Carg13878 (gene) Silver-seed gourd (SMH-JMG-627) v2

Overview


Name	Carg13878
Type	gene
Organism	Cucurbita argyrosperma subsp. argyrosperma cv. SMH-JMG-627 (Silver-seed gourd (SMH-JMG-627) v2)
Description	cytochrome b5-like
Location	Carg_Chr08: 4756415 .. 4757408 (+)
RNA-Seq Expression	Carg13878
Synteny	Carg13878

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRCDSpolypeptidethree_prime_UTR

Hold the cursor over a type above to highlight its positions in the sequence below.

TTTTCTCTTCTTCTTCTTCCTCTTCGTATATTTATCGTCCCAAATTTGGTTAGGTACGGTTCCCACAAATGGATTCAAATTCTAAACTCTTTACTTTCGATGAAGTTGCAAAGCACAACCACGAGAAAGATTGTTGGCTTATCATCTCTGGTTATGTATGTTCCTTTTTTGTTTTAATTTTTTCAAAAAATCATTAATTTCCATTAAAATAAGCTCACTGTTTTTTTTTTTTGAGTTTATGGGTATTTCTATAACATTATCAGAGATTTTGATGAAATGCAACCTTTCACTGTATAAATATTATTAAATTTCATTCTTGGTCCTTTTTTATATTTTGTTTTCTATTTTCTATTTTATTATTTTCTTTTGTGATATATTTCTAATCTCTAGAGGTTGATTTAGGTGTATGATGTAACTTCTTATTTACCGGACCACCCAGGTGGCGGTGAGCTTTTGGTATTGGCTGTGGGTAAGATGCTATTGCATTCGTTTACTATCTTTTTTTTTTTTTTAATCAAAATATATCTTTTTAATAAATAGTTTATTTCATGGGAAACGTATCGAATGTAATACAAAGATTCATATTTGAATTTGCAGAAAAAGACGCGACATTTGATTTTAAGAGTGTTGGACATAGTGAAACAGCTCAAGAGATGATGAAAAAGTATCAGATAGGTAAAATCGACCCGTTGACTCTCCCGACAGAACAAAAACATGTGGAAACTACTCCACACGAACCAACTCCGAGCCTACTTTCCTTTTCGCCTTTACATGTTCTAATTCCTCTATTGCTCTTGGTCTTGGCTTTTGCTTTTCCCTATTGAAAAATTAAGGCCTACAAATAAAACTCTATTGGGGTGGATTAAGAATTTGAAGCTTCAATTCAATATCGATGATCATCGAGTTTGTACCATCTTTTTATTCGCCATTATTAGTATCGTTTAAGGTTGGATTCATACTTTTTTCATATGAATTTGTAATATATGGATAAGAT

mRNA sequence

TTTTCTCTTCTTCTTCTTCCTCTTCGTATATTTATCGTCCCAAATTTGGTTAGGTACGGTTCCCACAAATGGATTCAAATTCTAAACTCTTTACTTTCGATGAAGTTGCAAAGCACAACCACGAGAAAGATTGTTGGCTTATCATCTCTGGTTATGTGTATGATGTAACTTCTTATTTACCGGACCACCCAGGTGGCGGTGAGCTTTTGGTATTGGCTGTGGAAAAAGACGCGACATTTGATTTTAAGAGTGTTGGACATAGTGAAACAGCTCAAGAGATGATGAAAAAGTATCAGATAGGTAAAATCGACCCGTTGACTCTCCCGACAGAACAAAAACATGTGGAAACTACTCCACACGAACCAACTCCGAGCCTACTTTCCTTTTCGCCTTTACATGTTCTAATTCCTCTATTGCTCTTGGTCTTGGCTTTTGCTTTTCCCTATTGAAAAATTAAGGCCTACAAATAAAACTCTATTGGGGTGGATTAAGAATTTGAAGCTTCAATTCAATATCGATGATCATCGAGTTTGTACCATCTTTTTATTCGCCATTATTAGTATCGTTTAAGGTTGGATTCATACTTTTTTCATATGAATTTGTAATATATGGATAAGAT

Coding sequence (CDS)

ATGGATTCAAATTCTAAACTCTTTACTTTCGATGAAGTTGCAAAGCACAACCACGAGAAAGATTGTTGGCTTATCATCTCTGGTTATGTGTATGATGTAACTTCTTATTTACCGGACCACCCAGGTGGCGGTGAGCTTTTGGTATTGGCTGTGGAAAAAGACGCGACATTTGATTTTAAGAGTGTTGGACATAGTGAAACAGCTCAAGAGATGATGAAAAAGTATCAGATAGGTAAAATCGACCCGTTGACTCTCCCGACAGAACAAAAACATGTGGAAACTACTCCACACGAACCAACTCCGAGCCTACTTTCCTTTTCGCCTTTACATGTTCTAATTCCTCTATTGCTCTTGGTCTTGGCTTTTGCTTTTCCCTATTGA

