Carg13998 (gene) Silver-seed gourd (SMH-JMG-627) v2

Overview


Name	Carg13998
Type	gene
Organism	Cucurbita argyrosperma subsp. argyrosperma cv. SMH-JMG-627 (Silver-seed gourd (SMH-JMG-627) v2)
Description	cytochrome b5-like
Location	Carg_Chr02: 6663140 .. 6665394 (+)
RNA-Seq Expression	Carg13998
Synteny	Carg13998

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRCDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

TTTTGACTTCGTAATTAACTGCAAATCAAGGGCCATTTTGCCGGCCTCGTTTTTGTTCTTTCATTGTTACTATAAAACGATTTGTTTCCTCCGTTGCCAAATCGGGAACTAATCTGTAATCAGGCTTCGCCACAAACAGGATCCGATCAAGTCGTTGCCTCTTCAATCCTTTTTCATCTGTGAGTGAATCTGTTCCTTACGAATCCATTTCGATTTCGATGGTTTTGATTACTTCTATTGGGGTTTTGGATGTGAAATTTGAAGTTCTTGGCCGCTCCTCTTCGATTGTTTGTTGATTGTTGCAGTGATATATGGCTTCCGATCCGAAGGTTCATCTGTTCGAAGAAGTGGCAAAGCATAACAAAACGAAGGATTGTTGGCTTGTAATCTCTGGAAAGGTTGAATCTCTCTTGGTTTATTGTTCGTATCGTTTTATTTAGCATTGGATATCAATTTTCTGGTGATGTTTTTGACGGAAAATTCTTGCGAACTCTGTTTTGTCATCGAATATCCTCTAAAAGAGTTTGATATAGGCGTGTTGGCTTTGGGGGTTTCAGTGTTTTACATGAATGTTCAAATTCAAATATCTTCTTCCTTGTTCTTCACTGAGCCGGATTCGTTTCTATGTCTTAATATCATTTTGTCATAGTAATATCAAATGGGAAGAAAATGGTGGTGAGCTGCAGCATATGAAGATTAAGTAAAGCAGTGGCATTGTTTTCATATACGCTAAAGAGTTTGTCCAACCAATTTTGAGCTTGAAAAACAACATTTATTGGTTGAAAATTGTCCTAAACTTCACTATTTACTATGAGGTTTCAGGCAATTGTTTCTTGTCCCCATAAGAAATCATAGCTTCGTTGAACTGATTGTTATAAAATGCTTAGAAGAAGTATACCACCCAAGGGGTTGGGCGTGGTGGTCTAGACTTGGGGTTAACCAGAGTGGTGTGTTTAAAGCCTGCCTTTTTGGCTACGTTGTGAAGATATTTTATTTGTCACATTCATGGTGAATATTGAGAGCTTTTGGGATCAAGCTAAATGTTTAAACTGTTGATTAATAGAGATGAGGTAATTTAGAAATTCTTGACCTGTTCTCTGTGAGATCCCACATCGGTTGGAGAGAGGAACGAAGCATTCCTTAAAAGAGTGTGGAAACCTTCTCCCTAACAGACGCGTTTTAAAACCTTGAGAGGAAGCTCAAAAGGGAAAGCCATAGAGGACAATATCTGTTTGTGATGGGCTTGGGCTGTTACAAATGGTATTAGAGTCAGGCTTCGGGTGGTATGCTAGCAAGGATGTTGGGTCCCTAAGGGGGGTGGATTGTGAGATCTCACGTCGGTTGGAAAGGAGAATGAAGTATTCTTTATAAGGGTGTGAAAACCTCTCCCTAATATAAGGGTGTGGAAACCTCTCCCTAATATACGCGTTGTAAAACCTTGAGGGGATGCCCAAAAGGGGAAGTCCAAAAGGACAATATCTGCTAGCGGTGAGCTTGAGCTGTTACATTCTCGTTTAAATATTTTCTATCATGCATTCAAATTGTGGTGGTTACATTTGTGGAATGTTTAAAATGGTTTGAGATCGTGTTCAAGTCAACTTAAGTATAGTTCAATGGGTCAAAATATATGTCATTGCCAACAAGTCTTAGTTAGAGGGTTCATATATTTCCACTCCACTCCACATGTTGAACTCGAAAGGGAAAACAAAGATCACTTGTTCATGAGGTGTTACTAAGCTGGTTTGTTGTGTGTATCATAAGTAATTTATGTTATGTGGAAGCTTTAGATATCTTTTATTATGTTTGATTGTGGCTTTGGCTTTAGGTTTATGATGTAACTCCTTTCATGGAGGATCACCCCGGTGGCGATGAAGTATTGTTGTCTGCAACGGGTAAGCCTCAGAGTTCCCTTATATCGAATATATGTTTCTATTATTTTCAAGTCTCTTGCAAATGATGATTAGGATGGATACAATCAATGCTGTTCGTTGGAATCTTTTGCAGGAAAAGATGCCTCGAACGATTTCGAGGATGTCGGGCACAGTGATTCAGCAAGAGAAATGATGGACAAATACTACATCGGAGAAATTGATCCTTCGACGGTTCCTTCGAAGAAGATTTACATTCCATCACAACAAACCCAGTACAATCAAGATAAGACGCCCGAGTTTGTGATCAAGATCTTGCAGATACTGGTTCCCATATTTATTTTGGGTTTGGCATTTGCCGTTCGACACTACACCAAGAATGAATGA

