Homology
BLAST of Carg13881 vs. NCBI nr
Match:
KAG7025947.1 (7-dehydrocholesterol reductase [Cucurbita argyrosperma subsp. argyrosperma])
HSP 1 Score: 919.8 bits (2376), Expect = 8.5e-264
Identity = 435/435 (100.00%), Postives = 435/435 (100.00%), Query Frame = 0
Query: 1 MAEGSNTVHSPLVTYASMLSLLTLCPPFVIFLWYTMVHADGSVSQTMAYLYHNGLRGFVD 60
MAEGSNTVHSPLVTYASMLSLLTLCPPFVIFLWYTMVHADGSVSQTMAYLYHNGLRGFVD
Sbjct: 1 MAEGSNTVHSPLVTYASMLSLLTLCPPFVIFLWYTMVHADGSVSQTMAYLYHNGLRGFVD 60
Query: 61 IWPPPSATAFKLIGSYAAFEAALQLLLPGKTVYGPISPTGNQPVYKANGVAAYVVTLVTY 120
IWPPPSATAFKLIGSYAAFEAALQLLLPGKTVYGPISPTGNQPVYKANGVAAYVVTLVTY
Sbjct: 61 IWPPPSATAFKLIGSYAAFEAALQLLLPGKTVYGPISPTGNQPVYKANGVAAYVVTLVTY 120
Query: 121 IALWWFGIFNPTVVYDHLGEIFSALIIGSFIFCIVLYIKGCVAPSSSDSGSSGNIIIDFY 180
IALWWFGIFNPTVVYDHLGEIFSALIIGSFIFCIVLYIKGCVAPSSSDSGSSGNIIIDFY
Sbjct: 121 IALWWFGIFNPTVVYDHLGEIFSALIIGSFIFCIVLYIKGCVAPSSSDSGSSGNIIIDFY 180
Query: 181 WGMELYPRIGKHFDIKVFTNCRFGMMSWAVLAVTYCIKQYEKNGTIADSMLVNTALMLAY 240
WGMELYPRIGKHFDIKVFTNCRFGMMSWAVLAVTYCIKQYEKNGTIADSMLVNTALMLAY
Sbjct: 181 WGMELYPRIGKHFDIKVFTNCRFGMMSWAVLAVTYCIKQYEKNGTIADSMLVNTALMLAY 240
Query: 241 VTKFFWWEAGYWNTMDIAHDRAGFYICWGCLVWVPSVYTSPGMYLVNHPVHLGTQLAVYI 300
VTKFFWWEAGYWNTMDIAHDRAGFYICWGCLVWVPSVYTSPGMYLVNHPVHLGTQLAVYI
Sbjct: 241 VTKFFWWEAGYWNTMDIAHDRAGFYICWGCLVWVPSVYTSPGMYLVNHPVHLGTQLAVYI 300
Query: 301 LVAGMLCIYINYDCDRQRQEFRRKNGKCLVWGKAPSKIVASYTTTSGETKTSLLLTSGWW 360
LVAGMLCIYINYDCDRQRQEFRRKNGKCLVWGKAPSKIVASYTTTSGETKTSLLLTSGWW
Sbjct: 301 LVAGMLCIYINYDCDRQRQEFRRKNGKCLVWGKAPSKIVASYTTTSGETKTSLLLTSGWW 360
Query: 361 GLARHFHYVPEILAAFFWTVPALFSHFLPYFYVIFLTILLVDRAKRDDDRCRSKYGKYWK 420
GLARHFHYVPEILAAFFWTVPALFSHFLPYFYVIFLTILLVDRAKRDDDRCRSKYGKYWK
Sbjct: 361 GLARHFHYVPEILAAFFWTVPALFSHFLPYFYVIFLTILLVDRAKRDDDRCRSKYGKYWK 420
Query: 421 LYCEKVPYRIVPGIC 436
LYCEKVPYRIVPGIC
Sbjct: 421 LYCEKVPYRIVPGIC 435
BLAST of Carg13881 vs. NCBI nr
Match:
KAG6593601.1 (7-dehydrocholesterol reductase, partial [Cucurbita argyrosperma subsp. sororia])
HSP 1 Score: 918.7 bits (2373), Expect = 1.9e-263
Identity = 434/435 (99.77%), Postives = 435/435 (100.00%), Query Frame = 0
Query: 1 MAEGSNTVHSPLVTYASMLSLLTLCPPFVIFLWYTMVHADGSVSQTMAYLYHNGLRGFVD 60
MAEGSNTVHSPLVTYASMLSLLTLCPPFVIFLWYTMVHADGSVSQTMAYLYHNGLRGFVD
Sbjct: 1 MAEGSNTVHSPLVTYASMLSLLTLCPPFVIFLWYTMVHADGSVSQTMAYLYHNGLRGFVD 60
Query: 61 IWPPPSATAFKLIGSYAAFEAALQLLLPGKTVYGPISPTGNQPVYKANGVAAYVVTLVTY 120
IWPPPSATAFKLIGSYAAFEAALQLLLPGKTVYGPISPTGNQPVYKANGVAAYVVTLVTY
Sbjct: 61 IWPPPSATAFKLIGSYAAFEAALQLLLPGKTVYGPISPTGNQPVYKANGVAAYVVTLVTY 120
Query: 121 IALWWFGIFNPTVVYDHLGEIFSALIIGSFIFCIVLYIKGCVAPSSSDSGSSGNIIIDFY 180
IALWWFGIFNPTVVYDHLGEIFSALIIGSFIFCIVLYIKGCVAPSSSDSGSSGNIIIDFY
Sbjct: 121 IALWWFGIFNPTVVYDHLGEIFSALIIGSFIFCIVLYIKGCVAPSSSDSGSSGNIIIDFY 180
Query: 181 WGMELYPRIGKHFDIKVFTNCRFGMMSWAVLAVTYCIKQYEKNGTIADSMLVNTALMLAY 240
WGMELYPRIGKHFDIKVFTNCRFGMMSWAVLAVTYCIKQYEKNGTIADSMLVNTALMLAY
Sbjct: 181 WGMELYPRIGKHFDIKVFTNCRFGMMSWAVLAVTYCIKQYEKNGTIADSMLVNTALMLAY 240
Query: 241 VTKFFWWEAGYWNTMDIAHDRAGFYICWGCLVWVPSVYTSPGMYLVNHPVHLGTQLAVYI 300
VTKFFWWEAGYWNTMDIAHDRAGFYICWGCLVWVPSVYTSPGMYLVNHPVHLGTQLAVYI
Sbjct: 241 VTKFFWWEAGYWNTMDIAHDRAGFYICWGCLVWVPSVYTSPGMYLVNHPVHLGTQLAVYI 300
Query: 301 LVAGMLCIYINYDCDRQRQEFRRKNGKCLVWGKAPSKIVASYTTTSGETKTSLLLTSGWW 360
LVAGMLCIYINYDCDRQRQEFRRKNGKCLVWGKAPSKIVASYTTTSGETKTSLLLTSGWW
Sbjct: 301 LVAGMLCIYINYDCDRQRQEFRRKNGKCLVWGKAPSKIVASYTTTSGETKTSLLLTSGWW 360
Query: 361 GLARHFHYVPEILAAFFWTVPALFSHFLPYFYVIFLTILLVDRAKRDDDRCRSKYGKYWK 420
GLARHFHYVPEILAAFFWTVPALF+HFLPYFYVIFLTILLVDRAKRDDDRCRSKYGKYWK
Sbjct: 361 GLARHFHYVPEILAAFFWTVPALFNHFLPYFYVIFLTILLVDRAKRDDDRCRSKYGKYWK 420
Query: 421 LYCEKVPYRIVPGIC 436
LYCEKVPYRIVPGIC
Sbjct: 421 LYCEKVPYRIVPGIC 435
BLAST of Carg13881 vs. NCBI nr
Match:
XP_022964233.1 (7-dehydrocholesterol reductase [Cucurbita moschata])
HSP 1 Score: 912.9 bits (2358), Expect = 1.0e-261
Identity = 430/435 (98.85%), Postives = 434/435 (99.77%), Query Frame = 0
Query: 1 MAEGSNTVHSPLVTYASMLSLLTLCPPFVIFLWYTMVHADGSVSQTMAYLYHNGLRGFVD 60
MAEGSNTVHSPLVTYASMLSLLTLCPPFVIFLWYTMVHADGSVSQTMAYLYHNGLRG +D
Sbjct: 1 MAEGSNTVHSPLVTYASMLSLLTLCPPFVIFLWYTMVHADGSVSQTMAYLYHNGLRGLID 60
Query: 61 IWPPPSATAFKLIGSYAAFEAALQLLLPGKTVYGPISPTGNQPVYKANGVAAYVVTLVTY 120
IWPPPSATAFKLIGSYAAFEAALQLLLPGKTVYGPISPTGNQPVYKANGVAAYVVTLVTY
Sbjct: 61 IWPPPSATAFKLIGSYAAFEAALQLLLPGKTVYGPISPTGNQPVYKANGVAAYVVTLVTY 120
