Carg14021 (gene) Silver-seed gourd (SMH-JMG-627) v2

Overview
NameCarg14021
Typegene
OrganismCucurbita argyrosperma subsp. argyrosperma cv. SMH-JMG-627 (Silver-seed gourd (SMH-JMG-627) v2)
DescriptionSpermidine synthase
LocationCarg_Chr02: 6517585 .. 6522605 (-)
RNA-Seq ExpressionCarg14021
SyntenyCarg14021
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRpolypeptideCDSthree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
AGACCAAGCACGAAGCAAAAGAAGAAGGAAACTGGTTCAAGGCCTAAATCTTCTCCCTGTGAGCCATAGTCGAGATTAGGATGGGCCTTTAAATGGTGCATACGTCATACATGCCCTTGGAATAATTTCGTTCGGCTCGATTTGATTATTTGTGTAGAGGTAACATGAAAGTCATAAATTATTTCTGTCATAAACGTATTTAATTTTTAGGAATAATAATGGAATTAAAATAACAATAGATCATAAAATTACATTTTCCAAATTATTTTTAATTTAATTCCTTAGTTCTTCTTCTATATTAATTATTTAATCGTTCATTTAATTTTTTTTAATTAACATTTAATATTTTTATTATTGGTAGAACTTATTTTTGAAAAAGAAATTTAAATAAGAATAATTTAGATATTATTAATTATTGTTACCTAAAAGTTATGGCTAAATGTTAATGTCCAAATTTTGTGTGACATGAAAGGTCTGGAATGGAGGAGTTTTGTGGCTCGAGAATCTTTGAGATGGGGTCCTGACTTTCAATTTTTTCAACATTGATCGCGTGCTAAATACAGGTTCGTCCTCGTCGGGTCGCGGCACGATTTCTGATCCGCCCACCTAATCGAGATTCATGATCTTTCTTTCAATTTTCTATATATAAATGTAATGCCCCAAATCCCAATTTTCATCATACTTCAACGAGAATCGGAAACCAGAGCATACAGAGAGAGGAGTGAAACGAAGAGATTGAGTGTGTTTGTGTACGAGAGAATTCTTTTTGCGTCTCTTTCTTGCTTGTTTGCGTCTTTTCCAGAAGTTGGAAGTTCCCATTTCCAGAAATTAACGCCTGGTAATCTCTCTGTCGTTAACCCTTTTTGACTCTGCTTTGATTGTTCTTGCTCTGTAATCTATGGCTTCGCTCTGAGTTTGGTTTGATTGTTGCTTAAATGCATTTTTCTTCTGTTTTGATGGATTCTGTTTGTTTCCTCGGATTTTTTTTGTCGTTTGATTACGGATACATATTGTGGGTATGTATCCCTTCTATCTCTCTCTCTCTCTCTGTCTTCTTGTTCTTCCGATTGTTGAGTTTAATATTGCGTTCGTGCTCAAATACTGCTATCATTTTCACTTTCGTCCTCCTTGCCCTTCAATCTCTCGGACTCAAACTGGAAATTTCGGGCTATAGAATGATTTATCCTTAGTCTTACGATTTTCCCGTTCATTTTTTCGTTTTTGAATCATAGAGCCATCTTGTTTGAGAAATTGTTGGTTAATATTGTTCTGTTTTGATCGAATCGTTGGAGATATATGTATCGCTTTAAATATAGTTTTTCTTTTCAACGTTTGGGGCCCAAGGGACTGCGAATTAGCGAGGGAAGGGTGTTCCATGTCCTATTCCTTACACAGTGTCCTAATATACAGGGTTCAATATACTTGACATATTTGGCTATTTCCGACATTGAATCTGAAGGAAGCTAATAAGGGATTTTGGAGAATCAAATTTTCTTATGATATGATTCATCAAAACGGTCTTAATTACCGTTTATTGACCTGATCCCATGATCTACGTGTTATTATTATTTGTTTCCATTCAGAGAAAATTCATTGAAGTTACCTTTTTCCAACAATAGGTTGATTTGTATAAACTATGACCTTTAGCTTTGGTTTTGGCTTGGGTTTATTATCAGCTGGTAAATTTTCTCATCCTCTGGGCCTACTTTTTGATCAATCTTTTTCTTCGTTCTTTGTTGCTATTAGTACAGTGATTTTGTAGAATTTTTAAATATATGATGAAGAATGATTGCTTGAACTAGCTTGCTTTGCTGCAAGTGATGTTTCTTCTTAATCTCTATTTAGTTGGTTGCTGGAAACTATTGTAGTGTATCAAATCTTTCATACAAAGTTCGTATATCGTTTCTTTCTCATGTATGAATTTGAACCTATAGAGACTGGAGCTCGAGAAGAAAAGGATCATTTATGGAGGCCGACGCAGGAAGAGGTTTGGGATGCCAGAAGACTATGGATGGGAAGGCGAGTAATGGGATTGTTCCAAAGAAGGAGATCCCTTCTTGTTGTTTAAAGGCCATGGCTTCAGCCCCTGAGCTTGATGCCAAGTGCCATTCCACTGTTGTTTCTGGCTGGTTCTCGGAACATCAGTCTGCTTCTGGTAATTAACTATGCTTGTTTCCTTCTATTTTGGTTCTACCATTCACGTGTTCTGATCAGATCCTTCATTGACATGTTTTTGTTTCAATATCTTCCAAATTCAGATAAGGGAAACAAAATGGTCTACTTCAACAATCCCATGTGGCCTGGTACCCTTCTCTACTTTTTTCTTTATCTTTTGATAGCTTATATTATTGCCTATCTGATGAACATCATTCTCTACTTTCATATTTATTCTGTGTTGGTTCTTAAACTTTTAGGCGTTCTTCTTTGGCCGATGAACTTTGCATGAAAACTATTTTGGCCATTGTTCATTTTATGTAAAACATTTGAGGAAAGATCTGTGTTATAGGTGAGAAGTATTTGAAGCTTCTTATGGCAAGCCTAGTTTCACACTACGAAACAATTAGATAATTAATGAAAAACTAACGACAAAGATTCAGAGAGTTTTTGTTATTCAAATATTAAGGATTGATATCTATTTATGTTAAGTTGATCCTTGCTTGAGTGTTCTAACCGACGATAATATGAGTTTTGCATTCCTACCTCAAGCACGACAATTATGGCCTTTATAATTCTGTTTGTTTTTAGGAGAAGCACATTCCCTGCAAGTAGAGTCTATATTATTTCAAGGGAGGTCAGAGTACCAAGAAGTAGTTGTTTTCGAGGTATGTTTTTTGTTTCGTATTGACCCTTTTCGCTTAACATTCTGTGATTTCCTAAATGAAATATAAATTTGAGGTAGCTAATACCACGATTTTTCATTGACAGTCCACTTCATATGGGAAAGTGCTTGTTCTAGATGGTATTGTCCAGTTAACTGAGAAAGATGAGTGTGCCTACCAGGAGATGATAACTCACCTGCCCCTCTGTTCAATTCCATCTCCCAAAACGGTGACTTCATATATTCAAACCATCGTGCTTTCGTCTGTACAGTTGTAGTCTTCCATTTTCTCGTGCAATCAGTATGAGGAATGATGGAATAATTTATTTGTTGTTAAGAGTTTTGATTTTAAAACAAGCAACTGGAATTGTGGTTTGGTAACTATGAGTTAACTTACTTTTGGCATCTGATTGGTTCCGATCTTGACTTGCTTGCCAATGTGAAACTTATATAGTTGCACTTGATATGGGATCTATTAACTATCCAGTTTGATCTGTTTTGATCATTTTGTTCTCCGAGTTCGTTGCTATCTTGGGTTTGCATAAAATAGTTAGCTTGACGAGGTCACGGGTTTTTATATCTGTTTCAAAATCATTTTACACTAAGATTACGTCTTTGTGTTTAGCCGTATGGATTTCTTTCAGCTGTTGAAGATTAAGTTATATATGTTCAATCAAATCCGTTGCAGGTTCTTGTTGTTGGTGGTGGTGATGGTGGAGTTCTAAGGGAAGTCGCTCGCCACAACTCTGTAGAGCATATCGATATATGTGAGATAGATAAGATGGTCATTGATGTATGATATGTAACATCCGCTTTTGTGATAAACTCACTATGTTTTCTCTCAAGTTAATCACCTTGTTGTCCTGGTTTCTTTACCTATAGGTATCCAAGGAGTTCTTTCCCGAGTTAGCTAAAGGATTTGAGGATCCCCGTGTCCACCTTCACGTTGGAGATGGTATGTCCTGATGTAGTTTATCTTTCCTTTTATATATTTTTTTATATCAATTAATTGGATCGCCATTGAGATTATTATGCTATATGTCCAATGTTATCTCTCTGCGTCGTGATTTTTACAGCTGTTGATTTCCTACGGCGTGCACCAAAAGAGAAATACGATGCTATCATAGTTGATTCCTCGGACCCTGTTGGTATGTTCGTAGTTTATATGATATCGAGTTCATGTAACTCATTTATAACAAGTGCATGTCACTGTACGCTCACAAAAGCCATTTCCTGTGGTATCTCTAGTTTAAATAAGGCTGTTGCTATTTTAGGACCTGCTCAGGAGCTAGTTGAGAAGCCATTCTTTGAGACGATAGCAAAAGCATTGAAACCTGGTGGTGTTCTCTGTAACATGGCAGAAAGTATGTGGCTACATACACATCTTATTCAAGATATGATCTCTATTTGCCGTAAAATTTTCAAAGGGTCTGTTCATTATGCATGGGGCAGCGTTCCAACTTATCCAAGGTCACCATAACTTGTAGCACACTGCTTTTAAAGGATTCATCTTGATATTGCCATTTTCAGATGATTATTGCTCTTTCTCAATTCTGGGTTTTGTATTCTTCAGTGGTGTGATAGGTTTTCTGCTATGCTCAACTGAAGGGCCACCTGTTGATTTTAAGAATCCTATCAATCCCATCGAGAAGCTAGAAAGTTCAGCCGAGTATGAAAGAGAGCTCAAGTTCTACAATTCAGAGGTACATTTATCCTCTGCTCCAGCCAGTCTCTTGTAGCATAGATCATGTGGATAAGTTCCTTCAAAGAAATAGAACTGATATGAACTGTTGTTGAAAATGGCAGATACACTCTGCTGCTTTTGCTTTGCCATCGTTTCTGAGGAGGGAGGTGAAGGCGCTTGTTGATTCTCCAGACCCGGCACAGGACTGATTCCGGTAGAGGAGGCGACGTCCAGGTCTACCCATTTCCTGTTTGGTTGGGTTGGTATTTACAAATAATGAAAGTGATTGAACATTGATTCTGAAAACATTTATATTATTGACTTGTAATGGTAGTAGCTATATCTTCTGTGAGATCAAACTTGTTGATGACATCTCATTGAGTTTCTTCTTGCTTATGAGTGATTTGAAGCAGTGCTTTTATAATATGATTTGTGCACTTCTTTGGTGAATGGATGGTGTCATGGAACTACTTTAGAACTTTGGGTATTCGATTAGGAGGTATACGTGATATCATTTAC

