNPDNNGCAFLSSEINACQSQGVKVLLSIGGGAGSYSLSSADDAAQVANFI 120

Query: 121 WNNFLGGRSRSRPLGDAVLDGVDFDIESGSGKFWDVLARRLKGFGRSSSRPLGDAVLDGV 180
           WNN+LG                                      G+SSSRP  DAVLDG 
Sbjct: 121 WNNYLG--------------------------------------GQSSSRPATDAVLDGA 180

Query: 181 DFDIEAGSGQFWDELARKLKGLGQVVISAAPQCPIPDAHLDTAIKTGLFDFVWVQFYNNP 240
           DFDIEAGSGQFWDEL  KLKGLGQV+ISAAPQCPIPDAHLDTAIKTGLFDFVWVQFYNNP
Sbjct: 181 DFDIEAGSGQFWDELGPKLKGLGQVIISAAPQCPIPDAHLDTAIKTGLFDFVWVQFYNNP 240

Query: 241 PCMFTDGNTNNLLNSWNQWSTFPAGKLFMGLPAAPEAAPSGGFIPSDVLISQILPTIKST 300
           PCMFTDGNTNNLLNSWNQWSTFPAGKL MGLPAAPEAAP+GGFIPSDVLISQILPTIKST
Sbjct: 241 PCMFTDGNTNNLLNSWNQWSTFPAGKLIMGLPAAPEAAPNGGFIPSDVLISQILPTIKST 287

Query: 301 SNYGGVMLWSKFFDNGYSDAIKGSI 326
           SNYGGVMLWSKFFDNGYSDAIKGSI
Sbjct: 301 SNYGGVMLWSKFFDNGYSDAIKGSI 287

BLAST of Carg14039 vs. ExPASy TrEMBL
Match: A0A6J1H047 (acidic endochitinase-like OS=Cucurbita moschata OX=3662 GN=LOC111459228 PE=3 SV=1)

HSP 1 Score: 509.6 bits (1311), Expect = 9.6e-141
Identity = 253/325 (77.85%), Postives = 271/325 (83.38%), Query Frame = 0

Query: 1   MAALLVIFLLAASIFRSSHAAGIAIYWGQSGNEGSLASTCATGNYQFVNIAFLSSFGSGR 60
           MAALLVIFLLAASIFRSSHAAGIA+YWGQ+GNEG LASTCATGNYQFVNIAFLSSFGSGR
Sbjct: 1   MAALLVIFLLAASIFRSSHAAGIAVYWGQNGNEGPLASTCATGNYQFVNIAFLSSFGSGR 60

Query: 61  APALNLAGHCNPNNGGCTFLSSQIKACQRRGIKVLLSIGGGAGSYSLSSANDASQVANFI 120
           AP LNLAGHCNPNNGGCTFLSSQIKACQRRGIKVLLSIGGGAGSYSLSSA+DASQVANFI
Sbjct: 61  APVLNLAGHCNPNNGGCTFLSSQIKACQRRGIKVLLSIGGGAGSYSLSSADDASQVANFI 120

Query: 121 WNNFLGGRSRSRPLGDAVLDGVDFDIESGSGKFWDVLARRLKGFGRSSSRPLGDAVLDGV 180
           WNNFLGGRSRSRPLGDAVLDGVDFDIE+GSGKFWDVLARRLKGF R              
Sbjct: 121 WNNFLGGRSRSRPLGDAVLDGVDFDIETGSGKFWDVLARRLKGFSR-------------- 180

Query: 181 DFDIEAGSGQFWDELARKLKGLGQVVISAAPQCPIPDAHLDTAIKTGLFDFVWVQFYNNP 240
                                   V+++AAPQCPIPDAHLD AIKTGLF+FVWVQFYNNP
Sbjct: 181 ------------------------VLLAAAPQCPIPDAHLDAAIKTGLFNFVWVQFYNNP 240

Query: 241 PCMFTDGNTNNLLNSWNQWSTFPAGKLFMGLPAAPEAAPSGGFIPSDVLISQILPTIKST 300
            CMF +GN NNLLNSWN+W TFP GKLFMGLPAAP AAPSGGFIP++VLIS++LP IK++
Sbjct: 241 SCMFANGNANNLLNSWNRWKTFPVGKLFMGLPAAPSAAPSGGFIPANVLISRVLPRIKTS 287

