Carg13592 (gene) Silver-seed gourd (SMH-JMG-627) v2

BLAST of Carg13592 vs. NCBI nr
Match: KAG7034908.1 (putative E3 ubiquitin-protein ligase RING1a [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 990.7 bits (2560), Expect = 4.6e-285
Identity = 509/509 (100.00%), Postives = 509/509 (100.00%), Query Frame = 0

Query: 1   MPAHKRSWSDSLVGEELSDEQFSHQDAKQCRAERQPNTEVEEEGEEEQKKPKKEAEREEK 60
           MPAHKRSWSDSLVGEELSDEQFSHQDAKQCRAERQPNTEVEEEGEEEQKKPKKEAEREEK
Sbjct: 1   MPAHKRSWSDSLVGEELSDEQFSHQDAKQCRAERQPNTEVEEEGEEEQKKPKKEAEREEK 60

Query: 61  QHKQLEEGTGARDSEESQSSSSEEKPEYFLLNPSTSFSLYDCHFNGAVDLMCGKFIFVEL 120
           QHKQLEEGTGARDSEESQSSSSEEKPEYFLLNPSTSFSLYDCHFNGAVDLMCGKFIFVEL
Sbjct: 61  QHKQLEEGTGARDSEESQSSSSEEKPEYFLLNPSTSFSLYDCHFNGAVDLMCGKFIFVEL 120

Query: 121 SGIRKDVQCPICLGIIKKTRTVMECLHRFCRECIDKSMRLGNNECPACRTHCASRRSLRD 180
           SGIRKDVQCPICLGIIKKTRTVMECLHRFCRECIDKSMRLGNNECPACRTHCASRRSLRD
Sbjct: 121 SGIRKDVQCPICLGIIKKTRTVMECLHRFCRECIDKSMRLGNNECPACRTHCASRRSLRD 180

Query: 181 DPNYDALIAALYPDIDKYEEEELTFHEEERTRNKQIQESIAQTFQRQSEALSKKRMLGKD 240
           DPNYDALIAALYPDIDKYEEEELTFHEEERTRNKQIQESIAQTFQRQSEALSKKRMLGKD
Sbjct: 181 DPNYDALIAALYPDIDKYEEEELTFHEEERTRNKQIQESIAQTFQRQSEALSKKRMLGKD 240

Query: 241 MPGMILTRSRRNHRNVHLRRQNGRGDELLGYEDNEDEDDNSEGKDSSSADERCSKVRQRR 300
           MPGMILTRSRRNHRNVHLRRQNGRGDELLGYEDNEDEDDNSEGKDSSSADERCSKVRQRR
Sbjct: 241 MPGMILTRSRRNHRNVHLRRQNGRGDELLGYEDNEDEDDNSEGKDSSSADERCSKVRQRR 300

Query: 301 KKRHSTVRSFQPSSSIASVDYGCTDGCAESDLDMSRENRTVSPGLVLNSEMLGWGRGGVR 360
           KKRHSTVRSFQPSSSIASVDYGCTDGCAESDLDMSRENRTVSPGLVLNSEMLGWGRGGVR
Sbjct: 301 KKRHSTVRSFQPSSSIASVDYGCTDGCAESDLDMSRENRTVSPGLVLNSEMLGWGRGGVR 360

Query: 361 SNTRYGNAGGLSNKGSRSGRLMKLVNYLRGLEENNNELDVHILLISVDSESTPSLKQPHL 420
           SNTRYGNAGGLSNKGSRSGRLMKLVNYLRGLEENNNELDVHILLISVDSESTPSLKQPHL
Sbjct: 361 SNTRYGNAGGLSNKGSRSGRLMKLVNYLRGLEENNNELDVHILLISVDSESTPSLKQPHL 420

Query: 421 QCRPSLTVKHLREYVSRKTPLPADDVELLSVKDRYGTSDAQSTPSSSIFNPLKYELQILG 480
           QCRPSLTVKHLREYVSRKTPLPADDVELLSVKDRYGTSDAQSTPSSSIFNPLKYELQILG
Sbjct: 421 QCRPSLTVKHLREYVSRKTPLPADDVELLSVKDRYGTSDAQSTPSSSIFNPLKYELQILG 480

Query: 481 GEETLAGLQADCTYSREHLILGYRWKGRS 510
           GEETLAGLQADCTYSREHLILGYRWKGRS
Sbjct: 481 GEETLAGLQADCTYSREHLILGYRWKGRS 509

BLAST of Carg13592 vs. NCBI nr
Match: KAG6604785.1 (putative E3 ubiquitin-protein ligase RING1a, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 910.2 bits (2351), Expect = 7.9e-261
Identity = 480/509 (94.30%), Postives = 480/509 (94.30%), Query Frame = 0

Query: 1   MPAHKRSWSDSLVGEELSDEQFSHQDAKQCRAERQPNTEVEEEGEEEQKKPKKEAEREEK 60
           MPAHKRSWSDSLVGEELSDEQFSHQDAKQCRAERQPNTEVEEE EEEQKKPKKEAEREEK
Sbjct: 1   MPAHKRSWSDSLVGEELSDEQFSHQDAKQCRAERQPNTEVEEEEEEEQKKPKKEAEREEK 60

Query: 61  QHKQLEEGTGARDSEESQSSSSEEKPEYFLLNPSTSFSLYDCHFNGAVDLMCGKFIFVEL 120
           QHKQLEEGTGARDSEESQSSSSEEKPE                           FIFVEL
Sbjct: 61  QHKQLEEGTGARDSEESQSSSSEEKPE---------------------------FIFVEL 120

Query: 121 SGIRKDVQCPICLGIIKKTRTVMECLHRFCRECIDKSMRLGNNECPACRTHCASRRSLRD 180
           SGIRKDVQCPICLGIIKKTRTVMECLHRFCRECIDKSMRLGNNECPACRTHCASRRSLRD
Sbjct: 121 SGIRKDVQCPICLGIIKKTRTVMECLHRFCRECIDKSMRLGNNECPACRTHCASRRSLRD 180

Query: 181 DPNYDALIAALYPDIDKYEEEELTFHEEERTRNKQIQESIAQTFQRQSEALSKKRMLGKD 240
           DPNYDALIAALYPDIDKYEEEELTFHEEERTRNKQIQESIAQTFQRQSEALSKKRMLGKD
Sbjct: 181 DPNYDALIAALYPDIDKYEEEELTFHEEERTRNKQIQESIAQTFQRQSEALSKKRMLGKD 240

Query: 241 MPGMILTRSRRNHRNVHLRRQNGRGDELLGYEDNEDEDDNSEGKDSSSADERCSKVRQRR 300
           MPGMILTRSRRNHRNVHLRRQNGRGDELLGYEDNEDEDDNSEGKDSSSADERCSKVRQRR
Sbjct: 241 MPGMILTRSRRNHRNVHLRRQNGRGDELLGYEDNEDEDDNSEGKDSSSADERCSKVRQRR 300

Query: 301 KKRHSTVRSFQPSSSIASVDYGCTDGCAESDLDMSRENRTVSPGLVLNSEMLGWGRGGVR 360
           KKRHSTVRSFQPSSSIASV YGCTDGCAESDLDMSRENRTVSPGLVLNSEMLGWGRGGVR
Sbjct: 301 KKRHSTVRSFQPSSSIASVYYGCTDGCAESDLDMSRENRTVSPGLVLNSEMLGWGRGGVR 360

Query: 361 SNTRYGNAGGLSNKGSRSGRLMKLVNYLRGLEENNNELDVHILLISVDSESTPSLKQPHL 420
           SNTRYGNAGGLSNKGSRSGRLMKLVNYLRGLEENNNELDVHILLISVDSESTPSLKQPHL
Sbjct: 361 SNTRYGNAGGLSNKGSRSGRLMKLVNYLRGLEENNNELDVHILLISVDSESTPSLKQPHL 420

Query: 421 QCRPSLTVKHLREYVSRKTPLPADDVELLSVKDRYGTSDAQSTPSSSIFNPLKYELQILG 480
           QCRPSLTVKHLREYVSRKTPLPADDVELLSVKDRYGTSDAQSTPSSSIFNPLKYELQILG
Sbjct: 421 QCRPSLTVKHLREYVSRKTPLPADDVELLSVKDRYGTSDAQSTPSSSIFNPLKYELQILG 480

Query: 481 GEETLAGLQADCTYSREHLILGYRWKGRS 510
           GEETLAGLQADCTYSREHLILGYRWKGRS
Sbjct: 481 GEETLAGLQADCTYSREHLILGYRWKGRS 482

BLAST of Carg13592 vs. NCBI nr
Match: XP_023532552.1 (putative E3 ubiquitin-protein ligase RING1a [Cucurbita pepo subsp. pepo])

HSP 1 Score: 897.9 bits (2319), Expect = 4.1e-257
Identity = 475/510 (93.14%), Postives = 478/510 (93.73%), Query Frame = 0

Query: 1   MPAHKRSWSDSLVGEELSDEQFSHQDAKQCRAERQPNTEV-EEEGEEEQKKPKKEAEREE 60
           MPAHKRSWSDSLVGEELSDEQFSHQDAKQCRAERQPNTEV EEE EEEQKKPK+EAER E
Sbjct: 1   MPAHKRSWSDSLVGEELSDEQFSHQDAKQCRAERQPNTEVEEEEEEEEQKKPKEEAERVE 60

Query: 61  KQHKQLEEGTGARDSEESQSSSSEEKPEYFLLNPSTSFSLYDCHFNGAVDLMCGKFIFVE 120
           KQHKQLEEGTGARDSEESQSSSSEEKPE                           FIFVE
Sbjct: 61  KQHKQLEEGTGARDSEESQSSSSEEKPE---------------------------FIFVE 120

Query: 121 LSGIRKDVQCPICLGIIKKTRTVMECLHRFCRECIDKSMRLGNNECPACRTHCASRRSLR 180
           LSGIRKDVQCPICLGIIKKTRTVMECLHRFCRECIDKSMRLGNNECPACRTHCASRRSLR
Sbjct: 121 LSGIRKDVQCPICLGIIKKTRTVMECLHRFCRECIDKSMRLGNNECPACRTHCASRRSLR 180

Query: 181 DDPNYDALIAALYPDIDKYEEEELTFHEEERTRNKQIQESIAQTFQRQSEALSKKRMLGK 240
           DDPNYDALIAALYPDIDKYEEEELTFHEEERTRNKQIQESIAQTFQRQSEALSKKRMLGK
Sbjct: 181 DDPNYDALIAALYPDIDKYEEEELTFHEEERTRNKQIQESIAQTFQRQSEALSKKRMLGK 240

Query: 241 DMPGMILTRSRRNHRNVHLRRQNGRGDELLGYEDNEDEDDNSEGKDSSSADERCSKVRQR 300
           DMPGMILTRSRRNHRNVHLRRQNGRGDELLGYEDNEDEDDNSEGKDSSSADERCSKVRQR
Sbjct: 241 DMPGMILTRSRRNHRNVHLRRQNGRGDELLGYEDNEDEDDNSEGKDSSSADERCSKVRQR 300

Query: 301 RKKRHSTVRSFQPSSSIASVDYGCTDGCAESDLDMSRENRTVSPGLVLNSEMLGWGRGGV 360
           RKKRHSTVRSFQPSSSIASVDYGCTDGCAESDLDMSRENRTVSPGLVLNSEMLGWGRGGV
Sbjct: 301 RKKRHSTVRSFQPSSSIASVDYGCTDGCAESDLDMSRENRTVSPGLVLNSEMLGWGRGGV 360

Query: 361 RSNTRYGNAGGLSNKGSRSGRLMKLVNYLRGLEENNNELDVHILLISVDSESTPSLKQPH 420
           RSNTRYGNAGGLSNKGSRSGRLMKLVNYLRGLEENNNELDVHILLISVD+E+TPSLKQPH
Sbjct: 361 RSNTRYGNAGGLSNKGSRSGRLMKLVNYLRGLEENNNELDVHILLISVDTENTPSLKQPH 420

Query: 421 LQCRPSLTVKHLREYVSRKTPLPADDVELLSVKDRYGTSDAQSTPSSSIFNPLKYELQIL 480
           LQCRPSLTVKHLREYVSRKTPLPADDVELL VKDRYGTSDAQSTPSSSIFNPLKYELQIL
Sbjct: 421 LQCRPSLTVKHLREYVSRKTPLPADDVELLLVKDRYGTSDAQSTPSSSIFNPLKYELQIL 480

Query: 481 GGEETLAGLQADCTYSREHLILGYRWKGRS 510
           GGEETLAGLQADCTYSR HLILGYRWKGRS
Sbjct: 481 GGEETLAGLQADCTYSRAHLILGYRWKGRS 483

BLAST of Carg13592 vs. NCBI nr
Match: XP_022971159.1 (putative E3 ubiquitin-protein ligase RING1a [Cucurbita maxima])

HSP 1 Score: 891.7 bits (2303), Expect = 2.9e-255
Identity = 468/509 (91.94%), Postives = 475/509 (93.32%), Query Frame = 0

Query: 1   MPAHKRSWSDSLVGEELSDEQFSHQDAKQCRAERQPNTEVEEEGEEEQKKPKKEAEREEK 60
           MPAHKRSWSDSLVGEELSD+QFSHQDAKQ R ERQPNTEVEEE EEEQKKPK+EAER EK
Sbjct: 1   MPAHKRSWSDSLVGEELSDQQFSHQDAKQSRTERQPNTEVEEEEEEEQKKPKEEAERAEK 60

Query: 61  QHKQLEEGTGARDSEESQSSSSEEKPEYFLLNPSTSFSLYDCHFNGAVDLMCGKFIFVEL 120
           QHKQLEEGTGARDSEES+SSSSEEKPE                           FIFVEL
Sbjct: 61  QHKQLEEGTGARDSEESESSSSEEKPE---------------------------FIFVEL 120

Query: 121 SGIRKDVQCPICLGIIKKTRTVMECLHRFCRECIDKSMRLGNNECPACRTHCASRRSLRD 180
           SGIRKDVQCPICLGIIKKTRTVMECLHRFCRECIDKSMRLGNNECPACRTHCASRRSLRD
Sbjct: 121 SGIRKDVQCPICLGIIKKTRTVMECLHRFCRECIDKSMRLGNNECPACRTHCASRRSLRD 180

Query: 181 DPNYDALIAALYPDIDKYEEEELTFHEEERTRNKQIQESIAQTFQRQSEALSKKRMLGKD 240
           DPNYDALIAALYPDIDKYEEEELTFHEEERTRNKQIQESIAQTFQRQSEALSKKRMLGKD
Sbjct: 181 DPNYDALIAALYPDIDKYEEEELTFHEEERTRNKQIQESIAQTFQRQSEALSKKRMLGKD 240

Query: 241 MPGMILTRSRRNHRNVHLRRQNGRGDELLGYEDNEDEDDNSEGKDSSSADERCSKVRQRR 300
           MPGMILTRSRRNHRNVHLRRQNGRGDELLGYEDNEDEDDNSEGKDSSSADERC+KVRQRR
Sbjct: 241 MPGMILTRSRRNHRNVHLRRQNGRGDELLGYEDNEDEDDNSEGKDSSSADERCAKVRQRR 300

Query: 301 KKRHSTVRSFQPSSSIASVDYGCTDGCAESDLDMSRENRTVSPGLVLNSEMLGWGRGGVR 360
           KKRHST+RSFQPSSSIASVDYGCTDGCAESDLDM RENRTVSPGLVLNSEMLGWGRGGVR
Sbjct: 301 KKRHSTIRSFQPSSSIASVDYGCTDGCAESDLDMRRENRTVSPGLVLNSEMLGWGRGGVR 360

Query: 361 SNTRYGNAGGLSNKGSRSGRLMKLVNYLRGLEENNNELDVHILLISVDSESTPSLKQPHL 420
           SNTRYGNAGGLSNKGSRSGRLMKLVNYLRGLEENNNELDVHILLISVD+ESTPSLKQPHL
Sbjct: 361 SNTRYGNAGGLSNKGSRSGRLMKLVNYLRGLEENNNELDVHILLISVDTESTPSLKQPHL 420

Query: 421 QCRPSLTVKHLREYVSRKTPLPADDVELLSVKDRYGTSDAQSTPSSSIFNPLKYELQILG 480
           QCRPSLTVKHLREYVSRKTPLPADDVELLSV+DRYGTSD QSTPSSSIFNPLKYELQIL 
Sbjct: 421 QCRPSLTVKHLREYVSRKTPLPADDVELLSVRDRYGTSDTQSTPSSSIFNPLKYELQILE 480

Query: 481 GEETLAGLQADCTYSREHLILGYRWKGRS 510
           GEETLAGLQADCTYSREHLILGYRWKGRS
Sbjct: 481 GEETLAGLQADCTYSREHLILGYRWKGRS 482

BLAST of Carg13592 vs. NCBI nr
Match: XP_022948045.1 (putative E3 ubiquitin-protein ligase RING1a [Cucurbita moschata])

HSP 1 Score: 888.3 bits (2294), Expect = 3.2e-254
Identity = 474/510 (92.94%), Postives = 476/510 (93.33%), Query Frame = 0

Query: 1   MPAHKRSWSDSLVGEELSDEQFSHQDAKQCRAERQPNTEV-EEEGEEEQKKPKKEAEREE 60
           MPAHKRSWSDSLVGEELSDEQFSHQDAKQCRAERQPNTEV EEE EEEQKKPK+EAER E
Sbjct: 1   MPAHKRSWSDSLVGEELSDEQFSHQDAKQCRAERQPNTEVEEEEEEEEQKKPKEEAERAE 60

Query: 61  KQHKQLEEGTGARDSEESQSSSSEEKPEYFLLNPSTSFSLYDCHFNGAVDLMCGKFIFVE 120
           KQHKQLEEGTGARDSEESQSSSSEEKPE                           FIFVE
Sbjct: 61  KQHKQLEEGTGARDSEESQSSSSEEKPE---------------------------FIFVE 120

Query: 121 LSGIRKDVQCPICLGIIKKTRTVMECLHRFCRECIDKSMRLGNNECPACRTHCASRRSLR 180
           LSGIRKDVQCPICLGIIKKTRTVMECLHRFCRECIDKSMRLGNNECPACRTHCASRRSLR
Sbjct: 121 LSGIRKDVQCPICLGIIKKTRTVMECLHRFCRECIDKSMRLGNNECPACRTHCASRRSLR 180

