Carg13574 (gene) Silver-seed gourd (SMH-JMG-627) v2

Overview
NameCarg13574
Typegene
OrganismCucurbita argyrosperma subsp. argyrosperma cv. SMH-JMG-627 (Silver-seed gourd (SMH-JMG-627) v2)
DescriptionO-methyltransferase, putative
LocationCarg_Chr02: 613250 .. 618177 (+)
RNA-Seq ExpressionCarg13574
SyntenyCarg13574
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGTCGGAGGAACTACTGTTTGAAGATCCGATGATTGTGTTCTTGTTTTTTGACAGTCTTAGGCACTGATGTTTTTATGACAGTTAATTGTGGAAAATTATTGTAGGTTGATGAAGATGAAATATATTGGAGACAGAGAAGGGGTGACGAAGAGTTAGAATGGTCACATCGTTCCACCCCTGTAATTTTACAGCTAGCTCAATGTTTTAGTGAGTATAGAGCAGTGTTTGAATGTTTTTTTTTTTTTTTTTTTTTTTAATGTTTTTGAGATGAAGTTGTAGAATTATGATTTTGTGGAATTCACACGTGGTTTTATATGCTCGCCATGTCTCAGTGTCACCCTGTTGTTTACAAATTAGCTTGTGGTAAATGTAGCTAATGCTATGGTTGGACCACGAACATGGATTGGAGGACTTTTCAATCGCGCAGGCAACAGAAAGAATGATAAGTTTGTTCAGTACTCGTTGAGTCCTCTTCAGGTAAAAATAGTGTTGGAAGGTCTATGGGTATTGTAATTGACTTGATGTTGTGTTATTTATCATTTTTTGTGTGTTGTGACTATTCTACATCACTCGTAGAATGGGCTAAACGTTGGTTTGTTAGTGTGAGGGAAGCTTTACCTCTTGAGTAGCTTTTGTCTTGGAAAGGCCATTGGTTTGTAATAGATCCTTGTGTTCCTGCCAACATCAGCATAGGTGAGTGATGAGAGCATCTTTACTCCCTCTAAGAGGTTATAGGTTTGAATCCCAACCCCCCATGTGTTGATGCAATGTTCTTAAAAAGAGAAAAAAAAAAAGAACAATAGATCTTTGTGTTCCCGAGAATGTCCGCTCATGTGCACCGCTAGTAGATATTGAACTCTTTGGGCTTTCCCTTTCGGGTATCCCCTTAAGGTTTTTAAAACGTGTATGCTAGAGAGAGGTTTGCACACCCTTATAAAGAATGTTTGGTTGTCCTCCCTAATCAATTTAAAACTTGTGATCCCCTTTTTATTGGATGAGAGCTTGTAAAAGCTAAAGTAAATGCAGTTTTATATAAAACCATTCTTCTAATCGCTTGTTCTAACATGGGATTCATGCAAACAGGAGGAAAGGTTTCAAAGGCTACAAGACCGAATTCCCATACCTTTTGATGAGACACAACTTGAACATCAAGTATTTTTCTTGTTTTAACTTTCAACAGCTGTGAGATGAGTTGTTGTTACTCTTGCTAATAATCGTTCATTGATGACAGGAAGCCCTGAAAGCATTGTGGGATGCTGCCTTTCCAAATATTGAGCTCAAAGGGATGGTATCTGAGCAATGGAAAGAAATGGGTTGGCAAGGACCTAATCCATCCACTGACTTTAGGTACACTCCACACCAACATCTCATATTAGTGTGTTCATATGTTGTATCAATACTTTATTTTTCATATTTAAAGTTAATTTTTTTCTCACAGTGAGCTGATTTCTTAGGGGTTGCGGTCTCATTTCGCTCGAAAATTTGCTGTTTTTCTCCAGGACGTTTCCGGTATGTCGTATCCTGCTGTTGTTCTGTGTCTCATTTCCCTAAATGCCTGAATTATCACTAATCACATACAAATTCAAAAAATGAAGGCATCTTTTCGTAGGTTGTTATTGAAGGAAGGTGGTAACCGAGCAACTTGGGAATATCCGTTTGCTGTTGCTGGCATAAACATTTCATTTATGTTGATTCAAATGTTGGATTTAAACTCAGGTGTGTGTCTGATGTTTTCTACTGAACGCTCCTATTTGTTTGGAGCTCTGCTTTTCTAGATGAAGCAATTAGAGGCTTAACTAACATTGGGCCAATATGCGTACCCATATTTAGACACTGAGTCGTCAATTTTGTGTTATTGTTCTTCATCTCACTTCGATCGGTCTAGATGTGATCGGTCTAGATGTGATCGAAGCAAGTTCAAATGCCTGAATAGCATACGTATCGAAGTCGATACAATTGCCTCGATAAACAATTTTTATGGGGTATAGGCCTTCCAAAATGTAATGGAAATGTGCTAAGGTTTCCTTAGGCCAAATAGAGATGGGCTCTCGGGCGCAACTTCTTGATGGGGCATAGATATTAGAAAATAGCCTGTGTAATCCTACATCAATTAGAGAGGAGAACGAAACATTCCTTATAAGGATATGGAAACCTCTCCCTAACAGATGCGTTTTACAACTATGAAGCTGACAGTGATACATAACAAACTAAAACGGACAATATCTGCTAGCGGTGAGCTTTAGCTGTTACAAATGGTATCAGAGTTAAACACTAGACGGTGTGCCAGCGAGGACACTGGGATTCCAAGGGAGTAGATTGTGAGATCTCACATCGATTGGAGGGGGAAACGAAACATTCCTTATAAGGTTTCGGTGATACGTAATGCATTAAAGCAGACAATATCGACTAGTGGTGAGATTTCGAAATTTGTTATGATAACTAATAGAATCTTGTCATCTCGTTACAGAAAGGCCAAGGTGCCTTCCGGGACTCAATTTTGTGAGACTATTAGGAGGTACTGGGTTTGTTTCATATTGATTCGTACACATATTTTCATTGCTAAACTTGTACTTCGTTTCGTTTCATCTCGTTTTCGTTGGTCGTGACAGAAAACGAAGAAGCATTCGACATACTATATTGCGTAGCGTTCAAAATGATGGATGCTCAGTGGGTTGCCATGCATGCTTCGTACATGGAGTTCAATGTATGCTCCTCCTCTTTCTCTCCTAAAACTTGAATCCATCAAGAATTCGCTAAGTGTTGGAAGTTTTTGATTGTTGTAACTATCAGGAGGTGCTGCAAGAAACCAGAGCGCAGCTGGAAAGAGAGCTATCTCTGGATGATGTTCACAGAATAGAAGATCTACCATCTTATAATCTCTTATATCTCTCTAACTTATCTTTTACGTCTCTCTCTACTAACTTTTCGTAGGGATTGTTATTATTCTTTTTTGAATCCATAAATACAAGCATTAGGGTACGATGTTCTAAATGTTCCAACTAGACATATGATATAAAGCCAACAAAATTTAACAAACATTCATTCTTCGTCTACAAAATTGAATAAGTTTGGCCATCGACCTCAATTTCATGAGTTAGAAGCCGATTTAATTTCATGAGAAATTATCATATTAATTTCATCACTTCTGCTTAAATGGTGAGAAGTTACTTGTTAAAATGATTCTAACTTATTCTTAGTGGACACTTGTCAATCATGGTCATAACTTTTGGAGTGGACATTCCAGAGTGGGAAGAACTTGTCAATAATCATGGCCACTGAATAGAATTTAGTGGGTATTAGAAGAGTTTGTTTTAGGGAAGACTATAAATACTCACGAATTAATACGTAAAAATTCCATATTTTTTTATGGATAAAATTGAGCTCTCGAGACTATCTTTGTCATTCCTAAGTTTTCAAGGAATTATTATGATAGATTGCATCTTATTTTCGTAGGTTGCATTTGAACCATTCAATCAAATTTTTATTTTAGTGACTTAATTTAGGATAAGGTTTCAAATCATGCTTTTTAATATTTAATCTTACTAAAATACGTTATATATTTGTGTATAAATATGAAAAGGGAAATGACATAAATACCCTTTGGTTTTGGACAGAAAAAGGCAAGATAAGGCCTTTGAAAAAGCAACCATAACAAATCATTATAAATATTGTCACCCTTTGTGAATGTCACCCCCATTACACCAACTCCATTTTATAATTCTCAACTATCTCTCTAAAACAGAATAAAACCAGAGAGATTGAATCGGAGAGACAGAGAGAGATGGCAACCAAAGAAACAGAGGAAACAGAGGAGCTGCTTAAAGGCCAAGCAGAGATATGGAAATATATGCTCTGTTTTGCTGATTCAATGGCCTTAAAATGCGCCGTCGAGCTTCACTTGGCTGATATCATCAACTCTCATGGCTCTCCCATATCGCTATCTCAGATTGCTTCCTCCATTGTCGCCTCCGATCCCTTAGCCACCCCGGAGATCTCCTACCTCAGTCGAATCATGAGACTGTTGGTCCGTCGGAACATCTTCGCTGTTCATCACTCATCAGACGGCGGAGAGCCACTTTATGGACTCACCCACTCCTCCAAATGGCTCCTACGTGACGCCGAGCTCACCCTCGCTCCAATGGTGCTAGCGGAGCTTCACAAATGGATGGTCGACCCATGGCTCTGTTTCTCCCGAGGCGTTAAAGAAGGCGGCGACCAATTCCAAAAAGCCAACGGCCTTGACATTTGGAGCTTCGCTTCCACAAACCCCCAATTCAACCAATTGTTCAACAACGCCATGGCTTCCACTTCCAAAGTCATCATGAAAGCGATTCTCTCCGTTTACCAAGATGGGTTCAGCTCGATTGAGTCACTAACCGACGTCGGCGGTGGAATCGGCGGCGTTATCTCAGAAATCGTCAAAGCATTCCCCCACATCAAGGGCATGAACTTCGACCTGCCGCACGTAATCAGCACAGCTCCGCTGTATCAAGGAGTGACCCACATCGGAGGGGACATGTTCGAAGCCATTCCCAAGGCCGATGCCGTGTTTATGAAGGTGATTGATTTGATTGCTTCCTAAAGTTTGAATTTTTTTGATGTAATTTGTGTTGAAATGGGTGATTACAGTGGATATTGCACGATTGGGATGACAAGGAGTGCGTGAGGATTCTTAAGAACTGCAAGAAAGCGATACCGGAGAAAACAGGGAAAGTGATTCTAGTAGAAGTGATTTTGAAGGCGGATGGAGATGGGGCATTTGATGATACTCGATTCTATTTTGATCTGTTAATGTTGGCGCATACCAATGGAAGAGAGAGGACAGAGAAGGAGTGGAAGAACATATTGGAAGAAGCTGGGTTTCCTCGATACAGAGTTATTGCTCTTCCTGCTATAACTTCCATTATTGAAGCTTATCCATCCTGA