mRNA sequence

TTTTGACTTCGTAATTAACTGCAAATCAAGGGCCATTTTGCCGGCCTCGTTTTTGTTCTTTCATTGTTACTATAAAACGATTTGTTTCCTCCGTTGCCAAATCGGGAACTAATCTGTAATCAGGCTTCGCCACAAACAGGATCCGATCAAGTCGTTGCCTCTTCAATCCTTTTTCATCTTGATATATGGCTTCCGATCCGAAGGTTCATCTGTTCGAAGAAGTGGCAAAGCATAACAAAACGAAGGATTGTTGGCTTGTAATCTCTGGAAAGGTTTATGATGTAACTCCTTTCATGGAGGATCACCCCGGTGGCGATGAAGTATTGTTGTCTGCAACGGGAAAAGATGCCTCGAACGATTTCGAGGATGTCGGGCACAGTGATTCAGCAAGAGAAATGATGGACAAATACTACATCGGAGAAATTGATCCTTCGACGGTTCCTTCGAAGAAGATTTACATTCCATCACAACAAACCCAGTACAATCAAGATAAGACGCCCGAGTTTGTGATCAAGATCTTGCAGATACTGGTTCCCATATTTATTTTGGGTTTGGCATTTGCCGTTCGACACTACACCAAGAATGAATGA

Coding sequence (CDS)

ATGGCTTCCGATCCGAAGGTTCATCTGTTCGAAGAAGTGGCAAAGCATAACAAAACGAAGGATTGTTGGCTTGTAATCTCTGGAAAGGTTTATGATGTAACTCCTTTCATGGAGGATCACCCCGGTGGCGATGAAGTATTGTTGTCTGCAACGGGAAAAGATGCCTCGAACGATTTCGAGGATGTCGGGCACAGTGATTCAGCAAGAGAAATGATGGACAAATACTACATCGGAGAAATTGATCCTTCGACGGTTCCTTCGAAGAAGATTTACATTCCATCACAACAAACCCAGTACAATCAAGATAAGACGCCCGAGTTTGTGATCAAGATCTTGCAGATACTGGTTCCCATATTTATTTTGGGTTTGGCATTTGCCGTTCGACACTACACCAAGAATGAATGA

Protein sequence

MASDPKVHLFEEVAKHNKTKDCWLVISGKVYDVTPFMEDHPGGDEVLLSATGKDASNDFEDVGHSDSAREMMDKYYIGEIDPSTVPSKKIYIPSQQTQYNQDKTPEFVIKILQILVPIFILGLAFAVRHYTKNE

Homology

[Close]

BLAST of Carg13998 vs. NCBI nr
Match: XP_022995583.1 (cytochrome b5-like [Cucurbita maxima] >XP_023532432.1 cytochrome b5-like [Cucurbita pepo subsp. pepo] >KAG6605804.1 Cytochrome b5 isoform E, partial [Cucurbita argyrosperma subsp. sororia] >KAG7035767.1 Cytochrome b5 isoform E [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 278.1 bits (710), Expect = 4.0e-71
Identity = 134/134 (100.00%), Postives = 134/134 (100.00%), Query Frame = 0


Query: 1   MASDPKVHLFEEVAKHNKTKDCWLVISGKVYDVTPFMEDHPGGDEVLLSATGKDASNDFE 60
           MASDPKVHLFEEVAKHNKTKDCWLVISGKVYDVTPFMEDHPGGDEVLLSATGKDASNDFE
Sbjct: 1   MASDPKVHLFEEVAKHNKTKDCWLVISGKVYDVTPFMEDHPGGDEVLLSATGKDASNDFE 60

Query: 61  DVGHSDSAREMMDKYYIGEIDPSTVPSKKIYIPSQQTQYNQDKTPEFVIKILQILVPIFI 120
           DVGHSDSAREMMDKYYIGEIDPSTVPSKKIYIPSQQTQYNQDKTPEFVIKILQILVPIFI
Sbjct: 61  DVGHSDSAREMMDKYYIGEIDPSTVPSKKIYIPSQQTQYNQDKTPEFVIKILQILVPIFI 120

Query: 121 LGLAFAVRHYTKNE 135
           LGLAFAVRHYTKNE
Sbjct: 121 LGLAFAVRHYTKNE 134

[Close]

BLAST of Carg13998 vs. NCBI nr
Match: XP_022958296.1 (cytochrome b5-like [Cucurbita moschata])

HSP 1 Score: 276.2 bits (705), Expect = 1.5e-70
Identity = 133/134 (99.25%), Postives = 134/134 (100.00%), Query Frame = 0


Query: 1   MASDPKVHLFEEVAKHNKTKDCWLVISGKVYDVTPFMEDHPGGDEVLLSATGKDASNDFE 60
           MASDPKVHLFEEVAKHNKTKDCWLVISGKVYDVTPFMEDHPGGDEVLLSATGKDASNDFE
Sbjct: 1   MASDPKVHLFEEVAKHNKTKDCWLVISGKVYDVTPFMEDHPGGDEVLLSATGKDASNDFE 60

Query: 61  DVGHSDSAREMMDKYYIGEIDPSTVPSKKIYIPSQQTQYNQDKTPEFVIKILQILVPIFI 120
           DVGHSDSAREMM+KYYIGEIDPSTVPSKKIYIPSQQTQYNQDKTPEFVIKILQILVPIFI
Sbjct: 61  DVGHSDSAREMMNKYYIGEIDPSTVPSKKIYIPSQQTQYNQDKTPEFVIKILQILVPIFI 120

