Carg13881 (gene) Silver-seed gourd (SMH-JMG-627) v2

Overview
NameCarg13881
Typegene
OrganismCucurbita argyrosperma subsp. argyrosperma cv. SMH-JMG-627 (Silver-seed gourd (SMH-JMG-627) v2)
Description7-dehydrocholesterol reductase
LocationCarg_Chr08: 4728145 .. 4733495 (-)
RNA-Seq ExpressionCarg13881
SyntenyCarg13881
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRpolypeptideCDSthree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
AAAATAATAATAATAGTAATATTACATCGGAAGTCCGAAGTCCCAGCGAGTGTCAATTCCCGGCGGAATTTGCTCTGACTACGGTCTTCCTGAAACTCCGGCGCCTCAAAAATGGCGGAAGGATCCAACACCGTCCACTCGCCCCTCGTCACTTATGCTTCCATGTTATCTCTTCTCACTCTCTGCCCTCCCTTTGTCATCTTCCTGTAATTCCCTCTGCTTCAAATGGATCTCATTCACTTTCTCTTTTTGTCAAAGATCGCCTTCATCATTCAATGTTCCTCTAATTTCAACGATCCATTGCGAATCTTGCTTCGCTATGCGCACATTTCGTGTTTGTTTTTGTTTGAATATCTCAACGTTTCTTCTCTGATGCAGATGGTACACAATGGTCCACGCCGATGGGTCTGTTTCCCAGACTATGGCTTACTTATACCACAATGGATTGAGGGGGTTTGTAGATATATGGCCACCGCCCTCTGCTACTGCTTTCAAATTAATTGGATCTTACGCTGCCTTTGAGGCCGCCCTTCAGCTTCTTTTACCTGGAAAAACGGTTTATGGCCCGATCTCCCCTACGGGAAATCAACCTGTTTACAAGGTATTTGGATATTAGATTCTCGATCACTCTGTTTCAGTAGAAGAAACTACTAATGCCTCGATGTTCTGTTCATAATTTATTGTTCCTGTTTCTGTGTTGCAGGCAAATGGTGTGGCTGCGTACGTAGTGACTCTGGTTACTTATATTGCTCTTTGGTGGTATGATTTCGTTCATCTTTAGTAATTGAAATATTTGAGGTTGATCTATTCTAGATGTCCGTGATGAATTTCAAACTTCGTGATCCCTTTGGGGGAAAAAGTATATACATCAGTATGTGATATGTGATTCTGTAACAGCCCAAACCCCCGCTAGTCGATATTGTCCTCTTTGGGCTTTCTCTTTGAGGGAGAGGTTTTAAGACACATTCACTAGGGAGAGGTTTCCACACCATGTTTCGTTCCTCTCTCCAACCAATGTGAGATCTCACAATCCTCCTCTCTTAGAGACTAGTGTGTTCATTGGCACACCACCCGATGTCTAGCTCTGATACCATTGTAACGGCCCAGGCCCACTGCTAGCAGATATTGTCCTCTTTGGGCTAAAAATTTGGACCATTTCTCGATTTGTGTGTCATCCATGTGCAGGGGCAATACTAATCTTCTCTGTATCGTTCAAATTTTATCGTGAAAACCTCTCCCCTAGTAGACGCGTTTTAAAAACCTTGAGGGGAATCTTGAAAGGGAAGTCCAAAGCGGACAATATCTGCTAGCGGTGGCCCAGACCCTTACAAATTCTGTACTAATATTTCGTTGTTCTGTTATATTCAATCTATATCTTTTGTGCTAGAATTATATGCTTATACATGATTTGGACAGGAAATATCTTTTCTGCTCTCTGGATTTCTGATGGTTTTTCTTTTCCAGTTTCATAATTTAATTGAATGTTTTAATCTTCAGGTTTGGGATATTCAATCCAACAGTTGTTTATGATCATTTGGGAGAAATATTTTCAGCTCTTATTATTGGAAGCTTCATCTTTTGCATTGTTCTATACATAAAAGTGAGTATTTAGGCTGTTGTGTTTGATACCTGTTGAAGTTAAGTATATATATATATGCTATTTGTATGTATAACAGTGGTGTTTGCATGTATAAGGGCTGTGTGGCGCCATCTTCCTCTGATTCTGGTTCGTCTGGTAACATAATAATTGATTTCTACTGGGTAAGTTTTCACATCCCTTTGATATTTATAATTTGATGTGATAATTTTCACTTTTCTTAAGTTAAGAATAAGAAAGAAACTCAGTTGTTTTAAAGATGAATGAGGGTACAGAGAAGGTGACACAAAAAAGAGGCCCCATCCAACATGGGAACAAGACAACAAAAAGAAAAGATAAAATTACCAGATTATAAAAAGGGACCTAAAATAATGAACAGCCAAACAACCCTTATCTCAAAATGGAGGACTTGAAACACTTCCTCCATCACTGTGTGTAAACTAGAACTTGGAACCAGGAAAATACCAAAAGTCTACCAAAAACAACACACCTTTTGGAACAATGTGATGCATATTATAGATGATTATTGAACTAAGTTAACTGTGAACGAACTAATGAACTCTCGGGATACAATTATTGGGGTAATTTGGCACAAATCAGATGATAGTCCTCTAAAAAACTCAAGTAGTTTGTATATGCACTTGTTGTCTTGAAAAGATCCATGGTTTTATTATGTTATAATGCTTATCTGCTTTTTACTTCAGCAATCAGTCTGTCTTTAAGTGCTAGTCTGCTTAATATGTATGGTATGATAACAAAAAATCTCCACTAGAATTTCATGTTAAATGCTCTTAGCCTTGTGAAGTTATATCTTTATATGCAGCTTATACGCTTTCAATTTGAAAATCGGGAAAAAGTAGTATACTCTATACTCTAGGGTTCATCTTCATATAACTTGTTTATGTTTTTCTCCCGTGTTTTTTTTTTTTAAATTTTTATTTGCTTAATTTTCTCCTTAGTTCCATTTTAAAGCATCAGTAAATTGAATAAGTACTAGAGTATATGTGAGATCCCTACGTCGATGGGAGAGAAGAACGAGTGCTAGTGAGGACGCTGGGTCTCGAAGGGGGTGGATTGTGAGATCCCACATCGATTGGAGAGAGAGAAGGAGGAACGAGTGTCACCGAAAGGGGGTGGATTGTGAGATCTCACATCGATTGGAGAGGGGAACGAAGTATTGTTTATAAGGGTATGGAAACCTCTCCCTAGCAAACGCGTTTTAAAAACCTTGAGGAAAAACCCAAAAGGGAAAACCCAAAGAGGACAATATCTGCTAGTGGTGGTCTTTGACTAGTATTTTGGTTGATCGCATGATTAACTTGGCTAATCTAGGTCTTGTTGATATTCTAATAACACCTTTGTTATTTACTTTCTTGCAGGGCATGGAGTTGTACCCGCGTATTGGCAAACATTTTGACATTAAGGTTTTCACAAACTGCAGATTTGGGATGATGTCTTGGGCAGTTCTTGCTGTGACCTATTGCATAAAGCAGGTAGGTGTTCTAAAAGATCACCAAGTATGAAACATTATCCTACATTTGTGGAACACTATCCTTCATGTGAGGTTTACCAAATAGTATTCAGAATAATGGGATTCAAGCCTCATTTAATTAGTTGGTACCTTTGCTTTATCTTTTATCAGTATTTTCTCACATTACATGATATAACATTCACAGATGTTCTCTAGGCTTGTAGGTTCTATGATATTCAAGGTGTTGAGATGTAATTGTTGCATAATTATCTTCTCATTTCGTCCATTTCAGTACGAAAAGAACGGGACAATAGCTGATTCGATGCTTGTAAATACTGCATTGATGTTGGCTTATGTTACAAAGTTTTTTTGGTGGGAAGCTGGGTACTGGAACACAATGGACATAGCGCATGATAGAGGTTAGCTTATATTGTATGTTTTTCTTTGTTTAACATGTCATTTCTTTTCATACTCTTCTGTTATTAATAAAATGCTCGAGGTGAGCTGACTCGTTTTCGACCTTGAAAAATGTGATGCACAAATCTTCAACTCTTATGCAGCTGAACTATACTAGGATGTGATTTTAATGGTCCATTTATCTTGTCAGAATTAAGAATAAATAAAACATGTTATCCTCTTTGTATTATCAATCTAATTACTCCTGGAAATTGGTAACTCGGATTTTGCAGCTGGTTTTTATATTTGTTGGGGCTGCTTAGTGTGGGTTCCGTCTGTATATACCTCTCCTGGCATGTATTTGGTCAACCATCCTGTACACCTTGGAACTCAGGTATCCCTTCTATCTAGTTAACTTCTAACATTAAGCAATTAATTTGTACTGAAACATGCTGGATCATCACATATTCTGGAGATTCGTGGAGACTCGTTAGCTTTTAGATGGTTGCTGGTTAGCCAATGTGGAAGATATCGACTCTTAAACCGATCCTTTCTACTCGTATAATTCTTGATGGATTGTGCTACTTTCCTAATAGTCCCTCCTTTTGGTTTTCAGTTGGCAGTATATATTCTAGTAGCAGGCATGCTTTGCATATATATCAACTACGATTGCGACAGGCAGAGACAAGAATTTCGGCGAAAAAACGGGAAGTGCTTGGTTTGGGGAAAAGCTCCATCTAAGGTTTTGACGAGAATTCTGTCTAACAGTTCTTATGGAACGATCTAGTGATACGATGTGACGAAATCTGGTATTAATTTGACAGATAGTAGCTTCATACACCACTACATCTGGGGAAACCAAAACTAGCCTTCTTTTAACCTCTGGATGGTGAGTTTTCATTTGCTTCATTTTCTCACATATACATAAGTGTGTGAGTGGGATTTTGTTGTTGGTGCAGGTGGGGCTTAGCAAGGCATTTTCATTATGTACCAGAAATTTTGGCTGCATTTTTCTGGACAGTTCCAGCCCTTTTCAGTCATGTAAGCATCACCTATTCTAATCAATACAAACAGAAAGAAGAATTATATTGTTTATTTGGTAATTGTTGGTTTGGATGTCCTCCTTTGCAGTTTCTACCATATTTCTATGTGATATTCCTTACTATCCTTCTGGTCGACCGAGCTAAAAGGGATGACGATCGATGTCGGTCCAAGTAAGTTAATCAGTTCGGTTCCAGCTGATATGGGATTGAGAGTACCTCCTACTTTGGTTGATGATTTTGGTTTCATGCAGGTATGGCAAATATTGGAAACTGTACTGCGAGAAAGTGCCTTACAGAATCGTTCCTGGAATATGCTAATACGGCTTCCTACATTTGTATATCACTTATAGTACGATCTAAATACTTTGTTTGTTTGTTATTACCTTTGTTGCTCGCTTCACTTCATGTAAAACCTTATAATATTGTAACACTTTTTTCATCCTTCTTTATCTGTTGTTGTTTTTGTATGCAAGTTACTAGAGCTTTCACCATTTTCTCCCATTGCTCTCTACCTCTCTTGTCGACAATCTCCCGCTGCAGCGACCGTCTTCTTGTCAACACAATTCGTCGTGGTCACACCTTCCCTCGCCTTCATCGTTGCCAATGTCGTCTCTCACTCTTGCCTCCCTCCCCATTTGCCTAACACTATCCCTCGCCTCCATGGCAGCCTATACCGTCTCTTGCTCTAGCCTCTAGCCTCGCGCCTCACTGTGGCTGTCACGTCTGTCTCCGATGACCCAATGTTGTAGGGTTCAGAATGGTAAAACTATAGATAAAATAAATTTTCTAGGAAAAGAAAGTTTGGTGCGGAAACTGACTAGTCAGTTGGCATGTTTAATCCATA