Protein sequence

MDSNSKLFTFDEVAKHNHEKDCWLIISGYVYDVTSYLPDHPGGGELLVLAVEKDATFDFKSVGHSETAQEMMKKYQIGKIDPLTLPTEQKHVETTPHEPTPSLLSFSPLHVLIPLLLLVLAFAFPY

Homology

[Close]

BLAST of Carg13878 vs. NCBI nr
Match: KAG7025950.1 (Cytochrome b5 isoform E [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 258.5 bits (659), Expect = 3.1e-65
Identity = 126/126 (100.00%), Postives = 126/126 (100.00%), Query Frame = 0


Query: 1   MDSNSKLFTFDEVAKHNHEKDCWLIISGYVYDVTSYLPDHPGGGELLVLAVEKDATFDFK 60
           MDSNSKLFTFDEVAKHNHEKDCWLIISGYVYDVTSYLPDHPGGGELLVLAVEKDATFDFK
Sbjct: 1   MDSNSKLFTFDEVAKHNHEKDCWLIISGYVYDVTSYLPDHPGGGELLVLAVEKDATFDFK 60

Query: 61  SVGHSETAQEMMKKYQIGKIDPLTLPTEQKHVETTPHEPTPSLLSFSPLHVLIPLLLLVL 120
           SVGHSETAQEMMKKYQIGKIDPLTLPTEQKHVETTPHEPTPSLLSFSPLHVLIPLLLLVL
Sbjct: 61  SVGHSETAQEMMKKYQIGKIDPLTLPTEQKHVETTPHEPTPSLLSFSPLHVLIPLLLLVL 120

Query: 121 AFAFPY 127
           AFAFPY
Sbjct: 121 AFAFPY 126

[Close]

BLAST of Carg13878 vs. NCBI nr
Match: KAG6593603.1 (Cycloartenol synthase, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 255.0 bits (650), Expect = 3.4e-64
Identity = 124/126 (98.41%), Postives = 126/126 (100.00%), Query Frame = 0


Query: 1   MDSNSKLFTFDEVAKHNHEKDCWLIISGYVYDVTSYLPDHPGGGELLVLAVEKDATFDFK 60
           MDSNSKLFTFDEVAKHNHEKDCWLIISG+VYDVTSYLPDHPGGGELLVLAVEKDATFDFK
Sbjct: 584 MDSNSKLFTFDEVAKHNHEKDCWLIISGHVYDVTSYLPDHPGGGELLVLAVEKDATFDFK 643

Query: 61  SVGHSETAQEMMKKYQIGKIDPLTLPTEQKHVETTPHEPTPSLLSFSPLHVLIPLLLLVL 120
           SVGHSETAQEMMKKYQIGKIDPLTLPTEQKHVETTPH+PTPSLLSFSPLHVLIPLLLLVL
Sbjct: 644 SVGHSETAQEMMKKYQIGKIDPLTLPTEQKHVETTPHKPTPSLLSFSPLHVLIPLLLLVL 703

Query: 121 AFAFPY 127
           AFAFPY
Sbjct: 704 AFAFPY 709

[Close]

BLAST of Carg13878 vs. NCBI nr
Match: XP_022964486.1 (cytochrome b5-like [Cucurbita moschata])

HSP 1 Score: 247.7 bits (631), Expect = 5.4e-62
Identity = 119/126 (94.44%), Postives = 125/126 (99.21%), Query Frame = 0


Query: 1   MDSNSKLFTFDEVAKHNHEKDCWLIISGYVYDVTSYLPDHPGGGELLVLAVEKDATFDFK 60
           MDSNSK++TFDEVAKHNHEKDCWLIISG+VYDVTSYLPDHPGGGELLVLAVEKDATFDFK
Sbjct: 1   MDSNSKVYTFDEVAKHNHEKDCWLIISGFVYDVTSYLPDHPGGGELLVLAVEKDATFDFK 60

Query: 61  SVGHSETAQEMMKKYQIGKIDPLTLPTEQKHVETTPHEPTPSLLSFSPLHVLIPLLLLVL 120
           SVGHSE+A+E MKKYQIGKIDPLTLPTEQKHVETTPH+PTPSLLSFSPLHVLIPLLLLVL
Sbjct: 61  SVGHSESAKERMKKYQIGKIDPLTLPTEQKHVETTPHKPTPSLLSFSPLHVLIPLLLLVL 120

Query: 121 AFAFPY 127
           AFAFPY
Sbjct: 121 AFAFPY 126

[Close]