Query: 121 LGLAFAVRHYTKNE 135
           LGLAFAVRHYTKNE
Sbjct: 121 LGLAFAVRHYTKNE 134

[Close]

BLAST of Carg13998 vs. NCBI nr
Match: XP_022137808.1 (cytochrome b5 [Momordica charantia])

HSP 1 Score: 269.2 bits (687), Expect = 1.9e-68
Identity = 128/134 (95.52%), Postives = 132/134 (98.51%), Query Frame = 0


Query: 1   MASDPKVHLFEEVAKHNKTKDCWLVISGKVYDVTPFMEDHPGGDEVLLSATGKDASNDFE 60
           MASDPKVHLFEEVAKHNKTKDCWLVISGKVYDVTPFMEDHPGGDEVLLSATGKDA+NDFE
Sbjct: 1   MASDPKVHLFEEVAKHNKTKDCWLVISGKVYDVTPFMEDHPGGDEVLLSATGKDATNDFE 60

Query: 61  DVGHSDSAREMMDKYYIGEIDPSTVPSKKIYIPSQQTQYNQDKTPEFVIKILQILVPIFI 120
           DVGHSDSAREMMDKYYIGEIDPSTVP K++YIP+QQTQYN DKTPEFVIKILQILVPIFI
Sbjct: 61  DVGHSDSAREMMDKYYIGEIDPSTVPLKRMYIPAQQTQYNSDKTPEFVIKILQILVPIFI 120

Query: 121 LGLAFAVRHYTKNE 135
           LGLAFAVRHYTKNE
Sbjct: 121 LGLAFAVRHYTKNE 134

[Close]

BLAST of Carg13998 vs. NCBI nr
Match: XP_038875650.1 (cytochrome b5 [Benincasa hispida])

HSP 1 Score: 267.7 bits (683), Expect = 5.4e-68
Identity = 128/134 (95.52%), Postives = 131/134 (97.76%), Query Frame = 0


Query: 1   MASDPKVHLFEEVAKHNKTKDCWLVISGKVYDVTPFMEDHPGGDEVLLSATGKDASNDFE 60
           MASDPKVHLFEEVAKHNKTKDCWLVISGKVYDVTPFMEDHPGGDEVLLSATGKDA+NDFE
Sbjct: 1   MASDPKVHLFEEVAKHNKTKDCWLVISGKVYDVTPFMEDHPGGDEVLLSATGKDATNDFE 60

Query: 61  DVGHSDSAREMMDKYYIGEIDPSTVPSKKIYIPSQQTQYNQDKTPEFVIKILQILVPIFI 120
           DVGHSD+AREMMDKYYIGEIDPSTVPSKKIYIPSQQTQ+N DKTPEFVIKILQ LVPI I
Sbjct: 61  DVGHSDAAREMMDKYYIGEIDPSTVPSKKIYIPSQQTQHNPDKTPEFVIKILQFLVPILI 120

Query: 121 LGLAFAVRHYTKNE 135
           LGLAFAVRHYTKNE
Sbjct: 121 LGLAFAVRHYTKNE 134

[Close]

BLAST of Carg13998 vs. NCBI nr
Match: XP_022922141.1 (cytochrome b5-like isoform X2 [Cucurbita moschata] >XP_022922142.1 cytochrome b5-like isoform X2 [Cucurbita moschata] >XP_023549751.1 cytochrome b5-like [Cucurbita pepo subsp. pepo] >KAG6579306.1 Cytochrome b5 isoform E, partial [Cucurbita argyrosperma subsp. sororia] >KAG7016804.1 Cytochrome b5 isoform E [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 266.9 bits (681), Expect = 9.2e-68
Identity = 128/134 (95.52%), Postives = 131/134 (97.76%), Query Frame = 0


Query: 1   MASDPKVHLFEEVAKHNKTKDCWLVISGKVYDVTPFMEDHPGGDEVLLSATGKDASNDFE 60
           MASDPKVHLFEEVAKHNKTKDCWLVISGKVYDVTPFMEDHPGGDEVLL+ATGKDA+NDFE
Sbjct: 1   MASDPKVHLFEEVAKHNKTKDCWLVISGKVYDVTPFMEDHPGGDEVLLAATGKDATNDFE 60

Query: 61  DVGHSDSAREMMDKYYIGEIDPSTVPSKKIYIPSQQTQYNQDKTPEFVIKILQILVPIFI 120
           DVGHSDSAREMMDKYYIGEIDPSTVP +KIYIPSQQTQYN DKT EFVIKILQILVPIFI
Sbjct: 61  DVGHSDSAREMMDKYYIGEIDPSTVPLRKIYIPSQQTQYNPDKTAEFVIKILQILVPIFI 120

Query: 121 LGLAFAVRHYTKNE 135
           LGLAFAVRHYTKNE
Sbjct: 121 LGLAFAVRHYTKNE 134

[Close]

BLAST of Carg13998 vs. ExPASy Swiss-Prot
Match: Q42342 (Cytochrome b5 isoform E OS=Arabidopsis thaliana OX=3702 GN=CYTB5-E PE=1 SV=2)

