Carg13978 (gene) Silver-seed gourd (SMH-JMG-627) v2

Overview


Name	Carg13978
Type	gene
Organism	Cucurbita argyrosperma subsp. argyrosperma cv. SMH-JMG-627 (Silver-seed gourd (SMH-JMG-627) v2)
Description	Mediator of RNA polymerase II transcription subunit 21
Location	Carg_Chr02: 6781417 .. 6784937 (+)
RNA-Seq Expression	Carg13978
Synteny	Carg13978

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRCDSpolypeptidethree_prime_UTR

Hold the cursor over a type above to highlight its positions in the sequence below.

ATTTTACTCTCTCGTGCTCCGCCCGGTCACGTCGTCTCCCCCTCCCTTTCAGCCGTTCAGTGTCGCCACCGCTTCTGTCCGGTCCTTGTCGCCGCCGCCTCTCTCTGGTCAGTGCTGCCGCCGCAGCCTCCCTCAGGTTTGCACTGATTTTATACTCTTCTGATGTTACCGATATTCTCCCTTTCTTCATCTCTGCCTTTTTTCTCGCTTGAAAAACTCAAATTGGGAATTGTATAAGGAAAAAATGGAGTTGTTTTGATTTTTTGAAATGACAATAGACGAACTGCGGCGTGCTTTGCCTTTAGATTCGTTGAGTATGTCTACTGAGGCTTGATCGTCTGTGCATCTGTCTTGTTTCTGCGAGAATGAAATGTTCAGCGTAATTATTTCTTTGTGTATGAGAGAATCAGTAAGTTATTGTATTATCGAATTCTTTTTTAATCCCTAGCTTGGCGAAGCTATTTTGATTGACCAACAACTTTCAACCATACTTGTAGTTGTAGATTTGGAGACTATAGTTGGCAGATAACAATGAATCTTTTTTTAAAGTTGAAATTCTCCTCCCTCACATCCATCAATCACCCAACATACTTTTAATTTACTGGAAATAAAAGCCATCTATATGCTTTGTACTTCATGTAATTGCCATAAAAAGTTTTAGAAAATGCATAGAGTAAAATAGGAAGGGAACTAACGATTTTCCTATATAGTATAGATTATTCCGGTATTGGTACGGCACTGATACTTTTTTCGTTGGCATAATCCAACTTGGCTGCTCTTCCAAGGTTTTCGGTGAGCTCAGTTTTGTGAGAATTGGAATTGGTTTATCCATTAATGATAAACAGATCTAACATCTTACAATCACGTCATTACAATTTTGATTTCGATCCTCTAATACTGTTTCCTTATATTTGACGTGATTTCATCATTTACTTGAGCAACCAAACACAAGATCCAGATTAGAACCTGCGAACCTATTGACTCACTCCTAGTCTCTTCAATTGTAATAAATTAAATTGAGAAACTATGGATCGAGGTATTTCATTCAGTGTTTCTTTTATTGAATTGGTTGTTTTTCTTCCACAACGTTGTCTCTCAGCAATTAAAGTCGGATGTGAAGTCTTCTTACGAGTATAAACTTTCCTTACCAAGTTTTTGATGCTATTTCAGATTTTTTTTTTTTTTTTTGGATTATATTGGAAAAAATAGGTTGGACTCCCACTCAGTCAGTTCCTAAATGTGATATGTTGATTTTAATTGTCTTGATATGTTTATTGGTTCTCATCATTTTCTGGACAATTCTTCTGGGGGATAAGGTTCGAAATGGACATTATTTCCCAACTACAGGAACAAGTCAATACAATTGCTGCTTTAGCTTTCAATACTTTTGGGACATTGCAGAGAGATGCTCCTCCAGTTCGTTTATCTCCTAATTACCCCGAACCTCCGCCTCACGAACCTACAGAGGACAGTGCAAACTTCGCAGAGCAGCCCAAGCTAATGAGTGCCGCTTTGGTGAAAGCCGCTAAGCAGGTGTGATTTTTATTTTGCAGGATTGTGTTTTCCGTTGTTCATGCTAAGTGTGTAGTTCGTTGGTTCTTGTACTTTTGGGGTGGAAAATATGAATGTGATGCATATGCCTCTTGTTATTTACTTTTTCATGGGTCGAAACATCAAGAAAGTATTTTCTTCTTCCTTTTGGGGAGATGTTAGATTGCCATTTTTATTTTTTTTTTCCAAATGTCAGTATCCTAGATTGATTAACTTTACATGCTTTCTATCTACTGGATAAGTAATTGAGAAACAACTGTCTACTCGAAGCTCCTTTGTGATGAGATTGAAAGAGATGCATTTCATTCACCCTCAAATGTTCGTTTGTTAGATCTAGTTAATGCCAAGTCTGAACTTGTGTTGTCTGAGGATGAAACAGGACTGTGATTTCCTGCTTTACAGCCGCTCATAACTTCTATTGCGTTTTTTATGGGGTAGTAATATATCAAAGCAACGGATCTCCTGCTACTTGTTTGAGATGCATACTAATTCTAATGCTATGATTTATTGTATATCCATCAAAGGAAGCAAATTTGGTATTGGAAACTTCAATTTACTTTTGCCTTCCAACCTCAGATACTACTTGGGCAAATATGGTGTTTTAGATCAGCAATAAGCCAAAGTCTTACGGAAAACTTTTCTAACTATTCTTTGCTTTTAACTGTATATATGTTTTAGCCCCACTTTTAAGAGCTATTAAGCGTGTTCATATTTTTGGAATCTGCTATTCAACCCTGTATTTGACTAAAATCTGGATCTTTTTTTTATTTAGAAAATTTTGCTGAGAGATTTTGATTTTCGTAAGCAATGGAGTTGAAAGTAGCAATGAATTAAGGTTTTTTCTCTTCAAAAGTTTTTTCCATGGAATATGTAGAATTTATAAAATCGACGAGTCTGTCCACATTACGTTTGAGAAAATCCCAGTGTAGTTTTTGGGTAATAGAAATGTTTGGATGTTTTCCAGTTCTGGTAGCATTTTAGTGCCTTCTAGATGTGATTCTACAAGGAGCCAGATTGTTCCATTCCTTCTCTCATTTTCTTTTCCTTTTTTACTCTCTCCTCTCAAACTGGTATACTATTAGCTTTCTGTACAAAAGAAAGGGTTGAGACCATGCTTCATTTATATATAATCCAATTGAACATATGTACCGTTCTTGAAACTATTGTTGTTATTATCTTTTGGCACAGTTTGATGTATTAGTTGCAGCATTGCCTCTATCTGATGGAGGTGAAGAAGAGCAGTTGAAAAGGATTGCAGAACTACAGGTATAATAAGCCTTCTCATATTCTAATGTTGATATAAGACCAAGAATTCCTGTATTGTTTAGTTCTCATTACATTCGAAACTTGTTATCCAGGCCGAGAACGATGCTGTAGGTCAAGAACTTCAAAAGCAGCTAGAAGCAGCAGGTATTATCTCGTTTTCTTCATTAACCTGTGTATATTTACAACATGCCCAGTTTCAAATTATGGGCCCACCAAGAATGACTTCCTATTGGAAATTTTACTGTATGGTTTCGTTTGGGGTTTGACCAACTCGTCATAAGCGCGAAATTTCTCGTGTTCTCTCCGTTCTATTGAACATACTACTGATATTTGGCCACCGCTACACAATCTAACATTCTTAATGGAATTGGGAATTGTTTGTTGCAGAGAAGGAATTAAAACAGGTTCAGGAGCTTTTCAGCCAAGTTGCAGATAATTGCTTGAACTTGAAGAAAGCAGACTGAAAGATTTCAAGGTTATAATAGTAGATCAATCAAATGCATTCATTCCTTTCTTCCCAATTCAATGCAACTTAGAACAGGTGTGTTAATTTTCTGCGCCACTTTGTCCATTTTCTCATCGTTTTTTTAGCTCAACTCGGTAGAAGATTCATGATGCCTAACCCCAAATTGCCTCTCTTTAGCTTCTAGCGGATGTTCAAAGTTGTCAAAGTAATTGCAAGTTAGCAATATATTATGTATTTG