Query: 181 DDPNYDALIAALYPDIDKYEEEELTFHEEERTRNKQIQESIAQTFQRQSEALSKKRMLGK 240
           DDPNYDALIAALYPDIDKYEEEELTFHEEERTRNKQIQESIAQTFQRQSEALSKKRMLGK
Sbjct: 181 DDPNYDALIAALYPDIDKYEEEELTFHEEERTRNKQIQESIAQTFQRQSEALSKKRMLGK 240

Query: 241 DMPGMILTRSRRNHRNVHLRRQNGRGDELLGYEDNEDEDDNSEGKDSSSADERCSKVRQR 300
           DMPGMILTRSRRNHRNVHLRRQNGRGDELLGYEDNED DDNSEGKDSSSADERC+KVRQR
Sbjct: 241 DMPGMILTRSRRNHRNVHLRRQNGRGDELLGYEDNED-DDNSEGKDSSSADERCAKVRQR 300

Query: 301 RKKRHSTVRSFQPSSSIASVDYGCTDGCAESDLDMSRENRTVSPGLVLNSEMLGWGRGGV 360
           RKKRHSTVRSFQPSSSIASVDYG TDGCAESDLDMSRENRTVSPGLVLNSEMLGWGRGGV
Sbjct: 301 RKKRHSTVRSFQPSSSIASVDYGFTDGCAESDLDMSRENRTVSPGLVLNSEMLGWGRGGV 360

Query: 361 RSNTRYGNAGGLSNKGSRSGRLMKLVNYLRGLEENNNELDVHILLISVDSESTPSLKQPH 420
           RSNTRYGNAGGLSNKGSRSGRLMKLVNYLRGLEENNNELDVHILLISVDSESTPSLKQPH
Sbjct: 361 RSNTRYGNAGGLSNKGSRSGRLMKLVNYLRGLEENNNELDVHILLISVDSESTPSLKQPH 420

Query: 421 LQCRPSLTVKHLREYVSRKTPLPADDVELLSVKDRYGTSDAQSTPSSSIFNPLKYELQIL 480
           LQCRPSLTVK LREYVSRKTPLPADDVELLSVKDRYGT DAQSTPSSSIFNPLKYELQIL
Sbjct: 421 LQCRPSLTVKQLREYVSRKTPLPADDVELLSVKDRYGTIDAQSTPSSSIFNPLKYELQIL 480

Query: 481 GGEETLAGLQADCTYSREHLILGYRWKGRS 510
           GGEETLAGLQADCTYSREHLILGYRWKGRS
Sbjct: 481 GGEETLAGLQADCTYSREHLILGYRWKGRS 482

BLAST of Carg13592 vs. ExPASy Swiss-Prot
Match: Q9FKW0 (Putative E3 ubiquitin-protein ligase RING1a OS=Arabidopsis thaliana OX=3702 GN=RING1A PE=1 SV=2)

HSP 1 Score: 365.9 bits (938), Expect = 7.3e-100
Identity = 239/513 (46.59%), Postives = 331/513 (64.52%), Query Frame = 0

Query: 10  DSLVGEELSDEQFSHQDAKQCRAERQPN-TEVEEEGEEEQKKPKKEAEREEKQHKQLEEG 69
           D    E+  DE+  H +A++ +   +PN  E +++G++ ++  ++E E EE +  + EE 
Sbjct: 36  DETKEEKEGDEEVKHDEAEEDQEVVKPNDAEEDDDGDDAEEDEEEEVEAEEDEEAE-EEE 95

Query: 70  TGARDSEESQSSSSEEKPEYFLLNPSTSFSLYDCHFNGAVDLMCGKFIFVELSGIRKDVQ 129
               + EE +  S E  P     + S                   +F+ ++L  IRKDVQ
Sbjct: 96  EEEEEEEEEEEDSKERSPSSISGDQS-------------------EFMEIDLGEIRKDVQ 155

Query: 130 CPICLGIIKKTRTVMECLHRFCRECIDKSMRLGNNECPACRTHCASRRSLRDDPNYDALI 189
           CPICLGIIKKTRTVMECLHRFCRECIDKSMRLGNNECPACR HCASRRSLRDDP +DALI
Sbjct: 156 CPICLGIIKKTRTVMECLHRFCRECIDKSMRLGNNECPACRKHCASRRSLRDDPKFDALI 215

Query: 190 AALYPDIDKYEEEELTFHEEERTRNKQIQESIAQTFQRQSEALSKKRMLGKDMPGM---- 249
           AAL+ +ID YEEEEL FHE+E  RNKQIQ SIAQ  QRQSEAL K+R LGK+   +    
Sbjct: 216 AALFTNIDSYEEEELAFHEDEMARNKQIQASIAQISQRQSEALVKRRSLGKEAAVLMRSP 275

Query: 250 -ILTRSRRNHRNVHLRRQNGRGDELLGYEDNEDEDDNSEGKD-SSSADERCSKVRQRRKK 309
            I + SRR   + ++ +QN        +ED++++D+N+ G+D  SS+DER ++VRQ++++
Sbjct: 276 RIASGSRRRRNSRNMEQQNASE----AHEDDDNDDNNNRGRDKDSSSDERGTEVRQKKRR 335

Query: 310 RHSTVRSFQ-PSSSIASVDYGCTDGCAESDLDMSRENRTVSPGLVLNSEMLGWGRGGVRS 369
           + ST RS Q PSSS A+ + G    CA++D ++ R+ + +SPGLV N E+L WGRGG RS
Sbjct: 336 KRSTSRSTQHPSSSGANKNNG---NCADNDTEVYRDTKGISPGLVWNPEILAWGRGGTRS 395

Query: 370 NTRYG-NAGGLSNKGSRSGRLMKLVNYLR-GLEENNNELDVHILLISVDSESTPSLKQPH 429
           NTR+G N  G S+K  R+ R+ KLV YLR  ++ ++ ELD+H+ L+S+D++  P L QP+
Sbjct: 396 NTRHGNNTSGGSSKSVRNARVNKLVEYLRSSVDGSSVELDIHVKLVSLDTKCIPDLPQPY 455

Query: 430 LQCRPSLTVKHLREYVSRKTPLPADDVELLSVKDRYGTSDA-----QSTPSSSIFNPLKY 489
           L CRP+L VK LRE+V+ +  L  ++VELL  + R G   A       TP+S+  +  K 
Sbjct: 456 LCCRPTLLVKQLREFVALQIHLKTEEVELLVTRRRVGEDAAIENLPAVTPASAAAS--KD 515

Query: 490 ELQILGGEETLAGLQADCTYSRE-HLILGYRWK 507
           E+  L   ETL+ L+ D   S E HLI+ YR K
Sbjct: 516 EMLSLEDNETLSRLKIDFISSHEQHLIIAYRKK 519

BLAST of Carg13592 vs. ExPASy Swiss-Prot
Match: Q0WX00 (Putative E3 ubiquitin-protein ligase RING1b OS=Arabidopsis thaliana OX=3702 GN=RING1B PE=1 SV=1)

HSP 1 Score: 328.2 bits (840), Expect = 1.7e-88
Identity = 225/473 (47.57%), Postives = 297/473 (62.79%), Query Frame = 0

Query: 37  NTEVEEEGEEEQKKPKKEAEREEKQHKQLEEGTGARDSEESQSSSSEEKPEYFLLNPSTS 96
           N+E E    E  +K +   + +EK     EE    RD  E++   +  + E       +S
Sbjct: 20  NSEAERFNPEAVEKEEDPDKMDEKDESGDEEDDVKRDQVEAEDEEALGEEEDSKERSQSS 79

Query: 97  FSLYDCHFNGAVDLMCGKFIFVELSGIRKDVQCPICLGIIKKTRTVMECLHRFCRECIDK 156
                     A +L   +++ V+L+ I KDVQC ICLGII+KTRTVMECLHRFCRECIDK
Sbjct: 80  ---------SAGELSESEYMVVDLADICKDVQCSICLGIIRKTRTVMECLHRFCRECIDK 139

Query: 157 SMRLGNNECPACRTHCASRRSLRDDPNYDALIAALYPDIDKYEEEELTFHEEERTRNKQI 216
           SMRLGNNECP CR HCASRRSLRDDPN+DALIAAL+ +IDK+EEEEL F +++  RNKQI
Sbjct: 140 SMRLGNNECPTCRKHCASRRSLRDDPNFDALIAALFKNIDKFEEEELNFRQDDEARNKQI 199

Query: 217 QESIAQTFQRQSEALSKKRMLGKDMPGMILTRSRRNHRNVHLRRQNGRGDELLGYEDNED 276
           Q SIAQ  QRQS+AL K++ +GK     IL+RSRR+      RR+N R  E    E N+D
Sbjct: 200 QASIAQVSQRQSKALVKRKSVGKGT--AILSRSRRSGGGSR-RRRNCRNIEQDTSEANDD 259

Query: 277 EDDNSEGKDSSSADERCSKVRQRRKKRHSTVRSFQPSSSIASVDYGCTDGCAESDLDMSR 336
           +D N  GKDSSS DE C +   +RKKR +T    QPSSS A+ +  C     E      R
Sbjct: 260 DDQNKRGKDSSS-DEPCER---QRKKRSAT----QPSSSNANNNDNCAGNGTEQ--THQR 319

