Carg14015 (gene) Silver-seed gourd (SMH-JMG-627) v2

BLAST of Carg14015 vs. NCBI nr
Match: KAG7035750.1 (putative pectinesterase/pectinesterase inhibitor 61, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 731.9 bits (1888), Expect = 2.9e-207
Identity = 382/382 (100.00%), Postives = 382/382 (100.00%), Query Frame = 0

Query: 1   MNNYNKLHSSASGDTPATFQVSHTPNPPSSSASRRRTRILFVSLISILLITASAVSAAVL 60
           MNNYNKLHSSASGDTPATFQVSHTPNPPSSSASRRRTRILFVSLISILLITASAVSAAVL
Sbjct: 1   MNNYNKLHSSASGDTPATFQVSHTPNPPSSSASRRRTRILFVSLISILLITASAVSAAVL 60

Query: 61  IHSHTSHFPHNKPTQAISHACSHTLYPSLCRSSLLSFPGSLSADVHRLVHISLNLTLQHL 120
           IHSHTSHFPHNKPTQAISHACSHTLYPSLCRSSLLSFPGSLSADVHRLVHISLNLTLQHL
Sbjct: 61  IHSHTSHFPHNKPTQAISHACSHTLYPSLCRSSLLSFPGSLSADVHRLVHISLNLTLQHL 120

Query: 121 TKALYASSQIPVFQISNNPLAHSAYQDCIELLNDAVDAFSLSLFSTSASNHDIMTWLSAA 180
           TKALYASSQIPVFQISNNPLAHSAYQDCIELLNDAVDAFSLSLFSTSASNHDIMTWLSAA
Sbjct: 121 TKALYASSQIPVFQISNNPLAHSAYQDCIELLNDAVDAFSLSLFSTSASNHDIMTWLSAA 180

Query: 181 LTYHDTCSDGFADVDDPVKDQMAARLSDLSEMISNSLAIFSGYGEELPVQNRRRLMESDR 240
           LTYHDTCSDGFADVDDPVKDQMAARLSDLSEMISNSLAIFSGYGEELPVQNRRRLMESDR
Sbjct: 181 LTYHDTCSDGFADVDDPVKDQMAARLSDLSEMISNSLAIFSGYGEELPVQNRRRLMESDR 240

Query: 241 EWVAENGVAVAEGEFPGWVSGKDRRLLAAPISAIQADIVVAKDGSGKFTTVAEAIEAAPS 300
           EWVAENGVAVAEGEFPGWVSGKDRRLLAAPISAIQADIVVAKDGSGKFTTVAEAIEAAPS
Sbjct: 241 EWVAENGVAVAEGEFPGWVSGKDRRLLAAPISAIQADIVVAKDGSGKFTTVAEAIEAAPS 300

Query: 301 SSGRRIVIYIKAGKYEEENLKVGRKKTNLMFVGDGKGITVISGGKSVFDKVTTFRTATFG 360
           SSGRRIVIYIKAGKYEEENLKVGRKKTNLMFVGDGKGITVISGGKSVFDKVTTFRTATFG
Sbjct: 301 SSGRRIVIYIKAGKYEEENLKVGRKKTNLMFVGDGKGITVISGGKSVFDKVTTFRTATFG 360

Query: 361 IYSPSLFSILFQNFFLFLILMN 383
           IYSPSLFSILFQNFFLFLILMN
Sbjct: 361 IYSPSLFSILFQNFFLFLILMN 382

BLAST of Carg14015 vs. NCBI nr
Match: KAG6605784.1 (putative pectinesterase/pectinesterase inhibitor 61, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 688.3 bits (1775), Expect = 3.7e-194
Identity = 357/359 (99.44%), Postives = 357/359 (99.44%), Query Frame = 0

Query: 1   MNNYNKLHSSASGDTPATFQVSHTPNPPSSSASRRRTRILFVSLISILLITASAVSAAVL 60
           MNNYNKLHSSASGDTPATFQVSHTPNPPSSSASRRRTRILFVSLISILLI  SAVSAAVL
Sbjct: 1   MNNYNKLHSSASGDTPATFQVSHTPNPPSSSASRRRTRILFVSLISILLIAVSAVSAAVL 60

Query: 61  IHSHTSHFPHNKPTQAISHACSHTLYPSLCRSSLLSFPGSLSADVHRLVHISLNLTLQHL 120
           IHSHTSHFPHNKPTQAISHACSHTLYPSLCRSSLLSFPGSLSADVHRLVHISLNLTLQHL
Sbjct: 61  IHSHTSHFPHNKPTQAISHACSHTLYPSLCRSSLLSFPGSLSADVHRLVHISLNLTLQHL 120

Query: 121 TKALYASSQIPVFQISNNPLAHSAYQDCIELLNDAVDAFSLSLFSTSASNHDIMTWLSAA 180
           TKALYASSQIPVFQISNNPLAHSAYQDCIELLNDAVDAFSLSLFSTSASNHDIMTWLSAA
Sbjct: 121 TKALYASSQIPVFQISNNPLAHSAYQDCIELLNDAVDAFSLSLFSTSASNHDIMTWLSAA 180

Query: 181 LTYHDTCSDGFADVDDPVKDQMAARLSDLSEMISNSLAIFSGYGEELPVQNRRRLMESDR 240
           LTYHDTCSDGFADVDDPVKDQMAARLSDLSEMISNSLAIFSGYGEELPVQNRRRLMESDR
Sbjct: 181 LTYHDTCSDGFADVDDPVKDQMAARLSDLSEMISNSLAIFSGYGEELPVQNRRRLMESDR 240

Query: 241 EWVAENGVAVAEGEFPGWVSGKDRRLLAAPISAIQADIVVAKDGSGKFTTVAEAIEAAPS 300
           EWVAENGVAVAEGEFPGWVSGKDRRLLAAPISAIQADIVVAKDGSGKFTTVAEAIEAAPS
Sbjct: 241 EWVAENGVAVAEGEFPGWVSGKDRRLLAAPISAIQADIVVAKDGSGKFTTVAEAIEAAPS 300

Query: 301 SSGRRIVIYIKAGKYEEENLKVGRKKTNLMFVGDGKGITVISGGKSVFDKVTTFRTATF 360
           SSGRRIVIYIKAGKYEEENLKVGRKKTNLMFVGDGKGITVISGGKSVFDKVTTFRTATF
Sbjct: 301 SSGRRIVIYIKAGKYEEENLKVGRKKTNLMFVGDGKGITVISGGKSVFDKVTTFRTATF 359

BLAST of Carg14015 vs. NCBI nr
Match: XP_022957644.1 (probable pectinesterase/pectinesterase inhibitor 61 [Cucurbita moschata])

HSP 1 Score: 679.9 bits (1753), Expect = 1.3e-191
Identity = 353/359 (98.33%), Postives = 355/359 (98.89%), Query Frame = 0

Query: 1   MNNYNKLHSSASGDTPATFQVSHTPNPPSSSASRRRTRILFVSLISILLITASAVSAAVL 60
           MNNYNKLHSSASGDTPATFQVSHTPNPPSSSASRRRTRILFVSLISILLITASAVSAAVL
Sbjct: 1   MNNYNKLHSSASGDTPATFQVSHTPNPPSSSASRRRTRILFVSLISILLITASAVSAAVL 60

Query: 61  IHSHTSHFPHNKPTQAISHACSHTLYPSLCRSSLLSFPGSLSADVHRLVHISLNLTLQHL 120
           +HS +SHFPHNKPTQAISHACSHTLYPSLCRSSLLSFPGSLSADVHRLVHISLNLTLQHL
Sbjct: 61  LHSQSSHFPHNKPTQAISHACSHTLYPSLCRSSLLSFPGSLSADVHRLVHISLNLTLQHL 120