mRNA sequence

AAAATAATAATAATAGTAATATTACATCGGAAGTCCGAAGTCCCAGCGAGTGTCAATTCCCGGCGGAATTTGCTCTGACTACGGTCTTCCTGAAACTCCGGCGCCTCAAAAATGGCGGAAGGATCCAACACCGTCCACTCGCCCCTCGTCACTTATGCTTCCATGTTATCTCTTCTCACTCTCTGCCCTCCCTTTGTCATCTTCCTATGGTACACAATGGTCCACGCCGATGGGTCTGTTTCCCAGACTATGGCTTACTTATACCACAATGGATTGAGGGGGTTTGTAGATATATGGCCACCGCCCTCTGCTACTGCTTTCAAATTAATTGGATCTTACGCTGCCTTTGAGGCCGCCCTTCAGCTTCTTTTACCTGGAAAAACGGTTTATGGCCCGATCTCCCCTACGGGAAATCAACCTGTTTACAAGGCAAATGGTGTGGCTGCGTACGTAGTGACTCTGGTTACTTATATTGCTCTTTGGTGGTTTGGGATATTCAATCCAACAGTTGTTTATGATCATTTGGGAGAAATATTTTCAGCTCTTATTATTGGAAGCTTCATCTTTTGCATTGTTCTATACATAAAAGGCTGTGTGGCGCCATCTTCCTCTGATTCTGGTTCGTCTGGTAACATAATAATTGATTTCTACTGGGGCATGGAGTTGTACCCGCGTATTGGCAAACATTTTGACATTAAGGTTTTCACAAACTGCAGATTTGGGATGATGTCTTGGGCAGTTCTTGCTGTGACCTATTGCATAAAGCAGTACGAAAAGAACGGGACAATAGCTGATTCGATGCTTGTAAATACTGCATTGATGTTGGCTTATGTTACAAAGTTTTTTTGGTGGGAAGCTGGGTACTGGAACACAATGGACATAGCGCATGATAGAGCTGGTTTTTATATTTGTTGGGGCTGCTTAGTGTGGGTTCCGTCTGTATATACCTCTCCTGGCATGTATTTGGTCAACCATCCTGTACACCTTGGAACTCAGTTGGCAGTATATATTCTAGTAGCAGGCATGCTTTGCATATATATCAACTACGATTGCGACAGGCAGAGACAAGAATTTCGGCGAAAAAACGGGAAGTGCTTGGTTTGGGGAAAAGCTCCATCTAAGATAGTAGCTTCATACACCACTACATCTGGGGAAACCAAAACTAGCCTTCTTTTAACCTCTGGATGGTGGGGCTTAGCAAGGCATTTTCATTATGTACCAGAAATTTTGGCTGCATTTTTCTGGACAGTTCCAGCCCTTTTCAGTCATTTTCTACCATATTTCTATGTGATATTCCTTACTATCCTTCTGGTCGACCGAGCTAAAAGGGATGACGATCGATGTCGGTCCAAGTATGGCAAATATTGGAAACTGTACTGCGAGAAAGTGCCTTACAGAATCGTTCCTGGAATATGCTAATACGGCTTCCTACATTTGTATATCACTTATAGTACGATCTAAATACTTTGTTTGTTTGTTATTACCTTTGTTGCTCGCTTCACTTCATGTAAAACCTTATAATATTGTAACACTTTTTTCATCCTTCTTTATCTGTTGTTGTTTTTGTATGCAAGTTACTAGAGCTTTCACCATTTTCTCCCATTGCTCTCTACCTCTCTTGTCGACAATCTCCCGCTGCAGCGACCGTCTTCTTGTCAACACAATTCGTCGTGGTCACACCTTCCCTCGCCTTCATCGTTGCCAATGTCGTCTCTCACTCTTGCCTCCCTCCCCATTTGCCTAACACTATCCCTCGCCTCCATGGCAGCCTATACCGTCTCTTGCTCTAGCCTCTAGCCTCGCGCCTCACTGTGGCTGTCACGTCTGTCTCCGATGACCCAATGTTGTAGGGTTCAGAATGGTAAAACTATAGATAAAATAAATTTTCTAGGAAAAGAAAGTTTGGTGCGGAAACTGACTAGTCAGTTGGCATGTTTAATCCATA

Coding sequence (CDS)

ATGGCGGAAGGATCCAACACCGTCCACTCGCCCCTCGTCACTTATGCTTCCATGTTATCTCTTCTCACTCTCTGCCCTCCCTTTGTCATCTTCCTATGGTACACAATGGTCCACGCCGATGGGTCTGTTTCCCAGACTATGGCTTACTTATACCACAATGGATTGAGGGGGTTTGTAGATATATGGCCACCGCCCTCTGCTACTGCTTTCAAATTAATTGGATCTTACGCTGCCTTTGAGGCCGCCCTTCAGCTTCTTTTACCTGGAAAAACGGTTTATGGCCCGATCTCCCCTACGGGAAATCAACCTGTTTACAAGGCAAATGGTGTGGCTGCGTACGTAGTGACTCTGGTTACTTATATTGCTCTTTGGTGGTTTGGGATATTCAATCCAACAGTTGTTTATGATCATTTGGGAGAAATATTTTCAGCTCTTATTATTGGAAGCTTCATCTTTTGCATTGTTCTATACATAAAAGGCTGTGTGGCGCCATCTTCCTCTGATTCTGGTTCGTCTGGTAACATAATAATTGATTTCTACTGGGGCATGGAGTTGTACCCGCGTATTGGCAAACATTTTGACATTAAGGTTTTCACAAACTGCAGATTTGGGATGATGTCTTGGGCAGTTCTTGCTGTGACCTATTGCATAAAGCAGTACGAAAAGAACGGGACAATAGCTGATTCGATGCTTGTAAATACTGCATTGATGTTGGCTTATGTTACAAAGTTTTTTTGGTGGGAAGCTGGGTACTGGAACACAATGGACATAGCGCATGATAGAGCTGGTTTTTATATTTGTTGGGGCTGCTTAGTGTGGGTTCCGTCTGTATATACCTCTCCTGGCATGTATTTGGTCAACCATCCTGTACACCTTGGAACTCAGTTGGCAGTATATATTCTAGTAGCAGGCATGCTTTGCATATATATCAACTACGATTGCGACAGGCAGAGACAAGAATTTCGGCGAAAAAACGGGAAGTGCTTGGTTTGGGGAAAAGCTCCATCTAAGATAGTAGCTTCATACACCACTACATCTGGGGAAACCAAAACTAGCCTTCTTTTAACCTCTGGATGGTGGGGCTTAGCAAGGCATTTTCATTATGTACCAGAAATTTTGGCTGCATTTTTCTGGACAGTTCCAGCCCTTTTCAGTCATTTTCTACCATATTTCTATGTGATATTCCTTACTATCCTTCTGGTCGACCGAGCTAAAAGGGATGACGATCGATGTCGGTCCAAGTATGGCAAATATTGGAAACTGTACTGCGAGAAAGTGCCTTACAGAATCGTTCCTGGAATATGCTAA

Protein sequence

MAEGSNTVHSPLVTYASMLSLLTLCPPFVIFLWYTMVHADGSVSQTMAYLYHNGLRGFVDIWPPPSATAFKLIGSYAAFEAALQLLLPGKTVYGPISPTGNQPVYKANGVAAYVVTLVTYIALWWFGIFNPTVVYDHLGEIFSALIIGSFIFCIVLYIKGCVAPSSSDSGSSGNIIIDFYWGMELYPRIGKHFDIKVFTNCRFGMMSWAVLAVTYCIKQYEKNGTIADSMLVNTALMLAYVTKFFWWEAGYWNTMDIAHDRAGFYICWGCLVWVPSVYTSPGMYLVNHPVHLGTQLAVYILVAGMLCIYINYDCDRQRQEFRRKNGKCLVWGKAPSKIVASYTTTSGETKTSLLLTSGWWGLARHFHYVPEILAAFFWTVPALFSHFLPYFYVIFLTILLVDRAKRDDDRCRSKYGKYWKLYCEKVPYRIVPGIC
Homology
BLAST of Carg13881 vs. NCBI nr
Match: KAG7025947.1 (7-dehydrocholesterol reductase [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 919.8 bits (2376), Expect = 8.5e-264
Identity = 435/435 (100.00%), Postives = 435/435 (100.00%), Query Frame = 0

Query: 1   MAEGSNTVHSPLVTYASMLSLLTLCPPFVIFLWYTMVHADGSVSQTMAYLYHNGLRGFVD 60
           MAEGSNTVHSPLVTYASMLSLLTLCPPFVIFLWYTMVHADGSVSQTMAYLYHNGLRGFVD
Sbjct: 1   MAEGSNTVHSPLVTYASMLSLLTLCPPFVIFLWYTMVHADGSVSQTMAYLYHNGLRGFVD 60

Query: 61  IWPPPSATAFKLIGSYAAFEAALQLLLPGKTVYGPISPTGNQPVYKANGVAAYVVTLVTY 120
           IWPPPSATAFKLIGSYAAFEAALQLLLPGKTVYGPISPTGNQPVYKANGVAAYVVTLVTY
Sbjct: 61  IWPPPSATAFKLIGSYAAFEAALQLLLPGKTVYGPISPTGNQPVYKANGVAAYVVTLVTY 120

Query: 121 IALWWFGIFNPTVVYDHLGEIFSALIIGSFIFCIVLYIKGCVAPSSSDSGSSGNIIIDFY 180
           IALWWFGIFNPTVVYDHLGEIFSALIIGSFIFCIVLYIKGCVAPSSSDSGSSGNIIIDFY
Sbjct: 121 IALWWFGIFNPTVVYDHLGEIFSALIIGSFIFCIVLYIKGCVAPSSSDSGSSGNIIIDFY 180

Query: 181 WGMELYPRIGKHFDIKVFTNCRFGMMSWAVLAVTYCIKQYEKNGTIADSMLVNTALMLAY 240
           WGMELYPRIGKHFDIKVFTNCRFGMMSWAVLAVTYCIKQYEKNGTIADSMLVNTALMLAY
Sbjct: 181 WGMELYPRIGKHFDIKVFTNCRFGMMSWAVLAVTYCIKQYEKNGTIADSMLVNTALMLAY 240

Query: 241 VTKFFWWEAGYWNTMDIAHDRAGFYICWGCLVWVPSVYTSPGMYLVNHPVHLGTQLAVYI 300
           VTKFFWWEAGYWNTMDIAHDRAGFYICWGCLVWVPSVYTSPGMYLVNHPVHLGTQLAVYI
Sbjct: 241 VTKFFWWEAGYWNTMDIAHDRAGFYICWGCLVWVPSVYTSPGMYLVNHPVHLGTQLAVYI 300

Query: 301 LVAGMLCIYINYDCDRQRQEFRRKNGKCLVWGKAPSKIVASYTTTSGETKTSLLLTSGWW 360
           LVAGMLCIYINYDCDRQRQEFRRKNGKCLVWGKAPSKIVASYTTTSGETKTSLLLTSGWW
Sbjct: 301 LVAGMLCIYINYDCDRQRQEFRRKNGKCLVWGKAPSKIVASYTTTSGETKTSLLLTSGWW 360