Query: 337 ENRTVSPGLVLNSEMLGWGRGGVRSNTRYGNAGGLSNKGS---RSGRLMKLVNYLRGLEE 396
           ++R +SP LV NSE++ WGRGG RSNTR GN    +N+G+   R+ RL +LV YL  LE 
Sbjct: 320 DSRVISPVLVWNSELIAWGRGGTRSNTRQGN----NNQGAISKRNARLKRLVEYLGSLEG 379

Query: 397 NNNELDVHILLISVDSESTPSLKQPHLQCRPSLTVKHLREYVSRKTPLPADDVELLSVKD 456
           N+ ELD+H+ L+S+D+E   +L +P+L  RP+L VK LREYV+R   L A++VELL  KD
Sbjct: 380 NSVELDIHLKLVSLDTEGLLNLHEPYLCFRPTLLVKQLREYVARHLKLKAEEVELLVSKD 439

Query: 457 RYGTSDAQSTPSSSIFNPLKYELQILGGEETLAGLQADCTYSREHLILGYRWK 507
             G +   +  S+        ++Q L  +ET+A L+ DC  S  ++I+ YR K
Sbjct: 440 --GDTVIGNKTSTE-------KMQSLQDDETVAKLKVDCISSNGYMIVVYRRK 457

BLAST of Carg13592 vs. ExPASy Swiss-Prot
Match: Q66J69 (E3 ubiquitin-protein ligase RING2-A OS=Xenopus laevis OX=8355 GN=rnf2-a PE=2 SV=1)

HSP 1 Score: 108.2 bits (269), Expect = 2.7e-22
Identity = 56/133 (42.11%), Postives = 79/133 (59.40%), Query Frame = 0

Query: 98  SLYDCHFNGAVDLMCGKFIFVELSGIRKDVQCPICLGIIKKTRTVMECLHRFCRECIDKS 157
           SLY+ H      +M G  I V    +  ++ CPICL ++K T T  ECLHRFC +CI  +
Sbjct: 17  SLYELHRTPQEAIMDGTEIAVSPRSLHSELMCPICLDMLKNTMTTKECLHRFCSDCIVTA 76

Query: 158 MRLGNNECPACRTHCASRRSLRDDPNYDALIAALYPDIDKYE-EEELTFHEEERTRNKQ- 217
           +R GN ECP CR    S+RSLR DPN+DALI+ +YP  D+YE  ++    +  R  N+Q 
Sbjct: 77  LRSGNKECPTCRKKLVSKRSLRPDPNFDALISKIYPSRDEYEAHQDRVLAKLNRLHNQQA 136

Query: 218 IQESIAQTFQRQS 229
           +  SI +  + Q+
Sbjct: 137 LSSSIEEGLKMQA 149

BLAST of Carg13592 vs. ExPASy Swiss-Prot
Match: Q5TJF3 (E3 ubiquitin-protein ligase RING1 OS=Canis lupus familiaris OX=9615 GN=RING1 PE=3 SV=2)

HSP 1 Score: 105.1 bits (261), Expect = 2.3e-21
Identity = 66/185 (35.68%), Postives = 95/185 (51.35%), Query Frame = 0

Query: 98  SLYDCHFNGAVDLMCGKFIFVELSGIRKDVQCPICLGIIKKTRTVMECLHRFCRECIDKS 157
           SLY+ H      +M G  I V    +  ++ CPICL ++K T T  ECLHRFC +CI  +
Sbjct: 17  SLYELHRTPQEAIMDGTEIAVSPRSLHSELMCPICLDMLKNTMTTKECLHRFCSDCIVTA 76

Query: 158 MRLGNNECPACRTHCASRRSLRDDPNYDALIAALYPDIDKYE-EEELTFHEEERTRNKQI 217
           +R GN ECP CR    S+RSLR DPN+DALI+ +YP  ++YE  ++       R  N+Q 
Sbjct: 77  LRSGNKECPTCRKKLVSKRSLRPDPNFDALISKIYPSREEYEAHQDRVLIRLSRLHNQQA 136

Query: 218 QESIAQTFQRQSEALSKKRMLGKDMPGMILTRSRRNHRNVHLRRQNGRGDELLGYEDNED 277
             S  +   R  +A+ + + + + MPG   T +             G G+   G  D ED
Sbjct: 137 LSSSIEEGLRM-QAMHRAQRVRRPMPGSDQTTT----------MSGGEGEPGEGEGDGED 190

Query: 278 EDDNS 282
              +S
Sbjct: 197 VSSDS 190

BLAST of Carg13592 vs. ExPASy Swiss-Prot
Match: O35730 (E3 ubiquitin-protein ligase RING1 OS=Mus musculus OX=10090 GN=Ring1 PE=1 SV=2)

HSP 1 Score: 105.1 bits (261), Expect = 2.3e-21
Identity = 66/185 (35.68%), Postives = 95/185 (51.35%), Query Frame = 0

Query: 98  SLYDCHFNGAVDLMCGKFIFVELSGIRKDVQCPICLGIIKKTRTVMECLHRFCRECIDKS 157
           SLY+ H      +M G  I V    +  ++ CPICL ++K T T  ECLHRFC +CI  +
Sbjct: 17  SLYELHRTPQEAIMDGTEIAVSPRSLHSELMCPICLDMLKNTMTTKECLHRFCSDCIVTA 76

Query: 158 MRLGNNECPACRTHCASRRSLRDDPNYDALIAALYPDIDKYE-EEELTFHEEERTRNKQI 217
           +R GN ECP CR    S+RSLR DPN+DALI+ +YP  ++YE  ++       R  N+Q 
Sbjct: 77  LRSGNKECPTCRKKLVSKRSLRPDPNFDALISKIYPSREEYEAHQDRVLIRLSRLHNQQA 136

Query: 218 QESIAQTFQRQSEALSKKRMLGKDMPGMILTRSRRNHRNVHLRRQNGRGDELLGYEDNED 277
             S  +   R  +A+ + + + + MPG   T +             G G+   G  D ED
Sbjct: 137 LSSSIEEGLRM-QAMHRAQRVRRPMPGSDQTAT----------MSGGEGEPGEGEGDGED 190

Query: 278 EDDNS 282
              +S
Sbjct: 197 VSSDS 190

BLAST of Carg13592 vs. ExPASy TrEMBL
Match: A0A6J1I7S3 (putative E3 ubiquitin-protein ligase RING1a OS=Cucurbita maxima OX=3661 GN=LOC111469921 PE=4 SV=1)

HSP 1 Score: 891.7 bits (2303), Expect = 1.4e-255
Identity = 468/509 (91.94%), Postives = 475/509 (93.32%), Query Frame = 0

Query: 1   MPAHKRSWSDSLVGEELSDEQFSHQDAKQCRAERQPNTEVEEEGEEEQKKPKKEAEREEK 60
           MPAHKRSWSDSLVGEELSD+QFSHQDAKQ R ERQPNTEVEEE EEEQKKPK+EAER EK
Sbjct: 1   MPAHKRSWSDSLVGEELSDQQFSHQDAKQSRTERQPNTEVEEEEEEEQKKPKEEAERAEK 60

Query: 61  QHKQLEEGTGARDSEESQSSSSEEKPEYFLLNPSTSFSLYDCHFNGAVDLMCGKFIFVEL 120
           QHKQLEEGTGARDSEES+SSSSEEKPE                           FIFVEL
Sbjct: 61  QHKQLEEGTGARDSEESESSSSEEKPE---------------------------FIFVEL 120

Query: 121 SGIRKDVQCPICLGIIKKTRTVMECLHRFCRECIDKSMRLGNNECPACRTHCASRRSLRD 180
           SGIRKDVQCPICLGIIKKTRTVMECLHRFCRECIDKSMRLGNNECPACRTHCASRRSLRD
Sbjct: 121 SGIRKDVQCPICLGIIKKTRTVMECLHRFCRECIDKSMRLGNNECPACRTHCASRRSLRD 180

Query: 181 DPNYDALIAALYPDIDKYEEEELTFHEEERTRNKQIQESIAQTFQRQSEALSKKRMLGKD 240
           DPNYDALIAALYPDIDKYEEEELTFHEEERTRNKQIQESIAQTFQRQSEALSKKRMLGKD
Sbjct: 181 DPNYDALIAALYPDIDKYEEEELTFHEEERTRNKQIQESIAQTFQRQSEALSKKRMLGKD 240

Query: 241 MPGMILTRSRRNHRNVHLRRQNGRGDELLGYEDNEDEDDNSEGKDSSSADERCSKVRQRR 300
           MPGMILTRSRRNHRNVHLRRQNGRGDELLGYEDNEDEDDNSEGKDSSSADERC+KVRQRR
Sbjct: 241 MPGMILTRSRRNHRNVHLRRQNGRGDELLGYEDNEDEDDNSEGKDSSSADERCAKVRQRR 300

Query: 301 KKRHSTVRSFQPSSSIASVDYGCTDGCAESDLDMSRENRTVSPGLVLNSEMLGWGRGGVR 360
           KKRHST+RSFQPSSSIASVDYGCTDGCAESDLDM RENRTVSPGLVLNSEMLGWGRGGVR
Sbjct: 301 KKRHSTIRSFQPSSSIASVDYGCTDGCAESDLDMRRENRTVSPGLVLNSEMLGWGRGGVR 360