mRNA sequence

ATGTCGGAGGAACTACTGTTTGAAGATCCGATGATTGTTGATGAAGATGAAATATATTGGAGACAGAGAAGGGGTGACGAAGAGTTAGAATGGTCACATCGTTCCACCCCTGTAATTTTACAGCTAGCTCAATGTTTTACTAATGCTATGGTTGGACCACGAACATGGATTGGAGGACTTTTCAATCGCGCAGGCAACAGAAAGAATGATAAGTTTGTTCAGTACTCGTTGAGTCCTCTTCAGGAGGAAAGGTTTCAAAGGCTACAAGACCGAATTCCCATACCTTTTGATGAGACACAACTTGAACATCAAGAAGCCCTGAAAGCATTGTGGGATGCTGCCTTTCCAAATATTGAGCTCAAAGGGATGGTATCTGAGCAATGGAAAGAAATGGGTTGGCAAGGACCTAATCCATCCACTGACTTTAGGGGTTGCGGTCTCATTTCGCTCGAAAATTTGCTGTTTTTCTCCAGGACGTTTCCGGCATCTTTTCGTAGGTTGTTATTGAAGGAAGGTGGTAACCGAGCAACTTGGGAATATCCGTTTGCTGTTGCTGGCATAAACATTTCATTTATGTTGATTCAAATGTTGGATTTAAACTCAGAAAGGCCAAGGTGCCTTCCGGGACTCAATTTTGTGAGACTATTAGGAGAAAACGAAGAAGCATTCGACATACTATATTGCGTAGCGTTCAAAATGATGGATGCTCAGTGGGTTGCCATGCATGCTTCGTACATGGAGTTCAATGAGGTGCTGCAAGAAACCAGAGCGCAGCTGGAAAGAGAGCTATCTCTGGATGATAATAAAACCAGAGAGATTGAATCGGAGAGACAGAGAGAGATGGCAACCAAAGAAACAGAGGAAACAGAGGAGCTGCTTAAAGGCCAAGCAGAGATATGGAAATATATGCTCTGTTTTGCTGATTCAATGGCCTTAAAATGCGCCGTCGAGCTTCACTTGGCTGATATCATCAACTCTCATGGCTCTCCCATATCGCTATCTCAGATTGCTTCCTCCATTGTCGCCTCCGATCCCTTAGCCACCCCGGAGATCTCCTACCTCAGTCGAATCATGAGACTGTTGGTCCGTCGGAACATCTTCGCTGTTCATCACTCATCAGACGGCGGAGAGCCACTTTATGGACTCACCCACTCCTCCAAATGGCTCCTACGTGACGCCGAGCTCACCCTCGCTCCAATGGTGCTAGCGGAGCTTCACAAATGGATGGTCGACCCATGGCTCTGTTTCTCCCGAGGCGTTAAAGAAGGCGGCGACCAATTCCAAAAAGCCAACGGCCTTGACATTTGGAGCTTCGCTTCCACAAACCCCCAATTCAACCAATTGTTCAACAACGCCATGGCTTCCACTTCCAAAGTCATCATGAAAGCGATTCTCTCCGTTTACCAAGATGGGTTCAGCTCGATTGAGTCACTAACCGACGTCGGCGGTGGAATCGGCGGCGTTATCTCAGAAATCGTCAAAGCATTCCCCCACATCAAGGGCATGAACTTCGACCTGCCGCACGTAATCAGCACAGCTCCGCTGTATCAAGGAGTGACCCACATCGGAGGGGACATGTTCGAAGCCATTCCCAAGGCCGATGCCGTGTTTATGAAGTGGATATTGCACGATTGGGATGACAAGGAGTGCGTGAGGATTCTTAAGAACTGCAAGAAAGCGATACCGGAGAAAACAGGGAAAGTGATTCTAGTAGAAGTGATTTTGAAGGCGGATGGAGATGGGGCATTTGATGATACTCGATTCTATTTTGATCTGTTAATGTTGGCGCATACCAATGGAAGAGAGAGGACAGAGAAGGAGTGGAAGAACATATTGGAAGAAGCTGGGTTTCCTCGATACAGAGTTATTGCTCTTCCTGCTATAACTTCCATTATTGAAGCTTATCCATCCTGA

Coding sequence (CDS)

ATGTCGGAGGAACTACTGTTTGAAGATCCGATGATTGTTGATGAAGATGAAATATATTGGAGACAGAGAAGGGGTGACGAAGAGTTAGAATGGTCACATCGTTCCACCCCTGTAATTTTACAGCTAGCTCAATGTTTTACTAATGCTATGGTTGGACCACGAACATGGATTGGAGGACTTTTCAATCGCGCAGGCAACAGAAAGAATGATAAGTTTGTTCAGTACTCGTTGAGTCCTCTTCAGGAGGAAAGGTTTCAAAGGCTACAAGACCGAATTCCCATACCTTTTGATGAGACACAACTTGAACATCAAGAAGCCCTGAAAGCATTGTGGGATGCTGCCTTTCCAAATATTGAGCTCAAAGGGATGGTATCTGAGCAATGGAAAGAAATGGGTTGGCAAGGACCTAATCCATCCACTGACTTTAGGGGTTGCGGTCTCATTTCGCTCGAAAATTTGCTGTTTTTCTCCAGGACGTTTCCGGCATCTTTTCGTAGGTTGTTATTGAAGGAAGGTGGTAACCGAGCAACTTGGGAATATCCGTTTGCTGTTGCTGGCATAAACATTTCATTTATGTTGATTCAAATGTTGGATTTAAACTCAGAAAGGCCAAGGTGCCTTCCGGGACTCAATTTTGTGAGACTATTAGGAGAAAACGAAGAAGCATTCGACATACTATATTGCGTAGCGTTCAAAATGATGGATGCTCAGTGGGTTGCCATGCATGCTTCGTACATGGAGTTCAATGAGGTGCTGCAAGAAACCAGAGCGCAGCTGGAAAGAGAGCTATCTCTGGATGATAATAAAACCAGAGAGATTGAATCGGAGAGACAGAGAGAGATGGCAACCAAAGAAACAGAGGAAACAGAGGAGCTGCTTAAAGGCCAAGCAGAGATATGGAAATATATGCTCTGTTTTGCTGATTCAATGGCCTTAAAATGCGCCGTCGAGCTTCACTTGGCTGATATCATCAACTCTCATGGCTCTCCCATATCGCTATCTCAGATTGCTTCCTCCATTGTCGCCTCCGATCCCTTAGCCACCCCGGAGATCTCCTACCTCAGTCGAATCATGAGACTGTTGGTCCGTCGGAACATCTTCGCTGTTCATCACTCATCAGACGGCGGAGAGCCACTTTATGGACTCACCCACTCCTCCAAATGGCTCCTACGTGACGCCGAGCTCACCCTCGCTCCAATGGTGCTAGCGGAGCTTCACAAATGGATGGTCGACCCATGGCTCTGTTTCTCCCGAGGCGTTAAAGAAGGCGGCGACCAATTCCAAAAAGCCAACGGCCTTGACATTTGGAGCTTCGCTTCCACAAACCCCCAATTCAACCAATTGTTCAACAACGCCATGGCTTCCACTTCCAAAGTCATCATGAAAGCGATTCTCTCCGTTTACCAAGATGGGTTCAGCTCGATTGAGTCACTAACCGACGTCGGCGGTGGAATCGGCGGCGTTATCTCAGAAATCGTCAAAGCATTCCCCCACATCAAGGGCATGAACTTCGACCTGCCGCACGTAATCAGCACAGCTCCGCTGTATCAAGGAGTGACCCACATCGGAGGGGACATGTTCGAAGCCATTCCCAAGGCCGATGCCGTGTTTATGAAGTGGATATTGCACGATTGGGATGACAAGGAGTGCGTGAGGATTCTTAAGAACTGCAAGAAAGCGATACCGGAGAAAACAGGGAAAGTGATTCTAGTAGAAGTGATTTTGAAGGCGGATGGAGATGGGGCATTTGATGATACTCGATTCTATTTTGATCTGTTAATGTTGGCGCATACCAATGGAAGAGAGAGGACAGAGAAGGAGTGGAAGAACATATTGGAAGAAGCTGGGTTTCCTCGATACAGAGTTATTGCTCTTCCTGCTATAACTTCCATTATTGAAGCTTATCCATCCTGA

Protein sequence

MSEELLFEDPMIVDEDEIYWRQRRGDEELEWSHRSTPVILQLAQCFTNAMVGPRTWIGGLFNRAGNRKNDKFVQYSLSPLQEERFQRLQDRIPIPFDETQLEHQEALKALWDAAFPNIELKGMVSEQWKEMGWQGPNPSTDFRGCGLISLENLLFFSRTFPASFRRLLLKEGGNRATWEYPFAVAGINISFMLIQMLDLNSERPRCLPGLNFVRLLGENEEAFDILYCVAFKMMDAQWVAMHASYMEFNEVLQETRAQLERELSLDDNKTREIESERQREMATKETEETEELLKGQAEIWKYMLCFADSMALKCAVELHLADIINSHGSPISLSQIASSIVASDPLATPEISYLSRIMRLLVRRNIFAVHHSSDGGEPLYGLTHSSKWLLRDAELTLAPMVLAELHKWMVDPWLCFSRGVKEGGDQFQKANGLDIWSFASTNPQFNQLFNNAMASTSKVIMKAILSVYQDGFSSIESLTDVGGGIGGVISEIVKAFPHIKGMNFDLPHVISTAPLYQGVTHIGGDMFEAIPKADAVFMKWILHDWDDKECVRILKNCKKAIPEKTGKVILVEVILKADGDGAFDDTRFYFDLLMLAHTNGRERTEKEWKNILEEAGFPRYRVIALPAITSIIEAYPS
Homology
BLAST of Carg13574 vs. NCBI nr
Match: KAG7034926.1 (xanthohumol 4'-O-methyltransferase, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 1293.1 bits (3345), Expect = 0.0e+00
Identity = 637/637 (100.00%), Postives = 637/637 (100.00%), Query Frame = 0

Query: 1   MSEELLFEDPMIVDEDEIYWRQRRGDEELEWSHRSTPVILQLAQCFTNAMVGPRTWIGGL 60
           MSEELLFEDPMIVDEDEIYWRQRRGDEELEWSHRSTPVILQLAQCFTNAMVGPRTWIGGL
Sbjct: 1   MSEELLFEDPMIVDEDEIYWRQRRGDEELEWSHRSTPVILQLAQCFTNAMVGPRTWIGGL 60