Query: 121 IALWWFGIFNPTVVYDHLGEIFSALIIGSFIFCIVLYIKGCVAPSSSDSGSSGNIIIDFY 180
IALWWFGIFNPTVVYDHLGEIFSALIIGSFIFCIVLYIKGCVAPSSSDSGSSGNIIIDFY
Sbjct: 121 IALWWFGIFNPTVVYDHLGEIFSALIIGSFIFCIVLYIKGCVAPSSSDSGSSGNIIIDFY 180
Query: 181 WGMELYPRIGKHFDIKVFTNCRFGMMSWAVLAVTYCIKQYEKNGTIADSMLVNTALMLAY 240
WGMELYPRIGKHFDIKVFTNCRFGMMSWAVLAVTYCIKQYEKNGTIADSMLVNTALMLAY
Sbjct: 181 WGMELYPRIGKHFDIKVFTNCRFGMMSWAVLAVTYCIKQYEKNGTIADSMLVNTALMLAY 240
Query: 241 VTKFFWWEAGYWNTMDIAHDRAGFYICWGCLVWVPSVYTSPGMYLVNHPVHLGTQLAVYI 300
VTKFFWWEAGYWNTMDIAHDRAGFYICWGCLVWVPSVYTSPGMYLVNHPV+LGTQLAVYI
Sbjct: 241 VTKFFWWEAGYWNTMDIAHDRAGFYICWGCLVWVPSVYTSPGMYLVNHPVNLGTQLAVYI 300
Query: 301 LVAGMLCIYINYDCDRQRQEFRRKNGKCLVWGKAPSKIVASYTTTSGETKTSLLLTSGWW 360
LVAGMLCIYINYDCDRQRQEFRRKNGKCLVWGKAPSKIVASYTTTSGETKTSLLLTSGWW
Sbjct: 301 LVAGMLCIYINYDCDRQRQEFRRKNGKCLVWGKAPSKIVASYTTTSGETKTSLLLTSGWW 360
Query: 361 GLARHFHYVPEILAAFFWTVPALFSHFLPYFYVIFLTILLVDRAKRDDDRCRSKYGKYWK 420
GLARHFHYVPEILAAFFWTVPALF+HFLPYFYVIFLTILLVDRAKRDDDRCRSKYGKYWK
Sbjct: 361 GLARHFHYVPEILAAFFWTVPALFNHFLPYFYVIFLTILLVDRAKRDDDRCRSKYGKYWK 420
Query: 421 LYCEKVPYRIVPGIC 436
LYCEKVPYRI+PGIC
Sbjct: 421 LYCEKVPYRIIPGIC 435
BLAST of Carg13881 vs. NCBI nr
Match:
XP_023513993.1 (7-dehydrocholesterol reductase [Cucurbita pepo subsp. pepo])
HSP 1 Score: 904.8 bits (2337), Expect = 2.8e-259
Identity = 428/435 (98.39%), Postives = 432/435 (99.31%), Query Frame = 0
Query: 1 MAEGSNTVHSPLVTYASMLSLLTLCPPFVIFLWYTMVHADGSVSQTMAYLYHNGLRGFVD 60
MAEGSNTVHSPLVTYASMLSLLTLCPPFVIFLWYTMVHADGSVSQTMAYLY NGLRGF+D
Sbjct: 1 MAEGSNTVHSPLVTYASMLSLLTLCPPFVIFLWYTMVHADGSVSQTMAYLYRNGLRGFID 60
Query: 61 IWPPPSATAFKLIGSYAAFEAALQLLLPGKTVYGPISPTGNQPVYKANGVAAYVVTLVTY 120
IWPPPSATAFKLIGSYAAFEAALQLLLPGKTVYGPISPTGNQPVYKANGVAAYVVTLVTY
Sbjct: 61 IWPPPSATAFKLIGSYAAFEAALQLLLPGKTVYGPISPTGNQPVYKANGVAAYVVTLVTY 120
Query: 121 IALWWFGIFNPTVVYDHLGEIFSALIIGSFIFCIVLYIKGCVAPSSSDSGSSGNIIIDFY 180
IALW FGIFNPTVVYDHLGEIFSALIIGSFIFCIVLYIKGCVAPSSSDSGSSGNIIIDFY
Sbjct: 121 IALWRFGIFNPTVVYDHLGEIFSALIIGSFIFCIVLYIKGCVAPSSSDSGSSGNIIIDFY 180
Query: 181 WGMELYPRIGKHFDIKVFTNCRFGMMSWAVLAVTYCIKQYEKNGTIADSMLVNTALMLAY 240
WGMELYPRIGKHFDIKVFTNCRFGMMSWAVLAVTYCIKQYEKNGTIADSMLVNTALMLAY
Sbjct: 181 WGMELYPRIGKHFDIKVFTNCRFGMMSWAVLAVTYCIKQYEKNGTIADSMLVNTALMLAY 240
Query: 241 VTKFFWWEAGYWNTMDIAHDRAGFYICWGCLVWVPSVYTSPGMYLVNHPVHLGTQLAVYI 300
VTKFFWWEAGYWNTMDIAHDRAGFYICWGCLVWVPSVYTSPGMYLVNHPV+LGTQLAVYI
Sbjct: 241 VTKFFWWEAGYWNTMDIAHDRAGFYICWGCLVWVPSVYTSPGMYLVNHPVNLGTQLAVYI 300
Query: 301 LVAGMLCIYINYDCDRQRQEFRRKNGKCLVWGKAPSKIVASYTTTSGETKTSLLLTSGWW 360
LVAGMLCIYINYDCDRQRQEFRRKNGKCLVWGKAPSKIVASYTT SGETKTSLLLTSGWW
Sbjct: 301 LVAGMLCIYINYDCDRQRQEFRRKNGKCLVWGKAPSKIVASYTTASGETKTSLLLTSGWW 360
Query: 361 GLARHFHYVPEILAAFFWTVPALFSHFLPYFYVIFLTILLVDRAKRDDDRCRSKYGKYWK 420
GLARHFHYVPEILAAFFWTVPALF+HFLPYFYVIFLTILLVDRAKRDDDRCRSKYGKYWK
Sbjct: 361 GLARHFHYVPEILAAFFWTVPALFNHFLPYFYVIFLTILLVDRAKRDDDRCRSKYGKYWK 420
Query: 421 LYCEKVPYRIVPGIC 436
LYCEKVPYRI+PGIC
Sbjct: 421 LYCEKVPYRIIPGIC 435
BLAST of Carg13881 vs. NCBI nr
Match:
XP_022999960.1 (7-dehydrocholesterol reductase [Cucurbita maxima])
HSP 1 Score: 900.2 bits (2325), Expect = 7.0e-258
Identity = 425/435 (97.70%), Postives = 431/435 (99.08%), Query Frame = 0
Query: 1 MAEGSNTVHSPLVTYASMLSLLTLCPPFVIFLWYTMVHADGSVSQTMAYLYHNGLRGFVD 60
MAEGSNTVHSPLVTYASMLSLLTLCPPFVIFLWYTMVHADGSVSQTMAYLYHNGLRGF+D
Sbjct: 1 MAEGSNTVHSPLVTYASMLSLLTLCPPFVIFLWYTMVHADGSVSQTMAYLYHNGLRGFID 60
Query: 61 IWPPPSATAFKLIGSYAAFEAALQLLLPGKTVYGPISPTGNQPVYKANGVAAYVVTLVTY 120
IWPPPSATAFKLIGSYAAFEAALQLLLPGKTVYGPISPTGNQPVYKANGVAAYVVTLVTY
Sbjct: 61 IWPPPSATAFKLIGSYAAFEAALQLLLPGKTVYGPISPTGNQPVYKANGVAAYVVTLVTY 120
Query: 121 IALWWFGIFNPTVVYDHLGEIFSALIIGSFIFCIVLYIKGCVAPSSSDSGSSGNIIIDFY 180
IALW FGIFNPTVVYDHLGEIFS LIIGSFIFCIVLYIKGCVAPSSSDSGSSG+IIIDFY
Sbjct: 121 IALWRFGIFNPTVVYDHLGEIFSTLIIGSFIFCIVLYIKGCVAPSSSDSGSSGDIIIDFY 180
Query: 181 WGMELYPRIGKHFDIKVFTNCRFGMMSWAVLAVTYCIKQYEKNGTIADSMLVNTALMLAY 240
WGMELYPRIGKHFDIKVFTNCRFGMMSWAVLAVTYCI QYEKNGTIADSMLVNTALMLAY
Sbjct: 181 WGMELYPRIGKHFDIKVFTNCRFGMMSWAVLAVTYCINQYEKNGTIADSMLVNTALMLAY 240
Query: 241 VTKFFWWEAGYWNTMDIAHDRAGFYICWGCLVWVPSVYTSPGMYLVNHPVHLGTQLAVYI 300
VTKFFWWEAGYWNTMDIAHDRAGFYICWGCLVWVPSVYTSPGMYLVNHPV+LG QLAVYI
Sbjct: 241 VTKFFWWEAGYWNTMDIAHDRAGFYICWGCLVWVPSVYTSPGMYLVNHPVNLGIQLAVYI 300
Query: 301 LVAGMLCIYINYDCDRQRQEFRRKNGKCLVWGKAPSKIVASYTTTSGETKTSLLLTSGWW 360
LVAGMLCIYINYDCDRQRQEFRRKNGKCLVWGKAPSKIVASYTTTSGETKTSLLLTSGWW
