Carg13635 (gene) Silver-seed gourd (SMH-JMG-627) v2

Overview
NameCarg13635
Typegene
OrganismCucurbita argyrosperma subsp. argyrosperma cv. SMH-JMG-627 (Silver-seed gourd (SMH-JMG-627) v2)
DescriptionDNA-directed RNA polymerase III subunit rpc6
LocationCarg_Chr02: 286773 .. 288530 (-)
RNA-Seq ExpressionCarg13635
SyntenyCarg13635
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRCDSpolypeptidethree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
TTAGGCTTCACTCCATCGCCCTTGTACGTTTTAGAGAAACTGAACGCCCCCTCGGGATCGATTTGCCGATCAAAACTAATATCGCCGCCGCTCGTCATCCCACCTCTCCCCGCATCGTCTTCTCTCCGTCGCCTCTCGGGTTGGTTGTCTCTAGATTGCTTAAACCCTAGCTCTTTAGGGCGCTTCTCCATCGCTTTTGCGTTGCCACTGTTTCTGCAATTATCGTCTCTAAAATCTTGTAGTTCTGATATTTGATTTGAAGTCGCAGACTTTCAGGTATTTTTCCTGATTTCCTATGAAATTGCTTGAAGATCGATTCTTATATTATTTTATTTACTCTTCATTATGAATATATAAATGATTTACGCGCATATATATCATCTTGTTTTTGGTTTTTCTGATAGTTCCTAGCAACCATTTTCTTAACGTCTCTGCGAGTGGCTTGTTCTGCTAAGTTTACATATGATCGCTTCTCAATTTCTCACTTTTAGGTGCACGCGAAAGAAGAAAAATTGTGATTCTTCATTACCTATACTGCAGACCAAACTCAACGTGGTTTTTTCATGAATTTTTTTGGGATAATTGTTCATGGTGACACATATCATCCAGAACTCACAACAAAAATCTTTATGGATTTGAAATTGTTTTCCTCAAATTCTATAGTCTTTGAAACAGCATGATTATTTATTTATTTTTATTTTTTTTGTGCCTATGAATTGTTGAATATAGTGCTTCTTTGTTATTTGTTCAGTCATCCCATTTCCAAGGCACAGTTGTTCTCTCAGCTCAGCTCAGCTCAGCATCATTTTCAATTTCCTTAATCACATCTCTTACAAATCTTCACGTTTCCACATCATTTTGTTTTTATCAATCTCATTATGCACATATCATTTAATGTTAATAGGAATGAGCAGATCACAAGGGCCTTCATCACTCAAAAGAAAAAGGCCAGAACCAGCTCATGGCCATGGAGGAGCTCTCATTGAGCATGAAAAAGTGCTGTATGACCTGATTTGCAGCAAGCAAAGCATGGGGATTTGGATTGGGGACATGAAAAGAGAAACAAAGTATCCTGACAATGTGGTAACTAAATCACTCAAGGCCCTTCAAGGCAGAGGCCTGATTAAACTAGTTGAACACTATCAAAACAAAGGGAAGAAATTCTACCTGTCAAAAGATTTCGAACCATCGAAAGAACTGACCGGCGGTAATTGGTACAGCGATGGAAAGCTCGACAGAGAATACATCAGTACTCTTAAAGGGTTCTGTGCTAGAATGATACAGAAGATGAAGGTGGCTACAATTGAAGGGATCGTGGACTCATTCAAGAAGAGCGGAGCGTCCAAAGTGGACTTGTCTAGGGAGCAAATTGAAGAGATTGTAAATGCTTTGGTGTTGGACAATGAAGTGATGGAGGTGAAGAGCACTGGCGCAGGGGAGTTTGATTTTATTCCTGTTGGGAAGATTTGTTATAAGAGCTATGCTAAAGGAGTTAAAGGGGAGCCCAAAACAGGGGCTATGGCTTCTACTCCTTGTGGAGTCTGTCCAAGGATTAATCATTGCACCCCAAATGGCATCATTTCGCCTAGGACTTGTGTGTATTACACCAAGTGGTTAGAATTTTAGGTTTGAAGCTTTCTGAGGTTGGTTTTAAGAGAACGAGTGGCCGCGAATTTCCCCTCTAACCATACGAACTATGCTCGACTTGGCAATTCTAGAAAAGTTTTAGCTGTCTATTCTTTATTGTGTTCATAATCT

mRNA sequence

TTAGGCTTCACTCCATCGCCCTTGTACGTTTTAGAGAAACTGAACGCCCCCTCGGGATCGATTTGCCGATCAAAACTAATATCGCCGCCGCTCGTCATCCCACCTCTCCCCGCATCGTCTTCTCTCCGTCGCCTCTCGGGTTGGTTGTCTCTAGATTGCTTAAACCCTAGCTCTTTAGGGCGCTTCTCCATCGCTTTTGCGTTGCCACTGTTTCTGCAATTATCGTCTCTAAAATCTTGTAGTTCTGATATTTGATTTGAAGTCGCAGACTTTCAGATGAGCAGATCACAAGGGCCTTCATCACTCAAAAGAAAAAGGCCAGAACCAGCTCATGGCCATGGAGGAGCTCTCATTGAGCATGAAAAAGTGCTGTATGACCTGATTTGCAGCAAGCAAAGCATGGGGATTTGGATTGGGGACATGAAAAGAGAAACAAAGTATCCTGACAATGTGGTAACTAAATCACTCAAGGCCCTTCAAGGCAGAGGCCTGATTAAACTAGTTGAACACTATCAAAACAAAGGGAAGAAATTCTACCTGTCAAAAGATTTCGAACCATCGAAAGAACTGACCGGCGGTAATTGGTACAGCGATGGAAAGCTCGACAGAGAATACATCAGTACTCTTAAAGGGTTCTGTGCTAGAATGATACAGAAGATGAAGGTGGCTACAATTGAAGGGATCGTGGACTCATTCAAGAAGAGCGGAGCGTCCAAAGTGGACTTGTCTAGGGAGCAAATTGAAGAGATTGTAAATGCTTTGGTGTTGGACAATGAAGTGATGGAGGTGAAGAGCACTGGCGCAGGGGAGTTTGATTTTATTCCTGTTGGGAAGATTTGTTATAAGAGCTATGCTAAAGGAGTTAAAGGGGAGCCCAAAACAGGGGCTATGGCTTCTACTCCTTGTGGAGTCTGTCCAAGGATTAATCATTGCACCCCAAATGGCATCATTTCGCCTAGGACTTGTGTGTATTACACCAAGTGGTTAGAATTTTAGGTTTGAAGCTTTCTGAGGTTGGTTTTAAGAGAACGAGTGGCCGCGAATTTCCCCTCTAACCATACGAACTATGCTCGACTTGGCAATTCTAGAAAAGTTTTAGCTGTCTATTCTTTATTGTGTTCATAATCT