Query: 121 TKALYASSQIPVFQISNNPLAHSAYQDCIELLNDAVDAFSLSLFSTSASNHDIMTWLSAA 180
           TKALYASSQIPVFQISNNPLAHSAYQDCIELLNDAVDAFSLSLFSTSASNHDIMTWLSAA
Sbjct: 121 TKALYASSQIPVFQISNNPLAHSAYQDCIELLNDAVDAFSLSLFSTSASNHDIMTWLSAA 180

Query: 181 LTYHDTCSDGFADVDDPVKDQMAARLSDLSEMISNSLAIFSGYGEELPVQNRRRLMESDR 240
           LTYHDTCSDGFADVDDPVKDQMAARLSDLSEMISNSLAIFSGYGE LPVQNRRRLMESDR
Sbjct: 181 LTYHDTCSDGFADVDDPVKDQMAARLSDLSEMISNSLAIFSGYGEGLPVQNRRRLMESDR 240

Query: 241 EWVAENGVAVAEGEFPGWVSGKDRRLLAAPISAIQADIVVAKDGSGKFTTVAEAIEAAPS 300
           EW AENGVAVAEGEFPGWVSGKDRRLLAAPISAIQADIVVAKDGSGKF TVAEAIEAAPS
Sbjct: 241 EWAAENGVAVAEGEFPGWVSGKDRRLLAAPISAIQADIVVAKDGSGKFKTVAEAIEAAPS 300

Query: 301 SSGRRIVIYIKAGKYEEENLKVGRKKTNLMFVGDGKGITVISGGKSVFDKVTTFRTATF 360
           SSGRRIVIYIKAGKYEEENLKVGRKKTNLMFVGDGKGITVISGGKSVFDKVTTFRTATF
Sbjct: 301 SSGRRIVIYIKAGKYEEENLKVGRKKTNLMFVGDGKGITVISGGKSVFDKVTTFRTATF 359

BLAST of Carg14015 vs. NCBI nr
Match: XP_023533011.1 (probable pectinesterase/pectinesterase inhibitor 61 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 676.8 bits (1745), Expect = 1.1e-190
Identity = 351/359 (97.77%), Postives = 354/359 (98.61%), Query Frame = 0

Query: 1   MNNYNKLHSSASGDTPATFQVSHTPNPPSSSASRRRTRILFVSLISILLITASAVSAAVL 60
           MNNYNKLHSS SGDTPATFQVSHTPNPPSSSASRRRTRILFVSLISILLITASAVSAAVL
Sbjct: 1   MNNYNKLHSSGSGDTPATFQVSHTPNPPSSSASRRRTRILFVSLISILLITASAVSAAVL 60

Query: 61  IHSHTSHFPHNKPTQAISHACSHTLYPSLCRSSLLSFPGSLSADVHRLVHISLNLTLQHL 120
           IH++TSHFPHNKPTQAISHACSHTLYPSLCRSSLLSFPGSLSADVH LVHISLNLTLQHL
Sbjct: 61  IHTNTSHFPHNKPTQAISHACSHTLYPSLCRSSLLSFPGSLSADVHHLVHISLNLTLQHL 120

Query: 121 TKALYASSQIPVFQISNNPLAHSAYQDCIELLNDAVDAFSLSLFSTSASNHDIMTWLSAA 180
           TKALYASSQIPVFQISNNPLAHSAYQDCIELLNDAVDAFSLSLFSTSASNHDIMTWLSAA
Sbjct: 121 TKALYASSQIPVFQISNNPLAHSAYQDCIELLNDAVDAFSLSLFSTSASNHDIMTWLSAA 180

Query: 181 LTYHDTCSDGFADVDDPVKDQMAARLSDLSEMISNSLAIFSGYGEELPVQNRRRLMESDR 240
           LTYHDTCSDGFADVDDPVKDQMAARLSDLSEMISNSLAIFSGYG+ LPVQNRRRLMESD 
Sbjct: 181 LTYHDTCSDGFADVDDPVKDQMAARLSDLSEMISNSLAIFSGYGQGLPVQNRRRLMESDS 240

Query: 241 EWVAENGVAVAEGEFPGWVSGKDRRLLAAPISAIQADIVVAKDGSGKFTTVAEAIEAAPS 300
           EW AENGVAVAEGEFPGWVSGKDRRLLAAPISAIQADIVVAKDGSGKFTTVAEAIEAAPS
Sbjct: 241 EWAAENGVAVAEGEFPGWVSGKDRRLLAAPISAIQADIVVAKDGSGKFTTVAEAIEAAPS 300

Query: 301 SSGRRIVIYIKAGKYEEENLKVGRKKTNLMFVGDGKGITVISGGKSVFDKVTTFRTATF 360
           SSGRRIVIYIKAGKYEEENLKVGRKKTNLMFVGDGKGITVISGGKSVFDKVTTFRTATF
Sbjct: 301 SSGRRIVIYIKAGKYEEENLKVGRKKTNLMFVGDGKGITVISGGKSVFDKVTTFRTATF 359

BLAST of Carg14015 vs. NCBI nr
Match: XP_022996280.1 (probable pectinesterase/pectinesterase inhibitor 61 [Cucurbita maxima])

HSP 1 Score: 665.2 bits (1715), Expect = 3.3e-187
Identity = 349/362 (96.41%), Postives = 351/362 (96.96%), Query Frame = 0

Query: 1   MNNYNKLHSSASGDTPATFQVSHTPN---PPSSSASRRRTRILFVSLISILLITASAVSA 60
           MNNYNKLHSS SGDTPATFQVSHTPN   PPSSSASRRRTRILFVSLISILLITASAVSA
Sbjct: 1   MNNYNKLHSSGSGDTPATFQVSHTPNPPHPPSSSASRRRTRILFVSLISILLITASAVSA 60

Query: 61  AVLIHSHTSHFPHNKPTQAISHACSHTLYPSLCRSSLLSFPGSLSADVHRLVHISLNLTL 120
           AVLIHSH+SH PHNKPTQAISHACS TLYPSLCRSSLLSFPGSLSADVH LVHISLNLTL
Sbjct: 61  AVLIHSHSSHIPHNKPTQAISHACSRTLYPSLCRSSLLSFPGSLSADVHHLVHISLNLTL 120

Query: 121 QHLTKALYASSQIPVFQISNNPLAHSAYQDCIELLNDAVDAFSLSLFSTSASNHDIMTWL 180
           QHLTKALYASSQIPVFQISNNPLAHSAYQDCIELLNDAVDAFSLSLFSTSASNHDIMTWL
Sbjct: 121 QHLTKALYASSQIPVFQISNNPLAHSAYQDCIELLNDAVDAFSLSLFSTSASNHDIMTWL 180

Query: 181 SAALTYHDTCSDGFADVDDPVKDQMAARLSDLSEMISNSLAIFSGYGEELPVQNRRRLME 240
           SAALTYHDTCSDGFADVDDPVKDQMA+RLSDLSEMISNSLAIFSGYGE LPVQNRRRLME
Sbjct: 181 SAALTYHDTCSDGFADVDDPVKDQMASRLSDLSEMISNSLAIFSGYGEGLPVQNRRRLME 240

Query: 241 SDREWVAENGVAVAEGEFPGWVSGKDRRLLAAPISAIQADIVVAKDGSGKFTTVAEAIEA 300
           SDREW AENGVAVAEGEFPGWVSGKDRRLLAA ISAIQADIVVAKDGSGKF TVAEAIEA
Sbjct: 241 SDREWAAENGVAVAEGEFPGWVSGKDRRLLAASISAIQADIVVAKDGSGKFKTVAEAIEA 300