Query: 361 SNTRYGNAGGLSNKGSRSGRLMKLVNYLRGLEENNNELDVHILLISVDSESTPSLKQPHL 420
           SNTRYGNAGGLSNKGSRSGRLMKLVNYLRGLEENNNELDVHILLISVD+ESTPSLKQPHL
Sbjct: 361 SNTRYGNAGGLSNKGSRSGRLMKLVNYLRGLEENNNELDVHILLISVDTESTPSLKQPHL 420

Query: 421 QCRPSLTVKHLREYVSRKTPLPADDVELLSVKDRYGTSDAQSTPSSSIFNPLKYELQILG 480
           QCRPSLTVKHLREYVSRKTPLPADDVELLSV+DRYGTSD QSTPSSSIFNPLKYELQIL 
Sbjct: 421 QCRPSLTVKHLREYVSRKTPLPADDVELLSVRDRYGTSDTQSTPSSSIFNPLKYELQILE 480

Query: 481 GEETLAGLQADCTYSREHLILGYRWKGRS 510
           GEETLAGLQADCTYSREHLILGYRWKGRS
Sbjct: 481 GEETLAGLQADCTYSREHLILGYRWKGRS 482

BLAST of Carg13592 vs. ExPASy TrEMBL
Match: A0A6J1G8L3 (putative E3 ubiquitin-protein ligase RING1a OS=Cucurbita moschata OX=3662 GN=LOC111451742 PE=4 SV=1)

HSP 1 Score: 888.3 bits (2294), Expect = 1.6e-254
Identity = 474/510 (92.94%), Postives = 476/510 (93.33%), Query Frame = 0

Query: 1   MPAHKRSWSDSLVGEELSDEQFSHQDAKQCRAERQPNTEV-EEEGEEEQKKPKKEAEREE 60
           MPAHKRSWSDSLVGEELSDEQFSHQDAKQCRAERQPNTEV EEE EEEQKKPK+EAER E
Sbjct: 1   MPAHKRSWSDSLVGEELSDEQFSHQDAKQCRAERQPNTEVEEEEEEEEQKKPKEEAERAE 60

Query: 61  KQHKQLEEGTGARDSEESQSSSSEEKPEYFLLNPSTSFSLYDCHFNGAVDLMCGKFIFVE 120
           KQHKQLEEGTGARDSEESQSSSSEEKPE                           FIFVE
Sbjct: 61  KQHKQLEEGTGARDSEESQSSSSEEKPE---------------------------FIFVE 120

Query: 121 LSGIRKDVQCPICLGIIKKTRTVMECLHRFCRECIDKSMRLGNNECPACRTHCASRRSLR 180
           LSGIRKDVQCPICLGIIKKTRTVMECLHRFCRECIDKSMRLGNNECPACRTHCASRRSLR
Sbjct: 121 LSGIRKDVQCPICLGIIKKTRTVMECLHRFCRECIDKSMRLGNNECPACRTHCASRRSLR 180

Query: 181 DDPNYDALIAALYPDIDKYEEEELTFHEEERTRNKQIQESIAQTFQRQSEALSKKRMLGK 240
           DDPNYDALIAALYPDIDKYEEEELTFHEEERTRNKQIQESIAQTFQRQSEALSKKRMLGK
Sbjct: 181 DDPNYDALIAALYPDIDKYEEEELTFHEEERTRNKQIQESIAQTFQRQSEALSKKRMLGK 240

Query: 241 DMPGMILTRSRRNHRNVHLRRQNGRGDELLGYEDNEDEDDNSEGKDSSSADERCSKVRQR 300
           DMPGMILTRSRRNHRNVHLRRQNGRGDELLGYEDNED DDNSEGKDSSSADERC+KVRQR
Sbjct: 241 DMPGMILTRSRRNHRNVHLRRQNGRGDELLGYEDNED-DDNSEGKDSSSADERCAKVRQR 300

Query: 301 RKKRHSTVRSFQPSSSIASVDYGCTDGCAESDLDMSRENRTVSPGLVLNSEMLGWGRGGV 360
           RKKRHSTVRSFQPSSSIASVDYG TDGCAESDLDMSRENRTVSPGLVLNSEMLGWGRGGV
Sbjct: 301 RKKRHSTVRSFQPSSSIASVDYGFTDGCAESDLDMSRENRTVSPGLVLNSEMLGWGRGGV 360

Query: 361 RSNTRYGNAGGLSNKGSRSGRLMKLVNYLRGLEENNNELDVHILLISVDSESTPSLKQPH 420
           RSNTRYGNAGGLSNKGSRSGRLMKLVNYLRGLEENNNELDVHILLISVDSESTPSLKQPH
Sbjct: 361 RSNTRYGNAGGLSNKGSRSGRLMKLVNYLRGLEENNNELDVHILLISVDSESTPSLKQPH 420

Query: 421 LQCRPSLTVKHLREYVSRKTPLPADDVELLSVKDRYGTSDAQSTPSSSIFNPLKYELQIL 480
           LQCRPSLTVK LREYVSRKTPLPADDVELLSVKDRYGT DAQSTPSSSIFNPLKYELQIL
Sbjct: 421 LQCRPSLTVKQLREYVSRKTPLPADDVELLSVKDRYGTIDAQSTPSSSIFNPLKYELQIL 480

Query: 481 GGEETLAGLQADCTYSREHLILGYRWKGRS 510
           GGEETLAGLQADCTYSREHLILGYRWKGRS
Sbjct: 481 GGEETLAGLQADCTYSREHLILGYRWKGRS 482

BLAST of Carg13592 vs. ExPASy TrEMBL
Match: A0A6J1FTH1 (putative E3 ubiquitin-protein ligase RING1b isoform X1 OS=Cucurbita moschata OX=3662 GN=LOC111448324 PE=4 SV=1)

HSP 1 Score: 771.5 bits (1991), Expect = 2.1e-219
Identity = 420/531 (79.10%), Postives = 445/531 (83.80%), Query Frame = 0

Query: 1   MPAHKRSWSDSLVGEELSDEQFSHQDAKQCRAERQPNTEVEEE---GEEEQKKPKKEAER 60
           MPAHKRSWSDS+ GEE  D+QFSHQDAKQ R ER+P TE EEE    EEEQ++  KE ER
Sbjct: 1   MPAHKRSWSDSVNGEESGDQQFSHQDAKQSRMEREPKTETEEEEEVEEEEQREHLKEGER 60

Query: 61  -----------EEKQHKQLEEGTGARDSEESQSSSSEEKPEYFLLNPSTSFSLYDCHFNG 120
                      EEKQH QLEE  GARDSE SQSSSSEEKPEYFLLNPS            
Sbjct: 61  EDEGREEDEGEEEKQHNQLEEAAGARDSEGSQSSSSEEKPEYFLLNPS------------ 120

Query: 121 AVDLMCGKFIFVELSGIRKDVQCPICLGIIKKTRTVMECLHRFCRECIDKSMRLGNNECP 180
                   FIFVELSGIRKDVQCPICLGIIKKTRTVMECLHRFCRECIDKSMRLGNNECP
Sbjct: 121 --------FIFVELSGIRKDVQCPICLGIIKKTRTVMECLHRFCRECIDKSMRLGNNECP 180

Query: 181 ACRTHCASRRSLRDDPNYDALIAALYPDIDKYEEEELTFHEEERTRNKQIQESIAQTFQR 240
           ACRTHCASRRSLRDDPNYDALIAALYPDIDKYEEEELTFHEEER RNKQIQESIAQ FQR
Sbjct: 181 ACRTHCASRRSLRDDPNYDALIAALYPDIDKYEEEELTFHEEERNRNKQIQESIAQIFQR 240

Query: 241 QSEALSKKRMLGKDMPGMILTRSRRNHRNVHLRRQNGRGDELLGYEDNEDEDDNSEGKDS 300
           QSEALSKKR+LGKD   + LTRSRRNHRN HLRRQNGRGDE+LGYEDNEDEDDN+EGKDS
Sbjct: 241 QSEALSKKRILGKDTAAVFLTRSRRNHRNAHLRRQNGRGDEVLGYEDNEDEDDNNEGKDS 300

Query: 301 SSADERCSKVRQRRKKRHSTVRSFQPSSSIASVDYGCTDGCAESDLDMSRENRTVSPGLV 360
           SSADER ++VRQRRKKRH TV S QPSSSIA+VD GCTDGCAES+LDMSRENRTVSPGLV
Sbjct: 301 SSADERFTEVRQRRKKRHPTVPSSQPSSSIANVDSGCTDGCAESELDMSRENRTVSPGLV 360

Query: 361 LNSEMLGWGRGGVRSNTRYGNAGGLSNKGSRSGRLMKLVNYLRGLEENNNELDVHILLIS 420
           LNSEMLGWGRGGVRSN R+ +AGG SNK SRS RL KLVNYLRGLEENNNELDVH+LLIS
Sbjct: 361 LNSEMLGWGRGGVRSNARHSSAGGSSNKSSRSSRLTKLVNYLRGLEENNNELDVHLLLIS 420

Query: 421 VDSESTPSLKQPHLQCRPSLTVKHLREYVSRKTPLPADDVELLSVKDRYGTSDAQSTPSS 480
           VD ES PSL+QPHL CRPSLTV+HLREYVSRKTPL ADDVE+LS+K R+ TSD Q+TPS+
Sbjct: 421 VDKESAPSLQQPHLHCRPSLTVEHLREYVSRKTPLQADDVEILSMKARHSTSDVQTTPSA 480