Coding sequence (CDS)

ATGAGCAGATCACAAGGGCCTTCATCACTCAAAAGAAAAAGGCCAGAACCAGCTCATGGCCATGGAGGAGCTCTCATTGAGCATGAAAAAGTGCTGTATGACCTGATTTGCAGCAAGCAAAGCATGGGGATTTGGATTGGGGACATGAAAAGAGAAACAAAGTATCCTGACAATGTGGTAACTAAATCACTCAAGGCCCTTCAAGGCAGAGGCCTGATTAAACTAGTTGAACACTATCAAAACAAAGGGAAGAAATTCTACCTGTCAAAAGATTTCGAACCATCGAAAGAACTGACCGGCGGTAATTGGTACAGCGATGGAAAGCTCGACAGAGAATACATCAGTACTCTTAAAGGGTTCTGTGCTAGAATGATACAGAAGATGAAGGTGGCTACAATTGAAGGGATCGTGGACTCATTCAAGAAGAGCGGAGCGTCCAAAGTGGACTTGTCTAGGGAGCAAATTGAAGAGATTGTAAATGCTTTGGTGTTGGACAATGAAGTGATGGAGGTGAAGAGCACTGGCGCAGGGGAGTTTGATTTTATTCCTGTTGGGAAGATTTGTTATAAGAGCTATGCTAAAGGAGTTAAAGGGGAGCCCAAAACAGGGGCTATGGCTTCTACTCCTTGTGGAGTCTGTCCAAGGATTAATCATTGCACCCCAAATGGCATCATTTCGCCTAGGACTTGTGTGTATTACACCAAGTGGTTAGAATTTTAG

Protein sequence

MSRSQGPSSLKRKRPEPAHGHGGALIEHEKVLYDLICSKQSMGIWIGDMKRETKYPDNVVTKSLKALQGRGLIKLVEHYQNKGKKFYLSKDFEPSKELTGGNWYSDGKLDREYISTLKGFCARMIQKMKVATIEGIVDSFKKSGASKVDLSREQIEEIVNALVLDNEVMEVKSTGAGEFDFIPVGKICYKSYAKGVKGEPKTGAMASTPCGVCPRINHCTPNGIISPRTCVYYTKWLEF
Homology
BLAST of Carg13635 vs. NCBI nr
Match: KAG6604735.1 (DNA-directed RNA polymerase III subunit rpc6, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 494.2 bits (1271), Expect = 6.3e-136
Identity = 239/239 (100.00%), Postives = 239/239 (100.00%), Query Frame = 0

Query: 1   MSRSQGPSSLKRKRPEPAHGHGGALIEHEKVLYDLICSKQSMGIWIGDMKRETKYPDNVV 60
           MSRSQGPSSLKRKRPEPAHGHGGALIEHEKVLYDLICSKQSMGIWIGDMKRETKYPDNVV
Sbjct: 89  MSRSQGPSSLKRKRPEPAHGHGGALIEHEKVLYDLICSKQSMGIWIGDMKRETKYPDNVV 148

Query: 61  TKSLKALQGRGLIKLVEHYQNKGKKFYLSKDFEPSKELTGGNWYSDGKLDREYISTLKGF 120
           TKSLKALQGRGLIKLVEHYQNKGKKFYLSKDFEPSKELTGGNWYSDGKLDREYISTLKGF
Sbjct: 149 TKSLKALQGRGLIKLVEHYQNKGKKFYLSKDFEPSKELTGGNWYSDGKLDREYISTLKGF 208

Query: 121 CARMIQKMKVATIEGIVDSFKKSGASKVDLSREQIEEIVNALVLDNEVMEVKSTGAGEFD 180
           CARMIQKMKVATIEGIVDSFKKSGASKVDLSREQIEEIVNALVLDNEVMEVKSTGAGEFD
Sbjct: 209 CARMIQKMKVATIEGIVDSFKKSGASKVDLSREQIEEIVNALVLDNEVMEVKSTGAGEFD 268

Query: 181 FIPVGKICYKSYAKGVKGEPKTGAMASTPCGVCPRINHCTPNGIISPRTCVYYTKWLEF 240
           FIPVGKICYKSYAKGVKGEPKTGAMASTPCGVCPRINHCTPNGIISPRTCVYYTKWLEF
Sbjct: 269 FIPVGKICYKSYAKGVKGEPKTGAMASTPCGVCPRINHCTPNGIISPRTCVYYTKWLEF 327

BLAST of Carg13635 vs. NCBI nr
Match: XP_022946967.1 (DNA-directed RNA polymerase III subunit rpc6 [Cucurbita moschata] >XP_023532566.1 DNA-directed RNA polymerase III subunit rpc6 [Cucurbita pepo subsp. pepo] >KAG7034865.1 DNA-directed RNA polymerase III subunit rpc6 [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 494.2 bits (1271), Expect = 6.3e-136
Identity = 239/239 (100.00%), Postives = 239/239 (100.00%), Query Frame = 0