Query: 61  FNRAGNRKNDKFVQYSLSPLQEERFQRLQDRIPIPFDETQLEHQEALKALWDAAFPNIEL 120
           FNRAGNRKNDKFVQYSLSPLQEERFQRLQDRIPIPFDETQLEHQEALKALWDAAFPNIEL
Sbjct: 61  FNRAGNRKNDKFVQYSLSPLQEERFQRLQDRIPIPFDETQLEHQEALKALWDAAFPNIEL 120

Query: 121 KGMVSEQWKEMGWQGPNPSTDFRGCGLISLENLLFFSRTFPASFRRLLLKEGGNRATWEY 180
           KGMVSEQWKEMGWQGPNPSTDFRGCGLISLENLLFFSRTFPASFRRLLLKEGGNRATWEY
Sbjct: 121 KGMVSEQWKEMGWQGPNPSTDFRGCGLISLENLLFFSRTFPASFRRLLLKEGGNRATWEY 180

Query: 181 PFAVAGINISFMLIQMLDLNSERPRCLPGLNFVRLLGENEEAFDILYCVAFKMMDAQWVA 240
           PFAVAGINISFMLIQMLDLNSERPRCLPGLNFVRLLGENEEAFDILYCVAFKMMDAQWVA
Sbjct: 181 PFAVAGINISFMLIQMLDLNSERPRCLPGLNFVRLLGENEEAFDILYCVAFKMMDAQWVA 240

Query: 241 MHASYMEFNEVLQETRAQLERELSLDDNKTREIESERQREMATKETEETEELLKGQAEIW 300
           MHASYMEFNEVLQETRAQLERELSLDDNKTREIESERQREMATKETEETEELLKGQAEIW
Sbjct: 241 MHASYMEFNEVLQETRAQLERELSLDDNKTREIESERQREMATKETEETEELLKGQAEIW 300

Query: 301 KYMLCFADSMALKCAVELHLADIINSHGSPISLSQIASSIVASDPLATPEISYLSRIMRL 360
           KYMLCFADSMALKCAVELHLADIINSHGSPISLSQIASSIVASDPLATPEISYLSRIMRL
Sbjct: 301 KYMLCFADSMALKCAVELHLADIINSHGSPISLSQIASSIVASDPLATPEISYLSRIMRL 360

Query: 361 LVRRNIFAVHHSSDGGEPLYGLTHSSKWLLRDAELTLAPMVLAELHKWMVDPWLCFSRGV 420
           LVRRNIFAVHHSSDGGEPLYGLTHSSKWLLRDAELTLAPMVLAELHKWMVDPWLCFSRGV
Sbjct: 361 LVRRNIFAVHHSSDGGEPLYGLTHSSKWLLRDAELTLAPMVLAELHKWMVDPWLCFSRGV 420

Query: 421 KEGGDQFQKANGLDIWSFASTNPQFNQLFNNAMASTSKVIMKAILSVYQDGFSSIESLTD 480
           KEGGDQFQKANGLDIWSFASTNPQFNQLFNNAMASTSKVIMKAILSVYQDGFSSIESLTD
Sbjct: 421 KEGGDQFQKANGLDIWSFASTNPQFNQLFNNAMASTSKVIMKAILSVYQDGFSSIESLTD 480

Query: 481 VGGGIGGVISEIVKAFPHIKGMNFDLPHVISTAPLYQGVTHIGGDMFEAIPKADAVFMKW 540
           VGGGIGGVISEIVKAFPHIKGMNFDLPHVISTAPLYQGVTHIGGDMFEAIPKADAVFMKW
Sbjct: 481 VGGGIGGVISEIVKAFPHIKGMNFDLPHVISTAPLYQGVTHIGGDMFEAIPKADAVFMKW 540

Query: 541 ILHDWDDKECVRILKNCKKAIPEKTGKVILVEVILKADGDGAFDDTRFYFDLLMLAHTNG 600
           ILHDWDDKECVRILKNCKKAIPEKTGKVILVEVILKADGDGAFDDTRFYFDLLMLAHTNG
Sbjct: 541 ILHDWDDKECVRILKNCKKAIPEKTGKVILVEVILKADGDGAFDDTRFYFDLLMLAHTNG 600

Query: 601 RERTEKEWKNILEEAGFPRYRVIALPAITSIIEAYPS 638
           RERTEKEWKNILEEAGFPRYRVIALPAITSIIEAYPS
Sbjct: 601 RERTEKEWKNILEEAGFPRYRVIALPAITSIIEAYPS 637

BLAST of Carg13574 vs. NCBI nr
Match: KAG6604808.1 (Xanthohumol 4-O-methyltransferase, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 719.9 bits (1857), Expect = 1.9e-203
Identity = 356/357 (99.72%), Postives = 356/357 (99.72%), Query Frame = 0

Query: 281 MATKETEETEELLKGQAEIWKYMLCFADSMALKCAVELHLADIINSHGSPISLSQIASSI 340
           MATKETEETEELLKGQAEIWKYMLCFADSMALKCAVELHLADIINSHGSPISLSQIASSI
Sbjct: 1   MATKETEETEELLKGQAEIWKYMLCFADSMALKCAVELHLADIINSHGSPISLSQIASSI 60

Query: 341 VASDPLATPEISYLSRIMRLLVRRNIFAVHHSSDGGEPLYGLTHSSKWLLRDAELTLAPM 400
           VASDPLATPEISYLSRIMRLLVRRNIFAVHHSSDGGEPLYGLTHSSKWLLRDAELTLAPM
Sbjct: 61  VASDPLATPEISYLSRIMRLLVRRNIFAVHHSSDGGEPLYGLTHSSKWLLRDAELTLAPM 120

Query: 401 VLAELHKWMVDPWLCFSRGVKEGGDQFQKANGLDIWSFASTNPQFNQLFNNAMASTSKVI 460
           VLAELHKWMVDPWLCFSRGVKEGGDQFQKANGLDIWSFASTNPQFNQLFNNAMASTSKVI
Sbjct: 121 VLAELHKWMVDPWLCFSRGVKEGGDQFQKANGLDIWSFASTNPQFNQLFNNAMASTSKVI 180

Query: 461 MKAILSVYQDGFSSIESLTDVGGGIGGVISEIVKAFPHIKGMNFDLPHVISTAPLYQGVT 520
           MKAILSVYQDGFSSIESLTDVGGGIGGVISEIVKAFPHIKGMNFDLPHVISTAP YQGVT
Sbjct: 181 MKAILSVYQDGFSSIESLTDVGGGIGGVISEIVKAFPHIKGMNFDLPHVISTAPPYQGVT 240

Query: 521 HIGGDMFEAIPKADAVFMKWILHDWDDKECVRILKNCKKAIPEKTGKVILVEVILKADGD 580
           HIGGDMFEAIPKADAVFMKWILHDWDDKECVRILKNCKKAIPEKTGKVILVEVILKADGD
Sbjct: 241 HIGGDMFEAIPKADAVFMKWILHDWDDKECVRILKNCKKAIPEKTGKVILVEVILKADGD 300

Query: 581 GAFDDTRFYFDLLMLAHTNGRERTEKEWKNILEEAGFPRYRVIALPAITSIIEAYPS 638
           GAFDDTRFYFDLLMLAHTNGRERTEKEWKNILEEAGFPRYRVIALPAITSIIEAYPS
Sbjct: 301 GAFDDTRFYFDLLMLAHTNGRERTEKEWKNILEEAGFPRYRVIALPAITSIIEAYPS 357

BLAST of Carg13574 vs. NCBI nr
Match: XP_022946951.1 (xanthohumol 4'-O-methyltransferase-like [Cucurbita moschata])

HSP 1 Score: 711.8 bits (1836), Expect = 5.2e-201
Identity = 351/355 (98.87%), Postives = 354/355 (99.72%), Query Frame = 0

Query: 283 TKETEETEELLKGQAEIWKYMLCFADSMALKCAVELHLADIINSHGSPISLSQIASSIVA 342
           T+ETEETEELLKGQAEIWKYMLCFADSMALKCAVELHLADIINSHGSPISLSQIASSIVA
Sbjct: 6   TEETEETEELLKGQAEIWKYMLCFADSMALKCAVELHLADIINSHGSPISLSQIASSIVA 65

Query: 343 SDPLATPEISYLSRIMRLLVRRNIFAVHHSSDGGEPLYGLTHSSKWLLRDAELTLAPMVL 402
           SDPLATPEISYLSRIMRLLVRRNIFAVHHSSDGGEPLYGLTHSSKWLLRDAELTLAPMVL
Sbjct: 66  SDPLATPEISYLSRIMRLLVRRNIFAVHHSSDGGEPLYGLTHSSKWLLRDAELTLAPMVL 125

Query: 403 AELHKWMVDPWLCFSRGVKEGGDQFQKANGLDIWSFASTNPQFNQLFNNAMASTSKVIMK 462
           AELHKWMVDPWLCFS+GVKEGGDQFQKANGLDIWSFASTNPQFNQLFNNAMASTSKVIMK
Sbjct: 126 AELHKWMVDPWLCFSQGVKEGGDQFQKANGLDIWSFASTNPQFNQLFNNAMASTSKVIMK 185

Query: 463 AILSVYQDGFSSIESLTDVGGGIGGVISEIVKAFPHIKGMNFDLPHVISTAPLYQGVTHI 522
           AILSVYQDGFSSIESLTDVGGGIGGVISEIVKAFPHIKG+NFDLPHVISTAP YQGVTHI
Sbjct: 186 AILSVYQDGFSSIESLTDVGGGIGGVISEIVKAFPHIKGINFDLPHVISTAPPYQGVTHI 245

Query: 523 GGDMFEAIPKADAVFMKWILHDWDDKECVRILKNCKKAIPEKTGKVILVEVILKADGDGA 582
           GGDMFEAIPKADAVFMKWILHDWDDKECVRILKNCKKAIPEKTGKVILVEVILKADGDGA
Sbjct: 246 GGDMFEAIPKADAVFMKWILHDWDDKECVRILKNCKKAIPEKTGKVILVEVILKADGDGA 305

Query: 583 FDDTRFYFDLLMLAHTNGRERTEKEWKNILEEAGFPRYRVIALPAITSIIEAYPS 638
           FDDTRFYFDLLMLAHTNGRERTEKEWKNILEEAGFPRYRVIALPAITSIIEAYPS
Sbjct: 306 FDDTRFYFDLLMLAHTNGRERTEKEWKNILEEAGFPRYRVIALPAITSIIEAYPS 360

BLAST of Carg13574 vs. NCBI nr
Match: XP_023532623.1 (xanthohumol 4'-O-methyltransferase-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 699.9 bits (1805), Expect = 2.0e-197
Identity = 346/357 (96.92%), Postives = 350/357 (98.04%), Query Frame = 0

Query: 281 MATKETEETEELLKGQAEIWKYMLCFADSMALKCAVELHLADIINSHGSPISLSQIASSI 340
           MATKETEETEELLKGQAEIWKYMLCFADSMALKCAVELHLADIINSHGSPISLSQIASSI
Sbjct: 1   MATKETEETEELLKGQAEIWKYMLCFADSMALKCAVELHLADIINSHGSPISLSQIASSI 60