mRNA sequence

AGACCAAGCACGAAGCAAAAGAAGAAGGAAACTGGTTCAAGGCCTAAATCTTCTCCCTGTGAGCCATAGTCGAGATTAGGATGGGCCTTTAAATGGTGCATACGTCATACATGCCCTTGGAATAATTTCGTTCGGCTCGATTTGATTATTTGTGTAGAGGTCTGGAATGGAGGAGTTTTGTGGCTCGAGAATCTTTGAGATGGGGTCCTGACTTTCAATTTTTTCAACATTGATCGCGTGCTAAATACAGGTTCGTCCTCGTCGGGTCGCGGCACGATTTCTGATCCGCCCACCTAATCGAGATTCATGATCTTTCTTTCAATTTTCTATATATAAATGTAATGCCCCAAATCCCAATTTTCATCATACTTCAACGAGAATCGGAAACCAGAGCATACAGAGAGAGGAGTGAAACGAAGAGATTGAGTGTGTTTGTGTACGAGAGAATTCTTTTTGCGTCTCTTTCTTGCTTGTTTGCGTCTTTTCCAGAAGTTGGAAGTTCCCATTTCCAGAAATTAACGCCTGGTAATCTCTCTGTCGTTAACCCTTTTTGACTCTGCTTTGATTGTTCTTGCTCTGTAATCTATGGCTTCGCTCTGAGTTTGGTTTGATTGTTGCTTAAATGCATTTTTCTTCTGTTTTGATGGATTCTGTTTGTTTCCTCGGATTTTTTTTGTCGTTTGATTACGGATACATATTGTGGGTATGTATCCCTTCTATCTCTCTCTCTCTCTCTGTCTTCTTGTTCTTCCGATTGTTGAGTTTAATATTGCGTTCGTGCTCAAATACTGCTATCATTTTCACTTTCGTCCTCCTTGCCCTTCAATCTCTCGGACTCAAACTGGAAATTTCGGGCTATAGAATGATTTATCCTTAGTCTTACGATTTTCCCGTTCATTTTTTCGTTTTTGAATCATAGAGCCATCTTGTTTGAGAAATTGTTGGTTAATATTGTTCTGTTTTGATCGAATCGTTGGAGATATATGTATCGCTTTAAATATAGTTTTTCTTTTCAACGTTTGGGGCCCAAGGGACTGCGAATTAGCGAGGGAAGGGTGTTCCATGTCCTATTCCTTACACAGTGTCCTAATATACAGGGTTCAATATACTTGACATATTTGGCTATTTCCGACATTGAATCTGAAGGAAGCTAATAAGGGATTTTGGAGAATCAAATTTTCTTATGATATGATTCATCAAAACGGTCTTAATTACCGTTTATTGACCTGATCCCATGATCTACGTGTTATTATTATTTGTTTCCATTCAGAGAAAATTCATTGAAGTTACCTTTTTCCAACAATAGGTTGATTTGTATAAACTATGACCTTTAGCTTTGGTTTTGGCTTGGGTTTATTATCAGCTGGTAAATTTTCTCATCCTCTGGGCCTACTTTTTGATCAATCTTTTTCTTCGTTCTTTGTTGCTATTAGTACAGTGATTTTGTAGAATTTTTAAATATATGATGAAGAATGATTGCTTGAACTAGCTTGCTTTGCTGCAAGTGATGTTTCTTCTTAATCTCTATTTAGTTGGTTGCTGGAAACTATTGTAGTGTATCAAATCTTTCATACAAAGTTCGTATATCGTTTCTTTCTCATGTATGAATTTGAACCTATAGAGACTGGAGCTCGAGAAGAAAAGGATCATTTATGGAGGCCGACGCAGGAAGAGGTTTGGGATGCCAGAAGACTATGGATGGGAAGGCGAGTAATGGGATTGTTCCAAAGAAGGAGATCCCTTCTTGTTGTTTAAAGGCCATGGCTTCAGCCCCTGAGCTTGATGCCAAGTGCCATTCCACTGTTGTTTCTGGCTGGTTCTCGGAACATCAGTCTGCTTCTGATAAGGGAAACAAAATGGTCTACTTCAACAATCCCATGTGGCCTGGAGAAGCACATTCCCTGCAAGTAGAGTCTATATTATTTCAAGGGAGGTCAGAGTACCAAGAAGTAGTTGTTTTCGAGTCCACTTCATATGGGAAAGTGCTTGTTCTAGATGGTATTGTCCAGTTAACTGAGAAAGATGAGTGTGCCTACCAGGAGATGATAACTCACCTGCCCCTCTGTTCAATTCCATCTCCCAAAACGGTTCTTGTTGTTGGTGGTGGTGATGGTGGAGTTCTAAGGGAAGTCGCTCGCCACAACTCTGTAGAGCATATCGATATATGTGAGATAGATAAGATGGTCATTGATGTATCCAAGGAGTTCTTTCCCGAGTTAGCTAAAGGATTTGAGGATCCCCGTGTCCACCTTCACGTTGGAGATGCTGTTGATTTCCTACGGCGTGCACCAAAAGAGAAATACGATGCTATCATAGTTGATTCCTCGGACCCTGTTGGACCTGCTCAGGAGCTAGTTGAGAAGCCATTCTTTGAGACGATAGCAAAAGCATTGAAACCTGGTGGTGTTCTCTGTAACATGGCAGAAAGTATGTGGCTACATACACATCTTATTCAAGATATGATCTCTATTTGCCGTAAAATTTTCAAAGGGTCTGTTCATTATGCATGGGGCAGCGTTCCAACTTATCCAAGTGGTGTGATAGGTTTTCTGCTATGCTCAACTGAAGGGCCACCTGTTGATTTTAAGAATCCTATCAATCCCATCGAGAAGCTAGAAAGTTCAGCCGAGTATGAAAGAGAGCTCAAGTTCTACAATTCAGAGATACACTCTGCTGCTTTTGCTTTGCCATCGTTTCTGAGGAGGGAGGTGAAGGCGCTTGTTGATTCTCCAGACCCGGCACAGGACTGATTCCGGTAGAGGAGGCGACGTCCAGGTCTACCCATTTCCTGTTTGGTTGGGTTGGTATTTACAAATAATGAAAGTGATTGAACATTGATTCTGAAAACATTTATATTATTGACTTGTAATGGTAGTAGCTATATCTTCTGTGAGATCAAACTTGTTGATGACATCTCATTGAGTTTCTTCTTGCTTATGAGTGATTTGAAGCAGTGCTTTTATAATATGATTTGTGCACTTCTTTGGTGAATGGATGGTGTCATGGAACTACTTTAGAACTTTGGGTATTCGATTAGGAGGTATACGTGATATCATTTAC

Coding sequence (CDS)

ATGGAGGCCGACGCAGGAAGAGGTTTGGGATGCCAGAAGACTATGGATGGGAAGGCGAGTAATGGGATTGTTCCAAAGAAGGAGATCCCTTCTTGTTGTTTAAAGGCCATGGCTTCAGCCCCTGAGCTTGATGCCAAGTGCCATTCCACTGTTGTTTCTGGCTGGTTCTCGGAACATCAGTCTGCTTCTGATAAGGGAAACAAAATGGTCTACTTCAACAATCCCATGTGGCCTGGAGAAGCACATTCCCTGCAAGTAGAGTCTATATTATTTCAAGGGAGGTCAGAGTACCAAGAAGTAGTTGTTTTCGAGTCCACTTCATATGGGAAAGTGCTTGTTCTAGATGGTATTGTCCAGTTAACTGAGAAAGATGAGTGTGCCTACCAGGAGATGATAACTCACCTGCCCCTCTGTTCAATTCCATCTCCCAAAACGGTTCTTGTTGTTGGTGGTGGTGATGGTGGAGTTCTAAGGGAAGTCGCTCGCCACAACTCTGTAGAGCATATCGATATATGTGAGATAGATAAGATGGTCATTGATGTATCCAAGGAGTTCTTTCCCGAGTTAGCTAAAGGATTTGAGGATCCCCGTGTCCACCTTCACGTTGGAGATGCTGTTGATTTCCTACGGCGTGCACCAAAAGAGAAATACGATGCTATCATAGTTGATTCCTCGGACCCTGTTGGACCTGCTCAGGAGCTAGTTGAGAAGCCATTCTTTGAGACGATAGCAAAAGCATTGAAACCTGGTGGTGTTCTCTGTAACATGGCAGAAAGTATGTGGCTACATACACATCTTATTCAAGATATGATCTCTATTTGCCGTAAAATTTTCAAAGGGTCTGTTCATTATGCATGGGGCAGCGTTCCAACTTATCCAAGTGGTGTGATAGGTTTTCTGCTATGCTCAACTGAAGGGCCACCTGTTGATTTTAAGAATCCTATCAATCCCATCGAGAAGCTAGAAAGTTCAGCCGAGTATGAAAGAGAGCTCAAGTTCTACAATTCAGAGATACACTCTGCTGCTTTTGCTTTGCCATCGTTTCTGAGGAGGGAGGTGAAGGCGCTTGTTGATTCTCCAGACCCGGCACAGGACTGA

Protein sequence

MEADAGRGLGCQKTMDGKASNGIVPKKEIPSCCLKAMASAPELDAKCHSTVVSGWFSEHQSASDKGNKMVYFNNPMWPGEAHSLQVESILFQGRSEYQEVVVFESTSYGKVLVLDGIVQLTEKDECAYQEMITHLPLCSIPSPKTVLVVGGGDGGVLREVARHNSVEHIDICEIDKMVIDVSKEFFPELAKGFEDPRVHLHVGDAVDFLRRAPKEKYDAIIVDSSDPVGPAQELVEKPFFETIAKALKPGGVLCNMAESMWLHTHLIQDMISICRKIFKGSVHYAWGSVPTYPSGVIGFLLCSTEGPPVDFKNPINPIEKLESSAEYERELKFYNSEIHSAAFALPSFLRREVKALVDSPDPAQD
Homology
BLAST of Carg14021 vs. NCBI nr
Match: KAG7035744.1 (Spermine synthase [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 750.7 bits (1937), Expect = 5.7e-213
Identity = 365/365 (100.00%), Postives = 365/365 (100.00%), Query Frame = 0

Query: 1   MEADAGRGLGCQKTMDGKASNGIVPKKEIPSCCLKAMASAPELDAKCHSTVVSGWFSEHQ 60
           MEADAGRGLGCQKTMDGKASNGIVPKKEIPSCCLKAMASAPELDAKCHSTVVSGWFSEHQ
Sbjct: 1   MEADAGRGLGCQKTMDGKASNGIVPKKEIPSCCLKAMASAPELDAKCHSTVVSGWFSEHQ 60