BLAST of Carg13878 vs. NCBI nr
Match: XP_023515254.1 (cytochrome b5-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 245.4 bits (625), Expect = 2.7e-61
Identity = 119/126 (94.44%), Postives = 122/126 (96.83%), Query Frame = 0


Query: 1   MDSNSKLFTFDEVAKHNHEKDCWLIISGYVYDVTSYLPDHPGGGELLVLAVEKDATFDFK 60
           MDSNSK++TFDEVAKHNHEKDCWLIISGYVYDVTSYLPDHPGGGELLVLAVEKDATFDFK
Sbjct: 1   MDSNSKVYTFDEVAKHNHEKDCWLIISGYVYDVTSYLPDHPGGGELLVLAVEKDATFDFK 60

Query: 61  SVGHSETAQEMMKKYQIGKIDPLTLPTEQKHVETTPHEPTPSLLSFSPLHVLIPLLLLVL 120
           SVGHSETAQE MKKYQIGKIDPLTLPTEQKHVETTPH+PT SLLSFSPLHVLIPLLL VL
Sbjct: 61  SVGHSETAQERMKKYQIGKIDPLTLPTEQKHVETTPHKPTKSLLSFSPLHVLIPLLLFVL 120

Query: 121 AFAFPY 127
           A AFPY
Sbjct: 121 AIAFPY 126

[Close]

BLAST of Carg13878 vs. NCBI nr
Match: XP_023000205.1 (cytochrome b5-like [Cucurbita maxima])

HSP 1 Score: 243.0 bits (619), Expect = 1.3e-60
Identity = 117/126 (92.86%), Postives = 124/126 (98.41%), Query Frame = 0


Query: 1   MDSNSKLFTFDEVAKHNHEKDCWLIISGYVYDVTSYLPDHPGGGELLVLAVEKDATFDFK 60
           MDSNSK++TF+EVAKHNHEKDCWLIISGYVYDVTSYLPDHPGG ELLVL VEKDATFDFK
Sbjct: 1   MDSNSKIYTFNEVAKHNHEKDCWLIISGYVYDVTSYLPDHPGGSELLVLTVEKDATFDFK 60

Query: 61  SVGHSETAQEMMKKYQIGKIDPLTLPTEQKHVETTPHEPTPSLLSFSPLHVLIPLLLLVL 120
           SVGHSETA+EMMKKYQIGKIDPLTLPTEQK+VETTP++PTPSLLSFSPLHVLIPLLLLVL
Sbjct: 61  SVGHSETAREMMKKYQIGKIDPLTLPTEQKYVETTPYKPTPSLLSFSPLHVLIPLLLLVL 120

Query: 121 AFAFPY 127
           AFAFPY
Sbjct: 121 AFAFPY 126

[Close]

BLAST of Carg13878 vs. ExPASy Swiss-Prot
Match: Q42342 (Cytochrome b5 isoform E OS=Arabidopsis thaliana OX=3702 GN=CYTB5-E PE=1 SV=2)

HSP 1 Score: 119.8 bits (299), Expect = 2.2e-26
Identity = 63/127 (49.61%), Postives = 87/127 (68.50%), Query Frame = 0


Query: 1   MDSNSKLFTFDEVAKHNHEKDCWLIISGYVYDVTSYLPDHPGGGELLVLAVEKDATFDFK 60
           M S+ K+ +F+EV+KHN  KDCWLIISG VYDVT ++ DHPGG E+L+ +  KDAT DF+
Sbjct: 1   MSSDRKVLSFEEVSKHNKTKDCWLIISGKVYDVTPFMDDHPGGDEVLLSSTGKDATNDFE 60

Query: 61  SVGHSETAQEMMKKYQIGKIDPLTLPTEQKHVETTPHEP------TPSLLSFSPLHVLIP 120
            VGHS+TA++MM KY IG+ID  ++P  + +V   P +P      TP  +    L  L+P
Sbjct: 61  DVGHSDTARDMMDKYFIGEIDSSSVPATRTYV--APQQPAYNQDKTPEFI-IKILQFLVP 120

Query: 121 LLLLVLA 122
           +L+L LA
Sbjct: 121 ILILGLA 124

[Close]

BLAST of Carg13878 vs. ExPASy Swiss-Prot
Match: P49098 (Cytochrome b5 OS=Nicotiana tabacum OX=4097 PE=2 SV=1)

HSP 1 Score: 118.6 bits (296), Expect = 5.0e-26
Identity = 61/131 (46.56%), Postives = 86/131 (65.65%), Query Frame = 0


Query: 1   MDSNSKLFTFDEVAKHNHEKDCWLIISGYVYDVTSYLPDHPGGGELLVLAVEKDATFDFK 60
           M   +K+FT  EV++HN+ KDCWL+ISG VYDVT +L DHPGG E+L+ A  KDAT DF+
Sbjct: 1   MGGETKVFTLAEVSQHNNAKDCWLVISGKVYDVTKFLDDHPGGDEVLLSATGKDATDDFE 60