Query: 301 APSSSGRRIVIYIKAGKYEEENLKVGRKKTNLMFVGDGKGITVISGGKSVFDKVTTFRTA 360
           APSSSGRRIVIYIKAGKYEEENLKVGRKKTNLMFVGDGKGITVISGGKSVFDKVTTFRTA
Sbjct: 301 APSSSGRRIVIYIKAGKYEEENLKVGRKKTNLMFVGDGKGITVISGGKSVFDKVTTFRTA 360

BLAST of Carg14015 vs. ExPASy Swiss-Prot
Match: Q9FK05 (Probable pectinesterase/pectinesterase inhibitor 61 OS=Arabidopsis thaliana OX=3702 GN=PME61 PE=2 SV=1)

HSP 1 Score: 298.5 bits (763), Expect = 1.1e-79
Identity = 172/345 (49.86%), Postives = 221/345 (64.06%), Query Frame = 0

Query: 28  PSSSASRRRTRILFVSLISILLITASAVSAAVLIHSHTSHFPH--NKPTQAISHACSHTL 87
           P      +   ILF   + ++ +    + A +         P    KPTQAIS  CS +L
Sbjct: 24  PELQKKTKTKLILFTLAVLVVGVVCFGIFAGIRAVDSGKTEPKLTRKPTQAISRTCSKSL 83

Query: 88  YPSLCRSSLLSFPGSLSADVHRLVHISLNLTLQHLTKALYASSQIPVFQISNNPLAHSAY 147
           YP+LC  +LL FPGSL+AD + L+HIS N TLQ  +KALY SS I   Q+   P   SAY
Sbjct: 84  YPNLCIDTLLDFPGSLTADENELIHISFNATLQKFSKALYTSSTITYTQMP--PRVRSAY 143

Query: 148 QDCIELLNDAVDAFSLSLFST-----SASNHDIMTWLSAALTYHDTCSDGFADVD---DP 207
             C+ELL+D+VDA + +L S        S+ D+MTWLS+A+T HDTC+DGF +++     
Sbjct: 144 DSCLELLDDSVDALTRALSSVVVVSGDESHSDVMTWLSSAMTNHDTCTDGFDEIEGQGGE 203

Query: 208 VKDQMAARLSDLSEMISNSLAIFSGYGEEL---PVQNRRRLMESDREWVAENGVAVAEGE 267
           VKDQ+   + DLSEM+SN LAIF+G  ++L   PV N R+L+ ++              E
Sbjct: 204 VKDQVIGAVKDLSEMVSNCLAIFAGKVKDLSGVPVVNNRKLLGTEE-----------TEE 263

Query: 268 FPGWVSGKDRRLLAAPISAIQADIVVAKDGSGKFTTVAEAIEAAPSSSGRRIVIYIKAGK 327
            P W+  +DR LL  P SAIQADI V+KDGSG F T+AEAI+ AP  S RR VIY+KAG+
Sbjct: 264 LPNWLKREDRELLGTPTSAIQADITVSKDGSGTFKTIAEAIKKAPEHSSRRFVIYVKAGR 323

Query: 328 YEEENLKVGRKKTNLMFVGDGKGITVISGGKSVFDKVTTFRTATF 360
           YEEENLKVGRKKTNLMF+GDGKG TVI+GGKS+ D +TTF TATF
Sbjct: 324 YEEENLKVGRKKTNLMFIGDGKGKTVITGGKSIADDLTTFHTATF 355

BLAST of Carg14015 vs. ExPASy Swiss-Prot
Match: Q9M3B0 (Probable pectinesterase/pectinesterase inhibitor 34 OS=Arabidopsis thaliana OX=3702 GN=PME34 PE=2 SV=1)

HSP 1 Score: 293.9 bits (751), Expect = 2.6e-78
Identity = 178/381 (46.72%), Postives = 248/381 (65.09%), Query Frame = 0

Query: 9   SSASGDTPATFQVSHTPNPPSSSASRRRTRILFVSLISILLITASAVSAAV---LIHSHT 68
           ++++   P   QVS +  P +++   ++  ++   +++I LI A+A+ A V   L  + +
Sbjct: 16  TTSTTTAPILNQVSTSEQPENNNRRSKKKLVVSSIVLAISLILAAAIFAGVRSRLKLNQS 75

Query: 69  SHFPHNKPTQAISHACSHTLYPSLCRSSLLSFPGSLSADVHR-LVHISLNLTLQHLTKAL 128
                 KP+QAIS AC  T +P LC  SL+ FPGSL+A   + L+H+++N+TL H + AL
Sbjct: 76  VPGLARKPSQAISKACELTRFPELCVDSLMDFPGSLAASSSKDLIHVTVNMTLHHFSHAL 135

Query: 129 YASSQIPVFQISNNPLAHSAYQDCIELLNDAVDAFS---LSLFSTSASNHDIMTWLSAAL 188
           Y+S+ +    +   P A SAY  C+ELL+D+VDA S    S+ S+SA   D+ TWLSAAL
Sbjct: 136 YSSASLSF--VDMPPRARSAYDSCVELLDDSVDALSRALSSVVSSSAKPQDVTTWLSAAL 195

Query: 189 TYHDTCSDGFADVDD-PVKDQMAARLSDLSEMISNSLAIFSGY--GEE---LPVQNRRRL 248
           T HDTC++GF  VDD  VKD M A L +LSE++SN LAIFS    G++   +P+QNRR L
Sbjct: 196 TNHDTCTEGFDGVDDGGVKDHMTAALQNLSELVSNCLAIFSASHDGDDFAGVPIQNRRLL 255

Query: 249 MESDREWVAENGVAVAEGEFPGWVSGKDRRLLAAPISAIQADIVVAKDGSGKFTTVAEAI 308
                      GV   E +FP W+  K+R +L  P+S IQADI+V+KDG+G   T++EAI
Sbjct: 256 -----------GVEEREEKFPRWMRPKEREILEMPVSQIQADIIVSKDGNGTCKTISEAI 315

Query: 309 EAAPSSSGRRIVIYIKAGKYEEENLKVGRKKTNLMFVGDGKGITVISGGKSVFDKVTTFR 368
           + AP +S RRI+IY+KAG+YEE NLKVGRKK NLMFVGDGKG TVISGGKS+FD +TTF 
Sbjct: 316 KKAPQNSTRRIIIYVKAGRYEENNLKVGRKKINLMFVGDGKGKTVISGGKSIFDNITTFH 375

Query: 369 TATFGIYSPSLFS--ILFQNF 375
           TA+F        +  I F+N+
Sbjct: 376 TASFAATGAGFIARDITFENW 383

BLAST of Carg14015 vs. ExPASy Swiss-Prot
Match: Q43143 (Pectinesterase/pectinesterase inhibitor U1 OS=Solanum lycopersicum OX=4081 GN=PMEU1 PE=2 SV=1)

HSP 1 Score: 158.7 bits (400), Expect = 1.3e-37
Identity = 111/348 (31.90%), Postives = 192/348 (55.17%), Query Frame = 0

Query: 35  RRTRILFVSLISILLITASAVSAAVLIHSHTSHFPHNKPTQAIS--------HACSHTLY 94
           +R + +++++++ +L+ A+ +     + SH+ +   +    AIS         ACS+TL+
Sbjct: 16  KRKKKIYLAIVASVLLVAAVIGVVAGVKSHSKNSDDHADIMAISSSAHAIVKSACSNTLH 75