Query: 341 VASDPLATPEISYLSRIMRLLVRRNIFAVHHSSDGGEPLYGLTHSSKWLLRDAELTLAPM 400
           VASDPLATPEISYLSRIMRLLVRRNIFAVHHSSDGGEPLYGLTHSSKWLLRDAELTLAPM
Sbjct: 61  VASDPLATPEISYLSRIMRLLVRRNIFAVHHSSDGGEPLYGLTHSSKWLLRDAELTLAPM 120

Query: 401 VLAELHKWMVDPWLCFSRGVKEGGDQFQKANGLDIWSFASTNPQFNQLFNNAMASTSKVI 460
           VLAELHKW+VDPWLCFS+GVKEGGDQFQKAN LDIWSFAS NPQFNQLFNNAMASTSKVI
Sbjct: 121 VLAELHKWLVDPWLCFSQGVKEGGDQFQKANALDIWSFASINPQFNQLFNNAMASTSKVI 180

Query: 461 MKAILSVYQDGFSSIESLTDVGGGIGGVISEIVKAFPHIKGMNFDLPHVISTAPLYQGVT 520
           MKAILSVYQDG SSIESLTDVGGGIGGVISEIVKAFPHIKG+N+DLPHVISTAP YQGVT
Sbjct: 181 MKAILSVYQDGLSSIESLTDVGGGIGGVISEIVKAFPHIKGINYDLPHVISTAPPYQGVT 240

Query: 521 HIGGDMFEAIPKADAVFMKWILHDWDDKECVRILKNCKKAIPEKTGKVILVEVILKADGD 580
           HIGGDMFEAIPKADAVFMKWILHDWDDKECVRILKNCKKAIPEKTGKVILVEVILKADG 
Sbjct: 241 HIGGDMFEAIPKADAVFMKWILHDWDDKECVRILKNCKKAIPEKTGKVILVEVILKADGA 300

Query: 581 GAFDDTRFYFDLLMLAHTNGRERTEKEWKNILEEAGFPRYRVIALPAITSIIEAYPS 638
             FDDTRFYFDLLMLAHTNGRERTEKEWKNILEEAGFPRYRVIALPAITSIIEAYPS
Sbjct: 301 DPFDDTRFYFDLLMLAHTNGRERTEKEWKNILEEAGFPRYRVIALPAITSIIEAYPS 357

BLAST of Carg13574 vs. NCBI nr
Match: XP_022970718.1 (xanthohumol 4'-O-methyltransferase-like [Cucurbita maxima])

HSP 1 Score: 699.9 bits (1805), Expect = 2.0e-197
Identity = 343/357 (96.08%), Postives = 351/357 (98.32%), Query Frame = 0

Query: 281 MATKETEETEELLKGQAEIWKYMLCFADSMALKCAVELHLADIINSHGSPISLSQIASSI 340
           MATKETEETEELLKGQAEIWKYMLCFADSMALKCAVELHLADIINSH SP+SLSQIASSI
Sbjct: 1   MATKETEETEELLKGQAEIWKYMLCFADSMALKCAVELHLADIINSHASPLSLSQIASSI 60

Query: 341 VASDPLATPEISYLSRIMRLLVRRNIFAVHHSSDGGEPLYGLTHSSKWLLRDAELTLAPM 400
           VASDPLATPEISYL+RIMRLLVRRNIFAVHHS DGGEPLYGLTHSSKWLLRDAE TLAPM
Sbjct: 61  VASDPLATPEISYLTRIMRLLVRRNIFAVHHSPDGGEPLYGLTHSSKWLLRDAEHTLAPM 120

Query: 401 VLAELHKWMVDPWLCFSRGVKEGGDQFQKANGLDIWSFASTNPQFNQLFNNAMASTSKVI 460
           VLAELHKWMVDPWLCFS+GVKEGGDQFQKANGLDIWSFAS NPQFNQLFNNAMASTSKVI
Sbjct: 121 VLAELHKWMVDPWLCFSQGVKEGGDQFQKANGLDIWSFASRNPQFNQLFNNAMASTSKVI 180

Query: 461 MKAILSVYQDGFSSIESLTDVGGGIGGVISEIVKAFPHIKGMNFDLPHVISTAPLYQGVT 520
           MKA+LSVYQDGFSSIESLTDVGGGIGGVISEIVKAFPHIKG+N+DLPHVISTAP YQGVT
Sbjct: 181 MKAVLSVYQDGFSSIESLTDVGGGIGGVISEIVKAFPHIKGINYDLPHVISTAPPYQGVT 240

Query: 521 HIGGDMFEAIPKADAVFMKWILHDWDDKECVRILKNCKKAIPEKTGKVILVEVILKADGD 580
           HIGGDMFEAIPKADAVFMKWILHDWDDKECVRIL+NCKKAIPEKTGKVILVEVILKADGD
Sbjct: 241 HIGGDMFEAIPKADAVFMKWILHDWDDKECVRILENCKKAIPEKTGKVILVEVILKADGD 300

Query: 581 GAFDDTRFYFDLLMLAHTNGRERTEKEWKNILEEAGFPRYRVIALPAITSIIEAYPS 638
           G FDDTRFYFDLLMLAHTNGRERTEKEWKNILEEAGFPRYRVIALPA+TSIIEAYPS
Sbjct: 301 GPFDDTRFYFDLLMLAHTNGRERTEKEWKNILEEAGFPRYRVIALPALTSIIEAYPS 357

BLAST of Carg13574 vs. ExPASy Swiss-Prot
Match: B0ZB56 (Xanthohumol 4-O-methyltransferase OS=Humulus lupulus OX=3486 GN=OMT2 PE=1 SV=1)

HSP 1 Score: 403.3 bits (1035), Expect = 5.1e-111
Identity = 196/362 (54.14%), Postives = 265/362 (73.20%), Query Frame = 0

Query: 281 MATKETEETEELLKGQAEIWKYMLCFADSMALKCAVELHLADIINSHGSPISLSQIASSI 340
           M     ++TE  L+G+A +WK +   AD M +KCA+EL + DI++SH +PI+L+QIASS+
Sbjct: 1   MELARNDQTEAALRGEANVWKSINGIADFMVMKCALELRIPDIVHSHSAPITLAQIASSV 60

Query: 341 VASDPLATPEISYLSRIMRLLVRRNIFAVHHSSDGGEPLYGLTHSSKWLLRDA----ELT 400
             S  L    +SYLSRIMRLLVRR IF+ H S DG E LYG THSS+ LL       ++T
Sbjct: 61  PDSPSL---NLSYLSRIMRLLVRRKIFSQHKSLDGEEVLYGPTHSSRLLLSKTTLPDQVT 120

Query: 401 LAPMVLAELHKWMVDPWLCFSRGVKEGGDQFQKAN-GLDIWSFASTNPQFNQLFNNAMAS 460
           LAP V    H ++  PW C +R VKEGG+ F+  + G  +W  +  NP+FN++FN+ MAS
Sbjct: 121 LAPFVAFMTHPYLSAPWSCLARCVKEGGNGFEMVHGGRQLWDLSPGNPEFNKVFNDGMAS 180

Query: 461 TSKVIMKAILSVYQDGFSSIESLTDVGGGIGGVISEIVKAFPHIKGMNFDLPHVISTAPL 520
           T+++   AILS Y+D F  I SL DVGG  GG IS IVK+ PHIKG+N+DLPHV++TAP 
Sbjct: 181 TARITTMAILSEYRDVFCGICSLVDVGGEFGGSISAIVKSHPHIKGINYDLPHVVATAPT 240

Query: 521 YQG-VTHIGGDMFEAIPKADAVFMKWILHDWDDKECVRILKNCKKAIPEKTGKVILVEVI 580
           Y G V+H+GG+MFE IP A AVFMKWILHDW D++CV+ILKNC++A+PEK GK+I+V+++
Sbjct: 241 YTGLVSHVGGNMFEWIPTAVAVFMKWILHDWADEDCVKILKNCRRAMPEKGGKIIIVDIV 300

Query: 581 LKADGDGAFDDTRFYFDLLMLAHTNGRERTEKEWKNILEEAGFPRYRVIALPAITSIIEA 637
           L+ +G+G FDD     D+ ++A T G+ERTEKEWK +LEE GFPRY+++ +PA+TS+IEA
Sbjct: 301 LEPEGNGLFDDAAVMLDIALMALTRGKERTEKEWKRVLEEGGFPRYQILKIPALTSVIEA 359

BLAST of Carg13574 vs. ExPASy Swiss-Prot
Match: Q6WUC2 ((R,S)-reticuline 7-O-methyltransferase OS=Papaver somniferum OX=3469 GN=7OMT PE=1 SV=1)

HSP 1 Score: 384.0 bits (985), Expect = 3.2e-105
Identity = 186/353 (52.69%), Postives = 247/353 (69.97%), Query Frame = 0

Query: 287 EETEELLKGQAEIWKYMLCFADSMALKCAVELHLADIINSHGSPISLSQIASSIVASDPL 346
           +  EE LKGQAEIW++M  F DSMALKCAVEL + DIINSHG P+++S+I  S+  + P 
Sbjct: 2   DTAEERLKGQAEIWEHMFAFVDSMALKCAVELGIPDIINSHGRPVTISEIVDSLKTNTPS 61

Query: 347 ATPEISYLSRIMRLLVRRNIFAVHHSSDGGEPLYGLTHSSKWLLRDAELTLAPMVLAELH 406
           ++P I YL+RIMRLLV + +F      +  + LY LT SSKWLL+D++  L+P+VL E +
Sbjct: 62  SSPNIDYLTRIMRLLVHKRLFTSELHQESNQLLYNLTRSSKWLLKDSKFNLSPLVLWETN 121

Query: 407 KWMVDPWLCFSRGVKEGGDQFQKANGLDIWSFASTNPQFNQLFNNAMASTSKVIMKAILS 466
             ++ PW    +  +E    F++A+G +IW  A  +P+FN   N AM  ++  I+  +L 
Sbjct: 122 PILLKPWQYLGKCAQEKSSPFERAHGCEIWDLALADPKFNNFLNGAMQCSTTTIINEMLL 181

Query: 467 VYQDGFSSIE-SLTDVGGGIGGVISEIVKAFPHIKGMNFDLPHVISTAPLYQGVTHIGGD 526
            Y+DGFS I  SL DVGGG G +I+EIVKA PHI+G+NFDLPHV++TA  + GV H+GGD
Sbjct: 182 EYKDGFSGIAGSLVDVGGGTGSIIAEIVKAHPHIQGINFDLPHVVATAAEFPGVKHVGGD 241