Query: 61  SVGHSETAQEMMKKYQIGKIDPLTLPTEQKHVETTPHEP-----TPSLLSFSPLHVLIPL 120
            VGHS +A+ M+ +Y +G ID  T+PT+ K+  T P++P       S      L  L+PL
Sbjct: 61  DVGHSSSARAMLDEYYVGDIDSATIPTKTKY--TPPNQPHYNQDKTSEFVVKLLQFLVPL 120

Query: 121 LLLVLAFAFPY 127
           ++L +AF   +
Sbjct: 121 IILGVAFGIRF 129

[Close]

BLAST of Carg13878 vs. ExPASy Swiss-Prot
Match: P49099 (Cytochrome b5, seed isoform OS=Nicotiana tabacum OX=4097 PE=2 SV=1)

HSP 1 Score: 118.2 bits (295), Expect = 6.5e-26
Identity = 61/125 (48.80%), Postives = 82/125 (65.60%), Query Frame = 0


Query: 1   MDSNSKLFTFDEVAKHNHEKDCWLIISGYVYDVTSYLPDHPGGGELLVLAVEKDATFDFK 60
           M   SK+FT  EV+ HN+ KDCWLIISG VY+VT +L DHPGGGE+L+ A  KDAT DF+
Sbjct: 1   MGGQSKVFTLAEVSNHNNAKDCWLIISGKVYNVTKFLEDHPGGGEVLLSATGKDATDDFE 60

Query: 61  SVGHSETAQEMMKKYQIGKIDPLTLPTEQKHV-ETTPHEPTPSLLSF--SPLHVLIPLLL 120
            +GHS +A+ M+ +Y +G ID  T+PT+ K+     PH        F    L  L+PL++
Sbjct: 61  DIGHSSSARAMLDEYYVGDIDSSTIPTKVKYTPPKQPHYNQDKTTEFIVKLLQFLVPLII 120

Query: 121 LVLAF 123
           L +AF
Sbjct: 121 LGVAF 125

[Close]

BLAST of Carg13878 vs. ExPASy Swiss-Prot
Match: P40934 (Cytochrome b5 OS=Brassica oleracea var. botrytis OX=3715 GN=CYB5 PE=1 SV=1)

HSP 1 Score: 118.2 bits (295), Expect = 6.5e-26
Identity = 61/125 (48.80%), Postives = 84/125 (67.20%), Query Frame = 0


Query: 1   MDSNSKLFTFDEVAKHNHEKDCWLIISGYVYDVTSYLPDHPGGGELLVLAVEKDATFDFK 60
           M S  K+  F+EV++HN  KDCWLIISG VYDVT ++ DHPGG E+L+ +  KDAT DF+
Sbjct: 1   MASEKKVLGFEEVSQHNKTKDCWLIISGKVYDVTPFMDDHPGGDEVLLSSTGKDATNDFE 60

Query: 61  SVGHSETAQEMMKKYQIGKIDPLTLPTEQKHVETTP----HEPTPSLLSFSPLHVLIPLL 120
            VGHS+TA++MM+KY IG+ID  T+P  + +V         + TP  +    L  L+P+L
Sbjct: 61  DVGHSDTARDMMEKYYIGEIDSSTVPATRTYVAPVQPAYNQDKTPEFM-IKILQFLVPIL 120

Query: 121 LLVLA 122
           +L LA
Sbjct: 121 ILGLA 124

[Close]

BLAST of Carg13878 vs. ExPASy Swiss-Prot
Match: O48845 (Cytochrome b5 isoform B OS=Arabidopsis thaliana OX=3702 GN=CYTB5-B PE=1 SV=1)

HSP 1 Score: 116.3 bits (290), Expect = 2.5e-25
Identity = 58/126 (46.03%), Postives = 84/126 (66.67%), Query Frame = 0


Query: 1   MDSNSKLFTFDEVAKHNHEKDCWLIISGYVYDVTSYLPDHPGGGELLVLAVEKDATFDFK 60
           M   +K+FT  EV++HN   DCW++I+G VY+VT +L DHPGG ++L+ +  KDAT DF+
Sbjct: 1   MGDEAKIFTLSEVSEHNQAHDCWIVINGKVYNVTKFLEDHPGGDDVLLSSTGKDATDDFE 60

Query: 61  SVGHSETAQEMMKKYQIGKIDPLTLPTEQKHVETTPHEP-----TPSLLSFSPLHVLIPL 120
            VGHSE+A+EMM++Y +G+IDP T+P + K+  T P +P       S      L  L+PL
Sbjct: 61  DVGHSESAREMMEQYYVGEIDPTTIPKKVKY--TPPKQPHYNQDKTSEFIIKLLQFLVPL 120