Query: 95  PSLCRSSLLS---FPGSLSADVHRLVHISLNLTLQHLTKALYASSQIPVFQISNNPLAHS 154
           P LC S++++   F   +++    ++ +SLN+T++ + +  YA  ++   +    P    
Sbjct: 76  PELCYSAIVNVSDFSKKVTSQ-KDVIELSLNITVKAVRRNYYAVKELIKTRKGLTPREKV 135

Query: 155 AYQDCIELLNDAVDAF-----SLSLFSTSAS----NHDIMTWLSAALTYHDTCSDGFA-- 214
           A  DC+E +++ +D        L L+    S      D+ T +S+A+T  +TC DGF+  
Sbjct: 136 ALHDCLETMDETLDELHTAVEDLELYPNKKSLKEHVEDLKTLISSAITNQETCLDGFSHD 195

Query: 215 DVDDPVKDQMAARLSDLSEMISNSLAIFSGYGEELPVQNRRRLM--ESDREWVAENGVAV 274
           + D  V+  +      + +M SN+LA+      +  + N  +L    ++R+ V +N    
Sbjct: 196 EADKKVRKVLLKGQKHVEKMCSNALAMICNM-TDTDIANEMKLSAPANNRKLVEDN---- 255

Query: 275 AEGEFPGWVSGKDRRLLAAPISAIQADIVVAKDGSGKFTTVAEAIEAAPSSSGRRIVIYI 334
             GE+P W+S  DRRLL +  S +  D+VVA DGSG + TV+EA+  AP  S +R VI I
Sbjct: 256 --GEWPEWLSAGDRRLLQS--STVTPDVVVAADGSGDYKTVSEAVRKAPEKSSKRYVIRI 315

Query: 335 KAGKYEEENLKVGRKKTNLMFVGDGKGITVISGGKSVFDKVTTFRTAT 359
           KAG Y  EN+ V +KKTN+MF+GDGK  T+I+  ++V D  TTF +AT
Sbjct: 316 KAGVY-RENVDVPKKKTNIMFMGDGKSNTIITASRNVQDGSTTFHSAT 352

BLAST of Carg14015 vs. ExPASy Swiss-Prot
Match: Q42534 (Pectinesterase 2 OS=Arabidopsis thaliana OX=3702 GN=PME2 PE=2 SV=2)

HSP 1 Score: 155.2 bits (391), Expect = 1.5e-36
Identity = 124/366 (33.88%), Postives = 188/366 (51.37%), Query Frame = 0

Query: 34  RRRTRILFVSLISILLITASAVSAAVLIHSHTSHFPHNKPT--QAISHA-----CSHTLY 93
           +   +++  S    LL+ AS V  A    + T+   + K T   + SHA     CS TLY
Sbjct: 15  KNNKKLILSSAAIALLLLASIVGIAA---TTTNQNKNQKITTLSSTSHAILKSVCSSTLY 74

Query: 94  PSLCRSSLLSFPGSLSADVHRLVHISLNLTLQHLTKALYASSQIPVFQISNNPLAHSAYQ 153
           P LC S++ +  G        ++  SLNLT + +    +A  ++   +    P   +A  
Sbjct: 75  PELCFSAVAATGGKELTSQKEVIEASLNLTTKAVKHNYFAVKKLIAKRKGLTPREVTALH 134

Query: 154 DCIELLNDAVDAFSLSL-------FSTSASNH--DIMTWLSAALTYHDTCSDGFA--DVD 213
           DC+E +++ +D   +++          S   H  D+ T +S+A+T   TC DGF+  D D
Sbjct: 135 DCLETIDETLDELHVAVEDLHQYPKQKSLRKHADDLKTLISSAITNQGTCLDGFSYDDAD 194

Query: 214 DPVKDQMAARLSDLSEMISNSLAIFSGYGE------ELPVQNRRRLMESDREWVAENGVA 273
             V+  +      +  M SN+LA+     E      EL  ++      ++R+     G  
Sbjct: 195 RKVRKALLKGQVHVEHMCSNALAMIKNMTETDIANFELRDKSSTFTNNNNRKLKEVTGDL 254

Query: 274 VAEGEFPGWVSGKDRRLLAAPISAIQADIVVAKDGSGKFTTVAEAIEAAPSSSGRRIVIY 333
            ++G +P W+S  DRRLL    S I+AD  VA DGSG FTTVA A+ AAP  S +R VI+
Sbjct: 255 DSDG-WPKWLSVGDRRLLQG--STIKADATVADDGSGDFTTVAAAVAAAPEKSNKRFVIH 314

Query: 334 IKAGKYEEENLKVGRKKTNLMFVGDGKGITVISGGKSVFDKVTTFRTATFGIYSPSLFS- 374
           IKAG Y  EN++V +KKTN+MF+GDG+G T+I+G ++V D  TTF +AT         + 
Sbjct: 315 IKAGVY-RENVEVTKKKTNIMFLGDGRGKTIITGSRNVVDGSTTFHSATVAAVGERFLAR 373

BLAST of Carg14015 vs. ExPASy Swiss-Prot
Match: O49006 (Pectinesterase/pectinesterase inhibitor 3 OS=Arabidopsis thaliana OX=3702 GN=PME3 PE=1 SV=2)

HSP 1 Score: 148.3 bits (373), Expect = 1.8e-34
Identity = 114/367 (31.06%), Postives = 189/367 (51.50%), Query Frame = 0

Query: 34  RRRTRILFVSLISILLITASAVSAAVLIHSHTSHFPHNKPTQAISHACSHTLYPSLCRSS 93
           + +  +L  + +++L + A A  +A    ++            +  +CS T YP LC S+
Sbjct: 16  KNKKLVLLSAAVALLFVAAVAGISAGASKANEKRTLSPSSHAVLRSSCSSTRYPELCISA 75

Query: 94  LLSFPGSLSADVHRLVHISLNLTLQHLTKALYASSQIPVFQISNNPLAHSAYQDCIELLN 153
           +++  G        ++  S+NLT+  +    +   ++   +    P   +A  DC+E ++
Sbjct: 76  VVTAGGVELTSQKDVIEASVNLTITAVEHNYFTVKKLIKKRKGLTPREKTALHDCLETID 135

Query: 154 DAVDAF-----SLSLFSTSAS--NH--DIMTWLSAALTYHDTCSDGFA--DVDDPVKDQM 213
           + +D        L L+ T  +   H  D+ T +S+A+T  +TC DGF+  D D  V+  +
Sbjct: 136 ETLDELHETVEDLHLYPTKKTLREHAGDLKTLISSAITNQETCLDGFSHDDADKQVRKAL 195

Query: 214 AARLSDLSEMISNSLAI--------FSGYGEELPV-QNRRRLMESDREW-----VAENGV 273
                 +  M SN+LA+         + + ++  +  N R+L E ++E      +A  G 
Sbjct: 196 LKGQIHVEHMCSNALAMIKNMTDTDIANFEQKAKITSNNRKLKEENQETTVAVDIAGAGE 255

Query: 274 AVAEGEFPGWVSGKDRRLLAAPISAIQADIVVAKDGSGKFTTVAEAIEAAPSSSGRRIVI 333
             +EG +P W+S  DRRLL    S ++AD  VA DGSG F TVA A+ AAP +S +R VI
Sbjct: 256 LDSEG-WPTWLSAGDRRLLQG--SGVKADATVAADGSGTFKTVAAAVAAAPENSNKRYVI 315

