Carg13682 (gene) Silver-seed gourd (SMH-JMG-627) v2

Overview


Name	Carg13682
Type	gene
Organism	Cucurbita argyrosperma subsp. argyrosperma cv. SMH-JMG-627 (Silver-seed gourd (SMH-JMG-627) v2)
Description	cytochrome b561 and DOMON domain-containing protein At5g47530-like
Location	Carg_Chr03: 5134022 .. 5134887 (+)
RNA-Seq Expression	Carg13682
Synteny	Carg13682

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGGGTTCCCGTTTCCAGTTCTTCCTAATTGTGAGCCTAGTTTTTGCTCTCTCTAATTCCACGGCGGCGCAACAGGAAAACACCACCTCCGGCCACCACACCCCAGATACCAAAGTAACATCAGCACCAAAATTATGGAAGAGCCCTGGTTTGGGATACTCCACCCTTTTAAGACACGTAAGAATTCAACCATTTCTTTTCTTCTTTTAATTAAGGTAATAATTAAAAAACTAATGGTGAGAGAAGACAGGTTCACGGGGTTCTGAATATCATTGGATGGGGAACTTTGATACCCATTGGGATTGTGATTGCTAGATACTTCAGGGAGGAATTCCCATTGAGATGCGACCGATGGTATTCCGCTCATGCTGTGTGCCAAACATGCGGTTACATTATGGGCACTGTCGGATGGGGCTTCGGCGTGTCGCTCATTAACTCTTCAAACCGCTCTCACGTCCCATTCCTAGTCCTCGGCATTCTCGTCATCCTCCTCACAGCCATTCAAGTTAGTTTCCCTACAAACTCTAAATTGGATTATAAACTCATAATTGTTTATTGACACTGTCCAGGAGAGTTGGCAGATTTGTGGGATATGCGTGCAACAGAAGAGGGAGATCGGAAGGGGGCGGATGGGGGTAATGTGGCACCGTGGCATGGGGTATGTTATATTGGCACTCATAATAGCGGACATATTTGAAGGTATCAATGCGCAGCGCCACCCAAAGAAGTGGCGATGGACTTACGTTGGAATACTATTGGGTGTTGGCGTTGTGGCGGCGGGCTTGGAGGTTTACAGATACATCAAGCTTAAACGCTTCAAACAGGCCATGAAATTAAATGCCAATATGTATTCTCCCACTACTTGA

mRNA sequence

ATGGGTTCCCGTTTCCAGTTCTTCCTAATTGTGAGCCTAGTTTTTGCTCTCTCTAATTCCACGGCGGCGCAACAGGAAAACACCACCTCCGGCCACCACACCCCAGATACCAAAGTAACATCAGCACCAAAATTATGGAAGAGCCCTGGTTTGGGATACTCCACCCTTTTAAGACACGTTCACGGGGTTCTGAATATCATTGGATGGGGAACTTTGATACCCATTGGGATTGTGATTGCTAGATACTTCAGGGAGGAATTCCCATTGAGATGCGACCGATGGTATTCCGCTCATGCTGTGTGCCAAACATGCGGTTACATTATGGGCACTGTCGGATGGGGCTTCGGCGTGTCGCTCATTAACTCTTCAAACCGCTCTCACGTCCCATTCCTAGTCCTCGGCATTCTCGTCATCCTCCTCACAGCCATTCAAATTTGTGGGATATGCGTGCAACAGAAGAGGGAGATCGGAAGGGGGCGGATGGGGGTAATGTGGCACCGTGGCATGGGGTATGTTATATTGGCACTCATAATAGCGGACATATTTGAAGGTATCAATGCGCAGCGCCACCCAAAGAAGTGGCGATGGACTTACGTTGGAATACTATTGGGTGTTGGCGTTGTGGCGGCGGGCTTGGAGGTTTACAGATACATCAAGCTTAAACGCTTCAAACAGGCCATGAAATTAAATGCCAATATGTATTCTCCCACTACTTGA

Coding sequence (CDS)

ATGGGTTCCCGTTTCCAGTTCTTCCTAATTGTGAGCCTAGTTTTTGCTCTCTCTAATTCCACGGCGGCGCAACAGGAAAACACCACCTCCGGCCACCACACCCCAGATACCAAAGTAACATCAGCACCAAAATTATGGAAGAGCCCTGGTTTGGGATACTCCACCCTTTTAAGACACGTTCACGGGGTTCTGAATATCATTGGATGGGGAACTTTGATACCCATTGGGATTGTGATTGCTAGATACTTCAGGGAGGAATTCCCATTGAGATGCGACCGATGGTATTCCGCTCATGCTGTGTGCCAAACATGCGGTTACATTATGGGCACTGTCGGATGGGGCTTCGGCGTGTCGCTCATTAACTCTTCAAACCGCTCTCACGTCCCATTCCTAGTCCTCGGCATTCTCGTCATCCTCCTCACAGCCATTCAAATTTGTGGGATATGCGTGCAACAGAAGAGGGAGATCGGAAGGGGGCGGATGGGGGTAATGTGGCACCGTGGCATGGGGTATGTTATATTGGCACTCATAATAGCGGACATATTTGAAGGTATCAATGCGCAGCGCCACCCAAAGAAGTGGCGATGGACTTACGTTGGAATACTATTGGGTGTTGGCGTTGTGGCGGCGGGCTTGGAGGTTTACAGATACATCAAGCTTAAACGCTTCAAACAGGCCATGAAATTAAATGCCAATATGTATTCTCCCACTACTTGA

Protein sequence

MGSRFQFFLIVSLVFALSNSTAAQQENTTSGHHTPDTKVTSAPKLWKSPGLGYSTLLRHVHGVLNIIGWGTLIPIGIVIARYFREEFPLRCDRWYSAHAVCQTCGYIMGTVGWGFGVSLINSSNRSHVPFLVLGILVILLTAIQICGICVQQKREIGRGRMGVMWHRGMGYVILALIIADIFEGINAQRHPKKWRWTYVGILLGVGVVAAGLEVYRYIKLKRFKQAMKLNANMYSPTT