Query: 1   MSRSQGPSSLKRKRPEPAHGHGGALIEHEKVLYDLICSKQSMGIWIGDMKRETKYPDNVV 60
           MSRSQGPSSLKRKRPEPAHGHGGALIEHEKVLYDLICSKQSMGIWIGDMKRETKYPDNVV
Sbjct: 1   MSRSQGPSSLKRKRPEPAHGHGGALIEHEKVLYDLICSKQSMGIWIGDMKRETKYPDNVV 60

Query: 61  TKSLKALQGRGLIKLVEHYQNKGKKFYLSKDFEPSKELTGGNWYSDGKLDREYISTLKGF 120
           TKSLKALQGRGLIKLVEHYQNKGKKFYLSKDFEPSKELTGGNWYSDGKLDREYISTLKGF
Sbjct: 61  TKSLKALQGRGLIKLVEHYQNKGKKFYLSKDFEPSKELTGGNWYSDGKLDREYISTLKGF 120

Query: 121 CARMIQKMKVATIEGIVDSFKKSGASKVDLSREQIEEIVNALVLDNEVMEVKSTGAGEFD 180
           CARMIQKMKVATIEGIVDSFKKSGASKVDLSREQIEEIVNALVLDNEVMEVKSTGAGEFD
Sbjct: 121 CARMIQKMKVATIEGIVDSFKKSGASKVDLSREQIEEIVNALVLDNEVMEVKSTGAGEFD 180

Query: 181 FIPVGKICYKSYAKGVKGEPKTGAMASTPCGVCPRINHCTPNGIISPRTCVYYTKWLEF 240
           FIPVGKICYKSYAKGVKGEPKTGAMASTPCGVCPRINHCTPNGIISPRTCVYYTKWLEF
Sbjct: 181 FIPVGKICYKSYAKGVKGEPKTGAMASTPCGVCPRINHCTPNGIISPRTCVYYTKWLEF 239

BLAST of Carg13635 vs. NCBI nr
Match: XP_022970903.1 (DNA-directed RNA polymerase III subunit rpc6 [Cucurbita maxima])

HSP 1 Score: 490.7 bits (1262), Expect = 7.0e-135
Identity = 237/239 (99.16%), Postives = 238/239 (99.58%), Query Frame = 0

Query: 1   MSRSQGPSSLKRKRPEPAHGHGGALIEHEKVLYDLICSKQSMGIWIGDMKRETKYPDNVV 60
           MSRSQGPSSLKRKRPEPAHGHGGALIEHEKVLYDLICSKQSMGIWIGDMKRETKYPDNVV
Sbjct: 1   MSRSQGPSSLKRKRPEPAHGHGGALIEHEKVLYDLICSKQSMGIWIGDMKRETKYPDNVV 60

Query: 61  TKSLKALQGRGLIKLVEHYQNKGKKFYLSKDFEPSKELTGGNWYSDGKLDREYISTLKGF 120
           TKSLKALQ RGLIKLVEHYQNKGKKFYLSKDFEPSKELTGGNWYSDGKLDREYISTLKGF
Sbjct: 61  TKSLKALQARGLIKLVEHYQNKGKKFYLSKDFEPSKELTGGNWYSDGKLDREYISTLKGF 120

Query: 121 CARMIQKMKVATIEGIVDSFKKSGASKVDLSREQIEEIVNALVLDNEVMEVKSTGAGEFD 180
           CARMIQKMKVATIEGIVD+FKKSGASKVDLSREQIEEIVNALVLDNEVMEVKSTGAGEFD
Sbjct: 121 CARMIQKMKVATIEGIVDAFKKSGASKVDLSREQIEEIVNALVLDNEVMEVKSTGAGEFD 180

Query: 181 FIPVGKICYKSYAKGVKGEPKTGAMASTPCGVCPRINHCTPNGIISPRTCVYYTKWLEF 240
           FIPVGKICYKSYAKGVKGEPKTGAMASTPCGVCPRINHCTPNGIISPRTCVYYTKWLEF
Sbjct: 181 FIPVGKICYKSYAKGVKGEPKTGAMASTPCGVCPRINHCTPNGIISPRTCVYYTKWLEF 239

BLAST of Carg13635 vs. NCBI nr
Match: XP_022149571.1 (DNA-directed RNA polymerase III subunit rpc6 isoform X1 [Momordica charantia] >XP_022149572.1 DNA-directed RNA polymerase III subunit rpc6 isoform X1 [Momordica charantia])

HSP 1 Score: 444.5 bits (1142), Expect = 5.8e-121
Identity = 215/240 (89.58%), Postives = 230/240 (95.83%), Query Frame = 0

Query: 1   MSRSQGPSSLKRKRPEPAHGHGGALIEHEKVLYDLICSKQSMGIWIGDMKRETKYPDNVV 60
           MSRSQGPSSLKRKRP+ +   GGALIEHEKVLYDLICSKQSMGIWIGD+KRETKY D++V
Sbjct: 1   MSRSQGPSSLKRKRPDSS--SGGALIEHEKVLYDLICSKQSMGIWIGDIKRETKYADSIV 60

Query: 61  TKSLKALQGRGLIKLVEHYQNKGKKFYLSKDFEPSKELTGGNWYSDGKLDREYISTLKGF 120
           TKSLKALQ +G+IKLVEHYQNKGKKFYLSKDFEPSKELTGGNWYS+GKLDREYI+TLKGF
Sbjct: 61  TKSLKALQAKGMIKLVEHYQNKGKKFYLSKDFEPSKELTGGNWYSEGKLDREYINTLKGF 120

Query: 121 CARMIQKMKVATIEGIVDSFKKSGASKVDLSREQIEEIVNALVLDNEVMEVKSTGAGEFD 180
           C RMIQKMKVATIEGIVDSFKKSGASKVDLSREQI+EIVNALVLDNEVMEVKSTG+GEFD
Sbjct: 121 CGRMIQKMKVATIEGIVDSFKKSGASKVDLSREQIQEIVNALVLDNEVMEVKSTGSGEFD 180