Query: 301 SNYGGVMLWSKFFDNGYSDAIKGSI 326
             YGGVMLWSK FD GYS+AIKG +
Sbjct: 301 PKYGGVMLWSKAFDKGYSNAIKGKL 287

BLAST of Carg14039 vs. ExPASy TrEMBL
Match: A0A6J1K3I7 (acidic endochitinase-like OS=Cucurbita maxima OX=3661 GN=LOC111490828 PE=3 SV=1)

HSP 1 Score: 506.5 bits (1303), Expect = 8.1e-140
Identity = 252/325 (77.54%), Postives = 269/325 (82.77%), Query Frame = 0

Query: 1   MAALLVIFLLAASIFRSSHAAGIAIYWGQSGNEGSLASTCATGNYQFVNIAFLSSFGSGR 60
           MAALLVIFLLAASIFRSSHAAGIAIYWGQ+GNEGSLASTCATGNYQFVNIAFLSSFGSGR
Sbjct: 1   MAALLVIFLLAASIFRSSHAAGIAIYWGQNGNEGSLASTCATGNYQFVNIAFLSSFGSGR 60

Query: 61  APALNLAGHCNPNNGGCTFLSSQIKACQRRGIKVLLSIGGGAGSYSLSSANDASQVANFI 120
           AP LNLAGHCNPNNGGCTFLSSQIKACQRRGIKVLLSIGGGAGSYSLSSA+DASQVANFI
Sbjct: 61  APVLNLAGHCNPNNGGCTFLSSQIKACQRRGIKVLLSIGGGAGSYSLSSADDASQVANFI 120

Query: 121 WNNFLGGRSRSRPLGDAVLDGVDFDIESGSGKFWDVLARRLKGFGRSSSRPLGDAVLDGV 180
           WNNFLGGRSRSRP GDA+LDGVDFDIESGSGKFWDVLARRLKGF R              
Sbjct: 121 WNNFLGGRSRSRPFGDAILDGVDFDIESGSGKFWDVLARRLKGFSR-------------- 180

Query: 181 DFDIEAGSGQFWDELARKLKGLGQVVISAAPQCPIPDAHLDTAIKTGLFDFVWVQFYNNP 240
                                   V+++AAPQCPIPDAHLD AIKT LF+FVWVQFYNNP
Sbjct: 181 ------------------------VLLAAAPQCPIPDAHLDAAIKTSLFNFVWVQFYNNP 240

Query: 241 PCMFTDGNTNNLLNSWNQWSTFPAGKLFMGLPAAPEAAPSGGFIPSDVLISQILPTIKST 300
            CMF +GN NNLLNSWN+W TFP GKLFMGLPAAP AAPSGGFIP++VLIS++LP IK++
Sbjct: 241 SCMFANGNANNLLNSWNRWKTFPVGKLFMGLPAAPSAAPSGGFIPANVLISRVLPRIKTS 287

Query: 301 SNYGGVMLWSKFFDNGYSDAIKGSI 326
             YGGVMLWSK FD GYS+AIK  +
Sbjct: 301 PKYGGVMLWSKAFDKGYSNAIKAKL 287

BLAST of Carg14039 vs. ExPASy TrEMBL
Match: A0A6J1K5Q2 (acidic endochitinase OS=Cucurbita maxima OX=3661 GN=LOC111491397 PE=3 SV=1)

HSP 1 Score: 489.6 bits (1259), Expect = 1.0e-134
Identity = 239/301 (79.40%), Postives = 254/301 (84.39%), Query Frame = 0

Query: 25  IYWGQSGNEGSLASTCATGNYQFVNIAFLSSFGSGRAPALNLAGHCNPNNGGCTFLSSQI 84
           +YWGQ+GNEGSLASTCATGNYQFVN+AFLSSFGSG+AP LNLAGHCNP+N GC FLSS+I
Sbjct: 2   VYWGQNGNEGSLASTCATGNYQFVNVAFLSSFGSGQAPVLNLAGHCNPDNNGCAFLSSEI 61

Query: 85  KACQRRGIKVLLSIGGGAGSYSLSSANDASQVANFIWNNFLGGRSRSRPLGDAVLDGVDF 144
            ACQ +G+KVLLSIGGGAGSYSLSSA+DA+QVANFIWNN+LG                  
Sbjct: 62  NACQSQGVKVLLSIGGGAGSYSLSSADDAAQVANFIWNNYLG------------------ 121