Homology

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BLAST of Carg13682 vs. NCBI nr
Match: KAG7033962.1 (Cytochrome b561 and DOMON domain-containing protein, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 491.9 bits (1265), Expect = 3.1e-135
Identity = 238/238 (100.00%), Postives = 238/238 (100.00%), Query Frame = 0


Query: 1   MGSRFQFFLIVSLVFALSNSTAAQQENTTSGHHTPDTKVTSAPKLWKSPGLGYSTLLRHV 60
           MGSRFQFFLIVSLVFALSNSTAAQQENTTSGHHTPDTKVTSAPKLWKSPGLGYSTLLRHV
Sbjct: 1   MGSRFQFFLIVSLVFALSNSTAAQQENTTSGHHTPDTKVTSAPKLWKSPGLGYSTLLRHV 60

Query: 61  HGVLNIIGWGTLIPIGIVIARYFREEFPLRCDRWYSAHAVCQTCGYIMGTVGWGFGVSLI 120
           HGVLNIIGWGTLIPIGIVIARYFREEFPLRCDRWYSAHAVCQTCGYIMGTVGWGFGVSLI
Sbjct: 61  HGVLNIIGWGTLIPIGIVIARYFREEFPLRCDRWYSAHAVCQTCGYIMGTVGWGFGVSLI 120

Query: 121 NSSNRSHVPFLVLGILVILLTAIQICGICVQQKREIGRGRMGVMWHRGMGYVILALIIAD 180
           NSSNRSHVPFLVLGILVILLTAIQICGICVQQKREIGRGRMGVMWHRGMGYVILALIIAD
Sbjct: 121 NSSNRSHVPFLVLGILVILLTAIQICGICVQQKREIGRGRMGVMWHRGMGYVILALIIAD 180

Query: 181 IFEGINAQRHPKKWRWTYVGILLGVGVVAAGLEVYRYIKLKRFKQAMKLNANMYSPTT 239
           IFEGINAQRHPKKWRWTYVGILLGVGVVAAGLEVYRYIKLKRFKQAMKLNANMYSPTT
Sbjct: 181 IFEGINAQRHPKKWRWTYVGILLGVGVVAAGLEVYRYIKLKRFKQAMKLNANMYSPTT 238

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BLAST of Carg13682 vs. NCBI nr
Match: KAG6603792.1 (Cytochrome b561 and DOMON domain-containing protein, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 483.8 bits (1244), Expect = 8.5e-133
Identity = 237/242 (97.93%), Postives = 237/242 (97.93%), Query Frame = 0


Query: 1   MGSRFQFFLIVSLVFALSNSTAAQQENTTSGHHTPDTKVTSAPKLWKSPGLGYSTLLRHV 60
           MGSRFQFFLIVSLVFALSNSTAAQQENTTSGHHTPDTKVTSAPKLWKSPGLGYSTLLRHV
Sbjct: 1   MGSRFQFFLIVSLVFALSNSTAAQQENTTSGHHTPDTKVTSAPKLWKSPGLGYSTLLRHV 60

Query: 61  HGVLNIIGWGTLIPIGIVIARYFREEFPLRCDRWYSAHAVCQTCGYIMGTVGWGFGVSLI 120
           HGVLNIIGWGTLIPIGIVIARYFREEFPLRCDRWYSAHAVCQTCGYIMGTVGWGFGVSLI
Sbjct: 61  HGVLNIIGWGTLIPIGIVIARYFREEFPLRCDRWYSAHAVCQTCGYIMGTVGWGFGVSLI 120

Query: 121 NSSNRSHVPFLVLGILVILLTAI----QICGICVQQKREIGRGRMGVMWHRGMGYVILAL 180
           NSSNRSHVPFLVLGILVILLTAI    QICGICVQQKRE GRGRMGVMWHRGMGYVILAL
Sbjct: 121 NSSNRSHVPFLVLGILVILLTAIQESWQICGICVQQKRESGRGRMGVMWHRGMGYVILAL 180

Query: 181 IIADIFEGINAQRHPKKWRWTYVGILLGVGVVAAGLEVYRYIKLKRFKQAMKLNANMYSP 239
           IIADIFEGINAQRHPKKWRWTYVGILLGVGVVAAGLEVYRYIKLKRFKQAMKLNANMYSP
Sbjct: 181 IIADIFEGINAQRHPKKWRWTYVGILLGVGVVAAGLEVYRYIKLKRFKQAMKLNANMYSP 240

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BLAST of Carg13682 vs. NCBI nr
Match: XP_022949945.1 (cytochrome b561 and DOMON domain-containing protein At5g47530-like [Cucurbita moschata])

HSP 1 Score: 479.6 bits (1233), Expect = 1.6e-131
Identity = 231/238 (97.06%), Postives = 234/238 (98.32%), Query Frame = 0


Query: 1   MGSRFQFFLIVSLVFALSNSTAAQQENTTSGHHTPDTKVTSAPKLWKSPGLGYSTLLRHV 60
           MGSRFQFFLIVSLVFALSNSTAAQQENTTSGHHTPDTKVTSAPKLWKSPGLGYSTLLRHV
Sbjct: 1   MGSRFQFFLIVSLVFALSNSTAAQQENTTSGHHTPDTKVTSAPKLWKSPGLGYSTLLRHV 60

Query: 61  HGVLNIIGWGTLIPIGIVIARYFREEFPLRCDRWYSAHAVCQTCGYIMGTVGWGFGVSLI 120
           HGVLNIIGWGTLIPIGIVIARYFREEFPLRCDRWYSAHAVCQTCGYIMGTVGWGFGVSLI
Sbjct: 61  HGVLNIIGWGTLIPIGIVIARYFREEFPLRCDRWYSAHAVCQTCGYIMGTVGWGFGVSLI 120

Query: 121 NSSNRSHVPFLVLGILVILLTAIQICGICVQQKREIGRGRMGVMWHRGMGYVILALIIAD 180
           NSSNRSHVPFLVLGILVI+LTAIQICGICVQQKRE GRGRMGV+WHRGMGYVILALIIAD
Sbjct: 121 NSSNRSHVPFLVLGILVIVLTAIQICGICVQQKRESGRGRMGVVWHRGMGYVILALIIAD 180