Query: 61  SASDKGNKMVYFNNPMWPGEAHSLQVESILFQGRSEYQEVVVFESTSYGKVLVLDGIVQL 120
           SASDKGNKMVYFNNPMWPGEAHSLQVESILFQGRSEYQEVVVFESTSYGKVLVLDGIVQL
Sbjct: 61  SASDKGNKMVYFNNPMWPGEAHSLQVESILFQGRSEYQEVVVFESTSYGKVLVLDGIVQL 120

Query: 121 TEKDECAYQEMITHLPLCSIPSPKTVLVVGGGDGGVLREVARHNSVEHIDICEIDKMVID 180
           TEKDECAYQEMITHLPLCSIPSPKTVLVVGGGDGGVLREVARHNSVEHIDICEIDKMVID
Sbjct: 121 TEKDECAYQEMITHLPLCSIPSPKTVLVVGGGDGGVLREVARHNSVEHIDICEIDKMVID 180

Query: 181 VSKEFFPELAKGFEDPRVHLHVGDAVDFLRRAPKEKYDAIIVDSSDPVGPAQELVEKPFF 240
           VSKEFFPELAKGFEDPRVHLHVGDAVDFLRRAPKEKYDAIIVDSSDPVGPAQELVEKPFF
Sbjct: 181 VSKEFFPELAKGFEDPRVHLHVGDAVDFLRRAPKEKYDAIIVDSSDPVGPAQELVEKPFF 240

Query: 241 ETIAKALKPGGVLCNMAESMWLHTHLIQDMISICRKIFKGSVHYAWGSVPTYPSGVIGFL 300
           ETIAKALKPGGVLCNMAESMWLHTHLIQDMISICRKIFKGSVHYAWGSVPTYPSGVIGFL
Sbjct: 241 ETIAKALKPGGVLCNMAESMWLHTHLIQDMISICRKIFKGSVHYAWGSVPTYPSGVIGFL 300

Query: 301 LCSTEGPPVDFKNPINPIEKLESSAEYERELKFYNSEIHSAAFALPSFLRREVKALVDSP 360
           LCSTEGPPVDFKNPINPIEKLESSAEYERELKFYNSEIHSAAFALPSFLRREVKALVDSP
Sbjct: 301 LCSTEGPPVDFKNPINPIEKLESSAEYERELKFYNSEIHSAAFALPSFLRREVKALVDSP 360

Query: 361 DPAQD 366
           DPAQD
Sbjct: 361 DPAQD 365

BLAST of Carg14021 vs. NCBI nr
Match: XP_022957976.1 (spermine synthase-like isoform X2 [Cucurbita moschata])

HSP 1 Score: 749.2 bits (1933), Expect = 1.7e-212
Identity = 364/365 (99.73%), Postives = 365/365 (100.00%), Query Frame = 0

Query: 1   MEADAGRGLGCQKTMDGKASNGIVPKKEIPSCCLKAMASAPELDAKCHSTVVSGWFSEHQ 60
           MEADAGRGLGCQKTMDGKASNGIVPKKEIPSCCLKAMASAPELDAKCHSTVVSGWFSEHQ
Sbjct: 1   MEADAGRGLGCQKTMDGKASNGIVPKKEIPSCCLKAMASAPELDAKCHSTVVSGWFSEHQ 60

Query: 61  SASDKGNKMVYFNNPMWPGEAHSLQVESILFQGRSEYQEVVVFESTSYGKVLVLDGIVQL 120
           SASDKGNKMVYFNNPMWPGEAHSLQVESILFQGRSEYQEVVVF+STSYGKVLVLDGIVQL
Sbjct: 61  SASDKGNKMVYFNNPMWPGEAHSLQVESILFQGRSEYQEVVVFKSTSYGKVLVLDGIVQL 120

Query: 121 TEKDECAYQEMITHLPLCSIPSPKTVLVVGGGDGGVLREVARHNSVEHIDICEIDKMVID 180
           TEKDECAYQEMITHLPLCSIPSPKTVLVVGGGDGGVLREVARHNSVEHIDICEIDKMVID
Sbjct: 121 TEKDECAYQEMITHLPLCSIPSPKTVLVVGGGDGGVLREVARHNSVEHIDICEIDKMVID 180

Query: 181 VSKEFFPELAKGFEDPRVHLHVGDAVDFLRRAPKEKYDAIIVDSSDPVGPAQELVEKPFF 240
           VSKEFFPELAKGFEDPRVHLHVGDAVDFLRRAPKEKYDAIIVDSSDPVGPAQELVEKPFF
Sbjct: 181 VSKEFFPELAKGFEDPRVHLHVGDAVDFLRRAPKEKYDAIIVDSSDPVGPAQELVEKPFF 240

Query: 241 ETIAKALKPGGVLCNMAESMWLHTHLIQDMISICRKIFKGSVHYAWGSVPTYPSGVIGFL 300
           ETIAKALKPGGVLCNMAESMWLHTHLIQDMISICRKIFKGSVHYAWGSVPTYPSGVIGFL
Sbjct: 241 ETIAKALKPGGVLCNMAESMWLHTHLIQDMISICRKIFKGSVHYAWGSVPTYPSGVIGFL 300

Query: 301 LCSTEGPPVDFKNPINPIEKLESSAEYERELKFYNSEIHSAAFALPSFLRREVKALVDSP 360
           LCSTEGPPVDFKNPINPIEKLESSAEYERELKFYNSEIHSAAFALPSFLRREVKALVDSP
Sbjct: 301 LCSTEGPPVDFKNPINPIEKLESSAEYERELKFYNSEIHSAAFALPSFLRREVKALVDSP 360

Query: 361 DPAQD 366
           DPAQD
Sbjct: 361 DPAQD 365

BLAST of Carg14021 vs. NCBI nr
Match: XP_022995840.1 (spermine synthase-like isoform X2 [Cucurbita maxima] >XP_022995841.1 spermine synthase-like isoform X2 [Cucurbita maxima])

HSP 1 Score: 745.3 bits (1923), Expect = 2.4e-211
Identity = 361/365 (98.90%), Postives = 363/365 (99.45%), Query Frame = 0

Query: 1   MEADAGRGLGCQKTMDGKASNGIVPKKEIPSCCLKAMASAPELDAKCHSTVVSGWFSEHQ 60
           MEADAGRGLGCQKTMDGKASNGIVPKKEIPSCCLKAMASAPELDAKCHSTVVSGWFSEHQ
Sbjct: 1   MEADAGRGLGCQKTMDGKASNGIVPKKEIPSCCLKAMASAPELDAKCHSTVVSGWFSEHQ 60

Query: 61  SASDKGNKMVYFNNPMWPGEAHSLQVESILFQGRSEYQEVVVFESTSYGKVLVLDGIVQL 120
           SASDKGNKMVYFNNPMWPGEAHSLQVESILFQ RSEYQEVVVFESTSYGKVLVLDGIVQL
Sbjct: 61  SASDKGNKMVYFNNPMWPGEAHSLQVESILFQRRSEYQEVVVFESTSYGKVLVLDGIVQL 120

Query: 121 TEKDECAYQEMITHLPLCSIPSPKTVLVVGGGDGGVLREVARHNSVEHIDICEIDKMVID 180
           TEKDECAYQEMITHLPLCSIPSPKTVLVVGGGDGGVLREVARHNSVEHIDICEIDKMVID
Sbjct: 121 TEKDECAYQEMITHLPLCSIPSPKTVLVVGGGDGGVLREVARHNSVEHIDICEIDKMVID 180

Query: 181 VSKEFFPELAKGFEDPRVHLHVGDAVDFLRRAPKEKYDAIIVDSSDPVGPAQELVEKPFF 240
           VSKEFFPELAKGFEDPRVHLHVGDA+DFLRRAPKEKYDA+IVDSSDPVGPAQELVEKPFF
Sbjct: 181 VSKEFFPELAKGFEDPRVHLHVGDAIDFLRRAPKEKYDAVIVDSSDPVGPAQELVEKPFF 240

Query: 241 ETIAKALKPGGVLCNMAESMWLHTHLIQDMISICRKIFKGSVHYAWGSVPTYPSGVIGFL 300
           ETIAKALKPGGVLCNMAESMWLHTHLIQDMISICRKIFKGSVHYAWGSVPTYPSGVIGFL
Sbjct: 241 ETIAKALKPGGVLCNMAESMWLHTHLIQDMISICRKIFKGSVHYAWGSVPTYPSGVIGFL 300

Query: 301 LCSTEGPPVDFKNPINPIEKLESSAEYERELKFYNSEIHSAAFALPSFLRREVKALVDSP 360
           LCSTEGPPVDFKNPINPIEKLE SAEYERELKFYNSEIHSAAFALPSFLRREVKALVDSP
Sbjct: 301 LCSTEGPPVDFKNPINPIEKLEGSAEYERELKFYNSEIHSAAFALPSFLRREVKALVDSP 360

Query: 361 DPAQD 366
           DPAQD
Sbjct: 361 DPAQD 365

BLAST of Carg14021 vs. NCBI nr
Match: XP_022957972.1 (spermine synthase-like isoform X1 [Cucurbita moschata] >XP_022957974.1 spermine synthase-like isoform X1 [Cucurbita moschata] >XP_022957975.1 spermine synthase-like isoform X1 [Cucurbita moschata])

HSP 1 Score: 744.2 bits (1920), Expect = 5.4e-211
Identity = 364/367 (99.18%), Postives = 365/367 (99.46%), Query Frame = 0

Query: 1   MEADAGRGLGCQKTMDGKASNGIVPKKEIPSCCLKAMASAPELDAKCHSTVVSGWFSEHQ 60
           MEADAGRGLGCQKTMDGKASNGIVPKKEIPSCCLKAMASAPELDAKCHSTVVSGWFSEHQ
Sbjct: 1   MEADAGRGLGCQKTMDGKASNGIVPKKEIPSCCLKAMASAPELDAKCHSTVVSGWFSEHQ 60

Query: 61  SA--SDKGNKMVYFNNPMWPGEAHSLQVESILFQGRSEYQEVVVFESTSYGKVLVLDGIV 120
           SA  SDKGNKMVYFNNPMWPGEAHSLQVESILFQGRSEYQEVVVF+STSYGKVLVLDGIV
Sbjct: 61  SASDSDKGNKMVYFNNPMWPGEAHSLQVESILFQGRSEYQEVVVFKSTSYGKVLVLDGIV 120

Query: 121 QLTEKDECAYQEMITHLPLCSIPSPKTVLVVGGGDGGVLREVARHNSVEHIDICEIDKMV 180
           QLTEKDECAYQEMITHLPLCSIPSPKTVLVVGGGDGGVLREVARHNSVEHIDICEIDKMV
Sbjct: 121 QLTEKDECAYQEMITHLPLCSIPSPKTVLVVGGGDGGVLREVARHNSVEHIDICEIDKMV 180

Query: 181 IDVSKEFFPELAKGFEDPRVHLHVGDAVDFLRRAPKEKYDAIIVDSSDPVGPAQELVEKP 240
           IDVSKEFFPELAKGFEDPRVHLHVGDAVDFLRRAPKEKYDAIIVDSSDPVGPAQELVEKP
Sbjct: 181 IDVSKEFFPELAKGFEDPRVHLHVGDAVDFLRRAPKEKYDAIIVDSSDPVGPAQELVEKP 240