Sbjct: 301 LVAGMLCIYINYDCDRQRQEFRRKNGKCLVWGKAPSKIVASYTTTSGETKTSLLLTSGWW 360
Query: 361 GLARHFHYVPEILAAFFWTVPALFSHFLPYFYVIFLTILLVDRAKRDDDRCRSKYGKYWK 420
GLARHFHYVPEILAAFFWTVPALF+HFLPYFYVIFLTILLVDRAKRDD+RCRSKYGKYWK
Sbjct: 361 GLARHFHYVPEILAAFFWTVPALFNHFLPYFYVIFLTILLVDRAKRDDNRCRSKYGKYWK 420
Query: 421 LYCEKVPYRIVPGIC 436
LYCEKVPYRI+PGIC
Sbjct: 421 LYCEKVPYRIIPGIC 435
BLAST of Carg13881 vs. ExPASy Swiss-Prot
Match:
Q9LDU6 (7-dehydrocholesterol reductase OS=Arabidopsis thaliana OX=3702 GN=DWF5 PE=1 SV=1)
HSP 1 Score: 790.0 bits (2039), Expect = 1.3e-227
Identity = 364/430 (84.65%), Postives = 392/430 (91.16%), Query Frame = 0
Query: 5 SNTVHSPLVTYASMLSLLTLCPPFVIFLWYTMVHADGSVSQTMAYLYHNGLRGFVDIWPP 64
+ TVHSP+VTYASMLSLL CPPFVI LWYTMVH DGSV+QT + + NG++G ++IWP
Sbjct: 2 AETVHSPIVTYASMLSLLAFCPPFVILLWYTMVHQDGSVTQTFGFFWENGVQGLINIWPR 61
Query: 65 PSATAFKLIGSYAAFEAALQLLLPGKTVYGPISPTGNQPVYKANGVAAYVVTLVTYIALW 124
P+ A+K+I Y AFEA LQLLLPGK V GPISP GN+PVYKANG+AAY VTL TY+ LW
Sbjct: 62 PTLIAWKIIFCYGAFEAILQLLLPGKRVEGPISPAGNRPVYKANGLAAYFVTLATYLGLW 121
Query: 125 WFGIFNPTVVYDHLGEIFSALIIGSFIFCIVLYIKGCVAPSSSDSGSSGNIIIDFYWGME 184
WFGIFNP +VYDHLGEIFSALI GSFIFC++LYIKG VAPSSSDSGS GN+IIDFYWGME
Sbjct: 122 WFGIFNPAIVYDHLGEIFSALIFGSFIFCVLLYIKGHVAPSSSDSGSCGNLIIDFYWGME 181
Query: 185 LYPRIGKHFDIKVFTNCRFGMMSWAVLAVTYCIKQYEKNGTIADSMLVNTALMLAYVTKF 244
LYPRIGK FDIKVFTNCRFGMMSWAVLAVTYCIKQYE NG ++DSMLVNT LML YVTKF
Sbjct: 182 LYPRIGKSFDIKVFTNCRFGMMSWAVLAVTYCIKQYEINGKVSDSMLVNTILMLVYVTKF 241
Query: 245 FWWEAGYWNTMDIAHDRAGFYICWGCLVWVPSVYTSPGMYLVNHPVHLGTQLAVYILVAG 304
FWWEAGYWNTMDIAHDRAGFYICWGCLVWVPSVYTSPGMYLVNHPV LGTQLA+YILVAG
Sbjct: 242 FWWEAGYWNTMDIAHDRAGFYICWGCLVWVPSVYTSPGMYLVNHPVELGTQLAIYILVAG 301
Query: 305 MLCIYINYDCDRQRQEFRRKNGKCLVWGKAPSKIVASYTTTSGETKTSLLLTSGWWGLAR 364
+LCIYINYDCDRQRQEFRR NGKCLVWG+APSKIVASYTTTSGETKTSLLLTSGWWGLAR
Sbjct: 302 ILCIYINYDCDRQRQEFRRTNGKCLVWGRAPSKIVASYTTTSGETKTSLLLTSGWWGLAR 361
Query: 365 HFHYVPEILAAFFWTVPALFSHFLPYFYVIFLTILLVDRAKRDDDRCRSKYGKYWKLYCE 424
HFHYVPEIL+AFFWTVPALF +FL YFYVIFLT+LL DRAKRDDDRCRSKYGKYWKLYCE
Sbjct: 362 HFHYVPEILSAFFWTVPALFDNFLAYFYVIFLTLLLFDRAKRDDDRCRSKYGKYWKLYCE 421
Query: 425 KVPYRIVPGI 435
KV YRI+PGI
Sbjct: 422 KVKYRIIPGI 431
BLAST of Carg13881 vs. ExPASy Swiss-Prot
Match:
O88455 (7-dehydrocholesterol reductase OS=Mus musculus OX=10090 GN=Dhcr7 PE=1 SV=1)
HSP 1 Score: 293.5 bits (750), Expect = 3.9e-78
Identity = 164/437 (37.53%), Postives = 252/437 (57.67%), Query Frame = 0
Query: 16 ASMLSLLTLCPPFVIFLWYTMVHADGSVSQTMAYL-YHNGLRGFVDIW---PPPSATAFK 75
AS++ LL L PF+++ ++ M S S T L G DIW PP +A A +
Sbjct: 37 ASIIFLL-LFAPFIVY-YFIMACDQYSCSLTAPALDIATGHASLADIWAKTPPVTAKAAQ 96
Query: 76 LIGSYAAFEAALQLLLP-----------GKTVYGPISPTGNQPVYKANGVAAYVVT-LVT 135
L + +F+ L LP G G I+P G Y+ NG+ A+++T ++
Sbjct: 97 LYALWVSFQVLLYSWLPDFCHRFLPGYVGGVQEGAITPAGVVNKYEVNGLQAWLITHILW 156
Query: 136 YIALWWFGIFNPTVVYDH-LGEIFSALIIGSFIFCIVLYIKGCVAPSSS-DSGSSGNIII 195
++ + F+PT+++D+ + ++ A I+G + + IKG + P+S+ D +GN
Sbjct: 157 FVNAYLLSWFSPTIIFDNWIPLLWCANILGYAVSTFAM-IKGYLFPTSAEDCKFTGNFFY 216
Query: 196 DFYWGMELYPRIGKHFDIKVFTNCRFGMMSWAVLAVTYCIKQYEKNGTIADSMLVNTALM 255
++ G+E PRIGK FD K+F N R G+++W ++ +++ KQ E G + +SM++ L
Sbjct: 217 NYMMGIEFNPRIGKWFDFKLFFNGRPGIVAWTLINLSFAAKQQELYGHVTNSMILVNVLQ 276
Query: 256 LAYVTKFFWWEAGYWNTMDIAHDRAGFYICWGCLVWVPSVYTSPGMYLVNHPVHLGTQLA 315
YV FFW E Y T+DI HD G+Y+ WG VW+P +YT G+YLV HPV L T A
Sbjct: 277 AIYVLDFFWNETWYLKTIDICHDHFGWYLGWGDCVWLPYLYTLQGLYLVYHPVQLSTPNA 336
Query: 316 VYILVAGMLCIYINYDCDRQRQEFRRKNGKCLVWGKAPSKIVASYTTTSGETKTSLLLTS 375
+ IL+ G++ YI + Q+ FRR +G+CL+WGK P I SYT+ G S LL S
Sbjct: 337 LGILLLGLVGYYIFRMTNHQKDLFRRTDGRCLIWGKKPKAIECSYTSADGLKHHSKLLVS 396
Query: 376 GWWGLARHFHYVPEILAAFFWTVPALFSHFLPYFYVIFLTILLVDRAKRDDDRCRSKYGK 435
G+WG+ARHF+Y +++ + + + H LPYFY+I++TILL R RD+ RC +KYG+
Sbjct: 397 GFWGVARHFNYTGDLMGSLAYCLACGGGHLLPYFYIIYMTILLTHRCLRDEHRCANKYGR 456
BLAST of Carg13881 vs. ExPASy Swiss-Prot
Match:
Q9Z2Z8 (7-dehydrocholesterol reductase OS=Rattus norvegicus OX=10116 GN=Dhcr7 PE=1 SV=1)
HSP 1 Score: 291.6 bits (745), Expect = 1.5e-77
Identity = 163/442 (36.88%), Postives = 252/442 (57.01%), Query Frame = 0
Query: 13 VTYASMLSL--LTLCPPFVIFLWYTMVHADGSVSQTMAYL-YHNGLRGFVDIW---PPPS 72
V + S++S+ L L PF+++ ++ M S S T L G DIW PP +
Sbjct: 31 VDWFSLVSVIFLLLFAPFIVY-YFIMACDQYSCSLTAPILDVATGRASLADIWAKTPPVT 90