Query: 361 GLARHFHYVPEILAAFFWTVPALFSHFLPYFYVIFLTILLVDRAKRDDDRCRSKYGKYWK 420
           GLARHFHYVPEILAAFFWTVPALFSHFLPYFYVIFLTILLVDRAKRDDDRCRSKYGKYWK
Sbjct: 361 GLARHFHYVPEILAAFFWTVPALFSHFLPYFYVIFLTILLVDRAKRDDDRCRSKYGKYWK 420

Query: 421 LYCEKVPYRIVPGIC 436
           LYCEKVPYRIVPGIC
Sbjct: 421 LYCEKVPYRIVPGIC 435

BLAST of Carg13881 vs. NCBI nr
Match: KAG6593601.1 (7-dehydrocholesterol reductase, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 918.7 bits (2373), Expect = 1.9e-263
Identity = 434/435 (99.77%), Postives = 435/435 (100.00%), Query Frame = 0

Query: 1   MAEGSNTVHSPLVTYASMLSLLTLCPPFVIFLWYTMVHADGSVSQTMAYLYHNGLRGFVD 60
           MAEGSNTVHSPLVTYASMLSLLTLCPPFVIFLWYTMVHADGSVSQTMAYLYHNGLRGFVD
Sbjct: 1   MAEGSNTVHSPLVTYASMLSLLTLCPPFVIFLWYTMVHADGSVSQTMAYLYHNGLRGFVD 60

Query: 61  IWPPPSATAFKLIGSYAAFEAALQLLLPGKTVYGPISPTGNQPVYKANGVAAYVVTLVTY 120
           IWPPPSATAFKLIGSYAAFEAALQLLLPGKTVYGPISPTGNQPVYKANGVAAYVVTLVTY
Sbjct: 61  IWPPPSATAFKLIGSYAAFEAALQLLLPGKTVYGPISPTGNQPVYKANGVAAYVVTLVTY 120

Query: 121 IALWWFGIFNPTVVYDHLGEIFSALIIGSFIFCIVLYIKGCVAPSSSDSGSSGNIIIDFY 180
           IALWWFGIFNPTVVYDHLGEIFSALIIGSFIFCIVLYIKGCVAPSSSDSGSSGNIIIDFY
Sbjct: 121 IALWWFGIFNPTVVYDHLGEIFSALIIGSFIFCIVLYIKGCVAPSSSDSGSSGNIIIDFY 180

Query: 181 WGMELYPRIGKHFDIKVFTNCRFGMMSWAVLAVTYCIKQYEKNGTIADSMLVNTALMLAY 240
           WGMELYPRIGKHFDIKVFTNCRFGMMSWAVLAVTYCIKQYEKNGTIADSMLVNTALMLAY
Sbjct: 181 WGMELYPRIGKHFDIKVFTNCRFGMMSWAVLAVTYCIKQYEKNGTIADSMLVNTALMLAY 240

Query: 241 VTKFFWWEAGYWNTMDIAHDRAGFYICWGCLVWVPSVYTSPGMYLVNHPVHLGTQLAVYI 300
           VTKFFWWEAGYWNTMDIAHDRAGFYICWGCLVWVPSVYTSPGMYLVNHPVHLGTQLAVYI
Sbjct: 241 VTKFFWWEAGYWNTMDIAHDRAGFYICWGCLVWVPSVYTSPGMYLVNHPVHLGTQLAVYI 300

Query: 301 LVAGMLCIYINYDCDRQRQEFRRKNGKCLVWGKAPSKIVASYTTTSGETKTSLLLTSGWW 360
           LVAGMLCIYINYDCDRQRQEFRRKNGKCLVWGKAPSKIVASYTTTSGETKTSLLLTSGWW
Sbjct: 301 LVAGMLCIYINYDCDRQRQEFRRKNGKCLVWGKAPSKIVASYTTTSGETKTSLLLTSGWW 360

Query: 361 GLARHFHYVPEILAAFFWTVPALFSHFLPYFYVIFLTILLVDRAKRDDDRCRSKYGKYWK 420
           GLARHFHYVPEILAAFFWTVPALF+HFLPYFYVIFLTILLVDRAKRDDDRCRSKYGKYWK
Sbjct: 361 GLARHFHYVPEILAAFFWTVPALFNHFLPYFYVIFLTILLVDRAKRDDDRCRSKYGKYWK 420

Query: 421 LYCEKVPYRIVPGIC 436
           LYCEKVPYRIVPGIC
Sbjct: 421 LYCEKVPYRIVPGIC 435

BLAST of Carg13881 vs. NCBI nr
Match: XP_022964233.1 (7-dehydrocholesterol reductase [Cucurbita moschata])

HSP 1 Score: 912.9 bits (2358), Expect = 1.0e-261
Identity = 430/435 (98.85%), Postives = 434/435 (99.77%), Query Frame = 0

Query: 1   MAEGSNTVHSPLVTYASMLSLLTLCPPFVIFLWYTMVHADGSVSQTMAYLYHNGLRGFVD 60
           MAEGSNTVHSPLVTYASMLSLLTLCPPFVIFLWYTMVHADGSVSQTMAYLYHNGLRG +D
Sbjct: 1   MAEGSNTVHSPLVTYASMLSLLTLCPPFVIFLWYTMVHADGSVSQTMAYLYHNGLRGLID 60

Query: 61  IWPPPSATAFKLIGSYAAFEAALQLLLPGKTVYGPISPTGNQPVYKANGVAAYVVTLVTY 120
           IWPPPSATAFKLIGSYAAFEAALQLLLPGKTVYGPISPTGNQPVYKANGVAAYVVTLVTY
Sbjct: 61  IWPPPSATAFKLIGSYAAFEAALQLLLPGKTVYGPISPTGNQPVYKANGVAAYVVTLVTY 120

Query: 121 IALWWFGIFNPTVVYDHLGEIFSALIIGSFIFCIVLYIKGCVAPSSSDSGSSGNIIIDFY 180
           IALWWFGIFNPTVVYDHLGEIFSALIIGSFIFCIVLYIKGCVAPSSSDSGSSGNIIIDFY
Sbjct: 121 IALWWFGIFNPTVVYDHLGEIFSALIIGSFIFCIVLYIKGCVAPSSSDSGSSGNIIIDFY 180

Query: 181 WGMELYPRIGKHFDIKVFTNCRFGMMSWAVLAVTYCIKQYEKNGTIADSMLVNTALMLAY 240
           WGMELYPRIGKHFDIKVFTNCRFGMMSWAVLAVTYCIKQYEKNGTIADSMLVNTALMLAY
Sbjct: 181 WGMELYPRIGKHFDIKVFTNCRFGMMSWAVLAVTYCIKQYEKNGTIADSMLVNTALMLAY 240

Query: 241 VTKFFWWEAGYWNTMDIAHDRAGFYICWGCLVWVPSVYTSPGMYLVNHPVHLGTQLAVYI 300
           VTKFFWWEAGYWNTMDIAHDRAGFYICWGCLVWVPSVYTSPGMYLVNHPV+LGTQLAVYI
Sbjct: 241 VTKFFWWEAGYWNTMDIAHDRAGFYICWGCLVWVPSVYTSPGMYLVNHPVNLGTQLAVYI 300

Query: 301 LVAGMLCIYINYDCDRQRQEFRRKNGKCLVWGKAPSKIVASYTTTSGETKTSLLLTSGWW 360
           LVAGMLCIYINYDCDRQRQEFRRKNGKCLVWGKAPSKIVASYTTTSGETKTSLLLTSGWW
Sbjct: 301 LVAGMLCIYINYDCDRQRQEFRRKNGKCLVWGKAPSKIVASYTTTSGETKTSLLLTSGWW 360

Query: 361 GLARHFHYVPEILAAFFWTVPALFSHFLPYFYVIFLTILLVDRAKRDDDRCRSKYGKYWK 420
           GLARHFHYVPEILAAFFWTVPALF+HFLPYFYVIFLTILLVDRAKRDDDRCRSKYGKYWK
Sbjct: 361 GLARHFHYVPEILAAFFWTVPALFNHFLPYFYVIFLTILLVDRAKRDDDRCRSKYGKYWK 420

Query: 421 LYCEKVPYRIVPGIC 436
           LYCEKVPYRI+PGIC
Sbjct: 421 LYCEKVPYRIIPGIC 435

BLAST of Carg13881 vs. NCBI nr
Match: XP_023513993.1 (7-dehydrocholesterol reductase [Cucurbita pepo subsp. pepo])

HSP 1 Score: 904.8 bits (2337), Expect = 2.8e-259
Identity = 428/435 (98.39%), Postives = 432/435 (99.31%), Query Frame = 0

Query: 1   MAEGSNTVHSPLVTYASMLSLLTLCPPFVIFLWYTMVHADGSVSQTMAYLYHNGLRGFVD 60
           MAEGSNTVHSPLVTYASMLSLLTLCPPFVIFLWYTMVHADGSVSQTMAYLY NGLRGF+D
Sbjct: 1   MAEGSNTVHSPLVTYASMLSLLTLCPPFVIFLWYTMVHADGSVSQTMAYLYRNGLRGFID 60

Query: 61  IWPPPSATAFKLIGSYAAFEAALQLLLPGKTVYGPISPTGNQPVYKANGVAAYVVTLVTY 120
           IWPPPSATAFKLIGSYAAFEAALQLLLPGKTVYGPISPTGNQPVYKANGVAAYVVTLVTY
Sbjct: 61  IWPPPSATAFKLIGSYAAFEAALQLLLPGKTVYGPISPTGNQPVYKANGVAAYVVTLVTY 120

Query: 121 IALWWFGIFNPTVVYDHLGEIFSALIIGSFIFCIVLYIKGCVAPSSSDSGSSGNIIIDFY 180
           IALW FGIFNPTVVYDHLGEIFSALIIGSFIFCIVLYIKGCVAPSSSDSGSSGNIIIDFY
Sbjct: 121 IALWRFGIFNPTVVYDHLGEIFSALIIGSFIFCIVLYIKGCVAPSSSDSGSSGNIIIDFY 180

Query: 181 WGMELYPRIGKHFDIKVFTNCRFGMMSWAVLAVTYCIKQYEKNGTIADSMLVNTALMLAY 240
           WGMELYPRIGKHFDIKVFTNCRFGMMSWAVLAVTYCIKQYEKNGTIADSMLVNTALMLAY
Sbjct: 181 WGMELYPRIGKHFDIKVFTNCRFGMMSWAVLAVTYCIKQYEKNGTIADSMLVNTALMLAY 240

Query: 241 VTKFFWWEAGYWNTMDIAHDRAGFYICWGCLVWVPSVYTSPGMYLVNHPVHLGTQLAVYI 300
           VTKFFWWEAGYWNTMDIAHDRAGFYICWGCLVWVPSVYTSPGMYLVNHPV+LGTQLAVYI
Sbjct: 241 VTKFFWWEAGYWNTMDIAHDRAGFYICWGCLVWVPSVYTSPGMYLVNHPVNLGTQLAVYI 300

Query: 301 LVAGMLCIYINYDCDRQRQEFRRKNGKCLVWGKAPSKIVASYTTTSGETKTSLLLTSGWW 360
           LVAGMLCIYINYDCDRQRQEFRRKNGKCLVWGKAPSKIVASYTT SGETKTSLLLTSGWW
Sbjct: 301 LVAGMLCIYINYDCDRQRQEFRRKNGKCLVWGKAPSKIVASYTTASGETKTSLLLTSGWW 360