mRNA sequence

ATTTTACTCTCTCGTGCTCCGCCCGGTCACGTCGTCTCCCCCTCCCTTTCAGCCGTTCAGTGTCGCCACCGCTTCTGTCCGGTCCTTGTCGCCGCCGCCTCTCTCTGGTCAGTGCTGCCGCCGCAGCCTCCCTCAGGTTCGAAATGGACATTATTTCCCAACTACAGGAACAAGTCAATACAATTGCTGCTTTAGCTTTCAATACTTTTGGGACATTGCAGAGAGATGCTCCTCCAGTTCGTTTATCTCCTAATTACCCCGAACCTCCGCCTCACGAACCTACAGAGGACAGTGCAAACTTCGCAGAGCAGCCCAAGCTAATGAGTGCCGCTTTGGTGAAAGCCGCTAAGCAGTTTGATGTATTAGTTGCAGCATTGCCTCTATCTGATGGAGGTGAAGAAGAGCAGTTGAAAAGGATTGCAGAACTACAGGCCGAGAACGATGCTGTAGGTCAAGAACTTCAAAAGCAGCTAGAAGCAGCAGAGAAGGAATTAAAACAGGTTCAGGAGCTTTTCAGCCAAGTTGCAGATAATTGCTTGAACTTGAAGAAAGCAGACTGAAAGATTTCAAGGTTATAATAGTAGATCAATCAAATGCATTCATTCCTTTCTTCCCAATTCAATGCAACTTAGAACAGGTGTGTTAATTTTCTGCGCCACTTTGTCCATTTTCTCATCGTTTTTTTAGCTCAACTCGGTAGAAGATTCATGATGCCTAACCCCAAATTGCCTCTCTTTAGCTTCTAGCGGATGTTCAAAGTTGTCAAAGTAATTGCAAGTTAGCAATATATTATGTATTTG

Coding sequence (CDS)

ATGGACATTATTTCCCAACTACAGGAACAAGTCAATACAATTGCTGCTTTAGCTTTCAATACTTTTGGGACATTGCAGAGAGATGCTCCTCCAGTTCGTTTATCTCCTAATTACCCCGAACCTCCGCCTCACGAACCTACAGAGGACAGTGCAAACTTCGCAGAGCAGCCCAAGCTAATGAGTGCCGCTTTGGTGAAAGCCGCTAAGCAGTTTGATGTATTAGTTGCAGCATTGCCTCTATCTGATGGAGGTGAAGAAGAGCAGTTGAAAAGGATTGCAGAACTACAGGCCGAGAACGATGCTGTAGGTCAAGAACTTCAAAAGCAGCTAGAAGCAGCAGAGAAGGAATTAAAACAGGTTCAGGAGCTTTTCAGCCAAGTTGCAGATAATTGCTTGAACTTGAAGAAAGCAGACTGA

Protein sequence

MDIISQLQEQVNTIAALAFNTFGTLQRDAPPVRLSPNYPEPPPHEPTEDSANFAEQPKLMSAALVKAAKQFDVLVAALPLSDGGEEEQLKRIAELQAENDAVGQELQKQLEAAEKELKQVQELFSQVADNCLNLKKAD

Homology

[Close]

BLAST of Carg13978 vs. NCBI nr
Match: XP_022958635.1 (mediator of RNA polymerase II transcription subunit 21 [Cucurbita moschata] >XP_022958636.1 mediator of RNA polymerase II transcription subunit 21 [Cucurbita moschata] >XP_022996209.1 mediator of RNA polymerase II transcription subunit 21 [Cucurbita maxima] >XP_022996211.1 mediator of RNA polymerase II transcription subunit 21 [Cucurbita maxima] >KAG7035787.1 Mediator of RNA polymerase II transcription subunit 21 [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 258.8 bits (660), Expect = 2.6e-65
Identity = 138/138 (100.00%), Postives = 138/138 (100.00%), Query Frame = 0


Query: 1   MDIISQLQEQVNTIAALAFNTFGTLQRDAPPVRLSPNYPEPPPHEPTEDSANFAEQPKLM 60
           MDIISQLQEQVNTIAALAFNTFGTLQRDAPPVRLSPNYPEPPPHEPTEDSANFAEQPKLM
Sbjct: 1   MDIISQLQEQVNTIAALAFNTFGTLQRDAPPVRLSPNYPEPPPHEPTEDSANFAEQPKLM 60

Query: 61  SAALVKAAKQFDVLVAALPLSDGGEEEQLKRIAELQAENDAVGQELQKQLEAAEKELKQV 120
           SAALVKAAKQFDVLVAALPLSDGGEEEQLKRIAELQAENDAVGQELQKQLEAAEKELKQV
Sbjct: 61  SAALVKAAKQFDVLVAALPLSDGGEEEQLKRIAELQAENDAVGQELQKQLEAAEKELKQV 120

Query: 121 QELFSQVADNCLNLKKAD 139
           QELFSQVADNCLNLKKAD
Sbjct: 121 QELFSQVADNCLNLKKAD 138

[Close]

BLAST of Carg13978 vs. NCBI nr
Match: KAG6605829.1 (Mediator of RNA polymerase II transcription subunit 21, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 258.1 bits (658), Expect = 4.4e-65
Identity = 137/138 (99.28%), Postives = 138/138 (100.00%), Query Frame = 0