Query: 181 IFEGINAQRHPKKWRWTYVGILLGVGVVAAGLEVYRYIKLKRFKQAMKLNANMYSPTT 239
           IFEGINAQRHPKKWRW YVGILL +GVVAAGLEVYRYIKLKRFKQAMKLNANMYSP T
Sbjct: 181 IFEGINAQRHPKKWRWAYVGILLALGVVAAGLEVYRYIKLKRFKQAMKLNANMYSPAT 238

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BLAST of Carg13682 vs. NCBI nr
Match: XP_023544277.1 (cytochrome b561 and DOMON domain-containing protein At5g47530-like isoform X1 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 459.9 bits (1182), Expect = 1.3e-125
Identity = 222/238 (93.28%), Postives = 229/238 (96.22%), Query Frame = 0


Query: 1   MGSRFQFFLIVSLVFALSNSTAAQQENTTSGHHTPDTKVTSAPKLWKSPGLGYSTLLRHV 60
           MGSRFQFFLI+SL+FALS+STAAQQENTTSGHHTPDTKVTSAPK+WKSPGLGYSTLLR V
Sbjct: 1   MGSRFQFFLILSLIFALSDSTAAQQENTTSGHHTPDTKVTSAPKIWKSPGLGYSTLLRQV 60

Query: 61  HGVLNIIGWGTLIPIGIVIARYFREEFPLRCDRWYSAHAVCQTCGYIMGTVGWGFGVSLI 120
           HGVLNIIGWGTLIPIGIVIARYFREEFPL+CDRWYSAHAVCQTCGYIMGTVGWGFGVSLI
Sbjct: 61  HGVLNIIGWGTLIPIGIVIARYFREEFPLKCDRWYSAHAVCQTCGYIMGTVGWGFGVSLI 120

Query: 121 NSSNRSHVPFLVLGILVILLTAIQICGICVQQKREIGRGRMGVMWHRGMGYVILALIIAD 180
           NSSNRSHVPFLVLGILVILLTAIQICGICVQQK+E GR     MWHRGMGYVILALIIAD
Sbjct: 121 NSSNRSHVPFLVLGILVILLTAIQICGICVQQKKESGRRGRWEMWHRGMGYVILALIIAD 180

Query: 181 IFEGINAQRHPKKWRWTYVGILLGVGVVAAGLEVYRYIKLKRFKQAMKLNANMYSPTT 239
           IFEGINAQRHPKKWRWTYVGILL +GVVAAGLEVYRYIKLKRFKQAMKLNANMYS TT
Sbjct: 181 IFEGINAQRHPKKWRWTYVGILLALGVVAAGLEVYRYIKLKRFKQAMKLNANMYSSTT 238

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BLAST of Carg13682 vs. NCBI nr
Match: XP_023544278.1 (cytochrome b561 and DOMON domain-containing protein At5g47530-like isoform X2 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 459.5 bits (1181), Expect = 1.7e-125
Identity = 222/238 (93.28%), Postives = 229/238 (96.22%), Query Frame = 0


Query: 1   MGSRFQFFLIVSLVFALSNSTAAQQENTTSGHHTPDTKVTSAPKLWKSPGLGYSTLLRHV 60
           MGSRFQFFLI+SL+FALS+STAAQQENTTSGHHTPDTKVTSAPK+WKSPGLGYSTLLR V
Sbjct: 1   MGSRFQFFLILSLIFALSDSTAAQQENTTSGHHTPDTKVTSAPKIWKSPGLGYSTLLRXV 60

Query: 61  HGVLNIIGWGTLIPIGIVIARYFREEFPLRCDRWYSAHAVCQTCGYIMGTVGWGFGVSLI 120
           HGVLNIIGWGTLIPIGIVIARYFREEFPL+CDRWYSAHAVCQTCGYIMGTVGWGFGVSLI
Sbjct: 61  HGVLNIIGWGTLIPIGIVIARYFREEFPLKCDRWYSAHAVCQTCGYIMGTVGWGFGVSLI 120

Query: 121 NSSNRSHVPFLVLGILVILLTAIQICGICVQQKREIGRGRMGVMWHRGMGYVILALIIAD 180
           NSSNRSHVPFLVLGILVILLTAIQICGICVQQK+E GR     MWHRGMGYVILALIIAD
Sbjct: 121 NSSNRSHVPFLVLGILVILLTAIQICGICVQQKKESGRRGRWEMWHRGMGYVILALIIAD 180

Query: 181 IFEGINAQRHPKKWRWTYVGILLGVGVVAAGLEVYRYIKLKRFKQAMKLNANMYSPTT 239
           IFEGINAQRHPKKWRWTYVGILL +GVVAAGLEVYRYIKLKRFKQAMKLNANMYS TT
Sbjct: 181 IFEGINAQRHPKKWRWTYVGILLALGVVAAGLEVYRYIKLKRFKQAMKLNANMYSSTT 238

[Close]

BLAST of Carg13682 vs. ExPASy Swiss-Prot
Match: Q9FGK4 (Cytochrome b561 and DOMON domain-containing protein At5g47530 OS=Arabidopsis thaliana OX=3702 GN=At5g47530 PE=2 SV=1)

HSP 1 Score: 108.2 bits (269), Expect = 1.3e-22
Identity = 59/181 (32.60%), Postives = 98/181 (54.14%), Query Frame = 0


Query: 48  SPGLGYSTLL--RHVHGVLNIIGWGTLIPIGIVIARYFREEFPLRCDRWYSAHAVCQTCG 107
           S G G ++ L  R++HG+LN + WG ++PIG +IARY +         W+  H  CQ+  
Sbjct: 196 STGAGGASKLRKRNIHGILNGVSWGIMMPIGAIIARYLKVS-KSADPAWFYLHVFCQSSA 255

Query: 108 YIMGTVGWGFGVSLINSSNRSHVPF-LVLGILVILLTAIQICGICVQQKREIGRGRMGVM 167
           YI+G  GW  G+ L N S      F   +GI +  L  IQ+  + ++ K E        +
Sbjct: 256 YIIGVAGWATGLKLGNESAGIQFTFHRAVGIALFCLATIQVFAMFLRPKPEHKYRVYWNI 315