Query: 481 SI--------FNPLKYELQILGGEETLAGLQADCTYSREHLILGYRWKGRS 510
           SI        F+PLKYELQ L GEETLAGLQADC YSR+HLILGYR KGRS
Sbjct: 481 SISIDGTSLVFDPLKYELQTLEGEETLAGLQADCIYSRDHLILGYRRKGRS 511

BLAST of Carg13592 vs. ExPASy TrEMBL
Match: A0A6J1J9P4 (putative E3 ubiquitin-protein ligase RING1a isoform X1 OS=Cucurbita maxima OX=3661 GN=LOC111483842 PE=4 SV=1)

HSP 1 Score: 770.4 bits (1988), Expect = 4.7e-219
Identity = 419/531 (78.91%), Postives = 445/531 (83.80%), Query Frame = 0

Query: 1   MPAHKRSWSDSLVGEELSDEQFSHQDAKQCRAERQPNTEVEEE---GEEEQKKPKKEAER 60
           MPAHKRSWSDS+ GEE  D+QFSHQDAKQ R ER+P TE EEE    EEEQ++  KE ER
Sbjct: 1   MPAHKRSWSDSVNGEESGDQQFSHQDAKQSRMEREPKTETEEEEEVEEEEQREHLKEGER 60

Query: 61  -----------EEKQHKQLEEGTGARDSEESQSSSSEEKPEYFLLNPSTSFSLYDCHFNG 120
                      EEKQH QLEE  GARDSE SQSSSSEEKPEYFLLNPS            
Sbjct: 61  EDEEREEDEGEEEKQHNQLEEAAGARDSEGSQSSSSEEKPEYFLLNPS------------ 120

Query: 121 AVDLMCGKFIFVELSGIRKDVQCPICLGIIKKTRTVMECLHRFCRECIDKSMRLGNNECP 180
                   FIFVELSGIRKDVQCPICLGIIKKTRTVMECLHRFCRECIDKSMRLGNNECP
Sbjct: 121 --------FIFVELSGIRKDVQCPICLGIIKKTRTVMECLHRFCRECIDKSMRLGNNECP 180

Query: 181 ACRTHCASRRSLRDDPNYDALIAALYPDIDKYEEEELTFHEEERTRNKQIQESIAQTFQR 240
           ACRTHCASRRSLRDDPNYDALI+ALYPDIDKYEEEELTFHEEER RNKQIQESIAQ FQR
Sbjct: 181 ACRTHCASRRSLRDDPNYDALISALYPDIDKYEEEELTFHEEERNRNKQIQESIAQIFQR 240

Query: 241 QSEALSKKRMLGKDMPGMILTRSRRNHRNVHLRRQNGRGDELLGYEDNEDEDDNSEGKDS 300
           QSEALSKKR+LGKD   + LTRSRRNHRN HLRRQNGRGDE+LGYEDNEDEDDN+EGKDS
Sbjct: 241 QSEALSKKRILGKDTAAVFLTRSRRNHRNAHLRRQNGRGDEVLGYEDNEDEDDNNEGKDS 300

Query: 301 SSADERCSKVRQRRKKRHSTVRSFQPSSSIASVDYGCTDGCAESDLDMSRENRTVSPGLV 360
           SSADER ++VRQRRKKRH TVRS QPSSSIA+VD GCTDGCAES+LDMSRENRTVSPGLV
Sbjct: 301 SSADERFTEVRQRRKKRHPTVRSSQPSSSIANVDSGCTDGCAESELDMSRENRTVSPGLV 360

Query: 361 LNSEMLGWGRGGVRSNTRYGNAGGLSNKGSRSGRLMKLVNYLRGLEENNNELDVHILLIS 420
           LNSEMLGWGRGGVRSN R+ +AGG SNK SRS RL KLVNYLRGLEENNNELDVH+LLIS
Sbjct: 361 LNSEMLGWGRGGVRSNARHSSAGGSSNKSSRSSRLTKLVNYLRGLEENNNELDVHLLLIS 420

Query: 421 VDSESTPSLKQPHLQCRPSLTVKHLREYVSRKTPLPADDVELLSVKDRYGTSDAQSTPSS 480
           VD ES PSL+QPHL CRPSLTV+HLREYVSRKTPL ADDVE+LS+K R+ TSD Q+TPS+
Sbjct: 421 VDKESAPSLQQPHLHCRPSLTVEHLREYVSRKTPLQADDVEILSMKARHSTSDVQTTPSA 480

Query: 481 SI--------FNPLKYELQILGGEETLAGLQADCTYSREHLILGYRWKGRS 510
           SI        F+PLKYELQ L GEETLAGLQADC YSR+HLILGYR KG S
Sbjct: 481 SISIDGTSLVFDPLKYELQTLEGEETLAGLQADCIYSRDHLILGYRRKGLS 511

BLAST of Carg13592 vs. ExPASy TrEMBL
Match: A0A6J1FY08 (putative E3 ubiquitin-protein ligase RING1a isoform X2 OS=Cucurbita moschata OX=3662 GN=LOC111448324 PE=4 SV=1)

HSP 1 Score: 753.8 bits (1945), Expect = 4.6e-214
Identity = 413/531 (77.78%), Postives = 438/531 (82.49%), Query Frame = 0

Query: 1   MPAHKRSWSDSLVGEELSDEQFSHQDAKQCRAERQPNTEVEEE---GEEEQKKPKKEAER 60
           MPAHKRSWSDS+ GEE  D+QFSHQDAKQ R ER+P TE EEE    EEEQ++  KE ER
Sbjct: 1   MPAHKRSWSDSVNGEESGDQQFSHQDAKQSRMEREPKTETEEEEEVEEEEQREHLKEGER 60

Query: 61  -----------EEKQHKQLEEGTGARDSEESQSSSSEEKPEYFLLNPSTSFSLYDCHFNG 120
                      EEKQH QLEE  GARDSE SQSSSSEEKPE                   
Sbjct: 61  EDEGREEDEGEEEKQHNQLEEAAGARDSEGSQSSSSEEKPE------------------- 120

Query: 121 AVDLMCGKFIFVELSGIRKDVQCPICLGIIKKTRTVMECLHRFCRECIDKSMRLGNNECP 180
                   FIFVELSGIRKDVQCPICLGIIKKTRTVMECLHRFCRECIDKSMRLGNNECP
Sbjct: 121 --------FIFVELSGIRKDVQCPICLGIIKKTRTVMECLHRFCRECIDKSMRLGNNECP 180

Query: 181 ACRTHCASRRSLRDDPNYDALIAALYPDIDKYEEEELTFHEEERTRNKQIQESIAQTFQR 240
           ACRTHCASRRSLRDDPNYDALIAALYPDIDKYEEEELTFHEEER RNKQIQESIAQ FQR
Sbjct: 181 ACRTHCASRRSLRDDPNYDALIAALYPDIDKYEEEELTFHEEERNRNKQIQESIAQIFQR 240

Query: 241 QSEALSKKRMLGKDMPGMILTRSRRNHRNVHLRRQNGRGDELLGYEDNEDEDDNSEGKDS 300
           QSEALSKKR+LGKD   + LTRSRRNHRN HLRRQNGRGDE+LGYEDNEDEDDN+EGKDS
Sbjct: 241 QSEALSKKRILGKDTAAVFLTRSRRNHRNAHLRRQNGRGDEVLGYEDNEDEDDNNEGKDS 300

Query: 301 SSADERCSKVRQRRKKRHSTVRSFQPSSSIASVDYGCTDGCAESDLDMSRENRTVSPGLV 360
           SSADER ++VRQRRKKRH TV S QPSSSIA+VD GCTDGCAES+LDMSRENRTVSPGLV
Sbjct: 301 SSADERFTEVRQRRKKRHPTVPSSQPSSSIANVDSGCTDGCAESELDMSRENRTVSPGLV 360

Query: 361 LNSEMLGWGRGGVRSNTRYGNAGGLSNKGSRSGRLMKLVNYLRGLEENNNELDVHILLIS 420
           LNSEMLGWGRGGVRSN R+ +AGG SNK SRS RL KLVNYLRGLEENNNELDVH+LLIS
Sbjct: 361 LNSEMLGWGRGGVRSNARHSSAGGSSNKSSRSSRLTKLVNYLRGLEENNNELDVHLLLIS 420

Query: 421 VDSESTPSLKQPHLQCRPSLTVKHLREYVSRKTPLPADDVELLSVKDRYGTSDAQSTPSS 480
           VD ES PSL+QPHL CRPSLTV+HLREYVSRKTPL ADDVE+LS+K R+ TSD Q+TPS+
Sbjct: 421 VDKESAPSLQQPHLHCRPSLTVEHLREYVSRKTPLQADDVEILSMKARHSTSDVQTTPSA 480

Query: 481 SI--------FNPLKYELQILGGEETLAGLQADCTYSREHLILGYRWKGRS 510
           SI        F+PLKYELQ L GEETLAGLQADC YSR+HLILGYR KGRS
Sbjct: 481 SISIDGTSLVFDPLKYELQTLEGEETLAGLQADCIYSRDHLILGYRRKGRS 504

BLAST of Carg13592 vs. TAIR 10
Match: AT5G44280.1 (RING 1A )

HSP 1 Score: 365.9 bits (938), Expect = 5.2e-101
Identity = 239/513 (46.59%), Postives = 331/513 (64.52%), Query Frame = 0