HSP 1 Score: 225.3 bits (573), Expect = 4.0e-58
Identity = 104/134 (77.61%), Postives = 119/134 (88.81%), Query Frame = 0


Query: 1   MASDPKVHLFEEVAKHNKTKDCWLVISGKVYDVTPFMEDHPGGDEVLLSATGKDASNDFE 60
           M+SD KV  FEEV+KHNKTKDCWL+ISGKVYDVTPFM+DHPGGDEVLLS+TGKDA+NDFE
Sbjct: 1   MSSDRKVLSFEEVSKHNKTKDCWLIISGKVYDVTPFMDDHPGGDEVLLSSTGKDATNDFE 60

Query: 61  DVGHSDSAREMMDKYYIGEIDPSTVPSKKIYIPSQQTQYNQDKTPEFVIKILQILVPIFI 120
           DVGHSD+AR+MMDKY+IGEID S+VP+ + Y+  QQ  YNQDKTPEF+IKILQ LVPI I
Sbjct: 61  DVGHSDTARDMMDKYFIGEIDSSSVPATRTYVAPQQPAYNQDKTPEFIIKILQFLVPILI 120

Query: 121 LGLAFAVRHYTKNE 135
           LGLA  VRHYTK +
Sbjct: 121 LGLALVVRHYTKKD 134

[Close]

BLAST of Carg13998 vs. ExPASy Swiss-Prot
Match: P40934 (Cytochrome b5 OS=Brassica oleracea var. botrytis OX=3715 GN=CYB5 PE=1 SV=1)

HSP 1 Score: 219.2 bits (557), Expect = 2.9e-56
Identity = 103/134 (76.87%), Postives = 117/134 (87.31%), Query Frame = 0


Query: 1   MASDPKVHLFEEVAKHNKTKDCWLVISGKVYDVTPFMEDHPGGDEVLLSATGKDASNDFE 60
           MAS+ KV  FEEV++HNKTKDCWL+ISGKVYDVTPFM+DHPGGDEVLLS+TGKDA+NDFE
Sbjct: 1   MASEKKVLGFEEVSQHNKTKDCWLIISGKVYDVTPFMDDHPGGDEVLLSSTGKDATNDFE 60

Query: 61  DVGHSDSAREMMDKYYIGEIDPSTVPSKKIYIPSQQTQYNQDKTPEFVIKILQILVPIFI 120
           DVGHSD+AR+MM+KYYIGEID STVP+ + Y+   Q  YNQDKTPEF+IKILQ LVPI I
Sbjct: 61  DVGHSDTARDMMEKYYIGEIDSSTVPATRTYVAPVQPAYNQDKTPEFMIKILQFLVPILI 120

Query: 121 LGLAFAVRHYTKNE 135
           LGLA  VR YTK E
Sbjct: 121 LGLALVVRQYTKKE 134

[Close]

BLAST of Carg13998 vs. ExPASy Swiss-Prot
Match: P49100 (Cytochrome b5 OS=Oryza sativa subsp. japonica OX=39947 GN=Os05g0108800 PE=2 SV=2)

HSP 1 Score: 196.4 bits (498), Expect = 2.0e-49
Identity = 88/129 (68.22%), Postives = 110/129 (85.27%), Query Frame = 0


Query: 6   KVHLFEEVAKHNKTKDCWLVISGKVYDVTPFMEDHPGGDEVLLSATGKDASNDFEDVGHS 65
           KV+  EEVAKHN   DCWL+I GKVY+V+ F+EDHPGGD+VLLS+TGKDA++DFEDVGHS
Sbjct: 7   KVYTLEEVAKHNSKDDCWLIIGGKVYNVSKFLEDHPGGDDVLLSSTGKDATDDFEDVGHS 66

Query: 66  DSAREMMDKYYIGEIDPSTVPSKKIYIPSQQTQYNQDKTPEFVIKILQILVPIFILGLAF 125
            +AR MMD+YY+G+ID ST+P++  Y+P +Q  YNQDKTPEF+IKILQ LVP+ ILGLA 
Sbjct: 67  TTARAMMDEYYVGDIDTSTIPARTKYVPPKQPHYNQDKTPEFIIKILQFLVPLAILGLAV 126

Query: 126 AVRHYTKNE 135
           A+R YTK+E
Sbjct: 127 AIRIYTKSE 135

[Close]

BLAST of Carg13998 vs. ExPASy Swiss-Prot
Match: P49098 (Cytochrome b5 OS=Nicotiana tabacum OX=4097 PE=2 SV=1)

HSP 1 Score: 195.3 bits (495), Expect = 4.5e-49
Identity = 88/132 (66.67%), Postives = 107/132 (81.06%), Query Frame = 0


Query: 1   MASDPKVHLFEEVAKHNKTKDCWLVISGKVYDVTPFMEDHPGGDEVLLSATGKDASNDFE 60
           M  + KV    EV++HN  KDCWLVISGKVYDVT F++DHPGGDEVLLSATGKDA++DFE
Sbjct: 1   MGGETKVFTLAEVSQHNNAKDCWLVISGKVYDVTKFLDDHPGGDEVLLSATGKDATDDFE 60

Query: 61  DVGHSDSAREMMDKYYIGEIDPSTVPSKKIYIPSQQTQYNQDKTPEFVIKILQILVPIFI 120
           DVGHS SAR M+D+YY+G+ID +T+P+K  Y P  Q  YNQDKT EFV+K+LQ LVP+ I
Sbjct: 61  DVGHSSSARAMLDEYYVGDIDSATIPTKTKYTPPNQPHYNQDKTSEFVVKLLQFLVPLII 120