Query: 121 LLLVLA 122
            +L LA
Sbjct: 121 AILGLA 124

[Close]

BLAST of Carg13878 vs. ExPASy TrEMBL
Match: A0A6J1HJ10 (cytochrome b5-like OS=Cucurbita moschata OX=3662 GN=LOC111464496 PE=3 SV=1)

HSP 1 Score: 247.7 bits (631), Expect = 2.6e-62
Identity = 119/126 (94.44%), Postives = 125/126 (99.21%), Query Frame = 0


Query: 1   MDSNSKLFTFDEVAKHNHEKDCWLIISGYVYDVTSYLPDHPGGGELLVLAVEKDATFDFK 60
           MDSNSK++TFDEVAKHNHEKDCWLIISG+VYDVTSYLPDHPGGGELLVLAVEKDATFDFK
Sbjct: 1   MDSNSKVYTFDEVAKHNHEKDCWLIISGFVYDVTSYLPDHPGGGELLVLAVEKDATFDFK 60

Query: 61  SVGHSETAQEMMKKYQIGKIDPLTLPTEQKHVETTPHEPTPSLLSFSPLHVLIPLLLLVL 120
           SVGHSE+A+E MKKYQIGKIDPLTLPTEQKHVETTPH+PTPSLLSFSPLHVLIPLLLLVL
Sbjct: 61  SVGHSESAKERMKKYQIGKIDPLTLPTEQKHVETTPHKPTPSLLSFSPLHVLIPLLLLVL 120

Query: 121 AFAFPY 127
           AFAFPY
Sbjct: 121 AFAFPY 126

[Close]

BLAST of Carg13878 vs. ExPASy TrEMBL
Match: A0A6J1KHQ2 (cytochrome b5-like OS=Cucurbita maxima OX=3661 GN=LOC111494495 PE=3 SV=1)

HSP 1 Score: 243.0 bits (619), Expect = 6.5e-61
Identity = 117/126 (92.86%), Postives = 124/126 (98.41%), Query Frame = 0


Query: 1   MDSNSKLFTFDEVAKHNHEKDCWLIISGYVYDVTSYLPDHPGGGELLVLAVEKDATFDFK 60
           MDSNSK++TF+EVAKHNHEKDCWLIISGYVYDVTSYLPDHPGG ELLVL VEKDATFDFK
Sbjct: 1   MDSNSKIYTFNEVAKHNHEKDCWLIISGYVYDVTSYLPDHPGGSELLVLTVEKDATFDFK 60

Query: 61  SVGHSETAQEMMKKYQIGKIDPLTLPTEQKHVETTPHEPTPSLLSFSPLHVLIPLLLLVL 120
           SVGHSETA+EMMKKYQIGKIDPLTLPTEQK+VETTP++PTPSLLSFSPLHVLIPLLLLVL
Sbjct: 61  SVGHSETAREMMKKYQIGKIDPLTLPTEQKYVETTPYKPTPSLLSFSPLHVLIPLLLLVL 120

Query: 121 AFAFPY 127
           AFAFPY
Sbjct: 121 AFAFPY 126

[Close]

BLAST of Carg13878 vs. ExPASy TrEMBL
Match: A0A1S3CAE5 (cytochrome b5 OS=Cucumis melo OX=3656 GN=LOC103498784 PE=3 SV=1)

HSP 1 Score: 215.3 bits (547), Expect = 1.5e-52
Identity = 101/126 (80.16%), Postives = 116/126 (92.06%), Query Frame = 0


Query: 1   MDSNSKLFTFDEVAKHNHEKDCWLIISGYVYDVTSYLPDHPGGGELLVLAVEKDATFDFK 60
           MDSNSK+++FDEVAKHNH+KDCWLIISGYVYDVTS+LPDHPGG ELL+LAVEKDATFDFK
Sbjct: 1   MDSNSKVYSFDEVAKHNHQKDCWLIISGYVYDVTSFLPDHPGGDELLLLAVEKDATFDFK 60

Query: 61  SVGHSETAQEMMKKYQIGKIDPLTLPTEQKHVETTPHEPTPSLLSFSPLHVLIPLLLLVL 120
           SVGHSE AQE MK Y+IGKID  TLP +Q +VE+TP++PTPSL SFSP+HVL+P+LLL L
Sbjct: 61  SVGHSELAQEKMKMYRIGKIDMSTLPKKQNYVESTPYQPTPSLFSFSPIHVLLPILLLAL 120

Query: 121 AFAFPY 127
           AFAFPY
Sbjct: 121 AFAFPY 126

[Close]

BLAST of Carg13878 vs. ExPASy TrEMBL
Match: A0A0A0KA20 (Cytochrome b5 heme-binding domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_6G107940 PE=3 SV=1)