Query: 10  DSLVGEELSDEQFSHQDAKQCRAERQPN-TEVEEEGEEEQKKPKKEAEREEKQHKQLEEG 69
           D    E+  DE+  H +A++ +   +PN  E +++G++ ++  ++E E EE +  + EE 
Sbjct: 36  DETKEEKEGDEEVKHDEAEEDQEVVKPNDAEEDDDGDDAEEDEEEEVEAEEDEEAE-EEE 95

Query: 70  TGARDSEESQSSSSEEKPEYFLLNPSTSFSLYDCHFNGAVDLMCGKFIFVELSGIRKDVQ 129
               + EE +  S E  P     + S                   +F+ ++L  IRKDVQ
Sbjct: 96  EEEEEEEEEEEDSKERSPSSISGDQS-------------------EFMEIDLGEIRKDVQ 155

Query: 130 CPICLGIIKKTRTVMECLHRFCRECIDKSMRLGNNECPACRTHCASRRSLRDDPNYDALI 189
           CPICLGIIKKTRTVMECLHRFCRECIDKSMRLGNNECPACR HCASRRSLRDDP +DALI
Sbjct: 156 CPICLGIIKKTRTVMECLHRFCRECIDKSMRLGNNECPACRKHCASRRSLRDDPKFDALI 215

Query: 190 AALYPDIDKYEEEELTFHEEERTRNKQIQESIAQTFQRQSEALSKKRMLGKDMPGM---- 249
           AAL+ +ID YEEEEL FHE+E  RNKQIQ SIAQ  QRQSEAL K+R LGK+   +    
Sbjct: 216 AALFTNIDSYEEEELAFHEDEMARNKQIQASIAQISQRQSEALVKRRSLGKEAAVLMRSP 275

Query: 250 -ILTRSRRNHRNVHLRRQNGRGDELLGYEDNEDEDDNSEGKD-SSSADERCSKVRQRRKK 309
            I + SRR   + ++ +QN        +ED++++D+N+ G+D  SS+DER ++VRQ++++
Sbjct: 276 RIASGSRRRRNSRNMEQQNASE----AHEDDDNDDNNNRGRDKDSSSDERGTEVRQKKRR 335

Query: 310 RHSTVRSFQ-PSSSIASVDYGCTDGCAESDLDMSRENRTVSPGLVLNSEMLGWGRGGVRS 369
           + ST RS Q PSSS A+ + G    CA++D ++ R+ + +SPGLV N E+L WGRGG RS
Sbjct: 336 KRSTSRSTQHPSSSGANKNNG---NCADNDTEVYRDTKGISPGLVWNPEILAWGRGGTRS 395

Query: 370 NTRYG-NAGGLSNKGSRSGRLMKLVNYLR-GLEENNNELDVHILLISVDSESTPSLKQPH 429
           NTR+G N  G S+K  R+ R+ KLV YLR  ++ ++ ELD+H+ L+S+D++  P L QP+
Sbjct: 396 NTRHGNNTSGGSSKSVRNARVNKLVEYLRSSVDGSSVELDIHVKLVSLDTKCIPDLPQPY 455

Query: 430 LQCRPSLTVKHLREYVSRKTPLPADDVELLSVKDRYGTSDA-----QSTPSSSIFNPLKY 489
           L CRP+L VK LRE+V+ +  L  ++VELL  + R G   A       TP+S+  +  K 
Sbjct: 456 LCCRPTLLVKQLREFVALQIHLKTEEVELLVTRRRVGEDAAIENLPAVTPASAAAS--KD 515

Query: 490 ELQILGGEETLAGLQADCTYSRE-HLILGYRWK 507
           E+  L   ETL+ L+ D   S E HLI+ YR K
Sbjct: 516 EMLSLEDNETLSRLKIDFISSHEQHLIIAYRKK 519

BLAST of Carg13592 vs. TAIR 10
Match: AT1G03770.1 (RING 1B )

HSP 1 Score: 328.2 bits (840), Expect = 1.2e-89
Identity = 225/473 (47.57%), Postives = 297/473 (62.79%), Query Frame = 0

Query: 37  NTEVEEEGEEEQKKPKKEAEREEKQHKQLEEGTGARDSEESQSSSSEEKPEYFLLNPSTS 96
           N+E E    E  +K +   + +EK     EE    RD  E++   +  + E       +S
Sbjct: 20  NSEAERFNPEAVEKEEDPDKMDEKDESGDEEDDVKRDQVEAEDEEALGEEEDSKERSQSS 79

Query: 97  FSLYDCHFNGAVDLMCGKFIFVELSGIRKDVQCPICLGIIKKTRTVMECLHRFCRECIDK 156
                     A +L   +++ V+L+ I KDVQC ICLGII+KTRTVMECLHRFCRECIDK
Sbjct: 80  ---------SAGELSESEYMVVDLADICKDVQCSICLGIIRKTRTVMECLHRFCRECIDK 139

Query: 157 SMRLGNNECPACRTHCASRRSLRDDPNYDALIAALYPDIDKYEEEELTFHEEERTRNKQI 216
           SMRLGNNECP CR HCASRRSLRDDPN+DALIAAL+ +IDK+EEEEL F +++  RNKQI
Sbjct: 140 SMRLGNNECPTCRKHCASRRSLRDDPNFDALIAALFKNIDKFEEEELNFRQDDEARNKQI 199

Query: 217 QESIAQTFQRQSEALSKKRMLGKDMPGMILTRSRRNHRNVHLRRQNGRGDELLGYEDNED 276
           Q SIAQ  QRQS+AL K++ +GK     IL+RSRR+      RR+N R  E    E N+D
Sbjct: 200 QASIAQVSQRQSKALVKRKSVGKGT--AILSRSRRSGGGSR-RRRNCRNIEQDTSEANDD 259

Query: 277 EDDNSEGKDSSSADERCSKVRQRRKKRHSTVRSFQPSSSIASVDYGCTDGCAESDLDMSR 336
           +D N  GKDSSS DE C +   +RKKR +T    QPSSS A+ +  C     E      R
Sbjct: 260 DDQNKRGKDSSS-DEPCER---QRKKRSAT----QPSSSNANNNDNCAGNGTEQ--THQR 319

Query: 337 ENRTVSPGLVLNSEMLGWGRGGVRSNTRYGNAGGLSNKGS---RSGRLMKLVNYLRGLEE 396
           ++R +SP LV NSE++ WGRGG RSNTR GN    +N+G+   R+ RL +LV YL  LE 
Sbjct: 320 DSRVISPVLVWNSELIAWGRGGTRSNTRQGN----NNQGAISKRNARLKRLVEYLGSLEG 379

Query: 397 NNNELDVHILLISVDSESTPSLKQPHLQCRPSLTVKHLREYVSRKTPLPADDVELLSVKD 456
           N+ ELD+H+ L+S+D+E   +L +P+L  RP+L VK LREYV+R   L A++VELL  KD
Sbjct: 380 NSVELDIHLKLVSLDTEGLLNLHEPYLCFRPTLLVKQLREYVARHLKLKAEEVELLVSKD 439

Query: 457 RYGTSDAQSTPSSSIFNPLKYELQILGGEETLAGLQADCTYSREHLILGYRWK 507
             G +   +  S+        ++Q L  +ET+A L+ DC  S  ++I+ YR K
Sbjct: 440 --GDTVIGNKTSTE-------KMQSLQDDETVAKLKVDCISSNGYMIVVYRRK 457

BLAST of Carg13592 vs. TAIR 10
Match: AT1G03770.2 (RING 1B )

HSP 1 Score: 323.6 bits (828), Expect = 2.9e-88
Identity = 226/488 (46.31%), Postives = 302/488 (61.89%), Query Frame = 0

Query: 28  KQCRAERQPNTEVEEEGEEEQKKPKKEAEREEKQHKQLEEGTGARDSEESQSSSSEEKPE 87
           K   A  + + ++    E E+  P +  E+EE   K  E+     + ++ +    E + E
Sbjct: 5   KSFSAAEEEDDQLGRNSEAERFNP-EAVEKEEDPDKMDEKDESGDEEDDVKRDQVEAEDE 64

Query: 88  YFLLNPSTSFSLYDCHFNGAVDLMCGKFIFVELSGIRKDVQCPICLGIIKKTRTVMECLH 147
             L     S S      + A +L   +++ V+L+ I KDVQC ICLGII+KTRTVMECLH
Sbjct: 65  EALGEEEVSDSKERSQSSSAGELSESEYMVVDLADICKDVQCSICLGIIRKTRTVMECLH 124

Query: 148 RFCRECIDKSMRLGNNECPACRTHCASRRSLRDDPNYDALIAALYPDIDKYEEEELTFHE 207
           RFCRECIDKSMRLGNNECP CR HCASRRSLRDDPN+DALIAAL+ +IDK+EEEEL F +
Sbjct: 125 RFCRECIDKSMRLGNNECPTCRKHCASRRSLRDDPNFDALIAALFKNIDKFEEEELNFRQ 184

Query: 208 EERTRNKQIQESIAQTFQRQSEALSKKRMLGKDMPGMILTRSRRNHRNVHLRRQNGRGDE 267
           ++  RNKQIQ SIAQ  QRQS+AL K++ +GK     IL+RSRR+      RR+N R  E
Sbjct: 185 DDEARNKQIQASIAQVSQRQSKALVKRKSVGKGT--AILSRSRRSGGGSR-RRRNCRNIE 244