Query: 361 GLARHFHYVPEILAAFFWTVPALFSHFLPYFYVIFLTILLVDRAKRDDDRCRSKYGKYWK 420
           GLARHFHYVPEILAAFFWTVPALF+HFLPYFYVIFLTILLVDRAKRDDDRCRSKYGKYWK
Sbjct: 361 GLARHFHYVPEILAAFFWTVPALFNHFLPYFYVIFLTILLVDRAKRDDDRCRSKYGKYWK 420

Query: 421 LYCEKVPYRIVPGIC 436
           LYCEKVPYRI+PGIC
Sbjct: 421 LYCEKVPYRIIPGIC 435

BLAST of Carg13881 vs. NCBI nr
Match: XP_022999960.1 (7-dehydrocholesterol reductase [Cucurbita maxima])

HSP 1 Score: 900.2 bits (2325), Expect = 7.0e-258
Identity = 425/435 (97.70%), Postives = 431/435 (99.08%), Query Frame = 0

Query: 1   MAEGSNTVHSPLVTYASMLSLLTLCPPFVIFLWYTMVHADGSVSQTMAYLYHNGLRGFVD 60
           MAEGSNTVHSPLVTYASMLSLLTLCPPFVIFLWYTMVHADGSVSQTMAYLYHNGLRGF+D
Sbjct: 1   MAEGSNTVHSPLVTYASMLSLLTLCPPFVIFLWYTMVHADGSVSQTMAYLYHNGLRGFID 60

Query: 61  IWPPPSATAFKLIGSYAAFEAALQLLLPGKTVYGPISPTGNQPVYKANGVAAYVVTLVTY 120
           IWPPPSATAFKLIGSYAAFEAALQLLLPGKTVYGPISPTGNQPVYKANGVAAYVVTLVTY
Sbjct: 61  IWPPPSATAFKLIGSYAAFEAALQLLLPGKTVYGPISPTGNQPVYKANGVAAYVVTLVTY 120

Query: 121 IALWWFGIFNPTVVYDHLGEIFSALIIGSFIFCIVLYIKGCVAPSSSDSGSSGNIIIDFY 180
           IALW FGIFNPTVVYDHLGEIFS LIIGSFIFCIVLYIKGCVAPSSSDSGSSG+IIIDFY
Sbjct: 121 IALWRFGIFNPTVVYDHLGEIFSTLIIGSFIFCIVLYIKGCVAPSSSDSGSSGDIIIDFY 180

Query: 181 WGMELYPRIGKHFDIKVFTNCRFGMMSWAVLAVTYCIKQYEKNGTIADSMLVNTALMLAY 240
           WGMELYPRIGKHFDIKVFTNCRFGMMSWAVLAVTYCI QYEKNGTIADSMLVNTALMLAY
Sbjct: 181 WGMELYPRIGKHFDIKVFTNCRFGMMSWAVLAVTYCINQYEKNGTIADSMLVNTALMLAY 240

Query: 241 VTKFFWWEAGYWNTMDIAHDRAGFYICWGCLVWVPSVYTSPGMYLVNHPVHLGTQLAVYI 300
           VTKFFWWEAGYWNTMDIAHDRAGFYICWGCLVWVPSVYTSPGMYLVNHPV+LG QLAVYI
Sbjct: 241 VTKFFWWEAGYWNTMDIAHDRAGFYICWGCLVWVPSVYTSPGMYLVNHPVNLGIQLAVYI 300

Query: 301 LVAGMLCIYINYDCDRQRQEFRRKNGKCLVWGKAPSKIVASYTTTSGETKTSLLLTSGWW 360
           LVAGMLCIYINYDCDRQRQEFRRKNGKCLVWGKAPSKIVASYTTTSGETKTSLLLTSGWW
Sbjct: 301 LVAGMLCIYINYDCDRQRQEFRRKNGKCLVWGKAPSKIVASYTTTSGETKTSLLLTSGWW 360

Query: 361 GLARHFHYVPEILAAFFWTVPALFSHFLPYFYVIFLTILLVDRAKRDDDRCRSKYGKYWK 420
           GLARHFHYVPEILAAFFWTVPALF+HFLPYFYVIFLTILLVDRAKRDD+RCRSKYGKYWK
Sbjct: 361 GLARHFHYVPEILAAFFWTVPALFNHFLPYFYVIFLTILLVDRAKRDDNRCRSKYGKYWK 420

Query: 421 LYCEKVPYRIVPGIC 436
           LYCEKVPYRI+PGIC
Sbjct: 421 LYCEKVPYRIIPGIC 435

BLAST of Carg13881 vs. ExPASy Swiss-Prot
Match: Q9LDU6 (7-dehydrocholesterol reductase OS=Arabidopsis thaliana OX=3702 GN=DWF5 PE=1 SV=1)

HSP 1 Score: 790.0 bits (2039), Expect = 1.3e-227
Identity = 364/430 (84.65%), Postives = 392/430 (91.16%), Query Frame = 0

Query: 5   SNTVHSPLVTYASMLSLLTLCPPFVIFLWYTMVHADGSVSQTMAYLYHNGLRGFVDIWPP 64
           + TVHSP+VTYASMLSLL  CPPFVI LWYTMVH DGSV+QT  + + NG++G ++IWP 
Sbjct: 2   AETVHSPIVTYASMLSLLAFCPPFVILLWYTMVHQDGSVTQTFGFFWENGVQGLINIWPR 61

Query: 65  PSATAFKLIGSYAAFEAALQLLLPGKTVYGPISPTGNQPVYKANGVAAYVVTLVTYIALW 124
           P+  A+K+I  Y AFEA LQLLLPGK V GPISP GN+PVYKANG+AAY VTL TY+ LW
Sbjct: 62  PTLIAWKIIFCYGAFEAILQLLLPGKRVEGPISPAGNRPVYKANGLAAYFVTLATYLGLW 121

Query: 125 WFGIFNPTVVYDHLGEIFSALIIGSFIFCIVLYIKGCVAPSSSDSGSSGNIIIDFYWGME 184
           WFGIFNP +VYDHLGEIFSALI GSFIFC++LYIKG VAPSSSDSGS GN+IIDFYWGME
Sbjct: 122 WFGIFNPAIVYDHLGEIFSALIFGSFIFCVLLYIKGHVAPSSSDSGSCGNLIIDFYWGME 181

Query: 185 LYPRIGKHFDIKVFTNCRFGMMSWAVLAVTYCIKQYEKNGTIADSMLVNTALMLAYVTKF 244
           LYPRIGK FDIKVFTNCRFGMMSWAVLAVTYCIKQYE NG ++DSMLVNT LML YVTKF
Sbjct: 182 LYPRIGKSFDIKVFTNCRFGMMSWAVLAVTYCIKQYEINGKVSDSMLVNTILMLVYVTKF 241

Query: 245 FWWEAGYWNTMDIAHDRAGFYICWGCLVWVPSVYTSPGMYLVNHPVHLGTQLAVYILVAG 304
           FWWEAGYWNTMDIAHDRAGFYICWGCLVWVPSVYTSPGMYLVNHPV LGTQLA+YILVAG
Sbjct: 242 FWWEAGYWNTMDIAHDRAGFYICWGCLVWVPSVYTSPGMYLVNHPVELGTQLAIYILVAG 301

Query: 305 MLCIYINYDCDRQRQEFRRKNGKCLVWGKAPSKIVASYTTTSGETKTSLLLTSGWWGLAR 364
           +LCIYINYDCDRQRQEFRR NGKCLVWG+APSKIVASYTTTSGETKTSLLLTSGWWGLAR
Sbjct: 302 ILCIYINYDCDRQRQEFRRTNGKCLVWGRAPSKIVASYTTTSGETKTSLLLTSGWWGLAR 361

Query: 365 HFHYVPEILAAFFWTVPALFSHFLPYFYVIFLTILLVDRAKRDDDRCRSKYGKYWKLYCE 424
           HFHYVPEIL+AFFWTVPALF +FL YFYVIFLT+LL DRAKRDDDRCRSKYGKYWKLYCE
Sbjct: 362 HFHYVPEILSAFFWTVPALFDNFLAYFYVIFLTLLLFDRAKRDDDRCRSKYGKYWKLYCE 421

Query: 425 KVPYRIVPGI 435
           KV YRI+PGI
Sbjct: 422 KVKYRIIPGI 431

BLAST of Carg13881 vs. ExPASy Swiss-Prot
Match: O88455 (7-dehydrocholesterol reductase OS=Mus musculus OX=10090 GN=Dhcr7 PE=1 SV=1)

HSP 1 Score: 293.5 bits (750), Expect = 3.9e-78
Identity = 164/437 (37.53%), Postives = 252/437 (57.67%), Query Frame = 0

Query: 16  ASMLSLLTLCPPFVIFLWYTMVHADGSVSQTMAYL-YHNGLRGFVDIW---PPPSATAFK 75
           AS++ LL L  PF+++ ++ M     S S T   L    G     DIW   PP +A A +
Sbjct: 37  ASIIFLL-LFAPFIVY-YFIMACDQYSCSLTAPALDIATGHASLADIWAKTPPVTAKAAQ 96

Query: 76  LIGSYAAFEAALQLLLP-----------GKTVYGPISPTGNQPVYKANGVAAYVVT-LVT 135
           L   + +F+  L   LP           G    G I+P G    Y+ NG+ A+++T ++ 
Sbjct: 97  LYALWVSFQVLLYSWLPDFCHRFLPGYVGGVQEGAITPAGVVNKYEVNGLQAWLITHILW 156

Query: 136 YIALWWFGIFNPTVVYDH-LGEIFSALIIGSFIFCIVLYIKGCVAPSSS-DSGSSGNIII 195
           ++  +    F+PT+++D+ +  ++ A I+G  +    + IKG + P+S+ D   +GN   
Sbjct: 157 FVNAYLLSWFSPTIIFDNWIPLLWCANILGYAVSTFAM-IKGYLFPTSAEDCKFTGNFFY 216

Query: 196 DFYWGMELYPRIGKHFDIKVFTNCRFGMMSWAVLAVTYCIKQYEKNGTIADSMLVNTALM 255
           ++  G+E  PRIGK FD K+F N R G+++W ++ +++  KQ E  G + +SM++   L 
Sbjct: 217 NYMMGIEFNPRIGKWFDFKLFFNGRPGIVAWTLINLSFAAKQQELYGHVTNSMILVNVLQ 276

Query: 256 LAYVTKFFWWEAGYWNTMDIAHDRAGFYICWGCLVWVPSVYTSPGMYLVNHPVHLGTQLA 315
             YV  FFW E  Y  T+DI HD  G+Y+ WG  VW+P +YT  G+YLV HPV L T  A
Sbjct: 277 AIYVLDFFWNETWYLKTIDICHDHFGWYLGWGDCVWLPYLYTLQGLYLVYHPVQLSTPNA 336

Query: 316 VYILVAGMLCIYINYDCDRQRQEFRRKNGKCLVWGKAPSKIVASYTTTSGETKTSLLLTS 375
           + IL+ G++  YI    + Q+  FRR +G+CL+WGK P  I  SYT+  G    S LL S
Sbjct: 337 LGILLLGLVGYYIFRMTNHQKDLFRRTDGRCLIWGKKPKAIECSYTSADGLKHHSKLLVS 396

Query: 376 GWWGLARHFHYVPEILAAFFWTVPALFSHFLPYFYVIFLTILLVDRAKRDDDRCRSKYGK 435
           G+WG+ARHF+Y  +++ +  + +     H LPYFY+I++TILL  R  RD+ RC +KYG+
Sbjct: 397 GFWGVARHFNYTGDLMGSLAYCLACGGGHLLPYFYIIYMTILLTHRCLRDEHRCANKYGR 456