HSP 1 Score: 207.2 bits (526), Expect = 4.0e-50
Identity = 98/126 (77.78%), Postives = 113/126 (89.68%), Query Frame = 0


Query: 1   MDSNSKLFTFDEVAKHNHEKDCWLIISGYVYDVTSYLPDHPGGGELLVLAVEKDATFDFK 60
           MDSNSK+++FDEVAKHN +KDCWLIISGYVYDVTS+LPDHPGG ELL+LAVEKDATFDFK
Sbjct: 1   MDSNSKVYSFDEVAKHNQQKDCWLIISGYVYDVTSFLPDHPGGDELLLLAVEKDATFDFK 60

Query: 61  SVGHSETAQEMMKKYQIGKIDPLTLPTEQKHVETTPHEPTPSLLSFSPLHVLIPLLLLVL 120
           SVGHSE A E MK YQIGKID  TLP +QK+VE+ P++ TPSL SFSP+H+L+P+LLL L
Sbjct: 61  SVGHSELAHEKMKMYQIGKIDMSTLPEKQKYVESAPYQRTPSLFSFSPIHLLLPILLLAL 120

Query: 121 AFAFPY 127
           AFAFPY
Sbjct: 121 AFAFPY 126

[Close]

BLAST of Carg13878 vs. ExPASy TrEMBL
Match: W9RDL9 (Cytochrome b5, seed isoform OS=Morus notabilis OX=981085 GN=L484_023457 PE=3 SV=1)

HSP 1 Score: 140.2 bits (352), Expect = 5.9e-30
Identity = 72/130 (55.38%), Postives = 94/130 (72.31%), Query Frame = 0


Query: 1   MDSNSKLFTFDEVAKHNHEKDCWLIISGYVYDVTSYLPDHPGGGELLVLAVEKDATFDFK 60
           M  +SK+F FDEVAKHNH+KDCW+IISG VY+VT +L +HPGG E+L+LA EKDAT DF+
Sbjct: 1   MARDSKVFAFDEVAKHNHKKDCWIIISGKVYNVTEFLEEHPGGDEVLLLAAEKDATDDFE 60

Query: 61  SVGHSETAQEMMKKYQIGKIDPLTLPTEQKHVETTPHEPTPSLL----SFSPLHVLIPLL 120
            VGHS+TA+EMM+KY +GK+D  TLP E+   +  P  P+ S      S   L  L+PLL
Sbjct: 61  DVGHSDTAREMMEKYYVGKVDVSTLP-EKPSYKPPPQPPSQSNQSSGGSVKILQFLLPLL 120

Query: 121 LLVLAFAFPY 127
           +L +AFA  +
Sbjct: 121 ILAIAFALHF 129

[Close]

BLAST of Carg13878 vs. TAIR 10
Match: AT5G53560.1 (cytochrome B5 isoform E )

HSP 1 Score: 119.8 bits (299), Expect = 1.6e-27
Identity = 63/127 (49.61%), Postives = 87/127 (68.50%), Query Frame = 0


Query: 1   MDSNSKLFTFDEVAKHNHEKDCWLIISGYVYDVTSYLPDHPGGGELLVLAVEKDATFDFK 60
           M S+ K+ +F+EV+KHN  KDCWLIISG VYDVT ++ DHPGG E+L+ +  KDAT DF+
Sbjct: 1   MSSDRKVLSFEEVSKHNKTKDCWLIISGKVYDVTPFMDDHPGGDEVLLSSTGKDATNDFE 60

Query: 61  SVGHSETAQEMMKKYQIGKIDPLTLPTEQKHVETTPHEP------TPSLLSFSPLHVLIP 120
            VGHS+TA++MM KY IG+ID  ++P  + +V   P +P      TP  +    L  L+P
Sbjct: 61  DVGHSDTARDMMDKYFIGEIDSSSVPATRTYV--APQQPAYNQDKTPEFI-IKILQFLVP 120

Query: 121 LLLLVLA 122
           +L+L LA
Sbjct: 121 ILILGLA 124

[Close]

BLAST of Carg13878 vs. TAIR 10
Match: AT2G32720.1 (cytochrome B5 isoform B )

HSP 1 Score: 116.3 bits (290), Expect = 1.8e-26
Identity = 58/126 (46.03%), Postives = 84/126 (66.67%), Query Frame = 0


Query: 1   MDSNSKLFTFDEVAKHNHEKDCWLIISGYVYDVTSYLPDHPGGGELLVLAVEKDATFDFK 60
           M   +K+FT  EV++HN   DCW++I+G VY+VT +L DHPGG ++L+ +  KDAT DF+
Sbjct: 1   MGDEAKIFTLSEVSEHNQAHDCWIVINGKVYNVTKFLEDHPGGDDVLLSSTGKDATDDFE 60