Query: 527 MFEAIPKADAVFMKWILHDWDDKECVRILKNCKKAI-PEKTGKVILVEVILKADGDGAFD 586
           MF  IP+ADAV MKWILHDW D++C  ILKNC +AI  +K GKVI+V+ +L+ DG+  FD
Sbjct: 242 MFVDIPEADAVIMKWILHDWSDEDCTIILKNCYRAIRKKKNGKVIIVDCVLRPDGNDLFD 301

Query: 587 DTRFYFDLLMLAHTN-GRERTEKEWKNILEEAGFPRYRVIALPAITSIIEAYP 637
                FD+LM+AHT  G+ERTE EWK +L  AGFPRY VI  PA   IIEA+P
Sbjct: 302 KMGLIFDVLMMAHTTAGKERTEAEWKILLNNAGFPRYNVIRTPAFPCIIEAFP 354

BLAST of Carg13574 vs. ExPASy Swiss-Prot
Match: B0ZB55 (Desmethylxanthohumol 6'-O-methyltransferase OS=Humulus lupulus OX=3486 GN=OMT1 PE=1 SV=2)

HSP 1 Score: 371.3 bits (952), Expect = 2.2e-101
Identity = 185/349 (53.01%), Postives = 251/349 (71.92%), Query Frame = 0

Query: 291 ELLKGQAEIWKYMLCFADSMALKCAVELHLADIINSHGSPISLSQIASSIVA-SDPLATP 350
           E L+GQ +IW+ M  F DSMALKCA+EL +ADII+SHG PI+LSQIAS I + S+   +P
Sbjct: 2   ESLRGQEQIWQLMFSFVDSMALKCAIELRIADIIHSHGKPITLSQIASGIRSNSNSSISP 61

Query: 351 EISYLSRIMRLLVRRNIFAVH-HSSDGGEPLYGLTHSSKWLLRDAELTLAPMVLAELHKW 410
            I YLSRIMR LVR+NIF  H   +D    LYGL+ SS+WLLRD + +LAPMVL + H  
Sbjct: 62  NIPYLSRIMRFLVRKNIFTEHQEDNDEVISLYGLSDSSRWLLRDFKSSLAPMVLMQTHPL 121

Query: 411 MVDPWLCFSRGVKEGGDQFQKANGLDIWSFASTNPQFNQLFNNAMASTSKVIMKAILSVY 470
            +  W      V+   + F+KA+G +IW F+S NP FN++FNNAMAS   + M A+LS Y
Sbjct: 122 SMAVWHFLEDYVRNSSNTFEKAHGCNIWEFSSANPDFNKIFNNAMASIVPIYMGAVLSSY 181

Query: 471 QDGFSSIE-SLTDVGGGIGGVISEIVKAFPHIKGMNFDLPHVISTAPLYQGVTHIGGDMF 530
           +DG   I+ ++ DVGGG GG ISE++K +P+IKG+NFDLPHVI+TAP   GVTHI GD+F
Sbjct: 182 KDGLGCIKGTVVDVGGGTGGSISELMKYYPNIKGINFDLPHVIATAPALDGVTHISGDIF 241

Query: 531 EAIPKADAVFMKWILHDWDDKECVRILKNCKKAIPE-KTGKVILVEVILKADGDGAFDDT 590
           E+IP ADAV MK +LH + D++CV++L+NC+KAI + K GK+I++E++L    +  FD+T
Sbjct: 242 ESIPSADAVLMKGVLHCFSDEKCVKVLRNCRKAITDKKNGKIIILEIVLDPTSNQIFDET 301

Query: 591 RFYFDLLMLAHTNGRERTEKEWKNILEEAGFPRYRVIALPAITSIIEAY 636
           R  +DLL+   + G+ERTE EWK +L EAGF   ++  +P I +IIEA+
Sbjct: 302 RMVYDLLIPLFSGGKERTELEWKRLLNEAGFTSIKITKIPIIPAIIEAF 350

BLAST of Carg13574 vs. ExPASy Swiss-Prot
Match: Q9LEL6 ((RS)-norcoclaurine 6-O-methyltransferase OS=Coptis japonica OX=3442 PE=1 SV=1)

HSP 1 Score: 291.2 bits (744), Expect = 2.8e-77
Identity = 148/351 (42.17%), Postives = 223/351 (63.53%), Query Frame = 0

Query: 287 EETEELLKGQAEIWKYMLCFADSMALKCAVELHLADIINSHGSPISLSQIASSIVASDPL 346
           E  ++ L  QA++W ++  FA+S+ LKCAV+L LA+II++ G+ ++LS+++S +    P 
Sbjct: 2   EVKKDNLSSQAKLWNFIYGFAESLVLKCAVQLDLANIIHNSGTSMTLSELSSRL----PS 61

Query: 347 ATPEISYLSRIMRLLVRRNIFAVHHSSDGGEPLYGLTHSSKWLLRDAELTLAPMVLAELH 406
                  L R+MR LV   +F    +S  GE  YGL   +K+L++  +  +   +LA   
Sbjct: 62  QPVNEDALYRVMRYLVHMKLFT--KASIDGELRYGLAPPAKYLVKGWDKCMVGSILAITD 121

Query: 407 KWMVDPWLCFSRGVK-EGGDQFQKANGLDIWSFASTNPQFNQLFNNAMASTSKVIMKAIL 466
           K  + PW     G+  E G  F+KA G +IW + + +P+ NQLFN AMA+ S++IM A++
Sbjct: 122 KDFMAPWHYLKDGLSGESGTAFEKALGTNIWGYMAEHPEKNQLFNEAMANDSRLIMSALV 181

Query: 467 SVYQDGFSSIESLTDVGGGIGGVISEIVKAFPHIKGMNFDLPHVISTAPLYQGVTHIGGD 526
               + F+ I +L DVGGG G  +  I  AFPHIK   +DLPHVI+ +P Y  V  + GD
Sbjct: 182 KECGNIFNGITTLVDVGGGTGTAVRNIANAFPHIKCTVYDLPHVIADSPGYSEVHCVAGD 241

Query: 527 MFEAIPKADAVFMKWILHDWDDKECVRILKNCKKAIPEKTGKVILVEVILKADGDGAFDD 586
           MF+ IPKADA+ MK ILHDWDDKEC+ ILK CK+A+P K GKVI+V+++L    +  +  
Sbjct: 242 MFKFIPKADAIMMKCILHDWDDKECIEILKRCKEAVPVKGGKVIIVDIVLNVQSEHPYTK 301

Query: 587 TRFYFDLLMLAHTNGRERTEKEWKNILEEAGFPRYRVIALPAITSIIEAYP 637
            R   DL M+ +T G+ERTE+EWK ++ +AG+  +++  + A+ S+IEAYP
Sbjct: 302 MRLTLDLDMMLNTGGKERTEEEWKKLIHDAGYKGHKITQITAVQSVIEAYP 346

BLAST of Carg13574 vs. ExPASy Swiss-Prot
Match: Q5C9L7 ((RS)-norcoclaurine 6-O-methyltransferase OS=Thalictrum flavum subsp. glaucum OX=150095 PE=1 SV=1)

HSP 1 Score: 287.0 bits (733), Expect = 5.4e-76
Identity = 145/350 (41.43%), Postives = 215/350 (61.43%), Query Frame = 0

Query: 290 EELLKGQAEIWKYMLCFADSMALKCAVELHLADIINSHGSPISLSQIASSIVASDPLATP 349
           +E L  QA++W ++  FADS+ LK AV+L LA+II++HGSP++LS+++  +    P    
Sbjct: 6   KENLSSQAKLWNFIYGFADSLVLKSAVQLDLANIIHNHGSPMTLSELSLHL----PSQPV 65

Query: 350 EISYLSRIMRLLVRRNIFAVHHSSDGGEPLYGLTHSSKWLLRDAELTLAPMVLAELHKWM 409
               L R++R LV   +F    SS  GE  YGL   +K+L++  +  +   +L    K  
Sbjct: 66  NQDALYRVLRYLVHMKLFT--KSSIDGELRYGLAPPAKFLVKGWDKCMLGAILTITDKDF 125

Query: 410 VDPWLCFSRGVKEGGD---QFQKANGLDIWSFASTNPQFNQLFNNAMASTSKVIMKAILS 469
           + PW     G+   G     F+KA G +IW + + +P+ NQLFN  MA+ +++IM A++ 
Sbjct: 126 MAPWHYLKEGILNDGSTSTAFEKALGTNIWDYMAEHPEKNQLFNEGMANDTRLIMSALVK 185

Query: 470 VYQDGFSSIESLTDVGGGIGGVISEIVKAFPHIKGMNFDLPHVISTAPLYQGVTHIGGDM 529
                F  I ++ DVGGG G  +  I KAFPHIK   +DLPHVI+ +P Y  +  I GDM
Sbjct: 186 ECSSMFDGITTIVDVGGGTGTAVRNIAKAFPHIKCTVYDLPHVIADSPGYTEINSIQGDM 245

Query: 530 FEAIPKADAVFMKWILHDWDDKECVRILKNCKKAIPEKTGKVILVEVILKADGDGAFDDT 589
           F+ IP ADA+ MK ILHDWDDKEC+ ILK CK A+P   GKVI++++IL    +  +   
Sbjct: 246 FKYIPNADAIMMKCILHDWDDKECIEILKRCKDAVPRDGGKVIIIDIILDVKSEHPYTKM 305

Query: 590 RFYFDLLMLAHTNGRERTEKEWKNILEEAGFPRYRVIALPAITSIIEAYP 637
           R   DL M+ +T G+ERTE+EWK ++ +AG+  Y++  + A+ S+IEAYP
Sbjct: 306 RLTLDLDMMLNTGGKERTEEEWKKLIHDAGYKGYKITHISAVQSVIEAYP 349

BLAST of Carg13574 vs. ExPASy TrEMBL
Match: A0A6J1G506 (xanthohumol 4'-O-methyltransferase-like OS=Cucurbita moschata OX=3662 GN=LOC111450978 PE=3 SV=1)

HSP 1 Score: 711.8 bits (1836), Expect = 2.5e-201
Identity = 351/355 (98.87%), Postives = 354/355 (99.72%), Query Frame = 0

Query: 283 TKETEETEELLKGQAEIWKYMLCFADSMALKCAVELHLADIINSHGSPISLSQIASSIVA 342
           T+ETEETEELLKGQAEIWKYMLCFADSMALKCAVELHLADIINSHGSPISLSQIASSIVA
Sbjct: 6   TEETEETEELLKGQAEIWKYMLCFADSMALKCAVELHLADIINSHGSPISLSQIASSIVA 65

Query: 343 SDPLATPEISYLSRIMRLLVRRNIFAVHHSSDGGEPLYGLTHSSKWLLRDAELTLAPMVL 402
           SDPLATPEISYLSRIMRLLVRRNIFAVHHSSDGGEPLYGLTHSSKWLLRDAELTLAPMVL
Sbjct: 66  SDPLATPEISYLSRIMRLLVRRNIFAVHHSSDGGEPLYGLTHSSKWLLRDAELTLAPMVL 125