Query: 1   MDIISQLQEQVNTIAALAFNTFGTLQRDAPPVRLSPNYPEPPPHEPTEDSANFAEQPKLM 60
           MDIISQLQEQVNTIAALAFNTFGTLQRDAPPVRLSPNYPEPPPHEPTEDSANFAEQPKLM
Sbjct: 1   MDIISQLQEQVNTIAALAFNTFGTLQRDAPPVRLSPNYPEPPPHEPTEDSANFAEQPKLM 60

Query: 61  SAALVKAAKQFDVLVAALPLSDGGEEEQLKRIAELQAENDAVGQELQKQLEAAEKELKQV 120
           SAALVKAAKQFDVLVAALPLSDGGEEEQLKRIAELQAENDAVGQELQKQLEAAEKELKQV
Sbjct: 61  SAALVKAAKQFDVLVAALPLSDGGEEEQLKRIAELQAENDAVGQELQKQLEAAEKELKQV 120

Query: 121 QELFSQVADNCLNLKKAD 139
           QELFSQVADNC+NLKKAD
Sbjct: 121 QELFSQVADNCMNLKKAD 138

[Close]

BLAST of Carg13978 vs. NCBI nr
Match: XP_023532119.1 (mediator of RNA polymerase II transcription subunit 21 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 256.1 bits (653), Expect = 1.7e-64
Identity = 136/138 (98.55%), Postives = 137/138 (99.28%), Query Frame = 0


Query: 1   MDIISQLQEQVNTIAALAFNTFGTLQRDAPPVRLSPNYPEPPPHEPTEDSANFAEQPKLM 60
           MDIISQLQEQVNTIAALAFNTFGTLQRDAPPVRLSPNYPEPPPHEPT+DSANF EQPKLM
Sbjct: 1   MDIISQLQEQVNTIAALAFNTFGTLQRDAPPVRLSPNYPEPPPHEPTDDSANFVEQPKLM 60

Query: 61  SAALVKAAKQFDVLVAALPLSDGGEEEQLKRIAELQAENDAVGQELQKQLEAAEKELKQV 120
           SAALVKAAKQFDVLVAALPLSDGGEEEQLKRIAELQAENDAVGQELQKQLEAAEKELKQV
Sbjct: 61  SAALVKAAKQFDVLVAALPLSDGGEEEQLKRIAELQAENDAVGQELQKQLEAAEKELKQV 120

Query: 121 QELFSQVADNCLNLKKAD 139
           QELFSQVADNCLNLKKAD
Sbjct: 121 QELFSQVADNCLNLKKAD 138

[Close]

BLAST of Carg13978 vs. NCBI nr
Match: XP_022153730.1 (mediator of RNA polymerase II transcription subunit 21 [Momordica charantia])

HSP 1 Score: 247.3 bits (630), Expect = 7.8e-62
Identity = 134/138 (97.10%), Postives = 134/138 (97.10%), Query Frame = 0


Query: 1   MDIISQLQEQVNTIAALAFNTFGTLQRDAPPVRLSPNYPEPPPHEPTEDSANFAEQPKLM 60
           MDIISQLQEQVNTIAALAFNTFGTLQRDAPPVRLS NYPEPP  EPTEDSAN AEQPKLM
Sbjct: 1   MDIISQLQEQVNTIAALAFNTFGTLQRDAPPVRLSSNYPEPPSQEPTEDSANIAEQPKLM 60

Query: 61  SAALVKAAKQFDVLVAALPLSDGGEEEQLKRIAELQAENDAVGQELQKQLEAAEKELKQV 120
           SAALVKAAKQFDVLVAALPLSDGGEEEQLKRIAELQAENDAVGQELQKQLEAAEKELKQV
Sbjct: 61  SAALVKAAKQFDVLVAALPLSDGGEEEQLKRIAELQAENDAVGQELQKQLEAAEKELKQV 120

Query: 121 QELFSQVADNCLNLKKAD 139
           QELFSQVADNCLNLKKAD
Sbjct: 121 QELFSQVADNCLNLKKAD 138

[Close]

BLAST of Carg13978 vs. NCBI nr
Match: XP_038893979.1 (mediator of RNA polymerase II transcription subunit 21-like [Benincasa hispida] >XP_038893980.1 mediator of RNA polymerase II transcription subunit 21-like [Benincasa hispida])

HSP 1 Score: 244.6 bits (623), Expect = 5.1e-61
Identity = 131/138 (94.93%), Postives = 133/138 (96.38%), Query Frame = 0