Query: 241 FFETIAKALKPGGVLCNMAESMWLHTHLIQDMISICRKIFKGSVHYAWGSVPTYPSGVIG 300
           FFETIAKALKPGGVLCNMAESMWLHTHLIQDMISICRKIFKGSVHYAWGSVPTYPSGVIG
Sbjct: 241 FFETIAKALKPGGVLCNMAESMWLHTHLIQDMISICRKIFKGSVHYAWGSVPTYPSGVIG 300

Query: 301 FLLCSTEGPPVDFKNPINPIEKLESSAEYERELKFYNSEIHSAAFALPSFLRREVKALVD 360
           FLLCSTEGPPVDFKNPINPIEKLESSAEYERELKFYNSEIHSAAFALPSFLRREVKALVD
Sbjct: 301 FLLCSTEGPPVDFKNPINPIEKLESSAEYERELKFYNSEIHSAAFALPSFLRREVKALVD 360

Query: 361 SPDPAQD 366
           SPDPAQD
Sbjct: 361 SPDPAQD 367

BLAST of Carg14021 vs. NCBI nr
Match: XP_022995836.1 (spermine synthase-like isoform X1 [Cucurbita maxima] >XP_022995837.1 spermine synthase-like isoform X1 [Cucurbita maxima] >XP_022995838.1 spermine synthase-like isoform X1 [Cucurbita maxima] >XP_022995839.1 spermine synthase-like isoform X1 [Cucurbita maxima])

HSP 1 Score: 740.3 bits (1910), Expect = 7.8e-210
Identity = 361/367 (98.37%), Postives = 363/367 (98.91%), Query Frame = 0

Query: 1   MEADAGRGLGCQKTMDGKASNGIVPKKEIPSCCLKAMASAPELDAKCHSTVVSGWFSEHQ 60
           MEADAGRGLGCQKTMDGKASNGIVPKKEIPSCCLKAMASAPELDAKCHSTVVSGWFSEHQ
Sbjct: 1   MEADAGRGLGCQKTMDGKASNGIVPKKEIPSCCLKAMASAPELDAKCHSTVVSGWFSEHQ 60

Query: 61  SA--SDKGNKMVYFNNPMWPGEAHSLQVESILFQGRSEYQEVVVFESTSYGKVLVLDGIV 120
           SA  SDKGNKMVYFNNPMWPGEAHSLQVESILFQ RSEYQEVVVFESTSYGKVLVLDGIV
Sbjct: 61  SASDSDKGNKMVYFNNPMWPGEAHSLQVESILFQRRSEYQEVVVFESTSYGKVLVLDGIV 120

Query: 121 QLTEKDECAYQEMITHLPLCSIPSPKTVLVVGGGDGGVLREVARHNSVEHIDICEIDKMV 180
           QLTEKDECAYQEMITHLPLCSIPSPKTVLVVGGGDGGVLREVARHNSVEHIDICEIDKMV
Sbjct: 121 QLTEKDECAYQEMITHLPLCSIPSPKTVLVVGGGDGGVLREVARHNSVEHIDICEIDKMV 180

Query: 181 IDVSKEFFPELAKGFEDPRVHLHVGDAVDFLRRAPKEKYDAIIVDSSDPVGPAQELVEKP 240
           IDVSKEFFPELAKGFEDPRVHLHVGDA+DFLRRAPKEKYDA+IVDSSDPVGPAQELVEKP
Sbjct: 181 IDVSKEFFPELAKGFEDPRVHLHVGDAIDFLRRAPKEKYDAVIVDSSDPVGPAQELVEKP 240

Query: 241 FFETIAKALKPGGVLCNMAESMWLHTHLIQDMISICRKIFKGSVHYAWGSVPTYPSGVIG 300
           FFETIAKALKPGGVLCNMAESMWLHTHLIQDMISICRKIFKGSVHYAWGSVPTYPSGVIG
Sbjct: 241 FFETIAKALKPGGVLCNMAESMWLHTHLIQDMISICRKIFKGSVHYAWGSVPTYPSGVIG 300

Query: 301 FLLCSTEGPPVDFKNPINPIEKLESSAEYERELKFYNSEIHSAAFALPSFLRREVKALVD 360
           FLLCSTEGPPVDFKNPINPIEKLE SAEYERELKFYNSEIHSAAFALPSFLRREVKALVD
Sbjct: 301 FLLCSTEGPPVDFKNPINPIEKLEGSAEYERELKFYNSEIHSAAFALPSFLRREVKALVD 360

Query: 361 SPDPAQD 366
           SPDPAQD
Sbjct: 361 SPDPAQD 367

BLAST of Carg14021 vs. ExPASy Swiss-Prot
Match: Q94BN2 (Spermine synthase OS=Arabidopsis thaliana OX=3702 GN=SPMS PE=1 SV=1)

HSP 1 Score: 588.6 bits (1516), Expect = 4.9e-167
Identity = 283/360 (78.61%), Postives = 318/360 (88.33%), Query Frame = 0

Query: 1   MEADAGRGLGCQKTMDGKASNGIVPKKEIPSCCLKAMASAPELDAKCHSTVVSGWFSE-H 60
           ME D G GL CQ TMDGKASNG   +K +PSCCLKAMA  PE DAKCHSTVVSGWFSE H
Sbjct: 1   MEGDVGIGLVCQNTMDGKASNGNGLEKTVPSCCLKAMACVPEDDAKCHSTVVSGWFSEPH 60

Query: 61  QSASDKGNKMVYFNNPMWPGEAHSLQVESILFQGRSEYQEVVVFESTSYGKVLVLDGIVQ 120
             +  KG K VYFNNPMWPGEAHSL+VE +LF+ +S++QEV+VFES +YGKVLVLDGIVQ
Sbjct: 61  PRSGKKGGKAVYFNNPMWPGEAHSLKVEKVLFKDKSDFQEVLVFESATYGKVLVLDGIVQ 120

Query: 121 LTEKDECAYQEMITHLPLCSIPSPKTVLVVGGGDGGVLREVARHNSVEHIDICEIDKMVI 180
           LTEKDECAYQEMI HLPLCSI SPK VLVVGGGDGGVLRE++RH+SVE IDICEIDKMVI
Sbjct: 121 LTEKDECAYQEMIAHLPLCSISSPKNVLVVGGGDGGVLREISRHSSVEVIDICEIDKMVI 180

Query: 181 DVSKEFFPELAKGFEDPRVHLHVGDAVDFLRRAPKEKYDAIIVDSSDPVGPAQELVEKPF 240
           DVSK+FFPELA GF+DPRV LH+GDA +FLR++P+ KYDAIIVDSSDPVGPA  LVEKPF
Sbjct: 181 DVSKKFFPELAVGFDDPRVQLHIGDAAEFLRKSPEGKYDAIIVDSSDPVGPALALVEKPF 240

Query: 241 FETIAKALKPGGVLCNMAESMWLHTHLIQDMISICRKIFKGSVHYAWGSVPTYPSGVIGF 300
           FET+A+ALKPGGVLCNMAESMWLHTHLI+DMISICR+ FK SVHYAW SVPTYPSGVIGF
Sbjct: 241 FETLARALKPGGVLCNMAESMWLHTHLIEDMISICRQTFK-SVHYAWSSVPTYPSGVIGF 300

Query: 301 LLCSTEGPPVDFKNPINPIEKLESSAEYERELKFYNSEIHSAAFALPSFLRREVKALVDS 360
           +LCSTEGP VDFKNPINPIEKL+ +  ++RELKFYNS++H AAFALP+FLRREV +L+ S
Sbjct: 301 VLCSTEGPAVDFKNPINPIEKLDGAMTHKRELKFYNSDMHRAAFALPTFLRREVASLLAS 359

BLAST of Carg14021 vs. ExPASy Swiss-Prot
Match: O82147 (Spermidine synthase OS=Coffea arabica OX=13443 PE=2 SV=1)

HSP 1 Score: 463.0 bits (1190), Expect = 3.1e-129
Identity = 214/305 (70.16%), Postives = 259/305 (84.92%), Query Frame = 0

Query: 49  STVVSGWFSEHQSASDKGNKMVYFNNPMWPGEAHSLQVESILFQGRSEYQEVVVFESTSY 108
           S+V+ GWFSE               +PMWPGEAHSL+VE ILFQG+S+YQ V+VF+S++Y
Sbjct: 21  SSVIPGWFSE--------------ISPMWPGEAHSLKVEKILFQGKSDYQNVMVFQSSTY 80

Query: 109 GKVLVLDGIVQLTEKDECAYQEMITHLPLCSIPSPKTVLVVGGGDGGVLREVARHNSVEH 168
           GKVLVLDG++QLTE+DECAYQEMI HLPLCSIPSPK VLV+GGGDGGVLREVARH SVE 
Sbjct: 81  GKVLVLDGVIQLTERDECAYQEMIAHLPLCSIPSPKKVLVIGGGDGGVLREVARHLSVEQ 140

Query: 169 IDICEIDKMVIDVSKEFFPELAKGFEDPRVHLHVGDAVDFLRRAPKEKYDAIIVDSSDPV 228
           IDICEIDKMV+DVSK+FFP++A GFEDPRV LH+GD V FL+  P+  YDAIIVDSSDP+
Sbjct: 141 IDICEIDKMVVDVSKQFFPDVAVGFEDPRVVLHIGDGVAFLKAVPEGTYDAIIVDSSDPI 200

Query: 229 GPAQELVEKPFFETIAKALKPGGVLCNMAESMWLHTHLIQDMISICRKIFKGSVHYAWGS 288
           GPAQEL EKPFFE++AKAL+PGGV+C  AES+WLH H+I+D+++ CR+IFKGSV+YAW +
Sbjct: 201 GPAQELFEKPFFESVAKALRPGGVVCTQAESIWLHMHIIEDIVANCRQIFKGSVNYAWTT 260

Query: 289 VPTYPSGVIGFLLCSTEGPPVDFKNPINPIEKLESSAEYERELKFYNSEIHSAAFALPSF 348
           VPTYPSGVIGF+LCSTEGPPVDFK+PINPI+  +  ++  + LKFYNSEIHSAAF LPSF
Sbjct: 261 VPTYPSGVIGFMLCSTEGPPVDFKHPINPIDANDGRSKTMKPLKFYNSEIHSAAFCLPSF 311

Query: 349 LRREV 354
            ++ +
Sbjct: 321 AKKVI 311

BLAST of Carg14021 vs. ExPASy Swiss-Prot
Match: O48659 (Spermidine synthase 2 OS=Hyoscyamus niger OX=4079 PE=2 SV=1)

HSP 1 Score: 459.9 bits (1182), Expect = 2.7e-128
Identity = 213/307 (69.38%), Postives = 259/307 (84.36%), Query Frame = 0

Query: 49  STVVSGWFSEHQSASDKGNKMVYFNNPMWPGEAHSLQVESILFQGRSEYQEVVVFESTSY 108
           S+++ GWFSE               +P+WPGEAHSL+VE ILFQG+S+YQ VVVF+S++Y
Sbjct: 13  SSILPGWFSE--------------ISPLWPGEAHSLKVEKILFQGKSDYQNVVVFQSSTY 72