Query: 145 DIESGSGKFWDVLARRLKGFGRSSSRPLGDAVLDGVDFDIEAGSGQFWDELARKLKGLGQ 204
                               G+SSSRPLGDAVLDGVDFDIEAGSGQFWDELA +LKGLGQ
Sbjct: 122 --------------------GQSSSRPLGDAVLDGVDFDIEAGSGQFWDELAGQLKGLGQ 181

Query: 205 VVISAAPQCPIPDAHLDTAIKTGLFDFVWVQFYNNPPCMFTDGNTNNLLNSWNQWSTFPA 264
           VVISAAPQCPIPDAHLDTAIKTGLFD VWVQFYNNPPCMFTDGNT+NLLNSWNQWSTFP 
Sbjct: 182 VVISAAPQCPIPDAHLDTAIKTGLFDLVWVQFYNNPPCMFTDGNTDNLLNSWNQWSTFPV 241

Query: 265 GKLFMGLPAAPEAAPSGGFIPSDVLISQILPTIKSTSNYGGVMLWSKFFDNGYSDAIKGS 324
           GKLFMGLPAAPEAAPSGGFIPSDVLISQILPTIKS+SNYGGVMLWSK FDNGYSDAIKGS
Sbjct: 242 GKLFMGLPAAPEAAPSGGFIPSDVLISQILPTIKSSSNYGGVMLWSKSFDNGYSDAIKGS 264

Query: 325 I 326
           I
Sbjct: 302 I 264

BLAST of Carg14039 vs. TAIR 10
Match: AT5G24090.1 (chitinase A )

HSP 1 Score: 346.7 bits (888), Expect = 2.1e-95
Identity = 171/324 (52.78%), Postives = 219/324 (67.59%), Query Frame = 0

Query: 7   IFLLAASIFRSSHAA--GIAIYWGQSGNEGSLASTCATGNYQFVNIAFLSSFGSGRAPAL 66
           +F ++ S+ + S A+  GIAIYWGQ+GNEG+L++TCATG Y +VN+AFL  FG+G+ P L
Sbjct: 14  LFFISCSLSKPSDASRGGIAIYWGQNGNEGNLSATCATGRYAYVNVAFLVKFGNGQTPEL 73

Query: 67  NLAGHCNPNNGGCTFLSSQIKACQRRGIKVLLSIGGGAGSYSLSSANDASQVANFIWNNF 126
           NLAGHCNP    CT   SQ+K CQ RGIKV+LS+GGG G+YS+ S  DA  +A+++WNNF
Sbjct: 74  NLAGHCNPAANTCTHFGSQVKDCQSRGIKVMLSLGGGIGNYSIGSREDAKVIADYLWNNF 133

Query: 127 LGGRSRSRPLGDAVLDGVDFDIESGSGKFWDVLARRLKGFGRSSSRPLGDAVLDGVDFDI 186
           LGG+S SRPLGDAVLDG+DF+IE GS + WD LAR L  F     +              
Sbjct: 134 LGGKSSSRPLGDAVLDGIDFNIELGSPQHWDDLARTLSKFSHRGRK-------------- 193

Query: 187 EAGSGQFWDELARKLKGLGQVVISAAPQCPIPDAHLDTAIKTGLFDFVWVQFYNNPPCMF 246
                               + ++ APQCP PD  + +A+ T  FD+VW+QFYNNPPC +
Sbjct: 194 --------------------IYLTGAPQCPFPDRLMGSALNTKRFDYVWIQFYNNPPCSY 253

Query: 247 TDGNTNNLLNSWNQWST-FPAGKLFMGLPAAPEAAPSGGFIPSDVLISQILPTIKSTSNY 306
           + GNT NL +SWN+W+T   A K F+GLPAAPEAA S G+IP DVL SQILPT+K +  Y
Sbjct: 254 SSGNTQNLFDSWNKWTTSIAAQKFFLGLPAAPEAAGS-GYIPPDVLTSQILPTLKKSRKY 302