Query: 1   MDIISQLQEQVNTIAALAFNTFGTLQRDAPPVRLSPNYPEPPPHEPTEDSANFAEQPKLM 60
           MDIISQLQEQVN IAALAFNTFGTLQRDAPPVRLSPNYPEPP HEPTEDSAN AEQPKLM
Sbjct: 1   MDIISQLQEQVNAIAALAFNTFGTLQRDAPPVRLSPNYPEPPSHEPTEDSANIAEQPKLM 60

Query: 61  SAALVKAAKQFDVLVAALPLSDGGEEEQLKRIAELQAENDAVGQELQKQLEAAEKELKQV 120
           SAALVKAAKQFD LVAALPLSDGGEEEQLKRIAELQAENDAVGQELQKQL+AAE+EL QV
Sbjct: 61  SAALVKAAKQFDALVAALPLSDGGEEEQLKRIAELQAENDAVGQELQKQLQAAERELIQV 120

Query: 121 QELFSQVADNCLNLKKAD 139
           QELFSQVADNCLNLKKAD
Sbjct: 121 QELFSQVADNCLNLKKAD 138

[Close]

BLAST of Carg13978 vs. ExPASy Swiss-Prot
Match: C0LU16 (Mediator of RNA polymerase II transcription subunit 21 OS=Arabidopsis thaliana OX=3702 GN=MED21 PE=1 SV=1)

HSP 1 Score: 203.4 bits (516), Expect = 1.7e-51
Identity = 111/139 (79.86%), Postives = 119/139 (85.61%), Query Frame = 0


Query: 1   MDIISQLQEQVNTIAALAFNTFGTLQRDAPPVRLSPNYPEPPPHEP-TEDSANFAEQPKL 60
           MDIISQLQEQVNTIAA+ FN FGTLQRDAPPV+LSPNYPEPP     T+D+  F EQPK 
Sbjct: 1   MDIISQLQEQVNTIAAITFNAFGTLQRDAPPVQLSPNYPEPPATTTVTDDATPFPEQPKQ 60

Query: 61  MSAALVKAAKQFDVLVAALPLSDGGEEEQLKRIAELQAENDAVGQELQKQLEAAEKELKQ 120
           +SA LVKAAKQFD LVAALPLS+GGE  QLKRIAELQ END VGQELQKQLEAAEKELKQ
Sbjct: 61  LSAGLVKAAKQFDALVAALPLSEGGEGAQLKRIAELQVENDLVGQELQKQLEAAEKELKQ 120

Query: 121 VQELFSQVADNCLNLKKAD 139
           VQELF Q ADNCLN+KK +
Sbjct: 121 VQELFGQAADNCLNMKKPE 139

[Close]

BLAST of Carg13978 vs. ExPASy Swiss-Prot
Match: Q7ZTI5 (Mediator of RNA polymerase II transcription subunit 21 OS=Danio rerio OX=7955 GN=med21 PE=2 SV=1)

HSP 1 Score: 48.9 bits (115), Expect = 5.3e-05
Identity = 38/137 (27.74%), Postives = 67/137 (48.91%), Query Frame = 0


Query: 2   DIISQLQEQVNTIAALAFNTFGTLQRDAPPVRLS---PNYPEPPPHEPTEDSANFAEQPK 61
           D ++QLQ+ VN++A    N  G LQ+ APP   S       +  P  PTE+ A      +
Sbjct: 3   DRLTQLQDAVNSLADQFCNAIGVLQQCAPPASFSNIQTAINKDQPSNPTEEYA------Q 62

Query: 62  LMSAALVKAAKQFDVLVAALPLSDGGEEEQLKRIAELQAENDAVGQELQKQLEAAEKELK 121
           L +A + + AK  DVL+ +LP  +     Q   + +L+ EN      L++ +   +  L+
Sbjct: 63  LFAALIARTAKDVDVLIDSLPSEESTAALQAASLRQLEEENQEAAARLEEVVYRGDALLE 122

Query: 122 QVQELFSQVADNCLNLK 136
           ++Q   + +A + L  +
Sbjct: 123 KIQTALADIAQSQLRTR 133

[Close]

BLAST of Carg13978 vs. ExPASy Swiss-Prot
Match: Q2TBU8 (Mediator of RNA polymerase II transcription subunit 21 OS=Bos taurus OX=9913 GN=MED21 PE=2 SV=1)

HSP 1 Score: 45.8 bits (107), Expect = 4.5e-04
Identity = 37/137 (27.01%), Postives = 65/137 (47.45%), Query Frame = 0