Query: 109 GKVLVLDGIVQLTEKDECAYQEMITHLPLCSIPSPKTVLVVGGGDGGVLREVARHNSVEH 168
           GKVLVLDG++QLTE+DECAYQEMITHLPLCSIP+PK VLV+GGGDGGVLREV+RH+SVE 
Sbjct: 73  GKVLVLDGVIQLTERDECAYQEMITHLPLCSIPNPKKVLVIGGGDGGVLREVSRHSSVEQ 132

Query: 169 IDICEIDKMVIDVSKEFFPELAKGFEDPRVHLHVGDAVDFLRRAPKEKYDAIIVDSSDPV 228
           IDICEIDKMVIDVSK+FFP +A G+EDPRV LHVGD V FL+  P+  YDA+IVDSSDP+
Sbjct: 133 IDICEIDKMVIDVSKQFFPNVAIGYEDPRVKLHVGDGVAFLKNVPEGTYDAVIVDSSDPI 192

Query: 229 GPAQELVEKPFFETIAKALKPGGVLCNMAESMWLHTHLIQDMISICRKIFKGSVHYAWGS 288
           GPAQEL EKPFFE++A+AL PGGV+C  AES+WLH H+I+D++S CR+IFKGSV+YAW +
Sbjct: 193 GPAQELFEKPFFESVARALCPGGVVCTQAESIWLHMHIIEDIVSNCRQIFKGSVNYAWTT 252

Query: 289 VPTYPSGVIGFLLCSTEGPPVDFKNPINPIEKLESSAEYERELKFYNSEIHSAAFALPSF 348
           VPTYPSGVIGF+LCSTEGP VDFKNPINPI+  +S  +    LKFYNSEIHSA+F LPSF
Sbjct: 253 VPTYPSGVIGFMLCSTEGPAVDFKNPINPIDADDSHTKTRGPLKFYNSEIHSASFCLPSF 305

Query: 349 LRREVKA 356
            +R +++
Sbjct: 313 AKRVIES 305

BLAST of Carg14021 vs. ExPASy Swiss-Prot
Match: Q96556 (Spermidine synthase 1 OS=Datura stramonium OX=4076 PE=2 SV=1)

HSP 1 Score: 454.5 bits (1168), Expect = 1.1e-126
Identity = 210/319 (65.83%), Postives = 263/319 (82.45%), Query Frame = 0

Query: 37  MASAPELDAKCHSTVVSGWFSEHQSASDKGNKMVYFNNPMWPGEAHSLQVESILFQGRSE 96
           M  A   ++   S+++ GWFSE               +P+WPGEAHSL+VE ILFQG+S+
Sbjct: 1   MEEANNKESPYISSILPGWFSE--------------ISPLWPGEAHSLKVEKILFQGKSD 60

Query: 97  YQEVVVFESTSYGKVLVLDGIVQLTEKDECAYQEMITHLPLCSIPSPKTVLVVGGGDGGV 156
           YQ V+VF+S++YGKVL+LDG++QLTE+DECAYQEMITHLPLCSIP+PK VLV+GGGDGGV
Sbjct: 61  YQNVMVFQSSTYGKVLILDGVIQLTERDECAYQEMITHLPLCSIPNPKKVLVIGGGDGGV 120

Query: 157 LREVARHNSVEHIDICEIDKMVIDVSKEFFPELAKGFEDPRVHLHVGDAVDFLRRAPKEK 216
           LREV+RH+SVE IDICEIDKMVIDVSK+FFP +A G+EDPRV LHVGD V FL+   +  
Sbjct: 121 LREVSRHSSVEQIDICEIDKMVIDVSKQFFPNVAIGYEDPRVKLHVGDGVAFLKFVAEGT 180

Query: 217 YDAIIVDSSDPVGPAQELVEKPFFETIAKALKPGGVLCNMAESMWLHTHLIQDMISICRK 276
           YDA+IVDSSDP+GPAQEL EKPFFE++A+AL+PGGV+C  AES+WLH H+I+D+++ CR+
Sbjct: 181 YDAVIVDSSDPIGPAQELFEKPFFESVARALRPGGVVCTQAESIWLHMHIIEDIVANCRQ 240

Query: 277 IFKGSVHYAWGSVPTYPSGVIGFLLCSTEGPPVDFKNPINPIEKLESSAEYERELKFYNS 336
           IFKGSV+YAW +VPTYPSGVIGF+LCSTEGP VDFKNPINP++  +S  +    LKFYNS
Sbjct: 241 IFKGSVNYAWTTVPTYPSGVIGFMLCSTEGPAVDFKNPINPVDADDSHTKTRGPLKFYNS 300

Query: 337 EIHSAAFALPSFLRREVKA 356
           EIHSA+F LPSF +R +++
Sbjct: 301 EIHSASFCLPSFAKRVIES 305

BLAST of Carg14021 vs. ExPASy Swiss-Prot
Match: Q9ZUB3 (Spermidine synthase 1 OS=Arabidopsis thaliana OX=3702 GN=SPDSYN1 PE=1 SV=1)

HSP 1 Score: 454.1 bits (1167), Expect = 1.5e-126
Identity = 208/309 (67.31%), Postives = 262/309 (84.79%), Query Frame = 0

Query: 47  CHSTVVSGWFSEHQSASDKGNKMVYFNNPMWPGEAHSLQVESILFQGRSEYQEVVVFEST 106
           C STV+ GWFSE               +PMWPGEAHSL+VE +LFQG+S+YQ+V+VF+S 
Sbjct: 39  CFSTVIPGWFSE--------------MSPMWPGEAHSLKVEKVLFQGKSDYQDVIVFQSA 98

Query: 107 SYGKVLVLDGIVQLTEKDECAYQEMITHLPLCSIPSPKTVLVVGGGDGGVLREVARHNSV 166
           +YGKVLVLDG++QLTE+DECAYQEMITHLPLCSIP+PK VLV+GGGDGGVLREVARH S+
Sbjct: 99  TYGKVLVLDGVIQLTERDECAYQEMITHLPLCSIPNPKKVLVIGGGDGGVLREVARHASI 158

Query: 167 EHIDICEIDKMVIDVSKEFFPELAKGFEDPRVHLHVGDAVDFLRRAPKEKYDAIIVDSSD 226
           E ID+CEIDKMV+DVSK+FFP++A G+EDPRV+L +GD V FL+ A +  YDA+IVDSSD
Sbjct: 159 EQIDMCEIDKMVVDVSKQFFPDVAIGYEDPRVNLVIGDGVAFLKNAAEGSYDAVIVDSSD 218

Query: 227 PVGPAQELVEKPFFETIAKALKPGGVLCNMAESMWLHTHLIQDMISICRKIFKGSVHYAW 286
           P+GPA+EL EKPFF+++A+AL+PGGV+C  AES+WLH  +I+D++S CR+IFKGSV+YAW
Sbjct: 219 PIGPAKELFEKPFFQSVARALRPGGVVCTQAESLWLHMDIIEDIVSNCREIFKGSVNYAW 278

Query: 287 GSVPTYPSGVIGFLLCSTEGPPVDFKNPINPIEKLESSAEYERELKFYNSEIHSAAFALP 346
            SVPTYPSGVIGF+LCSTEGP VDFK+P+NPI+  ESS++    LKFYN+EIHSAAF LP
Sbjct: 279 TSVPTYPSGVIGFMLCSTEGPDVDFKHPLNPID--ESSSKSNGPLKFYNAEIHSAAFCLP 331

Query: 347 SFLRREVKA 356
           SF ++ +++
Sbjct: 339 SFAKKVIES 331

BLAST of Carg14021 vs. ExPASy TrEMBL
Match: A0A6J1H0Q5 (spermine synthase-like isoform X2 OS=Cucurbita moschata OX=3662 GN=LOC111459343 PE=3 SV=1)

HSP 1 Score: 749.2 bits (1933), Expect = 8.1e-213
Identity = 364/365 (99.73%), Postives = 365/365 (100.00%), Query Frame = 0

Query: 1   MEADAGRGLGCQKTMDGKASNGIVPKKEIPSCCLKAMASAPELDAKCHSTVVSGWFSEHQ 60
           MEADAGRGLGCQKTMDGKASNGIVPKKEIPSCCLKAMASAPELDAKCHSTVVSGWFSEHQ
Sbjct: 1   MEADAGRGLGCQKTMDGKASNGIVPKKEIPSCCLKAMASAPELDAKCHSTVVSGWFSEHQ 60

Query: 61  SASDKGNKMVYFNNPMWPGEAHSLQVESILFQGRSEYQEVVVFESTSYGKVLVLDGIVQL 120
           SASDKGNKMVYFNNPMWPGEAHSLQVESILFQGRSEYQEVVVF+STSYGKVLVLDGIVQL
Sbjct: 61  SASDKGNKMVYFNNPMWPGEAHSLQVESILFQGRSEYQEVVVFKSTSYGKVLVLDGIVQL 120

Query: 121 TEKDECAYQEMITHLPLCSIPSPKTVLVVGGGDGGVLREVARHNSVEHIDICEIDKMVID 180
           TEKDECAYQEMITHLPLCSIPSPKTVLVVGGGDGGVLREVARHNSVEHIDICEIDKMVID
Sbjct: 121 TEKDECAYQEMITHLPLCSIPSPKTVLVVGGGDGGVLREVARHNSVEHIDICEIDKMVID 180

Query: 181 VSKEFFPELAKGFEDPRVHLHVGDAVDFLRRAPKEKYDAIIVDSSDPVGPAQELVEKPFF 240
           VSKEFFPELAKGFEDPRVHLHVGDAVDFLRRAPKEKYDAIIVDSSDPVGPAQELVEKPFF
Sbjct: 181 VSKEFFPELAKGFEDPRVHLHVGDAVDFLRRAPKEKYDAIIVDSSDPVGPAQELVEKPFF 240

Query: 241 ETIAKALKPGGVLCNMAESMWLHTHLIQDMISICRKIFKGSVHYAWGSVPTYPSGVIGFL 300
           ETIAKALKPGGVLCNMAESMWLHTHLIQDMISICRKIFKGSVHYAWGSVPTYPSGVIGFL
Sbjct: 241 ETIAKALKPGGVLCNMAESMWLHTHLIQDMISICRKIFKGSVHYAWGSVPTYPSGVIGFL 300

Query: 301 LCSTEGPPVDFKNPINPIEKLESSAEYERELKFYNSEIHSAAFALPSFLRREVKALVDSP 360
           LCSTEGPPVDFKNPINPIEKLESSAEYERELKFYNSEIHSAAFALPSFLRREVKALVDSP
Sbjct: 301 LCSTEGPPVDFKNPINPIEKLESSAEYERELKFYNSEIHSAAFALPSFLRREVKALVDSP 360

Query: 361 DPAQD 366
           DPAQD
Sbjct: 361 DPAQD 365

BLAST of Carg14021 vs. ExPASy TrEMBL
Match: A0A6J1K516 (spermine synthase-like isoform X2 OS=Cucurbita maxima OX=3661 GN=LOC111491249 PE=3 SV=1)