Query: 403 AELHKWMVDPWLCFSRGVKEGGDQFQKANGLDIWSFASTNPQFNQLFNNAMASTSKVIMK 462
           AELHKWMVDPWLCFS+GVKEGGDQFQKANGLDIWSFASTNPQFNQLFNNAMASTSKVIMK
Sbjct: 126 AELHKWMVDPWLCFSQGVKEGGDQFQKANGLDIWSFASTNPQFNQLFNNAMASTSKVIMK 185

Query: 463 AILSVYQDGFSSIESLTDVGGGIGGVISEIVKAFPHIKGMNFDLPHVISTAPLYQGVTHI 522
           AILSVYQDGFSSIESLTDVGGGIGGVISEIVKAFPHIKG+NFDLPHVISTAP YQGVTHI
Sbjct: 186 AILSVYQDGFSSIESLTDVGGGIGGVISEIVKAFPHIKGINFDLPHVISTAPPYQGVTHI 245

Query: 523 GGDMFEAIPKADAVFMKWILHDWDDKECVRILKNCKKAIPEKTGKVILVEVILKADGDGA 582
           GGDMFEAIPKADAVFMKWILHDWDDKECVRILKNCKKAIPEKTGKVILVEVILKADGDGA
Sbjct: 246 GGDMFEAIPKADAVFMKWILHDWDDKECVRILKNCKKAIPEKTGKVILVEVILKADGDGA 305

Query: 583 FDDTRFYFDLLMLAHTNGRERTEKEWKNILEEAGFPRYRVIALPAITSIIEAYPS 638
           FDDTRFYFDLLMLAHTNGRERTEKEWKNILEEAGFPRYRVIALPAITSIIEAYPS
Sbjct: 306 FDDTRFYFDLLMLAHTNGRERTEKEWKNILEEAGFPRYRVIALPAITSIIEAYPS 360

BLAST of Carg13574 vs. ExPASy TrEMBL
Match: A0A6J1I4R1 (xanthohumol 4'-O-methyltransferase-like OS=Cucurbita maxima OX=3661 GN=LOC111469616 PE=3 SV=1)

HSP 1 Score: 699.9 bits (1805), Expect = 9.8e-198
Identity = 343/357 (96.08%), Postives = 351/357 (98.32%), Query Frame = 0

Query: 281 MATKETEETEELLKGQAEIWKYMLCFADSMALKCAVELHLADIINSHGSPISLSQIASSI 340
           MATKETEETEELLKGQAEIWKYMLCFADSMALKCAVELHLADIINSH SP+SLSQIASSI
Sbjct: 1   MATKETEETEELLKGQAEIWKYMLCFADSMALKCAVELHLADIINSHASPLSLSQIASSI 60

Query: 341 VASDPLATPEISYLSRIMRLLVRRNIFAVHHSSDGGEPLYGLTHSSKWLLRDAELTLAPM 400
           VASDPLATPEISYL+RIMRLLVRRNIFAVHHS DGGEPLYGLTHSSKWLLRDAE TLAPM
Sbjct: 61  VASDPLATPEISYLTRIMRLLVRRNIFAVHHSPDGGEPLYGLTHSSKWLLRDAEHTLAPM 120

Query: 401 VLAELHKWMVDPWLCFSRGVKEGGDQFQKANGLDIWSFASTNPQFNQLFNNAMASTSKVI 460
           VLAELHKWMVDPWLCFS+GVKEGGDQFQKANGLDIWSFAS NPQFNQLFNNAMASTSKVI
Sbjct: 121 VLAELHKWMVDPWLCFSQGVKEGGDQFQKANGLDIWSFASRNPQFNQLFNNAMASTSKVI 180

Query: 461 MKAILSVYQDGFSSIESLTDVGGGIGGVISEIVKAFPHIKGMNFDLPHVISTAPLYQGVT 520
           MKA+LSVYQDGFSSIESLTDVGGGIGGVISEIVKAFPHIKG+N+DLPHVISTAP YQGVT
Sbjct: 181 MKAVLSVYQDGFSSIESLTDVGGGIGGVISEIVKAFPHIKGINYDLPHVISTAPPYQGVT 240

Query: 521 HIGGDMFEAIPKADAVFMKWILHDWDDKECVRILKNCKKAIPEKTGKVILVEVILKADGD 580
           HIGGDMFEAIPKADAVFMKWILHDWDDKECVRIL+NCKKAIPEKTGKVILVEVILKADGD
Sbjct: 241 HIGGDMFEAIPKADAVFMKWILHDWDDKECVRILENCKKAIPEKTGKVILVEVILKADGD 300

Query: 581 GAFDDTRFYFDLLMLAHTNGRERTEKEWKNILEEAGFPRYRVIALPAITSIIEAYPS 638
           G FDDTRFYFDLLMLAHTNGRERTEKEWKNILEEAGFPRYRVIALPA+TSIIEAYPS
Sbjct: 301 GPFDDTRFYFDLLMLAHTNGRERTEKEWKNILEEAGFPRYRVIALPALTSIIEAYPS 357

BLAST of Carg13574 vs. ExPASy TrEMBL
Match: A0A7J6HG98 (ELMO domain-containing protein OS=Cannabis sativa OX=3483 GN=G4B88_018487 PE=3 SV=1)

HSP 1 Score: 657.5 bits (1695), Expect = 5.6e-185
Identity = 362/672 (53.87%), Postives = 450/672 (66.96%), Query Frame = 0

Query: 13  VDEDEIYWRQRRGDEELEWSHRSTPVILQLAQCFTNAMVGPRTWIGGLFNRAGNRKNDKF 72
           VDEDE YWRQ++GDEELEWSH ST VI QLAQCFTN M GPR+WIGGLF+R+GN K +K+
Sbjct: 112 VDEDETYWRQKKGDEELEWSHDSTHVIAQLAQCFTNVMGGPRSWIGGLFSRSGN-KREKY 171

Query: 73  VQYSLSPLQEERFQRLQDRIPIPFDETQLEHQEALKALWDAAFPNIELKGMVSEQWKEMG 132
           + Y LSPLQE+R QRLQ+R+ +PFDET+ +HQEAL+ LW AAFP+I LKG++SEQWKEMG
Sbjct: 172 LDYRLSPLQEQRLQRLQERLQVPFDETRPDHQEALRTLWLAAFPDITLKGLISEQWKEMG 231

Query: 133 WQGPNPSTDFRGCGLISLENLLFFSRTFPASFRRLLLKEGGNRATWEYPFAVAGINISFM 192
           WQGPNPSTDFR      +E L     T+PASFRRLLLK+ G RATWEYPFAVAGIN+SFM
Sbjct: 232 WQGPNPSTDFRYNSFPHMECL-----TYPASFRRLLLKQDGKRATWEYPFAVAGINVSFM 291

Query: 193 LIQMLDLNSERPRCLPGLNFVRLLGENEEAFDILYCVAFKMMDAQWVAMHASYMEFNEVL 252
           LIQMLDL S +P+C+PG+NF++LLGE+EEAFD+LYC+AF+MMDAQW+AMHASYMEFN + 
Sbjct: 292 LIQMLDLYSAKPKCIPGINFMKLLGEDEEAFDVLYCLAFEMMDAQWLAMHASYMEFNVIF 351

Query: 253 ---------------------------------QETRAQLERELSLDDNKTR-----EIE 312
                                            Q TR    + +    NK R      ++
Sbjct: 352 ISISPFPYEHRKLRGSTSHANAIGKRAFFRRCTQSTRLTGLQSVVPVKNKRRASKLWNVK 411

Query: 313 SERQREMAT--KETEET--------------------EELLKGQAEIWKYMLCFADSMAL 372
             R  ++ T  K+   T                    + +L+GQ EIWKYML FADSMAL
Sbjct: 412 RSRWVQIFTSSKQLSSTMLIWGLIEGNARCCIFLDGEDAVLRGQVEIWKYMLSFADSMAL 471

Query: 373 KCAVELHLADIINSHGSPISLSQIASSIVASDPLATPEISYLSRIMRLLVRRNIFAVHH- 432
           KCAVEL LADII+SH SPI+LSQIAS+I  +    +P++S L+RIMRLLVRR IF  H  
Sbjct: 472 KCAVELQLADIIHSHTSPITLSQIASAIPGA---TSPDLSCLARIMRLLVRRRIFTQHQK 531

Query: 433 -SSDG--GEPLYGLTHSSKWLLRDA----ELTLAPMVLAELHKWMVDPWLCFSRGVKEGG 492
             SDG   E LYG THSS+WLL       +LTLAPM+L E    ++ PW CFSR VKE  
Sbjct: 532 PKSDGEEEEALYGPTHSSRWLLTKTNDHDQLTLAPMILMENDPRLMAPWHCFSRCVKEA- 591

Query: 493 DQFQKANGLDIWSFASTNPQFNQLFNNAMASTSKVIMKAILSVYQD-GFSSIESLTDVGG 552
                 NG  IW F + NP+ N+LFN+AM  T+KV+MKAILS Y D GFS I+S+ DVGG
Sbjct: 592 -----HNGQSIWEFGAENPEINKLFNDAMECTAKVVMKAILSHYSDGGFSDIKSMVDVGG 651

Query: 553 GIGGVISEIVKAFPHIKGMNFDLPHVISTAPLYQGVTHIGGDMFEAIPKADAVFMKWILH 609
           G GG ISEIV+++PHIKG+NFDLPHVI+TAP Y GV+H+GGDMF ++P ADA+FMK    
Sbjct: 652 GTGGSISEIVRSYPHIKGINFDLPHVIATAPPYSGVSHVGGDMFRSVPTADAIFMK---- 711

BLAST of Carg13574 vs. ExPASy TrEMBL
Match: A0A5A7V8T5 ((R,S)-reticuline 7-O-methyltransferase-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold143G001350 PE=3 SV=1)

HSP 1 Score: 603.6 bits (1555), Expect = 9.6e-169
Identity = 286/357 (80.11%), Postives = 320/357 (89.64%), Query Frame = 0

Query: 281 MATKETEETEELLKGQAEIWKYMLCFADSMALKCAVELHLADIINSHGSPISLSQIASSI 340
           M  KET E E LL+GQAEIWKYMLCFADSMALKCAVELHLADIINSHGSPISLSQI+SSI
Sbjct: 1   MEAKETNEAEALLQGQAEIWKYMLCFADSMALKCAVELHLADIINSHGSPISLSQISSSI 60

Query: 341 VASDPLATPEISYLSRIMRLLVRRNIFAVHHSSDGGEPLYGLTHSSKWLLRDAELTLAPM 400
            AS+P ++P+ISYL+RIMRLLVRRNIFA HH SDGG+ LYGLTHSSKWLLRD+ LTLAPM
Sbjct: 61  AASNPSSSPQISYLNRIMRLLVRRNIFAAHHPSDGGDTLYGLTHSSKWLLRDSPLTLAPM 120