Query: 168 WHRGMGYVILALIIADIFEGINAQRHPKKWRWTYVGILLGVGVVAAGLEVYR-YIKLKRF 225
           +H  +GY ++ L + ++F+G++     K+WR  Y  I++ +G+VA  LE +  Y+ +KR 
Sbjct: 316 YHHTVGYSVIILAVVNVFKGLDILSPEKQWRNAYTAIIVVLGIVAVVLEGFTWYVVIKRG 375

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BLAST of Carg13682 vs. ExPASy Swiss-Prot
Match: Q8VYH6 (Cytochrome b561 and DOMON domain-containing protein At4g17280 OS=Arabidopsis thaliana OX=3702 GN=At4g17280 PE=2 SV=1)

HSP 1 Score: 105.5 bits (262), Expect = 8.3e-22
Identity = 57/183 (31.15%), Postives = 97/183 (53.01%), Query Frame = 0


Query: 50  GLGYSTLL--RHVHGVLNIIGWGTLIPIGIVIARYFREEFPLRCDRWYSAHAVCQTCGYI 109
           G G S+ L  R++HG+LN + WG ++P+G +IARY R         W+  H  CQ   YI
Sbjct: 206 GAGGSSKLRKRNIHGILNGVSWGIMMPLGAIIARYLRVA-KSADPAWFYIHVFCQASAYI 265

Query: 110 MGTVGWGFGVSL-INSSNRSHVPFLVLGILVILLTAIQICGICVQQKREIGRGRMGVMWH 169
           +G  GW  G+ L  +S    +     +GI +  L  +Q+  + ++ K E        ++H
Sbjct: 266 IGVAGWATGLKLGGDSPGIQYSTHRAIGIALFSLATVQVFAMFLRPKPEHKHRLYWNIYH 325

Query: 170 RGMGYVILALIIADIFEGINAQRHPKKWRWTYVGILLGVGVVAAGLEVYR-YIKLKRFKQ 229
             +GY I+ L + ++F+G+      K+W+  Y+GI++ + +VA  LE +  Y+ +KR K 
Sbjct: 326 HTIGYTIIILGVVNVFKGLGILSPKKQWKNAYIGIIVVLAIVATLLEAFTWYVVIKRRKL 385

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BLAST of Carg13682 vs. ExPASy Swiss-Prot
Match: Q9FKH6 (Cytochrome b561 and DOMON domain-containing protein At5g35735 OS=Arabidopsis thaliana OX=3702 GN=At5g35735 PE=2 SV=1)

HSP 1 Score: 104.0 bits (258), Expect = 2.4e-21
Identity = 56/176 (31.82%), Postives = 86/176 (48.86%), Query Frame = 0


Query: 50  GLGYSTLLRHVHGVLNIIGWGTLIPIGIVIARYFREEFPLRCDRWYSAHAVCQTCGYIMG 109
           G G     R+ HGVLN + WG L+P+G ++ARY +         W+  H   Q  GY++G
Sbjct: 200 GSGDRLRKRNTHGVLNAVSWGVLMPMGAMMARYMK---VFADPTWFYLHIAFQVSGYVIG 259

Query: 110 TVGWGFGVSLINSS-NRSHVPFLVLGILVILLTAIQICGICVQQKREIGRGRMGVMWHRG 169
             GW  G+ L N S   S+     LGI +     +Q+  + V+ K +        ++H  
Sbjct: 260 VAGWATGIKLGNDSPGTSYSTHRNLGIALFTFATLQVFALLVRPKPDHKYRTYWNVYHHT 319

Query: 170 MGYVILALIIADIFEGINAQRHPKKWRWTYVGILLGVGVVAAGLEVYRYIKLKRFK 225
           +GY  + L I +IF+G +      KWRW Y+GIL+ +G     LE   +  + R K
Sbjct: 320 VGYTTIILSIVNIFKGFDILDPEDKWRWAYIGILIFLGACVLILEPLTWFIVLRRK 372

[Close]

BLAST of Carg13682 vs. ExPASy Swiss-Prot
Match: Q9LSE7 (Cytochrome b561 and DOMON domain-containing protein At3g25290 OS=Arabidopsis thaliana OX=3702 GN=At3g25290 PE=2 SV=1)

HSP 1 Score: 102.1 bits (253), Expect = 9.2e-21
Identity = 52/168 (30.95%), Postives = 88/168 (52.38%), Query Frame = 0


Query: 58  RHVHGVLNIIGWGTLIPIGIVIARYFREEFPLRCDRWYSAHAVCQTCGYIMGTVGWGFGV 117
           R++HG+LN + WG L PIG +IARY R  F      W+  H  CQ   Y++G  GW  G+
Sbjct: 217 RNIHGILNAVSWGILFPIGAIIARYMR-VFDSADPAWFYLHVSCQFSAYVIGVAGWATGL 276

Query: 118 SLINSSNRSHV-PFLVLGILVILLTAIQICGICVQQKREIGRGRMGVMWHRGMGYVILAL 177
            L N S          +GI +  L  IQ+  + ++ K++        ++H G+GY IL L
Sbjct: 277 KLGNESEGIRFSAHRNIGIALFTLATIQMFAMLLRPKKDHKYRFYWNIYHHGVGYAILTL 336

Query: 178 IIADIFEGINAQRHPKKWRWTYVGILLGVGVVAAGLEVYRYIKLKRFK 225
            I ++F+G+N  +    ++  Y+ ++  +G +A  LE   ++ + + K
Sbjct: 337 GIINVFKGLNILKPQDTYKTAYIAVIAVLGGIALLLEAITWVVVLKRK 383

[Close]

BLAST of Carg13682 vs. ExPASy Swiss-Prot
Match: Q9SV71 (Cytochrome b561 and DOMON domain-containing protein At4g12980 OS=Arabidopsis thaliana OX=3702 GN=At4g12980 PE=2 SV=1)

HSP 1 Score: 94.0 bits (232), Expect = 2.5e-18
Identity = 48/170 (28.24%), Postives = 89/170 (52.35%), Query Frame = 0


Query: 58  RHVHGVLNIIGWGTLIPIGIVIARYFREEFPLRCDRWYSAHAVCQTCGYIMGTVGWGFGV 117
           R++HG+LN + WG L PIG +IARY R  F      W+  H  CQ   Y++G  GW  G+
Sbjct: 218 RNIHGILNAVSWGLLFPIGAMIARYMR-IFESADPAWFYLHVSCQFSAYVIGVAGWATGL 277