Query: 334 YIKAGKYEEENLKVGRKKTNLMFVGDGKGITVISGGKSVFDKVTTFRTATFGIYSPSLFS 374
           +IKAG Y  EN++V +KK N+MF+GDG+  T+I+G ++V D  TTF +AT         +
Sbjct: 316 HIKAGVY-RENVEVAKKKKNIMFMGDGRTRTIITGSRNVVDGSTTFHSATVAAVGERFLA 375

BLAST of Carg14015 vs. ExPASy TrEMBL
Match: A0A6J1H2K1 (Pectinesterase OS=Cucurbita moschata OX=3662 GN=LOC111459120 PE=3 SV=1)

HSP 1 Score: 679.9 bits (1753), Expect = 6.3e-192
Identity = 353/359 (98.33%), Postives = 355/359 (98.89%), Query Frame = 0

Query: 1   MNNYNKLHSSASGDTPATFQVSHTPNPPSSSASRRRTRILFVSLISILLITASAVSAAVL 60
           MNNYNKLHSSASGDTPATFQVSHTPNPPSSSASRRRTRILFVSLISILLITASAVSAAVL
Sbjct: 1   MNNYNKLHSSASGDTPATFQVSHTPNPPSSSASRRRTRILFVSLISILLITASAVSAAVL 60

Query: 61  IHSHTSHFPHNKPTQAISHACSHTLYPSLCRSSLLSFPGSLSADVHRLVHISLNLTLQHL 120
           +HS +SHFPHNKPTQAISHACSHTLYPSLCRSSLLSFPGSLSADVHRLVHISLNLTLQHL
Sbjct: 61  LHSQSSHFPHNKPTQAISHACSHTLYPSLCRSSLLSFPGSLSADVHRLVHISLNLTLQHL 120

Query: 121 TKALYASSQIPVFQISNNPLAHSAYQDCIELLNDAVDAFSLSLFSTSASNHDIMTWLSAA 180
           TKALYASSQIPVFQISNNPLAHSAYQDCIELLNDAVDAFSLSLFSTSASNHDIMTWLSAA
Sbjct: 121 TKALYASSQIPVFQISNNPLAHSAYQDCIELLNDAVDAFSLSLFSTSASNHDIMTWLSAA 180

Query: 181 LTYHDTCSDGFADVDDPVKDQMAARLSDLSEMISNSLAIFSGYGEELPVQNRRRLMESDR 240
           LTYHDTCSDGFADVDDPVKDQMAARLSDLSEMISNSLAIFSGYGE LPVQNRRRLMESDR
Sbjct: 181 LTYHDTCSDGFADVDDPVKDQMAARLSDLSEMISNSLAIFSGYGEGLPVQNRRRLMESDR 240

Query: 241 EWVAENGVAVAEGEFPGWVSGKDRRLLAAPISAIQADIVVAKDGSGKFTTVAEAIEAAPS 300
           EW AENGVAVAEGEFPGWVSGKDRRLLAAPISAIQADIVVAKDGSGKF TVAEAIEAAPS
Sbjct: 241 EWAAENGVAVAEGEFPGWVSGKDRRLLAAPISAIQADIVVAKDGSGKFKTVAEAIEAAPS 300

Query: 301 SSGRRIVIYIKAGKYEEENLKVGRKKTNLMFVGDGKGITVISGGKSVFDKVTTFRTATF 360
           SSGRRIVIYIKAGKYEEENLKVGRKKTNLMFVGDGKGITVISGGKSVFDKVTTFRTATF
Sbjct: 301 SSGRRIVIYIKAGKYEEENLKVGRKKTNLMFVGDGKGITVISGGKSVFDKVTTFRTATF 359

BLAST of Carg14015 vs. ExPASy TrEMBL
Match: A0A6J1K6C1 (Pectinesterase OS=Cucurbita maxima OX=3661 GN=LOC111491552 PE=3 SV=1)

HSP 1 Score: 665.2 bits (1715), Expect = 1.6e-187
Identity = 349/362 (96.41%), Postives = 351/362 (96.96%), Query Frame = 0

Query: 1   MNNYNKLHSSASGDTPATFQVSHTPN---PPSSSASRRRTRILFVSLISILLITASAVSA 60
           MNNYNKLHSS SGDTPATFQVSHTPN   PPSSSASRRRTRILFVSLISILLITASAVSA
Sbjct: 1   MNNYNKLHSSGSGDTPATFQVSHTPNPPHPPSSSASRRRTRILFVSLISILLITASAVSA 60

Query: 61  AVLIHSHTSHFPHNKPTQAISHACSHTLYPSLCRSSLLSFPGSLSADVHRLVHISLNLTL 120
           AVLIHSH+SH PHNKPTQAISHACS TLYPSLCRSSLLSFPGSLSADVH LVHISLNLTL
Sbjct: 61  AVLIHSHSSHIPHNKPTQAISHACSRTLYPSLCRSSLLSFPGSLSADVHHLVHISLNLTL 120

Query: 121 QHLTKALYASSQIPVFQISNNPLAHSAYQDCIELLNDAVDAFSLSLFSTSASNHDIMTWL 180
           QHLTKALYASSQIPVFQISNNPLAHSAYQDCIELLNDAVDAFSLSLFSTSASNHDIMTWL
Sbjct: 121 QHLTKALYASSQIPVFQISNNPLAHSAYQDCIELLNDAVDAFSLSLFSTSASNHDIMTWL 180

Query: 181 SAALTYHDTCSDGFADVDDPVKDQMAARLSDLSEMISNSLAIFSGYGEELPVQNRRRLME 240
           SAALTYHDTCSDGFADVDDPVKDQMA+RLSDLSEMISNSLAIFSGYGE LPVQNRRRLME
Sbjct: 181 SAALTYHDTCSDGFADVDDPVKDQMASRLSDLSEMISNSLAIFSGYGEGLPVQNRRRLME 240

Query: 241 SDREWVAENGVAVAEGEFPGWVSGKDRRLLAAPISAIQADIVVAKDGSGKFTTVAEAIEA 300
           SDREW AENGVAVAEGEFPGWVSGKDRRLLAA ISAIQADIVVAKDGSGKF TVAEAIEA
Sbjct: 241 SDREWAAENGVAVAEGEFPGWVSGKDRRLLAASISAIQADIVVAKDGSGKFKTVAEAIEA 300

Query: 301 APSSSGRRIVIYIKAGKYEEENLKVGRKKTNLMFVGDGKGITVISGGKSVFDKVTTFRTA 360
           APSSSGRRIVIYIKAGKYEEENLKVGRKKTNLMFVGDGKGITVISGGKSVFDKVTTFRTA
Sbjct: 301 APSSSGRRIVIYIKAGKYEEENLKVGRKKTNLMFVGDGKGITVISGGKSVFDKVTTFRTA 360

BLAST of Carg14015 vs. ExPASy TrEMBL
Match: A0A6J1E2V1 (Pectinesterase OS=Cucurbita moschata OX=3662 GN=LOC111430323 PE=3 SV=1)

HSP 1 Score: 539.3 bits (1388), Expect = 1.3e-149
Identity = 294/370 (79.46%), Postives = 325/370 (87.84%), Query Frame = 0

Query: 2   NNYNKLHSSASGDTPA-TFQVSH---TPNPPSSSASRRRTRILFVSLISILLITASAVSA 61
           NNYN LHSS SGDTPA +F+VS+    P PP S +S RR RIL +S  SILLITASAVSA
Sbjct: 3   NNYNMLHSSHSGDTPASSFRVSNDHSDPLPPPSPSSSRR-RILLISFFSILLITASAVSA 62