Query: 401 VLAELHKWMVDPWLCFSRGVKEGGDQFQKANGLDIWSFASTNPQFNQLFNNAMASTSKVI 460
             +ELH+WMV PWLCF+  VKEGG  F+ A+GLDIW FAS NPQFN  FN+AMASTSKV+
Sbjct: 121 AFSELHQWMVSPWLCFTEAVKEGGSPFKIAHGLDIWDFASKNPQFNHFFNDAMASTSKVV 180

Query: 461 MKAILSVYQDGFSSIESLTDVGGGIGGVISEIVKAFPHIKGMNFDLPHVISTAPLYQGVT 520
           M AILSVYQDGF+S++SL DVGGGIGG ISEIVKAFPHIKG+N+DLPHVISTAP+Y+GVT
Sbjct: 181 MNAILSVYQDGFNSLDSLADVGGGIGGSISEIVKAFPHIKGINYDLPHVISTAPVYEGVT 240

Query: 521 HIGGDMFEAIPKADAVFMKWILHDWDDKECVRILKNCKKAIPEKTGKVILVEVILKADGD 580
           HIGGDMFE IPK DA+FMKWILHDW+DKECV+IL+NCKKAIPEK GKVI+VEV+L  +G 
Sbjct: 241 HIGGDMFEDIPKVDAIFMKWILHDWNDKECVKILENCKKAIPEKRGKVIIVEVVLNEEGK 300

Query: 581 GAFDDTRFYFDLLMLAHTNGRERTEKEWKNILEEAGFPRYRVIALPAITSIIEAYPS 638
           GAFDDT+FYFDLLMLAHTNG+ERTEKEWK ILEEAGF RY +I LPA+ SIIEAYPS
Sbjct: 301 GAFDDTKFYFDLLMLAHTNGKERTEKEWKTILEEAGFSRYNLIPLPALVSIIEAYPS 357

BLAST of Carg13574 vs. ExPASy TrEMBL
Match: A0A1S3BJA7 ((R,S)-reticuline 7-O-methyltransferase-like OS=Cucumis melo OX=3656 GN=LOC103490512 PE=3 SV=1)

HSP 1 Score: 603.6 bits (1555), Expect = 9.6e-169
Identity = 286/357 (80.11%), Postives = 320/357 (89.64%), Query Frame = 0

Query: 281 MATKETEETEELLKGQAEIWKYMLCFADSMALKCAVELHLADIINSHGSPISLSQIASSI 340
           M  KET E E LL+GQAEIWKYMLCFADSMALKCAVELHLADIINSHGSPISLSQI+SSI
Sbjct: 1   MEAKETNEAEALLQGQAEIWKYMLCFADSMALKCAVELHLADIINSHGSPISLSQISSSI 60

Query: 341 VASDPLATPEISYLSRIMRLLVRRNIFAVHHSSDGGEPLYGLTHSSKWLLRDAELTLAPM 400
            AS+P ++P+ISYL+RIMRLLVRRNIFA HH SDGG+ LYGLTHSSKWLLRD+ LTLAPM
Sbjct: 61  AASNPSSSPQISYLNRIMRLLVRRNIFAAHHPSDGGDTLYGLTHSSKWLLRDSPLTLAPM 120

Query: 401 VLAELHKWMVDPWLCFSRGVKEGGDQFQKANGLDIWSFASTNPQFNQLFNNAMASTSKVI 460
             +ELH+WMV PWLCF+  VKEGG  F+ A+GLDIW FAS NPQFN  FN+AMASTSKV+
Sbjct: 121 AFSELHQWMVSPWLCFTEAVKEGGSPFKIAHGLDIWDFASKNPQFNHFFNDAMASTSKVV 180

Query: 461 MKAILSVYQDGFSSIESLTDVGGGIGGVISEIVKAFPHIKGMNFDLPHVISTAPLYQGVT 520
           M AILSVYQDGF+S++SL DVGGGIGG ISEIVKAFPHIKG+N+DLPHVISTAP+Y+GVT
Sbjct: 181 MNAILSVYQDGFNSLDSLADVGGGIGGSISEIVKAFPHIKGINYDLPHVISTAPVYEGVT 240

Query: 521 HIGGDMFEAIPKADAVFMKWILHDWDDKECVRILKNCKKAIPEKTGKVILVEVILKADGD 580
           HIGGDMFE IPK DA+FMKWILHDW+DKECV+IL+NCKKAIPEK GKVI+VEV+L  +G 
Sbjct: 241 HIGGDMFEDIPKVDAIFMKWILHDWNDKECVKILENCKKAIPEKRGKVIIVEVVLNEEGK 300

Query: 581 GAFDDTRFYFDLLMLAHTNGRERTEKEWKNILEEAGFPRYRVIALPAITSIIEAYPS 638
           GAFDDT+FYFDLLMLAHTNG+ERTEKEWK ILEEAGF RY +I LPA+ SIIEAYPS
Sbjct: 301 GAFDDTKFYFDLLMLAHTNGKERTEKEWKTILEEAGFSRYNLIPLPALVSIIEAYPS 357

BLAST of Carg13574 vs. TAIR 10
Match: AT1G03620.1 (ELMO/CED-12 family protein )

HSP 1 Score: 357.1 bits (915), Expect = 3.0e-98
Identity = 170/250 (68.00%), Postives = 208/250 (83.20%), Query Frame = 0

Query: 21  RQRRGDEELEWSHRSTPVILQLAQCFTNA--MVGPRTWIGGLFNRAGNRKNDKFVQYSLS 80
           ++ +G+++   S   +  ++ L +    A  MVG R+WIGGLF R+ NR+ DK V Y+LS
Sbjct: 2   KRGKGEKKATKSRDGSGQVVPLTEPVVTATGMVGTRSWIGGLFTRS-NRRQDKAVDYTLS 61

Query: 81  PLQEERFQRLQDRIPIPFDETQLEHQEALKALWDAAFPNIELKGMVSEQWKEMGWQGPNP 140
           PLQEER QRLQDR+ +PFDET+ +HQE+LKALW+ AFPN+ L G+V+EQWKEMGWQGPNP
Sbjct: 62  PLQEERLQRLQDRMVVPFDETRPDHQESLKALWNVAFPNVHLTGLVTEQWKEMGWQGPNP 121

Query: 141 STDFRGCGLISLENLLFFSRTFPASFRRLLLKEGGNRATWEYPFAVAGINISFMLIQMLD 200
           STDFRGCG I+LENLLF +RT+P  FRRLLLK+ G+RA WEYPFAVAGINISFMLIQMLD
Sbjct: 122 STDFRGCGFIALENLLFSARTYPVCFRRLLLKQRGDRAKWEYPFAVAGINISFMLIQMLD 181

Query: 201 L-NSERPRCLPGLNFVRLLGENEEAFDILYCVAFKMMDAQWVAMHASYMEFNEVLQETRA 260
           L N+ +P+CLPG+NF++LL E+E AFD+LYC+AF MMDAQW+AMHASYMEFNEVLQ TR 
Sbjct: 182 LQNNPKPKCLPGMNFLKLLEEDERAFDVLYCIAFAMMDAQWLAMHASYMEFNEVLQATRN 241

Query: 261 QLERELSLDD 268
           QLERELSLDD
Sbjct: 242 QLERELSLDD 250

BLAST of Carg13574 vs. TAIR 10
Match: AT3G03610.1 (ELMO/CED-12 family protein )

HSP 1 Score: 273.9 bits (699), Expect = 3.3e-73
Identity = 137/256 (53.52%), Postives = 178/256 (69.53%), Query Frame = 0

Query: 17  EIYWRQRRGDEELEWSHRSTPVILQLAQCFTNAMVGPRTWIGGL-----FNRAGNRKNDK 76
           E  W + R  +++ W+   + +I Q AQ     + G  + +G       F + G     +
Sbjct: 55  EDIWEEERKRQQVHWTLIFSQLIAQWAQWIAKIVFGSGSLVGRFLSLPTFGQIGT--GGR 114

Query: 77  FVQYSLSPLQEERFQRLQDRIPIPFDETQLEHQEALKALWDAAFPNIELKGMVSEQWKEM 136
            +   LS LQEER + ++ RI IPFD +++EHQ+AL+ LW  A+P  EL  + SE WKEM
Sbjct: 115 LLPPPLSMLQEERLRNIKRRIEIPFDGSRMEHQDALRQLWRLAYPQRELPPLKSELWKEM 174

Query: 137 GWQGPNPSTDFRGCGLISLENLLFFSRTFPASFRRLLLKEGGNRATWEYPFAVAGINISF 196
           GWQG +PSTDFRG G ISLENL+FF++T+P SF+RLL K+ G RA WEYPFAVAGINISF
Sbjct: 175 GWQGTDPSTDFRGGGYISLENLIFFAKTYPESFQRLLHKQDGTRAEWEYPFAVAGINISF 234

Query: 197 MLIQMLDLNSERPRCLPGLNFVRLLGENEEAFDILYCVAFKMMDAQWVAMHASYMEFNEV 256
           ML QMLDL S +P  + G+ F+  L E+E AFD LYC+AF+MMDAQW+A  ASYMEFN+V
Sbjct: 235 MLAQMLDLQSGKPSTIAGIRFLGFLEEDEMAFDNLYCIAFQMMDAQWLARRASYMEFNDV 294

Query: 257 LQETRAQLERELSLDD 268
           L+ TRAQLEREL+LDD
Sbjct: 295 LKSTRAQLERELALDD 308

BLAST of Carg13574 vs. TAIR 10
Match: AT4G35160.1 (O-methyltransferase family protein )

HSP 1 Score: 267.7 bits (683), Expect = 2.4e-71
Identity = 149/379 (39.31%), Postives = 221/379 (58.31%), Query Frame = 0

Query: 278 QREMATKETEETEELLKGQAEIWKYMLCFADSMALKCAVELHLADIINSHGS--PISLSQ 337
           + +M   + +  +E  K   +IWKY+  FAD  A KCA++L + + I +H S  P++L++
Sbjct: 12  RNKMEDNKRKVLDEEAKASLDIWKYVFGFADIAAAKCAIDLKIPEAIENHPSSQPVTLAE 71

Query: 338 IASSIVASDPLATPEISYLSRIMRLLVRRNIFAVHHSSDGGEPLYGLTHSSKWLL---RD 397
           ++S++ AS        S+L RIMR LV + IF    + DG    Y  T  S+ L+   RD
Sbjct: 72  LSSAVSASP-------SHLRRIMRFLVHQGIFKEIPTKDGLATGYVNTPLSRRLMITRRD 131