Query: 118 SL-INSSNRSHVPFLVLGILVILLTAIQICGICVQQKREIGRGRMGVMWHRGMGYVILAL 177
            L   S    +     +GI +  +  +Q+  + ++ +++     +  ++H G+GY IL L
Sbjct: 278 KLGSESKGIQYNTHRNIGICLFSIATLQMFAMLLRPRKDHKFRFVWNIYHHGVGYSILIL 337

Query: 178 IIADIFEGINAQRHPKKWRWTYVGILLGVGVVAAGLEVYRYIKLKRFKQA 227
            I ++F+G++       ++  Y+ ++  +G +   LEV  ++ + + K A
Sbjct: 338 GIINVFKGLSILNPKHTYKTAYIAVIGTLGGITLLLEVVTWVIVLKRKSA 386

[Close]

BLAST of Carg13682 vs. ExPASy TrEMBL
Match: A0A6J1GE73 (cytochrome b561 and DOMON domain-containing protein At5g47530-like OS=Cucurbita moschata OX=3662 GN=LOC111453186 PE=4 SV=1)

HSP 1 Score: 479.6 bits (1233), Expect = 7.8e-132
Identity = 231/238 (97.06%), Postives = 234/238 (98.32%), Query Frame = 0


Query: 1   MGSRFQFFLIVSLVFALSNSTAAQQENTTSGHHTPDTKVTSAPKLWKSPGLGYSTLLRHV 60
           MGSRFQFFLIVSLVFALSNSTAAQQENTTSGHHTPDTKVTSAPKLWKSPGLGYSTLLRHV
Sbjct: 1   MGSRFQFFLIVSLVFALSNSTAAQQENTTSGHHTPDTKVTSAPKLWKSPGLGYSTLLRHV 60

Query: 61  HGVLNIIGWGTLIPIGIVIARYFREEFPLRCDRWYSAHAVCQTCGYIMGTVGWGFGVSLI 120
           HGVLNIIGWGTLIPIGIVIARYFREEFPLRCDRWYSAHAVCQTCGYIMGTVGWGFGVSLI
Sbjct: 61  HGVLNIIGWGTLIPIGIVIARYFREEFPLRCDRWYSAHAVCQTCGYIMGTVGWGFGVSLI 120

Query: 121 NSSNRSHVPFLVLGILVILLTAIQICGICVQQKREIGRGRMGVMWHRGMGYVILALIIAD 180
           NSSNRSHVPFLVLGILVI+LTAIQICGICVQQKRE GRGRMGV+WHRGMGYVILALIIAD
Sbjct: 121 NSSNRSHVPFLVLGILVIVLTAIQICGICVQQKRESGRGRMGVVWHRGMGYVILALIIAD 180

Query: 181 IFEGINAQRHPKKWRWTYVGILLGVGVVAAGLEVYRYIKLKRFKQAMKLNANMYSPTT 239
           IFEGINAQRHPKKWRW YVGILL +GVVAAGLEVYRYIKLKRFKQAMKLNANMYSP T
Sbjct: 181 IFEGINAQRHPKKWRWAYVGILLALGVVAAGLEVYRYIKLKRFKQAMKLNANMYSPAT 238

[Close]

BLAST of Carg13682 vs. ExPASy TrEMBL
Match: A0A6J1INM1 (cytochrome b561 and DOMON domain-containing protein At5g47530-like isoform X2 OS=Cucurbita maxima OX=3661 GN=LOC111478057 PE=4 SV=1)

HSP 1 Score: 446.8 bits (1148), Expect = 5.6e-122
Identity = 216/238 (90.76%), Postives = 225/238 (94.54%), Query Frame = 0


Query: 1   MGSRFQFFLIVSLVFALSNSTAAQQENTTSGHHTPDTKVTSAPKLWKSPGLGYSTLLRHV 60
           MGSRFQFFLI+SLVFALS+STA QQ+NTT GHHTPDTKVTSAPK+WKSPGLGYSTLLRHV
Sbjct: 1   MGSRFQFFLILSLVFALSDSTAVQQQNTT-GHHTPDTKVTSAPKIWKSPGLGYSTLLRHV 60

Query: 61  HGVLNIIGWGTLIPIGIVIARYFREEFPLRCDRWYSAHAVCQTCGYIMGTVGWGFGVSLI 120
           HGVLNIIGWGTLIPIGIVIARYFREEFPLRCDRWYSAHAVCQTCGYIMGTVGWGFGVSLI
Sbjct: 61  HGVLNIIGWGTLIPIGIVIARYFREEFPLRCDRWYSAHAVCQTCGYIMGTVGWGFGVSLI 120

Query: 121 NSSNRSHVPFLVLGILVILLTAIQICGICVQQKREIGRGRMGVMWHRGMGYVILALIIAD 180
           NSSNRSH+PFLVLGIL+I+LTAIQICGICVQQK+E GR     MWHRGMGYVILALIIAD
Sbjct: 121 NSSNRSHIPFLVLGILIIVLTAIQICGICVQQKKESGRRGRWEMWHRGMGYVILALIIAD 180

Query: 181 IFEGINAQRHPKKWRWTYVGILLGVGVVAAGLEVYRYIKLKRFKQAMKLNANMYSPTT 239
           IFEGINAQRHPKKWRW YVGILL + VVAA LEVY YIKLKRFKQAMKLNANMYSPTT
Sbjct: 181 IFEGINAQRHPKKWRWAYVGILLALVVVAAALEVYTYIKLKRFKQAMKLNANMYSPTT 237

[Close]

BLAST of Carg13682 vs. ExPASy TrEMBL
Match: A0A6J1ISN4 (cytochrome b561 and DOMON domain-containing protein At4g17280-like isoform X1 OS=Cucurbita maxima OX=3661 GN=LOC111478057 PE=4 SV=1)

HSP 1 Score: 441.4 bits (1134), Expect = 2.4e-120
Identity = 216/241 (89.63%), Postives = 225/241 (93.36%), Query Frame = 0