BLAST of Carg13881 vs. ExPASy Swiss-Prot
Match: Q9Z2Z8 (7-dehydrocholesterol reductase OS=Rattus norvegicus OX=10116 GN=Dhcr7 PE=1 SV=1)

HSP 1 Score: 291.6 bits (745), Expect = 1.5e-77
Identity = 163/442 (36.88%), Postives = 252/442 (57.01%), Query Frame = 0

Query: 13  VTYASMLSL--LTLCPPFVIFLWYTMVHADGSVSQTMAYL-YHNGLRGFVDIW---PPPS 72
           V + S++S+  L L  PF+++ ++ M     S S T   L    G     DIW   PP +
Sbjct: 31  VDWFSLVSVIFLLLFAPFIVY-YFIMACDQYSCSLTAPILDVATGRASLADIWAKTPPVT 90

Query: 73  ATAFKLIGSYAAFEAALQLLLP-----------GKTVYGPISPTGNQPVYKANGVAAYVV 132
           A A +L   + +F+  L   LP           G    G I+P G    Y+ NG+ A+++
Sbjct: 91  AKAAQLYALWVSFQVLLYSWLPDFCHRFLPGYVGGVQEGAITPAGIVNKYEVNGLQAWLI 150

Query: 133 T-LVTYIALWWFGIFNPTVVYDH-LGEIFSALIIGSFIFCIVLYIKGCVAPSSS-DSGSS 192
           T  + ++  +    F+PT+++D+ +  ++ A I+G  +    + IKG + P+S+ D   +
Sbjct: 151 THFLWFVNAYLLSWFSPTIIFDNWIPLLWCANILGYAVSTFAM-IKGYLFPTSAEDCKFT 210

Query: 193 GNIIIDFYWGMELYPRIGKHFDIKVFTNCRFGMMSWAVLAVTYCIKQYEKNGTIADSMLV 252
           GN   ++  G+E  PRIGK FD K+F N R G+++W ++ +++  KQ E  G + +SM++
Sbjct: 211 GNFFYNYMMGIEFNPRIGKWFDFKLFFNGRPGIVAWTLINLSFAAKQQELYGHVTNSMIL 270

Query: 253 NTALMLAYVTKFFWWEAGYWNTMDIAHDRAGFYICWGCLVWVPSVYTSPGMYLVNHPVHL 312
              L   YV  FFW E  Y  T+DI HD  G+Y+ WG  VW+P +YT  G+YLV HPV L
Sbjct: 271 VNVLQAIYVLDFFWNETWYLKTIDICHDHFGWYLGWGDCVWLPYLYTLQGLYLVYHPVQL 330

Query: 313 GTQLAVYILVAGMLCIYINYDCDRQRQEFRRKNGKCLVWGKAPSKIVASYTTTSGETKTS 372
            T  A+ +L+ G++  YI    + Q+  FRR +G CL+WGK P  I  SYT+  G    S
Sbjct: 331 STPNALGVLLLGLVGYYIFRMTNHQKDLFRRTDGHCLIWGKKPKAIECSYTSADGLKHRS 390

Query: 373 LLLTSGWWGLARHFHYVPEILAAFFWTVPALFSHFLPYFYVIFLTILLVDRAKRDDDRCR 432
            LL SG+WG+ARHF+Y  +++ +  + +     H LPYFY+I++TILL  R  RD+ RC 
Sbjct: 391 KLLVSGFWGVARHFNYTGDLMGSLAYCLACGGGHLLPYFYIIYMTILLTHRCLRDEHRCA 450

Query: 433 SKYGKYWKLYCEKVPYRIVPGI 435
           +KYG+ W+ Y   VPYR++PGI
Sbjct: 451 NKYGRDWERYVAAVPYRLLPGI 470

BLAST of Carg13881 vs. ExPASy Swiss-Prot
Match: Q7ZXH1 (7-dehydrocholesterol reductase OS=Xenopus laevis OX=8355 GN=dhcr7 PE=2 SV=1)

HSP 1 Score: 290.0 bits (741), Expect = 4.3e-77
Identity = 158/435 (36.32%), Postives = 242/435 (55.63%), Query Frame = 0

Query: 16  ASMLSLLTLCPPFVIFLWYTMVHADGSVSQTMAYLYHNGLRGFVDIW---PPPSATAFKL 75
           AS++ LL   P  V +   +      +++  +  LY +G     DIW   P  + TA K+
Sbjct: 39  ASVIFLLAFAPLIVYYFVMSCDQYQCALTAPVLDLY-SGKARLSDIWDKTPALTWTAVKI 98

Query: 76  IGSYAAFEAALQLLLP-----------GKTVYGPISPTGNQPVYKANGVAAYVVT-LVTY 135
             ++ +F+  L + LP           G    G  +P G    Y+ NG+ A+ +T L+ +
Sbjct: 99  YLAWVSFQVFLYMFLPDILHKFVPGYEGGVQEGARTPAGLINKYQVNGLQAWTITHLLWF 158

Query: 136 IALWWFGIFNPTVVYDH-LGEIFSALIIGSFIFCIVLYIKGCVAPSSSDSGSSGNIIIDF 195
              + F  F+PT+V D+ +  ++ A ++G  +    L        +++D   +GN   D+
Sbjct: 159 ANAYHFHWFSPTIVIDNWIPLLWCANLLGYSVATFALVKANFFPTNANDCKFTGNFFYDY 218

Query: 196 YWGMELYPRIGKHFDIKVFTNCRFGMMSWAVLAVTYCIKQYEKNGTIADSMLVNTALMLA 255
             G+E  PRIGK FD K+F N R G+++W ++ ++Y  KQ E  G + +SM++   L   
Sbjct: 219 MMGIEFNPRIGKWFDFKLFFNGRPGIVAWTLINLSYAAKQQELYGQVTNSMILVNVLQAI 278

Query: 256 YVTKFFWWEAGYWNTMDIAHDRAGFYICWGCLVWVPSVYTSPGMYLVNHPVHLGTQLAVY 315
           YV  FFW E+ Y  T+DI HD  G+Y+ WG  VW+P +YT  G+YLV +PV L T  AV 
Sbjct: 279 YVVDFFWNESWYLKTIDICHDHFGWYLGWGDCVWLPYLYTLQGLYLVYNPVELSTTAAVA 338

Query: 316 ILVAGMLCIYINYDCDRQRQEFRRKNGKCLVWGKAPSKIVASYTTTSGETKTSLLLTSGW 375
           +L+ G++  YI    + Q+  FRR NG C +WGK P  I   Y +  G+   S L+ SG+
Sbjct: 339 VLLLGLIGYYIFRMTNHQKDLFRRTNGNCKIWGKKPKSIECFYVSADGKRHYSKLMISGF 398

Query: 376 WGLARHFHYVPEILAAFFWTVPALFSHFLPYFYVIFLTILLVDRAKRDDDRCRSKYGKYW 435
           WG+ARH +Y  +++ +  + +   F H LPYFY I++TILLV R  RD+ RC SKYGK W
Sbjct: 399 WGVARHLNYTGDLMGSLAYCLACGFDHLLPYFYFIYMTILLVHRCIRDEHRCSSKYGKDW 458

BLAST of Carg13881 vs. ExPASy Swiss-Prot
Match: Q5E9J5 (7-dehydrocholesterol reductase OS=Bos taurus OX=9913 GN=DHCR7 PE=2 SV=1)

HSP 1 Score: 287.0 bits (733), Expect = 3.7e-76
Identity = 156/436 (35.78%), Postives = 246/436 (56.42%), Query Frame = 0

Query: 16  ASMLSLLTLCPPFVIFLWYTMVHADGSVSQTMAYLYHNGLRGFVDIW---PPPSATAFKL 75
           AS++ LL L  PF+++ +       G            G     DIW   PP +A A ++
Sbjct: 41  ASVIFLL-LFAPFIVYYFIMACDQYGCSLTVPVADLATGRARLADIWARTPPVTAKAAQI 100

Query: 76  IGSYAAFEAALQLLLP-----------GKTVYGPISPTGNQPVYKANGVAAYVVT-LVTY 135
             ++   +  L +LLP           G    G ++P G    Y+ NG+ A+++T L+ +
Sbjct: 101 YTAWVTLQVLLYMLLPDFCHKFLPGYVGGVQEGAVTPAGAVNKYEINGLQAWLLTHLLWF 160

Query: 136 IALWWFGIFNPTVVYDH-LGEIFSALIIGSFIFCIVLYIKGCVAPSSS-DSGSSGNIIID 195
                 G F+PT+++D+ +  ++ A I+G  +    + +KG + P+ + +   +GN   +
Sbjct: 161 ANAHLLGWFSPTIIFDNWIPLLWCANILGYTVSTFAM-VKGYLFPTDARECKFTGNFFYN 220

Query: 196 FYWGMELYPRIGKHFDIKVFTNCRFGMMSWAVLAVTYCIKQYEKNGTIADSMLVNTALML 255
           +  G+E  PRIGK FD K+F N R G+++W ++ +++  KQ E  G + +SM++   L  
Sbjct: 221 YMMGVEFNPRIGKWFDFKLFFNGRPGIVAWTLINLSFAAKQQELYGHVTNSMVLVNILQA 280

Query: 256 AYVTKFFWWEAGYWNTMDIAHDRAGFYICWGCLVWVPSVYTSPGMYLVNHPVHLGTQLAV 315
            YV  FFW E  Y  T+DI HD  G+Y+ WG  VW+P +YT  G+YLV HPV L T  A+
Sbjct: 281 IYVLDFFWNETWYLKTIDICHDHFGWYLGWGDCVWLPYLYTLQGLYLVYHPVQLPTYYAL 340

Query: 316 YILVAGMLCIYINYDCDRQRQEFRRKNGKCLVWGKAPSKIVASYTTTSGETKTSLLLTSG 375
            +L+ G+L  YI    + Q+  FRR +G+CL+WG+ P  I  SYT+  G+   S LL SG
Sbjct: 341 GVLLLGLLGYYIFRMTNHQKDLFRRTDGRCLIWGRKPKAIECSYTSADGQRHHSKLLVSG 400

Query: 376 WWGLARHFHYVPEILAAFFWTVPALFSHFLPYFYVIFLTILLVDRAKRDDDRCRSKYGKY 435
           +WG+ARHF+Y  +++ +  + +     H LPYFY+IF+ ILL  R  RD+ RC +KYG+ 
Sbjct: 401 FWGVARHFNYTGDLMGSLAYCLACGGGHLLPYFYIIFMAILLTHRCLRDEHRCANKYGRD 460

BLAST of Carg13881 vs. ExPASy TrEMBL
Match: A0A6J1HK80 (7-dehydrocholesterol reductase OS=Cucurbita moschata OX=3662 GN=LOC111464317 PE=3 SV=1)

HSP 1 Score: 912.9 bits (2358), Expect = 5.0e-262
Identity = 430/435 (98.85%), Postives = 434/435 (99.77%), Query Frame = 0

Query: 1   MAEGSNTVHSPLVTYASMLSLLTLCPPFVIFLWYTMVHADGSVSQTMAYLYHNGLRGFVD 60
           MAEGSNTVHSPLVTYASMLSLLTLCPPFVIFLWYTMVHADGSVSQTMAYLYHNGLRG +D
Sbjct: 1   MAEGSNTVHSPLVTYASMLSLLTLCPPFVIFLWYTMVHADGSVSQTMAYLYHNGLRGLID 60