Query: 2   DIISQLQEQVNTIAALAFNTFGTLQRDAPPVRLS---PNYPEPPPHEPTEDSANFAEQPK 61
           D ++QLQ+ VN++A    N  G LQ+  PP   S       +  P  PTE+ A      +
Sbjct: 3   DRLTQLQDAVNSLADQFCNAIGVLQQCGPPASFSNIQTAINKDQPANPTEEYA------Q 62

Query: 62  LMSAALVKAAKQFDVLVAALPLSDGGEEEQLKRIAELQAENDAVGQELQKQLEAAEKELK 121
           L +A + + AK  DVL+ +LP  +     Q   + +L+ EN      L+  +   +  L+
Sbjct: 63  LFAALIARTAKDIDVLIDSLPSEESTAALQAASLYKLEEENHEAATSLEDVVYRGDMLLE 122

Query: 122 QVQELFSQVADNCLNLK 136
           ++Q   + +A + L  +
Sbjct: 123 KIQSALADIAQSQLKTR 133

[Close]

BLAST of Carg13978 vs. ExPASy TrEMBL
Match: A0A6J1K432 (Mediator of RNA polymerase II transcription subunit 21 OS=Cucurbita maxima OX=3661 GN=LOC111491504 PE=3 SV=1)

HSP 1 Score: 258.8 bits (660), Expect = 1.3e-65
Identity = 138/138 (100.00%), Postives = 138/138 (100.00%), Query Frame = 0


Query: 1   MDIISQLQEQVNTIAALAFNTFGTLQRDAPPVRLSPNYPEPPPHEPTEDSANFAEQPKLM 60
           MDIISQLQEQVNTIAALAFNTFGTLQRDAPPVRLSPNYPEPPPHEPTEDSANFAEQPKLM
Sbjct: 1   MDIISQLQEQVNTIAALAFNTFGTLQRDAPPVRLSPNYPEPPPHEPTEDSANFAEQPKLM 60

Query: 61  SAALVKAAKQFDVLVAALPLSDGGEEEQLKRIAELQAENDAVGQELQKQLEAAEKELKQV 120
           SAALVKAAKQFDVLVAALPLSDGGEEEQLKRIAELQAENDAVGQELQKQLEAAEKELKQV
Sbjct: 61  SAALVKAAKQFDVLVAALPLSDGGEEEQLKRIAELQAENDAVGQELQKQLEAAEKELKQV 120

Query: 121 QELFSQVADNCLNLKKAD 139
           QELFSQVADNCLNLKKAD
Sbjct: 121 QELFSQVADNCLNLKKAD 138

[Close]

BLAST of Carg13978 vs. ExPASy TrEMBL
Match: A0A6J1H2M0 (Mediator of RNA polymerase II transcription subunit 21 OS=Cucurbita moschata OX=3662 GN=LOC111459801 PE=3 SV=1)

HSP 1 Score: 258.8 bits (660), Expect = 1.3e-65
Identity = 138/138 (100.00%), Postives = 138/138 (100.00%), Query Frame = 0


Query: 1   MDIISQLQEQVNTIAALAFNTFGTLQRDAPPVRLSPNYPEPPPHEPTEDSANFAEQPKLM 60
           MDIISQLQEQVNTIAALAFNTFGTLQRDAPPVRLSPNYPEPPPHEPTEDSANFAEQPKLM
Sbjct: 1   MDIISQLQEQVNTIAALAFNTFGTLQRDAPPVRLSPNYPEPPPHEPTEDSANFAEQPKLM 60

Query: 61  SAALVKAAKQFDVLVAALPLSDGGEEEQLKRIAELQAENDAVGQELQKQLEAAEKELKQV 120
           SAALVKAAKQFDVLVAALPLSDGGEEEQLKRIAELQAENDAVGQELQKQLEAAEKELKQV
Sbjct: 61  SAALVKAAKQFDVLVAALPLSDGGEEEQLKRIAELQAENDAVGQELQKQLEAAEKELKQV 120

Query: 121 QELFSQVADNCLNLKKAD 139
           QELFSQVADNCLNLKKAD
Sbjct: 121 QELFSQVADNCLNLKKAD 138

[Close]

BLAST of Carg13978 vs. ExPASy TrEMBL
Match: A0A6J1DHM9 (Mediator of RNA polymerase II transcription subunit 21 OS=Momordica charantia OX=3673 GN=LOC111021179 PE=3 SV=1)

HSP 1 Score: 247.3 bits (630), Expect = 3.8e-62
Identity = 134/138 (97.10%), Postives = 134/138 (97.10%), Query Frame = 0