Query: 121 LGLAFAVRHYTK 133
           LG+AF +R YTK
Sbjct: 121 LGVAFGIRFYTK 132

[Close]

BLAST of Carg13998 vs. ExPASy Swiss-Prot
Match: O48845 (Cytochrome b5 isoform B OS=Arabidopsis thaliana OX=3702 GN=CYTB5-B PE=1 SV=1)

HSP 1 Score: 192.6 bits (488), Expect = 2.9e-48
Identity = 85/133 (63.91%), Postives = 110/133 (82.71%), Query Frame = 0


Query: 1   MASDPKVHLFEEVAKHNKTKDCWLVISGKVYDVTPFMEDHPGGDEVLLSATGKDASNDFE 60
           M  + K+    EV++HN+  DCW+VI+GKVY+VT F+EDHPGGD+VLLS+TGKDA++DFE
Sbjct: 1   MGDEAKIFTLSEVSEHNQAHDCWIVINGKVYNVTKFLEDHPGGDDVLLSSTGKDATDDFE 60

Query: 61  DVGHSDSAREMMDKYYIGEIDPSTVPSKKIYIPSQQTQYNQDKTPEFVIKILQILVPIFI 120
           DVGHS+SAREMM++YY+GEIDP+T+P K  Y P +Q  YNQDKT EF+IK+LQ LVP+ I
Sbjct: 61  DVGHSESAREMMEQYYVGEIDPTTIPKKVKYTPPKQPHYNQDKTSEFIIKLLQFLVPLAI 120

Query: 121 LGLAFAVRHYTKN 134
           LGLA  +R YTK+
Sbjct: 121 LGLAVGIRIYTKS 133

[Close]

BLAST of Carg13998 vs. ExPASy TrEMBL
Match: A0A6J1K8E3 (cytochrome b5-like OS=Cucurbita maxima OX=3661 GN=LOC111491071 PE=3 SV=1)

HSP 1 Score: 278.1 bits (710), Expect = 1.9e-71
Identity = 134/134 (100.00%), Postives = 134/134 (100.00%), Query Frame = 0


Query: 1   MASDPKVHLFEEVAKHNKTKDCWLVISGKVYDVTPFMEDHPGGDEVLLSATGKDASNDFE 60
           MASDPKVHLFEEVAKHNKTKDCWLVISGKVYDVTPFMEDHPGGDEVLLSATGKDASNDFE
Sbjct: 1   MASDPKVHLFEEVAKHNKTKDCWLVISGKVYDVTPFMEDHPGGDEVLLSATGKDASNDFE 60

Query: 61  DVGHSDSAREMMDKYYIGEIDPSTVPSKKIYIPSQQTQYNQDKTPEFVIKILQILVPIFI 120
           DVGHSDSAREMMDKYYIGEIDPSTVPSKKIYIPSQQTQYNQDKTPEFVIKILQILVPIFI
Sbjct: 61  DVGHSDSAREMMDKYYIGEIDPSTVPSKKIYIPSQQTQYNQDKTPEFVIKILQILVPIFI 120

Query: 121 LGLAFAVRHYTKNE 135
           LGLAFAVRHYTKNE
Sbjct: 121 LGLAFAVRHYTKNE 134

[Close]

BLAST of Carg13998 vs. ExPASy TrEMBL
Match: A0A6J1H1Q1 (cytochrome b5-like OS=Cucurbita moschata OX=3662 GN=LOC111459560 PE=3 SV=1)

HSP 1 Score: 276.2 bits (705), Expect = 7.4e-71
Identity = 133/134 (99.25%), Postives = 134/134 (100.00%), Query Frame = 0


Query: 1   MASDPKVHLFEEVAKHNKTKDCWLVISGKVYDVTPFMEDHPGGDEVLLSATGKDASNDFE 60
           MASDPKVHLFEEVAKHNKTKDCWLVISGKVYDVTPFMEDHPGGDEVLLSATGKDASNDFE
Sbjct: 1   MASDPKVHLFEEVAKHNKTKDCWLVISGKVYDVTPFMEDHPGGDEVLLSATGKDASNDFE 60

Query: 61  DVGHSDSAREMMDKYYIGEIDPSTVPSKKIYIPSQQTQYNQDKTPEFVIKILQILVPIFI 120
           DVGHSDSAREMM+KYYIGEIDPSTVPSKKIYIPSQQTQYNQDKTPEFVIKILQILVPIFI
Sbjct: 61  DVGHSDSAREMMNKYYIGEIDPSTVPSKKIYIPSQQTQYNQDKTPEFVIKILQILVPIFI 120

Query: 121 LGLAFAVRHYTKNE 135
           LGLAFAVRHYTKNE
Sbjct: 121 LGLAFAVRHYTKNE 134

[Close]

BLAST of Carg13998 vs. ExPASy TrEMBL
Match: A0A6J1CBE4 (cytochrome b5 OS=Momordica charantia OX=3673 GN=LOC111009154 PE=3 SV=1)

HSP 1 Score: 269.2 bits (687), Expect = 9.0e-69
Identity = 128/134 (95.52%), Postives = 132/134 (98.51%), Query Frame = 0