Query: 61  IWPPPSATAFKLIGSYAAFEAALQLLLPGKTVYGPISPTGNQPVYKANGVAAYVVTLVTY 120
           IWPPPSATAFKLIGSYAAFEAALQLLLPGKTVYGPISPTGNQPVYKANGVAAYVVTLVTY
Sbjct: 61  IWPPPSATAFKLIGSYAAFEAALQLLLPGKTVYGPISPTGNQPVYKANGVAAYVVTLVTY 120

Query: 121 IALWWFGIFNPTVVYDHLGEIFSALIIGSFIFCIVLYIKGCVAPSSSDSGSSGNIIIDFY 180
           IALWWFGIFNPTVVYDHLGEIFSALIIGSFIFCIVLYIKGCVAPSSSDSGSSGNIIIDFY
Sbjct: 121 IALWWFGIFNPTVVYDHLGEIFSALIIGSFIFCIVLYIKGCVAPSSSDSGSSGNIIIDFY 180

Query: 181 WGMELYPRIGKHFDIKVFTNCRFGMMSWAVLAVTYCIKQYEKNGTIADSMLVNTALMLAY 240
           WGMELYPRIGKHFDIKVFTNCRFGMMSWAVLAVTYCIKQYEKNGTIADSMLVNTALMLAY
Sbjct: 181 WGMELYPRIGKHFDIKVFTNCRFGMMSWAVLAVTYCIKQYEKNGTIADSMLVNTALMLAY 240

Query: 241 VTKFFWWEAGYWNTMDIAHDRAGFYICWGCLVWVPSVYTSPGMYLVNHPVHLGTQLAVYI 300
           VTKFFWWEAGYWNTMDIAHDRAGFYICWGCLVWVPSVYTSPGMYLVNHPV+LGTQLAVYI
Sbjct: 241 VTKFFWWEAGYWNTMDIAHDRAGFYICWGCLVWVPSVYTSPGMYLVNHPVNLGTQLAVYI 300

Query: 301 LVAGMLCIYINYDCDRQRQEFRRKNGKCLVWGKAPSKIVASYTTTSGETKTSLLLTSGWW 360
           LVAGMLCIYINYDCDRQRQEFRRKNGKCLVWGKAPSKIVASYTTTSGETKTSLLLTSGWW
Sbjct: 301 LVAGMLCIYINYDCDRQRQEFRRKNGKCLVWGKAPSKIVASYTTTSGETKTSLLLTSGWW 360

Query: 361 GLARHFHYVPEILAAFFWTVPALFSHFLPYFYVIFLTILLVDRAKRDDDRCRSKYGKYWK 420
           GLARHFHYVPEILAAFFWTVPALF+HFLPYFYVIFLTILLVDRAKRDDDRCRSKYGKYWK
Sbjct: 361 GLARHFHYVPEILAAFFWTVPALFNHFLPYFYVIFLTILLVDRAKRDDDRCRSKYGKYWK 420

Query: 421 LYCEKVPYRIVPGIC 436
           LYCEKVPYRI+PGIC
Sbjct: 421 LYCEKVPYRIIPGIC 435

BLAST of Carg13881 vs. ExPASy TrEMBL
Match: A0A6J1KH14 (7-dehydrocholesterol reductase OS=Cucurbita maxima OX=3661 GN=LOC111494288 PE=3 SV=1)

HSP 1 Score: 900.2 bits (2325), Expect = 3.4e-258
Identity = 425/435 (97.70%), Postives = 431/435 (99.08%), Query Frame = 0

Query: 1   MAEGSNTVHSPLVTYASMLSLLTLCPPFVIFLWYTMVHADGSVSQTMAYLYHNGLRGFVD 60
           MAEGSNTVHSPLVTYASMLSLLTLCPPFVIFLWYTMVHADGSVSQTMAYLYHNGLRGF+D
Sbjct: 1   MAEGSNTVHSPLVTYASMLSLLTLCPPFVIFLWYTMVHADGSVSQTMAYLYHNGLRGFID 60

Query: 61  IWPPPSATAFKLIGSYAAFEAALQLLLPGKTVYGPISPTGNQPVYKANGVAAYVVTLVTY 120
           IWPPPSATAFKLIGSYAAFEAALQLLLPGKTVYGPISPTGNQPVYKANGVAAYVVTLVTY
Sbjct: 61  IWPPPSATAFKLIGSYAAFEAALQLLLPGKTVYGPISPTGNQPVYKANGVAAYVVTLVTY 120

Query: 121 IALWWFGIFNPTVVYDHLGEIFSALIIGSFIFCIVLYIKGCVAPSSSDSGSSGNIIIDFY 180
           IALW FGIFNPTVVYDHLGEIFS LIIGSFIFCIVLYIKGCVAPSSSDSGSSG+IIIDFY
Sbjct: 121 IALWRFGIFNPTVVYDHLGEIFSTLIIGSFIFCIVLYIKGCVAPSSSDSGSSGDIIIDFY 180

Query: 181 WGMELYPRIGKHFDIKVFTNCRFGMMSWAVLAVTYCIKQYEKNGTIADSMLVNTALMLAY 240
           WGMELYPRIGKHFDIKVFTNCRFGMMSWAVLAVTYCI QYEKNGTIADSMLVNTALMLAY
Sbjct: 181 WGMELYPRIGKHFDIKVFTNCRFGMMSWAVLAVTYCINQYEKNGTIADSMLVNTALMLAY 240

Query: 241 VTKFFWWEAGYWNTMDIAHDRAGFYICWGCLVWVPSVYTSPGMYLVNHPVHLGTQLAVYI 300
           VTKFFWWEAGYWNTMDIAHDRAGFYICWGCLVWVPSVYTSPGMYLVNHPV+LG QLAVYI
Sbjct: 241 VTKFFWWEAGYWNTMDIAHDRAGFYICWGCLVWVPSVYTSPGMYLVNHPVNLGIQLAVYI 300

Query: 301 LVAGMLCIYINYDCDRQRQEFRRKNGKCLVWGKAPSKIVASYTTTSGETKTSLLLTSGWW 360
           LVAGMLCIYINYDCDRQRQEFRRKNGKCLVWGKAPSKIVASYTTTSGETKTSLLLTSGWW
Sbjct: 301 LVAGMLCIYINYDCDRQRQEFRRKNGKCLVWGKAPSKIVASYTTTSGETKTSLLLTSGWW 360

Query: 361 GLARHFHYVPEILAAFFWTVPALFSHFLPYFYVIFLTILLVDRAKRDDDRCRSKYGKYWK 420
           GLARHFHYVPEILAAFFWTVPALF+HFLPYFYVIFLTILLVDRAKRDD+RCRSKYGKYWK
Sbjct: 361 GLARHFHYVPEILAAFFWTVPALFNHFLPYFYVIFLTILLVDRAKRDDNRCRSKYGKYWK 420

Query: 421 LYCEKVPYRIVPGIC 436
           LYCEKVPYRI+PGIC
Sbjct: 421 LYCEKVPYRIIPGIC 435

BLAST of Carg13881 vs. ExPASy TrEMBL
Match: A0A6J1D3V8 (7-dehydrocholesterol reductase OS=Momordica charantia OX=3673 GN=LOC111016744 PE=3 SV=1)

HSP 1 Score: 854.0 bits (2205), Expect = 2.8e-244
Identity = 398/434 (91.71%), Postives = 416/434 (95.85%), Query Frame = 0

Query: 1   MAEGSNTVHSPLVTYASMLSLLTLCPPFVIFLWYTMVHADGSVSQTMAYLYHNGLRGFVD 60
           MAEGS TVHSP++TY SMLSLL +CPPFVIFLWYTMVHADGSVSQT  YLYHNG+RGF++
Sbjct: 1   MAEGSTTVHSPILTYVSMLSLLIICPPFVIFLWYTMVHADGSVSQTWNYLYHNGVRGFMN 60

Query: 61  IWPPPSATAFKLIGSYAAFEAALQLLLPGKTVYGPISPTGNQPVYKANGVAAYVVTLVTY 120
           IWPPPSATA K+IG YAAFEAALQLLLPG  V GP+SPTGNQPVYKANGVAAY+VTLVTY
Sbjct: 61  IWPPPSATACKIIGCYAAFEAALQLLLPGNRVQGPVSPTGNQPVYKANGVAAYIVTLVTY 120

Query: 121 IALWWFGIFNPTVVYDHLGEIFSALIIGSFIFCIVLYIKGCVAPSSSDSGSSGNIIIDFY 180
           IALWWFGIFNPT+VYDHLGEIFSALI GS IFCI LYIKG VAPSSSDSGSSGNIIIDFY
Sbjct: 121 IALWWFGIFNPTIVYDHLGEIFSALIFGSLIFCIFLYIKGHVAPSSSDSGSSGNIIIDFY 180

Query: 181 WGMELYPRIGKHFDIKVFTNCRFGMMSWAVLAVTYCIKQYEKNGTIADSMLVNTALMLAY 240
           WGMELYPRIGK+FDIKVFTNCRFGMMSWAVLAVTYCIKQYEKNGTIADSMLVNTALML Y
Sbjct: 181 WGMELYPRIGKNFDIKVFTNCRFGMMSWAVLAVTYCIKQYEKNGTIADSMLVNTALMLVY 240

Query: 241 VTKFFWWEAGYWNTMDIAHDRAGFYICWGCLVWVPSVYTSPGMYLVNHPVHLGTQLAVYI 300
           VTKFFWWE+GYWNTMDIAHDRAGFYICWGCLVWVPSVYTSPGMYLVNHPV+LGTQLAVYI
Sbjct: 241 VTKFFWWESGYWNTMDIAHDRAGFYICWGCLVWVPSVYTSPGMYLVNHPVNLGTQLAVYI 300

Query: 301 LVAGMLCIYINYDCDRQRQEFRRKNGKCLVWGKAPSKIVASYTTTSGETKTSLLLTSGWW 360
           LVAG+LCIYINYDCDRQRQEFRRKNGKCL+W KAPSKIVASYTTTSGETKTSLLLTSGWW
Sbjct: 301 LVAGLLCIYINYDCDRQRQEFRRKNGKCLIWRKAPSKIVASYTTTSGETKTSLLLTSGWW 360

Query: 361 GLARHFHYVPEILAAFFWTVPALFSHFLPYFYVIFLTILLVDRAKRDDDRCRSKYGKYWK 420
           GLARHFHY PEILAAFFWTVPALF+HFLPYFYVIFLTILL+DRAKRDDDRCRSKYGKYWK
Sbjct: 361 GLARHFHYAPEILAAFFWTVPALFNHFLPYFYVIFLTILLLDRAKRDDDRCRSKYGKYWK 420

Query: 421 LYCEKVPYRIVPGI 435
           LYCEKV YRI+PGI
Sbjct: 421 LYCEKVAYRIIPGI 434

BLAST of Carg13881 vs. ExPASy TrEMBL
Match: A0A0A0KC98 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_7G447780 PE=3 SV=1)

HSP 1 Score: 839.0 bits (2166), Expect = 9.2e-240
Identity = 389/434 (89.63%), Postives = 412/434 (94.93%), Query Frame = 0

Query: 1   MAEGSNTVHSPLVTYASMLSLLTLCPPFVIFLWYTMVHADGSVSQTMAYLYHNGLRGFVD 60
           MAEGSNTVHSP +TY SMLSLLTLCPPFVI LWYTMVHADGSVSQT  YLY NG  GF++
Sbjct: 1   MAEGSNTVHSPFLTYVSMLSLLTLCPPFVILLWYTMVHADGSVSQTWNYLYQNGFEGFIN 60