Query: 181 FIPVGKICYKSYAK-GVKGEPKTGAMASTPCGVCPRINHCTPNGIISPRTCVYYTKWLEF 240
           FIP+GKICYK YAK GVKGEPKTGAMAS PCGVCPRI+HCTPNG+ISPRTCVYYTKWL+F
Sbjct: 181 FIPIGKICYKCYAKGGVKGEPKTGAMASIPCGVCPRISHCTPNGVISPRTCVYYTKWLDF 238

BLAST of Carg13635 vs. NCBI nr
Match: XP_038900531.1 (LOW QUALITY PROTEIN: DNA-directed RNA polymerase III subunit RPC6-like [Benincasa hispida])

HSP 1 Score: 409.5 bits (1051), Expect = 2.1e-110
Identity = 200/238 (84.03%), Postives = 217/238 (91.18%), Query Frame = 0

Query: 1   MSRSQGPSSLKRKRPEPAHGHGGALIEHEKVLYDLICSKQSMGIWIGDMKRETKYPDNVV 60
           MS+ QGPSSLKRKR + AHG GGALIEHEKVL+DLICSKQ+MGIWIGD+++ETKY D VV
Sbjct: 1   MSKPQGPSSLKRKRLDSAHGFGGALIEHEKVLHDLICSKQNMGIWIGDIRKETKYADGVV 60

Query: 61  TKSLKALQGRGLIKLVEHYQNKGKKFYLSKDFEPSKELTGGNWYSDGKLDREYISTLKGF 120
            K LK+LQ RGLIKLVEHYQNKGKKFYLSKDFEPSKELTGGNWYSDGKLD+EYI+TLKGF
Sbjct: 61  RKLLKSLQARGLIKLVEHYQNKGKKFYLSKDFEPSKELTGGNWYSDGKLDQEYINTLKGF 120

Query: 121 CARMIQKMKVATIEGIVDSFKKSGASKVDLSREQIEEIVNALVLDNEVMEVKSTGAGEFD 180
           CARMIQK+KV+TIEG V SFKKSG SKVDLSREQIEEIVNALVLDNEVMEVK TG+ EFD
Sbjct: 121 CARMIQKLKVSTIEGTVXSFKKSGTSKVDLSREQIEEIVNALVLDNEVMEVKRTGSREFD 180

Query: 181 FIPVGKICYKSYAKGVKGEPKTGAMASTPCGVCPRINHCTPNGIISPRTCVYYTK-WL 238
           FIPV KICYK YAK VKGEPKTGAMAS PCGVCPRI+HCT +GIIS RTC+YYTK W+
Sbjct: 181 FIPVRKICYKCYAKDVKGEPKTGAMASIPCGVCPRISHCTLDGIISHRTCMYYTKCWI 238

BLAST of Carg13635 vs. ExPASy Swiss-Prot
Match: Q86JM3 (DNA-directed RNA polymerase III subunit rpc6 OS=Dictyostelium discoideum OX=44689 GN=polr3f PE=3 SV=1)

HSP 1 Score: 122.9 bits (307), Expect = 5.0e-27
Identity = 73/240 (30.42%), Postives = 118/240 (49.17%), Query Frame = 0

Query: 1   MSRSQGPSSLKRKRPEPAHGHGGALIEHEKVLYDLICSKQSMGIWIGDMKRETKYPDNVV 60
           +  S   +S K   PE      G  +E + ++Y LI +  + G W  ++K  + Y    +
Sbjct: 52  IQNSDETTSYKEVNPEDQAKFRGLGVE-DFLIYQLIEASSNTGAWTRELKNSSGYQQVQI 111

Query: 61  TKSLKALQGRGLIKLVEHYQNKGKKFYLSKDFEPSKELTGGNWYSDGKLDREYISTLKGF 120
           TK LK L+ R LIK V+  Q+  KK Y+  + EPS+E+TGG  YSD   D +YI  +K  
Sbjct: 112 TKILKTLESRKLIKSVKSIQSGRKKVYMLYNMEPSREITGGQLYSDQSYDHQYIQIMKMH 171

Query: 121 CARMIQKMKVATIEGIVDSFKK--SGASKVDLSREQIEEIVNALVLDNEVMEVKSTGAGE 180
               +       +  I+   +K    A+   L  E I+ +VN ++ D ++ E++ T  G 
Sbjct: 172 IKTFVDNKGAVDLADIITYLRKVAEEATSQSLGPEDIQALVNTVIYDGDIEEMRDTRMGA 231

Query: 181 FDFIPVGKICYKSYAKGVKGEPKTGAMASTPCGVCPRINHCTPNGIISPRTCVYYTKWLE 239
                +G+     Y K  K         + PCG CP  + C+ NG++SP+ CVY+ ++LE
Sbjct: 232 M----LGRRSGILY-KPTKTPIPVNNFCNMPCGNCPVFDICSDNGLVSPKRCVYFKQYLE 285

BLAST of Carg13635 vs. ExPASy Swiss-Prot
Match: Q2T9S3 (DNA-directed RNA polymerase III subunit RPC6 OS=Bos taurus OX=9913 GN=POLR3F PE=2 SV=1)

HSP 1 Score: 120.6 bits (301), Expect = 2.5e-26
Identity = 73/229 (31.88%), Postives = 112/229 (48.91%), Query Frame = 0

Query: 29  EKVLYDLICSKQSMGIWIGDMKRETKYPDNVVTKSLKALQGRGLIKLVEHYQNKGKKFYL 88
           EK++Y +I    + GIW  D++ ++  P   + K LK L+ + LIK V+      KK Y+
Sbjct: 92  EKLVYQIIEDAGNKGIWSRDIRYKSNLPLTEINKILKNLESKKLIKAVKSVAASKKKVYM 151

Query: 89  SKDFEPSKELTGGNWYSDGKLDREYISTLKGFCARMIQKMKVATIEGIVD-------SFK 148
             + +P + +TGG WYSD   + E++  L   C + +Q       E   +       SF 
Sbjct: 152 LYNLQPDRSVTGGAWYSDQDFESEFVEVLNQQCFKFLQNKAETARESKQNPMIQRNSSFA 211