Query: 61  SVGHSETAQEMMKKYQIGKIDPLTLPTEQKHVETTPHEP-----TPSLLSFSPLHVLIPL 120
            VGHSE+A+EMM++Y +G+IDP T+P + K+  T P +P       S      L  L+PL
Sbjct: 61  DVGHSESAREMMEQYYVGEIDPTTIPKKVKY--TPPKQPHYNQDKTSEFIIKLLQFLVPL 120

Query: 121 LLLVLA 122
            +L LA
Sbjct: 121 AILGLA 124

[Close]

BLAST of Carg13878 vs. TAIR 10
Match: AT5G48810.1 (cytochrome B5 isoform D )

HSP 1 Score: 113.6 bits (283), Expect = 1.1e-25
Identity = 58/131 (44.27%), Postives = 81/131 (61.83%), Query Frame = 0


Query: 1   MDSNSKLFTFDEVAKHNHEKDCWLIISGYVYDVTSYLPDHPGGGELLVLAVEKDATFDFK 60
           M  + K+FT  EV++H+  KDCW++I G VYDVT +L DHPGG E+++ +  KDAT DF+
Sbjct: 1   MGGDGKVFTLSEVSQHSSAKDCWIVIDGKVYDVTKFLDDHPGGDEVILTSTGKDATDDFE 60

Query: 61  SVGHSETAQEMMKKYQIGKIDPLTLPTEQKHVETTPHEPTPSLLSFSP-----LHVLIPL 120
            VGHS TA+ M+ +Y +G ID  T+P + K V  T  +   +    S      L  L+PL
Sbjct: 61  DVGHSSTAKAMLDEYYVGDIDTATVPVKAKFVPPTSTKAVATQDKSSDFVIKLLQFLVPL 120

Query: 121 LLLVLAFAFPY 127
           L+L LAF   Y
Sbjct: 121 LILGLAFGIRY 131

[Close]

BLAST of Carg13878 vs. TAIR 10
Match: AT2G46650.1 (cytochrome B5 isoform C )

HSP 1 Score: 105.9 bits (263), Expect = 2.4e-23
Identity = 53/127 (41.73%), Postives = 81/127 (63.78%), Query Frame = 0


Query: 5   SKLFTFDEVAKHNHEKDCWLIISGYVYDVTSYLPDHPGGGELLVLAVEKDATFDFKSVGH 64
           + L +F +VAKH  + DCW++I G VYD+++++ +HPGG  +L+    KDA+ DF+ V H
Sbjct: 2   ANLISFHDVAKHKCKNDCWILIHGKVYDISTFMDEHPGGDNVLLAVTGKDASIDFEDVNH 61

Query: 65  SETAQEMMKKYQIGKIDPLTLPTEQKHV-----ETTPHEPTPSLLSFSPLHVLIPLLLLV 124
           S+ A+E+MKKY IG +D  T+P  Q+++     E+T  E T        L  LIPLL+L 
Sbjct: 62  SKDAKELMKKYCIGDVDQSTVPVTQQYIPPWEKESTAAETTKEESGKKLLIYLIPLLILG 121

Query: 125 LAFAFPY 127
           +AFA  +
Sbjct: 122 VAFALRF 128

[Close]

BLAST of Carg13878 vs. TAIR 10
Match: AT1G26340.1 (cytochrome B5 isoform A )

HSP 1 Score: 100.9 bits (250), Expect = 7.7e-22
Identity = 48/126 (38.10%), Postives = 82/126 (65.08%), Query Frame = 0


Query: 1   MDSNSKLFTFDEVAKHNHEKDCWLIISGYVYDVTSYLPDHPGGGELLVLAVEKDATFDFK 60
           M + +KL++ +E A HN + DCW++I G VYDV+SY+ +HPGG ++L+    KDAT DF+
Sbjct: 1   MPTLTKLYSMEEAATHNKQDDCWVVIDGKVYDVSSYMDEHPGGDDVLLAVAGKDATDDFE 60

Query: 61  SVGHSETAQEMMKKYQIGKIDPLTLP--TEQK-HVETTPHEPTPSLLSFSPLHVLIPLLL 120
             GHS+ A+E+M+KY IG++D  +LP   E K + +  P +    L   +  + ++P+ +
Sbjct: 61  DAGHSKDARELMEKYFIGELDESSLPEIPELKIYKKDQPQDSVQKLFDLTKQYWVVPVSI 120

Query: 121 LVLAFA 124
           + ++ A
Sbjct: 121 ITISVA 126

The following BLAST results are available for this feature:

BLAST of Carg13878 vs. NCBI nr


Match Name	E-value	Identity	Description
KAG7025950.1	3.1e-65	100.00	Cytochrome b5 isoform E [Cucurbita argyrosperma subsp. argyrosperma]	[more]
KAG6593603.1	3.4e-64	98.41	Cycloartenol synthase, partial [Cucurbita argyrosperma subsp. sororia]	[more]
XP_022964486.1	5.4e-62	94.44	cytochrome b5-like [Cucurbita moschata]	[more]
XP_023515254.1	2.7e-61	94.44	cytochrome b5-like [Cucurbita pepo subsp. pepo]	[more]
XP_023000205.1	1.3e-60	92.86	cytochrome b5-like [Cucurbita maxima]	[more]

BLAST of Carg13878 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q42342	2.2e-26	49.61	Cytochrome b5 isoform E OS=Arabidopsis thaliana OX=3702 GN=CYTB5-E PE=1 SV=2	[more]
P49098	5.0e-26	46.56	Cytochrome b5 OS=Nicotiana tabacum OX=4097 PE=2 SV=1	[more]
P49099	6.5e-26	48.80	Cytochrome b5, seed isoform OS=Nicotiana tabacum OX=4097 PE=2 SV=1	[more]
P40934	6.5e-26	48.80	Cytochrome b5 OS=Brassica oleracea var. botrytis OX=3715 GN=CYB5 PE=1 SV=1	[more]
O48845	2.5e-25	46.03	Cytochrome b5 isoform B OS=Arabidopsis thaliana OX=3702 GN=CYTB5-B PE=1 SV=1	[more]

BLAST of Carg13878 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1HJ10	2.6e-62	94.44	cytochrome b5-like OS=Cucurbita moschata OX=3662 GN=LOC111464496 PE=3 SV=1	[more]
A0A6J1KHQ2	6.5e-61	92.86	cytochrome b5-like OS=Cucurbita maxima OX=3661 GN=LOC111494495 PE=3 SV=1	[more]
A0A1S3CAE5	1.5e-52	80.16	cytochrome b5 OS=Cucumis melo OX=3656 GN=LOC103498784 PE=3 SV=1	[more]
A0A0A0KA20	4.0e-50	77.78	Cytochrome b5 heme-binding domain-containing protein OS=Cucumis sativus OX=3659 ...	[more]
W9RDL9	5.9e-30	55.38	Cytochrome b5, seed isoform OS=Morus notabilis OX=981085 GN=L484_023457 PE=3 SV=...	[more]

BLAST of Carg13878 vs. TAIR 10


Match Name	E-value	Identity	Description
AT5G53560.1	1.6e-27	49.61	cytochrome B5 isoform E	[more]
AT2G32720.1	1.8e-26	46.03	cytochrome B5 isoform B	[more]
AT5G48810.1	1.1e-25	44.27	cytochrome B5 isoform D	[more]
AT2G46650.1	2.4e-23	41.73	cytochrome B5 isoform C	[more]
AT1G26340.1	7.7e-22	38.10	cytochrome B5 isoform A	[more]

InterPro

Analysis Name: InterPro Annotations of Silver-seed gourd (SMH-JMG-627) v2
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR001199	Cytochrome b5-like heme/steroid binding domain	PRINTS	PR00363	CYTOCHROMEB5	coord: 40..54 score: 40.51 coord: 30..40 score: 57.02 coord: 55..62 score: 35.8 coord: 68..80 score: 37.41
IPR001199	Cytochrome b5-like heme/steroid binding domain	SMART	SM01117	Cyt_b5_2	coord: 8..81 e-value: 8.1E-27 score: 105.1
IPR001199	Cytochrome b5-like heme/steroid binding domain	PFAM	PF00173	Cyt-b5	coord: 10..80 e-value: 6.8E-22 score: 77.4
IPR001199	Cytochrome b5-like heme/steroid binding domain	PROSITE	PS50255	CYTOCHROME_B5_2	coord: 5..81 score: 26.726944
IPR036400	Cytochrome b5-like heme/steroid binding domain superfamily	GENE3D	3.10.120.10		coord: 1..84 e-value: 8.8E-32 score: 110.9
IPR036400	Cytochrome b5-like heme/steroid binding domain superfamily	SUPERFAMILY	55856	Cytochrome b5-like heme/steroid binding domain	coord: 5..89
None	No IPR available	PANTHER	PTHR19359	CYTOCHROME B5	coord: 1..126
None	No IPR available	PANTHER	PTHR19359:SF81	SUBFAMILY NOT NAMED	coord: 1..126
IPR018506	Cytochrome b5, heme-binding site	PROSITE	PS00191	CYTOCHROME_B5_1	coord: 36..43

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Carg13878-RA	Carg13878-RA	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
cellular_component	GO:0016021	integral component of membrane
cellular_component	GO:0043231	intracellular membrane-bounded organelle
molecular_function	GO:0020037	heme binding
molecular_function	GO:0046872	metal ion binding