Query: 398 AELTLAPMVLAELHKWMVDPWLCFSRGVKEGGD-----QFQKANGLDIWSFASTNPQFNQ 457
            + +LAP VL E    M+ PWL  S  V    +      F   +G D+WSFA  NP  + 
Sbjct: 132 GK-SLAPFVLFETTPEMLAPWLRLSSVVSSPVNGSTPPPFDAVHGKDVWSFAQDNPFLSD 191

Query: 458 LFNNAMASTSKVIMKAILSVYQDGFSSIESLTDVGGGIGGVISEIVKAFPHIKGMNFDLP 517
           + N AMA  ++ ++  +       F  + ++ DVGGG G  +  +VK FP IKG NFDLP
Sbjct: 192 MINEAMACDARRVVPRVAGACHGLFDGVTTMVDVGGGTGETMGMLVKEFPWIKGFNFDLP 251

Query: 518 HVISTAPLYQGVTHIGGDMFEAIPKADAVFMKWILHDWDDKECVRILKNCKKAIPEKTGK 577
           HVI  A +  GV ++ GDMF++IP  DA+F+KW+LHDW DK+C++ILKNCK+A+P   GK
Sbjct: 252 HVIEVAEVLDGVENVEGDMFDSIPACDAIFIKWVLHDWGDKDCIKILKNCKEAVPPNIGK 311

Query: 578 VILVEVILKA--------DGDGAFDDTRFYFDLLMLAHTN-GRERTEKEWKNILEEAGFP 637
           V++VE ++          + D   +  R   D++M+AHT+ G+ERT KEW  +L+EAGF 
Sbjct: 312 VLIVESVIGENKKTMIVDERDEKLEHVRLMLDMVMMAHTSTGKERTLKEWDFVLKEAGFA 371

BLAST of Carg13574 vs. TAIR 10
Match: AT3G60260.3 (ELMO/CED-12 family protein )

HSP 1 Score: 264.6 bits (675), Expect = 2.0e-70
Identity = 127/223 (56.95%), Postives = 160/223 (71.75%), Query Frame = 0

Query: 47  TNAMVGPRTWIGGLFN--RAGNRKNDKFVQYSLSPLQEERFQRLQDRIPIPFDETQLEHQ 106
           +  + G   W+G   +      R  D    + L+P QEE  QRLQ RI + +D +  +HQ
Sbjct: 7   SEVVAGSAAWLGRGLSCVCVQGRDGDPRPSFDLTPAQEECLQRLQSRIDVAYDSSIPQHQ 66

Query: 107 EALKALWDAAFPNIELKGMVSEQWKEMGWQGPNPSTDFRGCGLISLENLLFFSRTFPASF 166
           EALK LW  AFP  EL G+VS+QWKEMGWQG +PSTDFRG G ISLENLL+F+R FP SF
Sbjct: 67  EALKDLWKLAFPEEELHGIVSDQWKEMGWQGKDPSTDFRGGGFISLENLLYFARKFPKSF 126

Query: 167 RRLLLKEGGNRATWEYPFAVAGINISFMLIQMLDLNSERPRCLPGLNFVRLLGENEEAFD 226
             LL K+ G+R+ WEYPFAVAGINI+FMLIQMLDL + +PR + G  F+R L  NE AFD
Sbjct: 127 HGLLRKQVGDRSVWEYPFAVAGINITFMLIQMLDLEAVKPRSIVGETFLRFLSVNESAFD 186

Query: 227 ILYCVAFKMMDAQWVAMHASYMEFNEVLQETRAQLERELSLDD 268
           +LYC+AFK+MD QW++M ASYMEFN V++ TR QLERE+ ++D
Sbjct: 187 LLYCIAFKLMDQQWLSMRASYMEFNTVMKSTRRQLEREIMIED 229

BLAST of Carg13574 vs. TAIR 10
Match: AT2G44770.1 (ELMO/CED-12 family protein )

HSP 1 Score: 264.2 bits (674), Expect = 2.6e-70
Identity = 127/221 (57.47%), Postives = 162/221 (73.30%), Query Frame = 0

Query: 49  AMVGPRTWIGGLFN--RAGNRKNDKFVQYSLSPLQEERFQRLQDRIPIPFDETQLEHQEA 108
           A+ G   W+G   +   A  R +D    + L+P QEE  Q LQ+RI + +D T   HQEA
Sbjct: 31  AVPGSAAWLGRGLSCVCAQRRDSDANSTFDLTPAQEECLQSLQNRIDVAYDSTIPLHQEA 90

Query: 109 LKALWDAAFPNIELKGMVSEQWKEMGWQGPNPSTDFRGCGLISLENLLFFSRTFPASFRR 168
           L+ LW  +FP  EL G++SEQWKEMGWQG +PSTDFRG G ISLENLL+F+R F  SF+ 
Sbjct: 91  LRELWKLSFPEEELHGLISEQWKEMGWQGKDPSTDFRGGGFISLENLLYFARNFQKSFQD 150

Query: 169 LLLKEGGNRATWEYPFAVAGINISFMLIQMLDLNSERPRCLPGLNFVRLLGENEEAFDIL 228
           LL K+ G+R+ WEYPFAVAGIN++FMLIQMLDL + +PR + G  F++ L ENE AFD+L
Sbjct: 151 LLRKQVGDRSVWEYPFAVAGINLTFMLIQMLDLEAVKPRTIVGATFLKFLSENESAFDLL 210

Query: 229 YCVAFKMMDAQWVAMHASYMEFNEVLQETRAQLERELSLDD 268
           YC+AFK+MD QW++M ASYMEFN V++ TR QLEREL L+D
Sbjct: 211 YCIAFKLMDQQWLSMRASYMEFNTVMKSTRRQLERELMLED 251

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
KAG7034926.10.0e+00100.00xanthohumol 4'-O-methyltransferase, partial [Cucurbita argyrosperma subsp. argyr... [more]
KAG6604808.11.9e-20399.72Xanthohumol 4-O-methyltransferase, partial [Cucurbita argyrosperma subsp. sorori... [more]
XP_022946951.15.2e-20198.87xanthohumol 4'-O-methyltransferase-like [Cucurbita moschata][more]
XP_023532623.12.0e-19796.92xanthohumol 4'-O-methyltransferase-like [Cucurbita pepo subsp. pepo][more]
XP_022970718.12.0e-19796.08xanthohumol 4'-O-methyltransferase-like [Cucurbita maxima][more]
Match NameE-valueIdentityDescription
B0ZB565.1e-11154.14Xanthohumol 4-O-methyltransferase OS=Humulus lupulus OX=3486 GN=OMT2 PE=1 SV=1[more]
Q6WUC23.2e-10552.69(R,S)-reticuline 7-O-methyltransferase OS=Papaver somniferum OX=3469 GN=7OMT PE=... [more]
B0ZB552.2e-10153.01Desmethylxanthohumol 6'-O-methyltransferase OS=Humulus lupulus OX=3486 GN=OMT1 P... [more]
Q9LEL62.8e-7742.17(RS)-norcoclaurine 6-O-methyltransferase OS=Coptis japonica OX=3442 PE=1 SV=1[more]
Q5C9L75.4e-7641.43(RS)-norcoclaurine 6-O-methyltransferase OS=Thalictrum flavum subsp. glaucum OX=... [more]
Match NameE-valueIdentityDescription
A0A6J1G5062.5e-20198.87xanthohumol 4'-O-methyltransferase-like OS=Cucurbita moschata OX=3662 GN=LOC1114... [more]
A0A6J1I4R19.8e-19896.08xanthohumol 4'-O-methyltransferase-like OS=Cucurbita maxima OX=3661 GN=LOC111469... [more]
A0A7J6HG985.6e-18553.87ELMO domain-containing protein OS=Cannabis sativa OX=3483 GN=G4B88_018487 PE=3 S... [more]
A0A5A7V8T59.6e-16980.11(R,S)-reticuline 7-O-methyltransferase-like OS=Cucumis melo var. makuwa OX=11946... [more]
A0A1S3BJA79.6e-16980.11(R,S)-reticuline 7-O-methyltransferase-like OS=Cucumis melo OX=3656 GN=LOC103490... [more]
Match NameE-valueIdentityDescription
AT1G03620.13.0e-9868.00ELMO/CED-12 family protein [more]
AT3G03610.13.3e-7353.52ELMO/CED-12 family protein [more]
AT4G35160.12.4e-7139.31O-methyltransferase family protein [more]
AT3G60260.32.0e-7056.95ELMO/CED-12 family protein [more]
AT2G44770.12.6e-7057.47ELMO/CED-12 family protein [more]
InterPro
Analysis Name: InterPro Annotations of Silver-seed gourd (SMH-JMG-627) v2
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availableCOILSCoilCoilcoord: 249..269
NoneNo IPR availableGENE3D3.40.50.150Vaccinia Virus protein VP39coord: 391..637
e-value: 7.2E-82
score: 276.3
NoneNo IPR availablePIRSRPIRSR005739-1PIRSR005739-1coord: 309..635
e-value: 2.3E-95
score: 316.9
NoneNo IPR availablePANTHERPTHR11746:SF235(R,S)-RETICULINE 7-O-METHYLTRANSFERASE-LIKE ISOFORM X1coord: 285..636
IPR012967Plant methyltransferase dimerisationPFAMPF08100Dimerisationcoord: 310..362
e-value: 8.5E-13
score: 48.1
IPR006816ELMO domainPFAMPF04727ELMO_CED12coord: 87..252
e-value: 5.9E-46
score: 156.6
IPR006816ELMO domainPROSITEPS51335ELMOcoord: 102..263
score: 29.340445
IPR001077O-methyltransferase domainPFAMPF00891Methyltransf_2coord: 417..617
e-value: 1.0E-66
score: 224.2
IPR036388Winged helix-like DNA-binding domain superfamilyGENE3D1.10.10.10coord: 281..389
e-value: 3.8E-35
score: 121.7
IPR016461O-methyltransferase COMT-typePANTHERPTHR11746O-METHYLTRANSFERASEcoord: 285..636
IPR016461O-methyltransferase COMT-typePROSITEPS51683SAM_OMT_IIcoord: 300..636
score: 79.888016
IPR029063S-adenosyl-L-methionine-dependent methyltransferaseSUPERFAMILY53335S-adenosyl-L-methionine-dependent methyltransferasescoord: 396..635
IPR036390Winged helix DNA-binding domain superfamilySUPERFAMILY46785"Winged helix" DNA-binding domaincoord: 289..392

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Carg13574-RACarg13574-RAmRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0019438 aromatic compound biosynthetic process
biological_process GO:0032259 methylation
molecular_function GO:0050630 (iso)eugenol O-methyltransferase activity
molecular_function GO:0046983 protein dimerization activity
molecular_function GO:0102719 S-adenosyl-L-methionine:eugenol-O-methyltransferase activity
molecular_function GO:0008757 S-adenosylmethionine-dependent methyltransferase activity
molecular_function GO:0008168 methyltransferase activity
molecular_function GO:0008171 O-methyltransferase activity