Query: 73 ATAFKLIGSYAAFEAALQLLLP-----------GKTVYGPISPTGNQPVYKANGVAAYVV 132
A A +L + +F+ L LP G G I+P G Y+ NG+ A+++
Sbjct: 91 AKAAQLYALWVSFQVLLYSWLPDFCHRFLPGYVGGVQEGAITPAGIVNKYEVNGLQAWLI 150
Query: 133 T-LVTYIALWWFGIFNPTVVYDH-LGEIFSALIIGSFIFCIVLYIKGCVAPSSS-DSGSS 192
T + ++ + F+PT+++D+ + ++ A I+G + + IKG + P+S+ D +
Sbjct: 151 THFLWFVNAYLLSWFSPTIIFDNWIPLLWCANILGYAVSTFAM-IKGYLFPTSAEDCKFT 210
Query: 193 GNIIIDFYWGMELYPRIGKHFDIKVFTNCRFGMMSWAVLAVTYCIKQYEKNGTIADSMLV 252
GN ++ G+E PRIGK FD K+F N R G+++W ++ +++ KQ E G + +SM++
Sbjct: 211 GNFFYNYMMGIEFNPRIGKWFDFKLFFNGRPGIVAWTLINLSFAAKQQELYGHVTNSMIL 270
Query: 253 NTALMLAYVTKFFWWEAGYWNTMDIAHDRAGFYICWGCLVWVPSVYTSPGMYLVNHPVHL 312
L YV FFW E Y T+DI HD G+Y+ WG VW+P +YT G+YLV HPV L
Sbjct: 271 VNVLQAIYVLDFFWNETWYLKTIDICHDHFGWYLGWGDCVWLPYLYTLQGLYLVYHPVQL 330
Query: 313 GTQLAVYILVAGMLCIYINYDCDRQRQEFRRKNGKCLVWGKAPSKIVASYTTTSGETKTS 372
T A+ +L+ G++ YI + Q+ FRR +G CL+WGK P I SYT+ G S
Sbjct: 331 STPNALGVLLLGLVGYYIFRMTNHQKDLFRRTDGHCLIWGKKPKAIECSYTSADGLKHRS 390
Query: 373 LLLTSGWWGLARHFHYVPEILAAFFWTVPALFSHFLPYFYVIFLTILLVDRAKRDDDRCR 432
LL SG+WG+ARHF+Y +++ + + + H LPYFY+I++TILL R RD+ RC
Sbjct: 391 KLLVSGFWGVARHFNYTGDLMGSLAYCLACGGGHLLPYFYIIYMTILLTHRCLRDEHRCA 450
Query: 433 SKYGKYWKLYCEKVPYRIVPGI 435
+KYG+ W+ Y VPYR++PGI
Sbjct: 451 NKYGRDWERYVAAVPYRLLPGI 470
BLAST of Carg13881 vs. ExPASy Swiss-Prot
Match:
Q7ZXH1 (7-dehydrocholesterol reductase OS=Xenopus laevis OX=8355 GN=dhcr7 PE=2 SV=1)
HSP 1 Score: 290.0 bits (741), Expect = 4.3e-77
Identity = 158/435 (36.32%), Postives = 242/435 (55.63%), Query Frame = 0
Query: 16 ASMLSLLTLCPPFVIFLWYTMVHADGSVSQTMAYLYHNGLRGFVDIW---PPPSATAFKL 75
AS++ LL P V + + +++ + LY +G DIW P + TA K+
Sbjct: 39 ASVIFLLAFAPLIVYYFVMSCDQYQCALTAPVLDLY-SGKARLSDIWDKTPALTWTAVKI 98
Query: 76 IGSYAAFEAALQLLLP-----------GKTVYGPISPTGNQPVYKANGVAAYVVT-LVTY 135
++ +F+ L + LP G G +P G Y+ NG+ A+ +T L+ +
Sbjct: 99 YLAWVSFQVFLYMFLPDILHKFVPGYEGGVQEGARTPAGLINKYQVNGLQAWTITHLLWF 158
Query: 136 IALWWFGIFNPTVVYDH-LGEIFSALIIGSFIFCIVLYIKGCVAPSSSDSGSSGNIIIDF 195
+ F F+PT+V D+ + ++ A ++G + L +++D +GN D+
Sbjct: 159 ANAYHFHWFSPTIVIDNWIPLLWCANLLGYSVATFALVKANFFPTNANDCKFTGNFFYDY 218
Query: 196 YWGMELYPRIGKHFDIKVFTNCRFGMMSWAVLAVTYCIKQYEKNGTIADSMLVNTALMLA 255
G+E PRIGK FD K+F N R G+++W ++ ++Y KQ E G + +SM++ L
Sbjct: 219 MMGIEFNPRIGKWFDFKLFFNGRPGIVAWTLINLSYAAKQQELYGQVTNSMILVNVLQAI 278
Query: 256 YVTKFFWWEAGYWNTMDIAHDRAGFYICWGCLVWVPSVYTSPGMYLVNHPVHLGTQLAVY 315
YV FFW E+ Y T+DI HD G+Y+ WG VW+P +YT G+YLV +PV L T AV
Sbjct: 279 YVVDFFWNESWYLKTIDICHDHFGWYLGWGDCVWLPYLYTLQGLYLVYNPVELSTTAAVA 338
Query: 316 ILVAGMLCIYINYDCDRQRQEFRRKNGKCLVWGKAPSKIVASYTTTSGETKTSLLLTSGW 375
+L+ G++ YI + Q+ FRR NG C +WGK P I Y + G+ S L+ SG+
Sbjct: 339 VLLLGLIGYYIFRMTNHQKDLFRRTNGNCKIWGKKPKSIECFYVSADGKRHYSKLMISGF 398
Query: 376 WGLARHFHYVPEILAAFFWTVPALFSHFLPYFYVIFLTILLVDRAKRDDDRCRSKYGKYW 435
WG+ARH +Y +++ + + + F H LPYFY I++TILLV R RD+ RC SKYGK W
Sbjct: 399 WGVARHLNYTGDLMGSLAYCLACGFDHLLPYFYFIYMTILLVHRCIRDEHRCSSKYGKDW 458
BLAST of Carg13881 vs. ExPASy Swiss-Prot
Match:
Q5E9J5 (7-dehydrocholesterol reductase OS=Bos taurus OX=9913 GN=DHCR7 PE=2 SV=1)
HSP 1 Score: 287.0 bits (733), Expect = 3.7e-76
Identity = 156/436 (35.78%), Postives = 246/436 (56.42%), Query Frame = 0
Query: 16 ASMLSLLTLCPPFVIFLWYTMVHADGSVSQTMAYLYHNGLRGFVDIW---PPPSATAFKL 75
AS++ LL L PF+++ + G G DIW PP +A A ++
Sbjct: 41 ASVIFLL-LFAPFIVYYFIMACDQYGCSLTVPVADLATGRARLADIWARTPPVTAKAAQI 100
Query: 76 IGSYAAFEAALQLLLP-----------GKTVYGPISPTGNQPVYKANGVAAYVVT-LVTY 135
++ + L +LLP G G ++P G Y+ NG+ A+++T L+ +
Sbjct: 101 YTAWVTLQVLLYMLLPDFCHKFLPGYVGGVQEGAVTPAGAVNKYEINGLQAWLLTHLLWF 160
Query: 136 IALWWFGIFNPTVVYDH-LGEIFSALIIGSFIFCIVLYIKGCVAPSSS-DSGSSGNIIID 195
G F+PT+++D+ + ++ A I+G + + +KG + P+ + + +GN +
Sbjct: 161 ANAHLLGWFSPTIIFDNWIPLLWCANILGYTVSTFAM-VKGYLFPTDARECKFTGNFFYN 220
Query: 196 FYWGMELYPRIGKHFDIKVFTNCRFGMMSWAVLAVTYCIKQYEKNGTIADSMLVNTALML 255
+ G+E PRIGK FD K+F N R G+++W ++ +++ KQ E G + +SM++ L
Sbjct: 221 YMMGVEFNPRIGKWFDFKLFFNGRPGIVAWTLINLSFAAKQQELYGHVTNSMVLVNILQA 280
Query: 256 AYVTKFFWWEAGYWNTMDIAHDRAGFYICWGCLVWVPSVYTSPGMYLVNHPVHLGTQLAV 315
YV FFW E Y T+DI HD G+Y+ WG VW+P +YT G+YLV HPV L T A+
Sbjct: 281 IYVLDFFWNETWYLKTIDICHDHFGWYLGWGDCVWLPYLYTLQGLYLVYHPVQLPTYYAL 340
Query: 316 YILVAGMLCIYINYDCDRQRQEFRRKNGKCLVWGKAPSKIVASYTTTSGETKTSLLLTSG 375
+L+ G+L YI + Q+ FRR +G+CL+WG+ P I SYT+ G+ S LL SG
Sbjct: 341 GVLLLGLLGYYIFRMTNHQKDLFRRTDGRCLIWGRKPKAIECSYTSADGQRHHSKLLVSG 400