Query: 1   MASDPKVHLFEEVAKHNKTKDCWLVISGKVYDVTPFMEDHPGGDEVLLSATGKDASNDFE 60
           MASDPKVHLFEEVAKHNKTKDCWLVISGKVYDVTPFMEDHPGGDEVLLSATGKDA+NDFE
Sbjct: 1   MASDPKVHLFEEVAKHNKTKDCWLVISGKVYDVTPFMEDHPGGDEVLLSATGKDATNDFE 60

Query: 61  DVGHSDSAREMMDKYYIGEIDPSTVPSKKIYIPSQQTQYNQDKTPEFVIKILQILVPIFI 120
           DVGHSDSAREMMDKYYIGEIDPSTVP K++YIP+QQTQYN DKTPEFVIKILQILVPIFI
Sbjct: 61  DVGHSDSAREMMDKYYIGEIDPSTVPLKRMYIPAQQTQYNSDKTPEFVIKILQILVPIFI 120

Query: 121 LGLAFAVRHYTKNE 135
           LGLAFAVRHYTKNE
Sbjct: 121 LGLAFAVRHYTKNE 134

[Close]

BLAST of Carg13998 vs. ExPASy TrEMBL
Match: A0A6J1E3B3 (cytochrome b5-like isoform X2 OS=Cucurbita moschata OX=3662 GN=LOC111430208 PE=3 SV=1)

HSP 1 Score: 266.9 bits (681), Expect = 4.5e-68
Identity = 128/134 (95.52%), Postives = 131/134 (97.76%), Query Frame = 0


Query: 1   MASDPKVHLFEEVAKHNKTKDCWLVISGKVYDVTPFMEDHPGGDEVLLSATGKDASNDFE 60
           MASDPKVHLFEEVAKHNKTKDCWLVISGKVYDVTPFMEDHPGGDEVLL+ATGKDA+NDFE
Sbjct: 1   MASDPKVHLFEEVAKHNKTKDCWLVISGKVYDVTPFMEDHPGGDEVLLAATGKDATNDFE 60

Query: 61  DVGHSDSAREMMDKYYIGEIDPSTVPSKKIYIPSQQTQYNQDKTPEFVIKILQILVPIFI 120
           DVGHSDSAREMMDKYYIGEIDPSTVP +KIYIPSQQTQYN DKT EFVIKILQILVPIFI
Sbjct: 61  DVGHSDSAREMMDKYYIGEIDPSTVPLRKIYIPSQQTQYNPDKTAEFVIKILQILVPIFI 120

Query: 121 LGLAFAVRHYTKNE 135
           LGLAFAVRHYTKNE
Sbjct: 121 LGLAFAVRHYTKNE 134

[Close]

BLAST of Carg13998 vs. ExPASy TrEMBL
Match: A0A0A0KN46 (Cytochrome b5 heme-binding domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_5G139160 PE=3 SV=1)

HSP 1 Score: 263.5 bits (672), Expect = 4.9e-67
Identity = 126/134 (94.03%), Postives = 130/134 (97.01%), Query Frame = 0


Query: 1   MASDPKVHLFEEVAKHNKTKDCWLVISGKVYDVTPFMEDHPGGDEVLLSATGKDASNDFE 60
           MASDPKVHLFEEVAKHNKTKDCWLVISGKVYDVTPFMEDHPGGDEVLLSATGKDA+NDFE
Sbjct: 1   MASDPKVHLFEEVAKHNKTKDCWLVISGKVYDVTPFMEDHPGGDEVLLSATGKDATNDFE 60

Query: 61  DVGHSDSAREMMDKYYIGEIDPSTVPSKKIYIPSQQTQYNQDKTPEFVIKILQILVPIFI 120
           DVGHSDSAREMMDKYYIGEIDPSTVP KKI+IPSQQ+Q+N DKTPEFVIKILQ LVPI I
Sbjct: 61  DVGHSDSAREMMDKYYIGEIDPSTVPLKKIFIPSQQSQHNPDKTPEFVIKILQFLVPILI 120

Query: 121 LGLAFAVRHYTKNE 135
           LGLAFAVRHYTKNE
Sbjct: 121 LGLAFAVRHYTKNE 134

[Close]

BLAST of Carg13998 vs. TAIR 10
Match: AT5G53560.1 (cytochrome B5 isoform E )

HSP 1 Score: 225.3 bits (573), Expect = 2.9e-59
Identity = 104/134 (77.61%), Postives = 119/134 (88.81%), Query Frame = 0


Query: 1   MASDPKVHLFEEVAKHNKTKDCWLVISGKVYDVTPFMEDHPGGDEVLLSATGKDASNDFE 60
           M+SD KV  FEEV+KHNKTKDCWL+ISGKVYDVTPFM+DHPGGDEVLLS+TGKDA+NDFE
Sbjct: 1   MSSDRKVLSFEEVSKHNKTKDCWLIISGKVYDVTPFMDDHPGGDEVLLSSTGKDATNDFE 60

Query: 61  DVGHSDSAREMMDKYYIGEIDPSTVPSKKIYIPSQQTQYNQDKTPEFVIKILQILVPIFI 120
           DVGHSD+AR+MMDKY+IGEID S+VP+ + Y+  QQ  YNQDKTPEF+IKILQ LVPI I
Sbjct: 61  DVGHSDTARDMMDKYFIGEIDSSSVPATRTYVAPQQPAYNQDKTPEFIIKILQFLVPILI 120