Query: 1   MDIISQLQEQVNTIAALAFNTFGTLQRDAPPVRLSPNYPEPPPHEPTEDSANFAEQPKLM 60
           MDIISQLQEQVNTIAALAFNTFGTLQRDAPPVRLS NYPEPP  EPTEDSAN AEQPKLM
Sbjct: 1   MDIISQLQEQVNTIAALAFNTFGTLQRDAPPVRLSSNYPEPPSQEPTEDSANIAEQPKLM 60

Query: 61  SAALVKAAKQFDVLVAALPLSDGGEEEQLKRIAELQAENDAVGQELQKQLEAAEKELKQV 120
           SAALVKAAKQFDVLVAALPLSDGGEEEQLKRIAELQAENDAVGQELQKQLEAAEKELKQV
Sbjct: 61  SAALVKAAKQFDVLVAALPLSDGGEEEQLKRIAELQAENDAVGQELQKQLEAAEKELKQV 120

Query: 121 QELFSQVADNCLNLKKAD 139
           QELFSQVADNCLNLKKAD
Sbjct: 121 QELFSQVADNCLNLKKAD 138

[Close]

BLAST of Carg13978 vs. ExPASy TrEMBL
Match: A0A0A0KZ00 (Mediator of RNA polymerase II transcription subunit 21 OS=Cucumis sativus OX=3659 GN=Csa_4G268030 PE=3 SV=1)

HSP 1 Score: 240.0 bits (611), Expect = 6.0e-60
Identity = 129/138 (93.48%), Postives = 131/138 (94.93%), Query Frame = 0


Query: 1   MDIISQLQEQVNTIAALAFNTFGTLQRDAPPVRLSPNYPEPPPHEPTEDSANFAEQPKLM 60
           MDIISQLQEQVNTIAALAFNTFGTLQRDAPPVRLSPNYPEPP  EPTED+A  AEQPKLM
Sbjct: 1   MDIISQLQEQVNTIAALAFNTFGTLQRDAPPVRLSPNYPEPPSQEPTEDNATIAEQPKLM 60

Query: 61  SAALVKAAKQFDVLVAALPLSDGGEEEQLKRIAELQAENDAVGQELQKQLEAAEKELKQV 120
           SAALVKAAKQFD LVAALPLSDGGEEEQLKRIAELQAEND VGQELQKQL+AAEKEL QV
Sbjct: 61  SAALVKAAKQFDALVAALPLSDGGEEEQLKRIAELQAENDVVGQELQKQLQAAEKELVQV 120

Query: 121 QELFSQVADNCLNLKKAD 139
           QELFSQVADNCLNLKKAD
Sbjct: 121 QELFSQVADNCLNLKKAD 138

[Close]

BLAST of Carg13978 vs. ExPASy TrEMBL
Match: A0A5A7TEZ9 (Mediator of RNA polymerase II transcription subunit 21 OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold366G00920 PE=3 SV=1)

HSP 1 Score: 239.6 bits (610), Expect = 7.9e-60
Identity = 129/138 (93.48%), Postives = 131/138 (94.93%), Query Frame = 0


Query: 1   MDIISQLQEQVNTIAALAFNTFGTLQRDAPPVRLSPNYPEPPPHEPTEDSANFAEQPKLM 60
           MDIISQLQEQVNTIAALAFNTFGTLQRDAPPVRLSPNYPEPP  EPTEDSA  AEQPKLM
Sbjct: 1   MDIISQLQEQVNTIAALAFNTFGTLQRDAPPVRLSPNYPEPPSQEPTEDSATIAEQPKLM 60

Query: 61  SAALVKAAKQFDVLVAALPLSDGGEEEQLKRIAELQAENDAVGQELQKQLEAAEKELKQV 120
           SAALVKAAKQFD LVAALPLSDGGEEEQLKRIAELQAEND VGQELQKQL+AAE+EL QV
Sbjct: 61  SAALVKAAKQFDALVAALPLSDGGEEEQLKRIAELQAENDVVGQELQKQLQAAEQELVQV 120

Query: 121 QELFSQVADNCLNLKKAD 139
           QELFSQVADNCLNLKKAD
Sbjct: 121 QELFSQVADNCLNLKKAD 138

[Close]

BLAST of Carg13978 vs. TAIR 10
Match: AT4G04780.1 (mediator 21 )

HSP 1 Score: 203.4 bits (516), Expect = 1.2e-52
Identity = 111/139 (79.86%), Postives = 119/139 (85.61%), Query Frame = 0