Query: 62  AVLIHSH-----TSHFPHNKPTQAISHACSHTLYPSLCRSSLLSFPGSLSADVHRLVHIS 121
            VL+HS       SHFPHNKPTQAIS ACS TLYPSLC SSLLS PGSLSADVH LVHIS
Sbjct: 63  VVLVHSRIAASTESHFPHNKPTQAISRACSLTLYPSLCLSSLLSAPGSLSADVHDLVHIS 122

Query: 122 LNLTLQHLTKALYASSQIPVFQISNNPLAHSAYQDCIELLNDAVDAFSLSLFSTSASNHD 181
           LNLTLQHLTKALY++SQIP+ QISN+PLA SAY+DC+ELLNDAV+AFSLSLFST+A+NHD
Sbjct: 123 LNLTLQHLTKALYSTSQIPISQISNDPLAQSAYEDCMELLNDAVEAFSLSLFSTTATNHD 182

Query: 182 IMTWLSAALTYHDTCSDGFADVDDPVKDQMAARLSDLSEMISNSLAIFSGY-GEELPVQN 241
           +MTWLSAALTYHDTC+DGF+DV DP+KDQ+ A+LSDLSEMISNSLAIFSG+ G++LPV+N
Sbjct: 183 VMTWLSAALTYHDTCTDGFSDVADPIKDQITAKLSDLSEMISNSLAIFSGFGGDDLPVEN 242

Query: 242 --RRRLMESDREWVAENGVAVAEGEFPGWVSGKDRRLLAAPISAIQADIVVAKDGSGKFT 301
             RRRLMESD +W+AE      +G+FP WVSGKDRRLLAAPIS+IQADIVVAKDGSGKF 
Sbjct: 243 RRRRRLMESDEQWLAEEN--NGDGDFPRWVSGKDRRLLAAPISSIQADIVVAKDGSGKFK 302

Query: 302 TVAEAIEAAPSSSGRRIVIYIKAGKYEEENLKVGRKKTNLMFVGDGKGITVISGGKSVFD 360
           TVAEAI AAPSSSGRRIVIYIKAGKYEEENLKVGRKKTNLMFVGDGKGITVISGGKSV+D
Sbjct: 303 TVAEAIAAAPSSSGRRIVIYIKAGKYEEENLKVGRKKTNLMFVGDGKGITVISGGKSVYD 362

BLAST of Carg14015 vs. ExPASy TrEMBL
Match: A0A6J1IA52 (Pectinesterase OS=Cucurbita maxima OX=3661 GN=LOC111471502 PE=3 SV=1)

HSP 1 Score: 534.6 bits (1376), Expect = 3.3e-148
Identity = 291/369 (78.86%), Postives = 318/369 (86.18%), Query Frame = 0

Query: 2   NNYNKLHSSASGDTPAT-FQVS--HTPNPPSSSASRRRTRILFVSLISILLITASAVSAA 61
           NNYNKLHSS SGDTP T F+VS  H+  PP    S    RIL +S  SILLITAS VSA 
Sbjct: 3   NNYNKLHSSRSGDTPVTSFRVSNDHSNPPPPPPPSSSHRRILLISFFSILLITASTVSAV 62

Query: 62  VLIHSH-----TSHFPHNKPTQAISHACSHTLYPSLCRSSLLSFPGSLSADVHRLVHISL 121
           VL+HS       SHFPHNKPTQAIS ACS TLYPSLC SSLLS PGSLSADVH LVHISL
Sbjct: 63  VLVHSRITTSTESHFPHNKPTQAISRACSLTLYPSLCISSLLSAPGSLSADVHDLVHISL 122

Query: 122 NLTLQHLTKALYASSQIPVFQISNNPLAHSAYQDCIELLNDAVDAFSLSLFSTSASNHDI 181
           NLTLQHLTKALY++SQIP+ QISN+PLA SAY+DC+ELLNDAV+AFSLSLFST+A+NHD+
Sbjct: 123 NLTLQHLTKALYSTSQIPISQISNDPLAQSAYEDCMELLNDAVEAFSLSLFSTTATNHDV 182

Query: 182 MTWLSAALTYHDTCSDGFADVDDPVKDQMAARLSDLSEMISNSLAIFSGY-GEELPVQN- 241
           MTWLSAALTYHDTC+DGF+DV DPVKDQ+ A+LSDLSEMISNSLAIFSG+ G +LPV+N 
Sbjct: 183 MTWLSAALTYHDTCTDGFSDVADPVKDQITAKLSDLSEMISNSLAIFSGFGGGDLPVENR 242

Query: 242 -RRRLMESDREWVAENGVAVAEGEFPGWVSGKDRRLLAAPISAIQADIVVAKDGSGKFTT 301
            RRRLMESD +W+AE      +G+FP WVSGKDRRLLAAPIS+IQADIVVAKDGSGKF T
Sbjct: 243 RRRRLMESDEQWLAEEN--NGDGDFPRWVSGKDRRLLAAPISSIQADIVVAKDGSGKFKT 302

Query: 302 VAEAIEAAPSSSGRRIVIYIKAGKYEEENLKVGRKKTNLMFVGDGKGITVISGGKSVFDK 360
           VAEAI AAPSSS RRIVIYIKAGKYEEENLKVGRKKTNLMFVGDGKGITVISGGKSV+DK
Sbjct: 303 VAEAIAAAPSSSSRRIVIYIKAGKYEEENLKVGRKKTNLMFVGDGKGITVISGGKSVYDK 362

BLAST of Carg14015 vs. ExPASy TrEMBL
Match: A0A0A0KJG1 (Pectinesterase OS=Cucumis sativus OX=3659 GN=Csa_5G139430 PE=3 SV=1)

HSP 1 Score: 516.9 bits (1330), Expect = 7.1e-143
Identity = 284/369 (76.96%), Postives = 317/369 (85.91%), Query Frame = 0

Query: 2   NNYNKLHSSASGDT--PATFQVSHTPNPPSSSASRRRTRILFVSLISILLITASAVSAAV 61
           N+YNKLH+S S +T   A+F++S    PP SS+ RRR+RIL +S +S+LLI AS VSAA+
Sbjct: 5   NSYNKLHTSHSANTTPAASFRLSGNDPPPPSSSLRRRSRILIISFMSVLLIAASVVSAAI 64

Query: 62  LIHSHTS----HFPHNKPTQAISHACSHTLYPSLCRSSLLSFPGSLSADVHRLVHISLNL 121
           L+ SH +    HFPHNKP+QAIS ACSHTLYPSLC SSLLSFPG+ SADVH LVHISLNL
Sbjct: 65  LVRSHMTSSQPHFPHNKPSQAISRACSHTLYPSLCLSSLLSFPGAQSADVHDLVHISLNL 124

Query: 122 TLQHLTKALYASSQIPVFQISNNPLAHSAYQDCIELLNDAVDAFSLSLFSTSASNHDIMT 181
           TLQHLTKALY++SQIPV QIS +PLAHSAY+DC+ELLNDA+DAFSLSLFS  ASNHDIMT
Sbjct: 125 TLQHLTKALYSTSQIPVLQISKDPLAHSAYEDCMELLNDAIDAFSLSLFSKDASNHDIMT 184

Query: 182 WLSAALTYHDTCSDGFADVDD-PVKDQMAARLSDLSEMISNSLAIFSGY-GEELPVQN-- 241
           WLSAALTYHDTC+ GF DV D  VKD++ A+LSDLSEMISNSLAIFSG+ G +LPV+N  
Sbjct: 185 WLSAALTYHDTCTAGFQDVADLGVKDEVEAKLSDLSEMISNSLAIFSGFGGGDLPVENRK 244