Query: 307 GGVMLWSKFFD--NGYSDAIKGSI 326
           GGVMLWSKF+D  NGYS +I  S+
Sbjct: 314 GGVMLWSKFWDDKNGYSSSILASV 302

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
KAG7035727.12.4e-186100.00hypothetical protein SDJN02_02525, partial [Cucurbita argyrosperma subsp. argyro... [more]
KAG6605761.18.9e-14982.46hypothetical protein SDJN03_03078, partial [Cucurbita argyrosperma subsp. sorori... [more]
XP_022957794.12.0e-14881.85acidic endochitinase-like [Cucurbita moschata][more]
XP_023522921.15.8e-14881.85acidic endochitinase-like [Cucurbita pepo subsp. pepo][more]
AAD53006.12.1e-14279.38chitinase [Cucurbita moschata][more]
Match NameE-valueIdentityDescription
P175412.1e-12971.65Acidic endochitinase OS=Cucumis sativus OX=3659 PE=1 SV=1[more]
P290244.6e-10860.06Acidic endochitinase OS=Phaseolus angularis OX=3914 PE=2 SV=1[more]
P369081.3e-10557.94Acidic endochitinase OS=Cicer arietinum OX=3827 PE=2 SV=1[more]
P290609.4e-10156.70Acidic endochitinase OS=Nicotiana tabacum OX=4097 PE=1 SV=1[more]
P234729.4e-10155.18Hevamine-A OS=Hevea brasiliensis OX=3981 PE=1 SV=2[more]
Match NameE-valueIdentityDescription
A0A6J1H3099.6e-14981.85acidic endochitinase-like OS=Cucurbita moschata OX=3662 GN=LOC111459229 PE=3 SV=... [more]
Q9SQI01.0e-14279.38Chitinase OS=Cucurbita moschata OX=3662 GN=PLC PE=2 SV=1[more]
A0A6J1H0479.6e-14177.85acidic endochitinase-like OS=Cucurbita moschata OX=3662 GN=LOC111459228 PE=3 SV=... [more]
A0A6J1K3I78.1e-14077.54acidic endochitinase-like OS=Cucurbita maxima OX=3661 GN=LOC111490828 PE=3 SV=1[more]
A0A6J1K5Q21.0e-13479.40acidic endochitinase OS=Cucurbita maxima OX=3661 GN=LOC111491397 PE=3 SV=1[more]
Match NameE-valueIdentityDescription
AT5G24090.12.1e-9552.78chitinase A [more]
InterPro
Analysis Name: InterPro Annotations of Silver-seed gourd (SMH-JMG-627) v2
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR001223Glycoside hydrolase family 18, catalytic domainPFAMPF00704Glyco_hydro_18coord: 23..163
e-value: 1.5E-14
score: 54.4
IPR001223Glycoside hydrolase family 18, catalytic domainPFAMPF00704Glyco_hydro_18coord: 172..284
e-value: 9.7E-12
score: 45.2
IPR001223Glycoside hydrolase family 18, catalytic domainPROSITEPS51910GH18_2coord: 21..325
score: 41.718025
NoneNo IPR availableGENE3D3.20.20.80Glycosidasescoord: 22..163
e-value: 7.5E-53
score: 181.5
coord: 164..325
e-value: 2.0E-53
score: 183.4
NoneNo IPR availablePANTHERPTHR45708ENDOCHITINASEcoord: 165..324
coord: 5..164
NoneNo IPR availablePANTHERPTHR45708:SF22ACIDIC ENDOCHITINASEcoord: 165..324
coord: 5..164
NoneNo IPR availableCDDcd02877GH18_hevamine_XipI_class_IIIcoord: 21..323
e-value: 3.4639E-128
score: 365.409
IPR001579Glycosyl hydrolases family 18 (GH18) active sitePROSITEPS01095GH18_1coord: 177..185
IPR017853Glycoside hydrolase superfamilySUPERFAMILY51445(Trans)glycosidasescoord: 19..164
IPR017853Glycoside hydrolase superfamilySUPERFAMILY51445(Trans)glycosidasescoord: 167..316

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Carg14039-RACarg14039-RAmRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0006032 chitin catabolic process
biological_process GO:0000272 polysaccharide catabolic process
biological_process GO:0005975 carbohydrate metabolic process
cellular_component GO:0005615 extracellular space
molecular_function GO:0004568 chitinase activity
molecular_function GO:0004553 hydrolase activity, hydrolyzing O-glycosyl compounds