Query: 149 KS----------GASKVDLSREQIEEIVNALVLDNEV-MEVKSTGAGEFDFIPVGKICYK 208
            S          G SKV+LS E IE I+N L+ D +V M + +   G    +      Y+
Sbjct: 212 SSHEVWKYICELGISKVELSMEDIETILNTLIYDGKVEMTIIAAKEGTVGSVDGHMKLYR 271

Query: 209 SYAKGVKGEPKTGAMASTPCGVCPRINHCTPNGIISPRTCVYYTKWLEF 240
           + +  +   P TG +   PCG+CP  + C   G ISP  C+Y T+WLEF
Sbjct: 272 AVSPII---PPTG-LVRAPCGLCPVFDDCHEGGEISPSNCIYMTEWLEF 316

BLAST of Carg13635 vs. ExPASy Swiss-Prot
Match: Q9H1D9 (DNA-directed RNA polymerase III subunit RPC6 OS=Homo sapiens OX=9606 GN=POLR3F PE=1 SV=1)

HSP 1 Score: 119.4 bits (298), Expect = 5.6e-26
Identity = 73/229 (31.88%), Postives = 111/229 (48.47%), Query Frame = 0

Query: 29  EKVLYDLICSKQSMGIWIGDMKRETKYPDNVVTKSLKALQGRGLIKLVEHYQNKGKKFYL 88
           EK++Y +I    + GIW  D++ ++  P   + K LK L+ + LIK V+      KK Y+
Sbjct: 92  EKLVYQIIEDAGNKGIWSRDIRYKSNLPLTEINKILKNLESKKLIKAVKSVAASKKKVYM 151

Query: 89  SKDFEPSKELTGGNWYSDGKLDREYISTLKGFCARMIQKMKVATIEGIVD-------SFK 148
             + +P + +TGG WYSD   + E++  L   C + +Q       E   +       SF 
Sbjct: 152 LYNLQPDRSVTGGAWYSDQDFESEFVEVLNQQCFKFLQSKAETARESKQNPMIQRNSSFA 211

Query: 149 KS----------GASKVDLSREQIEEIVNALVLDNEV-MEVKSTGAGEFDFIPVGKICYK 208
            S          G SKV+LS E IE I+N L+ D +V M + +   G    +      Y+
Sbjct: 212 SSHEVWKYICELGISKVELSMEDIETILNTLIYDGKVEMTIIAAKEGTVGSVDGHMKLYR 271

Query: 209 SYAKGVKGEPKTGAMASTPCGVCPRINHCTPNGIISPRTCVYYTKWLEF 240
           +    +   P TG +   PCG+CP  + C   G ISP  C+Y T+WLEF
Sbjct: 272 AVNPII---PPTG-LVRAPCGLCPVFDDCHEGGEISPSNCIYMTEWLEF 316

BLAST of Carg13635 vs. ExPASy Swiss-Prot
Match: Q921X6 (DNA-directed RNA polymerase III subunit RPC6 OS=Mus musculus OX=10090 GN=Polr3f PE=1 SV=1)

HSP 1 Score: 118.2 bits (295), Expect = 1.2e-25
Identity = 73/229 (31.88%), Postives = 111/229 (48.47%), Query Frame = 0

Query: 29  EKVLYDLICSKQSMGIWIGDMKRETKYPDNVVTKSLKALQGRGLIKLVEHYQNKGKKFYL 88
           EK++Y +I    + GIW  D++ ++  P   + K LK L+ + LIK V+      KK Y+
Sbjct: 92  EKLVYQIIEDAGNKGIWSRDIRYKSNLPLTEINKILKNLESKKLIKAVKSVAASKKKVYM 151

Query: 89  SKDFEPSKELTGGNWYSDGKLDREYISTLKGFCARMIQKMKVATIEGIVD-------SFK 148
             + +P + +TGG WYSD   + E++  L   C + +Q       E   +       SF 
Sbjct: 152 LYNLQPDRSVTGGAWYSDQDFESEFVEVLNQQCFKFLQSKAETARESKQNPVIQRNSSFA 211

Query: 149 KS----------GASKVDLSREQIEEIVNALVLDNEV-MEVKSTGAGEFDFIPVGKICYK 208
            S          G SKV+LS E IE I+N L+ D +V M + +   G    +      Y+
Sbjct: 212 SSHEVWKYICELGISKVELSMEDIETILNTLIYDGKVEMTIIAAKEGTVGSVDGHMKLYR 271

Query: 209 SYAKGVKGEPKTGAMASTPCGVCPRINHCTPNGIISPRTCVYYTKWLEF 240
           +    +   P TG + + PCG+CP    C   G ISP  C+Y T+WLEF
Sbjct: 272 AVNPIL---PPTGVVRA-PCGLCPVFEDCHEGGEISPSNCIYMTEWLEF 316

BLAST of Carg13635 vs. ExPASy Swiss-Prot
Match: Q9VD25 (Probable DNA-directed RNA polymerase III subunit RPC6 OS=Drosophila melanogaster OX=7227 GN=CG5380 PE=2 SV=1)

HSP 1 Score: 102.1 bits (253), Expect = 9.2e-21
Identity = 63/227 (27.75%), Postives = 101/227 (44.49%), Query Frame = 0

Query: 29  EKVLYDLICSKQSMGIWIGDMKRETKYPDNVVTKSLKALQGRGLIKLVEHYQNKGKKFYL 88
           EKV+Y ++    + GIWI D++ ++      + K LK L+ + LIK V+      KK Y+
Sbjct: 80  EKVVYGIVEEGGNKGIWIRDIRMKSNLNMIQLNKILKNLETKKLIKAVKSVNASKKKVYM 139

Query: 89  SKDFEPSKELTGGNWYSDGKLDREYISTLKGFCARMIQ-----------------KMKVA 148
             + EP   +TGG WY D   + E++  L   C R +Q                 +M   
Sbjct: 140 LYNLEPDLSITGGAWYQDQDFEVEFVDVLNQQCLRFLQMKRDSAEKKREGPLAFKQMSCC 199