Query: 242 RRRLMESD-REWVAENGVAVAEGEFPGWVSGKDRRLLAAPISAIQADIVVAKDGSGKFTT 301
           RRRLMES    W AENG    EG FP W+SGKDRRLLAAP+S IQADIVVAKDGSGKF T
Sbjct: 245 RRRLMESSTTSWAAENG-GDHEG-FPAWLSGKDRRLLAAPLSTIQADIVVAKDGSGKFKT 304

Query: 302 VAEAIEAAPSSSGRRIVIYIKAGKYEEENLKVGRKKTNLMFVGDGKGITVISGGKSVFDK 360
           VAEAIEAAPSSSGRRI+IYIKAGKYEEENLKVGRKKTNLMFVGDGKGITVISGGKSV+DK
Sbjct: 305 VAEAIEAAPSSSGRRIIIYIKAGKYEEENLKVGRKKTNLMFVGDGKGITVISGGKSVYDK 364

BLAST of Carg14015 vs. TAIR 10
Match: AT5G53370.1 (pectin methylesterase PCR fragment F )

HSP 1 Score: 298.5 bits (763), Expect = 7.6e-81
Identity = 172/345 (49.86%), Postives = 221/345 (64.06%), Query Frame = 0

Query: 28  PSSSASRRRTRILFVSLISILLITASAVSAAVLIHSHTSHFPH--NKPTQAISHACSHTL 87
           P      +   ILF   + ++ +    + A +         P    KPTQAIS  CS +L
Sbjct: 24  PELQKKTKTKLILFTLAVLVVGVVCFGIFAGIRAVDSGKTEPKLTRKPTQAISRTCSKSL 83

Query: 88  YPSLCRSSLLSFPGSLSADVHRLVHISLNLTLQHLTKALYASSQIPVFQISNNPLAHSAY 147
           YP+LC  +LL FPGSL+AD + L+HIS N TLQ  +KALY SS I   Q+   P   SAY
Sbjct: 84  YPNLCIDTLLDFPGSLTADENELIHISFNATLQKFSKALYTSSTITYTQMP--PRVRSAY 143

Query: 148 QDCIELLNDAVDAFSLSLFST-----SASNHDIMTWLSAALTYHDTCSDGFADVD---DP 207
             C+ELL+D+VDA + +L S        S+ D+MTWLS+A+T HDTC+DGF +++     
Sbjct: 144 DSCLELLDDSVDALTRALSSVVVVSGDESHSDVMTWLSSAMTNHDTCTDGFDEIEGQGGE 203

Query: 208 VKDQMAARLSDLSEMISNSLAIFSGYGEEL---PVQNRRRLMESDREWVAENGVAVAEGE 267
           VKDQ+   + DLSEM+SN LAIF+G  ++L   PV N R+L+ ++              E
Sbjct: 204 VKDQVIGAVKDLSEMVSNCLAIFAGKVKDLSGVPVVNNRKLLGTEE-----------TEE 263

Query: 268 FPGWVSGKDRRLLAAPISAIQADIVVAKDGSGKFTTVAEAIEAAPSSSGRRIVIYIKAGK 327
            P W+  +DR LL  P SAIQADI V+KDGSG F T+AEAI+ AP  S RR VIY+KAG+
Sbjct: 264 LPNWLKREDRELLGTPTSAIQADITVSKDGSGTFKTIAEAIKKAPEHSSRRFVIYVKAGR 323

Query: 328 YEEENLKVGRKKTNLMFVGDGKGITVISGGKSVFDKVTTFRTATF 360
           YEEENLKVGRKKTNLMF+GDGKG TVI+GGKS+ D +TTF TATF
Sbjct: 324 YEEENLKVGRKKTNLMFIGDGKGKTVITGGKSIADDLTTFHTATF 355

BLAST of Carg14015 vs. TAIR 10
Match: AT3G49220.1 (Plant invertase/pectin methylesterase inhibitor superfamily )

HSP 1 Score: 293.9 bits (751), Expect = 1.9e-79
Identity = 178/381 (46.72%), Postives = 248/381 (65.09%), Query Frame = 0

Query: 9   SSASGDTPATFQVSHTPNPPSSSASRRRTRILFVSLISILLITASAVSAAV---LIHSHT 68
           ++++   P   QVS +  P +++   ++  ++   +++I LI A+A+ A V   L  + +
Sbjct: 16  TTSTTTAPILNQVSTSEQPENNNRRSKKKLVVSSIVLAISLILAAAIFAGVRSRLKLNQS 75

Query: 69  SHFPHNKPTQAISHACSHTLYPSLCRSSLLSFPGSLSADVHR-LVHISLNLTLQHLTKAL 128
                 KP+QAIS AC  T +P LC  SL+ FPGSL+A   + L+H+++N+TL H + AL
Sbjct: 76  VPGLARKPSQAISKACELTRFPELCVDSLMDFPGSLAASSSKDLIHVTVNMTLHHFSHAL 135

Query: 129 YASSQIPVFQISNNPLAHSAYQDCIELLNDAVDAFS---LSLFSTSASNHDIMTWLSAAL 188
           Y+S+ +    +   P A SAY  C+ELL+D+VDA S    S+ S+SA   D+ TWLSAAL
Sbjct: 136 YSSASLSF--VDMPPRARSAYDSCVELLDDSVDALSRALSSVVSSSAKPQDVTTWLSAAL 195

Query: 189 TYHDTCSDGFADVDD-PVKDQMAARLSDLSEMISNSLAIFSGY--GEE---LPVQNRRRL 248
           T HDTC++GF  VDD  VKD M A L +LSE++SN LAIFS    G++   +P+QNRR L
Sbjct: 196 TNHDTCTEGFDGVDDGGVKDHMTAALQNLSELVSNCLAIFSASHDGDDFAGVPIQNRRLL 255

Query: 249 MESDREWVAENGVAVAEGEFPGWVSGKDRRLLAAPISAIQADIVVAKDGSGKFTTVAEAI 308
                      GV   E +FP W+  K+R +L  P+S IQADI+V+KDG+G   T++EAI
Sbjct: 256 -----------GVEEREEKFPRWMRPKEREILEMPVSQIQADIIVSKDGNGTCKTISEAI 315

Query: 309 EAAPSSSGRRIVIYIKAGKYEEENLKVGRKKTNLMFVGDGKGITVISGGKSVFDKVTTFR 368
           + AP +S RRI+IY+KAG+YEE NLKVGRKK NLMFVGDGKG TVISGGKS+FD +TTF 
Sbjct: 316 KKAPQNSTRRIIIYVKAGRYEENNLKVGRKKINLMFVGDGKGKTVISGGKSIFDNITTFH 375

Query: 369 TATFGIYSPSLFS--ILFQNF 375
           TA+F        +  I F+N+
Sbjct: 376 TASFAATGAGFIARDITFENW 383

BLAST of Carg14015 vs. TAIR 10
Match: AT1G53830.1 (pectin methylesterase 2 )

HSP 1 Score: 155.2 bits (391), Expect = 1.0e-37
Identity = 124/366 (33.88%), Postives = 188/366 (51.37%), Query Frame = 0

Query: 34  RRRTRILFVSLISILLITASAVSAAVLIHSHTSHFPHNKPT--QAISHA-----CSHTLY 93
           +   +++  S    LL+ AS V  A    + T+   + K T   + SHA     CS TLY
Sbjct: 15  KNNKKLILSSAAIALLLLASIVGIAA---TTTNQNKNQKITTLSSTSHAILKSVCSSTLY 74