Query: 376 WWGLARHFHYVPEILAAFFWTVPALFSHFLPYFYVIFLTILLVDRAKRDDDRCRSKYGKY 435
+WG+ARHF+Y +++ + + + H LPYFY+IF+ ILL R RD+ RC +KYG+
Sbjct: 401 FWGVARHFNYTGDLMGSLAYCLACGGGHLLPYFYIIFMAILLTHRCLRDEHRCANKYGRD 460
BLAST of Carg13881 vs. ExPASy TrEMBL
Match:
A0A6J1HK80 (7-dehydrocholesterol reductase OS=Cucurbita moschata OX=3662 GN=LOC111464317 PE=3 SV=1)
HSP 1 Score: 912.9 bits (2358), Expect = 5.0e-262
Identity = 430/435 (98.85%), Postives = 434/435 (99.77%), Query Frame = 0
Query: 1 MAEGSNTVHSPLVTYASMLSLLTLCPPFVIFLWYTMVHADGSVSQTMAYLYHNGLRGFVD 60
MAEGSNTVHSPLVTYASMLSLLTLCPPFVIFLWYTMVHADGSVSQTMAYLYHNGLRG +D
Sbjct: 1 MAEGSNTVHSPLVTYASMLSLLTLCPPFVIFLWYTMVHADGSVSQTMAYLYHNGLRGLID 60
Query: 61 IWPPPSATAFKLIGSYAAFEAALQLLLPGKTVYGPISPTGNQPVYKANGVAAYVVTLVTY 120
IWPPPSATAFKLIGSYAAFEAALQLLLPGKTVYGPISPTGNQPVYKANGVAAYVVTLVTY
Sbjct: 61 IWPPPSATAFKLIGSYAAFEAALQLLLPGKTVYGPISPTGNQPVYKANGVAAYVVTLVTY 120
Query: 121 IALWWFGIFNPTVVYDHLGEIFSALIIGSFIFCIVLYIKGCVAPSSSDSGSSGNIIIDFY 180
IALWWFGIFNPTVVYDHLGEIFSALIIGSFIFCIVLYIKGCVAPSSSDSGSSGNIIIDFY
Sbjct: 121 IALWWFGIFNPTVVYDHLGEIFSALIIGSFIFCIVLYIKGCVAPSSSDSGSSGNIIIDFY 180
Query: 181 WGMELYPRIGKHFDIKVFTNCRFGMMSWAVLAVTYCIKQYEKNGTIADSMLVNTALMLAY 240
WGMELYPRIGKHFDIKVFTNCRFGMMSWAVLAVTYCIKQYEKNGTIADSMLVNTALMLAY
Sbjct: 181 WGMELYPRIGKHFDIKVFTNCRFGMMSWAVLAVTYCIKQYEKNGTIADSMLVNTALMLAY 240
Query: 241 VTKFFWWEAGYWNTMDIAHDRAGFYICWGCLVWVPSVYTSPGMYLVNHPVHLGTQLAVYI 300
VTKFFWWEAGYWNTMDIAHDRAGFYICWGCLVWVPSVYTSPGMYLVNHPV+LGTQLAVYI
Sbjct: 241 VTKFFWWEAGYWNTMDIAHDRAGFYICWGCLVWVPSVYTSPGMYLVNHPVNLGTQLAVYI 300
Query: 301 LVAGMLCIYINYDCDRQRQEFRRKNGKCLVWGKAPSKIVASYTTTSGETKTSLLLTSGWW 360
LVAGMLCIYINYDCDRQRQEFRRKNGKCLVWGKAPSKIVASYTTTSGETKTSLLLTSGWW
Sbjct: 301 LVAGMLCIYINYDCDRQRQEFRRKNGKCLVWGKAPSKIVASYTTTSGETKTSLLLTSGWW 360
Query: 361 GLARHFHYVPEILAAFFWTVPALFSHFLPYFYVIFLTILLVDRAKRDDDRCRSKYGKYWK 420
GLARHFHYVPEILAAFFWTVPALF+HFLPYFYVIFLTILLVDRAKRDDDRCRSKYGKYWK
Sbjct: 361 GLARHFHYVPEILAAFFWTVPALFNHFLPYFYVIFLTILLVDRAKRDDDRCRSKYGKYWK 420
Query: 421 LYCEKVPYRIVPGIC 436
LYCEKVPYRI+PGIC
Sbjct: 421 LYCEKVPYRIIPGIC 435
BLAST of Carg13881 vs. ExPASy TrEMBL
Match:
A0A6J1KH14 (7-dehydrocholesterol reductase OS=Cucurbita maxima OX=3661 GN=LOC111494288 PE=3 SV=1)
HSP 1 Score: 900.2 bits (2325), Expect = 3.4e-258
Identity = 425/435 (97.70%), Postives = 431/435 (99.08%), Query Frame = 0
Query: 1 MAEGSNTVHSPLVTYASMLSLLTLCPPFVIFLWYTMVHADGSVSQTMAYLYHNGLRGFVD 60
MAEGSNTVHSPLVTYASMLSLLTLCPPFVIFLWYTMVHADGSVSQTMAYLYHNGLRGF+D
Sbjct: 1 MAEGSNTVHSPLVTYASMLSLLTLCPPFVIFLWYTMVHADGSVSQTMAYLYHNGLRGFID 60
Query: 61 IWPPPSATAFKLIGSYAAFEAALQLLLPGKTVYGPISPTGNQPVYKANGVAAYVVTLVTY 120
IWPPPSATAFKLIGSYAAFEAALQLLLPGKTVYGPISPTGNQPVYKANGVAAYVVTLVTY
Sbjct: 61 IWPPPSATAFKLIGSYAAFEAALQLLLPGKTVYGPISPTGNQPVYKANGVAAYVVTLVTY 120
Query: 121 IALWWFGIFNPTVVYDHLGEIFSALIIGSFIFCIVLYIKGCVAPSSSDSGSSGNIIIDFY 180
IALW FGIFNPTVVYDHLGEIFS LIIGSFIFCIVLYIKGCVAPSSSDSGSSG+IIIDFY
Sbjct: 121 IALWRFGIFNPTVVYDHLGEIFSTLIIGSFIFCIVLYIKGCVAPSSSDSGSSGDIIIDFY 180
Query: 181 WGMELYPRIGKHFDIKVFTNCRFGMMSWAVLAVTYCIKQYEKNGTIADSMLVNTALMLAY 240
WGMELYPRIGKHFDIKVFTNCRFGMMSWAVLAVTYCI QYEKNGTIADSMLVNTALMLAY
Sbjct: 181 WGMELYPRIGKHFDIKVFTNCRFGMMSWAVLAVTYCINQYEKNGTIADSMLVNTALMLAY 240
Query: 241 VTKFFWWEAGYWNTMDIAHDRAGFYICWGCLVWVPSVYTSPGMYLVNHPVHLGTQLAVYI 300
VTKFFWWEAGYWNTMDIAHDRAGFYICWGCLVWVPSVYTSPGMYLVNHPV+LG QLAVYI
Sbjct: 241 VTKFFWWEAGYWNTMDIAHDRAGFYICWGCLVWVPSVYTSPGMYLVNHPVNLGIQLAVYI 300
Query: 301 LVAGMLCIYINYDCDRQRQEFRRKNGKCLVWGKAPSKIVASYTTTSGETKTSLLLTSGWW 360
LVAGMLCIYINYDCDRQRQEFRRKNGKCLVWGKAPSKIVASYTTTSGETKTSLLLTSGWW
Sbjct: 301 LVAGMLCIYINYDCDRQRQEFRRKNGKCLVWGKAPSKIVASYTTTSGETKTSLLLTSGWW 360
Query: 361 GLARHFHYVPEILAAFFWTVPALFSHFLPYFYVIFLTILLVDRAKRDDDRCRSKYGKYWK 420
GLARHFHYVPEILAAFFWTVPALF+HFLPYFYVIFLTILLVDRAKRDD+RCRSKYGKYWK
Sbjct: 361 GLARHFHYVPEILAAFFWTVPALFNHFLPYFYVIFLTILLVDRAKRDDNRCRSKYGKYWK 420
Query: 421 LYCEKVPYRIVPGIC 436
LYCEKVPYRI+PGIC
Sbjct: 421 LYCEKVPYRIIPGIC 435
BLAST of Carg13881 vs. ExPASy TrEMBL
Match:
A0A6J1D3V8 (7-dehydrocholesterol reductase OS=Momordica charantia OX=3673 GN=LOC111016744 PE=3 SV=1)
HSP 1 Score: 854.0 bits (2205), Expect = 2.8e-244
Identity = 398/434 (91.71%), Postives = 416/434 (95.85%), Query Frame = 0
Query: 1 MAEGSNTVHSPLVTYASMLSLLTLCPPFVIFLWYTMVHADGSVSQTMAYLYHNGLRGFVD 60
MAEGS TVHSP++TY SMLSLL +CPPFVIFLWYTMVHADGSVSQT YLYHNG+RGF++