Query: 121 LGLAFAVRHYTKNE 135
           LGLA  VRHYTK +
Sbjct: 121 LGLALVVRHYTKKD 134

[Close]

BLAST of Carg13998 vs. TAIR 10
Match: AT2G32720.1 (cytochrome B5 isoform B )

HSP 1 Score: 192.6 bits (488), Expect = 2.1e-49
Identity = 85/133 (63.91%), Postives = 110/133 (82.71%), Query Frame = 0


Query: 1   MASDPKVHLFEEVAKHNKTKDCWLVISGKVYDVTPFMEDHPGGDEVLLSATGKDASNDFE 60
           M  + K+    EV++HN+  DCW+VI+GKVY+VT F+EDHPGGD+VLLS+TGKDA++DFE
Sbjct: 1   MGDEAKIFTLSEVSEHNQAHDCWIVINGKVYNVTKFLEDHPGGDDVLLSSTGKDATDDFE 60

Query: 61  DVGHSDSAREMMDKYYIGEIDPSTVPSKKIYIPSQQTQYNQDKTPEFVIKILQILVPIFI 120
           DVGHS+SAREMM++YY+GEIDP+T+P K  Y P +Q  YNQDKT EF+IK+LQ LVP+ I
Sbjct: 61  DVGHSESAREMMEQYYVGEIDPTTIPKKVKYTPPKQPHYNQDKTSEFIIKLLQFLVPLAI 120

Query: 121 LGLAFAVRHYTKN 134
           LGLA  +R YTK+
Sbjct: 121 LGLAVGIRIYTKS 133

[Close]

BLAST of Carg13998 vs. TAIR 10
Match: AT5G48810.1 (cytochrome B5 isoform D )

HSP 1 Score: 177.2 bits (448), Expect = 9.0e-45
Identity = 79/136 (58.09%), Postives = 107/136 (78.68%), Query Frame = 0


Query: 1   MASDPKVHLFEEVAKHNKTKDCWLVISGKVYDVTPFMEDHPGGDEVLLSATGKDASNDFE 60
           M  D KV    EV++H+  KDCW+VI GKVYDVT F++DHPGGDEV+L++TGKDA++DFE
Sbjct: 1   MGGDGKVFTLSEVSQHSSAKDCWIVIDGKVYDVTKFLDDHPGGDEVILTSTGKDATDDFE 60

Query: 61  DVGHSDSAREMMDKYYIGEIDPSTVPSKKIYIPSQQTQ--YNQDKTPEFVIKILQILVPI 120
           DVGHS +A+ M+D+YY+G+ID +TVP K  ++P   T+    QDK+ +FVIK+LQ LVP+
Sbjct: 61  DVGHSSTAKAMLDEYYVGDIDTATVPVKAKFVPPTSTKAVATQDKSSDFVIKLLQFLVPL 120

Query: 121 FILGLAFAVRHYTKNE 135
            ILGLAF +R+YTK +
Sbjct: 121 LILGLAFGIRYYTKTK 136

[Close]

BLAST of Carg13998 vs. TAIR 10
Match: AT2G46650.1 (cytochrome B5 isoform C )

HSP 1 Score: 136.0 bits (341), Expect = 2.3e-32
Identity = 66/123 (53.66%), Postives = 87/123 (70.73%), Query Frame = 0


Query: 10  FEEVAKHNKTKDCWLVISGKVYDVTPFMEDHPGGDEVLLSATGKDASNDFEDVGHSDSAR 69
           F +VAKH    DCW++I GKVYD++ FM++HPGGD VLL+ TGKDAS DFEDV HS  A+
Sbjct: 7   FHDVAKHKCKNDCWILIHGKVYDISTFMDEHPGGDNVLLAVTGKDASIDFEDVNHSKDAK 66

Query: 70  EMMDKYYIGEIDPSTVPSKKIYIPSQQTQYNQDKT--PEFVIKILQILVPIFILGLAFAV 129
           E+M KY IG++D STVP  + YIP  + +    +T   E   K+L  L+P+ ILG+AFA+
Sbjct: 67  ELMKKYCIGDVDQSTVPVTQQYIPPWEKESTAAETTKEESGKKLLIYLIPLLILGVAFAL 126

Query: 130 RHY 131
           R Y
Sbjct: 127 RFY 129

[Close]

BLAST of Carg13998 vs. TAIR 10
Match: AT1G26340.1 (cytochrome B5 isoform A )

HSP 1 Score: 125.6 bits (314), Expect = 3.1e-29
Identity = 61/127 (48.03%), Postives = 89/127 (70.08%), Query Frame = 0


Query: 6   KVHLFEEVAKHNKTKDCWLVISGKVYDVTPFMEDHPGGDEVLLSATGKDASNDFEDVGHS 65
           K++  EE A HNK  DCW+VI GKVYDV+ +M++HPGGD+VLL+  GKDA++DFED GHS
Sbjct: 6   KLYSMEEAATHNKQDDCWVVIDGKVYDVSSYMDEHPGGDDVLLAVAGKDATDDFEDAGHS 65

Query: 66  DSAREMMDKYYIGEIDPSTVPSKKIYIPSQQTQYNQDKTPEFVIKILQI-----LVPIFI 125
             ARE+M+KY+IGE+D S++P     IP  +  Y +D+  + V K+  +     +VP+ I
Sbjct: 66  KDARELMEKYFIGELDESSLPE----IPELKI-YKKDQPQDSVQKLFDLTKQYWVVPVSI 125