Query: 149 TIEGIVDSFKKSGASKVDLSREQIEEIVNALVLDNEVMEVKSTGAGEFDFIPVGKICYKS 208
           T+  +       G SKV+L+   +E I+  +V D     V+            G   Y++
Sbjct: 200 TVNEVQKFISDLGISKVNLAEADLETILKTVVYDGNAERVRQQD---------GSFVYRA 259

Query: 209 YAKGVKGEPKTGAMASTPCGVCPRINHCTPNGIISPRTCVYYTKWLE 239
               +   P TG +   PCG+CP I +C+  G ++  TC Y   WL+
Sbjct: 260 VNAPL---PPTG-LVQMPCGICPVIKNCSNCGDVTAITCEYMRDWLD 293

BLAST of Carg13635 vs. ExPASy TrEMBL
Match: A0A6J1G553 (DNA-directed RNA polymerase III subunit rpc6 OS=Cucurbita moschata OX=3662 GN=LOC111450988 PE=3 SV=1)

HSP 1 Score: 494.2 bits (1271), Expect = 3.1e-136
Identity = 239/239 (100.00%), Postives = 239/239 (100.00%), Query Frame = 0

Query: 1   MSRSQGPSSLKRKRPEPAHGHGGALIEHEKVLYDLICSKQSMGIWIGDMKRETKYPDNVV 60
           MSRSQGPSSLKRKRPEPAHGHGGALIEHEKVLYDLICSKQSMGIWIGDMKRETKYPDNVV
Sbjct: 1   MSRSQGPSSLKRKRPEPAHGHGGALIEHEKVLYDLICSKQSMGIWIGDMKRETKYPDNVV 60

Query: 61  TKSLKALQGRGLIKLVEHYQNKGKKFYLSKDFEPSKELTGGNWYSDGKLDREYISTLKGF 120
           TKSLKALQGRGLIKLVEHYQNKGKKFYLSKDFEPSKELTGGNWYSDGKLDREYISTLKGF
Sbjct: 61  TKSLKALQGRGLIKLVEHYQNKGKKFYLSKDFEPSKELTGGNWYSDGKLDREYISTLKGF 120

Query: 121 CARMIQKMKVATIEGIVDSFKKSGASKVDLSREQIEEIVNALVLDNEVMEVKSTGAGEFD 180
           CARMIQKMKVATIEGIVDSFKKSGASKVDLSREQIEEIVNALVLDNEVMEVKSTGAGEFD
Sbjct: 121 CARMIQKMKVATIEGIVDSFKKSGASKVDLSREQIEEIVNALVLDNEVMEVKSTGAGEFD 180

Query: 181 FIPVGKICYKSYAKGVKGEPKTGAMASTPCGVCPRINHCTPNGIISPRTCVYYTKWLEF 240
           FIPVGKICYKSYAKGVKGEPKTGAMASTPCGVCPRINHCTPNGIISPRTCVYYTKWLEF
Sbjct: 181 FIPVGKICYKSYAKGVKGEPKTGAMASTPCGVCPRINHCTPNGIISPRTCVYYTKWLEF 239

BLAST of Carg13635 vs. ExPASy TrEMBL
Match: A0A6J1I599 (DNA-directed RNA polymerase III subunit rpc6 OS=Cucurbita maxima OX=3661 GN=LOC111469737 PE=3 SV=1)

HSP 1 Score: 490.7 bits (1262), Expect = 3.4e-135
Identity = 237/239 (99.16%), Postives = 238/239 (99.58%), Query Frame = 0

Query: 1   MSRSQGPSSLKRKRPEPAHGHGGALIEHEKVLYDLICSKQSMGIWIGDMKRETKYPDNVV 60
           MSRSQGPSSLKRKRPEPAHGHGGALIEHEKVLYDLICSKQSMGIWIGDMKRETKYPDNVV
Sbjct: 1   MSRSQGPSSLKRKRPEPAHGHGGALIEHEKVLYDLICSKQSMGIWIGDMKRETKYPDNVV 60

Query: 61  TKSLKALQGRGLIKLVEHYQNKGKKFYLSKDFEPSKELTGGNWYSDGKLDREYISTLKGF 120
           TKSLKALQ RGLIKLVEHYQNKGKKFYLSKDFEPSKELTGGNWYSDGKLDREYISTLKGF
Sbjct: 61  TKSLKALQARGLIKLVEHYQNKGKKFYLSKDFEPSKELTGGNWYSDGKLDREYISTLKGF 120

Query: 121 CARMIQKMKVATIEGIVDSFKKSGASKVDLSREQIEEIVNALVLDNEVMEVKSTGAGEFD 180
           CARMIQKMKVATIEGIVD+FKKSGASKVDLSREQIEEIVNALVLDNEVMEVKSTGAGEFD
Sbjct: 121 CARMIQKMKVATIEGIVDAFKKSGASKVDLSREQIEEIVNALVLDNEVMEVKSTGAGEFD 180

Query: 181 FIPVGKICYKSYAKGVKGEPKTGAMASTPCGVCPRINHCTPNGIISPRTCVYYTKWLEF 240
           FIPVGKICYKSYAKGVKGEPKTGAMASTPCGVCPRINHCTPNGIISPRTCVYYTKWLEF
Sbjct: 181 FIPVGKICYKSYAKGVKGEPKTGAMASTPCGVCPRINHCTPNGIISPRTCVYYTKWLEF 239

BLAST of Carg13635 vs. ExPASy TrEMBL
Match: A0A6J1D8D1 (DNA-directed RNA polymerase III subunit rpc6 isoform X1 OS=Momordica charantia OX=3673 GN=LOC111017972 PE=3 SV=1)

HSP 1 Score: 444.5 bits (1142), Expect = 2.8e-121
Identity = 215/240 (89.58%), Postives = 230/240 (95.83%), Query Frame = 0