Query: 61  IWPPPSATAFKLIGSYAAFEAALQLLLPGKTVYGPISPTGNQPVYKANGVAAYVVTLVTY 120
           IWP PSATA K+IG YAAFEAALQLLLPGK V GP+SPTGNQPVYKANGVAAY+VTL+TY
Sbjct: 61  IWPSPSATACKIIGCYAAFEAALQLLLPGKRVLGPVSPTGNQPVYKANGVAAYIVTLLTY 120

Query: 121 IALWWFGIFNPTVVYDHLGEIFSALIIGSFIFCIVLYIKGCVAPSSSDSGSSGNIIIDFY 180
           +ALW FGIF+P +VYDHLGEI+S LI GSFIFCI+LYIKG VAPSSSDSGSSGN+I+DFY
Sbjct: 121 VALWRFGIFDPAIVYDHLGEIYSTLIFGSFIFCILLYIKGHVAPSSSDSGSSGNVILDFY 180

Query: 181 WGMELYPRIGKHFDIKVFTNCRFGMMSWAVLAVTYCIKQYEKNGTIADSMLVNTALMLAY 240
           WGMELYPRIGKHFDIKVFTNCRFGMMSWAVLAVTYCIKQYEKNGT+ADS+LVNTALML Y
Sbjct: 181 WGMELYPRIGKHFDIKVFTNCRFGMMSWAVLAVTYCIKQYEKNGTLADSLLVNTALMLVY 240

Query: 241 VTKFFWWEAGYWNTMDIAHDRAGFYICWGCLVWVPSVYTSPGMYLVNHPVHLGTQLAVYI 300
           VTKFFWWEAGYWNTMDIAHDRAGFYICWGCLVWVPSVYTSPGMYLVNHPV+LGTQLAV I
Sbjct: 241 VTKFFWWEAGYWNTMDIAHDRAGFYICWGCLVWVPSVYTSPGMYLVNHPVNLGTQLAVSI 300

Query: 301 LVAGMLCIYINYDCDRQRQEFRRKNGKCLVWGKAPSKIVASYTTTSGETKTSLLLTSGWW 360
           LVAG+LCIYINYDCDRQRQEFRRKNGKCLVWGKAPSKIVA+YTTT+GET+TSLLLTSGWW
Sbjct: 301 LVAGILCIYINYDCDRQRQEFRRKNGKCLVWGKAPSKIVATYTTTTGETRTSLLLTSGWW 360

Query: 361 GLARHFHYVPEILAAFFWTVPALFSHFLPYFYVIFLTILLVDRAKRDDDRCRSKYGKYWK 420
           GLARHFHYVPEILAAFFWTVPALF HFLPYFYVIFLTILL DRAKRDDDRCRSKYGKYWK
Sbjct: 361 GLARHFHYVPEILAAFFWTVPALFHHFLPYFYVIFLTILLFDRAKRDDDRCRSKYGKYWK 420

Query: 421 LYCEKVPYRIVPGI 435
           LYCEKVPY+I+PGI
Sbjct: 421 LYCEKVPYKIIPGI 434

BLAST of Carg13881 vs. ExPASy TrEMBL
Match: A0A5A7UXH5 (7-dehydrocholesterol reductase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold347G001860 PE=3 SV=1)

HSP 1 Score: 838.2 bits (2164), Expect = 1.6e-239
Identity = 393/434 (90.55%), Postives = 410/434 (94.47%), Query Frame = 0

Query: 1   MAEGSNTVHSPLVTYASMLSLLTLCPPFVIFLWYTMVHADGSVSQTMAYLYHNGLRGFVD 60
           MAEGSNTVHSP +TY SMLSLLTLCPPFVI LWYTMVHADGSVSQT  YLY NG  GF++
Sbjct: 1   MAEGSNTVHSPFLTYVSMLSLLTLCPPFVILLWYTMVHADGSVSQTWNYLYQNGFEGFIN 60

Query: 61  IWPPPSATAFKLIGSYAAFEAALQLLLPGKTVYGPISPTGNQPVYKANGVAAYVVTLVTY 120
           IWP PSATA K+IG YAAFEAALQLLLPGK V GP+SPTGNQPVYKANGVAAY+VTLVTY
Sbjct: 61  IWPSPSATACKIIGCYAAFEAALQLLLPGKRVLGPVSPTGNQPVYKANGVAAYIVTLVTY 120

Query: 121 IALWWFGIFNPTVVYDHLGEIFSALIIGSFIFCIVLYIKGCVAPSSSDSGSSGNIIIDFY 180
           +ALW FGIF+P +VYDHLGEIFS LI GSFIFCI+LYIKG VAPSSSDSGSSGNII+DFY
Sbjct: 121 VALWRFGIFDPAIVYDHLGEIFSTLIFGSFIFCILLYIKGHVAPSSSDSGSSGNIILDFY 180

Query: 181 WGMELYPRIGKHFDIKVFTNCRFGMMSWAVLAVTYCIKQYEKNGTIADSMLVNTALMLAY 240
           WGMELYPRIGKHFDIKVFTNCRFGMMSWAVLAVTYCIKQYEKNG +ADSMLVNTALML Y
Sbjct: 181 WGMELYPRIGKHFDIKVFTNCRFGMMSWAVLAVTYCIKQYEKNGILADSMLVNTALMLVY 240

Query: 241 VTKFFWWEAGYWNTMDIAHDRAGFYICWGCLVWVPSVYTSPGMYLVNHPVHLGTQLAVYI 300
           VTKFFWWEAGYWNTMDIAHDRAGFYICWGCLVWVPSVYTSPGMYLVNHPV+LGTQLAV I
Sbjct: 241 VTKFFWWEAGYWNTMDIAHDRAGFYICWGCLVWVPSVYTSPGMYLVNHPVNLGTQLAVSI 300

Query: 301 LVAGMLCIYINYDCDRQRQEFRRKNGKCLVWGKAPSKIVASYTTTSGETKTSLLLTSGWW 360
           LVAG+LCIYINYDCDRQRQEFRRKNGK LVWGKAPSKIVASYTTTSGET+TSLLLTSGWW
Sbjct: 301 LVAGILCIYINYDCDRQRQEFRRKNGKRLVWGKAPSKIVASYTTTSGETRTSLLLTSGWW 360

Query: 361 GLARHFHYVPEILAAFFWTVPALFSHFLPYFYVIFLTILLVDRAKRDDDRCRSKYGKYWK 420
           GLARHFHYVPEILAAFFWTVPALF HFLPYFYVIFLTILL DRAKRDDDRCRSKYGKYWK
Sbjct: 361 GLARHFHYVPEILAAFFWTVPALFHHFLPYFYVIFLTILLFDRAKRDDDRCRSKYGKYWK 420

Query: 421 LYCEKVPYRIVPGI 435
           LYCEKVPY+I+PGI
Sbjct: 421 LYCEKVPYKIIPGI 434

BLAST of Carg13881 vs. TAIR 10
Match: AT1G50430.1 (Ergosterol biosynthesis ERG4/ERG24 family )

HSP 1 Score: 790.0 bits (2039), Expect = 9.5e-229
Identity = 364/430 (84.65%), Postives = 392/430 (91.16%), Query Frame = 0

Query: 5   SNTVHSPLVTYASMLSLLTLCPPFVIFLWYTMVHADGSVSQTMAYLYHNGLRGFVDIWPP 64
           + TVHSP+VTYASMLSLL  CPPFVI LWYTMVH DGSV+QT  + + NG++G ++IWP 
Sbjct: 2   AETVHSPIVTYASMLSLLAFCPPFVILLWYTMVHQDGSVTQTFGFFWENGVQGLINIWPR 61

Query: 65  PSATAFKLIGSYAAFEAALQLLLPGKTVYGPISPTGNQPVYKANGVAAYVVTLVTYIALW 124
           P+  A+K+I  Y AFEA LQLLLPGK V GPISP GN+PVYKANG+AAY VTL TY+ LW
Sbjct: 62  PTLIAWKIIFCYGAFEAILQLLLPGKRVEGPISPAGNRPVYKANGLAAYFVTLATYLGLW 121

Query: 125 WFGIFNPTVVYDHLGEIFSALIIGSFIFCIVLYIKGCVAPSSSDSGSSGNIIIDFYWGME 184
           WFGIFNP +VYDHLGEIFSALI GSFIFC++LYIKG VAPSSSDSGS GN+IIDFYWGME
Sbjct: 122 WFGIFNPAIVYDHLGEIFSALIFGSFIFCVLLYIKGHVAPSSSDSGSCGNLIIDFYWGME 181

Query: 185 LYPRIGKHFDIKVFTNCRFGMMSWAVLAVTYCIKQYEKNGTIADSMLVNTALMLAYVTKF 244
           LYPRIGK FDIKVFTNCRFGMMSWAVLAVTYCIKQYE NG ++DSMLVNT LML YVTKF
Sbjct: 182 LYPRIGKSFDIKVFTNCRFGMMSWAVLAVTYCIKQYEINGKVSDSMLVNTILMLVYVTKF 241

Query: 245 FWWEAGYWNTMDIAHDRAGFYICWGCLVWVPSVYTSPGMYLVNHPVHLGTQLAVYILVAG 304
           FWWEAGYWNTMDIAHDRAGFYICWGCLVWVPSVYTSPGMYLVNHPV LGTQLA+YILVAG
Sbjct: 242 FWWEAGYWNTMDIAHDRAGFYICWGCLVWVPSVYTSPGMYLVNHPVELGTQLAIYILVAG 301

Query: 305 MLCIYINYDCDRQRQEFRRKNGKCLVWGKAPSKIVASYTTTSGETKTSLLLTSGWWGLAR 364
           +LCIYINYDCDRQRQEFRR NGKCLVWG+APSKIVASYTTTSGETKTSLLLTSGWWGLAR
Sbjct: 302 ILCIYINYDCDRQRQEFRRTNGKCLVWGRAPSKIVASYTTTSGETKTSLLLTSGWWGLAR 361

Query: 365 HFHYVPEILAAFFWTVPALFSHFLPYFYVIFLTILLVDRAKRDDDRCRSKYGKYWKLYCE 424
           HFHYVPEIL+AFFWTVPALF +FL YFYVIFLT+LL DRAKRDDDRCRSKYGKYWKLYCE
Sbjct: 362 HFHYVPEILSAFFWTVPALFDNFLAYFYVIFLTLLLFDRAKRDDDRCRSKYGKYWKLYCE 421

Query: 425 KVPYRIVPGI 435
           KV YRI+PGI
Sbjct: 422 KVKYRIIPGI 431

BLAST of Carg13881 vs. TAIR 10
Match: AT1G50430.2 (Ergosterol biosynthesis ERG4/ERG24 family )

HSP 1 Score: 781.6 bits (2017), Expect = 3.4e-226
Identity = 363/430 (84.42%), Postives = 391/430 (90.93%), Query Frame = 0

Query: 5   SNTVHSPLVTYASMLSLLTLCPPFVIFLWYTMVHADGSVSQTMAYLYHNGLRGFVDIWPP 64
           + TVHSP+VTYASMLSLL  CPPFVI LWYTMVH DGSV+QT  + + NG++G ++IWP 
Sbjct: 2   AETVHSPIVTYASMLSLLAFCPPFVILLWYTMVHQDGSVTQTFGFFWENGVQGLINIWPR 61

Query: 65  PSATAFKLIGSYAAFEAALQLLLPGKTVYGPISPTGNQPVYKANGVAAYVVTLVTYIALW 124
           P+  A+K+I  Y AFEA LQLLLPGK V GPISP GN+PVYKANG+AAY VTL TY+ L 
Sbjct: 62  PTLIAWKIIFCYGAFEAILQLLLPGKRVEGPISPAGNRPVYKANGLAAYFVTLATYLGL- 121