HSP 1 Score: 745.3 bits (1923), Expect = 1.2e-211
Identity = 361/365 (98.90%), Postives = 363/365 (99.45%), Query Frame = 0

Query: 1   MEADAGRGLGCQKTMDGKASNGIVPKKEIPSCCLKAMASAPELDAKCHSTVVSGWFSEHQ 60
           MEADAGRGLGCQKTMDGKASNGIVPKKEIPSCCLKAMASAPELDAKCHSTVVSGWFSEHQ
Sbjct: 1   MEADAGRGLGCQKTMDGKASNGIVPKKEIPSCCLKAMASAPELDAKCHSTVVSGWFSEHQ 60

Query: 61  SASDKGNKMVYFNNPMWPGEAHSLQVESILFQGRSEYQEVVVFESTSYGKVLVLDGIVQL 120
           SASDKGNKMVYFNNPMWPGEAHSLQVESILFQ RSEYQEVVVFESTSYGKVLVLDGIVQL
Sbjct: 61  SASDKGNKMVYFNNPMWPGEAHSLQVESILFQRRSEYQEVVVFESTSYGKVLVLDGIVQL 120

Query: 121 TEKDECAYQEMITHLPLCSIPSPKTVLVVGGGDGGVLREVARHNSVEHIDICEIDKMVID 180
           TEKDECAYQEMITHLPLCSIPSPKTVLVVGGGDGGVLREVARHNSVEHIDICEIDKMVID
Sbjct: 121 TEKDECAYQEMITHLPLCSIPSPKTVLVVGGGDGGVLREVARHNSVEHIDICEIDKMVID 180

Query: 181 VSKEFFPELAKGFEDPRVHLHVGDAVDFLRRAPKEKYDAIIVDSSDPVGPAQELVEKPFF 240
           VSKEFFPELAKGFEDPRVHLHVGDA+DFLRRAPKEKYDA+IVDSSDPVGPAQELVEKPFF
Sbjct: 181 VSKEFFPELAKGFEDPRVHLHVGDAIDFLRRAPKEKYDAVIVDSSDPVGPAQELVEKPFF 240

Query: 241 ETIAKALKPGGVLCNMAESMWLHTHLIQDMISICRKIFKGSVHYAWGSVPTYPSGVIGFL 300
           ETIAKALKPGGVLCNMAESMWLHTHLIQDMISICRKIFKGSVHYAWGSVPTYPSGVIGFL
Sbjct: 241 ETIAKALKPGGVLCNMAESMWLHTHLIQDMISICRKIFKGSVHYAWGSVPTYPSGVIGFL 300

Query: 301 LCSTEGPPVDFKNPINPIEKLESSAEYERELKFYNSEIHSAAFALPSFLRREVKALVDSP 360
           LCSTEGPPVDFKNPINPIEKLE SAEYERELKFYNSEIHSAAFALPSFLRREVKALVDSP
Sbjct: 301 LCSTEGPPVDFKNPINPIEKLEGSAEYERELKFYNSEIHSAAFALPSFLRREVKALVDSP 360

Query: 361 DPAQD 366
           DPAQD
Sbjct: 361 DPAQD 365

BLAST of Carg14021 vs. ExPASy TrEMBL
Match: A0A6J1H255 (spermine synthase-like isoform X1 OS=Cucurbita moschata OX=3662 GN=LOC111459343 PE=3 SV=1)

HSP 1 Score: 744.2 bits (1920), Expect = 2.6e-211
Identity = 364/367 (99.18%), Postives = 365/367 (99.46%), Query Frame = 0

Query: 1   MEADAGRGLGCQKTMDGKASNGIVPKKEIPSCCLKAMASAPELDAKCHSTVVSGWFSEHQ 60
           MEADAGRGLGCQKTMDGKASNGIVPKKEIPSCCLKAMASAPELDAKCHSTVVSGWFSEHQ
Sbjct: 1   MEADAGRGLGCQKTMDGKASNGIVPKKEIPSCCLKAMASAPELDAKCHSTVVSGWFSEHQ 60

Query: 61  SA--SDKGNKMVYFNNPMWPGEAHSLQVESILFQGRSEYQEVVVFESTSYGKVLVLDGIV 120
           SA  SDKGNKMVYFNNPMWPGEAHSLQVESILFQGRSEYQEVVVF+STSYGKVLVLDGIV
Sbjct: 61  SASDSDKGNKMVYFNNPMWPGEAHSLQVESILFQGRSEYQEVVVFKSTSYGKVLVLDGIV 120

Query: 121 QLTEKDECAYQEMITHLPLCSIPSPKTVLVVGGGDGGVLREVARHNSVEHIDICEIDKMV 180
           QLTEKDECAYQEMITHLPLCSIPSPKTVLVVGGGDGGVLREVARHNSVEHIDICEIDKMV
Sbjct: 121 QLTEKDECAYQEMITHLPLCSIPSPKTVLVVGGGDGGVLREVARHNSVEHIDICEIDKMV 180

Query: 181 IDVSKEFFPELAKGFEDPRVHLHVGDAVDFLRRAPKEKYDAIIVDSSDPVGPAQELVEKP 240
           IDVSKEFFPELAKGFEDPRVHLHVGDAVDFLRRAPKEKYDAIIVDSSDPVGPAQELVEKP
Sbjct: 181 IDVSKEFFPELAKGFEDPRVHLHVGDAVDFLRRAPKEKYDAIIVDSSDPVGPAQELVEKP 240

Query: 241 FFETIAKALKPGGVLCNMAESMWLHTHLIQDMISICRKIFKGSVHYAWGSVPTYPSGVIG 300
           FFETIAKALKPGGVLCNMAESMWLHTHLIQDMISICRKIFKGSVHYAWGSVPTYPSGVIG
Sbjct: 241 FFETIAKALKPGGVLCNMAESMWLHTHLIQDMISICRKIFKGSVHYAWGSVPTYPSGVIG 300

Query: 301 FLLCSTEGPPVDFKNPINPIEKLESSAEYERELKFYNSEIHSAAFALPSFLRREVKALVD 360
           FLLCSTEGPPVDFKNPINPIEKLESSAEYERELKFYNSEIHSAAFALPSFLRREVKALVD
Sbjct: 301 FLLCSTEGPPVDFKNPINPIEKLESSAEYERELKFYNSEIHSAAFALPSFLRREVKALVD 360

Query: 361 SPDPAQD 366
           SPDPAQD
Sbjct: 361 SPDPAQD 367

BLAST of Carg14021 vs. ExPASy TrEMBL
Match: A0A6J1K307 (spermine synthase-like isoform X1 OS=Cucurbita maxima OX=3661 GN=LOC111491249 PE=3 SV=1)

HSP 1 Score: 740.3 bits (1910), Expect = 3.8e-210
Identity = 361/367 (98.37%), Postives = 363/367 (98.91%), Query Frame = 0

Query: 1   MEADAGRGLGCQKTMDGKASNGIVPKKEIPSCCLKAMASAPELDAKCHSTVVSGWFSEHQ 60
           MEADAGRGLGCQKTMDGKASNGIVPKKEIPSCCLKAMASAPELDAKCHSTVVSGWFSEHQ
Sbjct: 1   MEADAGRGLGCQKTMDGKASNGIVPKKEIPSCCLKAMASAPELDAKCHSTVVSGWFSEHQ 60

Query: 61  SA--SDKGNKMVYFNNPMWPGEAHSLQVESILFQGRSEYQEVVVFESTSYGKVLVLDGIV 120
           SA  SDKGNKMVYFNNPMWPGEAHSLQVESILFQ RSEYQEVVVFESTSYGKVLVLDGIV
Sbjct: 61  SASDSDKGNKMVYFNNPMWPGEAHSLQVESILFQRRSEYQEVVVFESTSYGKVLVLDGIV 120

Query: 121 QLTEKDECAYQEMITHLPLCSIPSPKTVLVVGGGDGGVLREVARHNSVEHIDICEIDKMV 180
           QLTEKDECAYQEMITHLPLCSIPSPKTVLVVGGGDGGVLREVARHNSVEHIDICEIDKMV
Sbjct: 121 QLTEKDECAYQEMITHLPLCSIPSPKTVLVVGGGDGGVLREVARHNSVEHIDICEIDKMV 180

Query: 181 IDVSKEFFPELAKGFEDPRVHLHVGDAVDFLRRAPKEKYDAIIVDSSDPVGPAQELVEKP 240
           IDVSKEFFPELAKGFEDPRVHLHVGDA+DFLRRAPKEKYDA+IVDSSDPVGPAQELVEKP
Sbjct: 181 IDVSKEFFPELAKGFEDPRVHLHVGDAIDFLRRAPKEKYDAVIVDSSDPVGPAQELVEKP 240

Query: 241 FFETIAKALKPGGVLCNMAESMWLHTHLIQDMISICRKIFKGSVHYAWGSVPTYPSGVIG 300
           FFETIAKALKPGGVLCNMAESMWLHTHLIQDMISICRKIFKGSVHYAWGSVPTYPSGVIG
Sbjct: 241 FFETIAKALKPGGVLCNMAESMWLHTHLIQDMISICRKIFKGSVHYAWGSVPTYPSGVIG 300

Query: 301 FLLCSTEGPPVDFKNPINPIEKLESSAEYERELKFYNSEIHSAAFALPSFLRREVKALVD 360
           FLLCSTEGPPVDFKNPINPIEKLE SAEYERELKFYNSEIHSAAFALPSFLRREVKALVD
Sbjct: 301 FLLCSTEGPPVDFKNPINPIEKLEGSAEYERELKFYNSEIHSAAFALPSFLRREVKALVD 360

Query: 361 SPDPAQD 366
           SPDPAQD
Sbjct: 361 SPDPAQD 367

BLAST of Carg14021 vs. ExPASy TrEMBL
Match: A0A0A0KN88 (PABS domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_5G139500 PE=3 SV=1)

HSP 1 Score: 677.6 bits (1747), Expect = 3.0e-191
Identity = 320/365 (87.67%), Postives = 349/365 (95.62%), Query Frame = 0

Query: 1   MEADAGRGLGCQKTMDGKASNGIVPKKEIPSCCLKAMASAPELDAKCHSTVVSGWFSEHQ 60
           MEADAGRGLGCQKTMDG+ASNGI+ KKEIPSCCLKA AS PEL+AKCHSTVVSGWFSE Q
Sbjct: 1   MEADAGRGLGCQKTMDGRASNGILTKKEIPSCCLKARASVPELEAKCHSTVVSGWFSEPQ 60

Query: 61  SASDKGNKMVYFNNPMWPGEAHSLQVESILFQGRSEYQEVVVFESTSYGKVLVLDGIVQL 120
            A D G K VYFNNPMWPGEAHSLQVESILF+G+SE+QEVVVFEST+YGKVLVLDGIVQL
Sbjct: 61  FAYDDGKKRVYFNNPMWPGEAHSLQVESILFKGKSEFQEVVVFESTTYGKVLVLDGIVQL 120