Query: 1   MGSRFQFFLIVSLVFALSNSTAAQQENTTSGHHTPDTKVTSAPKLWKSPGLGYSTLLRHV 60
           MGSRFQFFLI+SLVFALS+STA QQ+NTT GHHTPDTKVTSAPK+WKSPGLGYSTLLRHV
Sbjct: 1   MGSRFQFFLILSLVFALSDSTAVQQQNTT-GHHTPDTKVTSAPKIWKSPGLGYSTLLRHV 60

Query: 61  HGVLNIIGWGTLIPIGIVIARYFREEFPLRCDRWYSAHAVCQTCGYIMGTVGWGFGVSLI 120
           HGVLNIIGWGTLIPIGIVIARYFREEFPLRCDRWYSAHAVCQTCGYIMGTVGWGFGVSLI
Sbjct: 61  HGVLNIIGWGTLIPIGIVIARYFREEFPLRCDRWYSAHAVCQTCGYIMGTVGWGFGVSLI 120

Query: 121 NSSNRSHVPFLVLGILVILLTAI---QICGICVQQKREIGRGRMGVMWHRGMGYVILALI 180
           NSSNRSH+PFLVLGIL+I+LTAI   QICGICVQQK+E GR     MWHRGMGYVILALI
Sbjct: 121 NSSNRSHIPFLVLGILIIVLTAIQSWQICGICVQQKKESGRRGRWEMWHRGMGYVILALI 180

Query: 181 IADIFEGINAQRHPKKWRWTYVGILLGVGVVAAGLEVYRYIKLKRFKQAMKLNANMYSPT 239
           IADIFEGINAQRHPKKWRW YVGILL + VVAA LEVY YIKLKRFKQAMKLNANMYSPT
Sbjct: 181 IADIFEGINAQRHPKKWRWAYVGILLALVVVAAALEVYTYIKLKRFKQAMKLNANMYSPT 240

[Close]

BLAST of Carg13682 vs. ExPASy TrEMBL
Match: A0A1S3B066 (cytochrome b561 and DOMON domain-containing protein At4g17280-like isoform X1 OS=Cucumis melo OX=3656 GN=LOC103484629 PE=4 SV=1)

HSP 1 Score: 346.7 bits (888), Expect = 7.9e-92
Identity = 173/250 (69.20%), Postives = 202/250 (80.80%), Query Frame = 0


Query: 1   MGSRFQFFLIV-SLVFALSN--------STAAQQENTTSGHHTPDTKVTSAPKLWKSPGL 60
           MGSRF  FLI+  L+ ALS+        +TA QQ N+TSG H PD KVTS  K+WK+PGL
Sbjct: 1   MGSRFHLFLIILCLLLALSSIGYSVPTEATAEQQANSTSGLHNPDAKVTSPSKIWKNPGL 60

Query: 61  GYSTLLRHVHGVLNIIGWGTLIPIGIVIARYFREEFPLRCDRWYSAHAVCQTCGYIMGTV 120
           GY+TLLRHVHGVLNIIGWGTLIPIGI++ RYFR+EFP+RCD+WYS HAVCQTCGYIMGTV
Sbjct: 61  GYTTLLRHVHGVLNIIGWGTLIPIGIIVGRYFRQEFPIRCDQWYSVHAVCQTCGYIMGTV 120

Query: 121 GWGFGVSLINSSN-RSHVPFLVLGILVILLTAIQIC-GICVQQKREIG-RGRMGVMWHRG 180
           GW FGVS+++SS+ RSH+PFLVLGI +ILLT IQI   +C+Q K+E G R R   ++H  
Sbjct: 121 GWAFGVSVLHSSSKRSHLPFLVLGIFIILLTTIQIMFAVCMQPKKESGARRRCWEIYHHV 180

Query: 181 MGYVILALIIADIFEGINAQRHPKKWRWTYVGILLGVGVVAAGLEVYRYIKLKRFKQAMK 239
           MGYVI+ALII  IFEGINAQRHPKKWRW YVGIL G+ +V A LEV+R  +LK FKQAMK
Sbjct: 181 MGYVIMALIIGVIFEGINAQRHPKKWRWGYVGILSGLAIVGAALEVHRCYRLKLFKQAMK 240

[Close]

BLAST of Carg13682 vs. ExPASy TrEMBL
Match: A0A0A0KHA4 (Cytochrome b561 domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_6G513490 PE=4 SV=1)

HSP 1 Score: 335.9 bits (860), Expect = 1.4e-88
Identity = 174/250 (69.60%), Postives = 197/250 (78.80%), Query Frame = 0


Query: 1   MGSRFQFFLIV-SLVFALSN--------STAAQQENTTSGHHTPDTKVTSAPKLWKSPGL 60
           MGSRF  FLI+ SLV ALS+        +T+ QQ N+TSG H PD KVTS  K  K+PGL
Sbjct: 1   MGSRFCLFLIILSLVMALSSVGYSAPTEATSEQQANSTSGLHDPDAKVTSPSKTLKNPGL 60

Query: 61  GYSTLLRHVHGVLNIIGWGTLIPIGIVIARYFREEFPLRCDRWYSAHAVCQTCGYIMGTV 120
           GY TLLRHVHG+LNIIGWGTLIPIGI+I RYFR EFP+RCD+WYS HAVCQTCGYIMGTV
Sbjct: 61  GYVTLLRHVHGILNIIGWGTLIPIGIIIGRYFRHEFPIRCDQWYSIHAVCQTCGYIMGTV 120

Query: 121 GWGFGVSLINSSN-RSHVPFLVLGILVILLTAIQI-CGICVQQKREIG-RGRMGVMWHRG 180
           GW FGVS+++SS+ RS++PFLVLGI +ILLT IQI   ICVQ K+E G R R     H  
Sbjct: 121 GWAFGVSVLHSSSKRSYLPFLVLGIFIILLTTIQIMLAICVQSKKESGERRRCWEKHHHV 180

Query: 181 MGYVILALIIADIFEGINAQRHPKKWRWTYVGILLGVGVVAAGLEVYRYIKLKRFKQAMK 239
           MGYVI+ALII  IFEGINAQRHPKKWRW YVGIL G+ +V A LEV+R  KLK FKQAMK
Sbjct: 181 MGYVIMALIIGVIFEGINAQRHPKKWRWCYVGILSGLAIVGAALEVHRCYKLKLFKQAMK 240