Query: 1   MDIISQLQEQVNTIAALAFNTFGTLQRDAPPVRLSPNYPEPPPHEP-TEDSANFAEQPKL 60
           MDIISQLQEQVNTIAA+ FN FGTLQRDAPPV+LSPNYPEPP     T+D+  F EQPK 
Sbjct: 1   MDIISQLQEQVNTIAAITFNAFGTLQRDAPPVQLSPNYPEPPATTTVTDDATPFPEQPKQ 60

Query: 61  MSAALVKAAKQFDVLVAALPLSDGGEEEQLKRIAELQAENDAVGQELQKQLEAAEKELKQ 120
           +SA LVKAAKQFD LVAALPLS+GGE  QLKRIAELQ END VGQELQKQLEAAEKELKQ
Sbjct: 61  LSAGLVKAAKQFDALVAALPLSEGGEGAQLKRIAELQVENDLVGQELQKQLEAAEKELKQ 120

Query: 121 VQELFSQVADNCLNLKKAD 139
           VQELF Q ADNCLN+KK +
Sbjct: 121 VQELFGQAADNCLNMKKPE 139

The following BLAST results are available for this feature:

BLAST of Carg13978 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_022958635.1	2.6e-65	100.00	mediator of RNA polymerase II transcription subunit 21 [Cucurbita moschata] >XP_...	[more]
KAG6605829.1	4.4e-65	99.28	Mediator of RNA polymerase II transcription subunit 21, partial [Cucurbita argyr...	[more]
XP_023532119.1	1.7e-64	98.55	mediator of RNA polymerase II transcription subunit 21 [Cucurbita pepo subsp. pe...	[more]
XP_022153730.1	7.8e-62	97.10	mediator of RNA polymerase II transcription subunit 21 [Momordica charantia]	[more]
XP_038893979.1	5.1e-61	94.93	mediator of RNA polymerase II transcription subunit 21-like [Benincasa hispida] ...	[more]

BLAST of Carg13978 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
C0LU16	1.7e-51	79.86	Mediator of RNA polymerase II transcription subunit 21 OS=Arabidopsis thaliana O...	[more]
Q7ZTI5	5.3e-05	27.74	Mediator of RNA polymerase II transcription subunit 21 OS=Danio rerio OX=7955 GN...	[more]
Q2TBU8	4.5e-04	27.01	Mediator of RNA polymerase II transcription subunit 21 OS=Bos taurus OX=9913 GN=...	[more]

BLAST of Carg13978 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1K432	1.3e-65	100.00	Mediator of RNA polymerase II transcription subunit 21 OS=Cucurbita maxima OX=36...	[more]
A0A6J1H2M0	1.3e-65	100.00	Mediator of RNA polymerase II transcription subunit 21 OS=Cucurbita moschata OX=...	[more]
A0A6J1DHM9	3.8e-62	97.10	Mediator of RNA polymerase II transcription subunit 21 OS=Momordica charantia OX...	[more]
A0A0A0KZ00	6.0e-60	93.48	Mediator of RNA polymerase II transcription subunit 21 OS=Cucumis sativus OX=365...	[more]
A0A5A7TEZ9	7.9e-60	93.48	Mediator of RNA polymerase II transcription subunit 21 OS=Cucumis melo var. maku...	[more]

BLAST of Carg13978 vs. TAIR 10


Match Name	E-value	Identity	Description
AT4G04780.1	1.2e-52	79.86	mediator 21	[more]

InterPro

Analysis Name: InterPro Annotations of Silver-seed gourd (SMH-JMG-627) v2
Date Performed: 2021-10-25

Position :

Zoom :

x 1


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
None	No IPR available	COILS	Coil	Coil	coord: 85..130
None	No IPR available	GENE3D	6.10.280.10	Mediator complex, subunit Med21	coord: 1..130 e-value: 1.3E-30 score: 108.2
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 28..51
IPR021384	Mediator complex, subunit Med21	PFAM	PF11221	Med21	coord: 1..128 e-value: 2.7E-29 score: 102.3
IPR021384	Mediator complex, subunit Med21	PANTHER	PTHR13381	RNA POLYMERASE II HOLOENZYME COMPONENT SRB7	coord: 1..136
IPR037212	Mediator complex, subunit Med7/Med21-like	SUPERFAMILY	140718	Mediator hinge subcomplex-like	coord: 1..126

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Carg13978-RA	Carg13978-RA	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0050832	defense response to fungus
biological_process	GO:0006357	regulation of transcription by RNA polymerase II
cellular_component	GO:0016021	integral component of membrane
cellular_component	GO:0016592	mediator complex
cellular_component	GO:0043078	polar nucleus
molecular_function	GO:0003712	transcription coregulator activity