Sbjct: 1 MAEGSTTVHSPILTYVSMLSLLIICPPFVIFLWYTMVHADGSVSQTWNYLYHNGVRGFMN 60
Query: 61 IWPPPSATAFKLIGSYAAFEAALQLLLPGKTVYGPISPTGNQPVYKANGVAAYVVTLVTY 120
IWPPPSATA K+IG YAAFEAALQLLLPG V GP+SPTGNQPVYKANGVAAY+VTLVTY
Sbjct: 61 IWPPPSATACKIIGCYAAFEAALQLLLPGNRVQGPVSPTGNQPVYKANGVAAYIVTLVTY 120
Query: 121 IALWWFGIFNPTVVYDHLGEIFSALIIGSFIFCIVLYIKGCVAPSSSDSGSSGNIIIDFY 180
IALWWFGIFNPT+VYDHLGEIFSALI GS IFCI LYIKG VAPSSSDSGSSGNIIIDFY
Sbjct: 121 IALWWFGIFNPTIVYDHLGEIFSALIFGSLIFCIFLYIKGHVAPSSSDSGSSGNIIIDFY 180
Query: 181 WGMELYPRIGKHFDIKVFTNCRFGMMSWAVLAVTYCIKQYEKNGTIADSMLVNTALMLAY 240
WGMELYPRIGK+FDIKVFTNCRFGMMSWAVLAVTYCIKQYEKNGTIADSMLVNTALML Y
Sbjct: 181 WGMELYPRIGKNFDIKVFTNCRFGMMSWAVLAVTYCIKQYEKNGTIADSMLVNTALMLVY 240
Query: 241 VTKFFWWEAGYWNTMDIAHDRAGFYICWGCLVWVPSVYTSPGMYLVNHPVHLGTQLAVYI 300
VTKFFWWE+GYWNTMDIAHDRAGFYICWGCLVWVPSVYTSPGMYLVNHPV+LGTQLAVYI
Sbjct: 241 VTKFFWWESGYWNTMDIAHDRAGFYICWGCLVWVPSVYTSPGMYLVNHPVNLGTQLAVYI 300
Query: 301 LVAGMLCIYINYDCDRQRQEFRRKNGKCLVWGKAPSKIVASYTTTSGETKTSLLLTSGWW 360
LVAG+LCIYINYDCDRQRQEFRRKNGKCL+W KAPSKIVASYTTTSGETKTSLLLTSGWW
Sbjct: 301 LVAGLLCIYINYDCDRQRQEFRRKNGKCLIWRKAPSKIVASYTTTSGETKTSLLLTSGWW 360
Query: 361 GLARHFHYVPEILAAFFWTVPALFSHFLPYFYVIFLTILLVDRAKRDDDRCRSKYGKYWK 420
GLARHFHY PEILAAFFWTVPALF+HFLPYFYVIFLTILL+DRAKRDDDRCRSKYGKYWK
Sbjct: 361 GLARHFHYAPEILAAFFWTVPALFNHFLPYFYVIFLTILLLDRAKRDDDRCRSKYGKYWK 420
Query: 421 LYCEKVPYRIVPGI 435
LYCEKV YRI+PGI
Sbjct: 421 LYCEKVAYRIIPGI 434
BLAST of Carg13881 vs. ExPASy TrEMBL
Match:
A0A0A0KC98 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_7G447780 PE=3 SV=1)
HSP 1 Score: 839.0 bits (2166), Expect = 9.2e-240
Identity = 389/434 (89.63%), Postives = 412/434 (94.93%), Query Frame = 0
Query: 1 MAEGSNTVHSPLVTYASMLSLLTLCPPFVIFLWYTMVHADGSVSQTMAYLYHNGLRGFVD 60
MAEGSNTVHSP +TY SMLSLLTLCPPFVI LWYTMVHADGSVSQT YLY NG GF++
Sbjct: 1 MAEGSNTVHSPFLTYVSMLSLLTLCPPFVILLWYTMVHADGSVSQTWNYLYQNGFEGFIN 60
Query: 61 IWPPPSATAFKLIGSYAAFEAALQLLLPGKTVYGPISPTGNQPVYKANGVAAYVVTLVTY 120
IWP PSATA K+IG YAAFEAALQLLLPGK V GP+SPTGNQPVYKANGVAAY+VTL+TY
Sbjct: 61 IWPSPSATACKIIGCYAAFEAALQLLLPGKRVLGPVSPTGNQPVYKANGVAAYIVTLLTY 120
Query: 121 IALWWFGIFNPTVVYDHLGEIFSALIIGSFIFCIVLYIKGCVAPSSSDSGSSGNIIIDFY 180
+ALW FGIF+P +VYDHLGEI+S LI GSFIFCI+LYIKG VAPSSSDSGSSGN+I+DFY
Sbjct: 121 VALWRFGIFDPAIVYDHLGEIYSTLIFGSFIFCILLYIKGHVAPSSSDSGSSGNVILDFY 180
Query: 181 WGMELYPRIGKHFDIKVFTNCRFGMMSWAVLAVTYCIKQYEKNGTIADSMLVNTALMLAY 240
WGMELYPRIGKHFDIKVFTNCRFGMMSWAVLAVTYCIKQYEKNGT+ADS+LVNTALML Y
Sbjct: 181 WGMELYPRIGKHFDIKVFTNCRFGMMSWAVLAVTYCIKQYEKNGTLADSLLVNTALMLVY 240
Query: 241 VTKFFWWEAGYWNTMDIAHDRAGFYICWGCLVWVPSVYTSPGMYLVNHPVHLGTQLAVYI 300
VTKFFWWEAGYWNTMDIAHDRAGFYICWGCLVWVPSVYTSPGMYLVNHPV+LGTQLAV I
Sbjct: 241 VTKFFWWEAGYWNTMDIAHDRAGFYICWGCLVWVPSVYTSPGMYLVNHPVNLGTQLAVSI 300
Query: 301 LVAGMLCIYINYDCDRQRQEFRRKNGKCLVWGKAPSKIVASYTTTSGETKTSLLLTSGWW 360
LVAG+LCIYINYDCDRQRQEFRRKNGKCLVWGKAPSKIVA+YTTT+GET+TSLLLTSGWW
Sbjct: 301 LVAGILCIYINYDCDRQRQEFRRKNGKCLVWGKAPSKIVATYTTTTGETRTSLLLTSGWW 360
Query: 361 GLARHFHYVPEILAAFFWTVPALFSHFLPYFYVIFLTILLVDRAKRDDDRCRSKYGKYWK 420
GLARHFHYVPEILAAFFWTVPALF HFLPYFYVIFLTILL DRAKRDDDRCRSKYGKYWK
Sbjct: 361 GLARHFHYVPEILAAFFWTVPALFHHFLPYFYVIFLTILLFDRAKRDDDRCRSKYGKYWK 420
Query: 421 LYCEKVPYRIVPGI 435
LYCEKVPY+I+PGI
Sbjct: 421 LYCEKVPYKIIPGI 434
BLAST of Carg13881 vs. ExPASy TrEMBL
Match:
A0A5A7UXH5 (7-dehydrocholesterol reductase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold347G001860 PE=3 SV=1)
HSP 1 Score: 838.2 bits (2164), Expect = 1.6e-239
Identity = 393/434 (90.55%), Postives = 410/434 (94.47%), Query Frame = 0
Query: 1 MAEGSNTVHSPLVTYASMLSLLTLCPPFVIFLWYTMVHADGSVSQTMAYLYHNGLRGFVD 60
MAEGSNTVHSP +TY SMLSLLTLCPPFVI LWYTMVHADGSVSQT YLY NG GF++
Sbjct: 1 MAEGSNTVHSPFLTYVSMLSLLTLCPPFVILLWYTMVHADGSVSQTWNYLYQNGFEGFIN 60
Query: 61 IWPPPSATAFKLIGSYAAFEAALQLLLPGKTVYGPISPTGNQPVYKANGVAAYVVTLVTY 120
IWP PSATA K+IG YAAFEAALQLLLPGK V GP+SPTGNQPVYKANGVAAY+VTLVTY
Sbjct: 61 IWPSPSATACKIIGCYAAFEAALQLLLPGKRVLGPVSPTGNQPVYKANGVAAYIVTLVTY 120
Query: 121 IALWWFGIFNPTVVYDHLGEIFSALIIGSFIFCIVLYIKGCVAPSSSDSGSSGNIIIDFY 180
+ALW FGIF+P +VYDHLGEIFS LI GSFIFCI+LYIKG VAPSSSDSGSSGNII+DFY
Sbjct: 121 VALWRFGIFDPAIVYDHLGEIFSTLIFGSFIFCILLYIKGHVAPSSSDSGSSGNIILDFY 180
Query: 181 WGMELYPRIGKHFDIKVFTNCRFGMMSWAVLAVTYCIKQYEKNGTIADSMLVNTALMLAY 240
WGMELYPRIGKHFDIKVFTNCRFGMMSWAVLAVTYCIKQYEKNG +ADSMLVNTALML Y
Sbjct: 181 WGMELYPRIGKHFDIKVFTNCRFGMMSWAVLAVTYCIKQYEKNGILADSMLVNTALMLVY 240