Query: 125 WFGIFNPTVVYDHLGEIFSALIIGSFIFCIVLYIKGCVAPSSSDSGSSGNIIIDFYWGME 184
           WFGIFNP +VYDHLGEIFSALI GSFIFC++LYIKG VAPSSSDSGS GN+IIDFYWGME
Sbjct: 122 WFGIFNPAIVYDHLGEIFSALIFGSFIFCVLLYIKGHVAPSSSDSGSCGNLIIDFYWGME 181

Query: 185 LYPRIGKHFDIKVFTNCRFGMMSWAVLAVTYCIKQYEKNGTIADSMLVNTALMLAYVTKF 244
           LYPRIGK FDIKVFTNCRFGMMSWAVLAVTYCIKQYE NG ++DSMLVNT LML YVTKF
Sbjct: 182 LYPRIGKSFDIKVFTNCRFGMMSWAVLAVTYCIKQYEINGKVSDSMLVNTILMLVYVTKF 241

Query: 245 FWWEAGYWNTMDIAHDRAGFYICWGCLVWVPSVYTSPGMYLVNHPVHLGTQLAVYILVAG 304
           FWWEAGYWNTMDIAHDRAGFYICWGCLVWVPSVYTSPGMYLVNHPV LGTQLA+YILVAG
Sbjct: 242 FWWEAGYWNTMDIAHDRAGFYICWGCLVWVPSVYTSPGMYLVNHPVELGTQLAIYILVAG 301

Query: 305 MLCIYINYDCDRQRQEFRRKNGKCLVWGKAPSKIVASYTTTSGETKTSLLLTSGWWGLAR 364
           +LCIYINYDCDRQRQEFRR NGKCLVWG+APSKIVASYTTTSGETKTSLLLTSGWWGLAR
Sbjct: 302 ILCIYINYDCDRQRQEFRRTNGKCLVWGRAPSKIVASYTTTSGETKTSLLLTSGWWGLAR 361

Query: 365 HFHYVPEILAAFFWTVPALFSHFLPYFYVIFLTILLVDRAKRDDDRCRSKYGKYWKLYCE 424
           HFHYVPEIL+AFFWTVPALF +FL YFYVIFLT+LL DRAKRDDDRCRSKYGKYWKLYCE
Sbjct: 362 HFHYVPEILSAFFWTVPALFDNFLAYFYVIFLTLLLFDRAKRDDDRCRSKYGKYWKLYCE 421

Query: 425 KVPYRIVPGI 435
           KV YRI+PGI
Sbjct: 422 KVKYRIIPGI 430

BLAST of Carg13881 vs. TAIR 10
Match: AT3G52940.1 (Ergosterol biosynthesis ERG4/ERG24 family )

HSP 1 Score: 184.9 bits (468), Expect = 1.4e-46
Identity = 120/378 (31.75%), Postives = 199/378 (52.65%), Query Frame = 0

Query: 65  PSATAFKLIGSYAAFEAALQLLLPGKTVYGPISPTGNQPVYKANGVAAYVVTLVTYIALW 124
           PS  +  ++  Y  + A    +LPGK + G +   G+Q  Y+ NG+ A ++ +       
Sbjct: 12  PSLQSVYVLVFYFVYLAVAGEILPGKVIRGVLLSDGSQLRYRCNGLLALILLVAILGICA 71

Query: 125 WFGIFNPTVVYDHLGEIFSALIIGSFIFCIVLYIKGCVAPSSSDSGS------SGNIIID 184
             GI +P VV D   E+ SA  I   +  + LY+ G    SSS+ GS      SGN++ D
Sbjct: 72  KLGIVSPLVVADRGLELLSATFIFCVLVTLALYVTG---RSSSNKGSSLKPHVSGNLVHD 131

Query: 185 FYWGMELYPRIGKHFDIKVFTNCRFGMMSWAVLAVTYCIKQYEKNGTIADSMLVNTALML 244
           +++G++L P+     D+K F   R GMM W ++ ++   K  + +G+++ SM++      
Sbjct: 132 WWFGIQLNPQF-MSIDLKFFF-VRAGMMGWLLINLSILAKSVQ-DGSLSQSMILYQIFCA 191

Query: 245 AYVTKFFWWEAGYWNTMDIAHDRAGFYICWGCLVWVPSVYTSPGMYLVNHPVHLGTQLAV 304
            Y+  +F  E    +T DI  +R GF + +G L+W+P  ++  G +L+++ V    +L V
Sbjct: 192 LYILDYFVHEEYMTSTWDIIAERLGFMLVFGDLLWIPFTFSIQGWWLLHNKV----ELTV 251

Query: 305 YILVAGMLCIYINY----DCDRQRQEFRRKNGKCLVWGKAPSKIVASYTTTSGETKTSLL 364
             +V   L   I Y      ++Q+  F +KN K  +WGK P           G+     L
Sbjct: 252 PAIVVNCLVFLIGYMVFRGANKQKHIF-KKNPKTPIWGKPP-------VVVGGK-----L 311

Query: 365 LTSGWWGLARHFHYVPEILAAFFWTVPALFSHFLPYFYVIFLTILLVDRAKRDDDRCRSK 424
           L SG+WG+ARH +Y+ +++ A  +++P   S  +PYFY I+L ILL+ R +RD+ RC  K
Sbjct: 312 LVSGYWGIARHCNYLGDLMLALSFSLPCGISSPVPYFYPIYLLILLIWRERRDEVRCAEK 366

Query: 425 YGKYWKLYCEKVPYRIVP 433
           Y + W  Y   VP+RI+P
Sbjct: 372 YKEIWAEYLRLVPWRILP 366

BLAST of Carg13881 vs. TAIR 10
Match: AT3G52940.2 (Ergosterol biosynthesis ERG4/ERG24 family )

HSP 1 Score: 99.0 bits (245), Expect = 1.0e-20
Identity = 65/221 (29.41%), Postives = 114/221 (51.58%), Query Frame = 0

Query: 65  PSATAFKLIGSYAAFEAALQLLLPGKTVYGPISPTGNQPVYKANGVAAYVVTLVTYIALW 124
           PS  +  ++  Y  + A    +LPGK + G +   G+Q  Y+ NG+ A ++ +       
Sbjct: 12  PSLQSVYVLVFYFVYLAVAGEILPGKVIRGVLLSDGSQLRYRCNGLLALILLVAILGICA 71

Query: 125 WFGIFNPTVVYDHLGEIFSALIIGSFIFCIVLYIKGCVAPSSSDSGS------SGNIIID 184
             GI +P VV D   E+ SA  I   +  + LY+ G    SSS+ GS      SGN++ D
Sbjct: 72  KLGIVSPLVVADRGLELLSATFIFCVLVTLALYVTG---RSSSNKGSSLKPHVSGNLVHD 131

Query: 185 FYWGMELYPRIGKHFDIKVFTNCRFGMMSWAVLAVTYCIKQYEKNGTIADSMLVNTALML 244
           +++G++L P+     D+K F   R GMM W ++ ++   K  + +G+++ SM++      
Sbjct: 132 WWFGIQLNPQF-MSIDLKFFF-VRAGMMGWLLINLSILAKSVQ-DGSLSQSMILYQIFCA 191

Query: 245 AYVTKFFWWEAGYWNTMDIAHDRAGFYICWGCLVWVPSVYT 280
            Y+  +F  E    +T DI  +R GF + +G L+W+P  ++
Sbjct: 192 LYILDYFVHEEYMTSTWDIIAERLGFMLVFGDLLWIPFTFS 226

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
KAG7025947.18.5e-264100.007-dehydrocholesterol reductase [Cucurbita argyrosperma subsp. argyrosperma][more]
KAG6593601.11.9e-26399.777-dehydrocholesterol reductase, partial [Cucurbita argyrosperma subsp. sororia][more]
XP_022964233.11.0e-26198.857-dehydrocholesterol reductase [Cucurbita moschata][more]
XP_023513993.12.8e-25998.397-dehydrocholesterol reductase [Cucurbita pepo subsp. pepo][more]
XP_022999960.17.0e-25897.707-dehydrocholesterol reductase [Cucurbita maxima][more]
Match NameE-valueIdentityDescription
Q9LDU61.3e-22784.657-dehydrocholesterol reductase OS=Arabidopsis thaliana OX=3702 GN=DWF5 PE=1 SV=1[more]
O884553.9e-7837.537-dehydrocholesterol reductase OS=Mus musculus OX=10090 GN=Dhcr7 PE=1 SV=1[more]
Q9Z2Z81.5e-7736.887-dehydrocholesterol reductase OS=Rattus norvegicus OX=10116 GN=Dhcr7 PE=1 SV=1[more]
Q7ZXH14.3e-7736.327-dehydrocholesterol reductase OS=Xenopus laevis OX=8355 GN=dhcr7 PE=2 SV=1[more]
Q5E9J53.7e-7635.787-dehydrocholesterol reductase OS=Bos taurus OX=9913 GN=DHCR7 PE=2 SV=1[more]
Match NameE-valueIdentityDescription
A0A6J1HK805.0e-26298.857-dehydrocholesterol reductase OS=Cucurbita moschata OX=3662 GN=LOC111464317 PE=... [more]
A0A6J1KH143.4e-25897.707-dehydrocholesterol reductase OS=Cucurbita maxima OX=3661 GN=LOC111494288 PE=3 ... [more]
A0A6J1D3V82.8e-24491.717-dehydrocholesterol reductase OS=Momordica charantia OX=3673 GN=LOC111016744 PE... [more]
A0A0A0KC989.2e-24089.63Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_7G447780 PE=3 SV=1[more]
A0A5A7UXH51.6e-23990.557-dehydrocholesterol reductase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_s... [more]
Match NameE-valueIdentityDescription
AT1G50430.19.5e-22984.65Ergosterol biosynthesis ERG4/ERG24 family [more]
AT1G50430.23.4e-22684.42Ergosterol biosynthesis ERG4/ERG24 family [more]
AT3G52940.11.4e-4631.75Ergosterol biosynthesis ERG4/ERG24 family [more]
AT3G52940.21.0e-2029.41Ergosterol biosynthesis ERG4/ERG24 family [more]
InterPro
Analysis Name: InterPro Annotations of Silver-seed gourd (SMH-JMG-627) v2
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR001171Ergosterol biosynthesis ERG4/ERG24PFAMPF01222ERG4_ERG24coord: 45..434
e-value: 6.2E-87
score: 292.1
NoneNo IPR availableGENE3D1.20.120.1630coord: 225..434
e-value: 1.2E-16
score: 63.0
NoneNo IPR availablePANTHERPTHR21257DELTA(14)-STEROL REDUCTASEcoord: 6..434
NoneNo IPR availablePANTHERPTHR21257:SF537-DEHYDROCHOLESTEROL REDUCTASEcoord: 6..434
IPR018083Sterol reductase, conserved sitePROSITEPS01018STEROL_REDUCT_2coord: 399..422
IPR018083Sterol reductase, conserved sitePROSITEPS01017STEROL_REDUCT_1coord: 173..188

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Carg13881-RACarg13881-RAmRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0016132 brassinosteroid biosynthetic process
biological_process GO:0016126 sterol biosynthetic process
cellular_component GO:0030176 integral component of endoplasmic reticulum membrane
cellular_component GO:0016020 membrane
molecular_function GO:0016628 oxidoreductase activity, acting on the CH-CH group of donors, NAD or NADP as acceptor
molecular_function GO:0009918 sterol delta7 reductase activity