[Close]

BLAST of Carg13682 vs. TAIR 10
Match: AT5G47530.1 (Auxin-responsive family protein )

HSP 1 Score: 108.2 bits (269), Expect = 9.1e-24
Identity = 59/181 (32.60%), Postives = 98/181 (54.14%), Query Frame = 0


Query: 48  SPGLGYSTLL--RHVHGVLNIIGWGTLIPIGIVIARYFREEFPLRCDRWYSAHAVCQTCG 107
           S G G ++ L  R++HG+LN + WG ++PIG +IARY +         W+  H  CQ+  
Sbjct: 196 STGAGGASKLRKRNIHGILNGVSWGIMMPIGAIIARYLKVS-KSADPAWFYLHVFCQSSA 255

Query: 108 YIMGTVGWGFGVSLINSSNRSHVPF-LVLGILVILLTAIQICGICVQQKREIGRGRMGVM 167
           YI+G  GW  G+ L N S      F   +GI +  L  IQ+  + ++ K E        +
Sbjct: 256 YIIGVAGWATGLKLGNESAGIQFTFHRAVGIALFCLATIQVFAMFLRPKPEHKYRVYWNI 315

Query: 168 WHRGMGYVILALIIADIFEGINAQRHPKKWRWTYVGILLGVGVVAAGLEVYR-YIKLKRF 225
           +H  +GY ++ L + ++F+G++     K+WR  Y  I++ +G+VA  LE +  Y+ +KR 
Sbjct: 316 YHHTVGYSVIILAVVNVFKGLDILSPEKQWRNAYTAIIVVLGIVAVVLEGFTWYVVIKRG 375

[Close]

BLAST of Carg13682 vs. TAIR 10
Match: AT4G17280.1 (Auxin-responsive family protein )

HSP 1 Score: 105.5 bits (262), Expect = 5.9e-23
Identity = 57/183 (31.15%), Postives = 97/183 (53.01%), Query Frame = 0


Query: 50  GLGYSTLL--RHVHGVLNIIGWGTLIPIGIVIARYFREEFPLRCDRWYSAHAVCQTCGYI 109
           G G S+ L  R++HG+LN + WG ++P+G +IARY R         W+  H  CQ   YI
Sbjct: 206 GAGGSSKLRKRNIHGILNGVSWGIMMPLGAIIARYLRVA-KSADPAWFYIHVFCQASAYI 265

Query: 110 MGTVGWGFGVSL-INSSNRSHVPFLVLGILVILLTAIQICGICVQQKREIGRGRMGVMWH 169
           +G  GW  G+ L  +S    +     +GI +  L  +Q+  + ++ K E        ++H
Sbjct: 266 IGVAGWATGLKLGGDSPGIQYSTHRAIGIALFSLATVQVFAMFLRPKPEHKHRLYWNIYH 325

Query: 170 RGMGYVILALIIADIFEGINAQRHPKKWRWTYVGILLGVGVVAAGLEVYR-YIKLKRFKQ 229
             +GY I+ L + ++F+G+      K+W+  Y+GI++ + +VA  LE +  Y+ +KR K 
Sbjct: 326 HTIGYTIIILGVVNVFKGLGILSPKKQWKNAYIGIIVVLAIVATLLEAFTWYVVIKRRKL 385

[Close]

BLAST of Carg13682 vs. TAIR 10
Match: AT5G35735.1 (Auxin-responsive family protein )

HSP 1 Score: 104.0 bits (258), Expect = 1.7e-22
Identity = 56/176 (31.82%), Postives = 86/176 (48.86%), Query Frame = 0


Query: 50  GLGYSTLLRHVHGVLNIIGWGTLIPIGIVIARYFREEFPLRCDRWYSAHAVCQTCGYIMG 109
           G G     R+ HGVLN + WG L+P+G ++ARY +         W+  H   Q  GY++G
Sbjct: 200 GSGDRLRKRNTHGVLNAVSWGVLMPMGAMMARYMK---VFADPTWFYLHIAFQVSGYVIG 259

Query: 110 TVGWGFGVSLINSS-NRSHVPFLVLGILVILLTAIQICGICVQQKREIGRGRMGVMWHRG 169
             GW  G+ L N S   S+     LGI +     +Q+  + V+ K +        ++H  
Sbjct: 260 VAGWATGIKLGNDSPGTSYSTHRNLGIALFTFATLQVFALLVRPKPDHKYRTYWNVYHHT 319

Query: 170 MGYVILALIIADIFEGINAQRHPKKWRWTYVGILLGVGVVAAGLEVYRYIKLKRFK 225
           +GY  + L I +IF+G +      KWRW Y+GIL+ +G     LE   +  + R K
Sbjct: 320 VGYTTIILSIVNIFKGFDILDPEDKWRWAYIGILIFLGACVLILEPLTWFIVLRRK 372

[Close]

BLAST of Carg13682 vs. TAIR 10
Match: AT3G25290.1 (Auxin-responsive family protein )

HSP 1 Score: 102.1 bits (253), Expect = 6.5e-22
Identity = 52/168 (30.95%), Postives = 88/168 (52.38%), Query Frame = 0


Query: 58  RHVHGVLNIIGWGTLIPIGIVIARYFREEFPLRCDRWYSAHAVCQTCGYIMGTVGWGFGV 117
           R++HG+LN + WG L PIG +IARY R  F      W+  H  CQ   Y++G  GW  G+
Sbjct: 217 RNIHGILNAVSWGILFPIGAIIARYMR-VFDSADPAWFYLHVSCQFSAYVIGVAGWATGL 276

Query: 118 SLINSSNRSHV-PFLVLGILVILLTAIQICGICVQQKREIGRGRMGVMWHRGMGYVILAL 177
            L N S          +GI +  L  IQ+  + ++ K++        ++H G+GY IL L
Sbjct: 277 KLGNESEGIRFSAHRNIGIALFTLATIQMFAMLLRPKKDHKYRFYWNIYHHGVGYAILTL 336