Query: 241 VTKFFWWEAGYWNTMDIAHDRAGFYICWGCLVWVPSVYTSPGMYLVNHPVHLGTQLAVYI 300
VTKFFWWEAGYWNTMDIAHDRAGFYICWGCLVWVPSVYTSPGMYLVNHPV+LGTQLAV I
Sbjct: 241 VTKFFWWEAGYWNTMDIAHDRAGFYICWGCLVWVPSVYTSPGMYLVNHPVNLGTQLAVSI 300
Query: 301 LVAGMLCIYINYDCDRQRQEFRRKNGKCLVWGKAPSKIVASYTTTSGETKTSLLLTSGWW 360
LVAG+LCIYINYDCDRQRQEFRRKNGK LVWGKAPSKIVASYTTTSGET+TSLLLTSGWW
Sbjct: 301 LVAGILCIYINYDCDRQRQEFRRKNGKRLVWGKAPSKIVASYTTTSGETRTSLLLTSGWW 360
Query: 361 GLARHFHYVPEILAAFFWTVPALFSHFLPYFYVIFLTILLVDRAKRDDDRCRSKYGKYWK 420
GLARHFHYVPEILAAFFWTVPALF HFLPYFYVIFLTILL DRAKRDDDRCRSKYGKYWK
Sbjct: 361 GLARHFHYVPEILAAFFWTVPALFHHFLPYFYVIFLTILLFDRAKRDDDRCRSKYGKYWK 420
Query: 421 LYCEKVPYRIVPGI 435
LYCEKVPY+I+PGI
Sbjct: 421 LYCEKVPYKIIPGI 434
BLAST of Carg13881 vs. TAIR 10
Match:
AT1G50430.1 (Ergosterol biosynthesis ERG4/ERG24 family )
HSP 1 Score: 790.0 bits (2039), Expect = 9.5e-229
Identity = 364/430 (84.65%), Postives = 392/430 (91.16%), Query Frame = 0
Query: 5 SNTVHSPLVTYASMLSLLTLCPPFVIFLWYTMVHADGSVSQTMAYLYHNGLRGFVDIWPP 64
+ TVHSP+VTYASMLSLL CPPFVI LWYTMVH DGSV+QT + + NG++G ++IWP
Sbjct: 2 AETVHSPIVTYASMLSLLAFCPPFVILLWYTMVHQDGSVTQTFGFFWENGVQGLINIWPR 61
Query: 65 PSATAFKLIGSYAAFEAALQLLLPGKTVYGPISPTGNQPVYKANGVAAYVVTLVTYIALW 124
P+ A+K+I Y AFEA LQLLLPGK V GPISP GN+PVYKANG+AAY VTL TY+ LW
Sbjct: 62 PTLIAWKIIFCYGAFEAILQLLLPGKRVEGPISPAGNRPVYKANGLAAYFVTLATYLGLW 121
Query: 125 WFGIFNPTVVYDHLGEIFSALIIGSFIFCIVLYIKGCVAPSSSDSGSSGNIIIDFYWGME 184
WFGIFNP +VYDHLGEIFSALI GSFIFC++LYIKG VAPSSSDSGS GN+IIDFYWGME
Sbjct: 122 WFGIFNPAIVYDHLGEIFSALIFGSFIFCVLLYIKGHVAPSSSDSGSCGNLIIDFYWGME 181
Query: 185 LYPRIGKHFDIKVFTNCRFGMMSWAVLAVTYCIKQYEKNGTIADSMLVNTALMLAYVTKF 244
LYPRIGK FDIKVFTNCRFGMMSWAVLAVTYCIKQYE NG ++DSMLVNT LML YVTKF
Sbjct: 182 LYPRIGKSFDIKVFTNCRFGMMSWAVLAVTYCIKQYEINGKVSDSMLVNTILMLVYVTKF 241
Query: 245 FWWEAGYWNTMDIAHDRAGFYICWGCLVWVPSVYTSPGMYLVNHPVHLGTQLAVYILVAG 304
FWWEAGYWNTMDIAHDRAGFYICWGCLVWVPSVYTSPGMYLVNHPV LGTQLA+YILVAG
Sbjct: 242 FWWEAGYWNTMDIAHDRAGFYICWGCLVWVPSVYTSPGMYLVNHPVELGTQLAIYILVAG 301
Query: 305 MLCIYINYDCDRQRQEFRRKNGKCLVWGKAPSKIVASYTTTSGETKTSLLLTSGWWGLAR 364
+LCIYINYDCDRQRQEFRR NGKCLVWG+APSKIVASYTTTSGETKTSLLLTSGWWGLAR
Sbjct: 302 ILCIYINYDCDRQRQEFRRTNGKCLVWGRAPSKIVASYTTTSGETKTSLLLTSGWWGLAR 361
Query: 365 HFHYVPEILAAFFWTVPALFSHFLPYFYVIFLTILLVDRAKRDDDRCRSKYGKYWKLYCE 424
HFHYVPEIL+AFFWTVPALF +FL YFYVIFLT+LL DRAKRDDDRCRSKYGKYWKLYCE
Sbjct: 362 HFHYVPEILSAFFWTVPALFDNFLAYFYVIFLTLLLFDRAKRDDDRCRSKYGKYWKLYCE 421
Query: 425 KVPYRIVPGI 435
KV YRI+PGI
Sbjct: 422 KVKYRIIPGI 431
BLAST of Carg13881 vs. TAIR 10
Match:
AT1G50430.2 (Ergosterol biosynthesis ERG4/ERG24 family )
HSP 1 Score: 781.6 bits (2017), Expect = 3.4e-226
Identity = 363/430 (84.42%), Postives = 391/430 (90.93%), Query Frame = 0
Query: 5 SNTVHSPLVTYASMLSLLTLCPPFVIFLWYTMVHADGSVSQTMAYLYHNGLRGFVDIWPP 64
+ TVHSP+VTYASMLSLL CPPFVI LWYTMVH DGSV+QT + + NG++G ++IWP
Sbjct: 2 AETVHSPIVTYASMLSLLAFCPPFVILLWYTMVHQDGSVTQTFGFFWENGVQGLINIWPR 61
Query: 65 PSATAFKLIGSYAAFEAALQLLLPGKTVYGPISPTGNQPVYKANGVAAYVVTLVTYIALW 124
P+ A+K+I Y AFEA LQLLLPGK V GPISP GN+PVYKANG+AAY VTL TY+ L
Sbjct: 62 PTLIAWKIIFCYGAFEAILQLLLPGKRVEGPISPAGNRPVYKANGLAAYFVTLATYLGL- 121
Query: 125 WFGIFNPTVVYDHLGEIFSALIIGSFIFCIVLYIKGCVAPSSSDSGSSGNIIIDFYWGME 184
WFGIFNP +VYDHLGEIFSALI GSFIFC++LYIKG VAPSSSDSGS GN+IIDFYWGME
Sbjct: 122 WFGIFNPAIVYDHLGEIFSALIFGSFIFCVLLYIKGHVAPSSSDSGSCGNLIIDFYWGME 181
Query: 185 LYPRIGKHFDIKVFTNCRFGMMSWAVLAVTYCIKQYEKNGTIADSMLVNTALMLAYVTKF 244
LYPRIGK FDIKVFTNCRFGMMSWAVLAVTYCIKQYE NG ++DSMLVNT LML YVTKF
Sbjct: 182 LYPRIGKSFDIKVFTNCRFGMMSWAVLAVTYCIKQYEINGKVSDSMLVNTILMLVYVTKF 241
Query: 245 FWWEAGYWNTMDIAHDRAGFYICWGCLVWVPSVYTSPGMYLVNHPVHLGTQLAVYILVAG 304
FWWEAGYWNTMDIAHDRAGFYICWGCLVWVPSVYTSPGMYLVNHPV LGTQLA+YILVAG
Sbjct: 242 FWWEAGYWNTMDIAHDRAGFYICWGCLVWVPSVYTSPGMYLVNHPVELGTQLAIYILVAG 301
Query: 305 MLCIYINYDCDRQRQEFRRKNGKCLVWGKAPSKIVASYTTTSGETKTSLLLTSGWWGLAR 364
+LCIYINYDCDRQRQEFRR NGKCLVWG+APSKIVASYTTTSGETKTSLLLTSGWWGLAR
Sbjct: 302 ILCIYINYDCDRQRQEFRRTNGKCLVWGRAPSKIVASYTTTSGETKTSLLLTSGWWGLAR 361
Query: 365 HFHYVPEILAAFFWTVPALFSHFLPYFYVIFLTILLVDRAKRDDDRCRSKYGKYWKLYCE 424
HFHYVPEIL+AFFWTVPALF +FL YFYVIFLT+LL DRAKRDDDRCRSKYGKYWKLYCE
Sbjct: 362 HFHYVPEILSAFFWTVPALFDNFLAYFYVIFLTLLLFDRAKRDDDRCRSKYGKYWKLYCE 421
Query: 425 KVPYRIVPGI 435
KV YRI+PGI
Sbjct: 422 KVKYRIIPGI 430
BLAST of Carg13881 vs. TAIR 10
Match:
AT3G52940.1 (Ergosterol biosynthesis ERG4/ERG24 family )