Query: 1   MSRSQGPSSLKRKRPEPAHGHGGALIEHEKVLYDLICSKQSMGIWIGDMKRETKYPDNVV 60
           MSRSQGPSSLKRKRP+ +   GGALIEHEKVLYDLICSKQSMGIWIGD+KRETKY D++V
Sbjct: 1   MSRSQGPSSLKRKRPDSS--SGGALIEHEKVLYDLICSKQSMGIWIGDIKRETKYADSIV 60

Query: 61  TKSLKALQGRGLIKLVEHYQNKGKKFYLSKDFEPSKELTGGNWYSDGKLDREYISTLKGF 120
           TKSLKALQ +G+IKLVEHYQNKGKKFYLSKDFEPSKELTGGNWYS+GKLDREYI+TLKGF
Sbjct: 61  TKSLKALQAKGMIKLVEHYQNKGKKFYLSKDFEPSKELTGGNWYSEGKLDREYINTLKGF 120

Query: 121 CARMIQKMKVATIEGIVDSFKKSGASKVDLSREQIEEIVNALVLDNEVMEVKSTGAGEFD 180
           C RMIQKMKVATIEGIVDSFKKSGASKVDLSREQI+EIVNALVLDNEVMEVKSTG+GEFD
Sbjct: 121 CGRMIQKMKVATIEGIVDSFKKSGASKVDLSREQIQEIVNALVLDNEVMEVKSTGSGEFD 180

Query: 181 FIPVGKICYKSYAK-GVKGEPKTGAMASTPCGVCPRINHCTPNGIISPRTCVYYTKWLEF 240
           FIP+GKICYK YAK GVKGEPKTGAMAS PCGVCPRI+HCTPNG+ISPRTCVYYTKWL+F
Sbjct: 181 FIPIGKICYKCYAKGGVKGEPKTGAMASIPCGVCPRISHCTPNGVISPRTCVYYTKWLDF 238

BLAST of Carg13635 vs. ExPASy TrEMBL
Match: A0A6J1D758 (DNA-directed RNA polymerase III subunit rpc6 isoform X2 OS=Momordica charantia OX=3673 GN=LOC111017972 PE=3 SV=1)

HSP 1 Score: 380.6 bits (976), Expect = 4.9e-102
Identity = 180/199 (90.45%), Postives = 193/199 (96.98%), Query Frame = 0

Query: 42  MGIWIGDMKRETKYPDNVVTKSLKALQGRGLIKLVEHYQNKGKKFYLSKDFEPSKELTGG 101
           MGIWIGD+KRETKY D++VTKSLKALQ +G+IKLVEHYQNKGKKFYLSKDFEPSKELTGG
Sbjct: 1   MGIWIGDIKRETKYADSIVTKSLKALQAKGMIKLVEHYQNKGKKFYLSKDFEPSKELTGG 60

Query: 102 NWYSDGKLDREYISTLKGFCARMIQKMKVATIEGIVDSFKKSGASKVDLSREQIEEIVNA 161
           NWYS+GKLDREYI+TLKGFC RMIQKMKVATIEGIVDSFKKSGASKVDLSREQI+EIVNA
Sbjct: 61  NWYSEGKLDREYINTLKGFCGRMIQKMKVATIEGIVDSFKKSGASKVDLSREQIQEIVNA 120

Query: 162 LVLDNEVMEVKSTGAGEFDFIPVGKICYKSYAK-GVKGEPKTGAMASTPCGVCPRINHCT 221
           LVLDNEVMEVKSTG+GEFDFIP+GKICYK YAK GVKGEPKTGAMAS PCGVCPRI+HCT
Sbjct: 121 LVLDNEVMEVKSTGSGEFDFIPIGKICYKCYAKGGVKGEPKTGAMASIPCGVCPRISHCT 180

Query: 222 PNGIISPRTCVYYTKWLEF 240
           PNG+ISPRTCVYYTKWL+F
Sbjct: 181 PNGVISPRTCVYYTKWLDF 199

BLAST of Carg13635 vs. ExPASy TrEMBL
Match: A0A1S3BL06 (DNA-directed RNA polymerase III subunit rpc6-like OS=Cucumis melo OX=3656 GN=LOC103490992 PE=3 SV=1)

HSP 1 Score: 378.3 bits (970), Expect = 2.5e-101
Identity = 185/243 (76.13%), Postives = 213/243 (87.65%), Query Frame = 0

Query: 1   MSRSQGPSSLKRKRPE--PAHGHGGALIEHEKVLYDLICSKQSMGIWIGDMKRETKYPDN 60
           MS+SQGPSSLKRKRP+    +  GGALI  EKVLYDLICSKQSMGI   DMKRETK+ ++
Sbjct: 1   MSKSQGPSSLKRKRPDLGTKNSSGGALIGQEKVLYDLICSKQSMGICTFDMKRETKFAES 60

Query: 61  VVTKSLKALQGRGLIKLVEHYQNKGKKFYLSKDFEPSKELTGGNWYSDGKLDREYISTLK 120
           VVTKSLK+LQ +GLIK+V+HYQNKGKKFYLS DFEP+KELTGGNWY DG+LD++YI+TLK
Sbjct: 61  VVTKSLKSLQTKGLIKIVQHYQNKGKKFYLSNDFEPAKELTGGNWYRDGELDKDYINTLK 120

Query: 121 GFCARMIQKMKVATIEGIVDSFKKSGASKVDLSREQIEEIVNALVLDNEVMEVKSTGAGE 180
             CARMIQ+ K+ TIEGIV+SFK+SGA KV+LSREQIEEIVNALVLDN+VMEVKS+G GE
Sbjct: 121 QVCARMIQRKKLMTIEGIVESFKQSGAFKVELSREQIEEIVNALVLDNQVMEVKSSGFGE 180