Query: 121 TEKDECAYQEMITHLPLCSIPSPKTVLVVGGGDGGVLREVARHNSVEHIDICEIDKMVID 180
           TEKDECAYQEMITHLPLCSIPSPKTVLVVGGGDGGVL E++RHNS+EHIDICEID+MVID
Sbjct: 121 TEKDECAYQEMITHLPLCSIPSPKTVLVVGGGDGGVLSEISRHNSIEHIDICEIDQMVID 180

Query: 181 VSKEFFPELAKGFEDPRVHLHVGDAVDFLRRAPKEKYDAIIVDSSDPVGPAQELVEKPFF 240
           VSKEFFP+LA GFEDPRVHLHVGDAV+FLRRAP+ KYDAIIVDSSDPVGPAQELVEKPFF
Sbjct: 181 VSKEFFPDLAIGFEDPRVHLHVGDAVEFLRRAPRGKYDAIIVDSSDPVGPAQELVEKPFF 240

Query: 241 ETIAKALKPGGVLCNMAESMWLHTHLIQDMISICRKIFKGSVHYAWGSVPTYPSGVIGFL 300
           ET+AKALKPGGVLCNMAESMWLHTHLI DMISICR++FKGS+HYAW SVPTYPSGVIGFL
Sbjct: 241 ETLAKALKPGGVLCNMAESMWLHTHLIDDMISICREVFKGSIHYAWASVPTYPSGVIGFL 300

Query: 301 LCSTEGPPVDFKNPINPIEKLESSAEYERELKFYNSEIHSAAFALPSFLRREVKALVDSP 360
           LCSTEGPPVDFKNPINPIEKLE + +++++LKFYNSE+HSAAFALPSFLR+EVKALVDSP
Sbjct: 301 LCSTEGPPVDFKNPINPIEKLEGAVKHKKDLKFYNSEMHSAAFALPSFLRKEVKALVDSP 360

Query: 361 DPAQD 366
           +P+Q+
Sbjct: 361 NPSQN 365

BLAST of Carg14021 vs. TAIR 10
Match: AT5G53120.1 (spermidine synthase 3 )

HSP 1 Score: 588.6 bits (1516), Expect = 3.5e-168
Identity = 283/360 (78.61%), Postives = 318/360 (88.33%), Query Frame = 0

Query: 1   MEADAGRGLGCQKTMDGKASNGIVPKKEIPSCCLKAMASAPELDAKCHSTVVSGWFSE-H 60
           ME D G GL CQ TMDGKASNG   +K +PSCCLKAMA  PE DAKCHSTVVSGWFSE H
Sbjct: 1   MEGDVGIGLVCQNTMDGKASNGNGLEKTVPSCCLKAMACVPEDDAKCHSTVVSGWFSEPH 60

Query: 61  QSASDKGNKMVYFNNPMWPGEAHSLQVESILFQGRSEYQEVVVFESTSYGKVLVLDGIVQ 120
             +  KG K VYFNNPMWPGEAHSL+VE +LF+ +S++QEV+VFES +YGKVLVLDGIVQ
Sbjct: 61  PRSGKKGGKAVYFNNPMWPGEAHSLKVEKVLFKDKSDFQEVLVFESATYGKVLVLDGIVQ 120

Query: 121 LTEKDECAYQEMITHLPLCSIPSPKTVLVVGGGDGGVLREVARHNSVEHIDICEIDKMVI 180
           LTEKDECAYQEMI HLPLCSI SPK VLVVGGGDGGVLRE++RH+SVE IDICEIDKMVI
Sbjct: 121 LTEKDECAYQEMIAHLPLCSISSPKNVLVVGGGDGGVLREISRHSSVEVIDICEIDKMVI 180

Query: 181 DVSKEFFPELAKGFEDPRVHLHVGDAVDFLRRAPKEKYDAIIVDSSDPVGPAQELVEKPF 240
           DVSK+FFPELA GF+DPRV LH+GDA +FLR++P+ KYDAIIVDSSDPVGPA  LVEKPF
Sbjct: 181 DVSKKFFPELAVGFDDPRVQLHIGDAAEFLRKSPEGKYDAIIVDSSDPVGPALALVEKPF 240

Query: 241 FETIAKALKPGGVLCNMAESMWLHTHLIQDMISICRKIFKGSVHYAWGSVPTYPSGVIGF 300
           FET+A+ALKPGGVLCNMAESMWLHTHLI+DMISICR+ FK SVHYAW SVPTYPSGVIGF
Sbjct: 241 FETLARALKPGGVLCNMAESMWLHTHLIEDMISICRQTFK-SVHYAWSSVPTYPSGVIGF 300

Query: 301 LLCSTEGPPVDFKNPINPIEKLESSAEYERELKFYNSEIHSAAFALPSFLRREVKALVDS 360
           +LCSTEGP VDFKNPINPIEKL+ +  ++RELKFYNS++H AAFALP+FLRREV +L+ S
Sbjct: 301 VLCSTEGPAVDFKNPINPIEKLDGAMTHKRELKFYNSDMHRAAFALPTFLRREVASLLAS 359

BLAST of Carg14021 vs. TAIR 10
Match: AT5G53120.2 (spermidine synthase 3 )

HSP 1 Score: 588.6 bits (1516), Expect = 3.5e-168
Identity = 283/360 (78.61%), Postives = 318/360 (88.33%), Query Frame = 0

Query: 1   MEADAGRGLGCQKTMDGKASNGIVPKKEIPSCCLKAMASAPELDAKCHSTVVSGWFSE-H 60
           ME D G GL CQ TMDGKASNG   +K +PSCCLKAMA  PE DAKCHSTVVSGWFSE H
Sbjct: 1   MEGDVGIGLVCQNTMDGKASNGNGLEKTVPSCCLKAMACVPEDDAKCHSTVVSGWFSEPH 60

Query: 61  QSASDKGNKMVYFNNPMWPGEAHSLQVESILFQGRSEYQEVVVFESTSYGKVLVLDGIVQ 120
             +  KG K VYFNNPMWPGEAHSL+VE +LF+ +S++QEV+VFES +YGKVLVLDGIVQ
Sbjct: 61  PRSGKKGGKAVYFNNPMWPGEAHSLKVEKVLFKDKSDFQEVLVFESATYGKVLVLDGIVQ 120

Query: 121 LTEKDECAYQEMITHLPLCSIPSPKTVLVVGGGDGGVLREVARHNSVEHIDICEIDKMVI 180
           LTEKDECAYQEMI HLPLCSI SPK VLVVGGGDGGVLRE++RH+SVE IDICEIDKMVI
Sbjct: 121 LTEKDECAYQEMIAHLPLCSISSPKNVLVVGGGDGGVLREISRHSSVEVIDICEIDKMVI 180

Query: 181 DVSKEFFPELAKGFEDPRVHLHVGDAVDFLRRAPKEKYDAIIVDSSDPVGPAQELVEKPF 240
           DVSK+FFPELA GF+DPRV LH+GDA +FLR++P+ KYDAIIVDSSDPVGPA  LVEKPF
Sbjct: 181 DVSKKFFPELAVGFDDPRVQLHIGDAAEFLRKSPEGKYDAIIVDSSDPVGPALALVEKPF 240

Query: 241 FETIAKALKPGGVLCNMAESMWLHTHLIQDMISICRKIFKGSVHYAWGSVPTYPSGVIGF 300
           FET+A+ALKPGGVLCNMAESMWLHTHLI+DMISICR+ FK SVHYAW SVPTYPSGVIGF
Sbjct: 241 FETLARALKPGGVLCNMAESMWLHTHLIEDMISICRQTFK-SVHYAWSSVPTYPSGVIGF 300

Query: 301 LLCSTEGPPVDFKNPINPIEKLESSAEYERELKFYNSEIHSAAFALPSFLRREVKALVDS 360
           +LCSTEGP VDFKNPINPIEKL+ +  ++RELKFYNS++H AAFALP+FLRREV +L+ S
Sbjct: 301 VLCSTEGPAVDFKNPINPIEKLDGAMTHKRELKFYNSDMHRAAFALPTFLRREVASLLAS 359

BLAST of Carg14021 vs. TAIR 10
Match: AT5G53120.3 (spermidine synthase 3 )

HSP 1 Score: 588.6 bits (1516), Expect = 3.5e-168
Identity = 283/360 (78.61%), Postives = 318/360 (88.33%), Query Frame = 0

Query: 1   MEADAGRGLGCQKTMDGKASNGIVPKKEIPSCCLKAMASAPELDAKCHSTVVSGWFSE-H 60
           ME D G GL CQ TMDGKASNG   +K +PSCCLKAMA  PE DAKCHSTVVSGWFSE H
Sbjct: 1   MEGDVGIGLVCQNTMDGKASNGNGLEKTVPSCCLKAMACVPEDDAKCHSTVVSGWFSEPH 60

Query: 61  QSASDKGNKMVYFNNPMWPGEAHSLQVESILFQGRSEYQEVVVFESTSYGKVLVLDGIVQ 120
             +  KG K VYFNNPMWPGEAHSL+VE +LF+ +S++QEV+VFES +YGKVLVLDGIVQ
Sbjct: 61  PRSGKKGGKAVYFNNPMWPGEAHSLKVEKVLFKDKSDFQEVLVFESATYGKVLVLDGIVQ 120

Query: 121 LTEKDECAYQEMITHLPLCSIPSPKTVLVVGGGDGGVLREVARHNSVEHIDICEIDKMVI 180
           LTEKDECAYQEMI HLPLCSI SPK VLVVGGGDGGVLRE++RH+SVE IDICEIDKMVI
Sbjct: 121 LTEKDECAYQEMIAHLPLCSISSPKNVLVVGGGDGGVLREISRHSSVEVIDICEIDKMVI 180

Query: 181 DVSKEFFPELAKGFEDPRVHLHVGDAVDFLRRAPKEKYDAIIVDSSDPVGPAQELVEKPF 240
           DVSK+FFPELA GF+DPRV LH+GDA +FLR++P+ KYDAIIVDSSDPVGPA  LVEKPF
Sbjct: 181 DVSKKFFPELAVGFDDPRVQLHIGDAAEFLRKSPEGKYDAIIVDSSDPVGPALALVEKPF 240

Query: 241 FETIAKALKPGGVLCNMAESMWLHTHLIQDMISICRKIFKGSVHYAWGSVPTYPSGVIGF 300
           FET+A+ALKPGGVLCNMAESMWLHTHLI+DMISICR+ FK SVHYAW SVPTYPSGVIGF
Sbjct: 241 FETLARALKPGGVLCNMAESMWLHTHLIEDMISICRQTFK-SVHYAWSSVPTYPSGVIGF 300

Query: 301 LLCSTEGPPVDFKNPINPIEKLESSAEYERELKFYNSEIHSAAFALPSFLRREVKALVDS 360
           +LCSTEGP VDFKNPINPIEKL+ +  ++RELKFYNS++H AAFALP+FLRREV +L+ S
Sbjct: 301 VLCSTEGPAVDFKNPINPIEKLDGAMTHKRELKFYNSDMHRAAFALPTFLRREVASLLAS 359