HSP 1 Score: 184.9 bits (468), Expect = 1.4e-46
Identity = 120/378 (31.75%), Postives = 199/378 (52.65%), Query Frame = 0
Query: 65 PSATAFKLIGSYAAFEAALQLLLPGKTVYGPISPTGNQPVYKANGVAAYVVTLVTYIALW 124
PS + ++ Y + A +LPGK + G + G+Q Y+ NG+ A ++ +
Sbjct: 12 PSLQSVYVLVFYFVYLAVAGEILPGKVIRGVLLSDGSQLRYRCNGLLALILLVAILGICA 71
Query: 125 WFGIFNPTVVYDHLGEIFSALIIGSFIFCIVLYIKGCVAPSSSDSGS------SGNIIID 184
GI +P VV D E+ SA I + + LY+ G SSS+ GS SGN++ D
Sbjct: 72 KLGIVSPLVVADRGLELLSATFIFCVLVTLALYVTG---RSSSNKGSSLKPHVSGNLVHD 131
Query: 185 FYWGMELYPRIGKHFDIKVFTNCRFGMMSWAVLAVTYCIKQYEKNGTIADSMLVNTALML 244
+++G++L P+ D+K F R GMM W ++ ++ K + +G+++ SM++
Sbjct: 132 WWFGIQLNPQF-MSIDLKFFF-VRAGMMGWLLINLSILAKSVQ-DGSLSQSMILYQIFCA 191
Query: 245 AYVTKFFWWEAGYWNTMDIAHDRAGFYICWGCLVWVPSVYTSPGMYLVNHPVHLGTQLAV 304
Y+ +F E +T DI +R GF + +G L+W+P ++ G +L+++ V +L V
Sbjct: 192 LYILDYFVHEEYMTSTWDIIAERLGFMLVFGDLLWIPFTFSIQGWWLLHNKV----ELTV 251
Query: 305 YILVAGMLCIYINY----DCDRQRQEFRRKNGKCLVWGKAPSKIVASYTTTSGETKTSLL 364
+V L I Y ++Q+ F +KN K +WGK P G+ L
Sbjct: 252 PAIVVNCLVFLIGYMVFRGANKQKHIF-KKNPKTPIWGKPP-------VVVGGK-----L 311
Query: 365 LTSGWWGLARHFHYVPEILAAFFWTVPALFSHFLPYFYVIFLTILLVDRAKRDDDRCRSK 424
L SG+WG+ARH +Y+ +++ A +++P S +PYFY I+L ILL+ R +RD+ RC K
Sbjct: 312 LVSGYWGIARHCNYLGDLMLALSFSLPCGISSPVPYFYPIYLLILLIWRERRDEVRCAEK 366
Query: 425 YGKYWKLYCEKVPYRIVP 433
Y + W Y VP+RI+P
Sbjct: 372 YKEIWAEYLRLVPWRILP 366
BLAST of Carg13881 vs. TAIR 10
Match:
AT3G52940.2 (Ergosterol biosynthesis ERG4/ERG24 family )
HSP 1 Score: 99.0 bits (245), Expect = 1.0e-20
Identity = 65/221 (29.41%), Postives = 114/221 (51.58%), Query Frame = 0
Query: 65 PSATAFKLIGSYAAFEAALQLLLPGKTVYGPISPTGNQPVYKANGVAAYVVTLVTYIALW 124
PS + ++ Y + A +LPGK + G + G+Q Y+ NG+ A ++ +
Sbjct: 12 PSLQSVYVLVFYFVYLAVAGEILPGKVIRGVLLSDGSQLRYRCNGLLALILLVAILGICA 71
Query: 125 WFGIFNPTVVYDHLGEIFSALIIGSFIFCIVLYIKGCVAPSSSDSGS------SGNIIID 184
GI +P VV D E+ SA I + + LY+ G SSS+ GS SGN++ D
Sbjct: 72 KLGIVSPLVVADRGLELLSATFIFCVLVTLALYVTG---RSSSNKGSSLKPHVSGNLVHD 131
Query: 185 FYWGMELYPRIGKHFDIKVFTNCRFGMMSWAVLAVTYCIKQYEKNGTIADSMLVNTALML 244
+++G++L P+ D+K F R GMM W ++ ++ K + +G+++ SM++
Sbjct: 132 WWFGIQLNPQF-MSIDLKFFF-VRAGMMGWLLINLSILAKSVQ-DGSLSQSMILYQIFCA 191
Query: 245 AYVTKFFWWEAGYWNTMDIAHDRAGFYICWGCLVWVPSVYT 280
Y+ +F E +T DI +R GF + +G L+W+P ++
Sbjct: 192 LYILDYFVHEEYMTSTWDIIAERLGFMLVFGDLLWIPFTFS 226
The following BLAST results are available for this feature:
Match Name | E-value | Identity | Description | |
KAG7025947.1 | 8.5e-264 | 100.00 | 7-dehydrocholesterol reductase [Cucurbita argyrosperma subsp. argyrosperma] | [more] |
KAG6593601.1 | 1.9e-263 | 99.77 | 7-dehydrocholesterol reductase, partial [Cucurbita argyrosperma subsp. sororia] | [more] |
XP_022964233.1 | 1.0e-261 | 98.85 | 7-dehydrocholesterol reductase [Cucurbita moschata] | [more] |
XP_023513993.1 | 2.8e-259 | 98.39 | 7-dehydrocholesterol reductase [Cucurbita pepo subsp. pepo] | [more] |
XP_022999960.1 | 7.0e-258 | 97.70 | 7-dehydrocholesterol reductase [Cucurbita maxima] | [more] |
Match Name | E-value | Identity | Description | |
Q9LDU6 | 1.3e-227 | 84.65 | 7-dehydrocholesterol reductase OS=Arabidopsis thaliana OX=3702 GN=DWF5 PE=1 SV=1 | [more] |
O88455 | 3.9e-78 | 37.53 | 7-dehydrocholesterol reductase OS=Mus musculus OX=10090 GN=Dhcr7 PE=1 SV=1 | [more] |
Q9Z2Z8 | 1.5e-77 | 36.88 | 7-dehydrocholesterol reductase OS=Rattus norvegicus OX=10116 GN=Dhcr7 PE=1 SV=1 | [more] |
Q7ZXH1 | 4.3e-77 | 36.32 | 7-dehydrocholesterol reductase OS=Xenopus laevis OX=8355 GN=dhcr7 PE=2 SV=1 | [more] |
Q5E9J5 | 3.7e-76 | 35.78 | 7-dehydrocholesterol reductase OS=Bos taurus OX=9913 GN=DHCR7 PE=2 SV=1 | [more] |
Match Name | E-value | Identity | Description | |
A0A6J1HK80 | 5.0e-262 | 98.85 | 7-dehydrocholesterol reductase OS=Cucurbita moschata OX=3662 GN=LOC111464317 PE=... | [more] |
A0A6J1KH14 | 3.4e-258 | 97.70 | 7-dehydrocholesterol reductase OS=Cucurbita maxima OX=3661 GN=LOC111494288 PE=3 ... | [more] |
A0A6J1D3V8 | 2.8e-244 | 91.71 | 7-dehydrocholesterol reductase OS=Momordica charantia OX=3673 GN=LOC111016744 PE... | [more] |
A0A0A0KC98 | 9.2e-240 | 89.63 | Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_7G447780 PE=3 SV=1 | [more] |
A0A5A7UXH5 | 1.6e-239 | 90.55 | 7-dehydrocholesterol reductase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_s... | [more] |