Query: 126 LGLAFAV 128
           + ++ AV
Sbjct: 126 ITISVAV 127

The following BLAST results are available for this feature:

BLAST of Carg13998 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_022995583.1	4.0e-71	100.00	cytochrome b5-like [Cucurbita maxima] >XP_023532432.1 cytochrome b5-like [Cucurb...	[more]
XP_022958296.1	1.5e-70	99.25	cytochrome b5-like [Cucurbita moschata]	[more]
XP_022137808.1	1.9e-68	95.52	cytochrome b5 [Momordica charantia]	[more]
XP_038875650.1	5.4e-68	95.52	cytochrome b5 [Benincasa hispida]	[more]
XP_022922141.1	9.2e-68	95.52	cytochrome b5-like isoform X2 [Cucurbita moschata] >XP_022922142.1 cytochrome b5...	[more]

BLAST of Carg13998 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q42342	4.0e-58	77.61	Cytochrome b5 isoform E OS=Arabidopsis thaliana OX=3702 GN=CYTB5-E PE=1 SV=2	[more]
P40934	2.9e-56	76.87	Cytochrome b5 OS=Brassica oleracea var. botrytis OX=3715 GN=CYB5 PE=1 SV=1	[more]
P49100	2.0e-49	68.22	Cytochrome b5 OS=Oryza sativa subsp. japonica OX=39947 GN=Os05g0108800 PE=2 SV=2	[more]
P49098	4.5e-49	66.67	Cytochrome b5 OS=Nicotiana tabacum OX=4097 PE=2 SV=1	[more]
O48845	2.9e-48	63.91	Cytochrome b5 isoform B OS=Arabidopsis thaliana OX=3702 GN=CYTB5-B PE=1 SV=1	[more]

BLAST of Carg13998 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1K8E3	1.9e-71	100.00	cytochrome b5-like OS=Cucurbita maxima OX=3661 GN=LOC111491071 PE=3 SV=1	[more]
A0A6J1H1Q1	7.4e-71	99.25	cytochrome b5-like OS=Cucurbita moschata OX=3662 GN=LOC111459560 PE=3 SV=1	[more]
A0A6J1CBE4	9.0e-69	95.52	cytochrome b5 OS=Momordica charantia OX=3673 GN=LOC111009154 PE=3 SV=1	[more]
A0A6J1E3B3	4.5e-68	95.52	cytochrome b5-like isoform X2 OS=Cucurbita moschata OX=3662 GN=LOC111430208 PE=3...	[more]
A0A0A0KN46	4.9e-67	94.03	Cytochrome b5 heme-binding domain-containing protein OS=Cucumis sativus OX=3659 ...	[more]

BLAST of Carg13998 vs. TAIR 10


Match Name	E-value	Identity	Description
AT5G53560.1	2.9e-59	77.61	cytochrome B5 isoform E	[more]
AT2G32720.1	2.1e-49	63.91	cytochrome B5 isoform B	[more]
AT5G48810.1	9.0e-45	58.09	cytochrome B5 isoform D	[more]
AT2G46650.1	2.3e-32	53.66	cytochrome B5 isoform C	[more]
AT1G26340.1	3.1e-29	48.03	cytochrome B5 isoform A	[more]

InterPro

Analysis Name: InterPro Annotations of Silver-seed gourd (SMH-JMG-627) v2
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR001199	Cytochrome b5-like heme/steroid binding domain	PRINTS	PR00363	CYTOCHROMEB5	coord: 30..40 score: 60.74 coord: 40..54 score: 54.65 coord: 55..62 score: 35.8 coord: 68..80 score: 44.99
IPR001199	Cytochrome b5-like heme/steroid binding domain	SMART	SM01117	Cyt_b5_2	coord: 8..81 e-value: 3.6E-30 score: 116.2
IPR001199	Cytochrome b5-like heme/steroid binding domain	PFAM	PF00173	Cyt-b5	coord: 11..80 e-value: 1.0E-26 score: 92.8
IPR001199	Cytochrome b5-like heme/steroid binding domain	PROSITE	PS50255	CYTOCHROME_B5_2	coord: 5..81 score: 29.183647
IPR036400	Cytochrome b5-like heme/steroid binding domain superfamily	GENE3D	3.10.120.10		coord: 1..87 e-value: 3.9E-36 score: 124.9
IPR036400	Cytochrome b5-like heme/steroid binding domain superfamily	SUPERFAMILY	55856	Cytochrome b5-like heme/steroid binding domain	coord: 4..86
None	No IPR available	PANTHER	PTHR19359	CYTOCHROME B5	coord: 1..132
None	No IPR available	PANTHER	PTHR19359:SF103	CYTOCHROME B5-LIKE	coord: 1..132
IPR018506	Cytochrome b5, heme-binding site	PROSITE	PS00191	CYTOCHROME_B5_1	coord: 36..43

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Carg13998-RA	Carg13998-RA	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0035672	oligopeptide transmembrane transport
cellular_component	GO:0016021	integral component of membrane
cellular_component	GO:0043231	intracellular membrane-bounded organelle
molecular_function	GO:0020037	heme binding
molecular_function	GO:0046872	metal ion binding
molecular_function	GO:0035673	oligopeptide transmembrane transporter activity