Query: 268 LLGYEDNEDEDDNSEGKDSSSADERCSKVRQRRKKRHSTVRSFQPSSSIASVDYGCTDGC 327
               E N+D+D N  GKDSSS DE C +   +RKKR +T    QPSSS A+ +  C    
Sbjct: 245 QDTSEANDDDDQNKRGKDSSS-DEPCER---QRKKRSAT----QPSSSNANNNDNCAGNG 304

Query: 328 AESDLDMSRENRTVSPGLVLNSEMLGWGRGGVRSNTRYGNAGGLSNKGS---RSGRLMKL 387
            E      R++R +SP LV NSE++ WGRGG RSNTR GN    +N+G+   R+ RL +L
Sbjct: 305 TEQ--THQRDSRVISPVLVWNSELIAWGRGGTRSNTRQGN----NNQGAISKRNARLKRL 364

Query: 388 VNYLRGLEENNNELDVHILLISVDSESTPSLKQPHLQCRPSLTVKHLRE------YVSRK 447
           V YL  LE N+ ELD+H+ L+S+D+E   +L +P+L  RP+L VK LRE      YV+R 
Sbjct: 365 VEYLGSLEGNSVELDIHLKLVSLDTEGLLNLHEPYLCFRPTLLVKQLREVSSLPLYVARH 424

Query: 448 TPLPADDVELLSVKDRYGTSDAQSTPSSSIFNPLKYELQILGGEETLAGLQADCTYSREH 507
             L A++VELL  KD  G +   +  S+        ++Q L  +ET+A L+ DC  S  +
Sbjct: 425 LKLKAEEVELLVSKD--GDTVIGNKTSTE-------KMQSLQDDETVAKLKVDCISSNGY 465

BLAST of Carg13592 vs. TAIR 10
Match: AT1G03770.3 (RING 1B )

HSP 1 Score: 321.6 bits (823), Expect = 1.1e-87
Identity = 225/479 (46.97%), Postives = 297/479 (62.00%), Query Frame = 0

Query: 37  NTEVEEEGEEEQKKPKKEAEREEKQHKQLEEGTGARDSEESQSSSSEEKPEYFLLNPSTS 96
           N+E E    E  +K +   + +EK     EE    RD  E++   +  + E       +S
Sbjct: 20  NSEAERFNPEAVEKEEDPDKMDEKDESGDEEDDVKRDQVEAEDEEALGEEEDSKERSQSS 79

Query: 97  FSLYDCHFNGAVDLMCGKFIFVELSGIRKDVQCPICLGIIKKTRTVMECLHRFCRECIDK 156
                     A +L   +++ V+L+ I KDVQC ICLGII+KTRTVMECLHRFCRECIDK
Sbjct: 80  ---------SAGELSESEYMVVDLADICKDVQCSICLGIIRKTRTVMECLHRFCRECIDK 139

Query: 157 SMRLGNNECPACRTHCASRRSLRDDPNYDALIAALYPDIDKYEEEELTFHEEERTRNKQI 216
           SMRLGNNECP CR HCASRRSLRDDPN+DALIAAL+ +IDK+EEEEL F +++  RNKQI
Sbjct: 140 SMRLGNNECPTCRKHCASRRSLRDDPNFDALIAALFKNIDKFEEEELNFRQDDEARNKQI 199

Query: 217 QESIAQTFQRQSEALSKKRMLGKDMPGMILTRSRRNHRNVHLRRQNGRGDELLGYEDNED 276
           Q SIAQ  QRQS+AL K++ +GK     IL+RSRR+      RR+N R  E    E N+D
Sbjct: 200 QASIAQVSQRQSKALVKRKSVGKGT--AILSRSRRSGGGSR-RRRNCRNIEQDTSEANDD 259

Query: 277 EDDNSEGKDSSSADERCSKVRQRRKKRHSTVRSFQPSSSIASVDYGCTDGCAESDLDMSR 336
           +D N  GKDSSS DE C +   +RKKR +T    QPSSS A+ +  C     E      R
Sbjct: 260 DDQNKRGKDSSS-DEPCER---QRKKRSAT----QPSSSNANNNDNCAGNGTEQ--THQR 319

Query: 337 ENRTVSPGLVLNSEMLGWGRGGVRSNTRYGNAGGLSNKGS---RSGRLMKLVNYLRGLEE 396
           ++R +SP LV NSE++ WGRGG RSNTR GN    +N+G+   R+ RL +LV YL  LE 
Sbjct: 320 DSRVISPVLVWNSELIAWGRGGTRSNTRQGN----NNQGAISKRNARLKRLVEYLGSLEG 379

Query: 397 NNNELDVHILLISVDSESTPSLKQPHLQCRPSLTVKHLRE------YVSRKTPLPADDVE 456
           N+ ELD+H+ L+S+D+E   +L +P+L  RP+L VK LRE      YV+R   L A++VE
Sbjct: 380 NSVELDIHLKLVSLDTEGLLNLHEPYLCFRPTLLVKQLREVSSLPLYVARHLKLKAEEVE 439

Query: 457 LLSVKDRYGTSDAQSTPSSSIFNPLKYELQILGGEETLAGLQADCTYSREHLILGYRWK 507
           LL  KD  G +   +  S+        ++Q L  +ET+A L+ DC  S  ++I+ YR K
Sbjct: 440 LLVSKD--GDTVIGNKTSTE-------KMQSLQDDETVAKLKVDCISSNGYMIVVYRRK 463

BLAST of Carg13592 vs. TAIR 10
Match: AT5G44280.2 (RING 1A )

HSP 1 Score: 299.3 bits (765), Expect = 5.9e-81
Identity = 215/517 (41.59%), Postives = 314/517 (60.74%), Query Frame = 0

Query: 10  DSLVGEELSDEQFSHQDAKQCRAERQPN-TEVEEEGEEEQKKPKKEAEREEKQHKQLEEG 69
           D    E+  DE+  H +A++ +   +PN  E +++G++ ++  ++E E EE +  + EE 
Sbjct: 36  DETKEEKEGDEEVKHDEAEEDQEVVKPNDAEEDDDGDDAEEDEEEEVEAEEDEEAE-EEE 95

Query: 70  TGARDSEESQSSSSEEKPEYFLLNPSTSFSLYDCHFNGAVDLMCGKFIFVELSGIRKDVQ 129
               + EE +  S E  P     + S                   +F+ ++L  IRKDVQ
Sbjct: 96  EEEEEEEEEEEDSKERSPSSISGDQS-------------------EFMEIDLGEIRKDVQ 155

Query: 130 CPICLGIIKKTRTVMECLHRFCRECIDKSMR----LGNNECPACRTHCASRRSLRDDPNY 189
           CPICL  +K  R  +   ++  ++C   S +      NNECPACR HCASRRSLRDDP +
Sbjct: 156 CPICLVGMKFLRLFIS-YYKENKDCDGMSPQFCATFKNNECPACRKHCASRRSLRDDPKF 215

Query: 190 DALIAALYPDIDKYEEEELTFHEEERTRNKQIQESIAQTFQRQSEALSKKRMLGKDMPGM 249
           DALIAAL+ +ID YEEEEL FHE+E  RNKQIQ SIAQ  QRQSEAL K+R LGK+   +
Sbjct: 216 DALIAALFTNIDSYEEEELAFHEDEMARNKQIQASIAQISQRQSEALVKRRSLGKEAAVL 275

Query: 250 -----ILTRSRRNHRNVHLRRQNGRGDELLGYEDNEDEDDNSEGKD-SSSADERCSKVRQ 309
                I + SRR   + ++ +QN        +ED++++D+N+ G+D  SS+DER ++VRQ
Sbjct: 276 MRSPRIASGSRRRRNSRNMEQQNASE----AHEDDDNDDNNNRGRDKDSSSDERGTEVRQ 335

Query: 310 RRKKRHSTVRSFQ-PSSSIASVDYGCTDGCAESDLDMSRENRTVSPGLVLNSEMLGWGRG 369
           +++++ ST RS Q PSSS A+ + G    CA++D ++ R+ + +SPGLV N E+L WGRG
Sbjct: 336 KKRRKRSTSRSTQHPSSSGANKNNG---NCADNDTEVYRDTKGISPGLVWNPEILAWGRG 395

Query: 370 GVRSNTRYG-NAGGLSNKGSRSGRLMKLVNYLR-GLEENNNELDVHILLISVDSESTPSL 429
           G RSNTR+G N  G S+K  R+ R+ KLV YLR  ++ ++ ELD+H+ L+S+D++  P L
Sbjct: 396 GTRSNTRHGNNTSGGSSKSVRNARVNKLVEYLRSSVDGSSVELDIHVKLVSLDTKCIPDL 455

Query: 430 KQPHLQCRPSLTVKHLREYVSRKTPLPADDVELLSVKDRYGTSDA-----QSTPSSSIFN 489
            QP+L CRP+L VK LRE+V+ +  L  ++VELL  + R G   A       TP+S+  +
Sbjct: 456 PQPYLCCRPTLLVKQLREFVALQIHLKTEEVELLVTRRRVGEDAAIENLPAVTPASAAAS 515

Query: 490 PLKYELQILGGEETLAGLQADCTYSRE-HLILGYRWK 507
             K E+  L   ETL+ L+ D   S E HLI+ YR K
Sbjct: 516 --KDEMLSLEDNETLSRLKIDFISSHEQHLIIAYRKK 522