Query: 178 IIADIFEGINAQRHPKKWRWTYVGILLGVGVVAAGLEVYRYIKLKRFK 225
            I ++F+G+N  +    ++  Y+ ++  +G +A  LE   ++ + + K
Sbjct: 337 GIINVFKGLNILKPQDTYKTAYIAVIAVLGGIALLLEAITWVVVLKRK 383

[Close]

BLAST of Carg13682 vs. TAIR 10
Match: AT3G25290.2 (Auxin-responsive family protein )

HSP 1 Score: 102.1 bits (253), Expect = 6.5e-22
Identity = 52/168 (30.95%), Postives = 88/168 (52.38%), Query Frame = 0


Query: 58  RHVHGVLNIIGWGTLIPIGIVIARYFREEFPLRCDRWYSAHAVCQTCGYIMGTVGWGFGV 117
           R++HG+LN + WG L PIG +IARY R  F      W+  H  CQ   Y++G  GW  G+
Sbjct: 217 RNIHGILNAVSWGILFPIGAIIARYMR-VFDSADPAWFYLHVSCQFSAYVIGVAGWATGL 276

Query: 118 SLINSSNRSHV-PFLVLGILVILLTAIQICGICVQQKREIGRGRMGVMWHRGMGYVILAL 177
            L N S          +GI +  L  IQ+  + ++ K++        ++H G+GY IL L
Sbjct: 277 KLGNESEGIRFSAHRNIGIALFTLATIQMFAMLLRPKKDHKYRFYWNIYHHGVGYAILTL 336

Query: 178 IIADIFEGINAQRHPKKWRWTYVGILLGVGVVAAGLEVYRYIKLKRFK 225
            I ++F+G+N  +    ++  Y+ ++  +G +A  LE   ++ + + K
Sbjct: 337 GIINVFKGLNILKPQDTYKTAYIAVIAVLGGIALLLEAITWVVVLKRK 383

The following BLAST results are available for this feature:

BLAST of Carg13682 vs. NCBI nr


Match Name	E-value	Identity	Description
KAG7033962.1	3.1e-135	100.00	Cytochrome b561 and DOMON domain-containing protein, partial [Cucurbita argyrosp...	[more]
KAG6603792.1	8.5e-133	97.93	Cytochrome b561 and DOMON domain-containing protein, partial [Cucurbita argyrosp...	[more]
XP_022949945.1	1.6e-131	97.06	cytochrome b561 and DOMON domain-containing protein At5g47530-like [Cucurbita mo...	[more]
XP_023544277.1	1.3e-125	93.28	cytochrome b561 and DOMON domain-containing protein At5g47530-like isoform X1 [C...	[more]
XP_023544278.1	1.7e-125	93.28	cytochrome b561 and DOMON domain-containing protein At5g47530-like isoform X2 [C...	[more]

BLAST of Carg13682 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q9FGK4	1.3e-22	32.60	Cytochrome b561 and DOMON domain-containing protein At5g47530 OS=Arabidopsis tha...	[more]
Q8VYH6	8.3e-22	31.15	Cytochrome b561 and DOMON domain-containing protein At4g17280 OS=Arabidopsis tha...	[more]
Q9FKH6	2.4e-21	31.82	Cytochrome b561 and DOMON domain-containing protein At5g35735 OS=Arabidopsis tha...	[more]
Q9LSE7	9.2e-21	30.95	Cytochrome b561 and DOMON domain-containing protein At3g25290 OS=Arabidopsis tha...	[more]
Q9SV71	2.5e-18	28.24	Cytochrome b561 and DOMON domain-containing protein At4g12980 OS=Arabidopsis tha...	[more]

BLAST of Carg13682 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1GE73	7.8e-132	97.06	cytochrome b561 and DOMON domain-containing protein At5g47530-like OS=Cucurbita ...	[more]
A0A6J1INM1	5.6e-122	90.76	cytochrome b561 and DOMON domain-containing protein At5g47530-like isoform X2 OS...	[more]
A0A6J1ISN4	2.4e-120	89.63	cytochrome b561 and DOMON domain-containing protein At4g17280-like isoform X1 OS...	[more]
A0A1S3B066	7.9e-92	69.20	cytochrome b561 and DOMON domain-containing protein At4g17280-like isoform X1 OS...	[more]
A0A0A0KHA4	1.4e-88	69.60	Cytochrome b561 domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_6G51...	[more]

BLAST of Carg13682 vs. TAIR 10


Match Name	E-value	Identity	Description
AT5G47530.1	9.1e-24	32.60	Auxin-responsive family protein	[more]
AT4G17280.1	5.9e-23	31.15	Auxin-responsive family protein	[more]
AT5G35735.1	1.7e-22	31.82	Auxin-responsive family protein	[more]
AT3G25290.1	6.5e-22	30.95	Auxin-responsive family protein	[more]
AT3G25290.2	6.5e-22	30.95	Auxin-responsive family protein	[more]

InterPro

Analysis Name: InterPro Annotations of Silver-seed gourd (SMH-JMG-627) v2
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR006593	Cytochrome b561/ferric reductase transmembrane	SMART	SM00665	561_7	coord: 60..185 e-value: 2.0E-14 score: 63.9
IPR006593	Cytochrome b561/ferric reductase transmembrane	PFAM	PF03188	Cytochrom_B561	coord: 60..188 e-value: 3.6E-6 score: 27.2
IPR006593	Cytochrome b561/ferric reductase transmembrane	PROSITE	PS50939	CYTOCHROME_B561	coord: 27..221 score: 14.91152
None	No IPR available	GENE3D	1.20.120.1770		coord: 6..225 e-value: 7.8E-19 score: 69.7
None	No IPR available	PANTHER	PTHR23130	CYTOCHROME B561 AND DOMON DOMAIN-CONTAINING PROTEIN	coord: 19..236
None	No IPR available	PANTHER	PTHR23130:SF153	FAMILY PROTEIN, PUTATIVE-RELATED	coord: 19..236
None	No IPR available	CDD	cd08760	Cyt_b561_FRRS1_like	coord: 36..214 e-value: 5.94382E-20 score: 82.3926

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Carg13682-RA	Carg13682-RA	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
cellular_component	GO:0016021	integral component of membrane
molecular_function	GO:0016829	lyase activity
molecular_function	GO:0030170	pyridoxal phosphate binding