Query: 181 FDFIPVGKICYK--SYAKGVKGEPKTGAMASTPCGVCPRINHCTPNGIISPRTCVYYTKW 240
           F+FIP+GKICYK  +   G+KGEPK GAMAS PCGVCPRI +CTPNGIISP TCVYYTKW
Sbjct: 181 FEFIPIGKICYKYCTQGGGLKGEPKIGAMASIPCGVCPRITYCTPNGIISPATCVYYTKW 240

BLAST of Carg13635 vs. TAIR 10
Match: AT5G23710.1 (DNA binding;DNA-directed RNA polymerases )

HSP 1 Score: 201.8 bits (512), Expect = 6.1e-52
Identity = 105/232 (45.26%), Postives = 142/232 (61.21%), Query Frame = 0

Query: 11  KRKRPEPAHGHGGALIEHEKVLYDLICSKQSMGIWIGDMKRETKYPDNVVTKSLKALQGR 70
           KRKRP+P    G     HEK L DLI   Q  G  + ++KRE   P  +VT+ + +L+ +
Sbjct: 3   KRKRPDPKSSGGDLNEPHEKKLLDLIRINQGRGATMFELKREKTIPATIVTRLIASLRKK 62

Query: 71  GLIKLVEHYQNKGKKFYLSKDFEPSKELTGGNWYSDGKLDREYISTLKGFCARMIQ--KM 130
            LIK V +  NKG K YL+ +FEP  ELTGG WY+DG LD   I  LK  C  +++  + 
Sbjct: 63  NLIKEVANMNNKGVKHYLAMEFEPCSELTGGEWYTDGALDLSKIEDLKAKCVMILERHRQ 122

Query: 131 KVATIEGIVDSFKKSGASKVDLSREQIEEIVNALVLDNEVMEVKSTGAGEFDFIPVGKIC 190
           +V T+E +   F K    +  LS +Q +EI+  L+LDN +MEVKS G  EF    +G++C
Sbjct: 123 RVVTLEVLCGYFVK----EEKLSVDQTKEILKNLILDNLIMEVKSNGMNEFASTRIGEVC 182

Query: 191 YKSYAKGV-KGEPKTGAMASTPCGVCPRINHCTPNGIISPRTCVYYTKWLEF 240
           Y+   K    GEP+ GA AS PCGVCP I  C+P+G+ISP TCVY+ KWL+F
Sbjct: 183 YRLTGKKFGNGEPRAGAFASIPCGVCPHIAICSPDGVISPTTCVYFQKWLDF 230

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
KAG6604735.16.3e-136100.00DNA-directed RNA polymerase III subunit rpc6, partial [Cucurbita argyrosperma su... [more]
XP_022946967.16.3e-136100.00DNA-directed RNA polymerase III subunit rpc6 [Cucurbita moschata] >XP_023532566.... [more]
XP_022970903.17.0e-13599.16DNA-directed RNA polymerase III subunit rpc6 [Cucurbita maxima][more]
XP_022149571.15.8e-12189.58DNA-directed RNA polymerase III subunit rpc6 isoform X1 [Momordica charantia] >X... [more]
XP_038900531.12.1e-11084.03LOW QUALITY PROTEIN: DNA-directed RNA polymerase III subunit RPC6-like [Benincas... [more]
Match NameE-valueIdentityDescription
Q86JM35.0e-2730.42DNA-directed RNA polymerase III subunit rpc6 OS=Dictyostelium discoideum OX=4468... [more]
Q2T9S32.5e-2631.88DNA-directed RNA polymerase III subunit RPC6 OS=Bos taurus OX=9913 GN=POLR3F PE=... [more]
Q9H1D95.6e-2631.88DNA-directed RNA polymerase III subunit RPC6 OS=Homo sapiens OX=9606 GN=POLR3F P... [more]
Q921X61.2e-2531.88DNA-directed RNA polymerase III subunit RPC6 OS=Mus musculus OX=10090 GN=Polr3f ... [more]
Q9VD259.2e-2127.75Probable DNA-directed RNA polymerase III subunit RPC6 OS=Drosophila melanogaster... [more]
Match NameE-valueIdentityDescription
A0A6J1G5533.1e-136100.00DNA-directed RNA polymerase III subunit rpc6 OS=Cucurbita moschata OX=3662 GN=LO... [more]
A0A6J1I5993.4e-13599.16DNA-directed RNA polymerase III subunit rpc6 OS=Cucurbita maxima OX=3661 GN=LOC1... [more]
A0A6J1D8D12.8e-12189.58DNA-directed RNA polymerase III subunit rpc6 isoform X1 OS=Momordica charantia O... [more]
A0A6J1D7584.9e-10290.45DNA-directed RNA polymerase III subunit rpc6 isoform X2 OS=Momordica charantia O... [more]
A0A1S3BL062.5e-10176.13DNA-directed RNA polymerase III subunit rpc6-like OS=Cucumis melo OX=3656 GN=LOC... [more]
Match NameE-valueIdentityDescription
AT5G23710.16.1e-5245.26DNA binding;DNA-directed RNA polymerases [more]
InterPro
Analysis Name: InterPro Annotations of Silver-seed gourd (SMH-JMG-627) v2
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR036388Winged helix-like DNA-binding domain superfamilyGENE3D1.10.10.10coord: 27..90
e-value: 1.3E-15
score: 59.1
IPR007832RNA polymerase Rpc34PFAMPF05158RNA_pol_Rpc34coord: 128..238
e-value: 1.1E-15
score: 57.9
coord: 26..127
e-value: 1.0E-24
score: 87.6
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 1..22
IPR016049RNA polymerase Rpc34-likePANTHERPTHR12780RNA POLYMERASE III DNA DIRECTED , 39KD SUBUNIT-RELATEDcoord: 13..238
IPR036390Winged helix DNA-binding domain superfamilySUPERFAMILY46785"Winged helix" DNA-binding domaincoord: 27..89

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Carg13635-RACarg13635-RAmRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0006383 transcription by RNA polymerase III
cellular_component GO:0005666 RNA polymerase III complex
molecular_function GO:0003899 DNA-directed 5'-3' RNA polymerase activity
molecular_function GO:0008168 methyltransferase activity