Query: 94  PSLCRSSLLSFPGSLSADVHRLVHISLNLTLQHLTKALYASSQIPVFQISNNPLAHSAYQ 153
           P LC S++ +  G        ++  SLNLT + +    +A  ++   +    P   +A  
Sbjct: 75  PELCFSAVAATGGKELTSQKEVIEASLNLTTKAVKHNYFAVKKLIAKRKGLTPREVTALH 134

Query: 154 DCIELLNDAVDAFSLSL-------FSTSASNH--DIMTWLSAALTYHDTCSDGFA--DVD 213
           DC+E +++ +D   +++          S   H  D+ T +S+A+T   TC DGF+  D D
Sbjct: 135 DCLETIDETLDELHVAVEDLHQYPKQKSLRKHADDLKTLISSAITNQGTCLDGFSYDDAD 194

Query: 214 DPVKDQMAARLSDLSEMISNSLAIFSGYGE------ELPVQNRRRLMESDREWVAENGVA 273
             V+  +      +  M SN+LA+     E      EL  ++      ++R+     G  
Sbjct: 195 RKVRKALLKGQVHVEHMCSNALAMIKNMTETDIANFELRDKSSTFTNNNNRKLKEVTGDL 254

Query: 274 VAEGEFPGWVSGKDRRLLAAPISAIQADIVVAKDGSGKFTTVAEAIEAAPSSSGRRIVIY 333
            ++G +P W+S  DRRLL    S I+AD  VA DGSG FTTVA A+ AAP  S +R VI+
Sbjct: 255 DSDG-WPKWLSVGDRRLLQG--STIKADATVADDGSGDFTTVAAAVAAAPEKSNKRFVIH 314

Query: 334 IKAGKYEEENLKVGRKKTNLMFVGDGKGITVISGGKSVFDKVTTFRTATFGIYSPSLFS- 374
           IKAG Y  EN++V +KKTN+MF+GDG+G T+I+G ++V D  TTF +AT         + 
Sbjct: 315 IKAGVY-RENVEVTKKKTNIMFLGDGRGKTIITGSRNVVDGSTTFHSATVAAVGERFLAR 373

BLAST of Carg14015 vs. TAIR 10
Match: AT3G14310.1 (pectin methylesterase 3 )

HSP 1 Score: 148.3 bits (373), Expect = 1.3e-35
Identity = 114/367 (31.06%), Postives = 189/367 (51.50%), Query Frame = 0

Query: 34  RRRTRILFVSLISILLITASAVSAAVLIHSHTSHFPHNKPTQAISHACSHTLYPSLCRSS 93
           + +  +L  + +++L + A A  +A    ++            +  +CS T YP LC S+
Sbjct: 16  KNKKLVLLSAAVALLFVAAVAGISAGASKANEKRTLSPSSHAVLRSSCSSTRYPELCISA 75

Query: 94  LLSFPGSLSADVHRLVHISLNLTLQHLTKALYASSQIPVFQISNNPLAHSAYQDCIELLN 153
           +++  G        ++  S+NLT+  +    +   ++   +    P   +A  DC+E ++
Sbjct: 76  VVTAGGVELTSQKDVIEASVNLTITAVEHNYFTVKKLIKKRKGLTPREKTALHDCLETID 135

Query: 154 DAVDAF-----SLSLFSTSAS--NH--DIMTWLSAALTYHDTCSDGFA--DVDDPVKDQM 213
           + +D        L L+ T  +   H  D+ T +S+A+T  +TC DGF+  D D  V+  +
Sbjct: 136 ETLDELHETVEDLHLYPTKKTLREHAGDLKTLISSAITNQETCLDGFSHDDADKQVRKAL 195

Query: 214 AARLSDLSEMISNSLAI--------FSGYGEELPV-QNRRRLMESDREW-----VAENGV 273
                 +  M SN+LA+         + + ++  +  N R+L E ++E      +A  G 
Sbjct: 196 LKGQIHVEHMCSNALAMIKNMTDTDIANFEQKAKITSNNRKLKEENQETTVAVDIAGAGE 255

Query: 274 AVAEGEFPGWVSGKDRRLLAAPISAIQADIVVAKDGSGKFTTVAEAIEAAPSSSGRRIVI 333
             +EG +P W+S  DRRLL    S ++AD  VA DGSG F TVA A+ AAP +S +R VI
Sbjct: 256 LDSEG-WPTWLSAGDRRLLQG--SGVKADATVAADGSGTFKTVAAAVAAAPENSNKRYVI 315

Query: 334 YIKAGKYEEENLKVGRKKTNLMFVGDGKGITVISGGKSVFDKVTTFRTATFGIYSPSLFS 374
           +IKAG Y  EN++V +KK N+MF+GDG+  T+I+G ++V D  TTF +AT         +
Sbjct: 316 HIKAGVY-RENVEVAKKKKNIMFMGDGRTRTIITGSRNVVDGSTTFHSATVAAVGERFLA 375

BLAST of Carg14015 vs. TAIR 10
Match: AT3G05610.1 (Plant invertase/pectin methylesterase inhibitor superfamily )

HSP 1 Score: 145.6 bits (366), Expect = 8.2e-35
Identity = 109/349 (31.23%), Postives = 174/349 (49.86%), Query Frame = 0

Query: 33  SRRRTRILFVSLISILLIT-ASAVSAAVLIHSHTSHFPH----NKPTQAISHACSHTLYP 92
           S+R+ R + +++ S+LLI+   AV+  V ++ H          N   +A+   C+ T Y 
Sbjct: 9   SKRKRRYIVITISSVLLISMVVAVTVGVSLNKHDGDSKGKAEVNASVKAVKDVCAPTDYR 68

Query: 93  SLCRSSLLSFPGSLSADVHRLVHISLNLTLQHLTKALYASSQIPVFQISNNPLAHSAYQD 152
             C  +L+   G  + D   LV  + N+T++ +T A  A     + ++  +     A   
Sbjct: 69  KTCEDTLIK-NGKNTTDPMELVKTAFNVTMKQITDA--AKKSQTIMELQKDSRTRMALDQ 128

Query: 153 CIELLNDAVDAFSLSLFSTSASNHDIM--------TWLSAALTYHDTCSDGFADVDDPVK 212
           C EL++ A+D  S S          ++         WLSAA+++ +TC +GF        
Sbjct: 129 CKELMDYALDELSNSFEELGKFEFHLLDEALINLRIWLSAAISHEETCLEGFQGTQGNAG 188

Query: 213 DQMAARLSDLSEMISNSLAIFSGYGE-----ELPVQNRRRLMESDREWVAENGVAVAEGE 272
           + M   L    E+  N LAI S         ++P  N RRL+              AEG 
Sbjct: 189 ETMKKALKTAIELTHNGLAIISEMSNFVGQMQIPGLNSRRLL--------------AEG- 248

Query: 273 FPGWVSGKDRRLL--AAPISAIQADIVVAKDGSGKFTTVAEAIEAAPSSSGRRIVIYIKA 332
           FP WV  + R+LL  AA  S ++ DIVVA+DGSG++ T+ EA++  P       V++IKA
Sbjct: 249 FPSWVDQRGRKLLQAAAAYSDVKPDIVVAQDGSGQYKTINEALQFVPKKRNTTFVVHIKA 308

Query: 333 GKYEEENLKVGRKKTNLMFVGDGKGITVISGGKSVFDKVTTFRTATFGI 362
           G Y +E ++V +  ++L+F+GDG   T+ISG K+  D +TT+RTAT  I
Sbjct: 309 GLY-KEYVQVNKTMSHLVFIGDGPDKTIISGNKNYKDGITTYRTATVAI 338