BLAST of Carg14021 vs. TAIR 10
Match: AT5G53120.4 (spermidine synthase 3 )

HSP 1 Score: 588.6 bits (1516), Expect = 3.5e-168
Identity = 283/360 (78.61%), Postives = 318/360 (88.33%), Query Frame = 0

Query: 1   MEADAGRGLGCQKTMDGKASNGIVPKKEIPSCCLKAMASAPELDAKCHSTVVSGWFSE-H 60
           ME D G GL CQ TMDGKASNG   +K +PSCCLKAMA  PE DAKCHSTVVSGWFSE H
Sbjct: 1   MEGDVGIGLVCQNTMDGKASNGNGLEKTVPSCCLKAMACVPEDDAKCHSTVVSGWFSEPH 60

Query: 61  QSASDKGNKMVYFNNPMWPGEAHSLQVESILFQGRSEYQEVVVFESTSYGKVLVLDGIVQ 120
             +  KG K VYFNNPMWPGEAHSL+VE +LF+ +S++QEV+VFES +YGKVLVLDGIVQ
Sbjct: 61  PRSGKKGGKAVYFNNPMWPGEAHSLKVEKVLFKDKSDFQEVLVFESATYGKVLVLDGIVQ 120

Query: 121 LTEKDECAYQEMITHLPLCSIPSPKTVLVVGGGDGGVLREVARHNSVEHIDICEIDKMVI 180
           LTEKDECAYQEMI HLPLCSI SPK VLVVGGGDGGVLRE++RH+SVE IDICEIDKMVI
Sbjct: 121 LTEKDECAYQEMIAHLPLCSISSPKNVLVVGGGDGGVLREISRHSSVEVIDICEIDKMVI 180

Query: 181 DVSKEFFPELAKGFEDPRVHLHVGDAVDFLRRAPKEKYDAIIVDSSDPVGPAQELVEKPF 240
           DVSK+FFPELA GF+DPRV LH+GDA +FLR++P+ KYDAIIVDSSDPVGPA  LVEKPF
Sbjct: 181 DVSKKFFPELAVGFDDPRVQLHIGDAAEFLRKSPEGKYDAIIVDSSDPVGPALALVEKPF 240

Query: 241 FETIAKALKPGGVLCNMAESMWLHTHLIQDMISICRKIFKGSVHYAWGSVPTYPSGVIGF 300
           FET+A+ALKPGGVLCNMAESMWLHTHLI+DMISICR+ FK SVHYAW SVPTYPSGVIGF
Sbjct: 241 FETLARALKPGGVLCNMAESMWLHTHLIEDMISICRQTFK-SVHYAWSSVPTYPSGVIGF 300

Query: 301 LLCSTEGPPVDFKNPINPIEKLESSAEYERELKFYNSEIHSAAFALPSFLRREVKALVDS 360
           +LCSTEGP VDFKNPINPIEKL+ +  ++RELKFYNS++H AAFALP+FLRREV +L+ S
Sbjct: 301 VLCSTEGPAVDFKNPINPIEKLDGAMTHKRELKFYNSDMHRAAFALPTFLRREVASLLAS 359

BLAST of Carg14021 vs. TAIR 10
Match: AT5G53120.5 (spermidine synthase 3 )

HSP 1 Score: 588.6 bits (1516), Expect = 3.5e-168
Identity = 283/360 (78.61%), Postives = 318/360 (88.33%), Query Frame = 0

Query: 1   MEADAGRGLGCQKTMDGKASNGIVPKKEIPSCCLKAMASAPELDAKCHSTVVSGWFSE-H 60
           ME D G GL CQ TMDGKASNG   +K +PSCCLKAMA  PE DAKCHSTVVSGWFSE H
Sbjct: 1   MEGDVGIGLVCQNTMDGKASNGNGLEKTVPSCCLKAMACVPEDDAKCHSTVVSGWFSEPH 60

Query: 61  QSASDKGNKMVYFNNPMWPGEAHSLQVESILFQGRSEYQEVVVFESTSYGKVLVLDGIVQ 120
             +  KG K VYFNNPMWPGEAHSL+VE +LF+ +S++QEV+VFES +YGKVLVLDGIVQ
Sbjct: 61  PRSGKKGGKAVYFNNPMWPGEAHSLKVEKVLFKDKSDFQEVLVFESATYGKVLVLDGIVQ 120

Query: 121 LTEKDECAYQEMITHLPLCSIPSPKTVLVVGGGDGGVLREVARHNSVEHIDICEIDKMVI 180
           LTEKDECAYQEMI HLPLCSI SPK VLVVGGGDGGVLRE++RH+SVE IDICEIDKMVI
Sbjct: 121 LTEKDECAYQEMIAHLPLCSISSPKNVLVVGGGDGGVLREISRHSSVEVIDICEIDKMVI 180

Query: 181 DVSKEFFPELAKGFEDPRVHLHVGDAVDFLRRAPKEKYDAIIVDSSDPVGPAQELVEKPF 240
           DVSK+FFPELA GF+DPRV LH+GDA +FLR++P+ KYDAIIVDSSDPVGPA  LVEKPF
Sbjct: 181 DVSKKFFPELAVGFDDPRVQLHIGDAAEFLRKSPEGKYDAIIVDSSDPVGPALALVEKPF 240

Query: 241 FETIAKALKPGGVLCNMAESMWLHTHLIQDMISICRKIFKGSVHYAWGSVPTYPSGVIGF 300
           FET+A+ALKPGGVLCNMAESMWLHTHLI+DMISICR+ FK SVHYAW SVPTYPSGVIGF
Sbjct: 241 FETLARALKPGGVLCNMAESMWLHTHLIEDMISICRQTFK-SVHYAWSSVPTYPSGVIGF 300

Query: 301 LLCSTEGPPVDFKNPINPIEKLESSAEYERELKFYNSEIHSAAFALPSFLRREVKALVDS 360
           +LCSTEGP VDFKNPINPIEKL+ +  ++RELKFYNS++H AAFALP+FLRREV +L+ S
Sbjct: 301 VLCSTEGPAVDFKNPINPIEKLDGAMTHKRELKFYNSDMHRAAFALPTFLRREVASLLAS 359

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
KAG7035744.15.7e-213100.00Spermine synthase [Cucurbita argyrosperma subsp. argyrosperma][more]
XP_022957976.11.7e-21299.73spermine synthase-like isoform X2 [Cucurbita moschata][more]
XP_022995840.12.4e-21198.90spermine synthase-like isoform X2 [Cucurbita maxima] >XP_022995841.1 spermine sy... [more]
XP_022957972.15.4e-21199.18spermine synthase-like isoform X1 [Cucurbita moschata] >XP_022957974.1 spermine ... [more]
XP_022995836.17.8e-21098.37spermine synthase-like isoform X1 [Cucurbita maxima] >XP_022995837.1 spermine sy... [more]
Match NameE-valueIdentityDescription
Q94BN24.9e-16778.61Spermine synthase OS=Arabidopsis thaliana OX=3702 GN=SPMS PE=1 SV=1[more]
O821473.1e-12970.16Spermidine synthase OS=Coffea arabica OX=13443 PE=2 SV=1[more]
O486592.7e-12869.38Spermidine synthase 2 OS=Hyoscyamus niger OX=4079 PE=2 SV=1[more]
Q965561.1e-12665.83Spermidine synthase 1 OS=Datura stramonium OX=4076 PE=2 SV=1[more]
Q9ZUB31.5e-12667.31Spermidine synthase 1 OS=Arabidopsis thaliana OX=3702 GN=SPDSYN1 PE=1 SV=1[more]
Match NameE-valueIdentityDescription
A0A6J1H0Q58.1e-21399.73spermine synthase-like isoform X2 OS=Cucurbita moschata OX=3662 GN=LOC111459343 ... [more]
A0A6J1K5161.2e-21198.90spermine synthase-like isoform X2 OS=Cucurbita maxima OX=3661 GN=LOC111491249 PE... [more]
A0A6J1H2552.6e-21199.18spermine synthase-like isoform X1 OS=Cucurbita moschata OX=3662 GN=LOC111459343 ... [more]
A0A6J1K3073.8e-21098.37spermine synthase-like isoform X1 OS=Cucurbita maxima OX=3661 GN=LOC111491249 PE... [more]
A0A0A0KN883.0e-19187.67PABS domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_5G139500 PE=3 S... [more]
Match NameE-valueIdentityDescription
AT5G53120.13.5e-16878.61spermidine synthase 3 [more]
AT5G53120.23.5e-16878.61spermidine synthase 3 [more]
AT5G53120.33.5e-16878.61spermidine synthase 3 [more]
AT5G53120.43.5e-16878.61spermidine synthase 3 [more]
AT5G53120.53.5e-16878.61spermidine synthase 3 [more]
InterPro
Analysis Name: InterPro Annotations of Silver-seed gourd (SMH-JMG-627) v2
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR001045Spermidine/spermine synthasesTIGRFAMTIGR00417TIGR00417coord: 79..348
e-value: 3.8E-93
score: 309.6
IPR001045Spermidine/spermine synthasesPANTHERPTHR11558SPERMIDINE/SPERMINE SYNTHASEcoord: 1..361
IPR001045Spermidine/spermine synthasesHAMAPMF_00198Spermidine_synthcoord: 54..354
score: 35.303967
NoneNo IPR availablePFAMPF01564Spermine_synthcoord: 125..312
e-value: 1.2E-65
score: 220.5
NoneNo IPR availableGENE3D3.40.50.150Vaccinia Virus protein VP39coord: 120..357
e-value: 3.6E-98
score: 329.7
NoneNo IPR availablePANTHERPTHR11558:SF25SPERMINE SYNTHASEcoord: 1..361
NoneNo IPR availableCDDcd02440AdoMet_MTasescoord: 158..253
e-value: 5.01036E-10
score: 54.3583
IPR035246Spermidine synthase, tetramerisation domainPFAMPF17284Spermine_synt_Ncoord: 75..122
e-value: 1.5E-19
score: 69.6
IPR037163Spermidine synthase, tetramerisation domain superfamilyGENE3D2.30.140.10Spermidine synthase, tetramerisation domaincoord: 46..119
e-value: 1.2E-28
score: 101.3
IPR030668Spermidine/spermine synthase, eukaryotesPIRSFPIRSF000502Spermidine_synthcoord: 42..358
e-value: 5.9E-139
score: 459.9
IPR030373Polyamine biosynthesis domain, conserved sitePROSITEPS01330PABS_1coord: 146..159
IPR030374Polyamine biosynthesis domainPROSITEPS51006PABS_2coord: 53..304
score: 99.506294
IPR029063S-adenosyl-L-methionine-dependent methyltransferaseSUPERFAMILY53335S-adenosyl-L-methionine-dependent methyltransferasescoord: 70..353

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Carg14021-RACarg14021-RAmRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0006596 polyamine biosynthetic process
biological_process GO:0006595 polyamine metabolic process
molecular_function GO:0004766 spermidine synthase activity
molecular_function GO:0003824 catalytic activity